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عزل مركب فينولي من نبات الصبار barbadensis Aloe وبلمرته ودراسة بعض تطبيقاتها الحيوية == Isolation of phenolic compound from Aloe Vera Plant (Aloe barbadensis) and it,s polymerization and study some of it,s biological applicationa

Author name: عبير سلمان عبود جاسم
Supervisor name: فاطمة صيوان صباح | مؤيد نعيم خلف
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضـــير وتشخـيص ودراسة تحليلية لبعض الصبغات الازوية الجديدة المشتقة من الاورثوفانيلين ومعقداتها مع ZN 11 Cd II == Preparation , identification and analytical Study of some new azo dyes derived form o-vanillin and their complexes with Zn(II) , Cd(II)

Author name: عبد الشهيد هندي بطاي
Supervisor name: اسعد عبود علي | طارق علي الفهد
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تقدير بعض الايونات السالبة والموجبة بواساطة نظام ايون مرومتوغرافي مصمم محليا == Determination of Some Anions and Cations by Home-made Ion Chromatography System

Author name: خولة سلمان عبد الرسول
Supervisor name: كامل حسين علوان السوداني
General topic: Chemistry
Specific topic: Chemistry
Degree: Doctorate
Language: Arabic
University location: Basrah

تحضير تراكيب نانوية لثنائي اوكسيد التيتانيوم واوكسيد الزنك ودراستها في النقل الدوائي والتكسير الضوئي == Preparation of Nanoparticles for Titanium Dioxide and Zinc Oxides and their Study in Drug Release and Photocatalysis

Author name: حوراء قاسم منور
Supervisor name: زينب طه ياسين
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير وتشخيص بعض منشطات السطوح التوامية وتطبيقاتها == The Ministry of Higher Education and Scientific Research University of Basrah, College of Education for Pure Sciences Chemistry Department

Author name: ايات علاء موسى
Supervisor name: مهند جواد كاظم
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير تشخيص ودراسة بايولوجية لبعض ثنائي 1.2.3 ترايازول النيوكليوسيدات اللاحلقية غير المتجانسة الجديدة == Synthesis , Identification and biological study of some new bis1,2,3-triazole hetero acyclonucleosi

Author name: همسة حسين خير الله الحجاج
Supervisor name: فائزة عبد الكريم ناصر
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير وتشخيص ودراسة تحليلية لراتنجات كلابية جديدة == Synthesis, characterization and analytical studies of new chelating Polymers

Author name: لمى طاهر طعمة البعاج
Supervisor name: طارق زباري جاسم
General topic: Chemistry
Specific topic: Chemistry
Degree: Doctorate
Language: English
University location: Basrah

تصميم نظام جريان متوقف لتقدير المغذيات في نماذج المياه == Designing a Stop-Flow System for Nutrient Determination in Water Samples

Author name: محمـد ذياب خليف البعلاوي
Supervisor name: كامل حسين علوان السوداني
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير تشخيص ودراسة بايلوجية لبعض مركبات الايزوكسازولدين الجديدة == Preparation, Charactrisation and Biological Study of some new isoxazolidines

Author name: رائد جميل علي الموسوي
Supervisor name: داخل زغير مطلق
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير ودراسة بايلوجية ونظرية لبعض مركبات التلوريوم العضوية الجديدة

Author name: رافد حميدان الاسدي
Supervisor name: طارق علي فهد | بهجت علي سعيد
General topic: Chemistry
Specific topic: Chemistry
Degree: Doctorate
Language: English
University location: Basrah

تحضير بعض منشطات السطوح البوليمرية الامفوتيرية التوامية الجديدة المشتقة من الاثلين ثنائي الامين رباعي حامض الخليك وتشخيصها ودراستها == Synthesis and Charactaztion of some New Gemini AmpHortic Polymeric Surfactants from Ethylenediamin tetraacetic acid

Author name: حيدر داود احمد العيسى
Supervisor name: محمود شاكر حسين
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

دراسة الخصائص الطيفية والذوبانية لبعض المعقدات الضمنية المحضرة من قواعد شف المشتقة من السلفا مع بعض الدكسترينات الحلقية == Spectroscopic study and Phase Solubility of Some Inclusion complexes of Sulfa Schiff bases with Some Cyclodextries

Author name: ايات عبد الكاظم مزهر
Supervisor name: جبار صالح هادي
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

دراسة التنوع الاحيائي النباتي في منطقة وادي الطيب شمال شرق العمراة == Study of Plant Biodiversity in Wadi Al-Tib Region North East of Ammara

Author name: سهاد عبد السادة طه الكنعاني
Supervisor name: عبد الرضا اكبر علوان المياح | ايمان محمد عبد الزهرة
General topic: Biology
Specific topic: Plant - Classification
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

الديدان المتطفلة في بعض الطيور المائية في مدينة الفاو جنوب شرق محافظة البصرة العراق == Helminthes parasitizing on some aquatic birds In the Faw town south eastern of Basrah Governorate, Iraq

Author name: اماني مؤيد ياسين
Supervisor name: باسم هاشم عبد الله
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تحضير بوليمر قابل لمتاكل الحيوي من حامض اللاكتيك باستخدام البكتريا المنتجة لحامض اللاكتيك == Preparation of Biodegradable Polymer of Lactic Acid using Lactic Acid Producing Bacteria

Author name: وصال عودة حسن الحلفي
Supervisor name: ميثم ايوب الحمداني | امال كاظم الاسدي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Basrah
First pages:

تقييم الحالة المناعية لمرضى الربو == Evaluation of Immunological Status of Asthmatic Patients

Author name: منى هادي صادق
Supervisor name: شيماء جبار
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تنقية وتوصيف انزيم الفيسين المستخلص من اوراق التين ودراسة كفاءته التحللية والتخثرية == PURIFICATION AND CHARACTERIZATION OF FICIN EXTRACTED FROM LEAVES OF FICUS AND STUDY IT,S PROTEOLYTIC AND CLOTTING ACTIVITY

Author name: مروة سلام سلمان العبيدي
Supervisor name: ام البشر حميد جابر الموسوي | روضة محمود العلي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: Arabic
University location: Basrah
First pages:

معالجة المياه العادمة باستخدام بعض التقانات الحياتية مع تقييم كفاءة محطة حمدان - البصرة == Wastewater Treatment by Using Some Technologies of Bioremediation and Evaluates the Efficiency of Hammdan Plant - Basrah

Author name: ماجدة صباح عبد السيد العنزي
Supervisor name: منال محمد اكبر | ابتسام مهدي عبد الصاحب
General topic: Biology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

دراسة بعض الجوانب الوبائية لداء اللشمانيا Leishmaniasis وتشخيص الطفيلي باستعمال تقنية تفاعل سلسلة البلمرة من النوع المتداخل Nested - Kinetoplast Minicircle DNA- PCR في محافظة ميسان ــ العراق == study of Epidemiolical aspects for Leishmaniasis and diagnosis of the Parasite by using Nested - Kinetoplast Minicircle DNA- PCR technique In the Province of Maysan - Iraq

Author name: حسن زعاطي عبادي ابو الدوانيج
Supervisor name: عبد الحسين حبش عواد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تشخيص وانتشار الحشرات المائية في برك مائية واقعة على نهر كرمة علي ودراسة التاثير السمي الحاد لبعض العناصر الثقيلة تجاه النوع Sympterum flaveolum. == Diagnostic and Distribution of aquatic insects in Ponds located in Garmat-Ali river and the Study of Acute Toxicity Effects of Some heavy Metals towards the Sympterum flaveolum

Author name: حامد منصور كاسب العونان
Supervisor name: ضياء خليف كريم | عماد هادي محسن
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة بيئة الهائمات النباتية في بعض محطات تصفية مياه الشرب في محافظة البصرة وتقدير تراكيز السموم الكبدية فيها == Ecological Study for Some Purification drinking water stations Phytoplankton in Basrah Governorate and Estimate the Concentration of Hepatotoxins

Author name: جنائن يعرب محمود
Supervisor name: عماد يوسف عواد
General topic: Biology
Specific topic: plant - aquatic life
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تاثير هرمون الهيدروكورتيزون على تمايز النسيج العظمي وبعض Mus التغيرات النسجية والفسيولوجية في اناث الفئران المختبرية musculus L. == Effect of Hydrocortisone hormone on the bone tissue differentiation and Some histological and physiological changes in females mice Mus musculus L.

Author name: الاء سالم كاظم الصيمري
Supervisor name: جاسم محمد جاسم | فراس شاكر كاطع
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تاثير نوع التغذية والضوء والتهوية في اقلمة ونمو نباتات الطماطم Lycopersicon esculentum Mil المكثرة نسيجيا == Effect of Nutrition types and Light, Ventilation on acclimation and growth of tomato (Lycopersicon esculentum Mill.) cultured in vitro

Author name: احمد عبد الشريف كاظم
Supervisor name: حسين خلف زاير | لمى حسين عبد القادر
General topic: Biology
Specific topic: Plant - Physiology
Degree: Master
Language: Arabic
University location: Basrah
First pages:

قابلية بعض انواع البكتريا المعزولة من مياه شط العرب والمياه البحرية على الامتزاز الحيوي لبعض المعادن الثقيلة == The Ability of Some Species of Bacteria Isolated From Shatt AL-Arab and Marine ateWr of Biosorption of Some Heavy Metals=

Author name: احمد جري شبيب الشمري
Supervisor name: غيداء جاسم الغزاوي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Basrah
First pages:

دراسة نظرية في التاثيرات البرمية الحرارية للنقطة الكمية في مجال مغناطيسي == Theoretical Study in the Thermospin Effects for the Quantum Dot in a Magnetic Field

Author name: ريمان جمال جاسم
Supervisor name: جنان مجيد المخ
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة نظرية للخواص الالكترونية والمغناطسية للحجم والسطح للمركبات MBi (M=V, Cr, Mn) والحد الفاصل MnBi/HgTe == A Theoretical Study of electronic and magnetic properties of the bulk and surface of compounds MBi (M= V, Cr, Mn) and the interfaces of MnBi/HgTe

Author name: نهاية هادي عبد الوهاب
Supervisor name: جبار منصور الزيادي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تحضير وتشخيص بعض مركبات البنزوثايوزول ودراسة صفاتها البلورية السائلة == Synthesis and Characterization of some Benzothiazole compounds and study their Liquid Crystalline properties

Author name: سجى عبد الحسين طالب
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير وتشخيص ودراسة الفعالية البايولوجية لبعض مشتقات الثايزولدين الجديدة == Synthesis, Characterization and Biological Activity Study of Some New Thiazolidine Derivatives

Author name: ايات ناجـــي حســــــن عــلي
Supervisor name: داود سالم عبد
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تخليق انابيب ثنائي اوكسيد التيتانيوم و جسيمات الفضة النانوية وتطبيقها في التحرر الدوائي و الفعالية الحيوية == Synthesis of Titanuim dioxid and Silver Nanoparticles and their applications in Drud Release and Bioactivity

Author name: احمد مكي صداع
Supervisor name: زينب طه ياسين
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

تحضير وتشخيص دايمرات متناظرة وغير متناظرة ودراسة صفاتها البلورية السائلة == Synthesis and Characterization of Symmetric and non - Symmetric Dimers and Study their Liquid Crystalline properties

Author name: ابتهال حميد شهيب
Supervisor name: عهود جبارعبيد الحمداني
General topic: Chemistry
Specific topic: Chemistry
Degree: Master
Language: Arabic
University location: Basrah

دراسة شكليائية الاسنان وتكوينها في سمكة المولي الاسود الشراعي Poecilia latipinna تحت تاثير هرمون الكالسيتونين

Author name: سارة طعمة رزاق السراي
Supervisor name: علي عبد اللطيف العلي
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة بعض التاثيرات الفسيولوجية والنسجية للمستخلص الايثانولي للطحلب ا لاخضر المزرق Oscillatoria limosa C. Agardh ex Gomont, 1892 في الفئران المختبرية البيض == Study of Some Physiological And Histological Effects of Ethanolic Extract of Alga Oscillatoria limosa C. Agardh ex Gomont, 1892 in laboratory white mice Mus musculus L

Author name: بان عبد المجيد اسماعيل
Supervisor name: فارس شاكر كاطع | احمد محسن عذبي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة تاثير المستخلص الايثانولي لطحلب Spirulina platensis على تراكيز الذهون وبعض المعايير الفسيولوجية في ذكور الارانب المختبرية المستحثة بفرط الكوليسترول

Author name: انتصار قاسم منور
Supervisor name: فارس شاكر كاطع | احمد حسن عذبي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:

الصفات المظهرية والتشريحية والكيميائية والوراثية لثلاث اصناف متباينة النضج من نخيل التمر Phoenix dactylifera L == Morphological, anatomical, chemical, and genetic characteristics of Three cultivars varying in maturity stage of Date Palm(Phoenix dactylifera L

Author name: يحيى نوري خلف
Supervisor name: كاظم جاسم حمادي | عبد الكريم محمد عبد
General topic: Biology
Specific topic: Plant - Anatomy
Degree: Doctorate
Language: English
University location: Basrah
First pages:

تاثير مبيد الميثيداثيون في بعض المعايير الفسيولجية والنسجية للفئران المختبرية البيض Mus musculus L. == Effect of Methidathion pesticide on some Physiological and Histological parameters of laboratory white mice Mus musculus L

Author name: ايمان حنش راهي
Supervisor name: سامي جبر المالكي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة تاثير بعض العناصر الثقيلة و الهيدروكاربونات النفطية الكلية على المعايير الحياتية والكيموحياتية في السرطان النهري Chiromentes boulengeri (Calman, 1920) في شط العرب == Study of Effect of Some Heavy Metals and Total Petroleum Hydrocarbons on Biological and Biochemical Parameters in the Crab Chiromentes boulengeri (Calman, 1920) In Shatt Al - Arab

Author name: انعام مهدي غضبان الطائي
Supervisor name: منال محمد اكبر الدركزلي | عبد الكريم طاهر يسر
General topic: Biology
Degree: Master
Language: Arabic
University location: Basrah
First pages:

التشخيص الجزيئي لطفيليات Sarcocystis spp في الجواميس Bubalus bubalisواختبار كفاءة مستضداتها في التشخيص المصلي و دورها في التهاب المفاصل الروماتويدي في الانسان == Molecular identification of Sarcocystis spp. parasites in Buffaloes (Bubalus bubalis) and evaluation of their antigens in serological diagnosis and their role in rheumatoid arthritis in human

Author name: هدى غانم داخل
Supervisor name: فوزية علي عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تقييم اداء تصاميم جديدة لسخان الماء الشمسي في مدينة البصرة30.5°N == Evaluation of new designs of solar water heater in Basrah city 30.5° N

Author name: كوثر كاظم منخي
Supervisor name: نوري حسين نور الهاشمي | جاسم مهدي الاسدي
General topic: Physics
Specific topic: Solar Energy Physics
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The main goal of the current study is the benefit of solar energy as the origin of any renewable energy for producing a hot water through multiple solar collectors each one with overall capacity about 26 liter so that, these collectors arranged in sequential order within the construction of the house without affecting the artistic aspects of the house. These collectors will capable of providing the house the needs of hot water for domestics use especially during the winter season. It will reduce the amount of energy such as electricity and or kerosene or any other source of energy that usually used in Basrah.The present study is included the design and manufacture of seven models of solar collectors. The tools that have used into manufactured of these collectors are some cheap materials that are available in Basrah markets. These models are differ from each other by some aspects with additions properties. The thermal performance and efficiency have a test and then choose the best one in order to use it in four different modern designs of solar heaters.the thermal performance of the manufactured heaters models are tested by calculate the amount of hot water supply under difference weathers conditions in the city of Basrah, which sorts as a large abundance of solar radiation. It also test two types of arrangement of difference parts of heaters. The practical test has been proven the advantage of linking hot water reservoir tank with cold water and then linking the whole system with the hot water tank. This arrangement will allow of gradual warming of the water, which allows the consumer get the hot water directly from the system and not from the reservoir. This study include what is called a feedback arrangement system in order to increase the benefit from solar radiation. In this case, the hot water will recycle to allow for moreexposure to solar radiation repeatedly, that in the case of non - use or the case of little use.All the experiment Steps carried out on the roof of the department of physics building's in the college of education for pure Sciences, Basrah university in the northern city of Basrah, located at latitude 30.50 N and longitude 47.80 E during the winter season 2013 - 2014. Al thought this winter characterized by abundant of rain and clouds the models No.5 shows very good results interm of high temperature of hot water and the amount of it. The temperature was reach (900 C). In addition the model No.4 which depend on the technique of feedback idea shows a good result interm of the amount of hot water produce which reach (146 liter) at a temperature about (500 C) with in only five hours starting at 10 : 00 am until the 2 : 00 pm.In order to develop the optimum dimensional measurements of the solar collectors a mathematical model was developed to predict the output temperature of hot water from the solar collectors or from the radiator in general for different dimensions. This model is working successfully and achieved data that in comparable with our experimental ones. The experimental results have been analyzed theoretically to compare the performance of all solar collectors of the heaters, in different weather conditions and in a same time.

درجة الحرارة المميزة وطاقة الاثارة الدنيا لليزر النقطة الكمية الشبه الموصل == Characteristic Temperature and Lowest Excitation Energy of Semiconductor QD laser

Author name: غيث منور علي
Supervisor name: رائد محمد حسن
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this work, we have studied important theoretical relationships in Semiconductor Quantum Dot Laser (QDL) Characteristics.The study is based on theoretical model equations that are derived from the rate equations. The equations were applied on experimental parameters of continuous - wave QDL GaInP/InP at 1.55μm wavelength to get theoretical simulation of the characteristics of this laser type.We also studied the temperature effect on the internal losses and the impact of these losses in determining the characteristics of the temperature of QD. The results were presented within two cases; the first is when there are no internal absorption losses and the second where there is no existence of these losses. Also, we studied the effect of ambient temperature on the characteristics of temperature for each of QD and OCL.The lowest excitation energy of QD has been studied in two control cases : with surface density of QD and with cross - section of internal losses.Throughout changing many systems and control parameters, the effects of these parameters have been studied according to the different kinds of relationships. In this research, we have studied the parameters : Surface density of QD, mean size of QD, optical confinement layer, radiative constant for OCL material, spontaneous radiative recombination time, root mean square of QD size fluctuations, cavity length and mirrors reflectivity.Some of the results in this work were compared with other experimental results of other researchers who applied the QDL model and showed a good matching.

التقنين الاشعاعي البيئي للمياه الجوفية في غرب محافظة البصرة باستخدام تقنية التالق الحراري == Environmental Radiation Dosimetry of Groundwater in West of Basra Governorate using Thermoluminescence Technique

Author name: سيف قاسم هلال
Supervisor name: رياض جاسب ابو الهيل
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study includes calculation the dose rate of emitted gamma - rays from groundwater taken from different locations in the Basra govemorate by use of thermoluminescence technique . it emphasis on the the characteristics of TL - reader ,TL - dosimeter (calcium fluoride) to know the suitability of these systems for environmental monitoring. It is found that the zero dose reading for calcium fluoride is 0.18 m rad .It is also found that most dosimeters of the same materials have nearly equal sensitivities and that all thebatches have high uniformity which makes them capable for environmental use. Experimental observations have indicated that there is linear relation between response and dose for the material which qualifies it for measuring doses within the low dose range. The variations in the response of the TL - reader using internal light source have also been investigated ,it is found that TL - reader has high stability .The fading in a environmental field for calcium fluoride is 7% in one month.Making sure that these systems are suitable for environmental monitoring programing ,calcium fluoride has been used for measuring gamma - rays emitting from natural background radiation ,it is foundthat the natural dose rate of gamma - rays emitted from the locations : Albrjisiya, khor aizubair, Safwan, Jabal Sanam, Um Qasr, Um Eanij and Al - lahees are (5.80,6.41, 5.32, 5.25,5.26,4.60 and 4.86) μrad/h receptivity . Thus, the dose rate of radiation emitted From ground water of province of Basra is 5.35 μ rad/h . the results proved that the inhabited area ling within the environmental monitoring satiations are the area in which the radiation is natural and that the rate that the human being exposed equal 11.5μrad/h which considered naturalexposure limit.

دراسة نظرية لانتشار نبضة كاوسية في الياف البلورة الفوتونية والعوامل المؤثرة فيها == Theoretical Study of the Propagation Gaussain Pulse in Photonic Crystal Fibers and the Parameters Influencing it

Author name: دعاء حسين هاشم
Supervisor name: حسن عبد الله سلطان
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this thesis the propagation of short Gaussian pulse in Photonic Crystal Fibers (PCFs) are studied, the effect of the fiber parameters on the pulse like the absorption, dispersion and nonlinearity studied also.Nonlinear Schrodinger Equation (NLSE) was solved numerically using the spectral or Split - Step Fourier Method (SSFM) to determine the pulse spatio - temporal evolution.The study includes the effect of the photonic crystal arrangement especially the holes number, hole - hole spacing and holes dimeter on the effective refractive index and the dispersion of the photonic crystal fiber.The Nonlinear Schrodinger Equation (NLSE) was solved using the Finite differences in the frequency domain (FDFD) method.The two zeros dispersion were studied to calculate the zero dispersion wavelength (ZDW) by applying the international golden ratio (1.16180339887) for the ratio of the hole - hole spacing to the holes dimeters.The study shows that the pulse propagation depends strongly on the absorption, dispersion and nonlinearity of the PCF, and the pulse wavelength must be near the ZDW to ensure the propagation with little loses.The dependence of the nonlinear refractive index, the dispersion and the ZDW of the PCF were proved.The study shows that the golden ratio can be used to have two ZDWs, and a new ratio of hole - hole spacing to the hole dimeter equal to 2 can be used to achieve two ZDWs.

حساب كثافة الحاملات في طبقة الحصر البصري واشغال الحاملات المحصورة في منظومة ليزر النقطة الكمية نوع GaInAsP/InP == Calculating the Carrier Density in OCL and Confined Carrier Occupation in Quantum Dot Laser System Type GaInAsP/InP

Author name: ایمان ذیاب شرشاب
Supervisor name: رائد محمد حسن
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this work, we have studied many control parameters effect which plays an important role to determine the density concentration of carriers in the Quantum Dot Laser (QDL) system. Our study is based on theoretical model equations which derived from the rate equations. The equations were applied to the GaInAsP/InP QDL with wavelength 1.55μm. We produce a theoretical simulation and analytical solution of the equations by a Mathematical program.Theoretical equations are used to study the effect the parameters by controlling the value of each : the carrier's density and occupancy of the Level of a carrier confined in a QD. There are two cases : when the cross - section of the internal absorption losses has a influential amount and a nather it case when approaching zero border. As well as, we study the impact of the parameters on the threshold current density and its compounds. In addition, we study the role of these parameter to determining the maximum temperature of QDL operation through effect on temperature rang of the system.The temperature effects on the carriers’ activities in the system are studied, whereas the characteristics temperature of QDL has been studied in the two cases : with / without internal losses.The effects of structure and control parameters are applied to Study many factors. Such as : surface density of QD, mean size of QDs, optical confinement layer, spontaneous radioactive recombination time and resonator losses.

دراسة مقارنة بين ليزرات الابار الكمية والنقاط الكمية == Comparative Study Between Quantum Wells and Quantum Dots Lasers

Author name: وطن حمود جبر
Supervisor name: جاسب عبد الحسين مشاري
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In the work we present a comprehensive comparison between the dynamics of quantum well and quantum dot semiconductor lasers working autonomously using two models, the first describe the temporal variation of carriers and photon densities respectively in the quantum well lasers and the second describe the carriers occupancies in the wetting layer and quantum dot and photon occupancy in the quantum dot lasers .The study was carried out on the delay time and level of the dc part of their power under the effect of the many parameters that appeared in those models. Both lasers dynamics were studied in the presence of injection current modulation which enhances lasers power range from periodic to chaotic one with varieties of periodic states such as period.1, period.2, period.3, period.4 and period.6. Developed chaos was noticed to occur.We propose and investigate a simple method to obtain complex output power from both lasers via pulse shaping of the injection current. During the three methods studied we noticed similarities and discrepancies in both lasers dynamics.

دراسة نظرية للتركيب الالكتروني الحجمي والسطحي لسبيكة نصف هيوسلر FeCrSe والحد الفاصل (111) FeCrSe/GaP == Theoretical Study of the Bulk and Surface Electronic Structure of the Half Heusler Alloy FeCrSe and the Interface of FeCrSe/GaP(111

Author name: مظاهر حبيب جولان الاسدي
Supervisor name: Mudhahir H. Jolan
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: قدمنا في هذه الدراسة ايضاحات مهمة حول الخواص المغناطيسية والتركيب الالكتروني في سبيكة نصف هيوسلر FeCrSe. وقد بينت النتائج ان FeCrSe هي مادة فيرومغناطيسية نصف معدنية عند ثابت الشبيكة المتوازن هو 5.506Å، وان العزم المغناطيسي الكلي المحسوب هو 2μB يخضع تماما لقواعد سليتر - بولنك. بالاضافة الى ذلك، بحثنا على نطاق واسع الخواص الالكترونية والمغناطيسية والنصف معدنية للسطوح (001) و(111) لسبيكة نصف هيوسلر FeCrSe والحد الفاصل مع القاعدة GaP (111) باستعمال حسابات المبادئ الاولية ضمن نظرية الكثافة الدالية. وتوضح كثافة الحالات الذرية ان الصفة النصف معدنية المثبتة في الحجم FeCrSe تكون محفوظة عند السطح (001) المنتهي بـ CrSe - والسطح (111) المنتهي بـ Se - ، ولكنها فقدت عند السطوح (111) المنتهية بـ Fe - وCr - والسطح (001) المنتهي بـ Fe - . علاوة على ذلك، للحد الفاصل FeCrSe/GaP (111)، تكون الصفة النصف معدنية للحجم محطمة عند الهيئة Se - P بينما يبين الحد الفاصل والحد الثانوي الفاصل Se - Ga تقريبا استقطاب برم 100%. ضمن هذه الدراسة اوضحنا، من حسابات طاقات التلاصق (γ) للحد الفاصل ان الشكل او الهيئة Se - Ga هو اكثر استقرارا من الاخر Se - P. وايضا درسنا العزوم المغناطيسية، فلاحظنزايادة العزوم المغناطيسية المحسوبة لـ Se وGa عند الحد الفاصل Se - Ga (111) وP عند الحد الفاصل Se - P (111) مقابل قيم الحجم المناظرة لها بينما العزم المغناطيسي الذري لذرة Se عند الحد الفاصل Se - P (111) يقل. ونلاحظ ايضا ان العزوم المغناطيسية للحد الفاصل الثانوي لذرة Fe عند كلا السطحين الفاصلين Se - Ga (111) وSe - P (111) تقل مقارنة بقيم الحجم. | In this study, important illustrations about the electronic structures and the magnetic properties of half - Heusler alloy FeCrSe.The results show that FeCrSe is half - metallic ferromagnet at equilibrium lattice constant (5.506 Å). The calculated total magnetic moment of 2.00 μB follows quite well the Slater - Pauling rule . We investigate extensively the electronic, magnetic, and half - metallic properties of the half - Heusler alloy FeCrSe (111) and (001) surfaces and the interface with GaP (111) substrate by using the first - principles calculations within the density functional theory. The atomic density of states demonstrates that the half - metallicity verified in the bulk FeCrSe is maintained at the CrSe - terminated (001) and Se - terminated (111) surfaces, but it is lost at both Cr - and Fe - terminated (111) surfaces and the Fe - terminated (001) surface. Furthermore, for the interface of FeCrSe/GaP (111), the bulk half - metallicity is destroyed at Se - P configuration while Se - Ga interface and subinterface show nearly 100% spin polarization. We explained within this study, the calculated interfacial adhesion energies exhibit that Se - Ga shape is more stable than the Se - P one. We also studied the magnetic moments, The calculated magnetic moments of Se, Ga at the Se - Ga (111) interface and P at the Se - P (111) interface increase with respect to the corresponding bulk values while the atomic magnetic moment of Se atom at the Se - P (111) interface decreases. We also notice that the magnetic moments of subinterface Fe at both Se - Ga and Se - P (111) interfaces decrease compared to the bulk values.

قياس مستوى الاشعاع الطبيعي في منطقة القبلة في محافظة البصرة == The Measurement of Natural Radiation Level in Qubla District in Basrah Governorate

Author name: محمد قاسم خضير
Supervisor name: عبد الرضا حسين صبر
General topic: Physics
Specific topic: Solar Energy Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The aims of this study is to measure the natural radioactivity in the selected areas from the province of Basrah using solid state nuclear tracks detectors SSNTDs both types of CR - 39 and LR - 115type Π to determine the concentration of radon gas. Gamma spectrometer Sodium Iodide NaI was used also to find specific activity of 238U, 232Th, 226Ra and 40K, in soil. This action is considered necessary from the point of view prevention of radiation hazard associated natural radioactivity of the soil in those areas and its impact on them. The first chapter includes an introduction to radiation and to shed light on the sources of radiation, natural and man made. Chapter 2 contains a highlight of radon gas and to identify the health effects of this gas as important sources of radiation in nature that can enter the human body through breathing. Chapter III contains the identification of the study area and samples collection of and preparation methods of the samples. The preparation of the measuring cylinder for measuring radon gas concentration at dwelling presented in the area. The natural gamma radioactivity measured by the NaI(Tl), also presented in the same chapter. This chapter also clarified; the equations used in calculations of radon gas concentration, the equations used to measure the specific activity of 238U, 232Th, 226Ra and 40K in selected soil samples taken from the area and assess the hazard indiceis caused by radioactivity (Raeq). Chpter 4, contains a review of methods used in the measurements includes materials and equipment used. Chapter five includes our findings from this study which is shows that, The radon gas concentration in dwelling has been measured found to be in the range of; 11.91 ± 1.3 Bq / m3 to 606.7 ± 66.52Bq / m3 with average value equal to 78.51Bq / m3, which is much less of the value specified by the United States Environmental Protection Agency EPA a 150Bq / m3 and this does not constitute a high risk to the health. The next part of this research; is the measurement of the natural gamma radiation emitted by the surface soil. We found that the lowest value for the concentration of isotope 226Ra is 2.5Bq / kg and maximum value was 147.4 Bq / kg with range of 47.3 Bq / kg. The minimum gamma concentration of 238U is 0.09 Bq/kg, while the maximum value is 94.7Bq / kg and an average of 23.5Bq / kg .The minimum concentration of 232Th is 1Bq / kg and maximum value of 83.9 Bq / kg at a range of 41.1Bq / kg. The minimum value of 40K was found to be 1 Bq / kg and the greatest value is 1048.6 Bq / kg and an average of 499.2 Bq / kg.All of these results are within the rangeof permitted and there is no rise in the value of the concentration of radioactive elements naturally calls for action to clean up pollution action cleaning , As for the soil of the region have indicated results found that the greater the intensity of the effects recorded in the soil of the area studied detector CR - 39 is 56187.5±3533.9 Track/cm2 while the lowest value was 1437.5±90.413 Track/cm2,which can be relied upon in radon concentration and content of radium account effective, but for the detector LR - 115TYPE it was found that the greatest density of the recorded Track 17250±1085 Track/cm2,while the less dense traces recorded Track 188±12Track/cm2 .

دراسة الخواص البصرية والكهربائية ومحدد القدرة البصري للصبغات العضوية المشوبة للاغشية البوليمرية == STUDY OF THE OPTICAL AND ELECTRICAL PROPERTIES,AND OPTICAL POWER LIMITING OF ORGANIC DYES DOPED POLYMER FILMS

Author name: فيصل صادق ثامر
Supervisor name: عماد الدين حسين علي السعيدي
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This thesis contains results of a extensive study for many important and fundamental properties of two organic dyes, these are Phenol Red and Light Green SF Yellowish. To obtain the dye doped polymer films used for our study, the Polymethylmethacrylate (PMMA) polymer was doped by each one of these dyes , at different concentrations : 0.01, 0.02, 0.03, 0.04, 0.05, 0.30, 0.40 mM . Effect of adding small amount of Iodine (I2) , as a dopant, to the constituents of the dye doped polymer films at concentration of 0.03 mM for each dye, on the optical and electrical properties of the polymer films, was also studied. Casting method was used for preparing the dye doped polymer films samples.The present work involves the study of the linear and the nonlinear optical properties, and the optical power limiting of the dye doped polymer films, as well as study of the electrical properties of these films.We have studied the optical properties of both the dye - doped polymer films and the dye - doped polymer films with Iodine (I2). The study includes the following parameters : absorbance (A), transmittance (T), reflectance (R),iiabsorption coefficient (α), extinction coefficient (k), refractive index (n), dielectric constant (ε) with its real (εr) and imaginary (εi) parts, optical conductivity (σopt.), electrical conductivity (σelec.), and optical energy band gap (Eg).The results of the optical properties show that the dye - doped polymer films having high transmittance (T) , low reflectance (R) , and low absorption coefficient (α ) , over the wavelengths of the visible spectrum, and these films have energy band gaps belong to the indirect transitions. While the dye - doped polymer films with added different doping ratio of Iodine (I2), have low transmittance (T) and an increase in the values of reflectance (R) and absorption coefficient (α ), particularly in the absorption regions over the wavelengths near the ultraviolet (UV) region of the the electromagnetic spectrum.To study the nonlinear optical properties of the dye - doped polymer films, the laser beam z - scan technique was used with solid state laser (SSL) operating at the continuous wave (CW) at 532 nm. The laser output can be varied within the range 0 - 100 mW. The obtained results showed that the samples of the dye - doped polymer films as well as the samples of the dye - doped polymer films with Iodine(I2) have a nonlinear refractive index with negative value (n2 < 0) (occurrence of the self - defocusing effect) and aiiinonlinear absorption coefficient with negative value also (β < 0) (occurrence of the saturable absorption).We have studied the optical power limiting for the prepared films samples , using the laser beam z - scan technique. We have obtained optical power limiting with good optical properties. The results revealed that the smallest value for optical power limiting threshold is 15 mW for the Phenol red dye doped polymer film sample at concentration of 0.03 mM with Iodine doping ratio 36.2 % of the dye weight.Also, The electrical properties of the prepared samples, the dye - doped polymer films and the dye - doped polymer films with Iodine (I2), have been studied using the current - voltage (I - V) characteristic for three temperature degree values , which are given to these samples, these are : 298 K, 308 K and 318 K. The values of the electrical conductivity (σ) and the electrical conductivity activation energy (Ea) of polymer film were calculated. The influence of changing the temperature on these parameters was studied.The results obtained from the present our study indicate that the two organic dyes, Phenol Red and Light Green SF Yellowish, are good candidates for optical applications and they could be used for the photonic devices, the photo - electronic devices , and also in the optical power limiting

دراسة الخواص البصرية والكهربائية لبوليمرPoly (3 - Hioxy Thiophene, 2 - 5 dily) (P3HT) وتاثير اضافة صبغة Orang G على تلك الخواص مع تطبيقه في صناعة الخلايا الشمسية == Study of the optical and electrical properties of Poly (3 - Hioxy Thiophene, 2 - 5 dily) (P3HT) and the effect of Orang G on the properties and it's application in Fabrication of solar cells

Author name: فاطمة حميد مالك التميمي
Supervisor name: وليد علي حسين
General topic: Physics
Specific topic: Solar Energy Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: تم في هذه الدراسة تحضير اغشية رقيقة من بوليمر P3HT غير المشوب والمشوب بصبغة Orang G وبنسب تشويب Vol(1%,3%,4%,5%,7%,10% ) وبوليمر PEDOT : PSS غير المشوب وقد حضرت الاغشية بطريقة طلاء البرم .Spin Coating شخصت الاغشية البوليميرية المحضرة بواسطة الاشعة السينية X - Ray والاشعة تحت الحمراء FT - IR . اظهرت نتائج فحص الاشعة السينية X - Ray ان النماذج المحضرة لبوليمر P3HT غير المشوب والمشوب، اذ تكون اغشية البوليمر P3HT غير المشوب ذات تركيب عشوائي ونتيجة التشويب بدات النماذج المحضرة تكون شبه بلورية نتيجة تاثير صبغة Orang G المستخدمة. كذلك شخصت النماذج المذكورة انفا بطيف الاشعة تحت الحمراء FT - IR اذ اظهرت النتائج ان لهذه الاغشية مجاميع فعالة واضحة. درست كذلك الخواص البصرية لجميع الاغشية المحضرة كالامتصاصية(A) والنفاذية (T) والانعكاسية R)) ضمن مدى الطيف (300 - 900nm)،ودرس ايضا معامل الامتصاص(α) ومعامل الخمود(K) ومعامل الانكسار(n) وثابت العزل الحقيقي (ϵr) وثابت العزل الخيالي (ϵi) والتوصيلية البصرية (σopt) كدالة للطول الموجي . اظهرت الدراسة ان طيف الامتصاصية لاغشية المواد قيد الدراسة ضمن منطقة الطيف المرئي، حيث تم تسجيل اعظم قمة لبوليمر P3HT غير المشوب والمشوب بصبغة Orang G عند الطول الموجي 530 - 572nm)) وكتف (Shoulder) عند الطول الموجي 600nm .اظهرت الدراسة ان طيف الامتصاص لبوليمر PEDOT : PSS غير المشوب يكون ذات امتصاصية واطئة بحدود (0.1a.u) ويكون ذو نفاذية عالية تصل الى 85% .حسبت فجوة الطاقة البصرية للبوليمر P3HT غير المشوب فكانت (1.95eV) وتتناقص قيمة الفجوة مع زيادة نسبة التشويب حتى تصل الى (1.88eV)عند نسبة التشويب 5% Vol - (O.G) - P3HT،كذلك تزايد في كلا من معامل الامتصاص(α) ومعامل الخمود(k). تم حساب التوصيلية الكهربائية باستخدام ميزة(I - V)،ووجد ان التوصيلية الكهربائية تزداد مع زيادة درجة الحرارة والتي تكون ضمن مدى(30 - 70OC) وهو السلوك الغالب لجميع الاغشية المحضرة وهذا يدل انها اشباه الموصلات . تم حساب طاقة التنشيط لجميع الاغشية المحضرة ووجد ان طاقة التنشيط لبوليمر PEDOT : PSS تكون قليلة وهي بحدود (0.41eV) وهذا يفسر التوصيلية الجيدة لهذا البوليمر . ووجد ان طاقة التنشيط لبوليمر P3HT غير المشوب تكون بحدود0.6eV وعند نسبة التشويب بنسبة 1% Vol(O.G) - P3HT تصل طاقة التنشيط 0.4eV وبزيادة نسبة التشويب الى 5% Vol (O.G) - P3HT تصل طاقة تنشيطه الى 0.23 eV . استثمرنا النتائج التي تم الحصول عليها لعمل خلية شمسية ذات الطبقة الواحدة وحسبت معاملات الخلية الشمسية باستخدام ميزة (J - V) ووجد انها تكون ذات كفاءة تصل الى 0.1% عند نسبة التشويب 1% Vol(O.G) - P3HT وذات كفاءة 0.2% عند نسبة الشوائب 5% Vol(O.G) - P3HT Vol(O.G) - P3HT . | In this study thin films of un doped P3HT and (1%,3%,4%,5%,7%,10 %) Orang G doping P3HT and un doped PEDOT : PSS, have been prepared using spin coating method . The prepared films were characterized under XRD and FTIR .The XRD spectra shows an amorphous structures of the un doping which were become semicrystline as a results of doping . The FTIR spectra of un doping P3HT and pure (O.G) shows principle functional group as recorded in literature .The UV - IS optical properties related to absorbance (A), Transmit (T) and reflectance (R) in the rang (300 - 900) nm have been studies to and absorption coeffienct (α), refractive index (n), extinction coefficient (K) and the real dielectric constant (ϵr), and a imaginary dielectric constant (ϵi) as well as optical conductivity( σopt)funacton of Wavelength.In this found that un doping of P3HT and O.G doping thin films have broad bound peaks in the rang ( 530 - 572nm) with as shoulder at the wave length 600nm .PEDOT : PSS shows high transmittance of 85% . The calculated optical energy gap for un doping P3HT be (1.95eV) , and decreases with increasing O.G of doping reached to (1.88eV) at 5% Vol (O.G) P3HT doping .It is also that absorption coeffienct (α) and extinction coefficient (K), increasing as function of wave length (λ).The electrical conductivity (σ) due at (I - V) characteristic for un doping and O.G - doping found to be increases with increasing temperature for (30 - 70oC), for all sample . Shows is Organic semiconductors behavior .A activation energy of prepared thin films were estimated and found to be (0.41,0.6 ,0.4 eV) and 0.23 eV for PEDOT : PSS un doping P3HT ( 1% Vol (O.G) - P3HT ) ,(5% Vol( O,G) - P3HT) nespectivity . A inlayer solar cells wave constructers from the upper mention un doping and evaluated ,and found to have efficacies(0.1% ,0.2%) respectively.

دراسة نظرية للخواص النووية للانوية السوجية - السوجية 198 - 208Po و200 - 210Rn و218 - 228Ra و218 - 228Th و226 - 236U ا بسثعمال انموذج البوزونات المتفاعلة بنسختيه IBM - 1 وIBM - 2 == Theoretical Study for the Nuclear Properties of the even - even 198 - 208Po,200 - 210Rn,218 - 228Ra, 218 - 228Th,226 - 236U by using the Interacting Boson Model IBM - 1 , IBM - 2

Author name: علي فاخر حبيب
Supervisor name: عبد الرضا حسين صبر
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This work aims to study the details the nuclear structure of selected heavy nuclei 198 - 208Po ,200 - 210Rn, 218 - 228Ra, 218 - 218 - 228Th and 226 - 236U, due to the important of nuclear structure of these nuclei in applied nuclear physics. In this study two IBM version have been used, IBM - 1 and IBM - 2. The versions of model used to described the ground band, quizi - beta and quadruple reduced transition probability B(E2) for selected transition. Also, the IBM - 2 version has been used to calculate the magnetic reduce transition probability B(M1) and mixing ratio for group transition emitted from ground band, quazi - beta and quazi - gamma band . the mixed symmetry states and the effect of majorana parameters of the energy of these state have been studied extensively .the potential energy surface of each isotope has been produced as contour lines using the output of IBM - 1.From the result calculation it is found that there is an agreement between the available experimental data and the calculated results of both IBM - 1 and IBM - 2 . The agreement concerns the energies of ground band and less in the beta and gamma band for 198 - 208Po isotopes. Also we have agood agreement in values of B(E2) and B(M1) from the results of the two version. It is noticed that all the studied isotopes are belong to the vibrational U(5) limit.The result of 200 - 210Rn isotopes show closed to the vibrational U(5) moving toward the O(6) . the calculated values are very closed the experimental data for the positive parity states of the ground, beta and gamma bands with increasing of energy value near the closed shell.The variable position of 218 - 228Ra isotopes between the three limits of IBM - 1 ((SU(3), O(6), U(5)) ,where is; these isotopes moving towards rotational away from the closed shell . We notice the excellent agreement between calculated values and available experimental data for energy level and other nuclear properties. The value of E41/E21 for 218 - 228Th isotopes decreases with moving away from the closed shell, in the same time; the values of level energies decreases also. The agreement between the experimental and calculated values of energy level is very clear for energy level and B(E2) and B(M1) values.All studied 226 - 236U are belong to the rotational SU(3) limit and both versions of the model succeed in producing such limit and found agood agreement for all the bands specially the IBM - 2. It is found that the change in the Majorana parameter FS has an effect on the energy of the mixed symmetry state in studies isotopes . The B(E2) values also calculated and compared with experimental data.In the comparison between all the studied nuclei it is found that systematic of energy level of single nucleus and all isotopes it is found that the energy of the first excited state is different for such isotope and also the ratio of E41/E21 found to be moving toward rotational limit with increase of atomic number. In the present work we found that the ratio N/Z is always agree when we used different isotopes in the studied nuclei

دراسة الخواص البصرية والكهربائية لصبغات عضوية == Study of optical and electrical properties of organic dyes

Author name: علي ستار جبار الصيمري
Supervisor name: وليد علي حسين | فرات احمد مهدي
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this study, thin films of polyvinyl alcohol (PVA) doped with Orange G , Safranin O , and Crystal Violet dyes , have been prepared by using casting method after dissolving the dyes with distilled water .The linear optical parameters of the prepared thin films such as absorption coefficient (α) , extinction coefficient (k) , refractive index (no) and energy gap (Eg) , in addition to Urbach tails (Eu) , both real (εr) and imaginary (εi) parts of the dielectric constant , optical conductivity (σopt) , and electrical conductivity (σele) have been studied. The non linear absorption coefficient (β), non linear refractive index (n2) and the susceptibility of the third order (χ3) have been studied too .It is found that the absorption spectra of the prepared thin films are characterized by a clear absorption peaks in the visible range of the light spectrum . For Orange G two absorption peaks were appeared at the wavelengths (325 nm) and (490 nm) , where as one absorption peak is observed for each of Safranin O , and Crystal Violet at the wavelength (540nm) and (600nm) respectively. It is worth noting that the different concentrations of the dyes have an obvious traces on the absorption intensities of the thin films , which may be related to the extended Urbach tails as a result of increased dye concentration and (π - π*) transition .The estimated energy gaps gave an indication that an indirect allowed electronic transition take place and it is inversely slightly affected by the concentration of the dyes.The non linear optical properties for the prepared thin films were studied using Z - scan technique . Three different cases in which the materials are ; thedye solution consist of the dye dissolved in distilled water , the dye solution mixed with the polymer , and the dye doped thin polymer film , were studied using continuous wavelength (532 nm) solid state semiconductor laser type (SDL - 532 - 100T) .The results indicated that the calculated (β) and (n2) increases as a results of increase in the concentration for all the three mentioned cases . Defocusing due to thermal distribution through the samples which were accompanied with a changes in non linear refractive index were shown , that is , the samples behave like a thermal lens that distort the phase of the propagated beam in it .The (I - V) characteristics for all thin films give an indication of Ohmic behavior and the DC electrical conductivity (σele) increases as the temperature increase . The activation energies (Ea) were found to be decrease at higher dyes concentrations .

تحضير ودراسة بعض الخصائص الفيزيائية للمتراكب بولي انلين طين الكاؤولين العراقي == Preparation and study of the Some physical properties of imbricated Polly Aniline - IraqiAlca?lan clay

Author name: عباس عبد سويف البديري
Supervisor name: غفران محمد باقر شبيب
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: Polyanilin/Alcaulan Iraqi clay was modify by mecanochemical method with ideal condition and temperature range (0 - 5)0c after distillation Aniline (monomer) well.Several techniques have been used such as FT - IR ,X - ray to determine the crystalline ,scanning electron microscope (SEM) and finally (FAM) in order to study the surface roughness mediated .Many of physical properties such as mechanical properties which represented by hardness curves at different temperature which proved in hardness values range (2 - 7) for Alcaulan clay and (3 - 9.5) for PAni/Alcaulan while the results of tests fracture resistance (according to Brazilian way ) in different temperature was (2.9 - 4.92) for Alcaulan clay and (3.1 - 5.01) for PAni/Alcaulan .Optical properties show the type of electronic transition which found indirect transition and the optical energy gap about 2.15 eV.PAni/Alcaulan films was prepared by adding hydrochloric acid HCL after dissolved it in DMF as well as with addition to sulfuric acid H2SO4.The value of electrical conductivity of PAni - HCL/ Alcaulan are (0.4*10 - 7, 0.6*10 - 7, 0.7*10 - 7, 0.8*10 - 7, 0.9*10 - 7) s/cm. The value of PAni - H2SO4/Alcaulan are (4*10 - 7, 6*10 - 7, 6.5*10 - 7, 7.3*10 - 7, 9.1*10 - 7) s/cm.Also the value of electric conductivity of PAni - HCL/ Alcaulan with Iodine are (2*10 - 7, 6*10 - 7, 8*10 - 7) s/cm , and of PAni - H2SO4/Alcaulan are (19*10 -

تضمين البرم في ليزرات اشباه الموصلات (ليزرات البرم) == Spin Modulation in Semiconductor Lasers (Spin lasers

Author name: ضیاء جبار عكوش
Supervisor name: حسن عبد الله سلطان
General topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The introducing of electronic spin with its charge is the key of many applications which uses the electrical and magnetic properties of the electron , (spintronics )and semiconductor lasers.This concept gives led to the newest type of semiconductor lasers called ( Spin Lasers ).In the present thesis a numerical study of the effect of the carriers spin on the semiconductor lasers described by VCSEL using Matlab and Runge - Kutta method .The study includes the effect of the spin polarization in terms of the polarization injection and the injection current also to find the two thresholds of right polarized light circularly and the left circularly polarized one.The effect of the spin relaxation time on the dynamics of spin - VCSEL such as the steady state time evolution and direct modulation of the injection current and the polarization injection were studied also is presented too.The study shows that the spin VCSEL strongly affected by the injection current , polarization injection and spin relaxation time.The present results show that the laser threshold can reduced to quarter than that of conventional laser.

تحضير ودراسة بعض الخواص الكهربائية والبصرية للبوليمر الموصل بولي (انيلين اوكزالك) - وامكانية تطبيقاته في الخلايا الشمسية == preperation and studying some of the electrical and optical properties of conducting polymer poly(aniline - oxalic) and the possibility of its applications to solar cell

Author name: صفا نزار رمضان
Supervisor name: غفران محمد باقر شبيب
General topic: Physics
Specific topic: Solar Energy Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: We present in this study away of preparing Poly(aniline - oxali) (PAni - ox) by a Chemical polymerization with an ideal environment and temperature (5 - 0)0C. The Optical properties have been studied included the absorbance and transmitance measurements and calculations of the energy gap for (PAni - ox), which was found equal to (2.6eV). Electrical properties of (PAni - ox) have been also studied. The electrical conductivity for these materials was ( 8.22x10 - 2S.cm - 1) at room temperature.Thin films of titanium dioxide TiO2 of particle size (50 nm) and (25 nm) have been prepared and then deposited on a conductive glass bases Fluorine doped tin oxide (FTO) to study the optical properties.The materials under study have been diagnozid by using several techniques including X - rays (XRD) technique to determine the crystalline structures , Infrared (FT - IR) to determine the effective groups ,and Atomic Force Microscope (AFM) to determine the surface roughness and the regularity range of the nanoparticles compositions. Optical Anode was prepared by adding (PAni - ox) to TiO2, and the efficiency of the solar cell was calculated Two types of pigments alpha - Naphtholbenzein and EOSIN Y were added to TiO2 films and the prepared polymer is (PAni - ox). The pigments absorbs the solar energy and converte it to free electrons that are injected into titanium dioxide, which works as a collector for electrons. Electro solution was added to compensate for the loss electrons from the organic dye molecules. Thereafter, the electrode for the solar cell was prepared from carbon which has been obtained from candle flame.After preparation, the individual parts of solar cell have been collected by multi - layeres method . The (carrent - voltage) feature for cells by electrical properties have beem measured device and Light source.From this measurement , we calculate the open circuit voltage Voc and closed - circuit current Isc ,maximum power Pmax ,filling factor F.F, cell efficiency η, and Parallel resistance Rs and Resistance respectively Rsh.

الخواص البصرية اللاخطية ومحدد القدرة البصري للصبغات العضوية في المحاليل والاغشية البوليمرية باستخدام تقنية مسح حزمة الليزر على المحور z == Nonlinear Optical Properties and Optical Power Limiting of Organic Dyes in Solutions and Polymer Films Using The Laser Beam Z - Scan Technique

Author name: سيف الدين عبد الكريم قاسم
Supervisor name: عماد الدين حسين علي السعيدي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The research in this thesis is concerned with our extensive study of the spectral properties, the nonlinear optical properties, and the optical power limiting effect, for three organic dyes : Leishman, Giemsa, and Brilliant Cresyl Blue. Three different solvents were used to dissolve each dye : Chloroform, Ethanol, and Dimethyl(formamide) (DMF).We prepared dye solutions at four different concentrations : 0.03 mM, 0.05 mM, 0.07 mM, and 0.09 mM. Dye doped polymer films at concentration 0.09 mM were also prepared by mixing the dye with the polymer poly(methylmethacrylate) (PMMA) which already dissolved in the chloroform and using the casting method to obtain the desired polymer films.The linear absorption spectra of the dye solutions and the dye doped polymer films for different concentrations were analyzed by using the double - beam spectrophotometer in order to find the spectral range for each dye .2Our study was focused mainly on the nonlinear optical properties of the dye solutions and the dye doped polymer films. We employed in this study the laser beam z - scan technique and the solid - state laser (SSL) operating with the continuous wave (CW) at wavelength 532 nm and with variable output power over the range 0 - 100 mW .We have also studied the effect of the laser beam intensity on the nonlinear optical parameters of the dye solutions prepared by dissolving each dye in the Dimethyl(formamide) (DMF) solvent. Two values for the laser beam intensity were used for irradiating the samples (The dye solutions), these are : 1.11 kW / cm2 and 2.22 kW / cm2.Our obtained results from the present study reveal that all solutions of the three dyes and the dye - doped polymer films have a negative value for the nonlinear refractive index (n2 < 0) (occurrence of the self - defocusing effect) and also a negative value for the nonlinear absorption coefficient (β < 0) (saturable absorption).The values of the nonlinear optical parameters for each dye were calculated, these parameters are : The nonlinear refractive index (n2), the nonlinear absorption coefficient (β), and the absolute value of the nonlinear optical susceptibility (|χ(3)|). The solvents effect on the values of these optical3parameters was studied, and we find that the solvent plays an important role on the variation of these values.We also studied the optical power limiting for the dye solutions and the polymer films by using the laser beam z - scan technique. Good optical power limiting properties were obtained. The values of the optical power limiting threshold for the dye solutions at different concentrations and also for the dye doped polymer films were determind. We note that the value of the optical power limiting threshold depends on the kind of the used solvent for dissolving the dye. The smallest value for the optical power limiting threshold is 6.7 mW for the Brilliant Cresyl Blue dye doped polymer in chloroform at concentration 0.09 mM, while the smallest value for the power limiting threshold for the Brilliant Cresyl Blue solution in the same solvent at concentration 0.09 mM is 7.8 mW. The value of the power limiting threshold depends the kind of the used dye or the kind of the used solvent in preparing the sample and also on the concentration of the dye.The results that we have obtained from our present study show that the three dyes, Leishman, Giemsa, and Brilliant Cresyl Blue exhibit large nonlinear optical effects, suggesting that they are good promising materials for applications in the nonlinear photonic devices, the optical power limiting, and other optical devices.

تحضير اغشية رقيقة من اوكسيد الخارصين ZnO النقي والمشوب بالالمنيوم باستخدام تقنية المحلول ودراسة خواصها البصرية (Sol - Gel) الغروي وتطبيقها كمتحسس غازي == Preparation of Pure and Doped with Aluminum Zinc Oxide (ZnO) Thin Films by Using (Sol - Gel)Technique and the Study of Optical Properties and Applications as A Gas Sensor

Author name: سعد عبد الله جبر الفاضلي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this study, Zinc Oxide (ZnO) thin films are prepared by using Sol - Gel technique . The samples are prepared with different thicknesses (58,69,77)nm . Different weight ratio of Aqueous aluminum chloride (AlCl3.6H2O) are added to the Sol - Gel ZnOwith percentage (0.345%, 0.824% , 1.703% and 2.146%). These solutions are deposited by using spin coating on glass substrate .In order to investigate the sensitivity and the response of these samples for the detection about different vapors and gases (ethanol , methanol , acetone and nitrogen ) , pure and (AlCl3.6H2O) doped ZnO films are prepared by using the same technique but by adding Aluminum electrodes on these films with dimensions (2.5x0.5)cm2 .This preparing is followed by thermal treatment for these films by using oven with temperature reaches to 450 oC.The structure properties of these films are studied by using X - ray diffraction (XRD) and the results show that these films are polycrystalline and the calculated lattice constant are close to the standard values . The samples are analyses by Field Emission Scanning Electron Microscopic (FESEM) and the results show that the grain arrangement are regular and these structures are packed and the grain shape is as spherical . But for the AZO films , we notice that the increasing of the doping percentage reduces the gra in size .We also study the optical properties of these films such as Absorption (A) , Transmission (T) , Reflection (R) , Absorption coefficient (α) , extinction coefficient (k) , the energy gap(Eg) , refractive index (n) , with its complex and real dielectric constant( ) , dissipation factor (tanδ) , Optical Dispersion Parameters (Eo , Ed) and optical conductivity ( ) . The effect of the films thickness on the optical properties of the ZnO films for the over mentioned parameters . The results we get are discussed and compared with other studies . This work elucidates and analyzes the studying of the effect of adding (AlCl3 . 6H2O) o Zinc Oxide (ZnO) and the results are with standards agree with the results of many other researchers . The optical constant are tabulated and compared with , the results of other . And in order to determine the electronic transitions type , Absorption coefficient is calculated and it shows that ZnO and AZO films have transition of direct type because the values of α > 104 . The energy gap of the ZnO films with different thickness (58,69 and 77) nm is calculated , since it is found that the increasing of the film thickness gives slight increasing in the energy gap . The experimental results show that the increasing of doping percentagein ZnO film increases the energy gap with very little amount . In the electronic application field , the study also included design and fabrication of gas sensor system from local materials , which contain of chamber that made of plates polymer that contain of base for install the sample , electrodes to link the sample the measurement circuit , glass flask with three holes , heater , N2 gas cylinder , glass tubes , valves and the measurement circuit electric .The study also includes the ability of responding of the films for the thicknesses (58 , 69 and 77 )nm , to these gases , and it is found that the highest response was at vapor ethanol at the thickness 58nm. The effect of chloride Aluminum on the response of these percentage (0.345% , 0.824% , 1.703% , 2.146%) is studied for the mentioned gases , and it is found that the highest response we get is 45% at the concentration 2.146% for methanol gas . The results are tabulated , discussed and compared with precedent studies.

دراسة تراكيز الرادون والثورون والراديوم ومعدلات الانبعاث الكتلي والتبخر السطحي للرادون في البقوليات الجافة والعسل الطبيعي == Study of Radon , Thoron , Radium concentrations ,as well as the mass and surface exhalation rates in dry legumes and natural honey

Author name: سارة عبد اللطيف كديمي
Supervisor name: عيسى جاسم محمد الخليفة
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this study a sample of 50 different dry legumes and honey used (local and imported), the number of dry legumes samples under study were 21, including 5 samples of local and imported 16 samples collected from the Governorate of Basrah markets - Iraq. 29 samples of honey collected from local markets and apiaries in Iraq. The number of local honey 8 samples while the number of imported honey were 21 sample.Two methods in this research for various measurements and comparison between them. It is a method of (Misdaq and Satif.,1996) and the method of (Azam., 1995). Both methods are considered long - term methods for measuring the concentration of radon and thoron in various materials, Solid State Nuclear Track Detectors (SSNTDs), a closed cylinder technique method. Results in the first part of the samples dry legumes have shown that radon concentration ranges between a higher value (698.9Bq / m3) in thesample (Turkish lentil (imported)) and less valuable (69.0Bq / m3) in the samples (Iranian Homs and Chinese beans (imported)),and the concentration of thoron between the highest value (865.0Bq / m3) in the sample (Chinese beans (imported)) and the lowest value (86.45Bq / m3) in the sample (Canadian lentil (imported)), and that the radon concentration rate and thoron for samples local dry legumes (3551.1Bq / m3), (364.1Bq / m3), respectively. For samples imported dry legumes the radon concentration rate and thoron (333.0Bq / m3), (420.8Bq /m3 respectively For the results of the honey samples in the first part has found that the radon concentration between the highest value (826.7Bq / m3) in the samples ( AL - noor Honey (Basra - Seder) (Local)) , (Honey AngizaGerman (imported)) and less value (242.9Bq / m3) in the sample (Honey Mount Haj Omran (Local)), and the concentration of thoron between a higher value ( 538.4Bq / m3) in the sample (Turkish Honey) and the lowest value (115.2Bq / m3) in the sample (Honey Mount Haj Omran (Local)) , and that the concentration of radon and thoron samples of local honey rate (457.5Bq / m3), (297.0Bq / m3), respectively. For samples imported honey, the radon concentration and thoron rate(469.0Bq/m3) , (314.1Bq/m3) , respectively. Results obtained in the second part of the samples dry legumes note that the concentration of radium between the highest value of a (2.4489Bq / Kg) in the sample (Indian Homs (imported)) and less valuable (0.4030Bq / kg) in the sample (Indian Mash (Imported )), and that the concentration of radium samples local dry legumes rate (0.8941 Bq / Kg). For the samples dry legumes imported, the concentration of radium rate (0.9270Bq / Kg), the results obtained in the second part of the samples honey note that the concentration of radium ranges It is among the highest value (1.7270Bq / Kg) in the sample (Honey fall Karbala (Local)) and less valuable (0.2111Bq / Kg) in the sample (Honey Mount Haj Omran (Local)), and that the concentration of radium samples of local honey rate (0.7441 Bq / Kg). For the samples of imported honey, the concentration of radium rate (0.6141Bq / Kg).Results mass emission rates of radon samples dry legumes, ranging from the highest value (0.4442Bq.Kg - 1.d - 1) in the sample (Indian Homs (imported)) and the lowest value (0.0731Bq.Kg - 1.d - 1) in the sample (Indian Mash (imported)) , and the arithmetic average of the mass emission rate of the samples the local dry legumes (0.1540 Bq.Kg - 1.d - 1), and for dry legumes imported (0.1681 Bq.Kg - 1.d - 1), and the results of area emission rates radon samples dry legumes, ranging from the highestvalue (2.0405 Bq.m - 2.d - 1) in the samples (Indian Homs and Turkish lentil (imported)) and the lowest value (0.5681Bq.m - 2.d - 1) in the sample (Indian Mash (Imported)), and the arithmetic average of the rate of the area emission rate of surface samples local dry legumes (1.0865 Bq.m - 2.d - 1), and for samples of imported dry legumes (1.1589 Bq.m - 2.d - 1). Results mass emission rates of radon samples of honey, ranging from the highest value of ( 0.3132 Bq.Kg - 1.d - 1) in the sample (Honey fall Karbala (Local)) and the lowest value (0.0383Bq.Kg - 1.d - 1) in the sample (Honey Mount Haj Omran (Local)), and the arithmetic average of the mass emission rate for samples of local honey (0.1359 Bq.Kg - 1.d - 1), and for samples of imported honey (0.1114 Bq.Kg - 1.d - 1), and the results of area emission rates of radon samples of honey, ranging from the highest value (3.1286 Bq.m - 2.d - 1) in the sample (AL - noor Honey (Basra - Seder) (Local)) and the lowest value (0.6257 Bq.m - 2.d - 1) in the sample (Honey ount Haj Omran (Local)), and the arithmetic average of the rate of area ssion samples of local honey (1.4828 Bq.m - 2.d - 1), and for samples of imported honey (1.1670 Bq.m - 2.d - 1).

دراسة حركيات ليزر النقطة الكمية بوجود البرم == Study of Dynamics of Quantum Dot Laser with Spin

Author name: حكمت عبد الحسين ابراهيم
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The dynamics of VCSE QD spin polarized laser is studied based on asix equations model that takes into account the effect of number ofcontrol parameters on populations in the wetting layer for bothpolarized components and those in the quantum dot .The carrier density in the wetting layer and the quantum dot vary indifferent manner although the former lends to the later . Also thedynamics of carriers in the quantum dot shows varieties of dynamicsthat range from simple to complex one while the dynamics of carriersin the wetting layer are always simple .The polarized field related to the spin up and spin down cases sufferseffects in different manners due to the variations of these controlparameters . the intensities of both two polarized components behavioralso range from simple and end up chaotically.It appears that output ellipticity can be used as a new tool to assess the dynamical state of the laser wither it in stable , periodic , aperiodic or chaotic .The modulation of the injection current for both components or for either one led to new dynamics , the system was not able to show when it work autonomously although the model has five degrees of freedom .

خصائص نقل الالكترون العابر لانظمة نقاط كمية متعددة == Transient Electron Transport Properties of Multiple Quantum Dots Systems

Author name: حسه ابراهيم عسكر
Supervisor name: شاكر ابراهيم عيسى
General topic: Physics
Specific topic: Electronics Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: We presented a theoretical study for the electron transportcharacteristic, within a system of multi - quantum dots coupled tothe three metallic leads (right lead as an electron reservoir andtwo left leads as an electron source). We take into account twoarrangements for the scattering regions, one composed of twoquantum dots and the other is composed of four quantum dots.The study concentrated on the system parameters that has itseffect on the characteristic of the electron transfer process andhow these characteristic are affect by the number of the quantumdots in the scattering region in the both arrangements for thescattering regions.The theoretical formulism depends on the one - electron model.Where, we assigning a single effective level for each quantumdots and by taking the effects of the leads continuum levels intoaccount which are show within the wide band gap approximationto produce an imaginary quantity add to the energy levels of thequantum dots, representing the broadening in each effectivelevels.The related basic equation of motion for all the correspondinglevels eigen function probability amplitudes are solvedanalytically by using Laplace transform technique. We calculatethe occupation probability for the right lead, the instantaneouscurrent and the average instantaneous current that passes throughthe right lead.Our calculated results for the time - dependent dynamical electrontransfer offered a general features that takes into account thefollowing effects :  Energy levels position changes with respect to the leadFermi level. A suitable applied bais voltage on the right and left leads. Changes of the coupling amount among the quantum dots levels and in between them and the leads levels. Change of the leads temperatureThe characteristic of the time - dependent charge transport in the system are shown through the relation between the average instantaneous current passes the right lead and the applied baisvoltage, which we show as follow :  For the case of the scattering region arrangement that compose of two quantum dots it is an Ohmic behavior. For the case of the scattering region arrangement that compose of four quantum dots it is a non - linear (non - Ohmic) behavior.

قياس تركيز غاز الرادون والثورون في المنازل والمستشفيات والمراكز الصحية في محافظة البصرة العراق

Author name: حسام نجم عبود
Supervisor name: عيسى جاسم محمد الخليفة
General topic: Physics
Specific topic: Physics
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

دراسة تركيز البورون في مياه محافظة ذي قار وتربتها باستخدام طريقة الكارمين وجهاز اللوفي بوند == Study of Boron Concentration in Water of Thi - Qar Governorate and Soil Using Carmine Method and Lovibond Device

Author name: تغريد عبد الحسين شاكر
Supervisor name: ثائر منشد سلمان
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this study , we measured the boron concentration in the water and soil of Thi - Qar Governorates, where were collected (45) Sample Water also were collected (66) Sample soil from the districts of Thi - Qar and its environs province of southern Iraq , and we used that analogy chromatography Al carmine method (colorimetric carmine method) . we got the highest concentration of boron (0.801 ppm) in Garmat Bani Said area - Al Greej and the lowest value for the concentration of boron measured Al carmine manner of samples of clay in said Dhkeel - Al Ibrahim (2) area (0.368 ppm) . The concentration of boron has been in samples of mud rate in the province of Thi Qar was (0.614 ppm) also were measured exponent pH and ranged results between (7.208 - 8.368 ) and an average of (7.890) . As well as we used measurement chromatography Al carmine to measure the boron concentration in water samples way . We got the highest value for the concentration of boron water sample (1.057 mg / L) in Garmat Bani Said area - center (1) and the lowestvalue for the concentration of boron measured Al carmine way in water samples in Al Fuhood zone - Amayreh(1) (0.251 mg / L) . Boron concentration of water samples in the province of Thi Qar rat was (0.592 mg / L) .The boron concentration of water samples located within the normal allowable ratio globally but some are relatively high . As well as the measured pH and the results were pH ranging between (7.435 - 8.277) and an overall average of (7.885) .It was also some water samples to measure the soil by a device (Spectro Direct Lovibond) we got the highest value for the concentration of boron in the water samples (1.4 mg / L) in Al Fuhood zone - Amayreh area (3) , and the lowest value for the concentration of boron in Al Nasiriya - Al shuhada zone (1) (0.4 mg / L) . The soil samples were measured by a device (Spectro Direct Lovibond) has the highest value for the concentration of boron we got them in Al Tar - Al hareb area (1.1 ppm) and the lowest value for the concentration of boron in Al Tar - Al Ebrat (0.2 ppm) .

دور تذبذب الجسر في نقل الالكترون خلال نظام مانح - جسر - قابل == THE ROLE OF BRIDGE VIBRATION ON ELECTRON TRANSFER THROUGH DONOR - BRIDGE - ACCEPTOR SYSTEMS

Author name: بهاء علي طالب
Supervisor name: شاكر ابراهيم عيسى
General topic: Physics
Specific topic: Electronics Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: موضوع الرسالة هو دراسة تاثير اهتزاز الجسر وتكوين البولارون الصغير على عملية انتقال الشحنة بين المانح والقابل في الانظمة الجسرية الجزيئية. ولهذا الغرض تعاملنا مع اقتران الكترون - فونون قوي. واستخدمنا لهذا الغرض معادلات الحركة لسعات الدوال الكمية للنظام الجسري مع معادلة نمط الاهتزاز الديناميكي الكلاسيكي للجسر. بخصوص التعامل مع تقريب الحالة المستقرة والتقريب الساكن تم اعطاء صيغة لحساب طاقة البولارون وتقدير تاثيرها على عملية انتقال الشحنة الديناميكية بين المانح والقابل وبارامترات النظام التي تؤثر فيها. اذ لاحظنا ان الصفة العامة في منحنيات احتماليات الملء يكون اعتمادها على طاقة الجسر المؤثرة |∆(u)| اذ بزيادتها تقل احتمالية ملء مستوى الجسر ويقل عدد التذبذبات. ان وصفنا عملية منح البولارون بواسطة نظرية البولارون الصغير طورت لوصف الترابط القوي بين الالكترون والاهتزاز ضمن مفهوم البولارون الصغير، اي ان كل جسر يمكن ان يتذبذب (شبه) غير معتمد على تذبذب جيرانه من الجسور، او كل موقع جزيئي يتذبذب غير معتمد على تذبذب موقع جزيئي اخر. فضلنا المعالجة العددية في حل المعادلات والابتعاد عن الحل المستقر وتم حل معادلة النمط الاهتزاز الكلاسيكي حلا تحليليا بحيث يكون تاثير ترابط الكترون - فونون واضحا من خلال جعل مستوى طاقة الجسر معتمد على الزمن ويكون مسيطرا على احتمالية ملء مستوى الجسر. كما ان تاثير مستويات الطاقة المستمرة للقطبين المعدنيين على كل من مستويي المانح والقابل قد اخذ بنظر الاعتبار. وكان واضحا ان احتماليات ملء المستويات تضمحل بمرور الزمن وتتضاءل سعات التذبذب في المنحنيات، وان احتمالية ملء مستوى القابل هي الاكثر تاثرا بزيادة التعريض في المستويات. وايضا لاحظنا ارتباط تصرف احتمالية ملء مستوي الجسر مع تصرف احتمالية ملء مستوى المانح وذلك لان مستوى المانح هو مصدر الالكترون لتكوين البولارون مع الجسر.V وطبقت الصيغ الرياضية على عملية انتقال الشحنة خلال جزيئة الـ DNA. اذ تعاملنا مع نظام جسري ذي جزيئة اساس واحدة ونظام جسري ذي جزيئتين من الجزيئات المتسلسلة خطيا من جزيئات الحامض النووي الـDNA. اذ لاحظنا وجود اختلاف بسيط في احتماليات ملء المستويات بوجود القاعدة G/C او القاعدة T/A، او بوجود قاعدتين جزيئيتين، اذ لاحظنا ان التصرف الهام في منحنى احتماليات الملء هو عند زيادة ترابط الكترون - فونون نلاحظ تاثير تصرف منحنى احتمالية ملء مستوى مصدر الالكترون (المانح) على تصرف منحنى احتمالية ملء مستوى الجسر الاول (القاعدة الجزيئية الاولى) التي تكون على ترابط مع المانح وذلك لحدوث تشكيل البولارون الاول. بينما يكون تصرف منحنى احتمالية ملء مستوى الجسر الثاني مع تلك للجسر الاول غير متطابق بالتصرف وذلك بسبب ضعف اصرة الترابط بينهما. ويكون تصرف احتمالية ملء القاعدة الجزيئية الثانية مترابطا مع تصرف منحنى احتمالية ملء مستوي القابل وذلك لشدة الاصرة بين مستوياتهما. | The subject of thesis is the effect of bridge vibration and small polaron formation on the electron transfer (ET) in D - B - A system. For this purpose, we dealt with strong electron - phonon coupling. And we used for this purpose the equations of motion for quantum amplitudes and the equation of classical - dynamical vibration mode of the bridge system. For the case of steady state approximation and static approximation we gave a formula to calculate the polaron energy and estimate its effect on the dynamical ET between the donor and accepter and the parameters involved. where we note that the general feature of the occupation probability curves are depend on the bridge effective energy |∆(u)|, when it increases, the occupation probability and the number of vibration decreases. The description of polaron hopping by the theory of small polaron was developed to describe the strong coupling between the electron and vibration within the concept of small polaron, i.e. every bridge vibrates (semi) independently from neighboring bridges. we preferred the numerical treatment in solving the equations and get away from the static solution, we solved the equation of classical - vibrational mode analytically such that the effect of electron - phonon coupling became obvious by putting the bridge state in a time - dependent form, and controlling the occupation probabilities of the bridge. The effects of the continuous energy levels of the metlic electrodes on the donor and acceptor states is taken into account. And it was obvious that the occupation probabilities of levels vanish in time and so as the amplitudes of vibration, and the most obvious effect of continuous levels of electrodes is on acceptor state, also we note that the behavior of the bridge state occupation probability is related with the occupation probability behavior of the donor state, since the donor state is the source of electron for polaron formation in bridge site.We applied the mathematical formulae on charge transfer through DNA molecule. Where we dealt with bridge system as a single base molecule or two liner consecutive molecules bases of DNA molecules. Where we note there is a small difference in occupation probabilities of G/C base or T/A base or between that of two bases. The important feature in occupation probabilities curves is that when electron - phonon coupling increases, we note that the effect of the occupation probability behavior curve for donor state on the occupation probability behavior curve of the first bridge which is connected with the donor because of the formation of the first polaron, while the behavior of the occupation probability of the second bridge and that of the first bridge is not coincide because of the weak interaction between them, but the occupation probability behavior of the second bridge state is related with that of the acceptor because of the strong interaction between them

دراسة نظرية لبعض العوامل المؤثرة في سلوك دالة الموجة المستخدمة في تكثيف بوز اينشتاين

Author name: ايمان عبد الرضا موحي المالكي
Supervisor name: نوري حسين نور الهاشمي
General topic: Physics
Specific topic: Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study entails some of the theoretical characteristics of Bose - Einstein intensification of the ideal gas properties, since the wave function behavior has been studied and analysed according to the effect three types of external potentials trapping that are used in the production Bose - Einstein condensation which are (harmonic potential, optical lattice potential , and double well potential) that have been used as individual or overlapping potentials, and the value of the non - linear has been confirmed according to fixed values in the whole study in order to the study the effect of the other elements. The effect anisotropy AL has been studied according to the behavior of the wave function and the distribution potential where the harmonic potential has individually at first, than it has been overlapped with the optical lattice potential in order to study this factor. Furthermore,the effect of this factor has been explained due to the distribution potential, function wave and relation type between the factor itself, chemical potential and energy. Additionally, we have used the intertwined harmonic potential with the optical lattice to study the effect of optical lattice potential factor q after confirming the harmonic potential AL at certain values, with exposition of it's effect the wave function behavior and distribution of potential with explanation to the nature of the relation between the chemical potential and energy with this factor. In addition to that, we have studied the influence of the center of double well potential coefficient A on the nature and behavior of the wave function and potential, and the nature of the relationship between this factor and chemical potential and energy. Noting the effect of these factors on the behavior of the wave function and the distribution of potential, therefore it has effect in Bose - Einstein condensation production.

خصائص نقل الالكترون لكيوبت مقترن بترانزستور الالكترون المفرد == The Electron Transport Properties of Qubit Coupled with SET

Author name: ايات طاهر ابراهيم
Supervisor name: جنان مجيد المخ
General topic: Physics
Specific topic: Electronics Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: In this work, the dynamics of electron transport through a system consists of qubit (two coupled quantum dots) electrostatically coupled with single - electron transistor (quantum dot coupled to leads). The time - dependent Hamiltonian, that is used to describe the system energy, includes the electrostatic coupling between the qubit and the single - electron transistor. The time - dependent equations of motion for all the creation and annihilation operators are expressed in Heisenberg representation to derive the differential equations of motion for the quantum dots occupation numbers of the qubit and the single electron transistor, the related equations of motion for the correlation functions and also the formula that calculates the current which tunnels from the left lead to the single - electron transistor quantum dot. The system of equations of motion are treated by using the wide band approximation, and are solved numerically by using six order Runge - Kutta method, where the error is calculated at each step of time. By getting use of the system of differential equations solutions, the qubit and the single - electron transistor quantum dots occupation numbers are calculated as a function of time, in addition to the current that tunnels from the left lead to the single electron transistor quantum dot as a function of time.The main goal of this study in to investigate the effect of the parameters that related to the qubit and the single - electron transistor and their role in determing the electron transport process features through the whole system. These parameters, that are related to the initialization, manipulation and measurement processes, are the qubit and the single electron transistor quantum dots energy levels, the coupling strength between the qubit quantum dots, the electrostatic coupling between thequbit and the single electron transistor in addition to the coupling strength between the single - electron transistor quantum dot and the leads as well as the leads properties such as tempreture and band width. The effects of quantum dots energy levels tuning and the symmetry of the coupling with leads on the electron transport through the system are also investigated.Our study highlights four important physical features that related to the initialization, manipulation and measurement processes, these are : 1. The occupation numbers of the quantum dots of the qubit and the single electron transistor and the current of SET at the final time of measurement.2. The charge accumulation on the qubit quantum dots, at the final time of measurement, on the far - removed quantum dot and the nearest one to the single - electron transistor, that can be controlled by appling bias voltage and gate voltage.3. The determination of the time - current dependence if it follows the dependence of the far - removed or the nearest qubit quantum dot occupation number on time.4. The role of qubit energy levels tunning and the asymmetric coupling with the leads in determining the suitable initialization and manipulation processes get the quantum measurement with high quantum efficiency.

قياس تراكيز الرادون للمستشفيات ,المراكز الصحية والمنازل وقياس البورون في مياه محافظة ذي قار - العراق == Measurements of Radon in Hospitals, Health Centers and Dwelling as well as Boron Measurements in Water of Thiqar Governorate(Iraq)

Author name: احمد عباس محمد
Supervisor name: عيسى جاسم محمد الخليفة
General topic: Physics
Degree: Doctorate
Language: English
University location: Basrah
First pages:
Abstract: العمل الحالي يتضمن ثلاث دراسات اجريت جميعها في محافظة ذي قار جنوب العراق, حيث استخدم 976 نموذج فيها.الدراسة الاولى استخدمت 625 نموذج من كاشف الاثر LR 115 Type II جمعت من 40 منزل في كل قضاء , حيث تم قياس مستويات الرادون وخطر الاصابة بسرطان الرئة من خلال الطريقة السلبية . النتائج اظهرة مستويات مرتفعه وخصوصا في المطبخ مقارنتا مع الاماكن الاخرى وذلك بسبب غاز الطبخ والمياه المستعملة. ان المعدل للجرعة المكافئة السنوية للمطبخ تتغير من 2.316±0.172 في الناصرية الى0.759±0.0348 في سوق الشيوخ .اما الدراسة الثانية فقد استخدمت 186 نموذج من كاشف الاثر LR 115 Type IIجمعت من 57 مستشفى ومركز صحي , حيث استعملت الطريقة اعلاه لقياس مستويات الرادون وخطر الاصابة بسرطان الرئة هناك.لقد اظهرت النتائج ان مستويات الرادون في المستشفيات اعلى من نظيرتها في المراكز الصحية. ان اعلى معدل للجرعة المكافئة السنوية للمراكز الصحية 2.504±0.114 mSv/y بينما للمستشفيات3.150±0.091 mSv/y. في كلى الدراستين اعلاه وجد ان اغلب الجرعات الاشعاعية , ليست اعلى من المعدل العالمي 2.4 mSv/y , ولذلك فانها لا تشكل خطرا جديا على المتواجدين.ان الدراسة الثالثة هدفها قياس تراكيز البورون في مياه محافظة ذي قار , حيث ان امدادات المياه تاتي من مصدرين رئيسين وهما نهري دجلة والفرات, حيث جمع 165 نموذج من 55 موقع لثلاثة انواع من المياه (مياه نهر, مياه اسالة , مياه شرب) واستخدمت الطريقة اللونية لقياس تركيز البورون في عينات الماء. ان اعلى التراكيز سجلت لمياه الانهر في قضاء الناصرية 1.729 mg/l , ولمياه الاسالة سجلت في قضاء الجبايش 0.3 mg/l , اما مياه الشرب فسجلت في قضاء الجبايش 0.286mg/l ,حيث النتائج اظهرت كذلك ان نهر الفرات اكثر تلوثا بعنصر البورون وذلك بسبب ان المناطق ذات الكثافة السكانية العالية ,المصانع والمنشات الكبيرة تقع على ضفافه , حيث ان معدل لتراكيز البورون لنهر دجلة 0.244 mg/l , بينما لنهر الفرات 0.794 mg/l . الدراسة الحالية اظهرت ان تراكيز البورون لنهر دجلة ليست اعلى من المعدل العالمي (اقل من 0.5 mg/l) . بشكل عام نتائج نهر الفرات اعلى من المعدل العالمي 0.5 mg/l , ولكنها تبقى ضمن المستويات المنخفضة لتراكيز البورون , حيث حدد الاتحاد الاوربي المستويات المقبولة ب 1.0 mg/L عام 1998 , اما نيوزيلندا فحددته ب 1.4 mg/L. | The present work included three studies carried out in Thiqar Governorate southern of Iraq, where 976 samples has been used .The first study used 625 samples of LR 115 Type II dosimeters where collected from 40 dwelling in each distract, Passive method is used to measure the radon indoor levels and lung cancer risk. The results show higher indoor radon levels and radon effective dose especially in kitchen as compared to other locations. High values of radon activity may be due to use of water and cooking gas in kitchen.The average annual effective dose for kitchen varying from 2.316±0.172 mSv/y recorded in Al Nasiriya district to 0.759±0.0348 mSv/y recorded in Suq AlShyouk district.The second study used 186 samples of LR 115 Type II dosimeters collected from 57 Hospitals and Health centers, also Passive method is used to measure the radon indoor levels and lung cancer risk. The results show that the calculated indoor radon levels and radon effective dose in Hospitals are higher than that in Health Centers.The maximum average annual effective dose in Health Centers is 2.504±0.114 mSv/y , while for Hospitals is 3.150±0.091 mSv/y.In the two studies most of the radiation dose are not higher than the world wide average back ground dose of 2.4 mSv/y and hence they does not pose any serious threat to the occupants.The third study aims is to measure the concentration of boron in waters of Thiqar Governorate (southern of Iraq), the water is supplied from two major sources (Tigris and Euphrates Rivers). 165 samples collected from 55 location for three types of water (rivers water, Tab water, drink water). Colorimetric curcumin method is used to measure the boron concentration in the water samples. The maximum Boron concentrations in Thiqar Governorate for River water (surface water) (1.729 mg/l) in Nasiriya district, Tab water(0.3 mg/l) in Chibiesh district and Drink water (0.286mg/l) in Chibiesh district. The results shown that Euphrates river is more polluted with boron element, because its pass in highly occupied region and meager factories ,facilities lay on it, where Tigris average value 0.244 mg/l,while Euphrates average value 0.794 mg/l.The present work shows most of the boron concentration in Tigris river aren't higher than the world wide back ground <0.5 mg/l. In general Euphrates river results are higher than the world wide back ground, but it's still in the low concentration levels for boron, where the European Union established a value of 1.0 mg/L for boron in 1998 for the quality of water intended for human consumption, also New Zealand has established a drinking water standard for boron of 1.4 mg/L

اللااستقراريات والفوضى في الدوائر الالكترونية

Author name: ابو طالب يوسف عباس عبد الكريم الشامي
Supervisor name: نوري حسين نور الهاشمي | فرات احمد مهدي السيمري
General topic: Physics
Specific topic: Electronics Physics
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة حيوية وتشخيص جزيئي لداء الاكياس العدرية في المضائف الوسطية اعتمادا على تحليل تتابع جينات rDNA - ITS1 وmtCOX1 في محافظة ذي قار == Biological Study and Molecular Identification on Hydatidosis in Intermediate Hosts Depending on Sequence Analysis for rDNA - ITS1 and mtCOX1 Genes in Thi - Qar Province

Author name: وسام جاسم حنش
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was carried out in Thi - Qar province during the period from beginning of January until the end of December 2015 to investigate the prevalence of infection with hydatidosis in human and other intermediate hosts. The molecular techniques was used to detect the strain of Echinococcus granulosus. The present study diagnosed 58 infection with hydatidosis in different organs such as liver, lungs, kidney and spleen of surgically treated patients in AL - Hussein Imam Teaching Hospital inNassiriyah city, centre of Thi - Qar province. The total number of hydatid cysts was 72 cyst in different organs with total intensity of infection 1.24. The females recorded highest percentage of infection 67.24% than males 32.76 %, and the age group 21 - 30 year showed highest percentage of infection 24.14 % . The lowest percentage recorded in age group more than 60 year 5.17 %.The results of study showed that right lobe of liver was more affected 58.62 % than left lobe 13.79 %. The percentage of left lung infection was 10.34 % while right lung 8.62 %. The total average of fertile cysts in infected organs was 81.82 % and sterile cysts 18.18 %. The highest percentage of infection was recorded in house women 87.18 %. The number of recorded cases in Nassiriyah district was 21 case with percentage of infection 36.21 %.During the same period a total of 1303, 1287, 405, 90, and 294 head from sheep, cattle, buffaloes, camels and goats were examined respectively.The percentage of infection was 7.83 %, 7.69 %, 3.46 % and 2.22 % in sheep, cattle, buffaloes and camels respectively, and no infection was recorded in goats. The intensity of infection was 4.07, 3.27, 1.64 and 2 in sheep, cattle, buffaloes and camels respectively. The results of statistical analysis showed no significant differences (P????0.05) in percentage and intensity of infection.The percentage infection of liver, lungs and both together in sheep was 5.45 %, 1.23 % and 1.15 % respectively, and there was no significant differences in percentage of infection but intensity of infection differed significantly(P????0.05). The percentage of liver, lungs, both together and spleen infection in cattle were 3.11 %, 2.95 % , 1.55 % and 0.08 % respectively while intensity of infection in liver, lungs, both together and spleen was 2.5, 2.18, 7 and 1 respectively. The percentage of liver, lungs and both together infection in buffaloes were 2.22 %, 0.99 % and 0.25 % respectively while intensity of infection in liver, lungs and both together was 1.89, 1 and 2 respectively. No significant differences in percentage and intensity of infection in camels andboth percentage and intensity of infection in liver and lungs were 1.11% and 2 respectively.High significant differences was recorded between percentage infection in males and females of animals. The percentage of infection in females were 15.87 %, 13.42 %, 6.99 % and 3.45 % in sheep, cattle, buffaloes and camels respectively. Highest percentage of infection were 0.72 %, 0.18 % and 0.46 % in males of sheep, cattle and buffaloes respectively. No infection were recorded in males of camels.T he age was effect on percentage of infection and big aging groups recorded in sheep, cattle, buffaloes and camels. The present study recorded significant differences in percentage of fertile hydatid cysts in liver and lungs of animals and highest of percentage of fertility recorded in liver and lungs of sheep 84.21 % and 79.24 % respectively and lower percentage of fertility was in liver of buffaloes 11.11% and liver of cattle 14.70 %. The lower percentage of sterile hydatid cysts was in liver and lungs of sheep 15.79 % and 20.75 % respectively and highest percentage recorded in liver of buffaloes 88.88 % and spleen of cattle 100 %.S t a tistical analysis using of ANOVAs test showed no significant differences in total length, length of blade and width for large and small protoscolices hooks of hydatid cysts isolated from liver and lungs of sheep, human, cattle buffaloes, camels and hooks of sheep which is showed genetic variations (new strain).The PCR - RFLP technique was used in identification of E.granulosus strains infecting human and intermediate hosts in Thi - Qar province. The molecular study is carried out on 75 hydatid cysts collected from human, sheep, cattle, buffaloes and camels. The DNA is extracted from protoscolices of hydatid cysts isolated from infected organs. PCR - RFLP technique showed that sheep strain G1 of E.granulosus is predominant in Thi - Qar province depending on amplification of rDNA - ITS1 gene. The PCR products in all DNA samples showed 1000 bp approximately. Alu1 endonuclease with rDNA - ITS1 demonstrated two bands 800 bp and 200 bp approximately, while Rsa1 endonuclease showed two bands 655 bp and 345 bp approximately which is similar to sheep strain genotype 1.Sequence analysis for rDNA - ITS1 gene showed the presence of sheepstrain genotype 1 which is similar 100 % to sheep strain in gene bank exceptone isolate (sheep liver) was differs from other strains in position ofnucleotide sequence. It was similar 99 % for sequence of sheep strain. Thedata base of this new strain was recorded in gene bank for DNA sequence National Center for Biotechnology Information (NCBI) and European Nucleotide Archive (ENA) for the first time globally under the name Echinococcus granulosus strain WH1AW2 (GenBank : LT547814). Polymerase chain reaction technique and sequence analysis of mtCOX1 gene showed the presence of sheep strain G1 of human, sheep, cattle, buffaloes and camels. The sequence analysis showed the genetic variation in mtCOX1 gene for hydatid cyst in infected sheep represented by point mutation type transition which caused change Adenine to Guanine, and sequence data base of the sample recorded for first time in NCBI and ENA (GenBank : LC203589). Buffaloes strain G3 isolated from human lung recorded for first time in Thi - Qar province depending on comparison of samples sequences with source strains recorded in gene bank (NCBI) using "BLAST". The size of bands for mtCOX1 gene was 450 bp in all samples

دراسة فعالية بعض مستخلصات الطحلب الاخضر Enteromorpha intestinalis (L.,1820 على الاكياس العذرية للمشوكة الحبيبية Echinococcus granulosus (Batsch,1786 وكمضادات لخط الخلايا السرطانية نوع HeLa == Study of the activity of some of the green alga Enteromorpha intestinalis (L.,1820) extracts on Hydatid cysts of Echinococcus granulosus (Batsch,1786) and as anticancer cells type HeLa

Author name: غزوان طالب نوري الجابر
Supervisor name: صبيح هليل المياح | احمد محسن عذبي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted in order to evaluate the activity of green alga Enteromorpha intestinalis extracts (methanol and hexane extracts) on growth and development of hydatid cysts of Echinococcus granulosus in vitro and in vivo by using mice type Mus musculus Balb\c that in comparing with albendazole drug. Anti - cancer activity was also evaluated in vitro for both algal extracts by using HeLa cells. Chemical compositions of algal extracts were analyzed by using Gas chromatography - mass spectroscopy (Gc - mass)technique . The hydatid cysts were collected from livers and lungs of infected sheep which slaughtered in Basrah abattoir. While, The algae specimens were collected from Abu Sokhir marshland area /north of Basra. The results revealed that E.intestinalis chemical compounds are carbohydrate , alkaloids, phenols , saponins , triterpenes and sterols compounds. In vitro study showed that the 1 mg / mouse weight of albendazole drug was able to reduce the percentage of protoscolices activity to zero after the third day of treatment. While, the dose 6 98 mg / kg of methanol extract inhibited all protoscolices after five days of treatment, followed by the dose 804 mg / kg of hexan extract that inhibited all protoscolices after six days of treatment.In vivo study revealed that the methanol extract (698 mg / kg) treatment was the best in reducing the weights of organs significantly in comparison with the positive control group it was 2.192 , 0.192 , 0.434 and 0.282 g of liver, spleen, lung and kidney respectively. The methanol extract (698 mg / kg) treatment lowered the average number of hydatid cysts (2.6) more than other extracts treatments , then followed by hexane extract (804 mg / kg) treatment it was 2.8 cyst .This study has identified that the liver enzymes Aspartate transaminase (ALT) and Alanine transaminase (AST) values in the serum of positive control group were higher significantly than negative control group the value of the enzyme ALT was 79 IU / l and the value of the enzyme AST was 118.4 IU / l.All methanolic extracts groups showed a preference in enzyme's values compared with other treatments groups , the value of ALT enzyme of 698 , 688 and 678 mg / kg of methanol extract groups were 46.8 , 45.8 and 44.6 IU / l respectively, while all hexane extracts groups showed a preference in AST enzyme values compared to the other treatments groups, the value of AST enzyme of 804 , 794 and 784 mg / kg of hexane extract groups were 73.6 , 70.2 and 70.4 IU / l respectively. Found a significant increase in the number of white blood cells WBCs in a positive control group ( 4.6 × 013μ), compared with a negative control group and the other groups, While it has been found significant decrease in the Hb (5..8deciliter) and Packed cell volume (PCV) (31..%) values in positive control group as compared with control negative group. The study found that the number of white blood cells WBCs n the methanolic extract group (678 mg / kg) was 4.06 × 103 microliter was as same as in the negative control group, In contrast, hexane extract group (804 mg/ kg) has did not significant difference with negative control group in the Hb and PCV were 11.5 g / dl and 36.8% respectively .Histological study confirmed that there are the histological changes in positive control group included congestion , bleeding , degeneration , infiltration of inflammatory cells and hyperplasia. Whereas the histological changes in group treated with albendazole were bleeding, infiltration of inflammatory cells , atrophy of the glomerulus and fibrosis in some areas . In algal extracts groups the histological changes were infiltration of inflammatory cells, bleeding and congestion.The Gc mass results revealed that the ethanol extract contains loliolide , ethyl stearate , palmitic Acid , ethyl palmitate , phytol , ethyl oleate , ethylhexyladipate and squalene compounds. While, hexane extract has N, Ndimethyltetradecylamine , diisobutyl phthalate , palmitic Acid , ethyl palmitate and ethylhexyladipate compounds.Anti - cancer study showed the algal extracts have an inhibition activity against cancer cells, The half maximal inhibitory concentration (IC50) value of the methanol extract was 79.08 μ/ ml, While, the IC50 for hexane extract was 156.3 μ/ ml.

دراسة تشريحية تصنيفية لبعض من نباتات العائلة السوسبية Euphorbiaceae في العراق == Anatomical and Taxomanical study for some plant of Euphorbiaceae family in IRAQ

Author name: امل علي ياسين الحسن
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted to investigate several taxonomic aspects which comprised the micro - morphological studies of epidermal layer of leaves, pollen grains, fruits and seeds.In addition to that, the anatomical and molecular studies for 12 species of Euphorbiaceae family which belonged to five genera AndrachneL. , Chrozophora L., Euphorbia L, Mercurialis L. and RicinusL., were performed. One representative species for each genuswas selected; in exception 8 species were chosen for the genus Euphorbia..The current results confirmed that the anatomical characters showed valuable taxonomic traits which can evidently be used to distinguish between species. The most prominent trait was mesophylla tissue in leaves which were observed in three categories;1 - Bifacial leaves : - which found in C.tinctoria, Euphorbia sp ., E. milii, E.annua and R.comminus 2 - Uni - facial (heterogenus) leaves : - this category of the mesophyll was detected in two species only A.telephioides and E.prostrata3 - Uni - facial (homogenus) leaves : - this category were noticed in five species; E.denticulata ,E.helioscopia ,E,macrocarpa , E.microsphaeia and E.peplus The second important comparative anatomical character was the Mid rib, which are in four types with several shapes from which discrimination between the species can be made : - 1. Convex - concave mid rib : - that was showed in E.helioscopia, E.macrocarpa, E.milii and M.annua.2. Convex - convex : - This type was found in three species C.tinctoria , Euphorbia sp.and R. comminus3. Flat - Convex : - This type of mid rib was observed in A.telephioides E.microsphaera, E.peplus and E.prostrata4. Flat - Flat : - This type was observed in only one species E.denticulata In stem, the most prominent parts of taxonomic evidence were pith and vascular bundles.In according to the pith, species can be divided into hollow stem (without pith) and solid stem (containing medulla good configuration). Hollow stem was clear in the stems of A.telephioides , E.microsphaera , E.prostrata and M.annua. While, the solid stem wasseen in the remaining species. Vascular bundles were existed in two types; that's connected vascular bundles that's characterized the hollow stems, and separate vascular bundles. In most of the species with the exception of M. annua, stem with scatteredvascular bundles were in cortex As well as, petiole which have features that a clear taxonomy values .either by outline of petiole or its vascular arc.Micro - morphological studies of epidermal layer of leaves showed very important taxonomical value. This layer of leaves reveals variation in shape of ordinary epidermal cell and nature of anticline cell wall, stomata complexes and Epicuticlular wax crystals.Micro - morphological of pollen was taxonomic evidence as well. It can be considered to divide the species of Euphorbiaceae family into two groups based on the number of germination apertures 1 - Hexocorporate : - this type was confined to C.tinctoria2 - Tricolporate all species studied remainingThe sculpture of pollen wall provided beneficial information invested in the species identification.Both seeds and fruits showed a distinctive role in species identification. Micromorophological study of seeds for species which belong to six genera Andrachne , Chrozophora, Euphorbia, Mercurialis , Phyllanthus and Ricinus were achieved by using Scanning Electron Microscope(SEM). The study concentrated on micro - morphological aspect of Caruncle, seed surface ornamentation and the shape of epidermial cell wall. The result revealed that most studied species were with carunclated seeds ,but some specieswere with ecarunclated seeds as in A.telephioides L. ,C. tinctoria( L) Raf , E.prostrata Atin and P. maderaspatensis L Seed surface ornamentation characteristics showed a significant taxonomical value.Furthermore, current result showed that there are a highly variation in shape, size and color of the seeds walls. With respect to the fruits characteristics, which showed variations in number of mercarpi, shape, size, color and surface ornamentation that can be adopted in the separation of the species studied Molecular study provided significant support for phenotypic and anatomical classification and support genetic convergence between the species in Euphorbiacea family. The genus Euphorbia species showed a significant genetically convergence and participated in predecessors monophyletic. However, the genera in Euphorbiacea family under study probably originated and evolved from different ancestors’ polyphyletic.

دراسة تصنيفية وحياتية للديدان الخيطية عائلة Tetrameridae Travassos, 1914 المتطفلة على بعض انواع الطيور في هور السناف محافظة ذي قار == Taxonomic and Biological Studies of Nematodes of the Family Tetrameridae Travassos, 1914 in the Some Birds in Sinaph marsh Thi - Qar province

Author name: نهى جبار عبد الركابي
Supervisor name: باسم ھاشم عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 401 represented by 128 birds of Himantopus himantopus which belong to the family Recurvirostridae , 93 birds of Chettusia leucura which belong to the family charadriidae and 180 birds of Sturnus vulgaris which belong to the family sturnidae at the period from April 2014 to march 2015 in Al - Sanaf marsh , Suq - Al - Shuyukh city southern of the Thi - Qar province,for searching the nematodes which belong to the family Tetrameridae. Four species of nematodes, which belong to the family Tetrameridae, were isolated in the current study. They were T.nouveli, T.dubia, T.spinosa and Microtetrameres inermis. The H. himantopus was infected with the nematode T.nouveli and the prevalence of infection was 25.8% , bird.Furthermore, the Chettusia leucura was infected, the mean intensity was 3.3 worm in each infected birds. The nematode T.dubia was isolated from H. himantopus with the isolated from the Ch. Leucura mean intensity was 1.7.The nematode T.spinosa was isolated from H. himantopus the the mean intensity of infection was 2.3 and it was isolated from the Ch. leucura , the prevalence of infection 4.3% and the mean intensity was 1.8 worm in each infected worm.The record of these three nematode is considering the first record in Iraq.Microtetrameres inermis was isolated from the Sturnus vulgaris, the each infected birds.Himantopus himantopus from April 2014 to March of 2015.This bird was infected with three species from family Tetrameridae, The seasonal variation study of infection prevalence of Tetrameres spp.in, H. himantopus that there was II Summarycomplete seasonal cycle for nematodes T.nouveli, T.dubia , and T.spinosa .The nematode T.nouveli had hightest infection prevalence in summer the decrease in autumn, winter and spring while the infection prevalence of nematode T.dubia was hight in summer and autumn then decrease in winter and spring. The infection prevalence of nematode T.spinosa was generally low with little increase in autumn .The results of the current of the Ch. leucura indicated that this bird was infected with three species which nematodes T.nouveli, T.dubia and T.spinosa and they had seasonal appearance . There was not any significant effect of host sex on prevalence of infection, and it showed decreased in prevalence of infection when the bird's weight was increased.The study of lifecycle of nematodes : T.nouveli ,T.dubia and M. inermis revealed obtainment the larval stages after success the infection experimental of the grasshopper Schistocerca gregaria for the first time the three isolated nematodes from family Tetrameridae in this study which it was T.nouveli , T. dubia and M.inermis and was included the lifecycle of the three species . The differences were including the period of time to produce and developing the larval stages in addition to the differences in their shapes, it was revealed that were clear differences in the structure and their shape of the tip the posterior end of the third larval stage. The end of the third larval stage of the nematode T. nouveli had three caudal papilla and the posterior end of nematode T.dubia had five caudal papilla while the end of the third larval stage of M.inermis did not have any caudal papilla , so we can distinguish these nematode larval stage depended on the prevalence this character in their third larval stage.The experimental infection of the Columba livia domestica with nematode M.inermis was successful.While the experimental infection to the Columba livia domestica with nematode T.nouveli ,T.dubia .was failed The experimental infection of the Gallus domestica with nematodes of Tetrameridae T.nouveli, T.dubia and M.inermis was failed too.

دراسة تشخيصية وحياتية لعلقة المياه العذبة Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea في اهوار السويب من محافظة البصرة جنوب العراق == Identification and Biological study of the Freshwater Leech Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea) in AL - Swaib Marshes From Basrah province south of Iraq

Author name: ازهر محمد غالي الخزعلي
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The species Dina punctata is recorded for the first time in Iraq (from Al - Swaib marshes). The identification was conducted morphologicaly and molecularly characteristics. The morphological features summarized as : - - The species D. punctata has vermiform body compressed ventrally, ranging in length from 55 - 60 mm and in diameter from 0.5 - 2 mm. The individuals have a large ability for extening. The pointed anterior end carries small anterior sucker which resembles lips surrounding the mouth opining, while the rounded posterior end carries large rounded posterior sucker. - This species is characterized by heterogeneous features such as the color of the body which changes from blackish gray for Adults to dark brown for Juveniles, as well as the number and arrangement of eyes which is in general four pairs arranged as reverse U shape located on the dorsal anterior edge. The first two pairs of eyes are located on the middle line of the body while the third and the fourth pairs are each located on one side of the body behind the anterior pairs. - The body consists of 34 segments, each of them is divided to five secondary annuli. The fifth annuli of each segment is divided into two subsections, thus the main segment becomes consist of six annuli. - The male and female gonopores are open on the ventral surface of the 12th segment, separated from each other by two annuli, the male gonopore is usually larger and clearer and located forward in the middle of the groove which is separate the annuli b2 and a2 respectively. The female gonopore is smaller and located in the back of the male gonopore in the middle of the groove which is separate the b5 and b6 annuli respectively.Because of the instability in some morphological features, the species D.punctata also identified in the present study according to genetic features.During the genetic study, the region of internal transcription space (ITS2) in DNA was isolated from leech specimens by using special primers for region internal transcription space (ITS2), after isolation, the ITS2 genes are amplified.The results of polymerase chain reaction of DNA were obtained of single bands of 580 base pairs molecular weight for all degrees of hybridization. The sequence of each sample after detecting was entered in the database of National Center Biological Information (NCBI) and the result showed that the sequences of all samples are belonging to the species D. punctata. In the field study, monthly samples of leeches D. punctata was collected from the marshes of Al - Swaib on the eastern side of Shatt Al - Arab river inQurna northern Basrah Province during the period from January 2015 to December 2015. A total of 235 individuals and 623 Cocoon were collected during the study period. The highest number was 58 recorded for individuals during May 2015, while being 335 recorded for cocoons during February 2015. On the other hand there is no occurrence recorded during July - August for individuals, and June - November for cocoons.The study investigates the water temperature, salinity and abundance of individuals and cocoons. The statistical analysis showed negative correlation between temperature and abundance of both individuals and cocoons, while the correlation of salinity was slightly positive with abundance of individuals and cocoons.The study of population sizes structure showed that the size class 40 - 50 mm has the highest frequency during the study period compared with the other size classes. On the other hand, the highest percentage of frequency was 66.7%, which also recorded for the size class 40 - 50 mm.Laboratory experiments showed that the members of a species D.punctata able to tolerate saline concentrations between 2 - 6 psu the survival rate 100%, Also, these worms (D. punctata) feed by different ways, it can be considered Predatory Macrophagous and Scavengers, its food materials consist of invertebrate animals such as Oligochaetes, Lumbriculids, Chironomid larvae; fleshy body of some aquatic snails; and on dead fishes. In the laboratory, the leeches fed well on flesh and livers of chicken, sheeps and caws, The laboratory also studied, Three different types of food effect (chicken liver, zooplankton, and animal feed) on the growth of young leech for a period of six weeks.From the field and laboratory observations, the specie D. punctata reproduces sexually by cross fertilization. The male and female gonopores are easily observed in adults which ranging in length from 45 - 60 mm. as well as the sperm sacs on the mid ventral surface of the body during the reproduction season, which extend in our study during two periods : January - May and December 2015. The sexual reproduction processes were studded in detail and the embryonic development in cocoons was divided into five hypothetical stages according to morphological and anatomical changes and these stages are : - 1 - Depositing Cocoon Stage.2 - Primary Cleavage stage.3 - Development and extension.4 - Differentiation of regions stage.5 - Complement and release stage. The histological structure of the body wall, suckers, reproductive organs and digestive tract of adult leeches were investigated. The body wall consists of thick layers of circular, longitudinal and oblique muscles.The structures of the male reproductive system such as genital atrium, sperm sacs and testis were observed. There are two lateral blood sinuses around the mouth cavity in addition to many nerve cells. The posterior sucker is muscular, and the muscle cells are as a dense net in the peripheral part, while are as a loose cells in the middle what give it a sponge appearance, in addition to primitive glomerular cells, nerve cells and mucus cells

دور مستارجات حشرتي الصرصر الامريكي Periplaneta mericana والبعوض Culex pipiens على فرط التحسس من النوع الاول وعلاقتها مع بعض الائل مستضدات معقد التوافق النسيجي من الصنف الثاني

Author name: هدى كاظم كريم الموسوي
Supervisor name: فوزية علي عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study aimed to verify the distribution of Periplaneta americana and Culex pipiens allergy and its association with some HLA class II alleles in asthmatic and allergic patients. 96 healthy (control ) and 96 suspected allergic individuals from the same geographical region ,paired by sex and age were included in this study. Enzyme Linked Immunosorbent Assay test (ELISA) was used to estimate the total and specific IgE antibodies in the sera of studied individuals. The detection of the HLA - DQB1*0602, HLADQB1* 0604 and HLA - DRB1*12 was performed by Polymerase Chain Reaction (PCR).The results of total IgE based ELISA which was conducted on 96 suspected allergic and24 healthy (control ) individuals revealed that there was extremely significant statistical association (P<0.0001) for total IgE concentration with being allergic or non allergic status of individuals. The overall frequency of higher total IgE antibody concentration ( IgE>200IU/ml) was observed in 72.9% of allergic patients and lower serum total IgE levels (IgE<200IU/ml) were associated with 87.5% of healthy individuals. According to the specific IgE based ELISA results the effect of allergic patients age on the IgE seropositivity against cockroach allergens was not considered to be significant(p>0.05),but the effect of sex was statistically significant)P<0.05(.There was no significant effect( p>0.05)for patients age and sex on the distribution of IgE antibodies against mosquito allergens. Out of 96 allergic patients, 59 (61.5%)and 62(64.6%)showed positive IgE antibody response against cockroach and mosquito allergens respectively. Concerning the PCR results the overall frequency of HLA - DQB1*0602 and HLA - DQB1*0604 alleles was %35.6 and %15.3 of cockroach allergic patients respectively and the HLA - DRB1*12 allele was not observed in these patients..Out of 62 mosquito allergic patients, 8(%12.9)showed HLA - DQB1 * 0602 positive results while HLA - DQB1*0604 allele was observed in 4(%6.5)patients and the HLA - DRB1*12 allele was not observed in these patients.The difference in the genotypig results was extremely highly significant(χ2 : 0.030;17.486; degrees of freedom(DF) : 3;p - value=0.00056136) between cockroach and mosquito allergic patients .The association between the presence HLA - DQB1 *0602 , HLA - DQB1 * 0604 and HLA - DRB1*12 and total IgE based ELISA results in allergic patients and healthy individuals revealed that the percentage of HLA - DQB1 * 0602, HLA - DQB1 * 0604 and HLA - DRB1*12 alleles presence in patients having total IgE lower total IgE )IgE<200 IU/ml) was higher than that of high )IgE>200 IU/ml) total IgE patients where HLA - DQB1*0602 appeared in high percentage(69.2%) followed by percentage (15.4 %( of HLA - DQB1* 0604 presence. . The relationship of HLA - DQB1and HLADRB1 alleles with the results of total IgE based ELISA was statistically significant in the least(χ2 : 60.756 and the degree of freedom (DF) : 5;p= P<0.00001) and greatest(χ2 : 11.806 ; the degree of freedom (DF) : 5 ; p = 0.037544) than 200 IU / ml, values .Depending on the association between the presence of HLADQB1* 0602 ,HLA - DQB1*0604 and HLA - DRB1*12 and positive or negative specific results of cockroach or mosquito allergens specific IgE based ELISA results in allergic patients revealed that the percentage of HLA - DQB*0602,HLA - DQB1*0604 and HLA - DRB1*12 alleles presence in cockroach allergic patients having positive results were higher than those ratios that have been observed in patients with negative ELISA results where HLA - DQB1*0602 appeared in high percentage)35.6%( followed by percentage( 15.3% )of HLA - DQB1*0604 . The relationship of HLADQB1and HLA - DRB1 alleles with the results of specific IgE based ELISA was statistically significant (χ2 : 10.412; DF : 3; p - value =0.01536989).Incontrast the percentage of HLA - DQB1*0602,HLADQB1*0604 and HLA - DRB1*12 alleles presence in mosquito allergic patients was found to be higher in negative ELISA results patients also the relationship of these HLA - DQB1 alleles with mosquito allergen specific IgE based ELISA results was not considared statistically significant (χ2 : 3.115 ;DF : 3 and p= 0.37423205).However HLA - DQB1 * 0602 allele was found in the highest percentage (20.6%), followed by allele HLA - DQB1 * 0604 (8.8%).Concerning the presence of HLA - DQB1*0602 ,HLA - DQB1*0604 andHLA - DRB1*12 in cockroach or mosquito allergic patients and healthy individuals,the HLA - DRB1*12 and HLA - DQB1*0602 allels were not found in healthy individuals.The genotyping results difference between cockroach allergic patients and healthy individuals was considered statistically extremely significant (χ2 : 74.485 ;DF : 5 and p =0) as well as the difference in the results of the genotyping of mosquitoes allergic patients and healthy people was statistically with very high significant (χ2 : 23.342 ; DF : 5 and p = 0.00029035).

دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية : دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية والوراثية عند العاملين في معمل البتروكيمياويات في محافظة البصرة والوراثية عند العاملين في معمل البتروكيمي == The Study of the effects of previous Ethylene Exposure on Some Physiological and Genetic Parameters in Workers of Petrochemical Plant In Basrah province

Author name: هبة ثاقب يسر
Supervisor name: سامي جبر المالكي | فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was conducted to investigate the genetic and physiological effects of some industrial chemicals on workers of the Petrochemicals Industrial Factory, in Basra Province , as it has been collecting 100 blood samples during the month of January and February of 2011 from each of the exposure in (ethylene section) and the control group of (employees of the University of Basra). and then the samples were divided on the basis of each of the years of occupational exposure to more and less than 15 years , age group to the larger and smaller than 50 years, smoking habits.The results showed that there was a significant decrease in the haemoglobin concentration HB, PCV, RDW and MCV in the more than15 years exposure group in comparison with the control group, while, no significance was detected in the RBC, MCH, MCV ,RDW,MCH and MCHC in less than15 years exposure group . Whereas, a significant decrease in the PCV for the same group was observed.White blood cells parameters’ results showed also variations in their values. In general, WBC did not change significantly in both groups of workers, while, the proportion of the lymphocyte and the of monocyte declined significantly in the more than 15 year of occupational exposure group, in contrast, the proportion of granulated cells increased markedly in the same group. The results of group of less than 15 years exposure revealed that the proportion of monocyte dropped significantly. Conversely, there was a significant increase in the proportion of granulated cells while the proportion of the lymphocyte was not significant . Platelets’ number and average of platelets’ volume decreased significantly in both occupational groupsTurning to the biochemical tests, the results showed significant increase in both liver enzymes ALT ,ASTand bili as well as this increase was found in urea, uric acid and glucose values in both occupational exposure groups. Contrariwise, the ALP creatinine and cholesterol did not reveal significant changes in both groups that in comparison with control group. The total protein decreased significantly in the group of more than 15 year of occupational exposure.Testosterone level revealed a significant decline in both occupational groups, while the Follicle stimulating hormone showed a significant increase in the occupational group of less than 15 year and no difference was observed in another occupational group while LH hormone level was not significant.In case of the genetic effects of the ethylene compounds, DNA was extracted for control and occupational groups and amplification was performed for GSTM1, GSTT1 and Albumin by using PCR technique. The results showed that there was no significant effect of both occupational periods on the GSTM1 gene, while GSTT1 gene was significantly in the workers samples in group more than 15 year of exposure, which was 2.43 times more than the group of less than 15 year of exposure.As regards the impact of exposure to ethylene and loss of genes GSTM1 and GSTT1 showed the results of our study, significant increase in the loss of gene GSTM1 amounted to more than five - fold (5.1) among workers exposed compared to a set of control while the proportion of loss of gene GSTT1 by more than three - fold (3. 1) compared to the group control has been a loss ratio of the two genes together more than eight - fold (8.5) for workers exposed group compared to the control.The light smoke did not reveal a significant effect on the null of GSTM1 and GSTT1 genes that in comparison with the control group, while the study found a significant effect of smoking on gene GSTM1, causing the null of much more than doubled (2.2) times compared with the control group (non - smokers) and the significant effect of smoking on null GSTT1 gene loss as increased visits by the text (1.5) compared with non - smokers also appeared that excessive smoking has an effect on the GSTT1 gene causing loss by seven - fold (7.08) once compared to non - smokers.The effect of the age group (less and more than 50 years old) of the workers on the null of both GSTM1 and GSTT1 was also examined. GSTM1 gene was null six times in both age groups of workers more than the control samples. While the GSTT1 was null in age group less than 50 years old three times more than the control group. As well as this absence was two times in the age group more than 50 years old in comparison with control group.It could be concluded from the present study that the exposure of workers to ethylene and its metabolic impact and clearly on blood standards as well as caused a high level of enzymes AST, ALT and bilirubin and high blood sugar levels and lower total protein and high level of urea and uric acid level as well as the low level of the hormone testosterone as it turns out that occupational exposure to ethylene and metabolic outcomes and length of exposure and smoking for workers exposed role in influencing the genes of detoxification, causing null in each of the gene GSTT1 and GSTM1.

دراسة مظهرية وجزيئية وبيئية للدور اليرقي بعد المذنبة لمثقوبة العين Diplostomum على ثلاثة انواع من اسماك نهر كرمة علي في محافظة البصرة == Morphological, Molecular and Ecological Studies of the larval stage (metacercaria) of eye trematode Diplostomum on three fish species from Garmat Ali River, Basrah Province

Author name: هاجر رزاق منهل
Supervisor name: عبد الحسين حبش
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:

الحساسية الغذائية للروبيان : دراسة وبائية ومناعية وجزيئية في محافظة البصرة جنوب العراق == Food allergy of shrimp : Epidemiological,Immunological and Molecular Study inBasra Province, Southern of Iraq

Author name: نور جاسم علي جودة
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 210 human venous blood samples were collected from Basrah province ( 190 randomly collected from people living in different regions of Basrah province , 10 blood samples from people who did not have a history of shrimp consumption or dealing with it used as negative control group, anther 10 samples of people who are sensitive to shrimp used as a positive control group). The samples were serologically tested by Enzyme Linked Immunosorbent Assay (ELISA) to estimate the distribution of total and specificIgE in the sera of studied individuals . Polymerase Chain Reaction (PCR) was performed to detect the HLA - DQB1*02 , *0302 , *0305 allels. The immunological study included the preparation of two types of Metapenaeus affinis allergens which are the raw allergens and the boiled allergens used in Indirect ELISA test to determin shrimp food allergies by serodiagnosis , while Direct ELISA test was used to estimate the distribution of total IgE by quantitative serological methods in the sera of studied samples. the concentration of protein in antigens extracts was estimated, the protein concentration in the boiled shrimp extract was less than its concentration in the raw extract.The results of direct ELISA test demonstrated a decrease in the level of total IgE antibodies in most of the studied samples , the level of IgE <100 recorded a significant high rate 98.4% which is more than the level of IgE > 100 which recorded apercentage of 1.6% and there was a significant difference between them. The Indirect ELISA test results revealed that the total percentage of IgE distribution of the raw and boiled shrimp antigens were 57.8% and 74.2% respectively and there was a significant difference between males and females .However , the difference was not significant between the age groups P>0.05.The molecular study was conducted to detect the presence of some alleles HLADQB1* 02, *0302, *0305 gene in the blood samples by using PCR . The current study showed that the association of the presence of these alleles and the level of total immunoglobulin is highly significant.Also the current study showed that a high significant difference was found between the positive samples to the studied alleles and the seropositive and seronegative results of raw and boild shrimp allergen specific IgE based on Indirect ELISA (P≤0.01).The results of PCR showed a high frequency ratio of HLADQB1 * 0302 97.2% in allergic patients of shrimp boild allergen and 56.9% in allergic patients of shrimp raw allergen .Also the current study showed a very high frequency ratio of HLADQB1 *0305 98.2% in the individuals who showed positive results for the raw allergen and recorded 65.2%. in the individuals who showed positive results for the boild allergen ,while HLADQB1*02 showed in one sample of studied samples which recorded positive results to raw and boild allergens of shrimp .The current study demonstrated also that the alleles frequency in some of studied samples which are seronegative by Indirect ELISA test , HLADQB1*0302 was 30.9% in raw antigens, while in the negative boild sample was not observed . HLADQB1*0305 was 1.23% in serongative of raw allergen and 16.32% in boild allergen. However, HLADQB1*02 was notobserved in seronagative to raw and boild shrimp allergen.

دراسة انتشار الديدان الحلقية وبعض الاحياء القاعية المتواجدة معها في منطقة القرنة / شمال محافظة البصرة

Author name: نور الهدى وليد عبد الرضا
Supervisor name: صبيح هليل المياح | مرتضى يوسف
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: درست اللافقريات القاعية المائية الكبيرة لنهر الفرات وشط العرب واختيرت ثلاث محطات للدراسة تقع المحطة الاولى تقع على نهر الفرات بينما تقع المحطة الثانية بعد نقطة التقاء نهري دجلة والفرات بحوالي 2 كيلو متر في حين تقع المحطة الثالثة بعد التقاء شط العرب ونهر الغميج. جمعت العينات شهريا ابتداء من تموز2013 - ولغاية حزيران2014 - بواقع ثلاث مكررات من كل محطة. تم خلال الدراسة الحالية قياس بعض العوامل للبيئة المائية (درجة حرارة الماء والملوحة والاس الهيدروجيني والاوكسجين المذاب) اذ تراوحت درجة الحرارة بين 32 ̊م و18.4 ̊م وتراوحت الملوحة بين 3.8 - 1.9 جزء بالالف والاس الهيدروجيني بين 8.13 - 6.96 والاوكسجين المذاب بين 16 - 7.6 ملغرام/لتر. درست ايضا بعض العوامل الخاصة بالقاع (نسجة القاع والاس الهيدروجني والملوحة والكالسيوم والفسفور والنتروجين والبوتاسيوم والمادة العضوية) وجد ان نسجة القاع كانت غرينية طينية مزيجية في المحطة الاولى بينما كانت غرينية في المحطة الثانية وغرينية مزيجية في المحطة الثالثة, وتباينت عوامل القاع الاخرى بين محطات الدراسة الثلاثة اذ تراوحت معدلات قيم الاس الهيدروجيني بين 7.55 و7.24 والملوحة بين 5.3 3.2 جزء بالالف والنتروجين 150 - 42 جزء بالمليون (ppm) والفسفور 53.3 - 29.8 جزء بالمليون والبوتاسيوم بين 346 - 226 جزء بالمليون والكالسيوم 986.6 - 480 جزء بالمليون والمادة العضوية 48 - 10 % . سجلت خلال الدراسة ثلاث مجاميع من اللافقريات القاعية ظهرت في محطات الدراسة جميعها وهي النواعم بطنية القدم Gastropoda والديدان الحلقيةAnnelida والحشراتInsecta , اذ سجلت 25 و16 و20 من المراتب التصنيفية في المحطات الثلاثة على التوالي, وسجلت 4 انواع من النواعم ومرتبة واحدة من الحشرات في كل من المحطات الثلاثة اما الديدان الحلقية فسجل منها 20 و11 و15 نوعا في المحطات الثلاثة على التوالي. وسجلت خلال الدراسة الانواع Potamothrix hammoniensis ( Michaelsen,1901) وPotamothrix bavaricus (Öschmann,1913) وPsammoryctides moravicus (Hrabe, 1934) من مجموعة ديدان النايدد الانبوبية وNais stolci (Hrabe,1981) وParainais frici Hrabe, 1941 من مجموعة النايدد لاول مره في جنوب العراق عموما بينما يسجل النوعين Nais raviensis Stephenson,1941 وHomochaeta lactea (Cernosvitov,1937) من مجموعة النايدد لاول مره في العراق. جمعت خلال فترة الدراسة 4497 عينة من اللافقريات القاعية (1692 و1169 و1636من محطات الدراسة الثلاثة على التوالي) مثلت الديدان قليلة الاهلاب نسبا كلية عالية في المحطات جميعها 90.6) % و85.2 % و87% على التوالي) اما ادنى النسب فكانت لعديدة الاهلاب وبلغت 0.15 % و0.2 % سجلت في المحطتين الاولى والثالثة على التوالي. ضمن مجموعة بطنية القدم اخذ النوع Melanoides tuberculata اعلى النسب (55.2 % و43.7 % و30.4 % سجلت في محطات الدراسة الثلاثة على التوالي) اما النسبة الاقل فبلغت 9.7 % وسجلت للقوقع Physa acuta في المحطة الثانية. فيما يتعلق بمجموعة الديدان الانبوبية وجد النوع Limnodrilus hoffmeisteri بنسب كلية بلغت 96.9 % في المحطة الاولى و98.6 % في كل من المحطتين الثانية والثالثة اما النسبة الاقل فبلغت 0.1 % وسجلت للنوع Tubifex tubifex في المحطة الاولى و0.2 % و0.5 % وسجلت للنوع P. moravicus في المحطتين الثانية والثالثة على التوالي. اما اعلى النسب بالنسبة للانواع العائده لمجموعة النايدد فبلغت 34 % و78.3 % وسجلت للنوع N. stolci في المحطة الاولى والثالثة على التوالي واما في المحطة الثانية فوجدت الدودة Pristinella sima بنسبة 30 % , اما النسبة الاقل من بين جميع المحطات فسجلت لكل من النوعين Pristina aequiseta وDero nivea وبلغت 0.8 % . درست الكثافة الشهرية للانواع المسجلة خلال الدراسة وبلغت اعلى القيم 5082 و2058 و5430 فرد/م2 في محطات الدراسة الثلاثة على التوالي وجميعها سجلت للنوع L. hoffmeisteri , اما ادنى القيم فبلغت 11فرد/م2 وسجلت في جميع المحطات لعدد من الانواع. درست ايضا النسبة المئوية لتكرار لظهور الانواع ووجد ان النوع L. hoffmesteria هو النوع الوحيد الذي ظهر خلال جميع الاشهر وفي جميع محطات الدراسة, كما ظهر باعلى نسبة تكرار (%100) في جميع اشهر الدراسة في المحطتين الثانية والثالثة ولثمانية اشهر في المحطة الاولى اما نسبة التكرار الاقل فبلغت 33 % وسجلت لجميع المحطات ولعدد من الانواع. قورنت المحطات احصائيا من حيث عوامل الحرارة والملوحة وpH والاوكسجين المذاب وكذلك كثافات النواعم وقليلة الاهلاب وعدد الانواع, ولم تسجل فروق معنوية سوى الاوكسجين المذاب الذي اختلف معنويا بين المحطتين الاولى والثانية من جهة والثالثة من جهة اخرى. اما فيما يتعلق بتحليل الارتباط فقد وجد ان معدل الحرارة ارتبط ايجابا مع كل من معدل الاس الهيدروجيني (r = 0.679) ومعدل الملوحة (r = 0.350 ). من جهة اخرى ارتبط معدل الملوحة ايجابا مع معدل كثافة النواعم (r = 0.331) وارتبطت الحرارة سلبا مع كل من عدد الانواع الكلية المسجلة (r = - 0.361) وكثافة قليلة الاهلاب الكلية. وارتبط عدد الانواع الكلية بشكل موجب مع كثافة قليلة الاهلاب الكلية (0.395 r=). | Aquatic macro - benthic invertebrate of the Euphrates and the Shatt al - Arab Rivers were investigated for the period from July 2013 until June 2014. Three stations were selected to execute the study the former is located in the Euphrates River, while the second is situated about two kilometers apart from the confluence of the Tigris and Euphrates. The third station, however, is located after the confluence of the Shatt al - Arab River with Ghamaj canal. Three replicate samples were collected from each station. some environmental factors were measured ( i.e. water temperature , salinity, pH and dissolved oxygen ) water temperature ranged between 18.4ºC to 32º C during January and May respectively, salinity ranged from (1.9 - 3.8) ppt. during October and May respectively, pH between 6.96 - 8.13 during December, March and April respectively, dissolved oxygen between (7.6 - 16) mg/L during July and September respectively Some other factors concerning the bottom and substrate were also studied namely, bottom texture, pH, salinity, calcium, phosphorus, nitrogen, potassium and organic matter. The study found that the bottom texture was silty clay in station1 while silty in the second and silty mixture in the third station. Other bottom components varied between the three stations. However, pH ranged between 7.24 and 7.55, salinity between (3.2 - 5.3) ppt. Nitrogen from 42 - 150 ppm, PO4 (29.8 - 53.3) ppm, potassium (226 - 346) ppm and Ca+2 from 480 - 986.6 ppm., and organic matter from (10 - 21)%. Three categories of benthic invertebrates were encountered in the studied stations i.e. Gastropoda, Annelida and Insecta, as 25, 16 and 20 taxonomical orders of each respectively. four molluscan species and one order of insects in each of the three stations, while annelida a total of 20 , 11 and 15 species were found in the three stations respectively . the following species were collected during the study Potamothrix hammoniensis (Michaelsen, 1901), Potamothrix bavaricus (Öschmann, 1913) and Psammoryctides moravicus (Hrabe, 1934) of a group of worms Naadd tube and Nais stolci (Hrabe, 1981) and Parainais frici Hrabe, 1941 from Naadd group for the first once in southern Iraq, while generally registers types Nais raviensis Stephenson, 1941 and Homochaeta lactea (Cernosvitov, 1937) of Naadd group for the first time in Iraq. a total of 4497 specimens of benthic invertebrates were collected during the study period (i.e. 1692, 1169 and 1636 from three stations respectively) oligochaetes formed a high proportions in all stations (90.6, 85.2 and 87 %, respectively) and the lowest percentage was for polychaetes amounted to 0.15% and 0.2 % recorded in the first and third stations respectively . out of gastropods Melanoides tuberculata formed the highest proportions ( 55.2 % and 43.7 % and 30.4 % recorded in the three study stations respectively ), but the lowest ratio was 9.7% recorded for the snail Physa acuta in station 2. With respect to tube worm’s group Limnodrilus hoffmeisteri was dominant proportions accounted for 96.9 % in station 1 and 98.6 % in other stations. However, the lowest proportion (0.1 %) was recorded for Tubifex tubifex in station1 and 0.2 % and 0.5% recorded for the species P. moravicus in the stations 2 and 3 respectively. The highest species proportions belong to the group Naididae amounted to 34% and 78.3 % recorded for the species N. stolci in stations 1 and 3 respectively, while in the station2 the worm Pristinella sima was found by 30%. The lowest proportion among all stations was recorded for Pristina aequiseta and Dero nivea amounted to 0.8%. Monthly density of the species recorded during the study was calculated and reached the highest values of 5082, 2058 and 5430 individual / m 2 in the three investigated stations respectively , all recorded for the species L. hoffmeisteri, the lowest value, however, was 11 individual / m 2 were recorded in all the stations for a number of species. Percentage frequency for the species was also studied and found that the species L. hoffmesteria is the only one that occurred during all months in all study stations, as shown by the highest percentage of recurrence (100%) in all months in the stations 2 and 3 , and just eight months in stations1. the lowest frequency (33%) recorded for all stations for a number of species. Stations statistically compared concerning temperature, salinity, pH and dissolved oxygen, as well as densities of molluscs, oligochaetes and the number of species. Significant differences were not found except with dissolved oxygen, which differed significantly between the first and second stations on the one hand and the third on the other hand. Regarding correlation it has been found that mean temperature was positively associated with both pH (r = 0.679) and the mean of salinity (r = 0.350). On the other hand, mean salinity was positively associated with molluscs density (r = 0.331) and temperature was negatively associated(r = - 0.361) with both the number of total species recorded and density of oligochaetes . The total number of species was positively associated with oligochaetes density (r=0.395).

دراسات تصنيفية وحياتية ووبائية للمثقوبات المتطفلة في الجهاز التنفسي للطيور المائية في اهوار محافظة ذي قار == Taxonomic,Biological and Epidemiological Studies of Trematodes Parasitic in the Respiratory System of Some Aquatic Birds in Marshes of Thi - Qar province/Iraq

Author name: نثيلة رشيد حميد الكسار
Supervisor name: صبيح هليل المياح | باسم هاشم عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 420 aquatic birds belonging to four species( Chettusia leucura , Himantopus himantopus, Gallinula chloropus and Fulica atrawere collected in the current study during the period between December 2012 to November 2013 fisolated from Al - Chibayish marshes , eastern of Thi Qar province. Digenetic trematode, Haematotrephus nittanyense was isolated from H. himantopus and C. leucura. Prevalence in those birds were 20.7% , 2.2% respectively. Mean intensity of infection 1.7 , 1.2 respectively. Digenetic trematode , Haematotrephus chengi was found in C.leucura, the Prevalence of infection was 20.7% . These two trematodes were recorded for the first time in Iraqi birds . 2.6% of Ch. leucura were infected with Digenetic trematode , Uvitellina iraquensi , mean intensity of infection was 2.7 . Digenetic trematode , Cyclocoelum mutabile Cyclocoelum mutabile was isolated from F. atra and G. chloropus birds . Prevalence in those birds were 21.2% ,10% respectively. Mean intensity of infection 3 , 3 . Digenetic trematode , Cyclocoelum microstomum was found in 20% of G. chloropus birds and in 15% of F. atra , mean intensity of infection 2.8 , 1.8 respectively.The current study revealed that , most eggs of H. nittanyense , H. chengi and U. iraquensis hatch within the uteri of worms while embryonated eggs of C. mutabile and C. microstomum shed from mature worms hatch within few hours after being placed in water. The mean number of eggs differed between species. Hatching of C. mutabile and C. microstomum eggs did not occur at 12°C . Egg hatching rate of C. mutabile differed from eggs hatching rate of C. microstomum at all temperatures . Temperatures, salinity and acidification of water affectedthe rate of hatching of C. mutabile and C. microstomum eggs , and the results varied between two different species . Storing eggs of C. mutabile and C. microstomum at (0,4)°C for ( 1 - 8) weeks failed to hatch . Storing eggs of C. mutabile and C. microstomum at (7,10,12)°C for (1 - 8) weeks had no effect on hatching success or on eggs hatching rate.A biological study of the miracidia of trematodes which were isolated in the current study showed that , the survivorship and longevity of miracidia were differed among the different species . Temperature, salinity and acidification of water affected the survivorship and longevity of miracidia . Storing eggs of all species at (7,10,12)°C for eight weeks had no effect on survivorship and longevity of their miracidia. The study revealed that , the miracidia of all species were infective to Radix auricularia and Physa acuta snails , but R. auricularia snails more sensitive to infection . The infectivity of the miracidia of all species to R. auricularia and P. acuta snails varied according to the species of these trematodes . Infection rate of R. auricularia snails with the miracidia of all species , mean intensity of infection and the metacercariae output varied according to species of these trematodes, temperature , miracidial density as well as size of snails. Miracidia hatching from eggs stored for 1 - 8 weeks at 7°C not differed in their infectivity to snail. The miracidia of all species hatching from eggs stored for (0 - 8) weeks at 7°C differed in their infectivity to snail according to the species of these trematodes. Age and density of H. chengi miracidia affected the infection rate of R. auricularia snails as well as affected the mean intensity of infection . H. chengi infection had no effect on mortality rate of R. auricularia and P. acuta snails.The results showed that there were differences between the various stages included in the life cycle of the five species of Cyclocoelidae family isolated in the current study . The time required for production and development of these stages varied according to their species, as well as these larval stages were differed among the species in their shapes , sizes and in the product number of them .The current study revealed that , metacercariae of the trematodes which were isolated in the current study fed separately to Gallus domesticus and Anas platyrhynchos failed to produce an infection , although the influence of C. leucura bile on metacercariae of H. nittanyense , H. chengi and U. iraquensis varied according to the species of these trematodes but bile of C. leucura plays a role in increasing excysted juveniles ratios , reduction the time required for excysting and increasing the time of juveniles activity compared with a bile of G. domesticus .In the current study. H. nittanyense showed a clear seasonal cycle of infection in local aquatic birds , H. himantopus ,while the study exposed a clear seasonal cycle of H. chengi infection in local aquatic birds C. leucura . Isolation of U. iraquensis in different periods during the current study from local aquatic birds, C. leucura may refer to its ability to complete its life cycle in a study area. Isolation of C. mutabile from migratory bird at arrival time to the study area may refer to new infections that carried by these migratory birds from their breeding grounds . Abundance of sensitive snails and suitable temperatures in a study area at arrival time of these birds may help this trematode to complete its life cycle in this area thus new infections emerged later and these infections may carry by microstomum isolated from migratory birds only at the arrival time to the study area may refer to new infections that carried by these migratory birds from their breeding grounds as well as this may indicate an inability to complete the life cycle of this trematode in this area.

حياتية القوقع الدخيل Pomacea canaliculata Lamarck, 1822 في شط العرب - محافظة البصرة == Biological of the Invasive Snail Pomacea canaliculata (Lamarck, 1822

Author name: نادية جعفر كاظم البغدادي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was concerned with some biological aspects of the invasive snail Pomacea canaliculata (Lamarck, 1822) and for this purpose, monthly samples from Shatt Al - Arab banks in the area of Al - Salhia were collected during the period from October 2014 to September 2015. The study included the monthly variations in the density of snail , its egg masses and the population sizes. In the laboratory, the study included many aspects, these are the relationships between female weight with some measurements of its egg masses, embryonic development duration, hatching percentages under different temperatures, food consumption, salt acclimatization, growth under combined effect of salinity and temperature, effect of food type on growth of newly hatched snails and examine samples of snail to detect the infection with parasites if present. The density of the snail was ranged from four to 13 ind. /m2 recorded during January - February 2015 and October 2014 respectively, while the mean density of egg masses was from two to nine egg mass / m2 recorded during April 2015 and October 2014. The statistical analysis showed that the temperature had positive correlated with density of each of snails and its egg masses. On the otherhand, there are positive correlation between the density of each snail and its egg masses. The snails, which had been collected during the study period, were 19 - 68 mm in length, these measurements were classifying into five size classes. The size class 35 - 45 mm was dominanted for nine months, compared with the size class 55 - 65 mm that had less occurrence.The diameter of eggs varied from 2.5 - 4 mm. The 25°C is the most suitable temperature to get a high percentage of hatching and a short period of embryonic development. There was no significant correlation between the weight of the female and the number of its egg masses.The feeding experiment showed that the amount of food consumed lettuce mild during the study period 30 days by one individual ranged from 3 - 29g. The results of the acclimatization was referred to the ability of all size classes' snails to tolerate the low salinity 2.5 psu with 100% survival percentage, while the snails of size classes at least 10 mm and 10 - 25mm were able to tolerate the salinity of 11psu with 10% and 50% respectivly survival.There are variable effects of the combination of temperature and salinity on growth of newly hatched snails for 9 weeks experiment, these effects were reflected on the weight, length and width, and the highest of these measurements were recorded for snails of 2.5 psu - 27°C combination, while the lowest one was recorded in 7.5 psu - 10°C. The lowest survival percentage was 5% recorded for the individuals of 7.5 psu - 27 and 32°C, compared with 90% of distil water - 17°C. On the other hand, the optimum growth was in 2.5 psu all temperatures, while the optimum growth was in all salinities, and temperature of 27°C.The experimental study of five types of food fresh celery, fresh lettuce, fish food, mixed food and animal protein)on growth of newly hatched snails for 9 weeks. Showed that the mean weight, length and width of snails were depending on the food type. The mixed food gave significant increase in growth 382.1 mg weight, 10.10mm length and7.58mm width compared with the other types of food.The highest survival percentage 95% was recorded for the snails fed on celery, lettuce and fish food. The present study on the snail P. canaliculata does not record any infection by the larvae of parasites

دراسة المسببات الميكروبية الشائعة للاسهال لدى الاطفال دون خمس سنوات من العمر وتوصيف عاثية الامعاء

Author name: مروة محمود يعقوب
Supervisor name: كوثر هواز مهدي | حيدر عبد الحسين مكلف
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The purpose of present study was to detection of Rotavirus A and Escherichia Coli from diarrhea cases in children, and characterization of E.coli phag, in addition to study some of factors that influence on Diarrhea.Stool samples were collected between 15/11/2014 and 1/4/2015 from children 0 to 59 months of age who were hospitalized in Basra hospital for women and children, Basra/Iraq. A total of 300 children with acute gastroenteritis were enrolled, including 199 males and 101 females.In this study among children with Diarrhea was 93/300 (31%) Rotavirus positive cases by Immunochromatographic (IC) test as monoinfection (1.66%), coinfection (26.34%), and mixing infections (3%). Out of 50 IC positives fecal samples were tested using electronic microscope, 50(100%) were found positive. A total of 80 stools were examined for Rotavirus using polyacrylamide gel electrophoresis. The overall agreement was 68/80(85%).Furthermore, regardless of the types of infection, monoinfection or coinfection or mixing, Bacteria was isolated, including; 277/300 (92.33%) children had infections with Escherichia coli, 39/300 (13%) cases with Salmonella spp., While, Shigella spp. was reported in 12/300 (4.01%) samples. The identification of bacterial pathogens was later confirmed by Api 20 Enterobacteraceae system. Also parasitic causes were found in 6/300 (2%) samples.Overall, this study recorded 182 /300 (60.66%) of monoinfection cases including; 5 /300 (1.66%) with Rotavirus, 163 /300 (54.33%) with Escherichia coli, 6 /300 (2%) with Salmonella spp., 2 /300 (0.67%) with Shigella spp., 3 /300 (1%) with Entamoeba histolytica, 3 /300 (1%) with Giardia lamblia. Coinfection with another microorganism was observed in 109/300 (36.34%) cases, coinfection with Rotavirus and Escherichia coli were the most common and occurred in 75/300 (25%) cases.Antibiotic susceptibility testing was performed on Muller Hinton agar against five different antibiotics to 30 isolates of E. coli. The antibiotic resistance/susceptibility profile of E. coli isolates revealed that most of the isolates were resistant to three tested antibiotics (Trimethoprim, Ampicillin and Doxycyclin). The phage ɸEC - MH1 was isolation successfully from sewage. The phage titer was determined by serial dilution (10 - 1 to 10 - 9) of the sample by counting the number of plaque forming units (p.f.u.) for each dilution.Results revealed that dilution factor 10 - 2 was the best countable number of plaques. The statistical analysis was significantly decrease P≤ 0.05 in phage titer at the temperature 50C° and 65C° during different times comparing with phage titer at the temperature 37C°. Effects of chloroform on phage titer during different times were completely inactivated comparing with saline environments. Furthermore, regarding some clinical information; the present study showed that 66.33% of the males were more susceptible to the infection with highly significant (P<0.001) than females (33.67%). Mean age (mean ± standard deviation) of all positive cases was 11.02±12.3 months, and the age periods between (0 - 5) and (6 - 11) were significantly prone to the infection.Also, all cases were complained with diarrhea (100%), fever (82.34%), Vomiting (43%) then dehydration (2.67%). In addition, all positive cases become increased in December (32%) and city center (81%). This study showed positive cases increase (69%) of children with artificial feeding

قابلية بعض الطحالب الخضر - المزرقة المعزولة من بعض المسطحات المائية في محافظة البصرة / جنوب العراق على انتاج السموم == Capability of some blue - green algae isolated from some water bodies in AL - Basrah Governorate / Southern Iraq to the production of toxins

Author name: مروة عبد الكريم عبيد
General topic: Biology
Specific topic: plant - aquatic life
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: تضمنت الدراسة الحالية تشخيص وعزل وتنقية واكثار ثمانية انواع تابعة لثمانية اجناس من الطحالب الخضر - المزرقة من بعض المسطحات المائية لمحافظة البصرة جنوب العراق والتي تمثلت بكل من شط العرب ونهر العشار ونهر الخندق وهي كل من الانواع Merismopedia glauca وMicrocystis flos - aqueوNostoc commune وOscillatoria pseudogeminata واربعة منها عدت كتسجيل اول في العراق وتمثلت بكل من الانواع Lyngbya rubida وPseudanabaena limnetica وPhormidium laysanense وStigonema informe وقد شخصت قابليتها على انتاج كل من السم العصبي Anatoxin - a والسموم الكبدية Microcystins كما ونوعا لاول مرة محليا وعالميا. تم قياس منحنى النمو للانواع المعزولة والمنقاة بعد تنميتها في الوسط الزرعي السائل Chu - 10 كما قيس كل من ثابت النمو(k)Growth constant والذي بلغ 0.195 و0.197 و0.246 و0.25 و0.278 و0.29 و0.528 وزمن تكاثر الجيل (G)Generation time بلغ 1.543 و1.527 و1.223 و1.204 و1.082 و1.037 و0.570 يوم لانواع الطحالب M. glauca وM. flos - aque وP. limnetica وPh. laysanense وO. pseudogeminata وL. rubida وN. commune على التوالي. نقي السم العصبي Anatoxin - a والسموم الكبدية Microcystins من انواع الطحالب الخضر - المزرقة المعزولة والمنقاة خلال الدراسة الحالية وشخص كما ونوعا بواسطة تقنية الامتزاز المناعـــــي المرتبــــــــط بالانزيــــــم Enzyme Linked Immuno Sorbent Assay (ELISA) وقـــد حددت تراكيز السم العصبي المنقــى من الانواع M. flos - aque وN. commune وL. rubida وS. informe وPh. Laysanense وO. pseudogeminata وM. glauca وP. limnetica والتي بلغت 0.357 و0.511 و0.598 و0.635 و0.705 و1.119 و1.156 و1.179مايكروغرام/ لتــــر علــــى الترتيب , اما فيما يخص قابليــة الانـــواع على انتاج السموم الكبدية فقد بلغت 0.973 و6.128 و1.309 و1.753 و6.209 و3.855 و1.733 و3.701 مايكروغرام/لتر على الترتيب . اوضح اختبار طيف الاشعة فوق البنفسجية امتلاك السم العصبي Anatoxin - a المنقى قمة امتصاص واحدة عند الطول الموجي 226 نانومتر, وكذلك اظهرت السموم الكبدية المنقاة قمة امتصاص واحدة عند الطول الموجي 240 نانومتر , كما بين اختبار طيف الاشعة تحت الحمراء احتواء كلا النوعين من السموم على معظم المجاميع الفعالة المكونة للسموم القياسية لها. حددت الجرعة نصف القاتلة (LD50) Lethal dose concentration للكتل الحية للانواع الثمانية تجاه يرقات الحيوان اللافقري القشري Artemia salina وقد اظهرت النتائج ان للطحالب المعزولة والمنقاة سميـــة عالية بدرجات متفاوتة تجاه يرقات الحيوان القشري وان الانواع المنتجة للسم العصبي Anatoxin - a وبتراكيز عالية كانت الاكثر سمية لليرقات مقارنة بالانواع الاخرى المنقاة خلال الدراسة ، اذ بلغت نصف الجرع القاتلة لها 29.183 و30.66 و30.716 و33.466 و34.566 و35.866 و36.933 و37.8 مليغرام/مليلتر لكل من الطحالب P.limnetica وM. glauca وO. pseudogeminata وS.informe وPh. Laysanense وN. commune وL.rubida وM.flos - aque على الترتيب . كما حددت الجرعة نصف القاتلة (LD50) للسم العصبـــيAnatoxin - a المنقى من الطحلب الاخضر المزرق P.limnetica تجاه يرقات الحيوان اللافقري القشري A. salina والتي بلغت 0.498 مايكروغرام/لتر ، وحددت ايضا الجرعة نصف القاتلة للسموم الكبدية Microcystins المنقاة من الطحلب الاخضر المزرق Ph. Laysanense والتي بلغت 2.59 مايكروغرام/لتر . درست التاثيرات النسجية للسم العصبي Anatoxin - a المنقى من الطحلب الاخضر المزرق السام P. limnetica لاول مرة محليا وعالميا على دماغ الفئران المختبرية البيضاء نوع Mus musculus L تحت تراكيز واطئة من السم العصبي هي 0.5 و1 مايكروغرام/لتر لفترة تعرض امتدت لخمسة عشر يوما وقد لوحظ ازدياد التاثيرات بازدياد التركيز, وقد اظهرت نتائج الفحص المجهري عند التركيز 0.5 مايكروغرام/لتر بداية حدوث كل من حالة التفجي Vacuolation والتنخر Necrosis في منطقة المادة البيضاء White matter بالاضافة الى تغلظ انوية الخلايا المساعدة Karyopyknosis وبداية احتقان الاوعية الدموية Congestion واضمحلال النسيج الطلائي المبطن لها , كما اظهر الفحص حدوث انكماش Shrinkage للخلايا العصبية المكونة لمنطقة المادة الرمادية Gray matter فضلا عن حدوث انحسارا في سمكها مقارنة مع مجموعة السيطرة وقد ازدادت التاثيرات بازدياد التركيز الى 1مايكروغرام/لتر فقد لوحظ اضافة الى التاثيرات السابقة اضمحلال كبير في سمك المادة الرمادية وقلة نسبة الخلايا العصبية فيها مع ازدياد حالات الانكماش ، اضافة الى اضمحلال الخلايا المساعدة واختفاء الخلايا الاندوثيلية المبطنة للاوعية الدموية وانتفاخ بعضها وحدوث التوسع Dilation والاحتقان بشكل اكبر , كما لوحـــظ انه وبارتفاع التركــــيز ازدياد حالة التفجـــي وظهور المادة البيضاء بشكل اسفنجي Spongiform shape وازداد التنخر وظهور حالات فرط التصبغ Hyperpigmentation لسايتوبلازم الخلايا بازدياد التركيز مقارنة مع مجموعة السيطرة. كما سجلت بعض التاثيرات السلوكية للسم العصبي Anatoxin - a على الفئران المختبرية اثناء فترات الحقن بالسم العصبي تمثلت بفقدان الوزن اذ وجد انخفاضا معنويا p≤0.05 في معدل اوزان الفئران المعاملة بالسم بعد خمسة عشر يوما لكلا التركيزين مقارنة مع فئران مجموعة السيطرة بالاضافة لحدوث فقدان البصر(العمى) وتساقط الشعر بشكل واضح بعد (7 - (10ايام من الحقن . | The current study included the identification, isolation, purification and cultivation of eight species belonging to eight genus of blue - green algae from some of the water bodies of Basrah Governorate/ Southern Iraq,These included Shatt al - Arab river, Al - Ashar and Al - kandak River. The algal species included Merismopedia glauca, Microcystis flos - aque, Nostoc commune , Oscillatoria pseudogeminata, Four of them were first recorded in Iraq : Lyngbya rubida, Pseudanabaena limnetica, Phormidium laysanense and Stigonema informe. Their ability to produce both anatoxin - a and microcystins was quantified and qualified determined for the first time locally and globally.The growth curve of isolated and purified species was measured after their development in the Chu - 10 liquid medium as measured the growth constant(k) which reached to 0.195, 0.197, 0.246, 0.25, 0.278, 0.29 and 0.528, and the generation time(G) which reach to 1.543, 1.527 and 1.223, 1.204, 1.082, 1.037 and 0.570 hour for the algal species M. glauca, M. flos - aque , P. limnetica, Ph. laysanense, O. pseudogeminata, L. rubida and N. commune , respectively. Neurotoxin (Anatoxin - a) and hepatotoxin (Microcystin) was purified from the blue - green algal species isolated and purified during the current study and quantified and qualitatively by Enzyme Linked Immunosorbent Assay (ELISA). The concentration of purified neurotoxin has been identified as algae M. flos - aque, N. commune , L. rubida, S. informe, L. laysanense, O. pseudogeminata, M. glauca and P.limnetica, which were 0.357, 0.511, 0.598, 0.635, 0.705, 1.119, 1.156 and 1.179 μg/L respectively, While concerning the ability of the species to produce hepatotoxin (Microcystins) it has reached 0.973, 6.128, 1.309, 1.753, 6.209, 3.855, 1.733 and 3.701 µ/L respectively.The Ultraviolet spectrum test showed that the neurotoxin ( Anatoxin - a) had a single absorbance peak at a wavelength of 226 nm , and also the purified hepatotoxin (MCs) showed single absorbance peak at wavelength 240 nm. The infrared spectrum test showed that both types of toxins were present on most of the active constituents or active groups of their standard toxins.The half lethal dose concentration (LD50) of the biomass of the eight species of blue - green algae was determined towards the animal crustacean invertebrates larvae Artemia salina. The results showed that the algal species had high toxicity towards the crustacean animal larvae and that the algae that produce the anatoxin - a at higher concentrations compared to other algae in this study was the most toxic to the larvae as the LD50 reached 29.183, 30.66, 30.716, 33.466, 34.566, 35.866, 36.933 and 37.8 mg / mL for the algae P. limnetica, O. pseudogeminata, M. glauca and S .informe, L. Laysanense, N. commune , L.rubida and M. flos - aque respectively. The half lethal dose concentration (LD50) of neurotoxin(Anatoxin - a) purified from blue - green alga P. malimnica against the larvae of Artemia salina, which reached 0.498 μg/L, while the half - lethal dose concentration reach to 2.59 μg/L for purified hepatotoxin (Microcystin) from the blue - green alga Ph. Laysanense The histopathological effects of neurotoxin anatoxin - a purified from P. limnetica for the first time locally and globally on the brain of white mice type Mus muscules L. were studied under low concentrations of neurotoxin (Anatoxin - a) 0.5 and 1 μg / L for a 15 - day exposure period and increased effects were observed with increased concentration. The results of the microscopic examination at concentration 5µg/L showed begin of occurrence vacuolation ,necrosis in white matter in addition of karyopiknosis of glial cells nuclei, and the beginning of congestion of blood vessel and the decay of the epithelial tissue lining the blood vessel. As demonstrated by the examination the begin of occurrence shrinkage of neuron constituent of the gray matter area as well as less thickness of the gray matter compared with control group . The effects were increased with an increase in concentration to 1 μg/L. In addition to the previous effects, there was a significant deterioration in the thickness of the gray matter and the low percentage of neurons with increasing shrinkage in addition to decay of glial cells in white matter and disappearance or swelling of endothelial cells lining the blood vessel and the expansion dilation , greater congestion , vacuolation and appearance of white matter in spongiform shape, addition to increased necrosis, and hyperpigmentation compared to the control group.Some of the behavioral effects of anatoxin - a were recorded on laboratory mice during periods of injection of neurotoxin represented by weight loss. A significant decrease under probability level p ≤ 0.05 was observed in the weight of mice treated with both concentrations of toxin after 15 days compared with mice in control group , In addition to the loss of sight (blindness) and hair loss thick after 7 - 10 days of injection

السيطرة الاحيائية واللااحيائية على نمو الفطر Aspergillus flavus والتعبير الجيني للجينين aflDو aflR وانتاج الافلاتوكسين B1 == Biotic and Abiotic Control on Aspergillus flavus Growth, aflD and aflR Expression and Aflatoxin B1 Production

Author name: لبيد عبد الله نجم السعد
Supervisor name: عدنان عيسى البدران | سامي عبد الرضا الجميلي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Basrah
First pages:
Abstract: Fifteen isolates of Aspergillus flavus (AFL1 - AFL15) were isolated from multiple sources included soil, compost, seeds, fruits, feed and air. The isolates were identified morphologically then confirmed by molecular techniques. Only thirteen isolates were confirmed positively (AFL1 - AFL5 and AFL8 - AFL15) while two of them were not. A phylogenetic analysis was made which revealed that the isolates grouped according to their source of isolation. The aflR profile and phenotypic AFB1 production confirmed that all thirteen isolates posses the ability to produce AFB1 with significant differences (P ≤ 0.05) among them. Growth rate profile was performed under 30ºC which showed significant differences (P ≤ 0.05) among isolates. According to the above tests, A.flavus AFL14 was selected to be the experimental isolate for the rest of experiments in this study. Comparing to A.flavus NRRL3357 type strain, the impact of temperature, water activity aw and isolate factors was examined to determine the limits and optimum ecological conditions of growth of A.flavus AFL14. The results displayed that the best growth rate was (7.217 mm/day) at 0.98 aw where the lowest was (4.069 mm/day) at 0.9 aw with no growth at 0.85 aw while the impact of temperature demonstrated by outweigh of growth rate at 35ºC (6.201 mm/day) followed by 30ºC (5.272 mm/day) which exceeded 25ºC and 40ºC (4.604 and 4.051 mm/day), respectively.Bacillus subtilis (isolates : BSS1, BSS2, BSS3, BSS4 and BSW) and Pseudomonas fluorescens (isolates : PFMst and PFDL) were elected to be used as biocontrol agents. All the isolates were passed the biochemical identification tests moreover, BSS4 and BSW isolates of B.subtilis and PFMst and PFDL isolates of P.fluorescens were confirmed molecularly which used later in all biocontrol experiments in this study. The ability ofbiocontrol agents to inhibit fungal growth was investigated and the resultsshowed significant inhibition impact represented by significant outweighof B. subtilis BSS4 (99%) on the rest of B. subtilis isolates followed by P.fluorescense PFDL and PFMst (92.29 and 86.19) %, respectively, with nosignificant differences (P ≤ 0.05) among them. The extra - cellular abilityof biocontrol agents to degrade AFB1 showed a high degradation abilityIpreformed by B. subtilis BSS4, BSW isolates and P. fluorescence PFMst,PFDL isolates (100, 100, 97.805 and 97.396%), respectively. Thedegradation residues administrated to rats to determine their effect onbiosystems, the blood parameters showed a significant reduction ofWBC, HB, RBC, and P.C.V while there was a significant increase inUrea, Glutamic pyruvate transaminase (GPT) Glutamic oxaloacetictransaminase (GOT) and MCV in AF+DMSO treatment comparing tothe negative and positive control. Water activity and biocontrol factorswere examined to control aflD and aflR expression and phenotypic AFB1production. When the experiment held using Nutrient Agar medium, asignificant inhibition of aflD and aflR expression (P=0.000 and 0.003),respectively performed by BSW at 0.94 aw comparing to 0.98 aw whileno significant inhibition was observed in the rest of the treatments. Allbiocontrol agents revealed high significant reduction of aflD and aflRexpression (P≤0.001) in each water activity level separately whichconfirmed by HPLC results. The phenotypic results showed that 0.94 awexceeded 0.98 aw in AFB1 reduction (2011 and 4280 ng/gm),respectively which agreed with gene expression results. When MaizeMeal Agar used as a medium, aflD expression presented a highsignificant increase at 0.98 aw relating to the NM level of each individualtreatment (P= 0.000 - 0.03) except PFDL+AFL14 which showed nosignificant aflD expression between both levels. aflR expression revealeda high significant reduction (P= 0. 000) caused by 0.98 PFDL+AFL14and 0.98 BSW+AFL14, respectively, while no significant differenceswere observed in the rest of the treatments or control. The HPLC resultsdisplayed a high significant reduction of AFB1 at 0.98 aw (8447 ng/gm)compared to (219000 ng/gm) at NM aw. The biocontrol agents caused ahigh significant reduction for both aflD and aflR expression (P=0. 000 - 0.043) under each individual water activity level while, HPLC resultsshowed non significant low reduction to the AFB1 performed by BSS4and BSW (25160 and 44790 ng/gm), respectively, followed by asignificant increase in AFB1 amount caused by PFMst and PFDL(267600 and 184100 ng/gm), respectively

دراسة بيئية لبعض العناصر الثقيلة في مياه نهر الفرات عند مدينة الناصرية وقابلية مراكمتها من قبل بعض الطحالب الخضر المزرقة ونبات الشمبلان مختبريا == Ecological Study of Some Heavy Metals on The Euphrates river Water at Nassiriyah City and The Ability of Accumulation by Some Blue green Algae and Ceratophyllum demersum L. in the Laboratory

Author name: عبد الوهاب ريسان عيال العبادي
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: plant - aquatic life
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The diversity of phytoplankton were studied and some physical and chemical environmental factors are (Temperature of air and water , pH , electrical conductivity , salinity , dissolved oxygen , alkalinity , hardness and nutrients) of four locations on Euphrates river water at Nassiriyah city in Thi Qar governorate . The first location lies north - west of Nassiriyah city at Al - Sharif region , whereas the second location lies in Al - Mustafaweia region , and the third location lies in Al - Majzarah region , and finally the fourth location lies in Al - Fadlyia city . Samples of water were collected from January to December of the same year of 2014 .The concentrations of some heavy metals are (lead , cobalt and cadmium ) were estimated , in addition to limitation of the efficiency of some algal species and Ceratophyllum demersum L. in bioremediation of their elements on the river water and a limitation of the accumulation sites of the heavy metals on the cells of the algal and plant by using the scanning electron microscope - x - ray . The results revealed clearly a variation on monthly , quarterly and locations of the number properties of the physical and chemical that were studied , the temperature of water ranged between 11.5cہ - 43.6c ہ and air ranged between 15.5cہ - 44.9 cہ , the high temperature at the second location was recorded .The high value of pH 8.62 in the forth locations at April was recorded and the low 7.2 in the third location at July . The concentrations of electrical conductivity were ranged between 3308 - 5773 μs / cm , whereas the concentrations of salinity ranged between 2.11 - 3.69 gm / l , the high concentration on the third location at February was recorded , and the low on the first location at April was recorded , while the concentrations of the dissolved oxygen ranged between 5.40 - 11.70 mg / l , the high concentration on the first location at February was recorded , whereas the low concentration on the third location at August was recorded , also the alkalinity concentrations ranged between 13 - 250 mg / l , the high concentration on the fourth location at March and the low concentration on the third location at July was recorded . From the results , it was show that , the river water was hardship , the hardness concentrations ranged between 850 - 1610 mg / l , the high concentrationaon the third location at January , and the low on the second location at May was recorded .While the concentrations of the nutrients , as nitrate and phosphate , the nitrate concentrations ranged between 13.6 - 41.5 mg / l , the high concentration on the third location at February and the low concentration on the first location at July , whereas the phosphates concentration ranged between 0.83 - 1092 mg / l , the high concentration on the third location on first location at April.Also the diversity of phytoplankton had been studied that were present in water of the river, There were diagnosis 198 species distributed between six classes , the dominance to the Bacillariophyceae as 90 species which belonged to 19 genus were recorded and at the percentage 45.45% of the total numbers of species recorded , followed by the Chlorophyceae 46 species which belonged to 25 genus and at the percentage 23.23% of the total species were that were recorded , followed by Cyanophyceae algae 46 species which were belonged to 15 genus and at the percentage 23.23% and algae of the Euglenophyceae had eight species which were belonged to three genus record and at the percentage 4.04% , with regard to the algae of Xanthophyceae had recorded five types at there belonged to three genus and at the percentage 2.52% and finally the algae of Chrysophyceae which was recorded three types belonged to the same genus , which collectively represent the percentage 1.51% .The study also experienced a record for species of phytoplankton at the first time in Iraq , which were five belonged to four classes , one of them was belonged to Chlorophyceae algae (Trochiscia granulate Prescott.) and the others to the Euglenophyceae(Phacus pyrum (Ehrenb.) Stein and Trachelomonas simillis Stokes) and one species for Chrysophyceae(Mallomonas elliptica (Kisselew) Conrad)and Xanthophyceae algae (Peroniella planctonica G.M.Smith).The concentration of some heavy metals (lead , cobalt and cadmium) in water of the locations was studied by using the flame atomic absorption spectrophotometer , the results were showing that , the concentrations of lead ranged between 4.58 - 29.0 μg / l , as record the high concentration on third location at August and the low on the first location at December , while the concentrations of cobalt were ranged between 4.60 - 23.0 μg / l , as record low concentration was on the first location at December ,bwhile the high concentration on the third location at August , cadmium concentrations ranged between 0.84 - 14.63 μg / l , the low concentration 0.84 μg / l recorded in the first location at December , while the high concentration 14.63 μg/ l in the third location at June .The study included too . a laboratory experiments to demonstrates the efficiency of some species of algae and Ceratophyllum demersum L. on the bioremediation of pollutants in the river water . The results indicates that , there was an increase on the percentage of the removal of elements with the increasing concentrations of elements that were added to water aquarium , reaching the low percentage of biological remove of the lead 95.2% at the treatment 50 mg / l and at the time period 10 days , whereas the biological remove of the high percentage 99.2% at the same treatment during the time period 40 days.As for the cobalt element , the results showing that , there were a gradual increases of the proportion of the removal with the increasing concentrations of cobalt that were added to the water aquarium , and reached to 94.2% at the treatment 50 mg / l at the time period 10 days , whereas the percent for the removal 98.56% at the time period 40 days at the same treatment , whereas the percentage of the vital removing of the element cadmium by the water plant Ceratophyllum demersum was increases in removing from the water aquarium that was 92.6% at the treatment 50 mg / l at the time period 10days , whereas it was 97.40% at the same treatment .As for the algae it had been isolated and diagnoses , the were four species of , blue - greens (Microcystis aeruginosa Kütz. , Nostoc linckia(Roth) Bornet and Thuret , Oscillatoria amoena(ktz.) Gomont and Hapalosiphon aureus West and West , samples were collected from the Euphrates river water on the studied locations . It was purified and propagation of these species on the laboratory for a single culture cell , unialgal culture and axenic culture to test their ability to remove and accumulation of the heavy metals from water. These species of algae were treated salt solution of heavy metals are lead , cobalt and cadmium both in privates and at different concentrations 0.10 , 0.25 , 0.50 , 1.00 and 2.00 mg / l for a period of two weeks . H.aureus was accumulated more of lead 1.724 μg / gm at the treatment 2.00 mg / l , followed by O.amoena which accumulated 1.638 μg / gm and N.linckia accumulatedc1.621 μg / gm and finally M.aeruginosa accumulated 1.579 μg / gm at the same treatment , but when adding the salt solution of cobalt nitrate , the alga O.amoena was accumulated more of cobalt element 1.726 μg / gm at the treatment is 2.00 mg / l , followed by H.aureus as accumulated 1.638 μg / gm , and was N.linckia accumulated 1.535 μg / gm , followed by M.aeruginosa 1.428 μg / gm at the same treatment . When are using the solution of the salt cadmium acetate , the N.linckia was accumulated more of cadmium 1.08 μg / gm , followed by H.aureus which was accumulated 0.63 μg / gm and finally O.amoena accumulated the percentage was reached 0.59 μg / gm at the same treatment .The study was included too the a limitation sites and accumulation of heavy metals within the plant cells and algae that were studied by using technology of scanning electron microscope - x - ray and the use of reagent X - ray EDX with SE2 .The results were showing that , The locations which accumulated heavy elements was in the cytoplasm of the cell . On the above of the foregoing the Ceratophyllum demersum L. showing high efficiency of accumulation of heavy elements inside the cells as compared with the studied algae , the efficiency and ability to increase of the accumulation increases by added of elements and by the increases period of exposure , also algae were revealed a capabilit

حساسية وخصوصية تقنية تفاعل السلسلة المتبلمرة Polymerase Chain Reaction PCR وبعض الطرق المصلية في الكشف عن الاصابت بطفيلي Toxoplasma gondii في النساء المجهضات وازواجهن == Sensitivity and specificity of Polymerase Chain Reaction (PCR) Technique and some Serological methods in the detection of the infection with parasite Toxoplasma gondii in women with recurrent miscarriage and their husban

Author name: فيحاء نوري عبد الحسن
Supervisor name: صبيح هليل المياح | شيماء جبار ريسان
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: Atotal of 300 blood samples were collected from abortifacients women and their husbands who consalted Al Sadr Teaching Hospital in Maysan province and private laboratories in Basra during the period from July 2015 to October 2015, In the same time 50 samples of uninfected males and femals were collected and used as a control groupAtotal of 300 blood samples were collected from abortifacients women and their husbands who consalted Al Sadr Teaching Hospital in Maysan province and private laboratories in Basra during the period from July 2015 to October 2015, In the same time 50 samples of uninfected males and femals were collected and used as a control group . The examination by Enzyme Linked Fluorescent Assay (ELFA) demonstrated that 160 (53.3%) of these samples were positive for toxoplasmosis , at rate of 53.3% , IgG antibody recorded an increase in percentage comparing with IgM and IgG + IgM together , IgG antibody recorded increase in the 138 samples at rate % In PCR technique two genes B1 and Tghsp70 were used to diagnose the same samples for the first time in Iraq and showed highest sensitivity (90.7%) . However age group 31 - 35 years recorded a high percentage of infection (90.5%) . The sensitivity of LAT test for detection of the toxoplasmosis was 80% , ELFA test recorded sensitivity 53.3% and PCR 90.7% with specificity were 60%, 53,3% and 70.7% respectively . Also the concentration of heat shock protein HSP70 in abortifacients of women and their husbands has been estimated , according to concentrations of 20, 40, 80, 160 and 320(ng/ml).II SummaryWhen examined used by ( LAT) test the results showed that males differ fromfemales with a significant difference P≤0.05 , the rate of males were 70% in theconcentration 160ng/ml , But it was observed that women with recurrent abortion, had higher rate 30.8% with high significant difference at the level of probabilityP≤0.01 in the concentration 80ng/ml , compared with males . The results of theage groups showed had highest levels of heat shock protein HSP70 were in theage group (>40) years at rate 66.7% in a concentration 320ng/ml and with highsignificant difference P≤0.01 compared with other age groups, While the agegroup (20 - 25) years had higher rate 11.4% in concentration 20ng/ml comparedwith other age groups , also the age group (26 - 30) years had higher rate 23.3%and 56.7% in concentrations of 80ng/ml and 160ng/ml respectively comparedwith other age groups.The results of estimated heat shock protein for the samples examined by ELFAtest showed that husbands were different from their wives and had higherrate75% with high significant difference P≤0.01 in concentration 160ng/ml .While the ratio of females were 37.5% in the concentration 320ng/ml higher thanthe males with a significant difference P≤0.05 , Also the result showed highsignificant difference P≤0.01 in concentration 160ng/ml and 320ng/ml betweenthe age groups , the age group (35 - 31) years had higher rate 66.7% inconcentration 160ng/ml and the age group (>40) years in concentration320ng/ml had higher rate 66.7%.As well the results of estimated heat shock protein HSP70 of the samplestested by PCR assay in the current study showed a significant difference P≤0.05between males and females , while the ratio of males at rate 59.4% higher thanthe ratio of women in a concentration 160ng/ml , but the rate of women understudy in concentrations 40ng/ml and 80ng/ml were at rate 11.8% and 27.2% ,respectively higher than the males with high significant difference P≤0.0 , onthe other hand no significant difference showed in this study between males andfemales in concentrations 20ng/ml and 320ng/ml , but the results showed thatage group (>40) years had higher rate 66.7% in the concentration 160ng/mlcompared with other age groups . While the age groups (20 - 25) years had higherrate 22.9% in concentration 80ng/ml compared with other age groups . So theage group (36 - 40) years had higher rate 14.3% in concentration 20ng/mlcompared with other age groups .Ministry of Higher Education University of Basrah Sensitivity and specificity of Polymerase Chain Reaction (PCR) Technique and some

دراسة الطفرات الوراثية في جيني TPO وTSHR في محافظة البصرة

Author name: فله عبد الستار
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Plant - Genetics
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Considered the thyroid glands task Deaf which produces important hormones are Tri iodothyrosine T3 and Thyroxin T4 and is responsible for their production a number of genes that encode the enzymes and proteins and the occurrence of any defect in this gene lead to the emergence of disorders of the thyroid work, including the case of palaces and fragments in hyperthyroid through the emergence of different pathological symptoms that indicate the occurrence of one of the cases studied in this research gland disorders at the level of molecular genetics .The study included only females were collected blood samples from 70 patients divided into 35 blood samples of the state of palaces and 35 blood samples for the case of the bulk in hyperthyroid deaf and diabetes endocrine centers of hospitals and ports Fayhaa year and Qurna, as well as from laboratory d. Nizar Mahfouz and represented a group of patients and 20 blood samples from healthy people represented as a set of control and that during the period from April to September 2013. But has been working on a sample of 70 patients (35 blood samples by the state of palaces and 35 blood samples for the case of the bulk gland) to add to the 20 blood samples of control and ages of the two groups and the healthy range (12 - 60 years).DNA was extracted DNA of two and three encoded amplified regions (8, 9 and 14) for the TPO gene and encoded region tenth TSHR gene PCR technology after it was diagnosed and analysis of mutations using the method for Sequences .The results showed the presence of two types of changes in the TPO and TSHR genes , two polymorphism (SNP) and Mutation, in gene156TPO study found hereditary forms c.1117G> T, A372S and c.1194G> C, S398T in the two groups of patients and healthy and not they relate to the occurrence patients palaces gland activity, as the study diagnosed 12 Novel Mutation in exon 8 in 21 patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all mutations diagnosed are mutations bitmap compensatory (substitution) the type of guarantee or Transtion Transversion depending on the nature of the base mutant, five of the mutations are Missense Mutation in 11 infected condition limitations are as follows c.949A>G,M317V , c.1064G>A,R355H, c.1277C>G,A425G , c.1063C>A,R355S and c.1207C>A,L402M and seven silent mutation in 10 patients hypothyroidism c.1062G>A,A354A , c.1050C>A,L350L , c.843C>A,A281A , c.1101G>A,V367V, c.1143C>T,I381I , c.1071G>A,R357R and c.2406C>T,D802D , While the results did not show any difference in the analysis of the genetic sequence of the two exons 9 and 14 at comparable with the genetic sequence of the control group. In this study suggests that there may be a correlation between the occurrence of mutations in a gene TPO and the state hypothyroidism . The results of the current study showed that the gene TSHR has an impact in both cases, and shortcomings in fragments in the activity of the gland where the study found three types of polymorphism (SNP) of the two of them Non - Synonymous type c.2181G> C, E727D and c.1489G> C, A496P and shape The last of the silent type genetic c.1377G> A, A459A, as the results showed that the two forms hereditary c.2181G> C and c.1489G> A have no correlation to happen disorders and are globally registered the shape genetic c.1489G> C, A496P did not score previously has current study for the first time and may have a link to happen one of the disorders depending on the genetic predisposition of the individual . I found the157current study, seven Novel Mutation in exon 10 distributed to five mutations among patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all the mutations diagnosed are mutations bitmap compensatory (substitutive Substitution) to ensure the kind of Transtion or Transversion depending on the nature of the base mutant 'three mutations are Missense Mutation in 3 people with the status of the palaces and the proportion of each mutation them (20%) are as follows c.1330 T>C , Y444H ; c.1424 T>C ,L475P and c.1435 T>C , S479P and two silent mutation in patients hypothyriodism c.1338 G>A, L446L and c.2300 G>A , Untranscrib , While the results recorded and there is only Tafrtin in the case of the bulk in hyperthyroid by (5.71%), one Missense mutation (c.1832 C> A, P610Q) and the other is silent (Silent) (c.2103 C> A, R701R). The present study indicated that the probability of the occurrence of the disease mutations link through the site mutations in specific areas to link hormone catalyst and the interaction between the Receptor and the unity of secondary alpha (G - protein). Results of the current study also showed that mutations identical and disparate factors have an impact on events in the case of deficiencies, genetic gland activity in the TPO and TSHR through defect events and constructed partially or wholly in the function of the protein encoded by genes TPO and TSHR

عزل وتشخيص مركب ايضي ثانوي جديد لكل من الفطرين Fusarium Trichoderma harzianum وsolani المعزولين من السواحل الرسوبية في محافظة البصرة وتقييم فعاليتهما الحياتية == Isolation and Identification of Novel Metabolic Compound From Both Fusarium solani and Trichoderma harzianum Isolated From Sedimentary Coast of Al - Basrah City and Estimate their Activity

Author name: فردوس نوري جعفر
Supervisor name: محمد لطيف علي | نيران جاسم الصالحي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: with the interest of medical and pharmaceutical uses of the natural products from marine fungi , thirty species of fungi belong to ninteen genus were isolated and identified from sea coast sediment of Al - Faw and Al - Seeba .The fungi Acremonium strictium , Geotricum candidum, Fusarium solani ,F.oxysporum , Penicilium sp. ,Trichoderma harzianum exhibited high inhibition activity toward growth of two reference strains of bacteria Escherichia coli (ATCC25922) and Staphylococcus aureus (NCtC 6571). The six selected fungi were grown in liquid culture media PDB at 25°C for two weeks ethyl acetate was used to extract the active compounds from thefermentation liquid media while ethanol was used to extract the active compounds from fungal mycelia .The crud extracts (fermentation and mycelium ) showed different inhibition activity against the pathogenic bacteria , Proteus mirabilis , S. aureus, isolates E. coli, Pseudomonas aeruginosa Klebsella pneumoniae. and pathoginic yeastes Candida albicans, C.tropicals,Cryptococcus neoformans and dermatophytic fungi Trichophyton verrucosum and Microsporium canis . The results revealed that the fermental fungi extracts has more inhibition activity than mycelial fungal extracts.The minimal inhibitory concentration (MIC) of fungal crude extracts were determined against gram positive and gram negative bacteria and pathogenic yeasts and fungi . antagonisum between studied fungi were evaluated , and the fungus T.harzianum has been the more antagonistic effect against other fungi. The toxicity of the fungal crude extracts were tested by using of human RBC at different concentration the result of this experiment showed that all extracts are non toxic except F.oxysporum extract.From all the above results the both F.solni and T.harzianum extracts showed higher activities ,thus these two extracts chosen for further studies. The anticancer activity of both extracts were studied against Hela cell line and the anticancer activity appeared at 250 Mg/ml and 62.5Mg/ml respectivily. The antioxidant activity of extracts were examined by three different methods ,B - carotene ,hydrogen peroxide scavenger and ferrus ion reduction , the results of three methods showed that both fungal extracts have antioxidant behaviours and the T.harzianum extract had more antioxident activity than F.solani extract and the antioxidant activity of both fungal extracts were increased as the concentration of the extracts increase.An optimization of the fungal growth and bioactivity of both fungi were conducted, by using different Nitrogen sources ( Asparagine , Yeast extract , peptone , Ammonium chloride , Sodium nitrate , Ammonium sulphate ) and carbon sources ( Maltose , Galactose , Glucose , Dextrose , Sucrose ,xylose and Starch ) the results elucidated that yeast extract was the best for growth of both fungi on solid and liquid media and high inhibition activity appear with T.harzianum against bacteria isolates , the carbon source starchenriched the growth of F.solani with more activity in inhibition the bacterial growth while the sucrose give high enrichment to the growth of T.harzianum in solid and liquid media with high activity toward bacterial growth. The study also indicates that the beast temperature and pH which gives the higher activity and growth 25°C and pH6 . The effect of different concentration of salinity were studied as well (1%,2%,3%,4%,5%,6% NaCl)and 2% showed the best concentration .The activity of two studied fungal extracts were superior than commercial antibiotic toward bacterial isolates .Preliminary qualitative chemical tests were carried out on the extracts and their chemical nature were identified .TLC technique were used and the chemical analysis showed that F.solani extract consist of two components with constant Rf values, the components marked as A1 and A2 . A1 shows higher activity against bacterial strains while T.harzianum extract consist of one component.The chemical structures of the isolated compound were

التشخيص المظهري والتغاير الوراثي لذباب القرعيات Dacus frontalis Becker ,1922 في محافظتي البصرة وميسان == Morphological identification and genetic variation of cucurbit fly Dacus frontalis Becker ,1922 in Basrah and Missan provinces

Author name: فاطمة قاسم حمدان
Supervisor name: فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study is identified (morphologic and molecular )for the population of cucurbit fly which is a destructive pest of cucumber ,honey melon and cantaloupe in Basrah and Missan .Sample were collected from different region from Basrah province including Qurna , Safwan , Zubair , Khor Al - Zubair . Al - Misharah , Kumate , Kalat Salih, Ali Al - Garbi in Missan province for the period October ,2014 till September 2015.Result of the identification study showed there is only one species infect cucurbits which is Dacus frontalis Becker ,1922,family Tephritidae ,Order Diptera in all studied regions. This species was classified according to femur color of the middle leg ,its color is orange at the apical half with dark black spot at the basal part of the antenna with the presence of basal diagonal line underneath the scutum behind the thorax. female were characterized by the presence of ovipositor at the end of the abdomen and male characterized by present s one line of hair at the third of the abdomen ring .For the sensitivity of the insect fly cucurbits towards our fruits of cucurbits (cucumber and water melon represented by the number of eggs laid down by the female as it appeared no significant difference in the number of eggs laid down by the female in the provinces of Basra, Maysan and was the highest rate of lay eggs on the fruits of cucumbers choice of six eggs and a difference of moral on water option, which amounted to one egg.Molecular study show the kit equipped Bioneer company and named Genomic DNA Extraction kit was highly efficient in DNA Extraction it was later used in the Random Amplified Polymorphic DNA - Polymerase Chain Reaction which is one of the methods used in molecular identification .Used four primers for different sequence s. and electrophoresis results shows multiplied bands of different molecular weight for four different primers for both females and males .The number and molecular weight of bands was calculated manually comparative with DNA marker or ladder then used the number of bands in the following equation to find the genetic similarity and genetic distance between the different districts each primer for males and females .To find genetic similarity used the equation : Sfy=2Nfy/Nf+ Ny and to findgenetic distance used the equation : GD fy=1 - SfyAt the level of first primer for females it was less value of genetic distance is zero and this is evidence that one species and highest value of genetic distance is 0.9 on the second level of the primer for females it was less value of genetic distance is 0.2 and highest value of genetic distance is one at the third primer for females it was less value of genetic distance is 0.3 and highest value of genetic distance is one but the fourth primer for females it was less value of genetic distance is 0.3 and highest value of genetic distance is one .For males at the level of first primer of males it was less value of genetic distance is zero and highest value is 0.8 but the second primer of males it was the less value for genetic distance is 0.8 and on the level of genetic distance is zero on the level of third primer for males it was value of genetic distance is zero and highest value is 0.8 and fourth of males it was the less value of genetic distance is zero and highest value of genetic distance is 0.8

دراسة تصنيفية وبيئية لبعض انواع عائلة ذباب الخيل Diptera : Tabanidae ودورها في نقل طفيلي Trypanosoma evansi في محافظة البصرة

Author name: علاء ناظم حاتم علي
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Zoology - Insects
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تقدير بعض العوامل البيئية والبكتريولوجية لمياه الصرف الصحي في محافظة البصرة / العراق == Assessment of some environmental and bacteriological factors of Wastewater in Basra Province, Iraq

Author name: سهى محمد علي محمد سعيد المظفر
Supervisor name: منال محمد اكبر | ميثم ايوب عبد القادر
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير مياه الصرف الصحي على المحتوى البكتيري في محطة حمدان لمعالجة مياه الصرف الصحي /البصرة حيث تستلم المحطة مياه المجاري المنقولة عبر شبكة من الانابيب التي تستلم مياه المجاري من50% من مناطق مدينة البصرة. اختبرت ثلاث مواقع لاخذ العينات هي المحطة الاولى حوض التجميع الخارجي والمحطة الثانية حوض الترسيب الابتدائي اما المحطة الثالثة فهي المياه الخارجة من المحطة والتي تصب في شط البصرة.وشملت الدراسة ايضا قياس بعض الخصائص الفيزيائية والكيميائية لمياه المجاري وهي (الاس الهيدروجيني pH ودرجة الحرارة Temperature والملوحة Salinity والمتطلب الحيوي للاوكسجين BOD5 والفوسفات والمواد الصلبة العالقة الكلية TSS والمواد الصلبة الذائبة الكلية TDS والنتريت NO2 والنترات NO3) خلال الفترة من كانون الاول2014/ ولغاية ايار2015/.ويتضح من نتائج هذه الدراسة ان المتطلب الحيوي للاوكسجين BOD5 هو عامل مهم وله علاقة باعداد البكتريا الكلية الموجودة في مياه الصرف الصحي.كما شملت حساب كثافة المجاميع البكتيرية المتواجدة في مياه المحطات.ولحساب العدد الكلي للمستعمرات البكتيرية اخذت عينات مياه الصرف الصحي وزرعت على اوساط عامة وانتخابية وتفريقية مختلفة استخدم وسط الاكار المغذي Nutrient Agarكما واستخدم وسط المانيتول الملحي الانتخابي Mannitol Salt Agarلعزل المكورات العنقودية الذهبية Staphylococcusaureus ووسط اكار الماكونكي MacConkey Agar لعزل بكتريا القولون البرازية Escherichiacoli ووسط AgarXLD الانتخابي التفريقي (Xylose lysine deoxycholate Agar ) لعزل انواع البكتريا التابعة لجنس السالمونيلا Salmonella.تم ايضا دراسة حساسية العزلات تجاه المضادات الحيوية باستخدام طريقة الانتشار عبر الاقراص Disc Diffusion Test حسب طريقة كربي باور Kirby Bauer Method. ان دراسة حساسية عزلات E. coli توصلت الى ان العزلات البكتيرية ابدت نسب مقاومة عالية لاغلب المضادات الحيوية المختبرة في هذه الدراسة ، كذلك ابدت المكورات العنقودية الذهبية مقاومة عالية لاغلب المضادات الحيوية في حين كانت حساسة لكل من كلورامفينيكول (C) وسيبروفلوكساسين (CIP) ونورفلوكساسين (NOR) وكانامايسين (K). كانت عزلات السالمونيلا متباينة في حساسيتها تجاه المضادات الحيوية المختبرة في هذه الدراسة لكن ابدت عدم مقاومة او مقاومة واطئة للمضاد الحيوي CIP وNOR . ابدت المجاميع البكتيرية المختلفة قيد الدراسة انماط مقاومة مختلفة تجاه المضادات الحيوية اذ ابدت انماط مقاومة مختلفة. وكذلك ابدت العزلات البكتيرية المعزولة من مياه الصرف الصحي مستويات مختلفة من التحمل لتراكيز المعادن الثقيلة ،فيما يخص عزلات E. coliابدت العزلات مستوى عال من التحمل للنيكل بلغ 64% من العزلات كان MIC لها 4mg/Lوللكادميوم فان نسبة 93% من العزلات كان MICلها 0.8mg/L. اما بالنسبة للمكورات العنقودية الذهبية فان 89% من العزلات كان MIC لها 2mg/L لعنصر النيكلوبالنسبة للكادميوم فان 33% من العزلات كان MIC لها 0.4mg/L .وبالنسبة للسالمونيلا كان تحمل 100% من العزلات لعنصر النيكل هو عند 2mg/L في حين كان 50% من العزلات MIC لها 4mg/L كحد اقصى لعنصر الكادميوم. | The present study has been conducted to detect the effect of different parameters of sewage on microbial content at Hammdan for wastewater treatment in Basra city. The station receive sewage transported via net of pipes from different parts of Basra city .Three sites in the station for taking samples ,the first site is the external collection basin, the second site is primary sedimentation basin, the third site is water output that carry the water after treatment to flow at Shatt al Basra. Some characteristics of sewage were measured, these include pH, Temperature, Salinity, BOD5, TDS, TSS, Phosphate, NO2 and NO3 from period December/2014 to May/2015. In this study it is clear that BOD5 is an important factor related to total bacterial count in sewage. The bacteriological study of sewage has been done including measurement of total count of bacteria isolation and counting of Escherichia coli, Staphylococcus aureus and Salmonella spp. From different stations selective media is used for isolation including Nutrient Agar , MacConkey Agar , Mannitol salt Agar and XLD Agar . The antibiotic susceptibility of the isolates towards selected antibiotics have been studied using disc diffusion test. In the present study E. coli isolates showed high percentage of resistance to most of antibiotics. Staphylococcus aureus also showed high percentage resistance to most of antibiotics studied and found susceptibility to Chloramphenicol, Ciprofloxacin and Kanamycin. Variable suceptibility to tested antibiotics in the present study has been shown by Salmonella spp., Salmonella spp. were susceptible or show low resistance to Ciprofloxacin and Norfloxacin .Different Antibiotics Resistance Profiles has been shown by different bacteria groups studies. The bacterial isolates from sewage in this study have shown different levels of tolerance to different heavy metals (Nickel and Cadmium). E. coli tolerate high concentration for Nickel (%64) and for Cadmium (%93). High tolerance level for Cadmium has been shown for Salmonella spp (%50).

خصائص خمائر البيئة النهرية لمحافظة البصرة وقابلية بعضها على المعالجة الحيوية للعناصر الثقيلة والهيدروكربونات النفطية == Characterization of yeasts in river habitat in Basrah Province and an ability of some of them for Bioremediation of the heavy metals and oil hydro carbonate

Author name: سناء قاسم بدر
Supervisor name: باسل عبد الزهرة عباس
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تقييم حالة النباتات المائية الغاطسة في بعض مناطق هور شرق الحمار وشط العرب جنوبي العراق باستخدام بعض دلائل التنوع الاحيائي == Assessment of the Status of Submerged Aquatic Plants in Some Areas of East Hammar Marsh and Shatt Al - Arab River Southern Iraq Using Some Biodiversity Indices

Author name: سما عباس عبد المجيد الزيدي
Supervisor name: دنيا علي حسين العباوي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted to assess the state of submerged aquatic plants in some areas of East Hammar marsh and Shatt al - Arab River, as measured by some of the chemical and physical properties of water and sediment in six stations, three of which are located in East Hammar marsh (Nagara, Sallal and Almshab) and the other three are located in the Shatt al - Arab (the island Muhammadiyah, Salhiya and Mhella). Environmental surveys of submerged aquatic plants were done and registered their presence, their vegetation cover and biomass as well as the biodiversity indices (diversity and evenness, and dominance) for 12 months from October 2015 until September 2016.The air temperature in the East Hammar marsh ranged between 19 - 45.3 C, water temperature 14.3 - 32.4C , transparency between 20 - 100cm , Turbidity between 20.9 - 65.3 NTU, depth of 50 - 160 cm and dissolved oxygen between 5.4 - 6.9 mg / L, pH between 7.6 - 8.6 , electrical conductivity between 3.8 - 12.5 mS / cm, nitrates between102.23 - 549.64 μg/L, effective phosphorus between 4.15 - 9.6 μg/L . As for the Shatt al - Arab River values ranged : air temperature between 18 - 48 C , water temperature was between 14.2 - 32.3 , Transparency between 23 - 90 cm, turbidity between 12.8 - 68.3 NTU , depth of 60 - 200 cm , dissolved oxygen between 5.4 - 7.9 mg / L , pH between 7.5 - 8.51 , electrical conductivity between 2.8 - 12.4 mS / cm , nitrates between 106.17 - 569.42 μg/L, effective phosphorus between 4.93 - 9.13 μg /L .BpH and electrical conductivity values of the sediments in the Shatt al - Arab and East Hammar marsh were reached 7.7 - 8.3 and 1.08 - 2.91 mS / cm and 7.5 - 8.4 and 1.12 to 3.05 mS / cm respectively, effective phosphorus values in sediment ranged in sediment of East Hammar marsh between 5.69 - 42.51μg/L, While in Shatt al - Arab fluctuated between 5.12 - 40.3 μg/L, and the value of the organic matter in the Shatt al - Arab were 5.6 to 14.5%, while in the East Hammar marsh reached 7.4 - 15%. The sediments are similar texture in the Shatt al - Arab and East Hammar marsh (Silty clay).The presence of three species were recorded in the Shatt al - Arab River included Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus, while five species in the East Hammar marsh Ceratophyllum demersum, Myriophyllun spicatum , Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus.As calculated biodiversity evidence was that East Hammar marsh was more diverse than the Shatt al - Arab, while dominancy was clear in the Shatt al - Arab, compared with East Hammar marsh.This study showed that the sudden rise in salinity values as well as increased nutrients led to degradation of water quality, which was reflected on the presence and vegetation cover of submerged aquatic plants as well as biodiversity resulting from the presence of Tilapia and other fish that use submerged aquatic plants as food as well as the human factor.

تاثير هرمون الثايروكسين على بعض المعايير الوظيفية للدم وبعض الجينات المنظمة لتمايز النسيج العظمي في الفئران المختبريةmusculus L. Mus. == Effect of Thyroxine hormone on some of physiological parameters of blood and some regular genes of bone differentiation in Laboratory Mice Mus musculus L.

Author name: سلمى سعيد عباس
Supervisor name: سامي جبر المالكي | علي عبد اللطيف العلي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير هرمون الثايروكسين على تمايز العظم وبعض المعايير الفسلجية والتي شملت المعايير الدموية والهرمونات المحرضة للقند واوزان واطوال الاجنة واوزان المواليد في الفئران المختبرية البيضاء Mus musculus L.,اذ قسمت اناث الفئران المختبرية في الدراسة الحالية على مجموعتين هما : المجموعة الاولى الرئيسية : لدراسة تاثير هرمون الثايروكسين L.Thyroxine hormone على المعايير الدموية للاناث الحوامل وغير الحوامل وعلى تمايز العظم في الاجنة(باستخدام طريقة تحضير المقاطع النسجية الاعتيادية للاجنة والطريقة الجزيئية باستخلاص المادة الوراثية (RNA) من الاجنة وباستخدام تقنية النسخ العكسي RT - PCR والبلمرة الكمية qRT - PCR ومعرفة تاثيره على اوزان المواليد والاجنة, وتالفت من 90 انثى بالغة بعمر(10 - 12)اسبوع وقسمت على مجموعتين ثانوية ,و هي مجموعة السيطرة والتي حقنت حيواناتها بـ 0.1 مل من المحلول الفسيولوجي0.9% ولمدة 10 ايام متتالية.بينما المجموعة المعاملة حقنت حيواناتها بـ0.1 مل من هرمون الثايروكسين وبجرعة مقدارها 0.5 ملغم /كغم/يوم ولمدة 10 ايام يوميا بعدها شرحت 5 اناث من كلا المجموعتين ,و زوجت 40 انثى المتبقية ولكلا المجموعتين مع الاستمرار بالحقن لحين تشريح الاناث واستخراج الاجنة خلال فترات عمرية متباينة (9و10و11و12و13و14و15) يوم.اما المجموعة الثانية الرئيسية خصصت لدراسة تاثير هرمون الثايروكسين على الهرمونات المحرضة للقند وهرمونات الدرقية وانزيم Alkaline phosphatase.وتتالف من 15 انثى وقسمت على ثلاث مجاميع ثانوية تضم كل منها (5) اناث, وهذه المجاميع هي : مجموعة السيطرة وحقنت بـ0.1 مل من المحلول الفسيولوجي 0.9%.ومجموعة المعاملة الاولى وحقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 15 يوم للاناث العذارى قبل التزاوج.ومجموعة المعاملة الثانية حقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 30 يوم . اظهرت نتائج الدراسة الحالية ان المعايير الدموية قد تباينت قيمها خلال الفترات الزمنية وللمجموعات ضمن المدى الطبيعي عدا في حالات قليلة ,اذ وجد هناك انخفاض معنوي في اعداد خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية واعداد كريات الدم الحمر,وكذلك قيم مستوى كمية الهيموكلوبينHb والنسب المئوية لحجم الدم المضغوط PCV(HCT)% ومعدل كمية الهيموكلوبين MCHومعدل تركيز الهيموكلوبينMCHC ومعدل حجم كريات الدم الحمرMCV والصفيحات الدموية PLT في فترات متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وبينت النتائج وجود زيادة معنوية في خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية, ومعدل تركيز الهيموكلوبينMCHC والنسبة المئوية لحجم الدم المضغوط %HCT ومعدل حجم كريات الدم الحمر MCVو الصفيحات الدميةPLT في مدد متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وسجلت نتائج الدراسة الحالية حصول ارتفاع معنوي في قيم هرموني T4,T3في الحيوانات المعاملة مع انخفاض معنوي في تركيز هرمون TSHفي الاناث المعاملة مقارنة مع السيطرة,و اوضحت النتائج ايضا انخفاضا معنويا في مستوى الهرمون اللوتيني LH وهرمون الحليب وللمجموعتين(15و30) يوم وزيادة معنوية في الهرمون المحفز للجريبات FSHللمجموعة الثانية(30 يوما) ,مقارنة مع مجموعة السيطرة, ولايوجد تاثير معنوي لهرمون الثايروكسين على هرمون الشحمون الخصوي ولنفس الفترتين مقارنة مع مجموعة السيطرة, وحصلت زيادة معنوية في مستوى انزيم الفوسفاتيز القاعدي بعد 30 يوما من الحقن بهرمون الثايروكسين مقارنة مع مجموعة السيطرة ,وحصول انخفاض في اوزان الاجنة بعمر 15يوما والمواليد للاناث المعاملة مقارنة مع مجموعة السيطرة , وبينت الدراسة زيادة معنوية في اطوال الاجنة المعاملة بعمر 9 و10و11و15يوما مقارنة مع مجموعة السيطرة. اما الدراسة النسجية فقد بينت ان الهيكل العظمي في الاجنة بعمر 9و10و11 يوما عبارة عن بداءات اعضاء تتكون من نسيج ميزنكيمي,ولم تسجل الدراسة النسجية وجود فرق بين مجموعة السيطرة والمعاملة في تمايز العظم .وظهرت بداية التعظم الغشائي والغضروفي في الاجنة بعمر12يوما ,اذ لوحظ بداية تمايز الخلايا الميزنكيمية الى ارومات عظمية,مع وجود القليل من المادة البينية الشبيهة بالعظم بين الارومات العظمية في منطقة الفك السفلي قرب غضروف ميكل ,والفك العلوي والعظم الحنكي واللامي وغضاريف الاضلاع ,اما في الاجنة بعمر13 و14و15يوما فقد ازدادت عمليات تمايز العظم بنوعية ,فاضافة الى النمو الذي حصل في العظام السابقة في تكوينها فقد ظهرت عظام جديدة في مناطق اخرى من هيكل الجنين تمثلت بعظام الصفيحة القاعدية والفخذ وحزام الكتف وحزام الحوض والاضلاع ,اما النتائج الجزيئية الخاصة بتمايز النسيج العظمي في اجنة الفئران المختبرية فقد اشارت الى التعبير الجيني لبعض جينات النسيج العظمي وتشمل Runx2وOSX وOsteo(OSC) وSialo(BSP) والتي بلغت ذروتها في مرحلة (13) يوم. ان لهرمون الثايروكسين تاثير على التعبير الجيني لبعض الجينات التي ترتبط مع التمايز النسجي للعظم اذ يؤدي هذا الى التغير في التعبير الجيني زيادة احيانا وانخفاضا خلال المدد العمرية المختلفة للاجنة .نستنتج من الدراسة الحالية ان زيادة مستوى هرمون الثايروكسين يسبب اضطراب في المعايير الدموية والتعبير الجيني لجينات العظم وانخفاض مستوى الهرمونات المحرضة للقند (LH وProlactin) وزيادة مستوى الهرمون المحفز للجريبات, وتعد هذه الدراسة الاولى على مستوى جامعة البصرة | the present study was performed to know the effect of thyroxine hormone on differentiation of bone tissue and some of physiological parameters, which included blood parameters and Gonadotropine hormones and Weights and Lengths and birth weight of mus musculus L.the females are divided into two groups ;the main first group which included 90 adult females (10 - 12)week, that subjected to study effect of thyroxine hormone on blood parameters of pregnant , nonpregnant females ,and bone differentiation of embryos (by using tissue sections method and molecular technique by RT PCR , and qRT PCR), and also known it is effect on weight of fetus and newborns; this group was also subdivided into two secondary subgroup : - control group ; was injected with 0.1 ml of normal saline (0.9%) for 10 consecutive days ,and treated group; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 10 consecutive days ,after that, 5 females from both groups were anatomized,while 40 females were mated of the both groups with continuous of injection until the females were subjected to anatomical practices and then the the embryo were taken during varied peroids (9,10,11,12,13,14,15) day. The main second group which included 15 females ,was selected to study the effect of thyroxine hormone on ;sexual hormone ,thyroid hormone and alkaline phosphatase . this group was divided into another 3 subgroups,one regarded as control group which ; was injected with 0.1 ml of normal saline (0.9%) for 15 consecutive days.and second the first treated group (T1) ; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 15 consecutive days ,and without mating of the females,and the second treated group ; (T2);was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 30 consecutive days and also without mating of the females. Results of the present study were shown that the blood parameters were varied in value between periods and groups (decreasing and increasing among normal value ) with exception in few cases , significant decrease of W.B.C , the granular cells ,monocytes, lymphocytes ,lymphocytes percentage ,granulocytes percentage and monocytes percentage during different periods of pregnancy in the treated animals compared with control , and was decreased RBC ,Hb ,HCT percentage ,MCH ,MHCH ,MCV, and PLT in varied periods , and we noticed a significant increase in WBC ,granular cells ,monocytes ,lymphocytes , granulocytes percentage , monocytes percentage , MCHC ,HCT percentage,MCV, and PLT in varied periods of pregnancy in the treated animals compared with control. Our study was exhibited significant increase in levels of T3 and T4 hormones of the treated animals compared with control, with significant decrease in TSH of treated animals , also significant decrease in LH and prolactin H and for groups(15 and 30 days) ,and significant increasing in FSH of second group (30 day) ,when compared with the control , and there was no effect of thyroxine on TEST H level and for 2 periods(15 and 30) , significant increasing in alkaline pho after 30 days from injection with TH H , decreased of body weight of embryos (15 days) and newborns of the treated females ,the current study showed significant increase of lengths of treated embryos (9,10,11,15 days). The histological study was explained the skeletal tissue ossification of embryos during (9,10,11)day post gestation was primitive organs of mesenchymal tissue and The histological study was not show asignificant differences of bone differentiation between treated and control groups,and intramemberaneous ossification was appeared of embryos at 12 days ,in this stage the begining of differentiation of mesenchyme cells was appeared into osteoblast , with a little of matrix like bone between osteoblasts in Mandible near the meckle cartilage , Maxilla, Palatine,and Hyoid, and endochondral ossification as in Ribs, where in embryos (13,14,and 15) The bone differentiation processes were increased of bone (the intramembranous ossification and endochondral ossification),in addition to the growth of previously formed bones,also new bones were appeared in different areas in embryo skeleton like Basal plate,femur,Scapula girdle , pelvis girdle and the Ribs . In other hand, the molecular results of bone differentiation in embryo ,were exhibited peak of expression of some of the genes of bone tissue (Runx2 ,OSX, Osteo(OSC) ,and Sialo(BSP) ) at 13 days of embryo age, also the thyroxine hormone was effected in expression of some the bone genes that included disturbanceas like increasing of expression during peroids and decreasing in other peroids of embryos life. We concluded from the present study that the increase of thyroxine hormone level caused disturbances of blood parameters and gene expression of the bone genes & decreasing of the level of LH & prolactin hormones and increase the level of FSH. This is the first study on the Basrah University

تقييم فعالية عقاري Nandrolone decanuate وAlbendazole للاصابات المختلفة بداء الاكياس العدرية في الفئران المختبرية سلالة Balb/c == Evaluate the effectiveness of a drug Nandrolone decanuate and Albendazole for injuries the different infections by hydatid cysts in mice Balb/c

Author name: سعيد فاضل مناتي الحمداني
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: تبنت الدراسة الحالية استعمال طرق مختلفة لاحداث الاصابة برؤيسات الاولية Protoscolex المستخرجة من الطور اليرقي ( الاكياس العدرية ) لطفيلي Echinococcus granulosus وهذه الطرق هي الحقن الوريدي والجنبي وتحت الجلد وكذلك عن طريق الغشاء البريتوني . اظهرت الدراسة حدوث الاصابة وتطور الاكياس العدرية في كل الطرق اعلاه . كذلك تم استعمال طريقة الحقن في اللفائفي والتجريع عن طريق الفم لكن لم تظهر الاصابة المستخدمة بهذه الطرق اي تطور للاكياس العدرية في حيواتات التجربة . كذلك حقن 650 رؤيس اولي في جميع طرق الحقن وتبين حدوث الاصابة في نفس مواقع الحقن بطريقة الحقن تحت الجلد والحقن الجنبي وعن طريق الغشاء البريتوني. ولكن طريقة الحقن الوريدي اظهرت حدوث الاصابة وتطور الاكياس العدرية في اماكن مختلفة من جسم الحيوان المختبري ويكون الكيس العدري متموضعا بين نسيج العضو المصاب في حين الحقن عن طريق الجنب وتحت الجلد والغشاء البريتوني يكون الاكياس العدرية المتطورة ملتصقة على سطح العضو المصاب . كذلك اثبتت الدراسة وجود اختلاف في الوان الاكياس العدرية المتكونه وحسب طريقة الحقن. استـــــعمل في هذه الدراســــــــة عقار Albendazole وعــــــقار Nandrolone decanuate كعقارات علاجات للاصابة المستعملة وتبين ان لها تاثير ادى الى موت الرؤيسات الاولية بتقدم فترة الاصابة واثبتت الدراسة ان عقار Nandrolone اكثر كفاءة من عقار البندازول وذلك من خلال الاختلافات في النسبة المئوية لموت الرؤيسات الاولية وكذلك بسبب هذا العقارشوه في شكل الرؤيسات وطريقة ابعاجها اذ كان الانبعاج الى الخارج في حين كان الانبعاج الى الداخل في الاصابات المعالجة بعقار البندازول . | The present study showed various methods for the infection by the protoscolex obtained from the larval phase of the Echinococcus granulosus . which involved the venus,pleural,subcutaneous and peritoneal injection . In all injection method 650 protoscolex were injected ,It was found that no infection happen in the same location to the injection which resulted by the subcutaneous,pleural and pretonal injections. On the contrary to thatresulted by venous injection in wich the injection occurred in varoes sites of the lab animals body ,and the hydatid cysts were pervasine with in the diseased infected organ tissues. Wile that resulted by pertonial ,subcutaneous and hered the surface of the organ. The colors of the hydrated cysts were also varied according to the infection methods.Albendazole and Nandrolone were used as treatments against the induced infections and it was found that Nandrolone was more efficient than albendazole,That was observed by the rates of the protoscolex caused by the action of the drugs and desmorphorphology and invagination outward while in albendazole was inward

دراسة مقارنة لتاثير هرمون النمو في عضلات وCyprinus مناسل سمكتي الكارب الاعتيادي والبلطي احمر البطن carpio (L. 1758) Tilapia zillii (Gervais, 1848 == A Comparative Study to the Effect of Growth Hormone on Muscles and Gonads of Carp Cyprinus carpio (L. 1758) and Tilapia Tilapia zillii (Gervais, 1848)

Author name: سعد محمد صالح عبد الصمد
Supervisor name: عقيل جميل منصور | عبد الكريم طاهر يسر
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study, the effect of human growth hormone on the muscles and gonads of common carp Cyprinus carpio (L. 1758) and red belly tilapia Tilapia zillii (Gervais, 1848) was studied by : - 1 - Studying the effect of human growth hormone on the growth of the two studied species by intraperitoneal (IP) injection of three replicates of control with 120 μg of normal saline / g Body weight and two doses (low and high) with 60 and 120 μg of human growth hormone / g Body weight respectively for both species, then changes were followed up in each treatment after every two weeks of injection by : - a - Pursue histological changes in the muscular tissue by using Cell Profiler software for the first time in Iraq. b - Pursue changes in chemical components ratios of body muscles.c - Measuring of specific growth rate and length - weight relationship for fishes.2 - Studying of human growth hormone effect on the gonads of fishes (adult males and females) with 60 males for each species and a same number of females, all of them injected either with low or high dose with 60 or 120 μg of human growth hormone / g Body weight respectively for both species or with 120 μg of normal saline / g Body weight in fishes of control. This experiment had achieved in a month.3 - Knowing the direct effect of human growth hormone on the follicles by implementing an in vitro experiment with the follicles of carp which implanted in basic salt solution (BBS) then growth hormone was added to the test tubes with two concentrations 5 μg/ ml and 10 μg/ ml, the control follicles were implanted in normal saline. The results of the study revealed that the maximum rate of percentages of the muscle fibers with area less than 314 μm2 for region R1(Dorsal side) was 88.29 % in carp in high dose treatment after the eighth week of injection, while it was 88.00 % for tilapia in high dose treatment after the eighth week of injection, too.In the region R2 (in tail) the maximum rate of percentages of muscle fibers with area less than 314 μm2 was recorded after the eighth week from the beginning of injection was 73.3553 % for carp in high dose treatment and 85.935% for tilapia in high dose treatment.The results showed that 1.00984 and 1.000074 were the highest and the lowest values of condition factor for carp respectively. For tilapia those were 1.009993 and 1.000151 the highest and the lowest values of it respectively.The rate of specific growth rate for length was 0.3616 and 0.44866 cm/day% for carp and tilapia respectively. While the specific growth rates for weight were higher than the comparable one for length and were 1.3489 g/day% for carp and 1.6085 g/day% for tilapia.The results exhibited that the rates of protein percentages for both species in the all treatments in general tend to be higher with progressing of experimental time. Muscle water content percentage rate varied with difference of dose and experimental time. The maximum rate of fat percentage in both species was in control treatment after two weeks of hormone administration.The highest rate of carp male Gonadosomatic index was 6.55% in high dose treatment after the third week of injection, as for tilapia males was 6.103% in low dose treatment after the third week of injection, while the lowest value for this index was recorded after the first week of injection in high dose treatment which as 2.81 and 2.89% for carp and tilapia respectively. For females the highest rate of Gonadosomatic index was in high dose treatment after the third week of injection for both species which was 11.001 and 6.0624% for carp and tilapia respectively. As for the lowest rate of carp female Gonadosomatic index was recorded in control treatment after the first week of injection it was 5.54%, while the lowest rate of this index for tilapia females was 2.99% in low dose treatment after the first week of injection.In the experiment of direct effect of this hormone on the follicles of carp it was found that the highest value of polarization index was 4.12 which recorded in high dose treatment after 48 hours of incubation while it reached the lowest value which was 3.031 in high dose treatment after 24 hours ofincubation.

دراسة تشخيصية مظهرية وجزيئية للشريطيات المتطفلة على نورس مستدق المنقار Larus genei والكرسوع Himantopus himantopus شمال محافظة البصرة == Diagnostic, Morphological and Molecular Studies on some cestodes parasitized on Larus genei and Himantopus himantopus , North of Basrah province

Author name: سحر محمد علي محمد سعيد المظفر
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: During the period from August 2015 to July 2016, fifty three aquatic birds of Larus genei (slender - billed Gull) family Laridae and 52 of Himantopus himantopus family Recurvirostridae were collected from Al - masehab marsh north of Basrah Province.The current study was focused on these two birds due to its economic importance to the local people of the study area. These two birds were found infected with 10 species of cestodes. Echinocotyle longirostris and Infula burhini were recorded for the first time in Iraq. Larus genei was considered as a new host of Wardium fusa.The isolated cestodes were described and compared with other recorded species at different parts of Iraq and the world.The percentage and the mean intensity of infection of these 10 species of cestodes were counted monthly and seasonly together with single, double and trible infection during the whole year. The main percentage infection was reached to 95.2% and the main intensity was 13.49. In L. genei the percentage and mean intensity were 90.5% and 15.4 recepectively, while for H. himantopus were 100% and 11.8 recpectively. In L. genei the high percentage infection was found in August 2015 and September , November, and December 2016, and during March, April, May, June and July for H. himantopus. A significant difference was recorded between the percentage and the mean intensity of infection.Random Amplified Polymorphic DNA (RAPD) technique with random 6 primers was used to distinguish between Infula burhini and Acoleus vaginatus wich isolated from the bird H. himantopus due to the morphological similarities.The Scanning Electron Microscope (Pharmacy College, Basrah University) was used to photograph the scolex of A. vaginatus and I. burhini.

تشخيص وحياتية حشرة مع تصنيف الديدان الخيطية المتطفلة عليهافي محافظة ميسان == Diagnosis and life cycle insect Gryllotalpa gryllotalpa (L.) with a taxonomy parasitic nematodes in the province of Missan

Author name: سجى عماد نعيم الساعدي
Supervisor name: باسم هاشم عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: It was collected 318 mole cricket insects randomly from four regions in Missan including AL Kahala district by 191 insects, AL Mishraah by 62 insects, Kalat Salih by 61 insects and New Hay AL Hussein ( Centre of the city) by four insects. The period was from September 2013 to August 2014. It was diagnosed one species of mole crickets which is G. gryllotalpa decended from Gryllotalpidae families because of its essential charact ristics such as the general form of the insect, shape of the wings and legs, and its site and colour. In addition, there is a large lobe in the front legs of the insect and there one two species of veins in the front wings of the male used to attract females for mating. The life cycle of the insect was studied in the laboratory. Itstransformation is characterized by being imperfect progress, on egg, then a nymph and then an adult. The female lays about 100 eggs oval - shaped and grey in a room in the soil. The period of preegg - laying is approximately 100 days and the percentage of hatchling is 74% . The females remain alive after one day egg - laying is completed while the males remain 13 days after egg - laying. The eggs hatch into nymphs which pass stages of life development andit takes 115 day - Newly hatched nymphs are transparent and grey, its length is 10 mm with antennas and small cerci which are without wings. Wings begin to appear in the sixth stage of life. Then nymphs develop into brown adults with a large filled body. Its length is about 40 mm and all parts of the insect continues to grow in proportion to the size of the insect.After examining these insect, it showed that they are infected with seven species of nematode parasites. They are Binema ornate, Binema korsakowi, Binema anulinervus, Cameronia triovata, Cameronia multiovata, Mirzaiella asiatica, Grylophilla skrjabini. The Prevalence of those nematodes are 18%, 13%, 1%, 2%, 1.0%, 22% and 25.6% respectively. while the mean intensity of infection was 1.5, 1.6, 3.5, 4.5, 8, 1.5,2.15 respectively.Four of these species of nematodes were considered as new record in Iraq, These are : Binema anulinervus : Most important feature in the shape of the high - tail and long that look like a flagella.Mirzaiella asiatica : Featuring male this form of the parasite by the short and broad and meandering tail. The female is characterized by a length of the muscle of the esophagus and the existence of the tapes curled at his side. Cameronia triovata : The most important feature of this parasite egg shaped oval that is flat from one side. C. multiovata : What also distinguishes this form of the parasite is a large and long oval egg.Nematodes were a taxonomically quoited and illustrated.The species B. ornate and G. skrjabini appear repeatedly over 10 months followed by the parasite B. korsakowi for eight months then followed by M. asiatica for seven months, as for the parasites B. anulinervus, C. triovata, C. multiovata for two months

دراسة مظهرية وجزيئية بتقنية RAPD لبعض الاصناف الغيبانية لنخيل التمر في البصرة == Morphological and Molecular Study by RAPD Technique for Some Un Known (Ghaibany)Cultivars date - palme In Basrah

Author name: زينب شعلان عبد الرزاق علي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية اثناء الموسم الزراعي للعام 2015م على احد عشر صنفا بذريا (غيباني) لنخيل التمر Phoenix dactylifera L. النامية في البصرة وهي اصناف جديدة تسجل لاول مرة, درست ثمان وعشرون صفة من صفات النمو الخضري, وست وعشرون صفة من الصفات الثمرية كما. درست صفات البذور, فتبين ان الاصناف المدروسة اختلفت في الخواص الخضرية والثمرية, اذ اظهرت الدراسة ان كلا الصنفين نبوي والصنف نجمة تميزا بشكل التاج الشبه منفتح في حين كانت الاصناف الباقية مضمومة التاج. وكذلك انفرد الصنف نجمة عن باقي الاصناف بلون الاوراق الاخضر الشمعي. وتراوح معدل طول الورقة من 2.68 م في صنف الوردية الى 4.35م في الصنف نمروشة, كما تراوح معدل طول منطقة الاشواك من 56 سم في صنف دكلة عبد الى 160سم في الصنف ام اللسين. وتباينت الاصناف في معدل عدد الاشواك فكان ما بين 18 شوكة في الصنف وردية الى 47 شوكة في الصنف ام اللسين. واختلفت الاصناف بنمط ترتيب الاشواك على المحور الوسطي فقد انفرد الصنف برماني بنمط توزيع الاشواك الثنائي وامتاز الصنف وردية بالنمط المفرد الثنائي في حين كان النمط المفرد هو السائد في الاصناف الباقية, واتضح من الدراسة ان النسبة المئوية لطول منطقة الاشواك الى طول الورقة الكلي هي من الصفات التي تتسم بالثبات دونا عن باقي الصفات الخضرية. اما بالنسبة للخواص الثمرية فقد تراوح معدل طول العرجون للاصناف المدروسة مابين 66 سم في الصنف رحماني الى 152سم في الصنف برماني والذي يعد من العراجين الطويلة, وانفرد الصنف دكلة داود بلون العرجون الاصفر المائل للوردي اما الصنفين برماني ونجمة فقد كان لون العراجين فيهما اخضر في حين كان لون العراجين في الصنفين وردية ونبوي اصفر يميل للاخضرار واصفر في باقي الاصناف. اما الخواص الفيزيائية المدروسة للثمار فقد سجل الصنف دكل داود اقل المعدلات في حجم الثمرة في مرحلتي الخلال والتمر والتي كانت 5سم3 و2.6سم3 على التوالي, وامتاز الصنف الزريجي بكبر حجم ثماره لكلا المرحلتين الخلال والتمر 17سم3 و10.24سم3 على التوالي, وتبين ان طعم الخلال في الاصناف وردية ودكلة عبد والصنف رحماني والصنف زريجي عفصي اما الاصناف الباقية فهي حلوة المذاق. وامتاز الصنف نجمة بكونه مبكر جدا في موعد النضج وكانت الاصناف الباقية مبكرة النضج في حين كان الصنف برماني متوسط النضج, وامتازت اصناف الدراسة بكون التمور فيها طرية القوام عدا الصنف رحماني الذي كان قوام التمر فيه شبه جاف. واستخدمت الدراسة برنامج التحليل الالي لتحليل المكونات الرئيسة للثمار في مرحلة الخلال, فتبين ان الاصناف انقسمت بمجموعتين وحسب التقارب في البيانات المغذاة للبرنامج فانعزل الصنف الزريجي بمجموعة مستقلة لكون بياناته متفردة, اما المجموعة الثانية فقد توزعت الاصناف فيها الى تحت مجموعة, صغرى ضمت الاصناف دكلة داود ونجمة واقترب منهما الصنف وردية, اما الاصناف الباقية فتوزعت هي الاخرى بتحت مجموعتين. استخدمت الدراسة الجزيئية تقانة PCR RAPD لتثبيت البصمة الوراثية للاصناف المدروسة ولكشف الارتباط الوراثي بينها, وجرى باختبار عشرة بادئات عشوائية من مجموعة OPA وبادئان من مجموعة OPB. تم تحليل نواتج التفاعلات للبادئات مع Genomic DNA الاصناف كافة وكل على حده لتحديد مدى كفات البادئ فكانت النتيجة ان اربعة من البادئات ( OPA0.2 وOPA0.3 وOPA0.5 وOPA0.6) نجحت في كشف التباين بين الاصناف المدروسة, وبينت نتائج التحليل العنقودي للنواتج التفاعلات للبادئات ان الاصناف توزعت بمجموعتين, صغرى ضمت كل من الاصناف زريجي ونمروشة والصنف ام اللسين ومجموعة كبرى ضمت الاصناف الباقية, وقد توزعت فيها الاصناف الى تحت مجموعة ثانوية. مما يشير الى الاصول المشتركة, وبينت الدراسة امكانية انتخاب اصناف بذرية تمتاز بخصائص ثمرية جيدة وتكثيرها لدعم الاقتصاد الوطني. | This study was performed during agronomic season in 2015 on 11 cultivars (ghaibany) Phoenix dactylifera L. that grow in basrah, and these cultivars are new recorder. Twenty eight characteristics of vegetative growth were studied, seed characteristics were studied also. It was concluded that the studied cultivars varied in there vegetative and fruit qualities. The study showed that both cv Nabawy and cv Najma distinguished by semi - opened crown but the other cultivars had closed crown . In addition, cv Najma marked by waxen green leaves. The rate of leaf length ranged from 2.68 m in. cv Wardiah to 4.35m in cv Nammrosha. besides the length of spine area ranged from 56 cm in cv Deglat Abd cultivars to 160 cm in cv Am - Ellsian. The cultivars also varied in the rate of spine number which was 18 in cv Wardiah an 47 in cv Am - Ellsian. The cultivars differed in the style of spine arrangement on rachis since cv Bermany distinguished by the dual style of spine arrangement, while in cv Wardiah marked by single dual style, however the single style was prevalent in other cultivars. The study clarified that the percentage between spine area to the total length of the leaf was one of qualities that characterized by stability that differed from other vegetative characteristics. As for fruit characteristics, the length of peduncle of cultivars concerned ranged from 66 in cv Rahmany cultivars to 152 in Bermany and that was regarded long peduncle. cv Deglat Dawood marked by colour of peduncle which was yellow look like rosy, whereas cv Bermany and cv Najma the colour of their peduncle were yellow look like green and yellow in the rest. As for physical characteristics of studied fruit, cv Deglat Dawood recorded the lowest rates in the volume of fruit during the phases of khalal and dates, the volume was 5 c.m and 2.6 c.m respectively, cv Zraigai distinguished by the bigness of the volume of the fruit for both phases of khalal and date 17c.m and 10.24 c.m. The taste of khalal in the cultivars of cv Deglat Abd, cv Wardiah, Rahmany, cv Zreigai was tannic while the taste of other cultivars were sweet. cv Najma cultivars distinguished by being so early in maturity, the other cultivars were early in maturity but cv Bermany was middle in maturity. The dates of studied cultivars were soft except cv Rahmany that was semi - dry. The study used a program of automatic analysis to analyze the main components of fruit in khalal phases the, it became clear that the cultivars divided into two groups according to the convergence data that fed to the program. cv Zraigai cultivars was isolated in independent group because its data were unique, but the second group ,cultivars in which were classified in small group included cultivars of cv Deglat Dawood and cv Najma and cv Wardiah approached to this group. The other cultivars classified into two groups.The molecular study used P C R RAPD technique to fix genetic print of studied cultivars and to discover the genetic link among them. Ten of random primers of OPA group were tested and two of OPB were tested too. The products of reaction of primers were analyzed Genomic DNA for cultivars totally and individually; to determine the efficiency of primer the results was that for of primers succeeded in discovering the variation among cultivars concerned. The result of cluster analysis of reaction products of primers showed that the cultivars divided into two groups : small group included cv Zairgai, cv Nammrosha and cv Am - Ellsian cultivars, and big group contained the other cultivars in which cultivars distributed into secondary group and that refer to the common origin. The study showed the ability of electing and increasing Ghaibany cultivars that marked by good characteristics to support national economy

دراسة تصنيفية وحياتية للفطريات الناقصة الملونة في البصرة == Taxonomical and Biological study of Dematiaceous imperfect - fungi in Basrah

Author name: زينب خلف عبد الله
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: During this study 23 species belong to 10 genera of imperfect fungi were isolated from different natural sources ( plants, soil , and ari) from Basrah govermanete using different isolation methods.Among the isolated fungi the genus Alternaria was selected to identify its species by using the morphological characteristis by light microscopy and Scaning Electron in Microscope (SEM) and verified by molecular and chemical methods. Eight species of Alternaria were identified namely; A.alternata (isolate1 and 2) A.chlamydospora A.citri, A.raphani , A.longipes , A.infectoria A.tenuissima and A.chrysanthemi based on light microscope examination.Taxonomic relegation of these species were confirmed by SEM image. SEM revealed that there was differences in the conidial wall ornamentation .The molecular analysis using PCR depending on the loci ITS1, and ITS4 700bp which confirmed the morphological identification of most of species namely A.alternata (isolate 1 and 2) , A.chlamydospora , A.longipes , A.infectroia and A.tenuissima. However , A.citri was related to A.alternata, A.raphani was belonged to A.infectoria and A.chrysanthemi is belonging to A.lolii. Nevertheless ,by using the loci OPA1 - 3 there was no variation among the species of Alternaria ,this is may be due to the inadequate method applied.The genetic variation of 36 isolates of A.alternata was studied using 9 - primers by RAPD - PCR technique. All primes gave an amplification of DNA for all the fungal isolates. Among the primers OPC08 exhibited a highest efficacy reaching 235 total bands .In the present study the antibacterial activity of fungal crude extract was tested. It appeared that the extract exhibit an antibacterial bioactivity against E.coli , S.aureus , S.epidermidis and S.saprophyticus.However, the extract of A.alternata (isolate 1 and 2) showed an inhibition growth against all the tested bacterial strains. This study was also revealed that the fungal filtrates of Alternaria species possessed various chemical compounds. However, there were differences in the chemical composition among the fungal filtrates.The results showed that Alternaria species ( A.alternata isolate 1 and 2, A.chlamydospora A.citri, A.raphani , A.longipes) were capable to biosynthesize silver nanoparticles, when the fungal filtrate were treated with AgNO3 solution after72 hrs incubation .Silver nanoparticles synthesis was checked by the change of colour from yellow to brown and confirmed by Uv - vis spectrophotometry at wave length (420, 440 nm). SEM image showed that the silver nanoparticles with spherical shape and size ranged between (20 - 72nm). Aconclusion can be derived that application of SEM,chemical analysis of fungi extracts ,molecular analysis and the silver nanoparticles characteristics are useful in the taxonomy of fungi to solve the overlapping of the morphological identification .Taxonomic key were prposed for the species of the genes Alternaria based on the morphology , chemical compound with the fungal extracts and size of silver nanoparticles synthesized by fungi .Melanin pigment identify by TEM microscope and it appeare like dark drop inside the cell .

دراسة المايكوبلازما المصاحبة لحالات العقم في الرجال في محافظة البصرة == Study of Mycoplasma associated with men infertility in Basrah province

Author name: زهراء كاتب جمعة
Supervisor name: غيداء جاسم عبد النبي الغزاوي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study aimed current was to determine the role of Mycoplasma in men infertility in Basrah province . A100 seminal fluid specimens were collected from men who were admitted to infertility center in Basrah province from 1/10/2015 to 31/5/2016 .Besides 50 semen specimens were collected from those who determine fertility men as control group. Samples then were cultured on Monophasic - Diphasic - Culture - Setup(MDCS) to isolation Mycoplasma then the samples were cultured on Blood agar, MacConkey agar and Chocolate agar to isolation bacteria other than Mycoplasma .The isolated Mycoplasma from 48 cases were concluded five species : Ureaplasma urealyticum 38% ; Mycoplasma hominis 21%;Mycoplasma genitalium 15% ; Mycoplasma pirum 15% and Mycoplasma fermentans 11% . Where the last two species are first recorded in Iraq from semen.This Study also recorded that the patients of 30 - 39 years old were of high infertility rate and Mycoplasma infection percentage.High the percentage of a single infection was related to U. urealyticum and Mycoplasma hominis .By using the double Vitek 2 system 22 other bacterial species were diagnosed from seminal fluid. some of these species were first recorded in Iraq and represented types next : Staphylococcus haemolyticus ,,Enterococcus faecalis Staphylococcus hominis , Staphylococcus warneri ,Kocuria rosea , Streptococcus pneumoniae , Kocuria kristinae ,Granulicatella elegans , Vibrio cholera , Aeromonas salmonicida ,Enterococcus faecium , Morganella morganii, Enterobacter cloacae ,Alcaligenes faecalis , Staphylococcus xylosus , Micrococcus lutes ,Granulicatella adiacens, Kocuria varians,And the most exhibited and isolated bacterial species was of Staphylococcus haemolyticus.Mycoplasma infection were noticed in patients who were suffering from other diseases like varicose 18.75% and diabetes 8.33% and bladder nerve pressure 4.16% .In this study the primary infertility was 82% included 39 cases infected with Mycoplasma , while for the secondary infertility 18% nine cases were infected with Mycoplasma .High infection rate of Mycoplasma 69.23% was recorded in patients who underwent a varicose other Surgery compare with the other patients.Mycoplasma infection for the five species was clear quantity and quality in semen analysis for infertile men was less than control group .Where the semen volume was very small and the sperms count was effect on also the sperm morphology and activity (mobility) were affected by Mycoplasma infection . Pus cells and RBC. Were appeared in semen.This study also , confirmed that there is a 7% of semen from infertile men , was free from bacterial infection.

بعض الجوانب الحياتية للروبيان الدخيل والروبيان Macrobrachium nipponense (De Haan,1849) من Metapenaeus affinis (H. Milne Edwards, 1837) بعض المواقع المائية في محافظة البصرة == Some Biological aspects of the Invasive Shrimp Macrobrachium nipponense (De Haan,1849) and The Shrimp Metapenaeus affinis (H. Milne Edwards, 1837) from some Aquatic sites of the Basrah province

Author name: انوار مالك جبار المالكي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study intended to shed the light on some biological aspects of the invasive oriental river Shrimp Macrobrachium nipponense compared with that of Jinga shrimp Metapenaeus affinis . For this purpose, Monthly samples were collected and chosen from four Aquatic sites was chosen in the Basrah province (one at each of Al - Mashab and Al - Sallal Marshes and two at Al - Garmah River) during the period from July 2015 to June 2016. collection was done by Fishnet pulled by a boat .The Shrimp numbers were calculated during the time unit (ind./hour) .The Water’s temperature and salinity were studied, it ranged from 15 - 33 ͦ c and 2 - 9.1psu respectively. From a total of 3326, individuals were collected during the study period, only 7% were belong to the first shrimp (M. nipponense ) Compared with 93% to the second shrimp( M. affinis ).The number of each species studied shrimps, varied the monthly. The high number of M. nipponense was 38(ind./hour) recorded during July 2015 and June 2016, while the lowest was 2 (ind./hour) recorded during October and November 2015 . For M. affinis The number of individuals ranged between 980(ind./hour) during August 2015 and 29 (ind./hour) during November 2015 .The comparative monthly percentages were high during seven and six months in the marshes sites recorded for first and second shrimp respectively. The monthly variation in percentages of the two species of shrimps was studied, It ranged (for M. affinis) from 98.8% during August 2015 to 56.8% during July 2015 , while for M. nipponense , It ranged from 43.2% during July 2015 to 1.2% during August 2015 .The results of Frequency showed that the highest percent (100%) was recorded in four months for M. nipponense , these are July, October 2015 February and May 2016. While M. affinis was recorded in most months.Summary bThe population sizes for the two species were studied. For the first species( M. nipponense ), the size class 50 - 60mm dominant during all months of the study period, While for the second species (M. affinis), the size classes 40 - 50, 50 - 60, 60 - 70, and 70 - 80 were recorded during all the months for the first shrimp, the highest percent was 47.3% recorded for the size class 70 - 80 mm , While there is no individuals recorded for the size class 10 - 20 mm during all the months except July 2015 for the second shrimps ,the percentage ranged between 41.4% and less than 1% recorded during May 2016 to March 2016 respectively. On the other hand , the total Frequency for M. nipponense ranged from 83.3% to 0% for the size classes 70 - 80 mm and 10 - 20 mm respectively, While for M. affinis was from 100% recorded for the classes 40 - 50, 50 - 60, 60 - 70 and 70 - 80mm to 8.3% for the class .The Length - Length and Length - weight relationships for the two species were studied The results showed positive correlation between the total length and each of cephalothorax length and abdomen length (r= 0.932 and r= 0.945 respectively, P≤0.01 ) for M. nipponense , Also M. affinis , the correlation was positive for the total length and each of cephalothorax length(r= 0.938) and abdomen length (r= 0.971) (P≤0.01 ) . The correlation was positive between the total length and wet weight for each M. nipponense (r= 0.918 P≤0.01) and M. affinis (r= 0.913 P≤0.01).The Statistical analysis for the weight - weight relationships showed positive correlation between dry and wet weight for M. nipponense (r= 0.966, P≤0.01) and M. affinis (r= 0.918 P≤0.01).The correlation between the lengths of the body and the 2nd chela for male & female of M. nipponense (r= 0.909 and r= 0.823 respectively), these values of correlation refer to the continuous growth of the 2nd chela in male even after the adult stage .Summary cThe concentrations of ten heavy and light metals in shells & muscles of the two shrimps were studied. The results showed a significant differences in eight of them (Cadmium, Potassium, Calcium, Iron, Magnesium, Nickel, Zinc and Sodium) . While there is no significant differences recorded for Lead and Cobalt .The study of some biological aspect which concerned with distribution of eggs on pleopods of females of M. nipponense , showed that the highest mean of eggs was 547.3 (38%) carried on the 2nd pleopod while there are fewer means carried on 1st and 3rd pleopods , and no carry eggs (0%)were carried on the 5th pleopod. The Statistical analysis showed that there was significant differences between the eggs carried on 4th and 5th pleopods and each of the rest ones . lengths of pleopod was positively correlated with the number of eggs (r= 0.661, P≤0.01) The weight of ovigerous female was positively correlated with weight of eggs (r= 0.835, P≤0.01) .The combined effects of salinity and temperature on survival of newly hatched larvae (zoea1 and zoea2) of M. nipponense for 24 & 48 hours’ were tested.The highest survival percentage (100%) for zoea1 was recorded in 12 psu salinity in each of 18, 22 and 26 ͦ c ,While the lowest survival (0%) was recorded in distal water in each 30 and 34 ͦ c . the highest survival percentage was recorded in 12 psu salinity in each 18, 22, 26 and 30 ͦ c. While the lowest one recorded in distal water and 34 ͦ c. The Statistical analysis showed that the percentage survival correlates negatively with temperature and positively with salinity each alone. the combined effect of salinity and temperature on survival was positive with percentage ranged between 38 - 56 % .

عزل وتشخيص بعض الانواع الجرثومية من مياه الانهر الداخلية في مدينة البصرة ودراسة قابليتها على المعالجة الحيوية للعناصر الثقيلة == Isolation and identification bacterial species from inner rivers in Basra governorate and study their ability to Bioremediation of heavy metals

Author name: انوار عبد الوهاب مكي
Supervisor name: علي عبود شريف
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted during November and December 2013.It included studying six inner rivers branching from Shatt al - Arab river in Basrah governorate.These rivers are Abu Al - khasseb, Al - Ribat, Al - Khandaq, Al - Ashar, Al - Khura and Al - Sarraji .The study included isolation and identification of three species of genus Bacillus which are B. licheniformis,B. megaterium and B. badius ,and two species of genus Micrococcus which are M. halobius and M. kristinae .Physical and chemical characteristics of the water samples were measured, which including pH,temperature,electric conductivity and Dissolved oxygen. The results showed that there were not correlation between physical and chemical characteristics and the concentrations of the heavy metals except for water temperature which had a negative significant correlation with the concentrations of nickel and lead.The concentrations of heavy metal dissolved in water (copper, nickel , lead and cadmium ) were measured. The concentrations of Cu+2 and Pb+2 were higher than that of Ni+2 and Cd+2 in the water .However,in general the concentrations of Cu+2, Ni+2, Pb+2 and Cd+2 were high in comparison with the local and international standards.All species of bacteria showed high resistance to the heavy metals ions starting from concentration of 1 mg/l. B. licheniformis was recorded high resistance to Pb+2, Cu+2 and Ni+2 which reached 2600 mg/l, 500mg/l and 300mg/l respectively. M. halobius was also recorded a high resistance to Pb+2 and Cd+2 with 2600mg/l and 400mg/l respectively. The highest resistance was recorded by M. kristinae to nickel with 300 mg/l . The mid resistance was by B.badius to all studied heavy metals and the lowest was by B. megaterium to all heavy metals studied in comparison with the other species of bacteria.The identified bacteria were used in the process of bioremediation of the heavy metals (Cu+2, Ni+2, Pb+2, Cd+2) with different concentrations (25, 50 and100 ) mg / l. B. licheniformis showed high ability to remove Ni+2, Cu+2 and Cd+2 than other species of bacteria, which reached (28.30%, 44.34% and 24.27%) mg/l. . respectively. B. badius showed a high capability for removing Cu+2 with 45.78% mg / l .Where as B. megaterium showed a high capability for removing Pb+2 with 23.43% mg / l.. M. halobius had the highest capability for removing Cd+2 with 25.42% mg / l. and M. kristinae had a mid capability to remove the heavy metals under studied.The study included observing the effect of the incubation period on removing the heavy metals ions.The results found that the best period was 72 hours in comparison with 24 and 48 hours.Also, the study included the effect of initial concentration in the process bioremediation.The results showed that the concentration of 25mg/l was the best for removing the heavy metals ions among the other concentrations, 50 and 100 mg/l.

عزل وتشخيص احد قلويدات الايض الثانوي لبعض الطحالب ودراسة فعاليته الحيوية == Isolation and identification one alkaloid of secondary metabolites from some algae and study of biological activity

Author name: انفال نوري عباس اللفتة
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study includes an isolation, Identification and purification of three species of algae, two of them belonging to cyanobacteria they are Oscillatoria brevis and Nostoc carneum . The third one was Enteromorpha intestinalis which belong to green algae which was from different location in Basrah .There are two extract were prepared from the algal species Alcohol , and alkaloid extracts , Alcoholic extract prepared to know what was compound it had and alkaloid extracts to test bioactivity of algae Cytotoxcity also was carried out on human red blood cells, the results revealed that alkaloid extract from E.intestinalis was nontoxic, whereas bioactive compounds isolated from O.brevis , N.carneum. Showed hemolytic action .The bioactivity of alkaloid extract was examined to elucidate their on ability to inhibit the growth of gram Positive and negative bacteria. .Biological activity of alkaloid extracts of three algae isolated were determined by using the Minimal Inhibitory Concentration (MIC) againsttwo bacterial isolates. And also test the effect of filtrate pure algal culture on growth of plant pathogenic fungi ,also apparently that the algal alkaloid extract and isolated compounds exhibits antioxidant.The antitumor activity of the algal alkaloid extract from E.intestinalis against Rhabdo myosarcoma cell line was examined. The results showed that crude alkaloid extract possessed an antitumor bioactivity at low concentration 0.78mg / ml .In this study also was carried out to investigate the possibility of preventing the hypercholesterolemia by using alkaloid extract of E.intestinalis .it also aimed to study the effect of use this extract in reducing the Plasma Total Cholesterol (TC) , Triglyceride (TG), Low - Density Lipoprotein (LDL) , Very Low - Density Lipoprotein (VLDL) and no effect on High - Density Lipoprotein (HDL).The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR) and Gas Chromatography / Mass spectrum (GC - MS). The results of such analyses showed that alkaloid extract from E.intestinalisa has three compound these are Hexadecanamide , Methenamine and Benzenedicarboxylic acid, diisooctyl est and alkaloid extract from N.carneum showed that has three compound 1,2 - Benzenedicarboxylic acid diisooctyles,ا9 - Octadecenamide, (Z) - )ااااا and Didecyl phthalate .Hexadecanamide purification from alkaloid extract of E.intestinalis depended on physical and chemical properties The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR); Gas Chromatography / Mass spectrum (GC - MS) and this component showed bioactivity on gram. Positive and negative bacteria and it has also antioxidant activity

استخدام عوامل استحثاث لانتاج مؤيضات ثانوية من بعض عزلات انواع الفطر واختبار فعاليتها الضد بكتيرية والخلايا السرطانية Penicillium والكولسترول == Use of inducing agents in secondary metabolites production from some Penicillium isolates and assay their activity against bacteria , cancer cells and cholesterol

Author name: امل صالح عبد الرضا
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was designed to investigate active compounds from different Penicillium isolates ,that isolated from different sources , using different media . Eleven isolates related to five species : P. chrysogenum, P.paxilli , P. citrinum, P. cosmopolitanum, P. digitatum were isolated. After primary screening was performed , the best isolates that have high antibacterial activity against gram positive and gram negative bacterial was selected. The selected isolates were cultured on fermentation media according to the suitable media for each isolate, and the active compounds were extracted using ethyl acetate as a solvent. P. cosmopolitanum and P. chrysogenum were cultured on penicillin production medium (PPM) , P. paxilli was cultured on Aspergillus complete medium (ACM) and P. digitatum and P. citrinum were cultured on solid state fermentation media consist of wheat bran . The efficiency of fungal crude extracts from each isolate against the reference strains S. aureus NCTC 6571 and E. coli ATCC 25922 and against Candida albicans (pathogenic and reference strain)was examined, and the Minimum inhibitory concentration (MIC) was determinated for each extracts.The inhibition zone of fungal extracts against E. coli and S. aureus ranged between (20 - 50) mm , and they had high activity against C. albicans ( reference and pathogenic isolate) ranged between 25 - 45 mm.Induction of secondary metabolite production by the selected fungi was carried out using biological factor (Streptomyces sp.) , chemical agent (colchicine) and physical agent by UV radiation. Apparently that the biological induction by Streptomyces isolate was the best compared with the other treatments.The results revealed that the two fungal extracts ALO1 and Apax exhibit a high antioxidant activity at low concentrations and volumes of the extract compared to ascorbic acid as control. ALO1 had radical scavenging activity (RSA%) reach 99.9% at volume 3μl , and Apax had RSA 91.6% at concentration 500μg/ml. Also, the fungal extract ,ALO1, showed a significant activity as anticancer agent against RD - cell line. The IC50 of ALO1 was 8.5μl after 24h and 23.76μl after 72h. The hypercholesterolemia ability of ALO1 in vivo and in vitro was assayed . In vivo experiment designed using female albino mice and divided into three groups each one contain 8 mice : group 1 treated with 10μl , group 2 treated with 20μl of ALO1 , and group 3 treated with normal saline as control group. The results showed that total cholesterol , triglycerides, LDL and VLDL were significantly reduced when compared with control group while the HDL level was increased in comparing with control group. The chemical analysis of the crude extracts showed that Apen2 contained 259 μg/g of penicillin G when analyzed with HPLC apparatus . Also the GC mass analysis of ALO1 showed that Docasadionic acid initialize area76.18% and Palmitic acid initialize area 15.09% from the total identified compounds.Whereas the GC mass analysis of Apax showed that Hexandioic acid initialize area40% and Stearolic acid initialize area 35.57% from the total identified compounds.In addition , it was detected the presence of penicillin biosynthetic gene cluster : pcbAB, pcbC and penDE in P. cosmopolitanum and P. chrysogenum.The result showed that gene pcbC and penDE were found in the genomic DNA but the gene pcbAB was not amplified by PCR.
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