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تحضير حليب فول الصويا المتخمر ودراسة صفاته الكيميائية والميكروبية والحسية وتاثيره على بعض المعايير الذموية للجرران == Preparing Fermented Soymilk and Studying the Chemical, Microbiological and Sensory Characteristics and its Effects on Certain Blood Criteria of Rats

Author name: علاء سعد ناجي الشريفي
Supervisor name: علي احمد ساهي | علاء كرين نعيمة
General topic: Agricultural sciences
Specific topic: Food
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study includes soymilk extraction from its grains with three different extraction methods and study the chemical composition, physical properties of the milk for each method. the second method was chosen because its produce milk includes 10.44% total solid material, the chemical composition consist of moisture 89.56%, protein 4.188%, fat 2.47%, ash 059%, carbohydrate 3.192%, and phytic acid 0.225% while the rate of mineral ions ferric, magnesium, phosphor, calcium and potassium (0.004, 0.1, 0.03, 0.09 and 0.063) respectively. The starter company which consist of Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium sp. were used in soymilk fermentation and the best incubation temperature 40 °C for 7 hours , inoculation volume 5% the log of the total count of lactic acid bacteria 10.74 cfu / gm and the log of Streptococcus thermophilus 8.56 cfu / gm and the log of L. acidophilus 7.86 cfu / gm, the log of Bifidobacterium 7.65 cfu / gm, the pH was 4.42 and the rate of total acid was 0.8%. The cold storage effect for 28 days were studied for probiotic bacteria survival, the log of total bacterial account 9.78 cfu / gm, the log of St.thermophilus 8.07 cfu / gm and the log of Bifidobacterium sp 6.5 cfu / gm, while the log of L. acidophilus account was the most effect 4.1 cfu / gm the pH value decreased for 4.6 while the total acidity percentage was 0.81%.The chemical composition of yoghurt was studied The percentage of each moisture, protein, fat, ash and Carbohydrate were (88.8, 4.2, 2.3, 0.82 and 4.6) respectively, the phytic acid rate decreased for 0.075% The experimental animals were fed with milk soybean yogurt with 3 ml and 2 ml for 40 days , the result showed decreasing the rate of cholesterol and triglyceride in T1 treatment which were( 114.24 , 74.5 mg / deciliter) respectively compared with control (97.78, 124.67 mg / deciliter) the hematocrit, hemoglobin, White blood cells, thrombocytes, red blood cells, and the average quantity of hemoglobin, the size of blood cells and the hemoglobin concentration in T1 treatment were (49.66,14.76, 18.96, 246.3, 6.4, 16.4, 62.33, 30.53) respectively compared with control (42.0, 12.0, 12.6, 206.3, 6.0, 13.53, 45.33, 24.6) respectively. The standard of immunity were investigated for the experimental animals the rate of neutrophil degreased in T1 treatment which was 7.83% while in control 14.72% the rate of lymphocyte increased in T1 treatment 90.62% while degreased in control 82.0%.The sensory evaluation showed that the yogurt produced from soybean milk include garlic taste had the highest degree 86.6% while the yogurt without addition of taste had the lowest value which was 53.7%.

تاثير موعد الزراعة ومسافتها والرش بالهيومس السائل في النمو وحاصل البذور والزيت لنبات الخلة البلدي Ammi visnaga (L.) Lam. وفعاليته التثبيطية لبعض الاحياء الدقيقة == Effect of Sowing Date, Plant Spacing and Spraing with Liquid Humes on Growth and Yield of Khellah Plant Ammi visnaga (L.) Lam. and its Inhibition Activitie on Some Microorganisms

Author name: علا عدنان فائز حبيب الجابري
Supervisor name: عصام حسين علي الدوغجي
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted during the growing season of 2013/2014 in the Medical and Aromatic Plants Unit Field, Agricultural Collage, Basrah University, to study the effect of sowing date, plant spacing and spraying with liquid hummus and their interaction on growth and seed and fixed yield of Khella plant Ammi visnaga (L.) Lam. " Syrian Local " and its biochemical effects.The study Included 12 factorial treatments, which were the combinations of two sowing dates 1/10 or 20/10, three plant spacing 20, 30 or 40 cm and spraying with liquid humus in concentration of 0.75 ml. l. - 1 or without ( spraying with distill water) and their interactions. A Complete Randomized Block Design in a factorial experiment was used with three replicates. The results analyses with SPSS program and Least Significant Differences Test (L.S.D) in level 0.05 was used to compared The most important results may be summarized as follows : - 1. Effect of sowing dates : Plants sown on 1/10 gave a significantly increases in plant height, leaf number. Plant - 1, number of main lateral branches. plant - 1 , earliest in inflorescences appearance, number of inflorescences. plant - 1, total seed yield. plant - 1 (54.3 gm), weight of 1000 seed, seeds productivity. hectare - 1 (4754kg), total fixed oil. plant - 1 (2.9gm), fixed oil productivity. hectare - 1 (309.5 kg), leaf total soluble carbohydrates content, seed total soluble carbohydrates content. Whereas plants grown on 20/10 had a significantincreases in dry matter percentage, fixed oil percentage ( 8.3%) and seed total protein percentage.2. Effect of plant spacing : Plants cultivated at 40 cm a part had a significant increases in leaf number. plant - 1, earliest in inflorescences appearance and number of inflorescences. plant - 1. Whereas plants cultivated at 30 cm a part had a significant increases in weight of 1000 seed and leaf total soluble carbohydrates content, Plants cultivated on 20 cm a part gave a significant increases in seeds and fixed oil productivity. hectare - 1 (3543kg and 311.2 kg), respectively.3. Effect of spraying with liquid humus : - Plants sprayed with liquid humus had a significant increases in leaf number. Plant - 1, dry matter percentage, number of inflorescences. plant - 1, total seed yield. plant - 1 (34.0 gm), seeds productivity. hectare - 1 (3543kg), fixed oil percentage (8.1%), total fixed oil. plant - 1 (2.6gm), fixed oil productivity. hectare - 1 (267.2 kg) and leaf total soluble carbohydrates content.4. Effect of the interactionsThe Interactions between the study factors, showed a significant effect in all studded parameters except leaf total chlorophyll content.5. The activity of the Khella seed and leaf extracts against certain types of pathogenic bacteria to human.The activities of aqueous, methanolic and ethanolic extracts of either leaves or seed were tested against some types of bacteria Escherichia coli, Pseudomonas aerugnesa and Staphylococcus aureus at fiveconcentrations 50, 100, 200, 300 or 400 mg. L. - 1. The aqueous extract of the seeds showed inhibition effect in all studied concentrations, the effect increased with the concentration increases.6. The activity of the Khella seed and leaf extracts against two pathogenic fungi Aspergillus nigerCandida albicanThe activities of aqueous, methanolic and ethanolic extracts of either leaves or seed were tested against two fungi Aspergillus nigerCandida albican at five concentrations 50, 100, 200, 300 or 400 mg. L. - 1. All the extracts showed inhibition effect in growth diameters of both fungi, the methanolic extract of leaves was the more effected

مسح وتشخيص بعض الملقحات الحشرية ومصادر حبوب اللقاح في محافظة ميسان == Survey and Identification Some Pollinators and Pollen Resources in Missan Province

Author name: عقيل عبد الرزاق كريدي
Supervisor name: طه ياسين مهودر العيداني | خالد عبد الرزاق فهيد
General topic: Agricultural sciences
Specific topic: Plant - Prevention
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: survey and identification of the most important pollinators were conducted in three locations in Missan province (Amarah, Maymonnh , Almajar alkabir) , for the purpose to survey and identify the most important pollinators in the province,In view of the diversity of plants and insects pollinators the study select three plants , ber tree Ziziphus spina - christi and two crops are alfalfa Medicago sativa and Egyptian clover Trifolium alexenderianum to confine the pollinating insects. Honey bee Apis mellifera be one of the most important pollinator . So pollen sources in the sediment of honey and propolis is studied to identify botanical origin that visited by insects to keep them and provide fingerprint about plant diversity in the province.The study showed the following : 1. there are many insects visiting flowers of ber , alfalfa and clover were some of them are classified to the species level, while others were classified to the generic level such as wild bees Andrena sp. , Megachle sp., Tetralonia sp.And some species belong to family syrphidae such as Eristalis sp. , Syrtta sp. and Syrphus sp..2. Honey bee Apis mellifera is the most common and attracting to the two experiment crops flowers . where on average of 0.978 insects per 3m² during five minutes in alfalfa and 1.2 insects in clover were recorded. the second important is the wild bee Andrena sp. with an average of 0.662 insects in alfalfa and 0.8 in clover. 3. Honey bee were holding maximum quantity of pollen from alfalfa and clover . The average load was 67874 Pollen Grains (PG) was recorded . This quantity may be greater when it's estimated in the bee when they returns to hive .Wild bee Andrena sp. loud of pollen grain counted 54233 PG and ,IIthe wild bee long horn Tetralonia sp. carry about 51664 PG , while the leaf cutter wild bee Megachile sp. and white - banded digger Amegilla quadrifasciata carry 44256 and 38154 PG respectively , The least loud was carried by syrphid fly Eristalis aeneus was 27657 PG in it's gut .4. Honey bee Apis mellifera was the most insect spending time of foraging pollen on clover in average 9.76 second and 8.85 second on clover and alfalfa flowers respectively in the daylight , the next were the miner bee Andrena sp. spending 5.53 sec and 4.32 sec on clover flowers and alfalfa respectively . The white - banded digger wild bee A. quadrifasciata and the leaf cutter wild bee Megachile sp. have not seen visiting clover while they visit alfalfa flowers .5. pollen spectrum in spring and autumn honey sediment was studied , wich showed an important and new sources the tamarisk Tamarix brachystachys that is record the first time as a predominant pollen source , this source rated over than 45% in three sample of spring honey while as known between researchers and bee keepers that spring honey called a Eucalyptus or clover honey. Some weed were also important as pollen sourcessuch as Capparis spinosa , Sonchus oleraceus in addition to Prosopis juliflora and some species of the Families Cruciferae , Compositae and Umbelliferae.6. the light microscope showed that The most important resource of propolis Eucalyptus camaldulensis , Tamarix spp. and Prosopis juliflora

تاثير اضافة الخليط التازري لمسحوق حليب الكيفير المجفف والمصنع في بعض الصفات الانتاجية والفسلجية والمناعية لفروج اللحم == Effect of Addition of Synbiotic of Dried and Processed Kefir Milk Powder in Some of The Productive, Physiological and Immunological Characteristics of Broiler Chicken

Author name: عقيل طوينة عودة
Supervisor name: عبد الله عبد المنعم محمد
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Four experiments were carried out for this study, two field and two laboratory, to manufacture a Synbiotic for milk powder Kefir ( MPK ) and loaded with some feed material with or without sugar beet milled or Helianthus tuberosus powder and the effect of adding it to some of the production , physiological and immunological characteristics of the chickens . Where the first laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agricultur - University of Basrah , for the period from 10 / 4 / 2015 to 7 / 5 / 2015 for the purpose of manufacturing a Synbiotic of MPK.The second field experiment was conducted in a commercial field for poultry in Al - Zubair / Basrah during the period from 24 / 1 / 2016 to 27 / 2 / 2016 , which included selecting the best biomass of MPK loaded on wheat , corn , soybeans and wheat bran with the addition of Helianthus tuberosus powder , So that one gram of MPK and loaded on the previous materials can bacteria . In this L. acidophiluscfu / ml 8least 10be provided at experiment, 450 unsexed one day of the Ross 308 chicks, at a rate of 40 g / chick were distributed randomly to six Treatments , three replicates per treatment , 25 birds per replicate and the treatments were as follows ; T1 : Control treatment included a standard diet . T2 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach . T3 : A standard meal with 3 g of MPK loaded on wheat/ kg feed .T4 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T5 : A standard meal with 3 g of MPK loaded on soybeans / kg feed. T6 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed .The third field experiment was conducted to determine the best effect of the synbiotic MPK loaded with wheat, corn, soybeans and wheat bran and compared with three foreign probiotics in the productive, physiological and immunological characteristics of chicks. This experiment was conductedbduring the period from 4 / 10 / 2016 until 9 / 11 / 2016, using 600 unsexed chickens with one day of the Ross 308 at a rate of weight of 40 g / chick .The chicks were randomly distributed into eight treatments with three replicates per treatment and 25 birds per replicate. The experiment was designed as follows : T1 : Control treatment included a standard diet, T2 : A standard meal with 3 g of MPK loaded on wheat/ kg feed . T3 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T4 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed. T5 : added treatment 1/2 g of probiotic Labzyme / kg feed , Korean - made , T6 : added treatment 1/2 g probiotic Biozyme / kg feed , German - made , T7 : added treatment 1/2 g probiotic Biolac / kg feed , Vietnamese made , T8 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach .The fourth laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agriculture, University of Basrah for the period from 6 / 3 / 2016 to 7 / 4 / 2016 for the study of antibacterial activity in the kefir milk and compare it with Biozyme , Pencilin, Gentamycin, Tetracyclin and Neomycin against Staphylococcus aureus and Escherichia coli causing bacterial necrosis with Bacterial Chondronecrosis with Osteomyelitis ( BCO ) . The results of the study included the following : First laboratory experiment ;The results of the first experiment showed that the wet biomass rate of fermented kefir milk was 70 % . This high percentage is evidence of the growth of Lactate Bacteria in this product. The results showed that wheat bran and wheat bran and corn were the best loaded significant materials (p <0.05) Compared to soybeans in terms of growth and reproduction of lactic acid bacteria. Adding Helianthus tuberosus powder increased the number of bacteria in dried kefir milk compared sugar beet milled , which reduced the number of bacteria in MPK .Second field experiment : cThe results of the experiment showed the following : 1 - There were significant increase (p <0.05)) in the final body weight rate and the body weight gain and Food conversion efficiency, as well as a significant decrease (p <0.05) In the amount of feed consumed for the sixth, third and fourth treatments, supplemented with synbiotic MPK loaded on wheat bran, wheat and corn compared with other treatments .2 - Treatments sixth , third , fourth had significant (p<0.05) decrease mortality percent as comparing with control and treatment two .3 - The sixth , third , and fourth treatments were significantly higher (P <0.05) in the production index and the performance index and drcrease value of economic efficiency compared with other treatments .4 - Total protein and globulin in plasma serum were increased significant (p<0.05) and decreased (p <0.05) in the concentration of triglycerides and cholesterol in the sixth, third and fourth treatments supplemented with synbiotic MPK , loaded on wheat bran , wheat and corn , as well as the increased of the third, sixth and fourth treatments significantly (p <0.05) in the concentration of ALP enzyme compared to other treatments .5 - The results showed that the fifth, sixth, third, and fourth treatments did not differ significantly in antibodies titer against Newcastle ( NDV) and the Comboro ( IBDV ) disease, but significantly exceeded (p <0.05) compared to the first and second treatments .Third field experience : According to the results of the second field experiment, the best three treatments were added to the synbiotic MPK and loaded on wheat bran , wheat and corn . It was compared with three foreign prpbiotics , the treatment of addition of amoxicillin antibiotic and control treatment. The results of the experiment showed the following ;d1 - The second , fourth , third and sixth treatments, supplemented with probiotic, MPK , loaded with wheat , wheat bran , corn, and labzyme were significantly higher (p <0.05) in body weight and weight gain . As well as a significant decrease (p <0.05) in the amount of feed consumed and a significant improvement (p <0.05) in the efficiency of food conversion compared to other experimental treatments .2 - The first, fifth, and seventh treatments were significantly higher (p <0.05) in the mortality percent as comparing , to other experimental treatments .3 - A significant (p <0.05) increased was observed in the production index and the performance index and significantly decrease in the economic efficiency value for the second, fourth, third and sixth treatments.4 - The second, fourth, third and sixth treatments were significantly higher (p <0.05) in dressing percentage , tibia and thoracic characteristics , as well as a significant increase (p <0.05) in the weight of the cecum compared to other treatments.5 - There was a significant increase (p <0.05) in the weight of Bursa fabricius for the first, eighth and seventh treatments, as well as a H/L ratio was significant (p<0.05) decrease in the second, third, fourth and sixth compared to other treatments.6 - Total protein and globulin in plasma serum were significant (p<0.05) increased and decrease significantly(p <0.05) in the concentration of triglyceride and cholesterol in treatments second, fourth, sixth and third , compared to other treatments.7 - The superiority of the second, third, and fourth treatment was significantly higher (p <0.05) in the concentration of ALP enzyme in plasma serum compared to other treatments.8 - The third, seventh, second, fourth, and sixth treatments recorded the highest significant mean (p <0.05) of the degrees granted to them by theeEvaluator in the flavor, tenderness and juicy compared to the fifth, first and eighth treatments .9 - The second, seventh, third, fourth, and sixth treatments were significantly higher (p <0.05) in lactic acid in intestinal fluid , as well as There were significant (p<0.05) increase in digestibility of dry matter , protein and fat in treatments fourth, second, third and sixth as compared with other .10 - The fourth, second, third, and sixth factors significantly exceeded (p <0.05) in weight and length of the tibia , bone index , ash ratio , and skeletal weight of the chickens, as well as The second, fourth, third, and sixth treatments were significantly higher (p <0.05) in the level of calcium and phosphorus in the bone compared with other .11 - The first, eighth, fifth and seventh treatments were significantly higher (p <0.05) in the rate of food passage in the gastrointestinal tract compared with other.12 - Significant increase (p<0.05) in villi length and crups depth in treatments fourth, second and third as compared with seventh, fifth, eighth and first.Fourth laboratory experiment : The results of the experiment showed no significant differences in the treatment of MPK with gentamycin in diameter (mm) inhibition of Staphylococcus aureus, Escherichia coli, Which cause BCO disease , while significantly increase (P <0.05) with Pencilin, Labzyme, Tetracyclin , Biozyme in diameter of the inhibition zone to the staphylococcus aureus and the diameter of the inhibition zone of Escherichia coli.

مقارنة القيمة الغذائية لاسماك الكارب الشائع Cyprinus carpio L. من مصادر مختلفة == Comparison of nutritional value of the common carp Cyprinus carpio L. from different sources

Author name: عطور عبد الكريم طاهر العطبي
Supervisor name: امجد كاظم رسن | صلاح مهدي نجم
General topic: Agricultural sciences
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was carried out during the period from December 2015 and June 2016 to assess the chemical composition, fatty and amino acids and the total bacterial count of the muscular tissue from the common carp Cyprinus carpio L. in Basrah city which were cached from Shatt Al - Arab river at Abul - Khasib, obtained from earthen ponds at the University of Basrah, fish cages in Shatt Al - Arab river at Al - Hartha district and those imported from Iran Republic which were available in the domestic markets of Basrah city.The results of the study were statistically analyzed using CRD design applying SPSS statistical package ver. 17 on 50 fish from each source with 3 replicates per sample and the studied factors were tested using RLSD test at 0.05 probability level.The results of the statistical analysis indicated no significant differences (p>0.05) in moisture content between Shatt Al - Arab samples, imported samples, iced pond fish and iced cage fish. No significant differences (p>0.05) were detected also between fish from ponds and cages. Significant differences (p<0.05) were detected between fresh fish from ponds and cages with fresh and iced fish from Shatt Al - Arab or imported fish. The highest moisture content of fresh fish was 78.2% in Shatt Al - Arab samples while the lowest 70.8% was in fresh cage fish. The highest moisture content in iced fish 79.4% was in imported samples while the lowest 74.1% was in cage fish. As for ash content, the statistical analysis revealed significant differences (p<0.05) between imported fish and other fish samples. Significant differences (p<0.05) were observed also between fresh pond fish and other samples.However, no more significant differences (p>0.05) were detected between other fish samples. The highest ash content in fresh fish 2.29% was in pond fish and the lowest 0.98% in imported fish. Similarly, the highest ash content in iced samples 1.45% was in pond fish and the lowest 0.87% in imported samples. The statistical analysis of fat contents demonstrated significant differences (p<0.05) between Shatt Al - Arab fresh and iced samples and other samples. Fat content in pond fish was in line with those in iced cage and iced imported fish but significantly (p<0.05) differ from those in fresh pond and imported fish while no such differences were shown between pond and imported fish. The highest fat content in fresh fish 11.9% was in imported fish and the lowest 3.43% in Shatt Al - Arab fish. Similarly, the highest fat content of iced fish 9.99% was in imported fish and the lowest 3.17% in Shatt Al - Arab fish. The statistical analysis showed that protein contents in fresh fish from Shatt Al - Arab, ponds and cages were significantly (p<0.05) higher than those in imported fish while protein contents in fresh and iced imported fish were significantly (p<0.05) lower than it in other fish. The highest protein content in fresh fish 18.71% was in pond fish and the lowest 10.05% in imported fish. Similarly, the highest protein content in iced fish 16.96% was in pond fish and the lowest 9.98% in imported fish. The statistical analysis showed that the caloric values of fresh and iced fish from Shatt Al - Arab were significantly (p<0.05) lower than other fish except for iced pond and imported fish. Additionally, no significant differences (p>0.05) were observed in caloric values between pond, iced cage and imported fish while pond and Shatt Al - Arab fishes only showed significant differences (p<0.05) in comparison with other samples. The highest caloric value for fresh fish 165.62 Kcal/100 g in cage fish and the lowest 98.5 Kcal/100 g in Shatt Al - Arab fish. On the other hand, the highest caloric value for iced fish 148.4 Kcal/100 g was in cage fish and the lowest 93.9 Kcal/100 g in Shatt Al - Arab fish.The fatty acids was assessed in the laboratories of The College of Agriculture, University of Basrah by using GC - MS QP210 Ultra , SHIMADZU, JAPAN equipped with capillary column DB - MS 5 with static phase (5% phenyl, 95% methyl polysiloxane) and ultra - high purity Helium 99.9%. The fatty acid analysis for the muscular tissue of fish indicated high levels of oleic ،palmitic ،stearic ،stearic ،docosahexaenoic acid in comparison with lower levels of myristoleic ،eicosadienoic ،dihomo - gamma - linolenic and pentadecyclic acids. The statistical analysis revealed no significant differences between both fresh and iced samples from the different fish sources. However, significant differences (p<0.05) were detected between imported fish and fresh and iced fish from ponds and cages. Additionally, no significant differences (p>0.05) were observed between fresh and iced imported samples with both fresh and iced pond fish while fresh and iced cage fish differed significantly (p<0.05) from fresh pond samples.Amino acids were assessed by using HPLC system with ion exchange and Ninhydrin post column derivation in Shimadzu Spd - 6 Av uv - Visible detector. The analysis of amino acids in fish muscular tissue demonstrated high levels of Aspartic ،Serine ،Glutamic،Alanine and Glycine and low levels of Cystine ،Tryptophan ،Isoleucine ،Methionine،Histidine ،Threonine and Tyrosine. The statistical analysis showed no significant differences between fresh and iced Shatt Al - Arab samples with fresh and iced imported fish. No significant differences (p>0.05) were observed also between fresh and iced pond fish with fresh and iced cage fish. On the other hand, significant differences (p<0.05) were found between fresh and iced Shatt Al - Arab fish with fresh and iced imported samples. Additionally, another significant differences were detected between fresh and iced pond fish with fresh and cage fish.As for the total bacterial count, the statistical analysis revealed significant differences (p<0.05) between all fresh and iced fish samples. The highest level in fresh fish 7.6 x 103 cfu/g was observed in Shatt Al - Arab fish while the lowest level 2.0 x 103 cfu/g in imported fish. In comparison, the highest level in iced fish 7.44 x 107 cfu/g was also in Shatt Al - Arab fish but the lowest level 5.72 x 107 cfu/g in cage fish.

عزل وتشخيص بكتيريا . Vibrio spp من بعض الاسماك المتداولة في الاسواق المحلية لمدينتي البصرة والناصرية ودراسة بعض خواصها == Isolation and Identification of Vibrio spp. from some fish proffered in local markets of Basrah and Nasiriyah city and study some properties

Author name: عذارء عودة حسين الجبوري
Supervisor name: خديجة صادق جعفر الحسيني | منال بادي صالح التميمي
General topic: Agricultural sciences
Specific topic: Food
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The study included isolation and identification Vibrio spp. bacteria that taken from fresh and frozen fish (Tenualosa ilisha , Hypophthalmichthys molitrix, Barbus sharpey and Spondyliosoma cantharus). 210 fishes were collected from Basrah market (Big market in Basrah, Al - Ashar, Al - Tanoma and 5 mile) while 150 fishes were collected of Hypophthalmichthys molitrix, Barbus sharpeyand Spondyliosoma cantharus) from Nasiriyah market (Big market and Harj market) this collecting process of samples took a period from 02.June till 30.November - 2013.Fishes were brought to laboratory and samples were taken from it and planted directly on biological media (Nutrient agar, Blood agar, MacConkey agar and selective medium (Thiosulphate Citrate Bile salt Sucrose Agar TCBS) then the petri dishes were incubated aerobically at temperature 37cº for 18 - 24 hours. 30 positive samples were identified of Vibrios depending on colony shape, cell under microscope, catalase test, oxidase test and motility test, afer that the types were identified depending on the biochemical tests and by using API20E kit, which led to identifying two kinds of Vibrios bacteria : Vibrio cholera and Vibrio fluvialis. These two bacteria were isolated from fresh fishes more than frozen fishes.Study observed that the Vibrios isolation percentage of these two bacteria in Basrah market was 26% (15 isolates) while the isolation percentage was 21% in Nasiriyah market (63 isolates).Sensitivity and antibiotics tests were done, results showed that all Vibrios cholera bacteria had 100% resistant against Aztreonem, while some of it had low resistant which was about 45% against Amoxicillin - clavulanic Acid and Nalidixic Acid, all Vibrio fluvialis bacteria isolates had resistant against Aztreonem, Amoxicillin - clavulanic Acid and Nalidixic Acid which was 100%, 75% and 25% respectively, while all VibrioAcholera and Vibrio fluvialis isolates were sensitive 100% against Amikacin, Ampicillin, Chloram - phenicol, Ciprofloxacin, Tetarcycline, Trimethoprim /Sulpha - methoxazole.Vibrio cholera and Vibrio fluvialis bacteria isolates were 100% tolerant for acidity at pH 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0, while Vibrio cholera bacteria was 60% tolerant to the acidity at pH 4.5, and Vibrio fluvialis bacteria was more resistant to the acidity of 20% and 50% tolerant at pH 4.0 and 4.5 respectively.The study showed to the ability of Vibrio cholera bacteria to resist the salinity reached 60% and 100% at concentrations 6% and 7% respectively, but it didn't grow on concentrations 8% and 9%.Vibrio fluvialis bacteria was resistant for the salinity in all the concentrations with different percentages reached to 100% at concentrations 6%, 7% and 40% at concentration 8%, while at concentration 9% there was only one sample had resistant of 20% and it was from frozn sample of Beni fish which taken from Big market in Basrah.All isolates from different sources could grow in 25 and 37 cº and in room temperature 15 cº during doing this experiment, while some isolates of Vibrios bacteria could grow with low density in 5 cº, while it couldn't grow in - 20 cº.By using Real Time (PCR) technology for detecting hemolysim enzyme gene in Vibrio spp., that 27 samples from Vibrio cholera bacteria and 3 samples from Vibrio fluvialis bacteria had this enzyme which analyzes red blood cell (RBC) and considered one of toxins that belongs to Vibrio spp. which has a role in occurrence of disease.

تاثير فاصلة ومعاملات الري وتغطية سطح التربة في بعض خصائصها وانتاج نخلة التمر Phoenix dactylifera L. == Effect of interval and methods irrigation in addition soil surface mulching on some soil properties and production of date palm Phoenix dactylifera L

Author name: عبد الرحمن داود صالح الحمد
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in an agricultural field which located at Abu - AlKhaseeb distruct /20 Km south of Basra City , during the season of growth (2013 and 2014) on a land area about one hectare, a clay soil texture and classified as Typic torrifluvents. In order to study the effect of method and interval irrigation and mulching soil surface in some physical and chemical properties of the soil and production of date palm Phoenix dactylifera L. Hillawi c.v. The irrigation method factor included four treatments; drip irrigation method (D) and surface irrigation (S) and rotation method (DS) (rotation drip irrigation with surface irrigation in binary cycle) and tide irrigation method (T), while the mulching factor included three treatments : nylon (N) and coverage waste (W) and no mulching (no). Irrigation interval factor included three treatments are (4 ,8, 12) day. Experiment carried out in factorial Experiment with random complete block design (RCBD) with three replications, the addition of irrigation water calculated from the basin American evaporation (Evap.Pan Class - A - ), which was erected in the study site, plus 20% as leaching requirements.Below is a summary of the study results : 1 - The addition of irrigation water by the irrigation methods (D, DS, S) increase the moisture content significantly for different depths of soil compared with traditional irrigation treatment (tidal). Surface irrigation treatment shows higher values in the moisture content, followed by treatment of irrigation alternately then treated drip irrigation, irrigation with treated showed tidal lowest values. The irrigation methods (drip, drip - surface, surface) increased in moisture content values with lack of a period irrigation, especially when the interval 4 day. Moisture content of the irrigation methods treatments increased significantly values morally using coverage, particularly nylon except irrigation treatment tidal which were not increase significantly. The moisture content values increase significantly with depth for all experimental treatments, and she took to decline at the end of the first season and the second compared to the first season.2 - The treatment of traditional irrigation (tidal) showed the lowest values in the bulk density, followed by treatment of drip irrigation and treatment of rotation and then surface irrigation treatment for all depths .The results showed increased bulk density values of all treatments with depth ,also that the use of the nearby interval and coverage nylon contributed to maintaining the values of bulk density and showed superior in giving the lowest value for the density. Increased bulk density values with the progress of seasonal growth of all treatments and three depths of soil.3 - The use of irrigation methods (drip, drip - surface, surface) to a significant decrease in the values of Mean Weighted Diameter (MWD) compared with the traditional method of irrigation (tidal) and the order of the effect for the irrigation methods is S <DS <D <T. The results showed that values increase with the progress of the growing season, except for the treatment of traditional irrigation (T) with superiority of depth (0 - 30) cm on other depths.4 - The cumulative infiltration and infiltration rate Increased in the soil with the traditional irrigation treatment compared to using other methods of irrigation and the methods irrigation taken following order in the cumulative infiltration and infiltration rateS <DS <D <T. The results showed that the cumulative infiltration and infiltration rate values increased with using mulching especially with nylon and follow short - rang irrigation interval .5 - The use of irrigation methods (drip, drip - surface, surface) decreased the electrical conductivity values on different depths of soil compared with traditional irrigation treatment (tidal), which maintained a significant increase in the electrical conductivity values, especially in (0 - 30 ,30 - 60 ,) cm depth, followed by treatment of drip irrigation and surface irrigation, The rotation treatment DS showed high efficiency in salts washing rate in all depths and recorded the lowest values in the electrical conductivity .The results showed that the using of mulching, especially nylon contributed significantly in reducing the electrical conductivity values, and that the exposed treatment showed higher values, as the results showed that the using of long interval increase in the electrical conductivity values .Other than increase the electrical conductivity values of the surface depth of all treatments and it decreased significantly at both ends of the first and second seasons, compared with the beginning of the experiment except for the treatment of tidal which remained conservative values until the end of the experiment .6 - The use of irrigation methods (drip, rotation, surface) contributed to the significant increase of the values of productivity (weight, size, length, total sugars, dry weight and total yield) of Date Palm compared with the use of traditional irrigation method (tidal ) .The irrigation methods takes the following order in productivity : T <S <D <DS. The results showed that a significant increase in productivity with use short interval and the use of nylon mulching style. The results showed that increase in productivity in the second season compared to the first season.

التنوع السمكي وبعض الخصائص الحياتية في الجزء الشمالي لشط العرب وبعض مقترباته == Fish biodiversity and some biological characteristics in the Northern part of Shatt Al - Arab River and some of its reaches

Author name: عبد المحسن جعفر عبد الله
Supervisor name: ساجد سعد حسن النور
General topic: Agricultural sciences
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The aimed of the present study to find out the nature of the qualitative compositionand some biological aspects in the North part of Shatt Al - Arab River and some of its reaches after emergence of many changes in the region and habitats segmenting by Dam construction on Euphrates River in the area between Chibyish and Mudina town and establishment of Qlat - Salih Regulator, reduction of water discharge to minimum limits, entry of alien species on an ongoing basis, so it is necessary to conduct a study to find out the nature of the diversity of fish in the region, and it has shown that the composition of community differs from previous studies revealed that introduced alien small size species and some of uneconomic species were dominate. The samples were caught monthly from the river from December 2013 to May 2015 North of Basrah Province. Several fishing tools were used to collect fishes seine, fixed, draft, cast net and electrofishing. Three stations were selected; the station 1 was in the North of Qurna town about five kilometers North the point which the Tigris and Euphrates River confluence, station 2 at the place of Seweeb River is meeting the Shatt Al - Arab Rive and station 3 at the point that Al - Shafii River which meet Shatt Al - Arab river. Some environmental parameters were measured, namely air temperature ranged from 15ºC in December 2014 in station 3 to 43ºC in the August 2014 at station 2 and 3, while the water temperature from 11ºC in January 2015 for station 1 to 36 ºC in July at station 2. Light penetration revealed 33 cm in April and May 2014 and 132 cm in January 2015 for station 3. Current speed ranged between 0.03 m/s in January 2015 in station 3 and 0.29 m/s in October 2014 for station 1. Salinity ranged from 0.66 g/L in January 2014 for station 1 to 1.46 g/L in February and March 2015 in station 1 and October 2014 for station 2. The pH values tend toward the alkaline, the lowest 7.30 in May 2014 for station 2 and 8.90 measured in February 2014 in station 1. Monthly discharges, average incoming from the Tigris River recorded 35.20 ± 8.47 m3/s in November 2014 to 73.85 ± 17.55 m3/s on February 2014. Lower values of reactive nitrate were recorded 0.69 μ g at. N/L in March 2015 at station 3 and highest 24.32 μ g at. N/L in May for station 2, however the values of reactive phosphate recorded, the lowest 0.0037 μ g at. N/L in March 2015 at station 2, the highest was 0.589 μ g at. N/L in November 2014 for station 1. A total of 29405 individuals was collected during the study period represented 916521g included 33 species eight of them were marine species contain 29 genera belong to 14 families of Osteichthyes, Cyprinids family occupied first degree in the number of species (13) and genera (12), the numerical relative abundance of marine species in the study area was 3.38% and the relative abundance gravimetric was 8.45%. A significant positive correlation was found between number of species and water temperature (r=0. 506). Liza abu, Carassius auratus and Coptodon zillii were the most total numerical relative abundance 30.89, 29.30 and 15.64 % respectively, C. auratus, S. triostegus and C. zillii were the most total weight relative abundance and composing 27.58, 14.27 and 11.64% respectively in the study area. Discharges rates flowing from the Tigris River revealed negative effect with the number of species and the insignificant negative correlation was recorded (r= - 0.109) with discharge monthly rates, and fluctuation relationship was noticed between discharges and ecological indices. However, native species included 15 seasonal species 4 and 14 for rare species. Diversity index (H) ranged between 0.91 in January 2015 at station 3 to 2.05 in March 2015 for station 2, richness index (D) 0.83 in February 2014 at station 3 and 2.79 in March 2015 at station 1, evenness index (J) recorded 0.38 in January 2015 and the highest 0.78 in September 2014 for station 1. The percentage of similarity among stations by Jaccard similarity index (Ss%) showed variation uneven during study months. Cluster analysis of species similarity pointed to clumping in groups controlled by temperature. The Multivariate analysis was (CCA) adopted to clarify the impact of a biotic factors on the distribution of species. Total catches were ranged between 16380 g in December 2013 to 80309g in December 2014. The total average catch per unit of effort was 24.953 kg/hr for seine nets ranged from 0.928 kg/hr in December 2014 at station 2 to 30.271 Kg/hr during January 2015 for station 3. The number of fishing boats in the study area 143 their length between 5 - 7m most of them without an engine. The impact of alien species direct effects during the destruction of nests and reproductive ground, especially eggs which lie on a plant for native species and hybridization between alien and native species, and indirect effects of competition on food, ecological space, environment destruction and aggressive behavior. The length groups of all fish species were recorded, the lowest values were for Alburnus mossulensis, Acanthobrama marmid, Hemiculter leucisculus and Garra rufa, they were 7.3 - 18, 6.3 - 17, 7 - 17 and 7.2 - 17.8 cm respectively, whereas the highest were for Silurus triostegus, Leuciscus vorax and Mastacembelus mastacembelus, they were 10.8 - 72.4, 9.5 - 57.3 and 14 - 55 cm respectively. Faben method used for estimating growth parameters (L∞,K) by applying FiSAT program for C. auratus, Carasobarbus luteus and L. abu, the values of L∞ for three species 38.97, 35.69 and 22.19 cm respectively, and for K 0.29 to the C. auratus and C. luteus and 0.41 for L. abu, the results revealed five years age for C. auratus and C. luteus whereas four years age for L. abu. The present study showed high values of diet overlap between L. abu and Cheon subviridis 09% also high values 90% Oreochromis aureus and Oreochromis niloticus S. triostegus and L. vorax 89%, but it did not reach the competition limit, also high diet overlap 86% and competition was found between C. auratus and C. luteus Absolute fecundity for C. auratus females ranged from 1303 for 8.8 cm total length and 10g weight to51245 egg for 30.1 cm total length and 557g weight, and for C. luteus from 2098 for 13.1 cm total length to 14147 egg for 20.9 cm total length and 131g weight, while in L. abu from 6247 for 9.7 cm total length and 11g weight to 41219 egg for 17.5 cm total length and 63g weight.

تاثير رش التربتوفان والكلايسين واضافة المحفز الحيوي Biohealth للتربة في نمو وحاصل بعض اصناف البطاطا SolanumtuberosumL في الاراضي الصحراوية == EFFECT OF SPRAYING TRYPTOPHAN, GLYCINE AND SOIL APPLIED BIOHEALTH ON GROWTH AND YIELD OF SOME POTATO (SolanumtuberosumL.)CULTIVARS IN DESERT LANDS

Author name: عباس كاظم عبید عباس
Supervisor name: عصام حسین علي الدوغجي | نوال مهدي حمود
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Two experimentswere conducted during autum seasonsof 2013 - 2014 and 2014 - 2015 in Tomato Development Project belong to Basrah Agricultural Director, Khor Al - Zubairin southern Iraq to study the effect of foliar application of tryptophan, glycine and soil appliedBiohealthongrowth and yield of some potato cultivars. The aim of the experiments was focused on the effect of three potato cultivars (Draga, Arnova and Provento) in the first season and (Arizona, Arnova and Rivira) in the second season.Spraying with two concentrations( 100 and 200 mg.l - 1)for both Tryptophan and glycine and 0 mg.l - 1(sprying with distilled water).Spryingwas done twice 45 and 60 days after after propagating.Besides, additiontwo concentrations ofBiohealthinto the soil( 0 and 1 g.l - 1for the first seasonand 0 , 1 and 2 g.l - 1 for the second season). The addition ofBiohealthwas done twice 45 and 60 days after propagatingfor both seasons. The experiments was adopted as factional experiment.Split - Split Plot Design was used with three replication,Least Significant Differences Test(LSD) was used at probability of 0.05. The results could be summarized as follows : 1. Emergence of field parameters Results showed that"Arnova" cultivargave asignificant increases in speedemergence field after 20.51 and 19.43 daysin both season,respectively."Brovento"in first season and “Arezona” in the second seasongave asignificant increasesin the percent of field emergence 96.42 and 89.33%respectively, .2.Vegetative growth parameters Resultsshowed that"Draga"cultivar in the first season and "Rivira" inthe second seasonincreased significantly in mostvegetative growth parameters.Spryingwith 200mg.l - 1 tryptophan in bothseasons gave increased significantly in mostvegetative growth parameters.Application withbiohealth1 g.l - 1 in the first seasonand 1, 2 g.l - 1In the second season gave significant increases in mostvegetative growth parameters.The interactions between the studied factors caused significant increased effects in all vegetative growth parameters on both seasons. 3. Yields parameters Results in the first season showed that "Draga" cultivar gave significant increases in tuber number.plant - 1,total plant yield, marketable yield,total yield, small and large tubers yield (6.10 tuber,379.9g,3.36 ton.donum - 1, 3.71 ton.donum - 1,0.35ton.donum - 1 and2.52 ton.donum - 1,respectively), While "Rivira cultivar in the second season gave significantincreases in tuber weigh,total plant yield,marketable yield,total yieldand large tubers yield(88.38 g, 492.6g,4.50 ton.donum - 1,4.82 ton.donum - 1, 3.87 ton.donum - 1,respectively).Sprying with 200 mg.l - 1 tryptophan in the two seasons gavesignificant increases in tuber weigh (69.08, 74.95 g), total plant yield (389.1 , 438.4 g), marketable yield (3.54, 3.87ton.donum - 1),total yield (3.80, 4.28 ton.donum - 1) and large tubers yield(2.44, 3.08 ton. donum - 1) respectively, While the 100 mg.l - 1 tryptophan increases in tuber number.plant - 1 in the first season(5.76 tuber) and 200 mg.l - 1 tryptophan increases in the first seasonin yield medium tubers (1.10 ton.donum - 1)and in the second season increases in tuber number.plant - 1(5.90 tuber).Application with1g.l - 1 biohealthin the first season gave significant increases in tuber number.plant - 1,total plant yield,marketable yield,total yield,medium and large tubers yield(5.72 tuber, 366.6 g, 3.28 ton.donum - 1,3.59 ton.donum - 1,1.03 ton.donum - 1and 2.26 ton. donum - 1,respectively), while in the second season application with 2 g.l - 1 biohealthgave significant increases in tuber number.plantC 1,total plant yield,marketable yield,total yield,and large tubers yield(5.85 tuber, 423.4 g), 3.75ton.donum - 1 ,4.15 ton.donum - 1 and 3.00 ton.donum - 1,respectively). The interactions between the studied factors caused significant increased in allyields parameters on both seasons.4. Chemical characteristics 4 - 1. Leaves chemical characteristics Spraying with 100 and 200 mg.l - 1 tryptophan in Carbohydrate contents. In the second season200 mg.l - 1glycinegave significant increases in carbohydrate contents.Application with 1 g.l - 1biohealthin second season gave a significant increase incarbohydrate contents.The interactions between the studied factors caused significant increased effects in all leaves chemicalparameters on both seasons.4 - 2. Tubers chemical characteristics Results showed that "Brovento" cultivar in the first season gave significant increases in the percentage of dry matter, starch and specific gravity, "Draga" increases in thepercentage of protein, vitamin C and total solubilesoilds, "Arnova" increases intotal free amino acids. In the second season "Rivira" cultivar gave significant increases inthe percentage of dry matter, starch, specific gravity and total free amino acids and"Arezon" increases in thepercentage of vitamin C and total solubilesoilds.Foliar application with tryptophan at 200 mg.l - 1 in the first season caused an increase in the percentage of dry matter, starch,specific gravity and protein, while 200 mg.l - 1 glycine increases in vitamin C and total solubilesoilds, glycine at100 mg.l - 1increases in total free amino acids.Foliar application with tryptophan and glycine at 200 mg.l - 1in second season caused an increase in the percentage of dry matter, starch andspecific gravity, while 200 mg.l - 1 glycine caused increases in the percentage of protein and total free amino acids, glycine at100 mg.l - 1 increasesin vitamin C and tryptophan at 200 mg.l - 1 in total solubilesoilds.Application with biohealth gave asignificant increase in mosttubers chemical characteristics for both season.The interactions between the studied factors caused significant increased effects in all tuber chemicalparameters on both seasons.

استخلاص وتنقية وتوصيف متحللات بروتينية من مخلفات الاسماك والروبيان واختبار كفاءتها في حفظ اقراص مفروم اللحم البقري == Extraction, purification and Characterization of Protein hydrolysates From Fish and Shrimp by - Products and Assay Efficiency in Beef Patties Storage

Author name: عالية زيارة هاشم الحلفي
Supervisor name: ام البشر حميد جابر الموسوي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was interested in the preparation of bioactive peptides by using shrimp and fish by products.Three types of proteolytic enzymes , Alcalase and Pepsin and Flavourzyme were used for this purpose.The constituents (moisture , protein , fat and ash) of defatted raw and dried materials were studed .The proteolysis action of the three enzymes was observed for 5 hours the peptides chain length of the protein hydrolysates were determined and tested for their antioxidat and antibacterial 240 minutes of enzyme assay was reliance according to the highly antioxidantive and antibacterial properties of produced peptides.The proteolysis of shrimp by - product by Alcalase and Pepsin gave highly antioxidantive activity. The isolation , purification and the peptides bioactivity determination was achieved as below : 1 - The peptides of the both protein hydrolysates were isolated by using Ultrafiltration membranes of 5 MWCO KDa. The isolated peptides were examined for their antioxidative activity. It shown , that the peptides wjich was synthesized by the proteolytic activity of Alcalase has ahigher antioxidative action (53.67%) comparing with pepsin peptides (41.19%) at concentration of 100 mg/mL. The results showed that peptides of hydrolysate enzyme Pepsin give inhibitor zoon against bacteria test in inhibition zoon ranged from 10 - 11 mm and determined peptides content of amino acids and showed glysin , threonine ,valine and lysine, which amounted to 9.11% and 8.94% and 7.51% and 9.16%, respectively in peptides of enzyme Alcalase while recorded amino acids serine 9.69% ,theronine 8.76% ,cystin 14.10% and 7.06% lysine ratios highest peptides hydrolysate enzyme Pepsin respectively.Summaryb2 - purification of peptides by gel filtration was showed four peaks of peptides hydrolysate enzyme Alcalase and three peaks for peptides hydrolysate Pepsin enzyme and tested the antioxidation activity of all peaks and inhibitory to bacteria, which recorded that second peak of the Alcalase enzyme antioxidant activity amounted to 63.28% and peptides The first peak of the same enzyme%48.57 the peptides first and the second peak for hydrolysate Pepsin enzyme was% 41.65 and 55.21% also tested the inhibitory effect against some types of bacteria have been to peptides second peak of the Pepsin enzyme hydrolysate towards the bacteria Escherichia coli, Salmonella typhi, Staphylococcus aureus, Bacillus cereus , Pseudomonas aeruginosa inhibition zoon ranged 13 - 9 mm, and determined the minimum inhibitory concentrations (MIC) of these peptides and found that the concentration of 125 and 250 mg / ml values have affected all types of bacteria testing. The cellular toxicity of peptide peakes in analysis of human red blood cells, and has not any toxic effect observed for all concentrations and different periods incubation of peptides peasks.3 - The molecular weight of peptides two of peak enzymes was determined by electrophoresis and cleared two bandes each peak represents two chain peptide molecular weights 3.71 and 4.37 KDa the first peak and the second peak 3.71 and 4.27 KDa of the hydrolysate Alcalase enzyme and chains peptides first peak of hydrolysate Pepsin enzyme 3.71 and 4.16 KDa the second peak, 3.71 and 3.98 KDa, respectively , The study included determined of amino acids and found that it contains all the amino acid varying percentages depending on peptides peakes hydrolysate protein.4 - The stability of antioxidant peptides towards thermal treatment to different degrees thermal ranged between 25 - 100C and change in pH 2 - 11 and treatment of sodium chloride salt ranged between %2 - 8 was studed and found that the antioxidant activity of peptides and reducing power stable at 60C The ability to binding ion ferrous ,hydrogen peroxide , hydroxyl radical scavenging and superoxide anion scavenging they appear stable at thermal 40C and whenctesting the stability of peptides to change the pH was observed that the peptides two peakes hydrolysate enzyme Alcalase stable at pH 8 but decreases when moving away from this value towards the basic or acidic while shwoed peptides two peakes hydrolysate enzyme Pepsin stability at pH 7, and this stability declined when number pH to 11, and when the treatment with salt sodium chloride showed peptides first peak and second enzyme Alcalase and Pepsin stable antioxidant activity at 4% salt concentration for all tests except the oxidation stability of peptides in binding ferrous ion has showed at 6% salt concentration.5 - when the follow of peroxide values for beef patties treated with peptides two peakes enzymes, The results showed there is adecrease in peroxide values for beef patties treated with peptides of the Alcalase enzyme was more cleared compared to the peptides of first peak for the same enzyme and peptides two peakes Pepsin enzyme also got a decrease in the total number of bacteria and total coliform bacteria and psychrophilic bacteria When the treatment second peptides peak of the Pepsin enzyme concentration of 50 and 100 mg / 100 g meat.6 - Sensory evaluation of beef patties showed that the treatment samples with peptides second peak of the Alcalase enzyme recorded the highest degree of sensory evaluation peptides compared with the first peak of the same enzyme and peptides peaks hydrolysate Pepsin enzyme.

انتاج مشتقات الكايتوسان الذائبة بالماء والمستخلصة من قشور الروبيان بتفاعلات ميلارد وتطبيقاتها في بعض الانظمة الغذائية == Production of Water soluble Chitosan derivatives extracted from shrimp shells by Maillard reaction and their application in some food systems

Author name: عاليه جميل علي
Supervisor name: علي حسين عبد الكريم العامري | منير عبود جاسم الطائي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study included the preparation of of chitin from shrimps shells Penaeus semisulcatus and studied of chemical composition of it like moisture,ash and protein wich was 7.80,0.43and3.50%respectively,The yield was 20.0%,then prepared three different types of Chitosan A,B and C from extracted chitin by removing of acetyl group from chitin at different times 4,10 and 20 hour, determination degree of deacetylation for types of Chitosan and standard Chitosanby FTIR 72.30,83.60,98.50 and85.20% respectively as was the viscosity of the three types of Chitosan 137.70,125.21 and74.11 centipoise respectively, selected Chitosan C,which carries ahigher valuedegree of deacetylation 98.50% and studied physico chemical and functional properties,which included (yield ,moisture , ash, protein and solubility) which amounted to16.20, 5.50, 0.20, 1.15 and 98.90%, respectively and reached a molecular weight (17.782) kilo daltone.Capacity of Chitosan to bind fat FBC and water WBC by using three types of oils are (olive oil, corn oil and sunflower oil) were estimated and were givin the highest capacity link with olive oil 665.0%, while the ability to bind Water amounting to 772%, as measured X - ray diffraction (XRD) and use the scanner electron microscope (SEM) to see morphological Chitosan and standard Chitosan Chitosan and the standard Chitosan, while the profile of the thermal gravimetric analysis (TGA) for Chitosan and standard Chitosan show that highest thermal decomposition at 308.56 ,315.92ᵒc respectively .The water soluble Chitosan derivatives were prepared by reaction with five types of reducing sugars by Maillard reactions and the use of three treatments,first treatment at a temperature of 50ᵒc for 1 - 7 days, the second treatment at a temperature of 100ᵒc for 1 - 7 hours and the third treatment at a temperature of 121ᵒc for 1/4 hour by autoclaving, ,and the properties of these derivatives were studied estimated yield, solubility, stability, free amino groups and the change in reducing sugars. highest value of yield gavein by The third - treatment, second treatment, first treatment reached 45.20,43.50,040.0% either solubility reached 1.10 ,0.780, 0.82 g/100 ml and derivatives stability at pH between10.50, 10.0, 9.80 respectively, The largest amount of amino groups and reducing sugars was the first treatment at absorbance 0.590 and0.352 respectively while the second an third treatment amounting to (0.201 and 0.060) respectively for amino groups , and 0.400 and 0.100 respectively for reducing sugars.Properties of antioxidants for prepared Chitosan and its derivatives. The antioxidant activity of Chitosan ranged between17.8 - 73.2%, while the highest activity for the third treatment of derivatives, the second and the first ranged of 76 0.81 - 85.88, 71.30 - 82.70 and 66.01 - 78.50% respectively, The reducing power of chitosan ranged between 0.320 - 0.700, while the highest value of absorbence of reducing power of derivatives to the Third treatment and then the second and finally the first which amounted to 1.290, 1.201 and 0.693 respectively, while the ability of chitosan to chelating ferrous ion and scavenging hydroxyl radical range between27.60 - 72.99%and16.90 - 84.9% respectively.Thehighest percentage to scavenging hydroxyl radical and ability to chelating ferrous ion by derivatives is shown by the third the second and the first treatment reaching 89.98 and 82.8 ,88.80 and 81.90, 84.80 and 76.01 % respectively, the selected third - treatment derivatives (121ᵒc for 1/4 hour) because of its best properties and carried the tests such as analysis of thermal gravimetric (TGA) that was noted three stages to different degrees heat to the disintegration of the derivatives, and the detection of toxicity on human bloodThird treatment compounds identified by GC - MS and the results showed that the derivatives have defferant compounds ,and identified compounds name, molecular formula, structural formula, molecular weight, size and retention time in addition to the profile mass spectrometerIn the practical side the use prepared chitosan in increasing storage time of table eggs, physicochemical tests were carried out for eggs stored at a temperature of 15 ᵒc and for aperiod of six weeks, it included estimation of weight loss (WL) wich amounted to 3.36% for chitosan at the six week as compared with acetic acid and control sample wich 9.01 and 8.96% respectively.Value of yolk Index YI of Chitosan - coated eggs decreased at the sixth week, amounted to 0.35, as compared with acetic acid and control sample wich reached (0.19 and 0.22) respectively, The value of Haugh Unit HU in the sixth week was higher to Chitosan treatment wich amounted to 61.42 compared with acetic acid treatment and control sample wich is24.95 and24.37 respectively.As regards eggs grading EG Chitosan treatment ongradiation A up to fifth week while acetic acid treatment and control sample reached gradiation C , when estimating pH of egg albumin pH high increased has been noted in control and sample treated by acetic acid in compared with chitosan - coated sample wich ranged 9.30, .Chitosan derivatives were also used chitosan as - natural antioxidants in beef products through the follow - up assessment peroxide value at different storage time the highest concentration 0.11g/100g beef meat displays the highest activity to prevent oxidation oil of beef meat wich reached5.11.Asensory evaluation has been carried of product stored on a 4ᵒc for 5 days of recipes sensory color,flavor,juiciness, tenderness and public acceptance using different concentrations.They have shown significant deferences ,but concentration at (0.11g/100g beef meat)was superior over the other concentrations and samples treated with BHT

تاثير التقليم الورقي والرش بالاثيفون في صفات الثمار الفيزيوكيميائية والفسلجية والتشريحية والحاصل في نخيل التمر Phoenix dactylifera L. صنف الحلاوي117 == Effect of leaf pruning and ethephon spray on fruit physico - chemical , physiological , anatomical and yield of Phoenix dactylifera L., cv. Hil

Author name: ظافر ناصر عبود خلف القطراني
Supervisor name: علي حسين محمد الطه
General topic: Agricultural sciences
Specific topic: Plant - Gardening
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted in a private orchard at Abi El - Khassib district , Basrah Governorate, during the growing season of 2014 to investigate the effect of leaf pruning at levels of 6 , 9 and 12(leaf . bunch⁻¹)during the onset of pollination and spraying ethephon on bunches at concentrations of Zero , 250 and 500 (mg.L⁻¹) , once, at Kimri stage on fruit physico - chemical , physiological , anatomical characters and yield of date palm ,cv. Hillawi . Results showed that leaf pruning treatmeats of 9 and 12 leaf . bunch⁻¹ recorded significant increases in fruit , pulp fresh weight , fruit size , length and diameter and water content of fruit at Rutab stage, carotene pigment concentration of fruit skin and respiration rate of fruit at Kimri and Khalal stages, ripening percentage at Khalal and Rutab stages, bunch weight of tree at Tamir stage . Leaf pruning treatments of 6 and 12 leaf.bunch⁻¹ increased total chlorophyll pigment concentration of fruit skin at Kimri stage significantly. Leaf pruning treatment at 12 leaf.bunch⁻¹ recoreded significant increases in cell length and width of inner mesocarp of fruit at Khalal stage, and total yield of tree at Tamir stage. Treatments of 6 and 9 leaf.bunch⁻¹ increases significantly total chlorophyll pigment concentration of fruit skin and number of cells in mm² of inner mesocarp of fruit at Khalal stage, whereas treatment at 6 leaf.bunch⁻¹ increased fruit dry matter percentage and total acidity and respiration rate of fruit at Rutab stage. Ethephon treatments at concentrations of 250 and 500 mg.L⁻¹ had significant increases in fresh weight of fruit at Rutab stage, and respiration rate of fruit at both Kimri and Khalal stages. Treatment of 500 mg.L⁻¹ ethephon recorded significant increases in pulp weight of fruit , fruit volume , length , diameter , total soluble solides , phosphorus concentration of fruit at Rutab stage, and carotene pigment concentration of fruit skin at Kimri , Khalal stages, and ripening percentage of fruit at Khalal and Rutab stages, and cell length and width in inner mesocarp of fruit at Khalal stage, and bunch weight and total yield of tree at Tamir stage. Both treatments of Zero and 250 mg.L⁻¹ ethephon recorded significant increases in total chlorophyll pigment concentration of fruit skin at Kimri and Khalal stages, and number of cells in mm² of inner mesocarp of fruit at Khalal stage, whereas control treatment of ethephon increased fruit total acidity and respiration rate significantly at Rutab stage. Bi - combination of leaf pruning at 12 leaf .bunch⁻¹ and spraying ethephon at concentration of 500 mg.L⁻¹ recorded significant increases in most of the studied characters as compared to other bi - combination treatments

معايرة معادلات التبخر نتح المرجعي لمنطقتي الفرات الاوسط وجنوب العراق وتاثير جدولة الري والتسميد في بعض خصائص التربة الفيزيائية وكفاءة استعمال المياه لمحصول زهرة الشمس Helianthus annuus L) == Calibration of Reference Evapotranspiration Equations in the Midmost Euphrates Region and Southern Iraq and the effect of irrigation schedule and fertilization on some Soil physical properties and water - use efficiency of sunflower crop (Helianthus annuu

Author name: طالب صبر حريجة العسكري
Supervisor name: داخل راضي نديوي | محمد عبد الله عبد الكريم
General topic: Agricultural sciences
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Theoretical foundations for research included the estimations of reference evapotranspiration (ETo) in the midmost Euphrates region and southern Iraq by applying seven mathematical models as well as CROPWAT 8.0 and CLIMWAT software using two sets of climatic data for periods 1970 - 2002 and 2004 - 2010.The results could be summarized as follow : - 1. The annual mean of ETo calculated by the Penman - Monteith equation (PM) for the meteorological stations of Nasiriya, Amarah, Semawa, Najaf, Diwaniya and Kerballa were (6.00, 6.20, 5.92,5.97,6.13,5.74) mm day - 1 respectively, during the first measurement period, and (6.47, 6.08, 5.86, 5.98, 6.13, 5.65) mm day - 1 respectively, during the second period. Also, the ETo estimated by CROPWAT 8.0 and imported by CLIMWAT software have showed the same trend of PM calculations with overestimate reached (6.10, 5.93) % during the first and secondperiods for CROPWAT 8.0 software, and underestimate for ETo reached 8.16 %during the first period for CLIMWAT software compared to PM calculations.2. The statistical criterions for evaluating the performance of six empiricalequations of the original versions were compared with the Penman - Monteithequation revealed that the variations in their performance with relativelypreference for temperature - based equations in comparison with radiation - basedequations. Blancy - Criddle (1950) equation (BC) has proved superiorperformance in Nasiriya, Semawa, Najaf, Diwaniya and Kerballa stations duringboth periods, also in Amarah station during the second period and declining itsperformance for the benefit of Kharrufa equation (1985) (K) during the firstperiod of measurement.3. The calibration process of the six empirical equations including : Hargreaves - Samani (1985) (HS), Makkink (1957) (M) , Priestley - Taylor (1972)(PT),andHargreaves (1975) (H) in addition to BC and K equations were made bysubstituting recalibrated constant values for the original constant values afterthese constant had modified to fit PM calculations and it used a set of statisticalcriterions to verify the validity of calibration and its ability to reduce theestimates errors compared to the original formulas. As the indices ofconcordance (D), confidence (C), correlation (r) and regression (R2) togetherwith the Nash - Sutcliffe Efficiency(NSE), standard deviation ratio (RSR). meanabsolute error (MAE), root mean square error (RMSE), relative error (RE)indicators and the ratio between both average estimations of ETo (r) are used toassess the validation of calibration and optimization which revealed thatconfidence became high in modified equations with reducing the estimation errorof ETo due to approach the values of these criterions from optimal value, then,they had improved efficiency of the six equations to simulate the values of EToare more accuracy than original formulae.4. The proposed new constants for the estimation of the ETo with : BC was 1.37for the time periods from January to April and 1.39 for May to December insteadof the original constant 1 : 41, HS was for the time periods from Octoberto March and for April to September instead of the original constantb0.0023, K was for June - July and for remained months instead of theoriginal constant 0.34, M are for the time periods from November to Mayand for June to October instead of the original constant 0.61, the equationsPT and H were 1.96 and 0 0.0165 for the period of December - May and 2.61and 0.0178 for the period from June to November instead of the originalconstants of 1.26 and 0.0135 respectively and equationsThe side applied to the study has included the implementation of the fieldexperiment for the cultivation of sunflower crop through the loop spring 2013 inprivate farm lies according to the geographical positioning system (GPS) withinthe coordinates (606,366.3437642), (606320, 3437729), (606353, 3437749),(606400, 3437660), is located in Batah town, Thi Qar province, at a distance of 17km west of the city of Nasiriyah, up 5 meters from the sea surface. The mainobjective of experiment included the following : i. Measuring ETo for the study through the crop growing seasonii. To determine the crop coefficient (Kc) and the actual water consumption(ETa) of sunflower crop in the Batah areaiii. . To asses effect of irrigation scheduling and fertilizer application as well astheir combinations on the growth indicators, nutrient uptake, dry matter,crop yields, the crop water use efficiency and water productivity.iv. Evaluating impact of irrigation scheduling and fertilizer application besidestheir combinations on some of soil physical properties at the end season.A field experiment was carried out using factorial experiment in a randomizedcomplete block design (RCBD) Layout with three replications in two factors, nineirrigation Scheduling treatments were assigned to the main plots and four fertilizertreatments were assigned to the subplots. Sunflower seeds (flame) were planted onMarch 23 with plant density 61538 plant ha - 1; the harvest operation was conductedon 18 / July / 2013.The irrigation Scheduling was achieved based on replenish root zone to theborders of field capacity when it reach the percentage of management allowabledepletion (MAD) identified about 55% of total available water (TAW), taking intoaccount the diversity of the effective depth of the root zone according to the stagesof growth, . The experiment involved Irrigation scheduling of the sunflower basedby calculating ETa using Kc which adjusted in situ multiplying by ETo thatestimated by PM equation (S1) ,original BC equation (S2) and adjusted K equation(S3) using meteorological data for real time that collected from agrometeorologicalstation nearby the field. PM equation (S6) using historicalmeteorological data and from pan evaporation with applied (FAO 56, 1998) tocalculate pan coefficient (S4). Whereas, irrigation Scheduling based on soil waterbalance according to daily measurements of soil moisture and adjusted daily onthe basis of changes in moisture content (soil water balance) (S5), fixed irrigationinterval and variable irrigation depth (S7), different irrigation interval and variableirrigation depth (S8) and soil water balance for bare soil (S9). Also the studyincluded four fertilization treatments were used ( F : mineral fertilizer, C : Compost,M : mineral fertilizer + Compost , N : non fertilization). The results showed thefollow : c1. The maximum value of ETo during season growth of sunflower crops accordingto field measurements has increased significantly when using evaporation pan,which gave the highest average rate of 8.81 mm day - 1 in comparison with PMequation that recorded the lowest value of ETo with mean equal to 8.00 mm day - 1,while BC and K gave 8.66 and 8.14 mm day - 1 respectively.2. The converged seasonal mean of Kc value for sunflower crops during growthseason were 0.864, 0.862, 0.863, 0.861, 0.862, 0.862, 0.861, 0.862 for S1 - S8treatments respectively, while the Kc varied in different stages of growth, thehigher value of Kc was 1.213 observed during the flowering stage, followed bytwo phases of vegetative growth and maturity with mean 0.796, 0.494respectively, whereas the lowest values (0.350) was recorded in the initial stage3. The ETa values were variable with various irrigation Scheduling treatments,the treatments (S1 - S8) gave mean ( 876.86, 938.98, 885.57, 943.40, 898.47,949.05, 835.61, 839.76) mm Season - 1 respectively, the mean values of ETa aredistributed as 52.28% during flowering stage, 23.38% through maturity, 18.97%during vegetative growth stage and 5.36% in the initial stage4. The Irrigation scheduling of the sunflower based on S5 treatment caused toincrement of water consumption by transpiration during flowering stage to reach83% from total ETa depth, whereas The Irrigation scheduling based on S7treatment caused to increment of water consumption by evaporation during thesame stage to reach 29% from total ETa depth.5. The results demonstrated that all Irrigation scheduling have been showed astrong relationship with water balance measurement (S5), by R2 is greater than0.91 and r greater than 0.95, also, the results showed there was a preference to therevised K equation to calculate ETa of sunflower crops in the region according toindicators of regression model each of the R2, slope and intercept which amountedto 0.9395, 0.9875, - 0.0144 respectively.6. The overall mean of net irrigation depth was 874.6 mm season - 1 ranged from941 mm season - 1 for S4 treatment to 800 mm season - 1 for S7 . the addition ofwater was 14 applications for S1 - S4 treatments and 15, 13, 12 applications for S5,S8, S7 respectively.7. The lowest nitrogen concentration in the leaves of Sunflower crops duringflowering stage related to decrease water supply at S7 (27.69 g kg - 1) , then, itincreased to maximum value ( 31.48 g kg - 1) when irrigation Scheduling based onS1 followed by S3, S5, S2, S4 treatments,. As respect with maturation stages theS5 treatment gave maximum mean (26.81 g kg - 1) was significantly superiorcompared to all treatments except S1 and it gave increment in nitrogenconcentration equal to 22% in comparison with S8 which recorded lowest nitrogenconcentration (21.94 g kg - 1). The results showed the F treatment was significantlysuperior followed by M treatment which gave average 34.43, 33.42 g kg - 1 duringflowering stage and 28.69, 26.48 g kg - 1 during maturation stage respectively.8. Increasing irrigation depth due to an application S4 and S6 treatments andIncreasing the irrigation frequency for S5 treatment led to significant increment inthe bulk density for 0 - 15 cm depth with mean 1.393, 1.384, 1.386 M m - 3respectively, the bulk density decreased steadily with decreasing of irrigationddepth and irrigation interval until reach their lowest level (1.350 M m - 3) at S7treatment. The bulk density was significantly decreasing when C and M treatmentswere applied with means of equal to (1.362, 1.364) M m - 3 respectively, atmagnitude 1% higher than their initial values. Also the results showed theirrigation scheduling treatments and fertilizer application as well as theircombinations failed to exhibit any significant influence on bulk density for depths15 - 30 cm and 30 - 45 cm.9. The results showed a significant increase in the soil penetration resistance forsoil with high watery supply (S4 and S6), while that status is significantlydecreased under treatment of low watery supply (S7, S8 and S1), the soils withmedium watery supply causing the pattern moderate between the two, also, thisepithet is significantly reduced when you add compost with an average of 708.09kPa, while there was a significant increase resulted from control treatment andaddition of chemical fertilizer alone, the proportion of 31.81 and 18.41%sequentially compared with the addition of compost.10. The results showed the possibility basic infiltration rate for most treatmentsunder the category (moderately slow) according to Landor (1991), by the mean ofbasic infiltration rate was 1. cm h - 1 ranged between 0. to 3. cm h - 1, whereasbasic infiltration rate for S4N and S9 decreased to 0.6 , 0.51 cm h - 1 respectively,thus it can be classified into (slow) while it increased greater than 2.0 cm h - 1 forS1C, S1M, S2M, S5C, S7F, S7M,S7C, S8M treatments which refer to moderateinfiltration rate according to the same classification . C and M fertilization led toimprove soil physical properties, which in turn positively affected the infiltrationrate with increment in magnitude (5 , 56) % respectively, compared to Ftreatment.11. Treatment of F gave a significant superior in most morphologicalcharacteristics and yield components, the efficiency of F treatment for productionwas 37.45 % followed by M and C treatments were achieved 24.66, 19.24 %respectively, compared to N treatment.12. Treatment F achieved highest yield of grains 1945.80 kg ha - 1, an increasing was24.32% in comparison with N treatment which gave lowest yield of grains reached1367.24 kg ha - 1, also there were a significant increase in crop water use efficiencyand water productivity are associated with application of F and M fertilization13. The productivity characteristics of sunflower were significantly affected byirrigation Scheduling treatments, The highest seed yield 1893.8 kg ha - 1and thehighest dry matter 5246.2 kg ha - 1were obtained under S5 treatment, while the S7treatment recorded lowest values of these indicators (1512.5 and 3765.4) kg ha - 1 respectively,14. The application of Irrigation scheduling based on S5, S1 and S3 treatments achieved a significant increase in crop water use efficiency and water productivity, the mean of first parameter was (0.219, 0.216, 0.216) kg m - 3 respectively, and themean of the second parameter was (0.228, 0.220, 0.219) kg m - 3 respectively.

دراسة بعض الجوانب الحياتية والبيئية للروبيان الشرقي Macrobrachium nipponense (De Haan, 1849) وطرائق تربيته واكثاره في البصرة، العراق == Study of Some Biological and Ecological Aspects, Aquaculture and Breeding Methods of Oriental Prawn Macrobrachium nipponense (De Haan, 1849) In Basrah, Iraq

Author name: طارق حطاب ياسين المالكي
Supervisor name: مالك حسن علي | امنة علي هاشم
General topic: Agricultural sciences
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: تضمنت الدراسة الحالية انشاء مفقس للروبيان الشرقي Macrobrachium nipponense وتجربته عمليا على الاناث الحاملة للبيض والمصطادة من منفذ المسحب خلال شهري حزيران وتموز لسنة 2013 واجراء عملية التكاثر وتسجيل اربع مراحل تطور جنينية في البيض، وتراوحت مدة التطور الجنيني للبيض بين (20 - (30 يوما.بينت الدراسة تسجيل افضل معدل وزن لليافعات في اثناء تربيتها في الاحواض المختبرية والمغذاة على عليقة ذات محتوى بروتيني 42 % مع نسب البقاء مرتفعة بين 90) - (95%، وهذا ما انعكس على التركيب الكيميائي لجسم الروبيان بعد التجربة. بينت نتائج السلوك بان الروبيان المدروس يفضل التعلق ضمن عمود الماء، اذ بلغ افضل معدل وزن عند استعمال عليقة الرقائق (Aquafin) ونسب بقاء 95 % خلال مدة التجربة، واظهرت نتائج تجارب الاقلمة الملحية واستهلاك الاوكسجين بان التركيز الملحي نصف القاتل (LC50) لليافعات خلال 96 ساعة كان 15.5 psu مع نسب بقاء مرتفعة في الملوحتين (0.9 و(5.0 psu، ولوحظ ارتفاع تدريجي باستهلاك الاوكسجين مع ارتفاع كل من درجة حرارة الماء والملوحة. بلغت نسبة الجنس 1 : 3.8 ذكور : اناث على التوالي. تراوح عدد البيض للروبيان المصطاد من منفذ المسحب بين 3,600) - (10,200 بيضة /انثى. في حين بلغ معدل التفقيس لليرقات (1632 ± (861 يرقة زؤيا، وكان معدل عدد اليرقات نسبة الى وزن الجسم 225 ± 110 /غم من وزن الجسم، كما لوحظ وجود ارتباط بين وزن الامهات مع كل من وزن البيض وعدد اليرقات الفاقسة، واظهرت نتائج رعاية اليرقات حديثة الفقس في انظمة الاستزراع المختلفة بان اغلب اوزانهواصل الى 3 ملغم خلال المدة الممتدة بين 12) - (22 يوما ، وكانت اعلى معدلات نسب بقاء لليرقات 63.75 % سجلت في الحوض الطيني فيما تراوحت بين 27.50) - (47.50% في الانظمة الاخرى.بلغت معدلات الاوزان النهائية (813 و978) ملغم لليافعات الفاقسة المرباة في الاقفاص الطافية والغاطسة على التوالي، في حين بلغت معدلات الاوزان 805) و950) ملغم لليافعات الطبيعية المرباة في الاقفاص الطافية والغاطسة على التوالي خلال 28 يوما، وبنسب بقاء بين (86 - 93)%، في حين تراوحت معدلات اوزان اليافعات المستزرعة في النظام المغلق من (938 والى 4010) ملغم خلال 90 يوما، وبنسب بقاء بين 86) - (92 %، وسجلت اعلى نسب البقاء ليرقات الروبيان ويافعاتها المرباة خلال 42 يوموابلغت 78.13% في الحوض الطيني فيما تراوحت بين (47.50 - (58.13% في الانظمة الاخرى. تحقق افضل معدل اوزان لليرقات المغذاة على عليقة (46% بروتين) ولمدة ستة اسابيع في الحوض الطيني وكان697 ملغم واقلها في الحاضنات وبلغ 312 ملغم، تتحول اليرقات حديثة الفقس (طور الزؤيا) الى طور ما بعد اليرقي بعد عمر 12) - (22 يوما، في حين طور اليرقة المتقدمة بعد عمر 23) - (44 يوما يتحول الى الطور اليافع الذي بدوره يتحول بعد عمر (45 - 129) يوما الى الطور قبل البالغ الذي بعد عمر 130 - 179 يوما يصل الى الطور البالغ، اي بعد عمر 180 يوما الروبيان يصبح في الطور البالغ.حددت انتاجية المفقس من الاحواض الداخلية بين (53,504 - (190,000 يرقة زؤيا، في حين ينتج بين (32,355 - (99,560 يرقة متقدمة، وتراوح الانتاج من الاحواض الخارجية ما بين (4,347 - (24,098 يرقة زؤيا، وبين 2,527) - (18,828 يرقة متقدمة، كذلك على ضوء نتائج المساحة الكلية للحوض الطيني وحوض الالياف الزجاجية فان عدد يرقات الزؤيا التي يمكن ان تستوعبها تتراوح ما بين 36,559) - (15,300,000 يرقة زؤيا، وبين (21,248 - 11,954,400) يرقة متقدمة، اما مجموع الانتاج الكلي في جميع انظمة الاستزراع الداخلية والخارجية فكان ( 12,600,188 - 15,760,462) يرقة زؤيا، وبين 9,753,349) - (12,197,630 يرقة متقدمة.بلغت النسب المئوية للتركيب الكيميائي لبعض مكونات العليقة المصنعة في الدراسة الحالية والمستعملة في تغذية اليرقات 44.84) و51.27 و2.57) % للبروتين والدهن والرماد على التوالي، في حين بلغت لعليقة مسحوق الاسماك المستعمل في تغذية الامهات واليافعات (40.37 و25.29 و15.13) % للبروتين والدهن والرماد على التوالي. | This study included hatchery construction for oriental prawn Macrobrachium nipponense, a gravid females were collected from Al - Masshab Outlet, Basrah, during the peak of occurrence in June - July / 2013, is for application of breeding process, with recorded four developmental of eggs, with range time of embryonic development stage of eggs between 20 - 30 day. The present study revealed that the best weight for juvenile in laboratory which were feeding on diet contain 42% protein with high survival rates between 90 - 95%, this reverse on the chemical composition of prawn body after the experiments. Showed result behaviour that this prawn preferred to feeing hold on the ponds columns, it reach a best weight by using flake feed (Aquafin), with survival rate 95% during the experimental period. The salinity acclimation and oxygen consumption showed that LC50 for juvenile was 15.5 psu after 96 hours, with high survival rate in tow salinities 0.9 and 5.0 psu. A gradual increasing in oxygen consumption with increasing temperature and salinity clearly proved in this experiment. Sexual ratio was recorded to be 1 : 3.8 for males : females respectively. The number of eggs were collected from Al - Masshab Outlet between 3,600 - 10,200 egg for number of females, while the ranged of all hatching larvae were 1,632 ± 861 zoae. The range of ratio of larvae to the ratio of body weight was 225 ± 110 g from body weight of prawn, and the present study showed that there is a correlation between female weight with both egg weight and number of hatching larvae, by using different nursing systems of newly hatched larvae for 12 to 22 day, and the larvae reached weight to 3 mg., while higher range of survival larvae were 63.75% in muddy pond, in others systems were 27.50 - 47.50 %.The final weight range were reached 813 and 978 (mg) for hatched juveniles that were reared in floating and emerged cages respectively, while the naturally reared Juvenile have weight range between 805, 950 (mg) for nature floating and emerged cages during 28 day, with a survival rate between 86 - 93%, and weight range of juvenile reared in closed system from 938 to 4,010 (mg) during 90 day, with survival rates 86 - 92 (%). High levels of survival rate recorded to be 78.13% for larvae and juvenile of prawn cultured earth in ground pond during 42 day, comparison with 47.50 - 58.13% in others system. The best weight for larvae that feeder on protein diet (46%) for six weeks in ground pond reached to 697 mg while the lowest weight in nursing system reached to 312 mg. The age which zoea needs Metamorphosis zoae between 12 - 22 days to reach post - larvae, were 23 - 44 day for Metamorphosis post - larvae to Juvenile, and this needs between 45 - 129 day for reached to sub - adult with in 130 - 179 day. After 180 day more prawn reached to adult stage.The hatchery production of zoea larvae was fluctuated between 53,504 - 190,000, it and between 32,355 - 99,560 post - larvae. The total production from earth pond (mud pond and fiberglass tank), the number of zoae larvae between 36,559 - 15,300,000 larvae, and between 21,248 - 11,954,400 post - larvae. While the total production in all culture systems were 12,600,188 - 15,760,462 zoea larvae, and between 9,753,349 - 12,197,630 post - larvae.The chemical composition for prepared diet the present study which used for nutritional diet experiment were 44.84 % protein, 51.27 % fat and 2.57 % ash. Fish meal constituents was 40.37 % protein, 25.29 % fat and15.13 % ash

دراسة لمرض لفحة سعف النخيل المتسبب عن الفطر Diplodia phoenicum وبعض الفطريات المرافقة له وامكانية مكافحة المرض كيميائيا واحيائيا == A study of date palm leaflets blight caused by Diplodia phoenicum and some fungi associated with it and the ability of it?s chemical and biological control

Author name: ضرغام صباح لفتة باقر العوفي
Supervisor name: محمد عامر فياض | يحيى عاشور صالح
General topic: Agricultural sciences
Specific topic: Plant - Prevention
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was carried out at the laboratories of plant protection Department - College of Agriculture - Basrah university for a.peroid during 1/9/2014 - 27/7/2015 . The study aimed to detect the leaflet bligh disease for different datepalm cultivars such as Chipchap ,Sayer , Zehdi and Khodrawi at three regoins, Medainah , Abu Al - khaseeb and Nashwa.The result showed that the infection percent was 86.51, 83.61 and 76.03% at Medainah , Abu Al - khaseeb and Nashwa respectively . The results also showed that the infection intensity was 25.96 , 23.56 and 21.1 % for the three above regoins respectively .The higher infection intensity was noticed on Chipchap culture which was 30% while the lowest one found on Sayer culture which was 23.3% .The infection intensity also differs according to surfaces , it was 76.37 % on the lower surface , while it was 55.55 , 37.91 and 24.34% on the middle ,foot and terminal of leaflet .Leaflet dried and oval pustules which contain the spores of serenomyces phoenicis were recorded as symptoms represented the scorch disease while the other fungi caused yellowish and spotting symptoms sixteen species belonging to 13 genera were isolated from the leaflets , these species were as follows : Chaetomium atrobrunneum , C.globosum , Chalaropsis thielavioides , C.radicicola ,Stemphylium.sp.,Bipolaris australis ,Cladosporium herbarum , Nigrospora sphaerica , Diplodia phoenicis , Phoma glomerata , Fusarium verticillioides , Alternaria alternate ,bA. longipes ,Coniochaete sacchareo , Melanospora sp., andS.phoenicis.Most of of them caused blight disease .Among of them D. phoenicis and S. phoenicis gave scorch symptoms pathogenicity testing .The study epplained that Extract agare was the best medium for A. phoenicis growth ;it was 7 cm , while PCA gave the lowest growth, the fungus growth , the best growth 6.83 cm was noticed on 25 c while the growth stopped on 35 c . PH also effected on the fungus radial growth , it was 4.35 cm at PH4 , while it was 6.57 cm at PH 8 .Tested fungicides revealed significant differences . Dazim and Revus Top inhibited the radial growth in a percent of 100% ,while it reduced to 83.34% with the fungicides Hunter , so Dithane M4S inhibited the radial growth to 54.22% . The biological agent Trichoderma harzianum antagonists with D. phoenicis in the alevel 1,P. fluorescence also inhibited D. phoenicis in a percent OF 100%.The field experiment revealed that pa fluorescence reduced infection intensity to 50% , while Dazim biological agent T. harzianum treatment were the lowest treatment in reducing the infection intensity which reduced to 4.1 and 6.3 respectively .

تاثير حقن بيض التفقيس بفيتامين A وE وD3 وخليطهم في بعض الصفات الانتاجية والفسلجية والسلوكية لطائر السمان الياباني (Coturnix japonica) == Effect Of In Ovo Injection of Vitamin A , E , D3 and AD3E on some Productive , Physiological and Behavioral Traits of Japanese Quail )Coturnix japonica)

Author name: صلاح مهدي كاطع سلمان
Supervisor name: طارق فرج شوكت | قصي موسى جعفر
General topic: Agricultural sciences
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in the Animal Production field which belong to the Technical College - AL - Massaiab - AL - Furat AL - Awsat University which included two experiments the first was during 4/3/2014 until 22/3/2014, the second was during 22/3/2014 until 10/8/2014 and the to investigate the effect of injection of japanese quail eggs A,E,D3 and AD3E Vitamins on some productive , reproduction, physiological , behavioral and hematological traits .A total number of 720 eggs were used in the experiment ,those eggs were randomly distributed into six treatments , 120 egg in each treatment . The eggs were injected before the first day of incubation in the air sac by vitamins. Treatments were arranged as fallow : T1 : Negative control treatment .T2 : Positive control treatment , it included injection of 50 microliter of corn oil for each egg.T3 : It included injection of 50 microliter per egg by vitamin A (100 IU/egg).T4 : It included injection of 50 microliter per egg by vitamin E (15 IU/egg).T5 : : It included injection of 50 microliter per egg by vitamin D3 (100 IU/egg).T6 : : It included injection of 50 microliter per egg with the blend of AD3E vitamins by the same concentration per egg.The most important results recorded in the first experiment were high significant increased (P≤0.05) of hatchability of fertile egg and post chick weight but not effect significant on embryo mortalities during embryogenesis .The second experiment included rearing of the post hatched chicks of the first experiment in wooden dataries , chicks were all randomly allocated inSummarybto three replicates for each treatment (17 chick / replicate) to study productive , reproductive , physiological, behavioral , histological , biochemical and hematological traits for Japanese quail.Results illustrated the followings : 1 - A significant increase (P≤0.05) of productive traits in treatments of injection (body weight , weight gain , feed utilization , feed conversion ratio ) and find significant increase egg production , egg weights and mass as well as shell characteristics (egg shell weigh and thickness ) in Treatments (T5 , T6 ) comparisons with the negative and positive control treatments .2 - A significant effect (P≤0.05) on early maturation for males and females in treatments of vitamins injection , more over there were a significant increase of their body weights during maturation and early behavioral jumping with a significant surpass of cloaca area in comparison with males in control treatments.3 - A significant increase (P≤0.05) of relative weights of testes in males of all ages in injection treatments with a significant increase of seminal tubules diameters and thickness of germinal layer of testes during 28 and 47 days in comparison with that of the negative and positive controls.4 - A significant increase (P≤0.05) of relative weights of ovaries with an increase of the primary follicle diameter in females of injection treatments during 28 , 47 and 69 days in comparison with that of the control .5 - A significant increase (P≤0.05) of relative weight of fabricia gland and spleen for both sexes of birds in injection treatments , more over the interaction between vitamins injection and sex was significant in all ages.6 - A significant increase (P≤0.05) of PCV in injection treatment , moreover the PCV was increased in females at 28 and 47 days, the interaction between vitamins injection and sex was significant in all ages .7 - No significant increase (P≤0.05) was noticed in Hb of injection treatments at 28 but there were a significant increase (P<0.05) in 47 days in vitamins injection treatments and there was a significant increase (P≤0.05) of Hb in 69 days in the sixth treatment in comparison with the control treatments, and it was numerical on other treatments ( 3rd, 4th and 5th) there was no interaction between vitamins injection and sex at 28 days but the interaction was significant in at 47 and 69 days .8 - There were no significant increase (P≤0.05) of RBC in injection treatments at 28 days but it was significant at 47 days in the third and sixth treatments , at 69 days there was a significant increase of RBC in injection treatment as compared with the control treatments , the interaction between injection of vitamins with sex was not significant at 28 days but it was significant at 69 days .9 - There were no significant effect (P≤0.05) in WBC in vitamins injection at 28 , 47 , 69 days as compared with the control treatments moreover there was no significant interaction between injection treatment and sex.10 - There were significant increase (P≤0.05) in LH , FSH and testosterone levels in serum of vitamins injection treatment for both sexes , the interaction between treatment sex was significant in all ages .11 - There were a significant surpass (P≤0.05) of total protein level in injection treatments at all ages as compared with the control treatments and the interaction between treatments and sex was significant at all ages . glucose and cholesterol level were not affected according to the treatment but females were significant surpasses on males at all ages . The interaction between treatment and sex was not significant at all ages12 - There were a significant surpass (P≤0.05) of Ca and P in injection treatment at all ages as compared with control treatments , the interaction between treatment and sex for Ca and P level was significant at all ages .13 - The study revealed no significant improvement in concentration Ca , P and ash in tibia bone , weight and length of thigh for both sexes in injection treatment at all ages .

تاثير انظمة الاضاءة والتقنين الغذائي الزمني في بعض الصفات الانتاجية والفسلجية والتناسلية لطائر السمان الياباني (Coturnix japonica) == The Effect of Lighting Systems and Feed Restriction timing on Some Productive, Physiological and Reproductive Traits of Japanese Quail (Coturnix japonica)

Author name: صباح كاظم مرزوق الحمود
Supervisor name: ریاض كاظم موسى | ربیعة جدوع عباس
General topic: Agricultural sciences
Specific topic: Zoology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

تصميم وتصنيع وتقييم الاداء الميكانيكي لالة زراعية من خلال وضع السماد العضوي في خنادق تحت سطح التربة وتاثيرها في بعض خصائصها ونمو وحاصل الذرة الصفراء Zea mays L == Designing , constructing and evaluating the mechanical performance for agricultural machine laying the manure under the soil surface and its effect on some of soil properties and growth and yield of corn ( Zea mays L.

Author name: صادق جبار محسن
Supervisor name: شاكر حنتوش عداي | محمد عبد الله عبد الكريم
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: ditch opener and manure laying machine was designed and manufactured in the agricultural machines and equipment dept., Agriculture college, Basrah university in 2015. This machine was evaluated to determine its mechanical performance and its ability in laying manure in ditches under the soil surface as well as the effect of the added manure on the soil physical and chemical properties, corn crop growth and production parameters.The machine consists of a frame, manure tank, chemical fertilizers tank, subsoiler for soil disturbance down to 80 cm, two boards for soil digging out to open the ditch , two boards to return the soil to ditch to bury the manure , manure and chemical fertilizers feeding mechanisms and blades for manure mixing with the soil. Also its provide with two pipes to transfer the chemical fertilizers to the soil and a petrol engine to operate the two feeding mechanism.The machine disturbed the soil by the subsoiler and digging different ditches of different depths by two boards . In additional to that it can lay the manure at different levels in the opened ditches using the feeding mechanism and thereafter buried the manure in the soil . The manure can be laid under the soil as separate layer or mixed with soil using the mixing blades.Three experiments were conducted to evaluate the machine performance. These experiments are : (1) - Laboratory experiments were conducted to study the effect of the machine forward speeds (0.32, 0.46 and 0.63 m sec - 1), manure feeding mechanisms rotating speeds (360, 440, 560, 710 and 880 rpm) and two manure feeding openings areas (0.024 and 0.048 m2) as well as the interaction of the above parameters on the amount of the manure per hectare (ton ha - 1). These results were analyzed using CRD and the statistical program (Genstat). Mean results were compared using least significant difference (RLSD) at 0.05 level.The results showed the manure amount per hectare significantly increased as the rotating speeds of the feeding mechanism increased,Bthe forward speeds decreased and the feeding opening areas increased. The highest value was recorded for forward speed of 0.32 m sec - 1 , rotating speed of 880 rpm and feeding open area of 0.048 m2. These means are 64.17, 62.06 and 54.06 ton ha - 1 respectively. while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and the feeding open area of 0.024 m2 gave the lower values which they are 26.91, 30.04 and 33.75 ton ha - 1 respectively. Also, the forward speed of 0.32 m sec - 1 , the rotating speed of 880 rpm and the feeding area of 0.048 m2 recorded the highest amount of manure per hectare (110.00 ton) while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and manure feeding area of 0.024 m2 recorded the lowest value of manure per hectare (4.00 ton).(2) - A field experiment was conducted in silty loam soil in the agriculture college field at Garmit Ali location to study the effect of the implement operation depths (10, 20, 30, 40 and 50 cm), the angle between the soil digging out boards (45⁰ and 60⁰) two soil types namely cultivated soil before one year and uncultivated soil and their interaction on the implement mechanical performance parameters . These parameters are the draft force, soil disturbed area and the energy utilization efficiency (EUE) as well as the draft force requirement of the implement units (subsoiler, ditch opening boards, soil returning boards and soil mixing blades). The results were analyzed using RCBD. The analyzing was carried out by the statistical program (Genstat). Mean results were compared using RLSD at probability level of 5% , and showed the followings : (2 - 1) - The draft force, the disturbed area and the energy utilization efficiency increased significantly when the operating depth increased from 10 to 50 cm. The amount of the increase is 26.66 kN , 0.4111 m2 and 7.67 m3 mJ - 1 respectively.(2 - 2) - The angle between the opening boards of 60⁰ significantly surpassed angle of 45⁰ in increasing the draft force, the disturbed area and EUE. The percentage of increase is 12.55 , 21.93 and 10.85 % respectively.C(2 - 3) - The draft force decreased while the disturbed area and EUE increased by 16.30, 14.91 and 20.72 % in the disturbed soil compared with undisturbed soil respectively.(2 - 4) - The draft force requirement of the subsoiler was higher than that for the other implement parts. The share of the draft force requirement of the subsoiler was 60 % out of the total draft force requirement of the implement while it was 26, 9 and 5 % for the ditch opening boards, the soil returning boards and soil mixing blades respectively.(2 - 5) - The draft force requirement of all implement units increased significantly with implement operating depths and the angle between the ditch opening boards and it was higher in the uncultivated soil compared with the cultivated soil. The draft force requirement of the soil returning boards and the soil mixing blades in both soil types was not significantly different.(3) - Another field experiment was conducted in the field of the agriculture college at Garmit Ali location in the season of 2015 in a cultivated soil of silty loam texture. The aims of the experiment were to study the effect of the added manure level (0, 20 and 40 ton ha - 1), the depths of added manure (10, 20 and 30 cm) and the methods of the addition (subsoil layer and mixing with soil) and their interaction on the soil properties and corn crop (Zea mays L.) growth parameters and yield at the end of the growth season.The soil was plowed and harrowed (soil clods breaking down). The manure was added thereafter by the implement in the soil according to the studied treatments. The corn seeds were sown in rows at 1/7/2015. The drip irrigation method was used to irrigate plants until the end of the experiment 10/10/2015. The other agricultural operations were conducted according to methods used in the around area.Soil and plants samples were collected for analysis and the corn seeds yield was calculated at the end of the growth season. The measurements included soil bulk density, soil moisture content, soilDsalinity, soil pH, the dry weight of the shoot, N, P, K uptake in shoot and the total seeds production.The split - split plot in RCBD design was used to analyze the means using Genstst program. Mean results were compared using RLSD at probability level of 5 %, and showed the followings : (3 - 1) - The manure level of 40 ton ha - 1 surpassed the other two levels (0 and 20 ton ha - 1) in giving higher values of soil moisture content, higher uptake of N, P, K, a greater amount of dry weight and total yield while it gave lower soil bulk density, soil salinity and soil pH.(3 - 2) - Increasing the depth of added manure from 10 to 30 cm with the mixing method lead to significant increase in the soil moisture content, uptake of N, P, K, the dry weight and the total yield production. Whereas the soil bulk density, soil salinity significantly reduced while the soil pH was not significantly affected.(3 - 3) - Mixing of 40 ton manure ha - 1 at depth of 30 cm gave the highest crop growth and yield production which they are 13.94 and 6.75 ton ha - 1, respectively.

عزل وتشخيص بكتريا Bacillus cereus واستعمالها في انتاج متعدد هيدروكسي البيوترات == Isolation and Identification of Bacillus cereus Bacteria and Using in Polyhydroxybutyrate Productio

Author name: شيماء ذياب جدوع السهلاني
Supervisor name: امال كاظم غضبان الاسدي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Forty three local bacterial isolates were obtained after heating treatment for different sources collected from many places in Basra government. Included : Vegetables, Fruit, Pastry (cakes), Legumes, Soils, Sand, Animal manure, the papyrus plant, milk, water liquefaction and sewage water. Primary screening for the isolates by Sudan black B dye only. 22 isolates were chosen with strong staining, and the identification of all the isolates revealed were belong to genus Bacillus by studying microscopic and biochemical tests. They were : 4 isolates Bacillus cereus, 2 isolates Bacillus firmus, 2 isolates Bacillus lichenformis , 3 isolates Bacillus megaterium, 4 isolates Bacillus mycoides , 3 isolates Bacillus pumilus and 4 isolates Bacillus subtilis.Secondary screening of these isolates was Polyhydroxybutyrate (PHB) produced, Bacillus cereus B5 given 2.4 g / L the highest production of PHB. A PCR technique was used for 16S rRNA test and detecting the gene of PHB production in Bacillus cereus B5.The highest PHB production from Bacillus cereus B5 was 6.2 g / L, biomass 8.4 g / L and yield 73.8% the by using optimum conditions : incubation temperature 35̊ C, for 48 hours aerobically by using shaking incubator for 150 RPM/ min, 2% inoculum volume, the pH was adjusted to 7, and the production media where contained 1% glucose as carbon source and peptone as a nitrogen source. 3% have dated juice was the best substitute for glucose as carbon source, it gave 7.11 g PHB / L and the yield 79 %.Analysis with FT - IR was showed, that PHB produced from date juice media had a peak at 1723 cm - 1 this means it belong to an esterpolymers group. And GC - MS showed 12 compounds produce from analyzing PHB as short chains of fatty acids.The properties of PHB produced from date juice media were : The degradation temperature was 312̊ C, the percentage of crystallization was 60%, molecular weight was 423.674 KDa., permeability of water vapor was 56.2 ×10 - 8 g / Pa.s.m2, tensile strength was 24.9 MPa, the blend 40% polyethyleneglycol as plasticized with PHB increased the percentage of elongation to 8.7%. PHB is non - toxic and without hemolysis on human blood.Studying biodegradation of the plastic films of PHB by using bacterial isolates were E. coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus in petri dish showed that all isolates can degrade PHB, biodegradation in soil and at soil surface was 100% after 28 days.PHB packages increased the shelf life of strawberries and grapes after storage for 15 days, compared with polyethylene packages, and It was reduced the numbers of microorganisms in butter, the percentage of free fatty acids and peroxide value compared with butter with polyethylene after 15 days

استجابة زهرة الشمس للحامض الاميني البولين تحت مدد ري مختلفة == The Response of Sunflower (Helianthus annuus.L) to Proline under different Irrigation intervals

Author name: شاكر اسماعيل عبد الرضا
Supervisor name: هاشم رشيد مجيد
General topic: Agricultural sciences
Specific topic: Plant - Field Crops
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: Field experiment was conducted during the fall season of year 2014 done in farmer's fields in the Auffia area southwest of Missan province (about 14 km .1 - m the soil was clay loam with 4.2 ds . city center)fromThe objectives were to determent the response of Sunflower (Helianthus annuus .L) hybrid Flame to amino acid - Proline in order to reduce water stress. The experiment executed in split - plot (R.C.B.D) design with three replicates. The experiment were three levels of proline (P1 spray Proline concentration in addition to the control 1 - ; P2 spray Proline concentration 100 mg L 1 - 50 mg LP0 as distilled water were located in sub - plot) while the other factor was irrigation period (W1= irrigation every 10 days; W2= irrigation every 15 days andW3= irrigation every 20 days) as the main - plot.The crop sprayed with Proline acid after mixed with water in two phases of growth is V6 (the phase of six real leaves) and R1 (flowering stage) with application of irrigation system. After the plants reaches to physiologic maturity and growth characteristics measured; the contains of yield and quality characteristics and the results were analyses statistically and compared with averages LSD test. The results showed the following.e uhigher valgave to the control compared 1 - 100 mg Lwith praying ProlineS - 1for growth measurement in mean of plant height; number of leaves; stalk diameter ; head diameter and LAI and they were (126. 19 cm ; 28. 60 leave ; 23. 27 mm; 17. 48 cm and 2. 28) respectively. Also increased the mean of yield and seed yield; weight of 100 seed the; 1 - head er of seedsnumb components) 1 - 16 ton h .and 1 1 - 48 tons h .0 4 g; 3 .40 seeds; 6 .(1003 oil yield ofrespectively.2 - Applying irrigation every 10 days improve all growth characteristics; yield's components and quality compared to applying irrigation every 20 days, which gave the lowest mean in above characters.3 - The results also showed that the interaction of applying Proline and irrigation periods was significant for the most of the growth characteristics; yield and quality characteristics. It was concluded from to reduced period of irrigation not differ from hich W .1 - 100 mg L Proline once applying daysevery 15 applying irrigation every 10 days for most of the studied characteristics. Is the most important yield; oil production and this can saves 25% of irrigation water under conditions - Missan Auffia area. That reduces the wastae of water resources or expansion of the agricultural area and increase the yield.

اســتـخـلاص السـكريات المـتــعددة مـــــن الطــحــلــب الاخضر Cladophora sp. وتوصيفها واســــتعمالها في اقراص اللحم البقري == Extraction and Characterization of Green Algae Cladophora sp. Polysaccharide and use it in Beef Patties

Author name: سـهام ولـيـد عـلك الامارة
Supervisor name: ام البشر حميد جابر الموسوي | روضة محمود العلي
General topic: Agricultural sciences
Specific topic: Food
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: تضمنت الدراسة استخلاص السكريات المتعددة من الطحالب، اذ تــــــــــــــم جــــــــــــمع عـــــــــــــينات مـــــــــــــن الطحالب الخضراء من مياه شط العرب ضمن منطقة كرمة علي في البصرة وبعد التنظيف والتنقية والتجفيف تم تشخيصهواتبين انها تعود الى جنسCladophora sp. ، درس تركيبها الكيميائي على اساس الوزن الجاف من الرطوبة والرماد والبروتين والدهن والكاربوهيدرات اذ بلغت نسبة الرطوبة 4.16% والرماد 29.78% والبروتين 16.10% والدهن %1.25 والكاربوهيدرات %48.71. وتم استخلاص السكريات المتعددة من الطحالب بواسطة كاربونات الصوديوم Na2CO3، وقدر محتواها من السكريات الكلية اذ بلغت 78.4%. كذلك درس تاثير ظروف الاستخلاص على حاصل السكريات المتعددة شملت نسبة خلط المادة الاولية : المذيب (غم/ مل) اذ استعملت اربع نسب 7 : 1و9 : 1 و1 : 12 و15 : 1 حيث اعطت نسبة 9 : 1 اعلى حاصل واقل نسبة حاصل كانت %2.3بنسبة خلط 15 : 1. استعملت درجات حرارة (60 و80 و100) م حيث اعطت درجة حرارة 80 م اعلى نسبة حاصل %7.53، اما اقل نسبة حاصل فكان عند درجـــــــــــــــــة حرارة 100 م %2.09. درس تاثير مدة الاستخلاص واستعملت مدد زمنية (2 و4 و6) ساعة اذ اعطت مدة 2و4 ساعة نسبة حاصل 6.95 و%7.60، بينما اعطت مدة استخلاص 6 ساعات نسبة حاصل%3.40 وهي الاقل، ودرس تاثير الرقم الهيدروجيني حيث اعطى الرقــــــــم الـــــــــــــهيدروجيني 2 اعلى حاصل وهو 7.49مقارنة مع 4 و6 حيث كانت نسبتي الحاصل 2.33 و1.92% على التوالي. من خلال دراسة الظروف اعلاه تبين النتائج ان افضل نسبة حاصل تم الحصول عليها عند الاستخلاص بكاربونات الصوديوم بنسبة خلط 9 : 1 وبدرجة حرارة 80 م لمدة 4 ساعات وعند رقم هيدروجيني2. درست الخصائص الفيزيوكيميائية للسكريات المتعددة المستخلصة من الطحالب وكانت النتائج كالاتي : - 1 - اظهرت نتائج اللزوجة النسبية للسكريات المتعددة المستخلصة من الطحالب ان اللزوجة تزداد بزيادة التركيز وتقل بزيادة درجة الحرارة حيث كانت اعلى قيمة للزوجة بدرجة حرارة 30 م (11.0020) واقل قيمة للزوجة النسبية (8.0576) عند درجة حرارة 50 م وكانت لزوجة السكريات المتعددة المستخلصة اقل من لزوجة الجينات الصوديوم القياسية.2 - لوحظ ان قابلية السكريات المتعددة المستخلصة لامتصاص الماء وربط الدهن تزداد بزيادة التركيز وكانت مقاربة لقابلية الجينات الصوديوم القياسية. - كانت النسبة المئوية لذوبان السكريات المتعددة المستخلصة 69.72% وهي اعلى من ذوبان الجينات الصوديوم القياسية والبالغة 65.68%. 4 - اظهرت النتائج انخفاض قابلية السكريات المتعددة على تكوين الرغوة.5 - قدر الوزن الجزيئي للسكريات المتعددة المستخلصة بقياس لزوجة السكريات المتعددة المستخلصة من الطحالب اذ تم تقدير اللزوجة الحقيقية لاستخراج الوزن الجزيئي الذي بلغ 875.26 كيلو دالتون. تم دراسة التحلل الوزني الحراري للسكريات المتعددة المستخلصة وكان الفقدان بحدود7% والمتبقي93% عند درجة حرارة 515.27 م.6 - اختبرت السمية الخلوية للسكريات المتعددة المستخلصة من الطحالب اتجاه كريات الدم الحمر للانسان واظهرت النتائج عدم سميتها لكافة التراكيز المستعملة ولمدد الحضن 10 و30 و60 دقيقة.7 - تم ادخال السكريات المتعددة المستخلصة والجينات الصوديوم القياسية في اقراص اللحم البقري وبنسب 0.2 و0.4 و0.6% وخزن بالتبريد بدرجة حرارة 4 م ولمدد زمنية1 و4 و7 يوم.8 - درست الصفات الفيزيائية لاقراص اللحم البقري اظهرت النتائج ارتفاعا معنويا p<0.05)) في قـــــــيم قابلية حمل الماء لاقراص اللــحم المعاملة بالــــــــــــــسكريات الـــــــــــــــــمتعددة (المعاملة المحضرة) والجينات الصوديوم (المعاملة القياسية) مقارنة بالعينة الضابطة. بينت النتائج ان الرقم الهيدروجيني في اقراص اللحم يزداد معنويا بتقدم مدة الحفظ بالتبريد ولوحظ انخفاض نسبة الفقد في الوزن اثناء الطبخ وارتفاع نسبة حاصل الطبخ في اقراص اللحم البقري المعاملة بالسكريات المتعددة والجينات الصوديوم القياسية مقارنة بالعينة الضابطة. 9 - درست الصفات الحسية لاقراص اللحم والتي شملت اللون والنكهة والطراوة والعصيرية والقبول العام وقد اظهرت بعضها اختلافا معنويا((p<0.05 خلال مدة الحفظ بالتبريد ولم يظهر البعض الاخر اختلافا معنويا فالنسبة لدرجات تقييم اللون لاقراص اللحم المفروم لم تتاثر معنويا عند معاملة اقراص اللحم بالسكريات المتعددة المحضرة والجينات الصوديوم القياسية، اما درجات تـقييم صفـــــــــــــــــة النـــــــــــكهة لاقراص اللحــــــــــم فقد تاثرت معنويا p<0.05)) عند المعاملة بالسكريات المتعددة المحضرة والجينات الصوديوم القياسية مقارنة بالعينة الضابطة. | The study included extraction of polysaccharides from algae. The green algae was collected from Shatt Alarab water in Karmat Ali in Basrah, the green algae was purified and it is Cladophora sp. Polysaccharides was extracted by sodium carbonate Na2CO3. It̓s chemical composition including moisture, ash, protein, fat , carbohydrate and total saccharides was studied and it was moisture 4.16%, ash 29.78%, protein 16.10% at 1.25%, carbohydrate 48.71% and total sharrides 78.4%. The effect of extract conditions on polysaccharides yield were also studied included mixing percentage 1 : 7, 1 : 9, 1 : 12 and 1 : 15 and 1 : 9 exhibited the highest yield (7.48%) and the lowest yield (3.2%) when we use 1 : 15. The extraction was carried on different temperature 60°C, 80°C and100°C the highest yield was 7.53% when extraction on 80°C .The effect of the time of extraction in 2 hrs, 4hrs so the yield was 6.95% and 7.60% for 2 and 4 hrs respectively, the highest yield was 7.49% when extraction on pH 2. The results showed that the best yield was when the extraction on 1 : 9 and 80 °C for 4hrs and pH 2. The physiochemical properties for polysaccharides wer studied and the result showed the relative viscosity was increased with the increase of concentration and decrease with the increase of temperature the highest viscosity was 11.0020 in 30°C and the lowest was 8.0576 in 50 °C. The ability of polysaccharides for water absorbtion and fat binding was noticed that it was increased with the increased of concentration, the percentage of polysaccharides solubility was 69.72%, and it was higher than sodium alginate (65.68%). The results also showed decrease of foaming of polysaccharides because of it̕s high viscosity. The molecular weight of polysaccharides was 875.26 Kdal, the Thermo Gravimetric Analysis (TGA) was 7% for loss and the residue was 93%. The test of cytotoxicity was appaired that the polysaccharides was no toxic in all concentration and for all incubation periods 10, 30 and 60 min. The extracted polysaccharides and sodium alginate were used in beef patties for 0.2%, 0.4% and 0.6% and storage for 1, 4 and 7 days, the results showed that there were significant increased (p<0.05) in water holding capacity in all treatments comparing with control and pH increased during storage periode and also noticed that cooking loss was decreased and increased in cooking yield for beef patties treated with polysaccharides and sodium alginate comparing with control. The results showed that addition of extracted polysaccharides and sodium alginate to beef patties improved sensory properties (color, falvor, tenderness, juicness and overal acceptability) during storage periode compared with control.

تاثير مستويات مختلفة من السماد الفوسفاتي في نمو وحاصل ثلاثة اصناف من محصول الحنطة Triticum aestivum L == Effect of different levels of Phosphate fertilizaer on growth and yield of three cultivars of Wheat (Triticum aestivum L.)

Author name: سندس كامل جبار الحلفي
Supervisor name: هاشم رشيد مجيد
General topic: Agricultural sciences
Specific topic: Plant - Field Crops
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: نفذت تجربة حقلية خلال الموسم الشتوي 2013 / 2014 في حقول احد المزارعين في منطقة الشنانة التابعة لقضاء القرنة ( 65 كم شمال مركز محافظة البصرة ) في تربة مزيجية طينية بهدف دراسة استجابة ثلاثة اصناف من الحنطة الناعمة . Triticum aestivum L (اللطيفية وابوغريب - 3 وتموز - 2) في النمو والحاصل والنوعية لتاثير اضافة اربعة مستويات من السماد الفوسفاتي ( 0 و40 و80 و120 كغم P2O5 ه - 1 ) طبقت التجربة باسلوب التجارب العاملية ووزعت المعاملات وفقا لتصميم القطاعات العشوائية الكاملة بثلاثة مكررات . اظهرت نتائج الدراسة ان الاصناف اختلفت معنويا فيما بينها في معظم الصفات المدروسة اذ تفوق الصنف اللطيفية في صفة ارتفاع النبات ( 96,44 سم) ومساحة ورقة العلم (32,97 ســم2) وطول السنبلة (10,41سم) وعدد الحبوب في السنبلة (61,50 حبة سنبلة - ١) السنبلة ونسبة البروتين (11,84 ٪) في حين تفوق الصنف ابوغريب - 3 في صفة عدد الاشطاء(600,35 شطا م - 1) وعدد السنابل (572,07 سنبلة م - 1) ووزن 1000 حبة ( 31,09 غم ) وحاصــل الحبوب ( 6,31طن هــ - ١ ) والحاصل الحيوي( 14,64 طن هــ - ١ ). واشارت النتائج الى تفوق المستوى السمادي 120كغم P2O5 هـ - ١ معنويا في صفات ارتفاع النبات (99,11ســم ) ومساحة ورقة العلم ( 35,74 ســم2 ) وطول السنبلة (10,68 ســم) وعـــدد الاشطاء الكلي(621,82 شطا م - 1) وفي جميع صفات الحاصل وحاصل الحبوب( 6,68 طن هــ - ١ ) والحاصل الحيوي (14,98 طن ه - 1) ودليل الحصاد(44,58 ٪) ونسبة البروتين في الحبوب (12,62 ٪) . كان التداخل معنويا بين الاصناف ومستويات الفسفور في حاصل الحبوب وحققت التوليفة للصنف ابو غريب - 3 اعلى متوسط لحاصل الحبوب (6,98 طن هــ - ١) ويلاحظ من النتائج ان زيادة حاصل ابو غريب - 3 عند المستوى 120 كغم P2O5 هـ - ١ هو نتيجة لزيادة عدد الاشطاء (669,33 شطا م - 1 ) وزيادة مكونات الحاصل وهي عدد السنابل(636,81 سنبلة م - 1) ووزن 1000 حبة(33,03 غم) . | A field experiment was conducted during winter season of 2013 - 2014 at AL - shinana that belong to AL - Qurna about ( 65) Km north Basrha in silty loam soil in order to determine the best production of the three cultivars of wheat (Triticum aestivum L.) (Abu Graib - 3 , Tammoz - 2 and Latifia ) to determine the best level of phosphate fertilizer (0 , 40 , 80 , 120 kg P2O5 hˉ1 ) .The experiment design was R C B D with treatments arranged in factorial in three replicates . Results showed that cultivars are different in it's characteristics as the Latifia superior in plant height (96.44 cm ) flag leaf area ( 32.97 cm2 ), spike length ( 10.41 cm ) number of grain per spike( 61.50 ) and protein content ( 11.84% ). Abu Graib - 3 superior in number of tillers .mˉ2(600.35) number of spikes .mˉ2(572.07) weight of 1000 grain ( 31.09 gm) , grain yield (6.31 ton.h - 1 ) biological yield (14.64 ton.h - 1 ) Results showed a variance among fertilizer level where 120 kg P2O5 hˉ1 caused highest number of , plant height (99.11 cm ) flag leaf area (35.74 cm2) , spike length ( 10.68 cm), number of tillers (621.82 ) and all yield component and grain yield ( 6.68 ton .hˉ ¹), biological yield ( 14.58 ton .hˉ ¹) , protein (12.628% ) and harvesting index(44,58 %) . Results also showed a significant interaction between cultivars and P levels, grain yield which increased in all cultivars with increase in P levels grain yield obtained at treatment Abu - Graib - 3 ×120 kg P2O5 hˉ1 (6.98 ton.h - 1) the increase in grain yield Abu - Graib due to higher yield components number of spike m - 2 (636. 81) and 1000 - grain weight (33.03 gm

تاثير اضافة النتروجين في امتصاص NوPوK وتوزيعها في اجزاء النبات ونمو وحاصل ثلاثة اصناف من الحنطة Triticum aestivum L. == EFFECT OF NITROGEN APPLCATION ON N, P, K UPTAKE AND DISTRIBUTION WHIHIN PLANT PARTS, GROWTH AND YIELD OF THREE WHEAT CULTIVARS Triticum aestivum L.

Author name: سندس عبد الكريم محمد العبد الله
Supervisor name: عبد المهدي صالح حسين الانصاري | وليد عبد الرضا السباهي
General topic: Agricultural sciences
Specific topic: Food
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: A field experiment was conducted at Al - Daire site 40 km north of center Basrah Governorate .The study was conducted to investigate the effects of different nitrogen fertilizer levels on dry matter accumulation, (N, P, K) uptake, distribution in different parts of three cultivars of wheat plants and their yield during two seasons (2011 - 2012 & 2012 - 2013).The experiment was split - plots in R.C.B.D design with three replicates, Nitrogen rates (0, 60,120 and 240Kg ha - 1 occupied the main plots, while (IPA - 99, Abu - Graib and Latifia) Cultivars Occupied the sub - plots. Samples of wheat plants were collected at 10 growth stages : seedling stage, tillering (main shoot and 1 tiller), tillering (main shoot and 4 tillers), stem elongation, booting, 50% of spike emergence, 50% flowering, milk stage, dough stage and maturity. Each plant sample was separated into different plant parts : blades, sheathes, stems, flag leaves, spikes and seeds. These parts were dried at 70°C and dry weight of each part was recorded and sub sampled for chemical analysis of N , P , and K. Growth parameters (No. of day till 50% flowering , No. of day from 50% flowering to Maturity , flag leaf area , plant height, spike length and No of tillers m - 2) , yield components (No. of spike m - 2, No. of grain in spike and weight of 1000 grain ),grain yield, biological yield, harvest Index, grain protein percentage and nitrogen use efficiency were also studied.Results showed that dry matter accumulation in plants of all cultivars did not differ at early growth period, While after the growth stage of 50% flowering plants dry weight of Cv.IPA - 99 was higher than other cultivars, This difference in dry weight continue till maturity stage at which total dry weight were 14473.58, 13373.96 and 12743.59 Kg ha - 1 for IPA - 99, Abu - Graib - 3 and Latifia respectively.Dry matter yield were not affected by N levels during the early growth period ,then after dry weight of plants increased as N rates increased ,total dry weight were10752.61, 12737.49 and 15347.10 Kg ha - 1for 0, 60 , 120 Kg Nha - 1 respectively, with no differences between 120 and 240 Kg Nha - 1 levels.Dry matter of plant parts differed among cultivars, with IPA - 99 having the highest results. Increasing nitrogen rates increased weight of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.IIResults also showed reduces in dry weight of different plant parts(except grains)after reaching a maximum weigh at dough stage for Spikes, and 50% flowering for the other parts. The highest decreased was in blades and lowest in sheathes. The application of N fertilizer decreased translocation of dry matter from plant parts to grains. Data showed that no differences in nutrient uptake were observed for cultivars till ending of tillering stage, but after this stage IPA - 99 cultivar showed highest uptake till the end of the season. Nitrogen uptake was157.68,136.72,124.51 Kgha - 1 ,P uptake 19.70,16.93,15,67 Kg ha - 1, K uptake136.30,121.10,112,60 Kg ha - 1 for IPA - 99,Abu - Graib - 3 and Latifia, respectively.Increasing N rate increased nutrient uptake of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.uptake of N, P, and K for all cultivars and nitrogen rates declined as the season progressed, this decline differed among nutrients and plant parts.Grain contained 59.20, 79.00 and 7.45% of total nutrient N, P and K uptake, whereas vegetative parts contained 40.80, 21.00 and 92.55% of total nutrient uptake, respectively.Cultivar IPA - 99 resulted in highest grain yield among studied cultivars. Increasing N rate from 0 to 120 Kg N ha - 1 only increased grain yield, the rate 240 KgNha - 1 did not affect yield significantly as compared with that of 120 Kg N ha - 1.Results also showed a significant interaction between Cultivars and N rates. The highest grain yield obtained at treatment IPA - 99×240 Kg N ha - 1with no significant differences than IPA - 99×120 KgNha - 1.Results indicated that highest nitrogen use efficiency (66.23%) was found in plant of IPA - 99 cultivar, whereas, the lowest efficiency (47.37%) was found in plant of Abu - Graib - 3 cultivar. At all cultivars increasing N rates to 240 KgNha - 1 decreased nitrogen use efficiency.Therefore it can be concluded that 120 Kg Nha - 1 can be recommended as the best rate for N to all cultivars under similar growing conditions

معالجة مياه الصرف الصناعي لمعمل الاسمدة في المنطقة الجنوبية واعادة استعماها لري نبات الطماطة Lycopersicon esculentum Mill == Industrial wastewater treatment of fertilizer factory south region and reuse for tomato irrigation( Lycopersicon esculentum Mill.)

Author name: سماح رسول جويد العكيلي
Supervisor name: محمد عبد الله عبد الكريم | نجلة جبر محمد الاميري
General topic: Agricultural sciences
Specific topic: Soil and Water
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted to investigate the possibility of using the effluent originated from fertilizers factory/ south region, Basrah province for tomato irrigation. Effluent samples were collected from urea unite line, ammonia unite lion, and collection basin at nine periods during 3\9\2015 to 5\8\2016. Tap water was used as control water. Three types of filters were used to enhance the characteristics of water collected from the fertilizers factory which are Rice Husk Ash filter, sand filter and Rice Husk Ash + sand filter(75 : 25). chemical characteristics (EC, PH, Ca++, Mg++, Na+, K+, Cl - , SO4 - - , total hardness, NO3 - , NH4+, urea) were obtained before and after filtration then Removal efficiency (%) was calculated. Basin on above experiment, a pot culture experiment was conducted to study the effect of fertilizers industry effluents on chemical parameters of soil and its impact on growth parameters of the tomato plant. Each pot was prepared by filling loamy sand soil 10 kg collected from a farm nearby the factory. All the pots were fertilized by manure, phosphorus and potassium. Nitrogen fertilizer (urea) was applied an equivalent of 0, 50% and 100% of recommended level (300 kg N ha - 1). Tomato seedlings (hybride Newton F1) were transplanted in the pots. The pots were irrigated with above fertilizer industry effluents or tap water after the seedlings standing in the pots. After 90 days, soil and plant samples were collected to measure EC, pH, NO3 - in soil, NH4+ in soil, plant height, shoot dry weight, N concentration,number of flowers plant - 1 and number of fruits plant - 1.The results showed that there was a significant changes among sample periods in all water characteristics. The highest removal efficiency of urea, NH4+, NO3 - , cations and anions were recorded by using Rice Husk Ash filter, while the lowest efficiency were recorded by using sand filter. Data alsoBshowed that chemical parameters (EC, NH4+ and NO3 - ) of soil were differed by different filter used and follow the order : control (without filtration) < sand filter < Rice Husk Ash + sand filter < Rice Husk Ash filter. This result was reflected on plant growth parameters (plant high, shoot dry weight, N concentration ,number of flowers plant - 1 and number of fruits plant - 1) and follow the order : Rice Husk Ash + sand filter < Rice Husk Ash filter < sand filter < control. However soil pH was not affected at different filters used. Increasing the level of nitrogen significantly increased EC, NH4+ and NO3 - in soil and decreased soil PH resulted in increased plant parameters. Soil chemical parameters and plant growth parameters were differed by different source of fertilizers industry effluents and follow : ammonia line < urea line < collection basin < tap water. Data also revealed that tomato plant irrigated with water of ammonia line and filtered through Rice Husk Ash + sand filter showed best growth parameters as compared with other plants with higher shoot dry weight of 11.69 g plant - 1. The pot experiment suggested the possibility to using the water of ammonia line which filtered through Rice Husk Ash + sand filter with 50% of nitrogen recommended level to enhance tomato growth and it would save costs on fertilizer and reduction in pollution load of soil and water.

المكافحة الاحيائية لمرض الذبول الفيوزارمي في الطماطا المتسبب عن الفطر Fusarium oxysporum f.sp.lycopersici باستخدام الفطر Trichoderma harzianum وفطر المايكورايزا Glomus mosseae == Biological control of tomato Fusarium wilt caused by Fusarium oxysporum fsp.lycopersici by using Trichoderma harzianum and mycorrhiza Glomus mosseae

Author name: سلام نجم عبود الاسدي
Supervisor name: يحيى عاشور صالح
General topic: Agricultural sciences
Specific topic: Plant - Prevention
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت هذه الدراسة في مختبرات قسم وقاية النبات /كلية الزراعة / جامعة البصرة خلال الفترة من 1/11/ 2015 الى 1/6/2016 لتقييم كفاءة فطر المايكورايزا G. mosseae والعامل الاحيائي T.harzianum في مكافحة مرض الذبول الفيوزارمي لنبات الطماطا المتسبب عن الفطر Fusarium oxysporum fsp.lycopersici . اظهرت النتائج دور العامل الاحيائي في تثبيط نمو الفطر الممرض بطريقة الزراعة المزدوجة حيث كان التضاد من الدرجة 1 حسب مقياس Bell . كما اظهر راشح الفطر الاحيائي T.harzianum تفوقوااضحا في تثبيط نمو الفطر الممرض . اوضحت نتائج تاثير فطر المايكورايزا G.mosseae والعامل الاحيائي T.harzianum والتداخل بينهما في انبات البذور ونمو نبات الطماطا ( تجربة الاصص) وجود فرق معنوي في النسبة المئوية لا نبات البذور ، اما في ما يخص اطوال النبات بعد ستة اسابيع من الانبات فقد اشارت النتائج الى وجود فروق معنوية بين المعاملات حيث بلغت اطوال النباتات 4.55 و18.22 و14.44 و18.00 و15.33 و15.44 و15.55 سم على التوالي للمعاملات Fusarium oxysporum f.sp.lycopersici(f) وTrichoderma harzianum(T.h) وGlomus mosseae(G.m) ومعاملة التداخل بين العامل الاحيائي وفطر المايكورايزا GT ومعاملة التداخل بين فطر الممرض وفطر المايكورايزا GF ومعاملة التداخل بين العامل الاحيائي وفطر الممرض TF ومعاملة التداخل بين العامل الاحيائي وفطر المايكورايزواالفطر الممرضر GTFقياسا بمعاملة المقارنة التي بلغت 12.33 سم ، وكان اكثر الفطريات تاثيرا في زيادة اطوال النباتات العامل الاحيائي T.harzianum كما حقق التداخل بين العامل الاحيائي T.harzianum وفطر المايكورايزا G.mosseae زيادة معنوية في الوزن الطري للمجموعين الخضري والجذري حيث بلغ الوزن الطري للمجموعين الخضري والجذري 6.27 و0.72 غم على التوالي قياسا بمعاملة المقارنة التي بلغ الوزن الطري للمجموعين الخضري والجذري فيها 2.83 و0.51 غم على التوالي . اما التجربة الحقلية فقد اظهرت نتائجهواجود فروق معنوية في شدة الاصابة بالفطر الممرض حيث كانت اقل شدة اصابة في المعاملة التداخل بين فطر المايكورايزواالفطر الممرض GF اذ بلغت 22.21% تلتها المعاملات TF وGTF حيث بلغت شدة الاصابة فيها 27.77 و35.01% على التوالي قياسا بمعاملة المقارنة البالغة 48.02% ، اما اطوال النبات فقد ازداد بشكل معنوي في المعاملة GTF اذ بلغت 72.1 سم والتي تفوقت بفروقات معنوية عالية تلتها المعاملات GT وG وGF وT اذ بلغت 69.9 و68.9 و65.4 و63.8 سم على التوالي اذ اختلفت معنويا عن معاملة المقارنة التي بلغت 55.3 سم ومعاملة الفطر الممرض البالغة 47.2 سم ، وكان اعلى وزن طري للمجموع الخضري في المعاملة TF اذ بلغت 40.0 غم تلتها المعاملات GF وG وGTF وGT حيث بلغ الوزن الطري لها 35.0 و34.5 و32.3 و30.0 و29.7 غم / نبات والتي اختلفت معنويا عن معاملتي المقارنة والفطر الممرض اذ بلغت 17.33 و13.7 غم . وان اعلى زيادة في الوزن الطري للمجموع الجذري بلغت 29.53غم لمعاملة المايكورايزا G.mosseae والتي اختلفت معنويا عن معاملة المقارنة البالغة 17.7 غم . اشارت النتائج الى دور فطر المايكورايزا G.mosseae في زيادة وزن حاصل الثمار اذ بلغ الوزن 3.067 كغم / نبات قياسا بمعاملة المقارنة التي بلغت 1.630 كغم / نبات وبفارق معنوي ، وبينت النتائج دور فطر المايكورايزواالعامل الاحيائي في زيادة الفعالية الانزيمية لا نزيم البيروكسديز فقد اعطت معاملة فطر المايكورايزا G.mosseae اعلى نسبة اذ بلغت 1.710 وحدة / غم وزن رطب وقد اختلفت معنويا عن معاملة المقارنة البالغة 0.510 وحدة / غم وزن رطب .اوضحت نتائج التجربة الحقلية ايضا كفاءة العامل الاحيائي وفطر المايكورايزا في زيادة كمية الفسفور في نباتات الطماطا حيث بلغت اعلى نسبة للفسفور في معاملة التداخل GF حيث بلغت 38.93 ملغم / كغم تلتها المعاملات G وT وGT وGTF وTF وF اذ بلغت نسبة الفسفور فيها 38.44 و28.93 و28.93 و26.58 و23.91 و13.25 ملغم / كغم على التوالي قياسا بمعاملة المقارنة اذ بلغت 21.23 ملغم / كغم. وبينت النتائج دور فطر المايكورايزا G.mosseae والعامل الاحيائي T.harzianum في زيادة نسبة وشدة اصابة الجذور حيث بلغت اعلى نسبة وشدة اصابة لمعاملة التداخل GT 66 و58% على التوالي وقد تفوقت بفروقات معنوية عالية تلتها المعاملات G وGTF وGF حيث بلغت نسبة وشدة الاصابة فيها 38.00 و36.66% و37.66 و34 % و31.33 و32.00% على التوالي . وبينت النتائج ايضواجود فروقات معنوية في عدد ابواغ فطر المايكورايزا G.mosseae حيث بلغ اعلى عدد للابواغ 45.83 بوغ / نبات في معاملة التداخل GT تلتها المعاملات GF وG وGTF حيث بلغ عدد الابواغ فيها 29.16 و22.50 و16.66 بوغ / نبات . | This study was conducted in the laboratories of the Department of Plant Protection , College of Agriculture , University of Basra .during the period from 1/11/2015 - 1/6/2016 ,to evaluate the interaction efficiency between Glomus.mosseae and bio agent Trichoderma.harzianum against tomato fusarium wilt disease caused by Fusarium oxysporum f.sp lycopersici . .The results showed the role of T.harzianum in inhibiting the growth of pathogenic fungus where the antagonism degree is of class 1 according to Bell scale. Also it showed that T.harzianum exaudate inhibited the growth of pathogen. The results also explained a significant effect of mycorrhiza G.mosseae and T.harzianum and their interaction on seed germination percent and growth of tomato plant (pots experience) , As regards to the lengths of the plants after six weeks from germination the results indicated a significant difference among the treatments , the lengths of plants reached 4.55 ,18.22 , 14.44, 18.00, 15.33, 15.44, 15.55, cm for the treatments , F, T ,M, MT , MF , TF and MTF, respectively compared to control which amounted to 12.33 cm ,T.harzianum led to increase the lengths of Plant significantly .The interaction between T harzianum and G.mosseae (TM ) also led to increase the fresh weight in comparison with control which reached respectively 2.83 and 0.51. The field experiment results showed significant differences in the severity of the infection with pathogen where the less severe of the infection was found in the treatment MF which was 22.21 % followed by TF and MTF treatments which were 27.77 and 35.01% respectively. The plant lengths also significantly increased , it reached 72.1 cm in the treatment MTF followed by the treatment MT , M , MF and T which reached 69.9, 68.9 , 65.4 and 63.8 respectively , which they significantly differed with control and pathogen treatments which were 55.3 and 47.2 cm respectively . The treatment TF led to increase the shoot fresh weight up to 40.0 gm. , followed by MF , M,T, MTF and MT which were 35.0, 34.5 , 32.3 , 30.0 and 29.7 which significantly differed with control and pathogen treatment which reached 17.33 and 13.7 respectively . The root fresh weight also increased when G.mosseae was used , it reached 29.53 gm. compared with control (17.7 gm.).The results indicated the role of G.mosseae in increasing fruit weight which reached 3.067 kg Plant compared with control which was 1.630 kg/plant .The results also showed the role of G.mosseae in increasing the enzymatic activity of peroxidase which reached 1.710 unit/gm. Compared to control which reached 0.510 unit/ gm wet weight . The results of field experiment also revealed that T.harzianum and G.mossese increased the amount of phosphor in tomato Plants in a percent of 38.93 , 38.44 , 28.93 , 28.93 , 26.58 , and 23.91 mg / kg for the treatment MF , M , T , MT , MTF and TF respectively in with comparison with control which was 21.23 mg / kg. From other hand , the interaction between G.mosseae and T.harzianum led to increase the root infection percent and infection up to 66 and 58% respectively the treatment M, MTF , MF as they were 38.00 , 36.66% 37.66 , 34% 31.33 , 32.00% . Finaly the results elucidate that the interaction between G.mosseae and T.harzianum led to increase the number of spores of G.mosseae reaching to 45.83 spore/ plant followed by the treatment M , MTF , MF , which gave 29.16 , 22.50 , and 16.66 , spores / plant respectively
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