التشكيل الوراثي لجين FASN وعلاقته لانتاج الحليب ومكوناته في سلالتي الابقار الجنوبي الفريزيان == FASN Gene Polymorphism and its relation to the production milk and components in the ALJnoby cattle breeds and friesian
Author name:
غفران ماجد عزيز مارد النيازي
Supervisor name:
اسعد يحيى عايد
General topic:
Agricultural sciences
Specific topic:
Zoology - Education
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1841 - p.pdf
Abstract:
Atotal of 60 cows belong to freisian local Iraq breed (30 each ) were used in the cow for aperioed of 1/12/ 2012 to 30 /3 /2015 cows were from local breeders within Thi - Qar provin samples were callectted from each animal one hrough study period . After measuring milk asmples of 50 ml were taken to estimate fat , protein and some other milk composition as Well as fatty acid composition were estirm from extracted fat Besides that blood samples from each animal were taken to extract agene was amplicied cat in to different results can be summarized as follow : 1 - FASN gene has been amplified and there were identified .Genotypes were AA,AG and frequency of 0.13 ,0.54 and 0.33 respeccfin freisian breed and 0.47 ,0.33 and 0.16 of A and G alleles frequencies were 0.40 and 0.60 of freisian breed and 0.65 and 0.35 of local breed respechlivy the two population were at aqulibirium .2 - Breeds showed similar obsereved number of alleles (2) observied herrozygosity differe between breeds (53.3% and 36.5 for freisian and local breed respectively ). AHowver showed similar expected heterozygosity (48.81 and 46 .27% respectirely ).3 - AA and GG genotypes excceded the genotype AG by daily and total milk yield of freisian breed . they produced 2968.90 and 2809.80 kg total milk yield respectively .AG gene produced only 2645.54 kg .4 - AA genotype of freisian were produced significantly higher fat and protein (97.10 and 100.05 kg respectively ) that of other genotypes .As well as AA and AG genotypes produced singnificantly higher fat% (3.27% and 3.29% respectively ) in comparicer with 3.17% of GG genotypes the AG genotype produced highest protein% (3.4170)in comparison with other genotypes.5 - AA genotypes of local breed exceeded other genotype in producing daily and total milk yield ,fat and protein.on contrary ,GG genotype yielded higher fat %(4.2) in comparison with that of other genotypes AA and GG genotypes have got highest level of protein (3.25 and 3.30% respechvely ) in comparison with 3.20% AA genotype .B6 - Only AG and GG of local breed were strain in the registration of fat content protein within the standard range ,wich is 1.23, 1.27 respectire .7 - Daily milk breeds value s. recorded negative breedes values.there was an indicater that A allele has positive effecf on daily milk yield .hower A allel has negative effect.8 - A allele has negative effect on fat % in milk cows consequently genotypes have this allele (AA and AG) had negative breeders values. On the other hand ,AG and GG genotypes of local breed had negative breed ing values .9 - GG genotypes of both breeds had positive protein % breeding value ,although its breeding value was nearly zero .10 - GG genotype of Friesian breed produced higher palametic and oleic fatty acids than those of AA and AG genotypes . whereas , AA and AG genotypes produced highes level of palametic and total saturated fatty acid .11 - Genotypes of FASN gene of local breeds did not show significant differences amang all types of milk fatty acid .12 - Freisian exceeded local breed to quantity and quality of milk specially fatty acid composition that has positive effect on human health.C
