Author name: رشاد عادل عمران الزبيدي

Supervisor name: هيفاء جاسم حسين التميمي | عبد الزهرة طه ظاهر

General topic: Agricultural sciences

Specific topic: Soil and Water - Fertility

Degree: Doctorate

University: University Of Basrah - Faculty Of Agriculture

Language: Arabic

University location: Basrah

First pages:

استخدام بعض المرشحات في خفض الملوحة والبورون في مياه شط العرب وابار جنوب البصرة لاستعمالها في ري نبات الطماطة Lycoperscion esculentum Mill == Using of Some Filters in Dercreasing Water Salinity and Boron Toxicity of Satt Al - Arab River and Basrah Southern Wells and Reuse of Tomato Plant (Lycoperscion esculentum Mill) Irrigation

Author name: حنان عبد الوهاب سعيد الحكيم

Supervisor name: هيفاء جاسم حسين التميمي | نجلة جبر محمد الاميري

General topic: Agricultural sciences

Specific topic: Soil and Water

Degree: Doctorate

University: University Of Basrah - Faculty Of Agriculture

Language: Arabic

University location: Basrah

First pages:

Abstract: Thirteen water sample of Shatt Al - Arab river and its tributaries were collected starting with Madania at (north of Basrah ending with Al - Fao south of Basrah) according to the following areas (Medania , Gurna , Sharesh, Deyar/ near paper plan, Hartha (near thermal electric station), Garmat Ali (Basrah university), Tenoma/Sahehia , near teaching hospital, Abul - Khaseeb /Hamdan, Abul - Khaseebl Abu - Floose, Sebha, Al - Dora, and Al - Fao. Fifteen well's water sample were collected from Al - Zubair , Al - Berjesia and Safwan during Dec.2014. Chemical and physical properties of water samples were conducted (ECiw), soluble ions (calcium, mangnesium, sodium, potassium, chloride, sulphate, carbonate, bicarbonate, boron, and nitrate), total dissolved solids (TDS), and total hardness. After water sample properties determination, they were classified to three classes according to electrical conductivity (1 - 4, 4 - 8, and 8 - 12) dSm - 1 , and three classes of boron concentrations (< 0.7, 0.7 - 3.0, and > 3.0) mg L - 1 . For the purpore of study of studies filter efficiency, two type of filter were used, mineral filter (sand, charcoal, porcelin, and cement klint dust) and organic filters (rice hust ash, barley straw, sawdust, plam leaf, and poultry rnanure). Two experiments were conducted, one of them laboratory experiment in soil science and water resources laboratories /College of Agriculture /University of Basrah) other was biological experiment in wooden canopy to achieve the objectives of the study.Nine type of mineral and organic types of filters were selected for studying their efficiencies in decreasing water salinity and boron toxicity of Shatt Al - Arab river and Wells, after passing water through them. Chemical and physical properties of water were determined after passing water through filters and water quality was calculated. A number ofBcriteria and international standards were used for evaluation water quality after and before passing through filters.For studying the efficiency of water treatment by the most efficient filters according to laboratory experiment results were rice husk ash, cement klint dust, sand, and control treatment. Biological experiment was conducted by using plastic pots with capacity of20 kg soil according to factorial experiment with three replicates for each treatment with 24 empirical units (2×3×3) (soil * No. of filters * replicates) besides control treatment and six classes of water to be 144 empirical units. Two soils were used one of them from Agricultural field of Agricultural Research Station, and the other from Garmat Ali field during agricultural season 2014 - 2015. Mineral fertilizers were used with rates, 320 Kg N ha - 1as urea (46 % N) with two doses, phosohate fertilizer was applied at rate 90 kg P ha - 1 as concentrated supper phosphate (20.21 % P), potassium at rate of 120 kg K ha - 1 as potassium sulfate (43 % K) one dose at planting. Peatmose fertilizer was applied at rate 2% for all treatment mixing with soils. For getting erough water for irrigation, plastic coloumns wereduplicated in their dimentions for studied filters. Shatt Al - Arab and wells water were passed through most efficient filter besides sand filter separately with equal quantities. Water was collected after one minute to conform the properties of laboratory experiment.Tomato plants (Lycopersiconesculintium M.)were planted on 22/9/2014 and irrigated with water treated with filters besides control treatment at field capacity (20 % for Berjesia Research Station soil) and (30 % for Garmat Ali soil). Leaching requirement was calculated for each treatment. After 176 days, plants were harvested with space 2 cm from soil surface avoiding contamination. Tomato plants were fractionated into three parts, roots, shoots, and fruits. Dry matter of roots and shoot wasBCcalculated. Boron concentrion of (roots, shoots and fruits) was determined. Fruits yied of tomato was calculated. Electrical conductivity and available boron of residual soils in pots were determined after tomato plants growth Period.Results obtained could be summarized as : 1. Adoption of rice husk ash and cemenentklint dust in treatment of Shatt Al - Arab and wells water because of their high efficiency in decreasing boron concentration and water salinity and reuse for tomato plant irrigation and Plants irrigated with water treated with rice husk ash were surpassed over other plants in dry matter and yield, and gave lowest boron concentration in tomato plant tissues for all parts roots, shoots, and fruits. Same treatment gave the lowest concentration residual boron in soil and lowest value of soil salinity as compared with water treated with cementklint dust filter and sand filter besides control treatment.2. loamy sand soil was surpassed over clay loam soil in dry matter and yield of tomato and lowest concentration of boron in their tissues, while clay loam soil was surpassed over loam sand soil in residual boron concentration and soil salinity.3. Results indicated that there were significant differences between type of filters and soil texture in dry matter, yild , boron concentration in roots , shoots, and fruits of tomato plants. Residual boron concentration and soil salinity.4. Results indicated that there was no boron contamination in both studied soils treated with water of Shatt Al - Arab and wells treated with two filters (rice husk ash and cementklint dust)

تاثير بعض معاملات السيطرة الطبيعية قبل الحصاد وبعده في تحسين الصفات النوعية والقابلية الخزنية لثمار نخيل التمر Phoenix dactylifera L. صنفي البرحي والبريم == EFFECT some pre and post - harvest natural control treatments on improving the qualitative characteristics and storage ability of date palm fruits (Phoenix dactylifera L. ) cvs. Barhi and Bream

Author name: حمزة عباس حمزة عباس

Supervisor name: عباس مهدي جاسم | ضياء احمد طعين

General topic: Agricultural sciences

Specific topic: Plant - Gardening

Degree: Doctorate

University: University Of Basrah - Faculty Of Agriculture

Language: Arabic

University location: Basrah

First pages:

Abstract: Research were divided into two experiments, field and storage , field experiment was conducted in private orchard at Abu - Alkhasib south of Basrah . Date palm fruits( Phoenix dactylifera L.) cvs. Barhi and Bream were used during the growing seasons 2014 and 2015. The aim of the study was to investigate the effect of spraying chitosan at the concentrations of 0% , 1% , 2% on yield and some physical , chemical and physiological characteristics of fruits. The experiment included 9 trees for both cvs. Complete Randomized Block Design was used with three replicates . The results were analyzed by the analysis of variance of factorial experiment and mean values were compared using the Revised Least Significant Difference Test at 0.05 probability level. The results of study showed significant effect of spraying chitosan at the concentrations of 2% on the most of the studied parameters (fresh weight , size of fruit , yield , water content, total acidity ,tannin and pectin) in addition to delay the ripening and respiration rate of fruits for both cvs. Untreated fruits retain the highest percentage of total soluble solids , total sugars , reducing sugars and total protein. Invertase and cellulase activities decreased in fruits treated with chitosan as compared with untreated fruits which recorded the highest activity of invertase and cellulase for both cvs.Storage experiment was conducted in Al - Moussawi dates factory situated at Abu - Al - Khaseb District , Basrah Governorate to study the effect of some pre and post harvest natural control treatments in improving the qualitative characteristics and storage ability of date palm fruits cvs. Barhi and Bream. Fruits that treated with chitosan at the concentrations of (0, 1, 2) % were harvested in the early morning and carried to Al - Moussawi dates factory. Then every one of the three parts divided into five groups ,the first was treated with ultraviolet light (UV) at the concentration of 1KGY for periods of (0, 5, 10) minutes ,while the second group were treated with (1 - MCP) at the concentration of ( 0,0.5,1) ppm for 24 hour at 0 ºc .third was treated with ozone (O3) at the concentration of 5 ppm for the periods of (0, 0.5, 1) hour ,while the fourth group were soaked in chitosan at the concentrations of ( 0,1,2) % , the fifth were without treatment .Fruits were packed in plastic bags.Both of the two parts were stored at two storage temperatures (room temperature 25±2ºc and freezing temperature - 10±2ºc for three weeks and six months respectively . Storage characteristics were studied before andBduring storage and at the end of storage period . the results can be summarized as follow : 1 - spraying chitosan at the concentrations of 2% in the field reduced the percentage of deteriorated fruit , ripening process, TSS , total and reducing sugars and increased the percentage of water content, sucrose and total acidity in addition to elongation the desirable color of fruits and keeping good flavor and taste for Barhi and Bream fruits until the end of storage period at - 10±2ºc and 25±2ºc for the both of growing seasons 2014 and 2015 .2 - Treating with ultraviolet light (UV) for 5 minutes reduced percentage of decay for Barhi ,whereas fuel west percentage of Bream fruits decay was in fruits treated wish ozone for 1hour for both growing seasons .3 - Treating with (1 - MCP) at the concentration of (1) ppm reduced the progress of ripening and total sugars and increased water content, sucrose and total acidity in addition to elongation the desirable color of fruits and keeping good flavor and taste for Barhi and Bream fruits up to the end of storage period at - 10±2ºc for the both of growing seasons .4 - The percentage of decay of fruits , ripening progress, loss in weight , TSS , total and reducing sugars increased whereas the percentage of water contents, sucrose and acidity decreased with increment of storage period for both cultivars and seasons at 25±2ºc and - 10±2ºc.5 - The interaction between field treatments and storage treatments had significant effect on percentage of fruits decay. Fruits sprayed with chitosan at the concentrations of 2% in the field and treated with ultraviolet light (UV) for the periods of 10 minutes had the lowest percentage of fruits decay for both growing seasons .6 - The interaction between spraying chitosan and storage period significantly effected on studied parameters . Fruits sprayed with 2% chitosan in the field were superior in keeping water content and delaying ripening process but they had the lowest TSS , total and reducing sugar at the end of storage period at 25±2ºc and - 10±2ºc compared with untreated fruit for both cultivars and seasons .7 - The interaction between storage treatments and storage period significantly effected on studied parameters. Fruits treated with (1 - MCP) at the concentration of (1) ppm were the best in keeping water content and delaying ripening process but they had the lowest TSS , total and reducing sugar at the end of storage period at 25±2ºc and - 10±2ºc compared with untreated fruit for both cultivars and seasons .C8 - The interaction among field treatments, storage treatments and storage period had significant effect on percentage of fruits decay. Fruits sprayed with chitosan at the concentrations of 2% in the field and treated with ultraviolet light (UV) for the periods of 10 minutes had the lowest percentage of fruits decay , at the end of storage period at - 10±2ºc for both cultivars and seasons .9 - Gel electrophoresis for protein showed that in Barhi and Bream fruits treated with pre and post harvest natural control treatments and stored at - 10±2ºc , the process of gene expression has occurred , since there was a number of bands , which differed both in number as well in their position on the gel , depending on treatments and cultivar . There was very clear differences between treatments in the number , position and the timing of the appearance of the protein bands for both cvs. during which Bream fruits treated with chitosan recorded the highest number of bands ( seven bands).

التوصيف الجزيئي والتعبير الجيني لجين بروتينات الصدمة (الحرارية 00 hsp70) في السائل المنوي لثيران الهولشتاين العراقية والكباش العرابية == Molecular Characterization and Gene Expression of Heat Shock Protein 70 (hsp70) Gene in the Semen of Iraqi Holstein Bulls and Arabi Rams

Author name: حسن نعمة حبيب السنيسل

Supervisor name: عماد فلاح حسن | بسام ياسين خضير

General topic: Agricultural sciences

Specific topic: Zoology

Degree: Doctorate

University: University Of Basrah - Faculty Of Agriculture - Department Of Livestock

Language: Arabic

University location: Basrah

First pages:

Abstract: This study was conducted at the Animal Farm of College of Agriculture, University of Basrah, (longitude 47.7433690, latitude 30.5627250 north of Basrah), and the fields of Artificial Insemination Center in Abu Ghraib, the General Company for Livestock Services (longitude 44.1922070, latitude 33.3095550 northwest of Baghdad), during the winter season for the months November, December 2015 and January 2016, and the summer season for the months April, May and June, 2016. To achieve all the study requirements, the laboratories work at the Laboratory physiology, Molecular Genetics Laboratory, College of Agriculture, University of Basrah; the Central Research Unit, College of Veterinary Medicine, University of Basrah; the Criminal Laboratory Department of Investigation, Criminal Evidence Directorate, Basrah Governorate Police; as well as laboratories of the Abu Ghraib, Artificial Insemination Center; in addition to the First BASE Laboratories, Laboratory in Selangor Malaysia, were used. The study included two experiments : The first experiment : This experiment was designed to detect the genetic polymorphism of hsp70 gene in the semen of Bulls, their relationship to certain physical and chemical semen characteristics, the level of gene expression in the season winter and summer, and the evaluation of the bulls by finding the breeding value of certain traits in the fresh and frozen semen of the Iraqi Holstein Bulls, back to the Artificial Insemination Center / Abu Ghraib / Baghdad, 30 bulls were used in this experiment.Second Experience : This experiment was designed to detect the genetic polymorphism of hsp70 genes in the semen of Rams, their relationship with some physical and chemical semen characteristics, the level of gene expression in the season winter and summer, and the evaluation of the rams by finding the breeding value of some traits in the fresh and frozen semen of local Arabi rams, back to the Agricultural Research Station / College of Agriculture / University of Basrah, 20 Rams were used in this experiment.Results of the study : I. The first experiment : 1) After DNA extracted, the quantity and purity of the DNA were determined by the Nano drop device, the purity ratio was 1.8. A Polymerase Chain Reaction (PCR) carried out, the amplification product was approximately 1926bp. Nucleotide Sequence analysis was done to detect polymorphism and compared with the genotypes of the hsp70 gene in the Gene Bank. There are three Genotypes of hsp70 gene were obtained in bulls : : The Genotype A, this genotype is the closest to heat shock protein hsp70 gene in the Holstein bulls in Gene Bank. This polymorphism was exposed to be genetic mutations at position No. 6, the Nucleotide (C < G), No. 174 (C < G), No. 282 (A< G) and position No. 1339 (T < C). All mutations are silent that did not encodingto any new amino acid, (16 Bulls).B : The Genotype B, the nucleotides were substituted at different positions, position No.114 (G < A), No. 174 (C < G), No. 282 (A < G), No. 1339 (T < C),No.1451 (C < A), No.1590 (A < G), No. 1695 (C < T) and position No. 1719 (G<T), these genetic mutations are all silent except the mutation at the position No.1451 is a missense mutation, encoding for new amino acid, (6 Bulls).C : The Genotype C , the nucleotides were substituted at different positions, position No. 114 (G < A), No. 174 (C < G), No. 282 (A < G) No.1339 (T < C),No. 1590 (A <G), No. 1695 (C <T) and position No. 1719 (G <T), all mutationsare silent, ( 8 bulls).2) The results of the Multiple Sequence Alignment (MSA) of hsp70 gene in the bulls showed that the convergence ratio of A, B and C were 99.73%, 99.51% and 99.56%, respectively, with the same gene in the Holstein bulls recorded in Gene Bank. The MSA results also showed that the B genotype gave the lowest ratio to the studied genotypes, followed by the C genotype and the A genotype, which showed the highest approximation with the genotypes studied in the Gene Bank for the Holstein bulls, sheep, goats, pigs and humans.3) Genotype B, has shown significantly higher (P < 0.05) than the C genotype and A genotype in all physical semen characteristics studied, it gave the highest rate of mass and individual motility, the highest percentage of live sperm and thelowest rate of abnormalities, both in fresh and frozen semen in a period of onemonth and two months freezing and in both seasons of the study.4) Genotype B showed Significantly higher (P < 0.05) towards the C and A genotypes in all chemical semen characteristics studied, It gave the highest percentage of the Acrosome integrities and gave the lowest level of concentration of enzymes ALT, AST and ALP in the frozen semen and freezing periods in a month and two months and in both seasons of the study.5 ) After RNA extraction, synthesise of cDNA and carry out Quantitative Realtime PCR (qRT - PCR) analysis, genotype B showed Significantly higher (P < 0.05) than genotype C and A in the level of gene expression of heat shock protein hsp70 gene in the summer season.) The bulls belong to genotype B were superior in the valuation on the basis of the breeding value of motility, live sperm and the abnormalities in the fresh and frozen semen for a month and two months followed by the C than A.II. Second Experience : 1) After DNA extracted, the quantity and purity of the DNA were determined by the Nano drop device, the purity ratio was 1.8. A PCR carried out and the amplification product was in approximately 1926bp, bands were visualized by gel electrophoresis. Nucleotide sequence analysis was done to detect polymorphism compared to that of the hsp70 gene in the Gene Bank. There are two genotypes ofhsp70 gene were obtained in rams : A : The A genotype, showed no match with other nucleotides sequence of other group neither heat shock protein hsp70 gene in Gene Bank, the nucleotide in position 1308, the nucleotide T was substituted by C (T < C), a silent genetic mutation that did not encoding a new amino acid, (10 rams).B : The B genotype, in comparison with the A genotype , the Nucleotide C was substituted with G (C <G), a missense genetic mutation that resulted from change the amino acid encoding at the site 514, a genetic mutation not previously recorded in rams except in inese sheep. In addition, nucleotide G was substituted by nucleotide A (G < A) in the position 1524, a silent genetic mutation (10 rams).2) The results of the (MSA) of hsp70 gene in the rams showed that the ratio of Aand B genotypes were 99.89% and 99.84% respectively with the same gene inrams in the Gene Bank. The MSA of the genotype B gave the lowest ratio to thesame gene in sheep, Holstein bulls, goat, pig and human3) The genotype B Significantly higher (P <0.05) than the genotype A in allPhysical semen characteristics studied, it showed the highest rate of mass andindividual motility and the highest percentage of live sperm and the lowest rate ofabnormalities, in fresh and frozen semen in a period of a month and two monthsfreezing and in both seasons of the study.4) The genotype B Significantly higher (P <0.05) than the genotype A inall chemical semen characteristics, it showed the highest percentage of theAcrosome integrities, and showed the lowest level of concentration of enzymesALT, AST and ALP in the frozen semen and freezing periods of month and twomonths and in both seasons of the study.

تنقية وتوصيف انزيم اللايبيز المستخلص من بذور فول الصويا المنبتة واستخدامه كمحسن للخبز == PURIFICATION AND CHARACTERIZATION OF LIPASE EXTRACTED FROM SOY BEAN SEEDS AND USED IT AS IMPROVER IN BAKING

Author name: بتول محمود محمد الانصاري

Supervisor name: علي احمد ساهي | ضياء فالح الفكيكي

General topic: Agricultural sciences

Specific topic: Food

Degree: Doctorate

University: University Of Basrah - Faculty Of Agriculture

Language: Arabic

University location: Basrah

First pages:

Abstract: تضمنت الدراسة استخلاص وتنقية انزيم اللايبيز من سبعة انواع من البذور الزيتية قبل وبعد الانبات وهي(فول الصويواالسلجم وزهرة الشمس والعصفر والدخن والذرة الصفراء والخروع). اظهرت نتائج تقدير الفعالية النوعية ان افضل نوع للبذور الزيتية هو بذور فول الصويا اذ اعطت اعلى فعالية نوعية للانزيم في البذور قبل الانبات وبعد الانبات 3.58 و11.20 وحدة / ملغم بروتين على التوالي مقارنة مع بقية الانواع الاخرى. استخلص اللايبيز من بذور فول الصويا قبل وبعد الانبات باستعمال تسعة محاليل استخلاص ووجد ان افضل محلول للاستخلاص هو محلول Tris - HCl تركيز 0.1 مولاري الحاوي على 0.01 مولاري كلوريد الكالسيوم و0.001 مولاري اثلين داي امين تترا استك اسد و0.001 مولاري كلوريد المغنسيوم وبدالة حامضية 8 , اذ انه اعطى اعلى فعالية نوعية. درس التركيب الكيميائي لبذور فول الصويا قبل الانبات وخلال مدة الانبات المختلفة اذ لوحظ ارتفاع نسبة البروتين في البذور المنبتة لتصل ذروتها عند نهاية اليوم الخامس من الانبات والذي بلغ 41.60% مقارنة بنسبة البروتين قبل الانبات 35.57 %. اما نسبة الدهن والكاربوهيدرات والرماد فقد انخفضت في البذور خلال مدد الانبات اذ بلغت 17.75 و30.18 و5.97 % على التوالي مقارنة بالبذور غير المنبتة 20.74 و32.5 و6.26 % على التوالي. تم متابعة تطور اللايبيز في مدة الانبات لبذور فول الصويا, اذ امتلك اعلى فعالية في اليوم الخامس من الانبات حيث بلغت 38.00 وحدة/ مل . اجريت خــــــــــــطوة الترسيب التدريجي للانزيم باستعــــــــمال نسب اشباع متدرجــــــــــــــــة من كبريتات الامونيوم 30 - 90 %, اذ لوحظ حدوث ارتفاع واضح بشكل تدريجي للفعالية النوعية للانزيم في الراسب الناتج لغاية نسبة اشباع 80 % وقد اعطت هذه الخطوة فعالية نوعية مقدارها 324.44 وحدة / ملغم بروتين وحصيلة انزيمية بلغت 29.20 % بعدد مرات تنقية مقدارها 10.78 مرة. اما خطوة التنقية بالترشيح الهلامي باستعمال عمود الترشيح الهلامي Sephadex G - 100فقد بلغت الفعالية النوعية 366.66 وحدة/ ملغم بروتين وبحصيلة انزيمية 17.83% وبعدد مرات تنقية 12.18 مرة. وبينت نتائج تحديد نقاوة اللايبيز ظهور حزمة بروتينية واحدة عند الترحيل الكهربائي في هلام متعدد الاكريلامايد بغياب العوامل الماسخة, في حين بلغ الوزن الجزيئي 41.687 كيلو دالتون بطريقة الترحيل الكهربائي في هلام متعدد الاكريلامايد بوجود العوامل الماسخة . وجد ان الدالة الحامضية المثلى لفعالية الانزيم كانت 8 , في حين تراوحت الدالة الحامضية المثلى لثبات الانزيم بين 7 - 9 اذ احتفظ الانزيم بـ 90 % من فعاليته. بينما لوحظ ان درجة الحرارة المثلى للفعالية الانزيمية للانزيم المنقى هي 40 م وان الانزيم يفقد 67 % من فعاليته على 90 م . اظهرت نتائج تاثير الايونات المعدنية والعوامل المختزلة والكلابية على فعالية اللايبيز ان لايونات الزئبقيك والخارصين والحديدوز بتركيزي 1 و5 ملي مولاري تاثير تثبيطي في فعالية الانزيم ،اذ بلغت الفعالية المتبقية 63.2 و68.9 و66.1 % على التوالي, في حين احتفظ الانزيم بكامل فعاليته عند تركيز 5 ملي مولاري بوجود ايونات الكالسيوم والبوتاسيوم. كمااظهرت العوامل الكلابية والمختزلة ان للـ EDTA دورا تنشيطيا عند حضنه مع اللايبيز بتركيز 1 و5 ملي مولاري. ولوحظ ان اللايبيز لا ينتمي الى مجموعة الانزيمات الفلزية (metalloenzyme). اما اليوريا فكان لها دور تثبيطي على فعالية الانزيم عند تركيز1 و5 ملي مولاري, في حين بلغت الفعالية المتبقية للانزيم 86.2 % عند حضنه مع 1% Mercaptoethanol 2 - , بينما كانت الفعالية المتبقية للانزيم عند حضنه مع Hydrogen peroxide وSodium hypochlorid 74.7 و75.8 % على التوالي بتركيز1 % .ولوحظ ان لمواد الشد السطحي تاثيروااضحا على فعالية الانزيم عند تركيز 1 و5 %, اذ احتفظ الانزيم بكامل فعاليته عند حضنه مع Triton X - 100 وTween 20عند تركيز 1% . في حين تاثرت فعالية اللايبيز باضافة المنظفات التجارية, ولكنها اظهرت ثباتية عالية في وجود المنظف Sar حيث بلغت الفعالية المتبقة 99.1 %. بينت النتائج تباين النسبة المئوية للاحماض الامينية الموجودة في اللايبيز اذ لوحظ ان اعلى تركيز كان لليوسين Leu بلغ 16.4 % بينما اقل تركيز كان للاسبارتك ASP 1.5 % في حين تفاوتت الاحماض الامينية الاخرى في نسبها المئوية. اظهرت نتائج فحص الفارينوغراف وجود فروق معنوية بين قيم متوسطات نسب امتصاص الماء للمعاملات واظهر تركيز 0.004 ملغم لايبيز / 100 غم طحين و0.75 % محسن Ovalette اعلى نسبة امتصاص للماء 61.7 و59.9 % على التوالي , في حين ازدادت فترة الاستقرار معنويا 9.6 و9.9 دقيقة عند تركيز 0.002 و0.004 ملغم لايبيز/ 100 غم طحين و13.3 و11.6 دقيقة عند تركيز0.50 و0.75 % محسن. اما قيم متوسطات فترة النضج للعجين فقد ازدادت مع زيادة تركيز الانزيم 0.002 و0.004 ملغم لايبيز/ 100 غم طحين معنويا اذ كانت 7.2 دقيقة وارتفعت فترة نضج العجين عند جميع تراكيز المحسن 6.8 و8.5 و6.2 دقيقة على التوالي مقارنة مع العجين الخالي من اية اضافة. وجد من العلامات النهائية للتقويم الحسي ان افضل معاملة كانت عند اضافة اللايبيز بتركيز 0.002 ملغم لايبيز/ 100 غم طحين و0.5 % Ovalette وكان المجموع النهائي للمعاملات 95 و94 % على التوالي. لوحظ من نتائج فحوصات تاثير اللايبيز على تجلد الخبز ان قيم كل من حجم الراسب في عالق اللب المائي وقوة التشرب ورطوبة اللب انخفضت تدريجيا مع زيادة مدة حفظ الخبز, اما نسبة الرطوبة في القشرة فقد ازدادت مع تقدم مدة الحفظ. وكان تاثير اضافة اللايبيز والمحسن Ovalette ايجابيا على الصفات النوعية ( الحجم ولون ونضارة اللب) والخزنية (تاخر تجلد اللب) للخبز المختبري | The study included extraction and purification of lipase enzyme from seven kinds of oil seeds before and after germination, those were (soy bean ,rapeseed, sunflower, safflower, pearl millet, yellow corn and castor bean seeds). The results of spcific activity estimation showed that , the best kind of oil seeds are soy bean seeds, they gave the highest specific activity of enzyme before and after germination 3.58 and 11.20 unit/ mg protein respectively comparing with other varieties. Lipase was extraction from soy bean seeds before and after germination using nine extraction solutions the best extractant solution was Tris - HCl 0.10 M containing 0.01M calcium chloride and 0.001mM ethylene di amin tetra acetic acid and 0.001 mM magnesium chloride pH 8. Lipase improvement was monitored through the germination period of soy bean seeds, it reached the highest activity of lipase after five days of germination ,which was 38.00 unit / ml .The chemical composition of soy bean seeds studied before germination period and through different germination period , higher protein percentage was noticed in the germination seeds where it reached its peak at the end of five days of germination 41.60 % compared with protein percentage before germination 35.57 %. For the percentage of fat, charbohydrates and ash they decreased in the seed through germination periods, down to 17.75 %, 30.18 % and 5.97% respectively compared with non - germinated 20.74 % and 32.50 and 6.26% respectively. The gradually precipitated by addition of ammonium sulphate to final saturation of 30 - 90%. Agradual obvious higher specific activity of the enzyme in the precipitate resulted ,till saturation level 80% , this step gave aspecific activity of 324.40 unit/ mg protein and an enzyme yield of 29.20% ,purification folds 10.79 time. While purification step of gel filteration using Sephadex G - 100 where the specific activity 366.66 Unit / mg protein , enzyme yield of 17.83 % and purification fold 12.18. Results for determination of lipase purity showed an appearance of a single protein band by poly acrylamide gel electrophoresis with out of denaturizing agent . The molecular weight of enzyme was 41.687 kilo dalton by poly acrylamide gel electrophoresis with denaturizing agent . The optimum pH for enzymtic activity of the purified enzyme were 8 while the optimum pH stability profile of the enzyme was between 7 - 9, and the enzyme kept 90% of its activity, while notes that the optimum temperature for the enzymatic activity of the purified enzyme was 40 C ͦ, and that enzyme loses 67% of its activity at 90 C ͦ. The resulte of effectness of metal ions and reducing and chelating agent was showed that ions of mercury and zinc ions and ferrous at concentrations of ( 1 and 5 ) m M have an inhibition effect on enzyme activity, as the remaining activity reached the values 63.2 .68.9 and 66.1% respectively , while the enzyme kept its total activity at concentration of 5 m M in the presence of calcium and potassium ions. The chelating and reducing agents showed that EDTA has an active rule when the enzyme incubated with 1 and 5 mM of lipase. And it was noticed that lipase dosn΄t belong to the metalloenzyme group. For urea , it has an active rule on the enzyme activity at 1 and 5 M ,where remaining activity for the enzyme reached 86.2% when incubated with 1% 2 - mercaptoethanol while it was 74.7 and 75.8 % respectivly when incubate with 1% of Hydrogen peroxide and Sodium hydrochloride respectively. Concentration ,for enzyme kept its total activity when incubated with Triton X - 100 and Tween 20 at concentration 1%. While lipase activity did n΄t effected by adding commercial detergents, it also showed a high stability in present of Sar detergent where as residual activity reached 99.1 % . Results cleared the contrast of amino acids existin lipase because it is noticed that the highest concentration reached by leucine was 16.4% where as lowest concentration was that reached by aspartic acid 1.5% ,the other amino acids differed in their percentage . The results of pharinograph test showed that there were significant differences between the mean of water absorption the ratios showed that the concentration of 0.004 mg / 100 g flour of enzyme and 0.75% improver give highest absorption of water with significant of moral 61.7 and 59.9% respectively, while the stability period increased to 9.6 and 9.9 minutes at a concentration of 0.002 to 0.004 mg / 100 g flour of enzyme and 13.3 and 11.6 minutes at a concentration of 0.50 and 0.75% improver with significant differences. The average values of maturity period of the dough increased with increasing concentration of the enzyme 0.002 and 0.004 mg / 100 g flour with significant difference as it was 7.2 minutes and increased the maturity period of the dough at all improver concentrations of 6.8, 8.5 and 6.2, respectively, compared with the dough without any addition. Found from the final marks of sensory evaluation ,it is found that the best process was when add lipase at 0.002 mg / 100 g flour concentration and 0.5 % Ovallete ,the final marks were 95 and 94 % respectively. Its has been noticed that the values of volume of sediment in the crumb aquous suspended ,absorption power and crumb moisture, all reduced gradually with the increasing of loaf storage time. While the moisture content of crust was increased The effect of adding enzyme and improver Ovallete was positive regarding the specific characteristics of loaf such as ( volume ,color and softness of crumb) for loaf.

دراسة السلوك الوراثي في الذرة الصفراء بتاثير مغنطة البذور وتقدير التباعد الوراثي على المستوى الجزيئي == STUDY BEHAVIOR GENETIC IN MAIZE AT EFFECT OF SEEDS MAGNETIZATION AND ESTIMATION DIVERSITY GENETIC AT MOLECULAR LEVEL

Author name: محمد فالح فيصل

Supervisor name: هاشم ربيع لذيذ | عبد مسربت احمد

General topic: Agricultural sciences

Specific topic: Plant - Production

Degree: Doctorate

University: Al-furat Al-Awsat Technical University - Technical College

Language: Arabic

University location: Baghdad

First pages:

Abstract: اجريت تجربة حقلية في حقل احد مزارعي منطقة مشروع المسيب خلال الموسمين الربيعي والخريفي لعام 2015 والموسم الخريفي لعام 2016 ، شملت الدراسة ثماني سلالات نقية من الذرة الصفراء وهي(Sy - 9 وMGW - 3 وS - 165 وM - 17 وZm - 9 وSy - 22 و7MGW - و(Sy - 1 وقسمت الى اربع سلالات منها كاباء مؤنثة واربع سلالات كاباء مذكرة واجرى التضريب بينهما بطريقة التهجين العاملي المقترح من قبل ComstockوRobinson (1948 و1952) ، زرعت حبوب الاباء في الموسم الربيعي 2015 واجرى التضريب بينهمواتم الحصول على الحبوب لستة عشر هجينا وفي الموسمين الزراعيين الخريفي2015 و2016 قسمت الحبوب الى قسمين الاول تعرض لعملية المغنطة بشدة 1500 كاوس ولمدة 30 دقيقة والقسم الاخر من دون مغنطة زرعت حبوب الاباء الثمان والهجن الستة عشر في تصميم القطاعات الكاملة المعشاة بترتيب الالواح المنشقة شملت عملية المغنطة ومن دون مغنطة الالواح الرئيسية اما التراكيب الوراثية فقد شغلت الالواح الثانوية وبثلاث مكررات لتقدير قوة الهجين وقابلية الائتلاف وبعض المعالم الوراثية ومعدل درجة السيادة ومعرفة تاثير مغنطة الحبوب في صفات النمو الخضري والحاصل ومكوناتة ، ونفذت تجربة مختبرية في مختبرات دائرة فحص وتصديق البذور التابعة لوزارة الزراعة لدراسة التباعد الوراثي بين السلالات المدروسة. واظهرت نتائج التجربة الحقلية وجود فروق عالية المعنوية بين متوسطات الصفات المدروسة بتاثيرعملية المغنطة وعدمها للتراكيب الوراثية وتداخلهما ماعدا صفة عدد العرانيص كانت معنوية للموسمين. تراوحت قوة الهجين بين القيم السالبة والموجبة اذ اعطى التضريب (MGW - 3×MGW - 7) اعلى قوة هجين موجبة بلغت 188.17 و181.25 % لصفة حاصل النبات بوجود وغياب عملية المغنطة للموسم الاول والثاني بالتتابع ، واظهرت الاباء المؤنثة افضل ائتلاف عام بالاتجاه المرغوب في الابوان(Sy - 22 وMGW - 7) بينما في الاباء المذكرة اعطى الابوان (MGW - 3 وS - 165) افضل ائتلاف عام بالاتجاه المرغوب للصفات المدروسة بوجود عملية المغنطة وبغيابها للموسمين ، اما التضريبان (M - 17×Zm - 9) و(MGW - 3×MGW - 7) اعطيا افضل ائتلاف خاص بالاتجاه المرغوب للصفات المدروسة بوجود عملية المغنطة وبغيابها للموسمين . كان التباين الوراثي اكبر من التباين البيئي لجميع الصفات والتباين الوراثي المضيف كان اكبر من التباين الوراثي السيادي لاكثر الصفات المدروسة وانعكست هذه النتائج على نسبتي التوريث اذ كانت ضمن الحدود العليا لجميع الصفات تراوحت بين 68.93 و71.57% لصفة المدة من الزراعة الى 50%من النباتات تزهيرا ذكريا بغياب المغنطة وبين 99.86 و99.70% لحاصل النبات بوجود عملية المغنطة للموسمين بالتتابع ،وتراوحت نسبة التوريث بالمعنى الضيق بين 31.04% و72.59% لصفة ارتفاع النبات وعدد الصفوف بالعرنوص بغياب عملية المغنطة بالتتابع للموسم الاول ، وبين 34.94% و75.02%لصفة ارتفاع العرنوص والمدة من الزراعة الى 50%من النباتات تزهيرا انثويا بوجود عملية المغنطة وبغيابها بالتتابع للموسم الثاني ، وكان معدل درجة السيادة اكبر من واحد في اغلب الصفات المدروسة بوجود عملية المغنطة وبغيابها للموسمين مما يشير الى سيطرة السيادة الفائقة للجينات في توريث الصفات المدروسة. وبينت نتائج التجربة المختبرية لتحليل الـDNA للسلالات المدروسة باستعمال مؤشرات الـ RAPD ادت الى ظهور الحزم في 14 بادئ ولم تظهر في البادئB11 ، اذ لم يعثر على اي تتابع مكمل له من الـDNA السلالات المدروسة وبلغ عدد الحزم الكلية 150 حزمة بمعدل 10 حزمة لكل بادئ وبلغ عدد الحزم المتباينة الكلية71 حزمة وبنسبة 47.33% اما الوزن الجزيئي للبادئات المستعملة فتراوح ما بين 65 - 1850bp ، وبلغت اعلى نسبة تشابه وراثي 0.67 بين السلالتين (Sy - 9) و(Sy - 1) بينما بلغت اقل نسبة من التشابه الوراثي 0.33 بين السلالتين (MGW - 3) و(Sy - 22) وان السلالة(MGW - 3) اكثر السلالات تباعدا وراثيا عن السلالات الاخر ، اظهر التحليل العنقودي للسلالات المدروسة باستعمال مؤشرات الـ RAPD واعتمادا على طريقة UPGMA ان السلالات توزعت في مجموعتين رئيستين شملت المجموعة الاولى سلالتين فقط بينما شملت المجموعة الثانية مجموعتين ثانويتين والتي بدورها انقسمت الى مجاميع تحت الثانوية . | A field experiment was Carried out in Al - Musayib project during spring and fall seasons of 2015 and fall of 2016 .The study included eight inbreds line of maize which was (Sy - 9 , MGW - 3 , S - 165 , M - 17 , Zm - 9 , Sy - 22 , MGW - 7 and Sy - 1) and divided into four inbreds as female and four as males the crossing was conducted by factorial mating suggested by Comstock and Robinson (1948,1952) .The grains of parents were planted during spring season 2015 and the crossing between them was conducted and the grains for 16 hybrids were obtained. in fall season in 2015and 2016 the seeds were divided into two parts, the first was exposed to magnetization process of 1500 gauss for aperiod of 30 minutes and the other part without magnetization.F1 and parent seeds were planted by using R.C.B.D Randomized Complete Block design with arrangement of the split - plots including magnetization and non magnetization of the main plots where as the genotypes included sub plots with three replications to estimate hybrid vigor , combining ability , some genetic parameters and the degree of dominance and effect magnetization grains on growth traits and yield component and yield. Laboratory experiment was conducted of certification of grains laboratories office of Agriculture Ministry to study the genetic diversity between the studied inbreds. The results showed a high significant differences between the magnetization and non magnetization process for genotypes and their interaction except the number of ears which were significant for two seasons , Heterosis arranged between negative and positive values, the highest power of negative hybrid reached - 18.08 for the number of rows in hybrid (Zm - 9 × Sy - 9) in the first seasons and - 36.07% for number ears in hybrid (S - 165 × Sy - 1) for second season with out magnetization , where as the hybrid (MGW - 7 × MGW - 3) gave the highest positive hybrid vigor which reached 188.17% , 181.25% for the yield plant with magnetization and with non magnetization for the first season and second season respectively.The female parents showed better general combination for the parents (Sy - 22 , MGW - 7), where as male parents, the parents (MGW - 3 , S - 165) gave better general combination ability for studied traits with and with out magnetization for the two seasons. The hybrids (MGW - 3 × MGW - 7) and(M - 17 × Zm - 9) gave better special combination ability for the studied traits with and with out magnetization for the two seasons . The genetic variance was greater than the environmental variance for all traits, while additive genetic variance was greater than dominance genetic variance for most studied traits , The results reflected at the heritability ratio which arranged for all traits between 68.93% , 71.57% for period of planting to 50% tasseling non magnetization and 99.86% , 99.70% for yield plant with magnetization and for the two seasons respectively . The narrow sense heritability ranged from 31.04% , 72.59% for the plant height and the number of rows per ear with out of magnetization for the first season and ranged form 34.94% , 75.02% for ear height and the silking to50% with magnetization and non magnetization respectively for the second season. The average of dominance degree was greater than one in most studied traits the with magnetization and non magnetization for the two seasons which indicating to the control of over dominance of the genes in the inheritance of the studied traits .The results of laboratory experiment showed for analysing of the DNA for studied inbred by using RAPD indicators which casused the emergence of fragments in 14 where as didn,t appear in B11 primers, no finding for any complementary sequence of its from DNA for studied inbred, the total number of these fragment were 150 with average of 10 fragment for each primer , the number of polymorphic fragments were 68 with ratio of 47.33% , the molecular weight of the primers used ranged from 65 - 1850bp, the highest genetic similarity reached to 0.67 between inbred (Sy - 9) and (Sy - 1) where as the lowest portion of the genetic similarity was 0.33 between inbred (MGW - 3) and (Sy - 22) ,the inbred (MGW - 3) was more diversity from other breeds,The cluster analysis for studied inbred by using the RAPD indicators by depending on UPGMA method showed that the inbreds were distributed into two main groups, the first group included two, secondary group which in turn splited to groups under secondary

تاثير التقنين الغذائي الكي واضافة المعزز الحيوي وحامض البروبيونك في العلائق على الاداء الانتاجي والفسلجي لفروج اللحم == Effect of Quantitative feed restriction with Addition Probiotic and Propionic Acid on Productive Performance and Some Physiological Characters of Broiler Chicks

Author name: الفريد سولاقه كرومي حنا

Supervisor name: رياض كاظم موسى | عبد الله عبد المنعم محمد

General topic: Agricultural sciences

Specific topic: Zoology

Degree: Doctorate

University: University Of Basrah - Faculty Of Agriculture

Language: Arabic

University location: Basrah

First pages:

Abstract: This study was conducted at poultry farm, Animal Resources Department, College of Agriculture University of Basrah from 4/4/2010 to 15/5/2010 for the first experiment and from 8/112010 to 19/12/2010 for the second experiment. The aim of this study was to investigate the effect of early (8 - 21) and late (21 - 34) day of feed restriction by partial 20% with adding IRAQI probiotic at level 0.3% for (first experiment) and propionic acid at level 0.3% for the (second experiment) in productive performance and some physiological of blood and small intestine. One day of 270 unsexed chicks of Ross 308 strain were used in the study. The chicks were randomly distributed into 6 treatments with three replicates. Each replicate contain 15 chicks. Results of first experiment indicated that : 1 - There was a significant (p<0.05) increase in control treatment with IRAQI probiotic on live body weight and body weight gain. While, there was a significant (p<0.05) decrease in the late feed restriction treatment with, or not adding probiotic.2 - There was a significant (p<0.05) increase for the control treatment with, or not adding probiotic in feed consumption.3 - There was a significant (p<0.05) improvement in feed conversion ratio of early and late feed restriction with, or not adding probiotic.4 - Late feed restriction and early feed restriction with probiotic cause significant (p<0.05) decrease in mortality comparing with control treatment.]b[5 - There was a significant (p<0.05) decrease in abdominal fat for the treatments early and late feed restriction with, or not addition probiotic as compared with the control treatment.6 - There was significant (p<0.05) reduction in Intestine PH for the treatment adding probiotic, but there was no differences between the treatments in Ceca PH.7 - There was a significant (p<0.05) decrease in total protein, albumin, cholesterol and glucose in blood serum for the chicken with early feed restriction (not adding probiotic), and there was significant (p<0.05) increase GOT and GPT levels in the same treatment at the age of 21 days, Addition of IRAQI prpbiotic showed a significant (p<0.05) reduction of serum cholesterol, GOT and GPT as compared with control treatment.8 - There was a significant (p<0.05) decrease in cholesterol, GOT and GPT by the addition IRAQI probiotic compared with control treatment at 42 days.Results of second experiment indicated that : 1 - There was a significant (p<0.05) decrease in the treatments (late feed restriction, early and late feed restriction with, or not adding propionic acid) on live body weight and body weight gain.2 - There was significant (p<0.05) decrease in feed consumption for the treatment early and late feed restriction with, or not addition propionic acid. 3 - There was significant (p<0.05) decrease in feed conversion for the treatment early and late feed restriction with addition propionic acid compared with the other treatments.4 - Late, early feed restriction with or not addition propionic acid cause significant (p<0.05) decrease in mortality compared with control treatment, and there was significant (p<0.05) reduction in production index for the treatment early and late feed restriction with addition propionic acid as compared with the other treatments.5 - There was a significant (p<0.05) decrease in abdominal fat for the treatments early and late feed restriction with, or not addition propionic acid compared with the control treatments.6 - There was a significant (p<0.05) decrease in total protein, globulin, cholesterol and glucose in blood serum for early feed restriction with, or not adding propionic acid, but there was significant (p<0.05) increase at the level of GOT and GPT in the same treatment compared with the other treatments at the age of 21 days.7 - Serum cholesterol showed significant (p<0.05) reduction in the late feed restriction with, or not adding propionic acid compared with the other treatments at the age of 42 days.

عزل وتشخيص البكتريا المحللة للفينول من المياه والعوامل البيئية المؤثرة عليهوادراسة بعض العناصر المعدنية في مياه الفضلة الصناعية == Isolation and Identification of Phenol Degradable Bacteria from Water and Studying The Environmental Factors Affecting them with some Mineral Elements in Industrial Wastewater

Author name: سـوزان كامران حسن

Supervisor name: حسن رحيـم الشريفـي

General topic: Agricultural sciences

Specific topic: Food

Degree: Doctorate

University: University of Baghdad

Language: Arabic

University location: Baghdad

First pages:

Abstract: تم الحصول على ست عزلات بكتيرية محللة للفينول من مياه الفضلة الصناعية لمصفى الدورة وشخصت باستخدام جهاز Vitek 2 System الخاص بتشخيص البكتريا، كانت 4 من هذه العزلات Pseudomonas fluorescens وعزلة واحدة Citrobacter freundii واخرى Serratia liquefaciens واختيرت من بينها العزلة الاكثر قابلية على تحليل الفينول وهي العزلة Pseudomonas fluorescens SK اذ بلغت اعدادها 262 × 105 و.ت.م/مل عند تنميتها على الفينول مصدرا وحيدا للكربون.تم تاكيد تشخيص العزلة المنتخبة للدراسة SK Pseudomonas fluorescens باستخدام تقنية التفاعل المتسلسل PCR ودرس تسلسل القواعد النتروجينية في الولايات المتحدة الامريكية وتم الاستدلال على ان هذا التسلسل يعود الى Pseudomonas fluorescens بعد مطابقة التسلسل الناتج مع التسلسلات الموجودة في بنك الجينات في NCBI (National Center for Biotechnology Information, USA ). اظهرت نتائج التضخيم والترحيل الكهربائي ان العزلة المنتخبة للدراسة Pseudomonas fluorescens SK خالية من انزيم Phenol hydroxylase الذي اختير بالتشخيص كاحد الانزيمات المهمة لتحليل الفينولات.درست الظروف البيئية المؤثرة في نمو العزلة البكتيرية المنتخبة (توفر الاوكسجين ومدة الحضن والدالة الحامضية ودرجة الحرارة وتركيب الوسط) وقد وجد من النتائج ان للتهوية تاثيرا جيدا في زيادة النمو. فقد اظهرت المزارع المتحركة اعلى نموا للخلايا الحية 187×410 و.ت.م./مل وان معظم الزيادة في الكتلة الحيوية حصلت بعد 48 ساعة من الحضن اذ بلغت الاعداد 275 x 10 4 و.ت.م./مل والدالة الحامضية 7 كانت هي الافضل اذ اعطت اعلى نمو للعزلة اذ بلغ النمو 232 ×10 4 و.ت.م./مل، اما درجة الحرارة فكانت 25 ˚م اذ سجل عندها اعلى نمو للعزلة والذي بلغ 271×410 و.ت.م./مل .و عند استخدام الوسط التالي الوسط المعدني الحاوي على (100ملغم/لتر فينول مضافا اليه الكلوكوز مصدرا كاربونيا ومستخلص الخميرة مصدرا نيتروجينيا) فقد اعطى اعلى نمو للخلايا بلغ 290 × 104 و.ت.م. /مل.درست قدرة العزلة البكتيرية Pseudomonas fluorescens SK على تحليل تراكيز متعددة من الفينول ، واظهرت النتائج ان اعلى نمو للعزلة P.fluorescens SK كان عند تركيز 250 ملغم فينول/لتر اذ بلغ عدد الخلايا 285 ×10 5 و.ت.م. /مل.اجريت دراسة الفعل التازري لعمل بكترياSK P. fluorescensمع البروتوزوا في تحليل الفينول كلا على حده وخليطا مع بعضها، ومن خلال النتائج تبين ان المزارع المختلطة (البكتريواالبروتوزوا) معا افضل في استهلاك المخلفات النفطية من المزارع المفردة (البكتريا) فقط اذ وجد ان تركيز الفينول المتبقي بعد تحلله بفعل خليط (البكتريواالبروتوزوا) معا يبلغ 2.12 ملغم/لتر، اما تركيز الفينول االمتبقي بعد تحلله من العزلة البكتيرية P.fluorescens SK فكانت 2.41 ملغم/لتر ، وبفعل البروتوزوا بلغ 2.07 ملغم/لتر مقارنة مع انموذج السيطرة الذي بلغ 3.1 ملغم /لتر. تم تقدير تركيز الفينول بتقنية كروماتوغرافي الغاز السائل GLC لنماذج المياه المعقمة المعباة فوجد انه يبلغ 1.199 ملغم/لتر ولمياه الاسالة تراوح بين 1.896 - 9.721 ملغم/لتر ولنماذج مياه الصرف الصناعي(المعالجة وغير المعالجة بايولوجيا) لمصفى الدورة بلغ 0، 187 ملغم/لتر على التوالي ووجد خلو مياه نهر دجلة من الفينول تماما. اجريت دراسة تقدير العناصر الثقيلة المتمثلة بـPb, Cd, Fe ,Cr, Ni, Cu الموجودة في مياه الصرف الصناعي لمصفى الدورة (قبل وبعد المعالجة البايولوجية ) ومياه نهر دجلة وقد اظهرت النتائج خلو الرصاص في نماذج المياه التي جمعت من الحوض قبل المعالجة البايولوجية وبعدها لمصفى الدورة ،اما تركيز الرصاص في نماذج المياه التي جمعت من نهر دجلة فقد بلغ 0.0218 ملغم/لتر.بينما كان تركيز الكاديميوم في مياه نهر دجلة عاليا اذ بلغ 0.0075 ملغم/لتر وفي نماذج المياه التي جمعت من الحوض قبل المعالجة البايولوجية بلغ 0.0086 ملغم/لتر وشهد انخفضا الى 0.0038 ملغم/لتر بعد المعالجة البايولوجية .في حين كان الحديد في نماذج مياه الصرف الصناعي قبل المعالجة البايولوجية عاليا بتركيز مقداره 3.2208 ملغم/لتر وحدث فيه انخفاض حاد في نماذج مياه الصرف الصناعي بعد المعالجة البايولوجية فقد بلغ 0.0368 ملغم/لتر وكان تركيز الحديد في نهر دجلة 0.0053 ملغم/لتر. لم يتاثر تركـيز الكـروم في نماذج المـياه جميعـها التي تم جمعها من الحوض قبل المعالجة البايولوجية وبعدها اذ بلغ 0.0095 ملغم/لتر، اما في مياه نهر دجلة فقد بلغ 0.0072 ملغم/لتر.كانت نتائج تراكيز النيكل لنماذج المياه التي جمعت من الحوض قبل المعالجة البايولوجية 0.0215 ملغم/لتر ولوحظ اختفائه في نماذج المياه بعد المعالجة البايولوجية تماما وكان مياه نهر دجلة خالية منه ايضا.كان تركيز النحاس في نماذج مياه الصرف الصناعي قبل المعالجة البايولوجية 0.0131 ملغم/لتر وانخفض بعد المعالجة البايولوجية وفي مياه نهر دجلة الى 0.0016 و0.0005 ملغم/لتر على التوالي. اجريت القياسات الفيزيائية والكيميائية لعينات مياه الصرف الصناعي لمصفى الدورة قبل المعالجة البايولوجية وبعدها، لمعرفة العوامل البيئية المحيطة مثل : درجة الحرارة ، والدالة الحامضية في الحقل ، والمتطلب البايولوجي للاوكسجينBiologycal Oxygen Demand (BOD5) ، والمتطلب الكيميائي للاوكسجين((COD Chemical Oxygen Demand، المواد الصلبة الذائبة الكلية(TDS) والمواد الصلبة العالقة الكلية (TSS) والعسرة الكلية والكبريتات والقاعدية الكلية والفوسفات Phosphate ، الكلوريدات Chloride وثاني اوكسيد الكاربون والمركبات الهيدروكاربونية المقاسة في المختبر وكانت القيم على النحو الاتي (32 ،30˚م) ،(6 - 8)،(43 ، 10 ملغم/لتر) ،(520 ، 60 ملغم/لتر) ، (1156 ، 1121 ملغم/لتر) ، (320 ، 43 ملغم/لتر) ، (496 ،500 ملغم/لتر) ، (0.371 ،0.045 ملغم/لتر) ,( 168 ،155 ملغم/لتر) ،(1.6 ،0.2 ملغم/لتر) ،(200 ،188 ملغم/لتر) ،(9.68 ،2.64 ملغم/لتر) ،(28.2 ، 1 ملغم/لتر ) وعلى التوالي. | Six isolates were isolated from industrial wastewater from Al - Dorah oil refiner and they were identified by the Vitek identification system. Four of these were Pseudomonas fluorescensand the others were Citrobacter freundii and Serratia liquefaciens. Isolate Pseudomonas fluorescens SK was chosen as the best one which can degrade phenol and its total number was262x105c.f.u.\ml.Identification of Pseudomonas fluorescens was reassured by PCR technique, also nitrogen base sequence was studied in the USA, and it was confirmed that it was belonged to Pseudomonas fluorescensas it was matched with the sequence in National Center for Biotechnology information, USA. Pseudomonas fluorescens SK has not phenol hydroxylase as it was proved by PCR and electrophoresis . Best Environmental conditions for the growth of the selected isolate (Oxygen, incubation time ,pH ,temperature and the medium) were : shaking culture 48 h ,pH 7 , 25˚C as the total bacterial numbers were : 187x104 , 275x104 , 232x104 and 271x104c.f.u.\ml. The best culture media formula was mineral media, which consists of 100 mg\L phenol, plus glucose and Yeast Extract. Highest phenol concentration which Ps. Fluorescens SK can degrade and growin was 250 mg\L as the total number was 285x105c.f.u.\ml . Phenol concentration was determined by GLC technique in bottled water was 1.99 mg\L and tap water was 1.891 - 9.721 mg\L and in untreated and biological treated industrial wastewater was ND and 12.7 mg\L, but it wasn’t detected in water samples taken from Tigris river. Synergistic action of Ps. Fluorescens SK plus a protozoa gave better results to degrade phenol as the remaining phenol was 2.12 mg\L whereas. The culture of Ps. Fluorescens SK alone gave 2.41mg\L ,and the protozoa alone 2.07 mg\L comparing to control one it was 3.1 mg\L. Heavy metals Pb, Cd, Fe, Cr, Ni and Cu were determined in untreated and biologically treated samples of Al - Dorah oil refiner industrial water also in the Tigris River samples no lead was detected in the refiner samples but it was found in Tigris samples 0.0218 mg\L. Cadmium in Tigris sample was 0.0075 mg\L and in the refiner water was 0.0086 mg\L and 0.0038 mg\L in the untreated one. The highest concentration of Iron in the refiner water before treated was detected 3.2208 mg\L and it decreased to 0.0368 after treatment and in the River samples was 0.0053 mg\L. Chromiumconcentration was 0.0095 mg\L in the refiner water before and after biological treatment, and in the River samples was 0.0072 mg\L. Nickel concentration in the refiner water was 0.0215 mg\L and no Nickel was detected after treatment, also it was not detected in the River samples. The highest concentration of Copper in the refiner water before treatment was detected 0.0131 mg\L and it was decreased to 0.0016 mg\L after treatment and in the River samples was 0.0005 mg\L. Physical and chemical analysis for the refiner wastewater before and after biological treatments was carried out for temperature, pH ,Biological oxygen demand (BOD5), Chemical oxygen demand (COD),Total dissolved solids (TDS),Total suspended solid (TSS), Total Hardness , Sulfate , Phosphate, CO2 , Total Alkalinity , Chloride and total Hydrocarbon compound and they were (32 ,30˚ C) , (6 - 8), ( 43 ,10 mg\L ) , (520 ,60 mg\L), (1156 , 1121 mg\L) ,(320 ,43 mg\L), (496 , 500 mg\L ),(0.371 , 0.045mg\L) ,(168 ,155 mg\L),(1.6 , 0.2 mg\L),(200 ,188 mg\L) ,(9.68, 2.64 mg\L),( 28.2 , 1 mg\L) Respectively.

Author name: احمد محمدحسين حسن

Supervisor name: مجيد متعب ديوان | عدنان نعمة الاسدي

General topic: Agricultural sciences

Specific topic: Plant - Prevention

Degree: Doctorate

University: University of Kufa - Faculty Of Agriculture - Department Of Plant Protection

Language: Arabic

University location: Najaf

First pages:

Abstract: استهدفت هذه الدراسة تاثير اضافة الكتلة الحيوية للفطر المحاري Pleurotus ostreatus الى العليقة مع اضافة راشح الوسط الزرعي للفطر لماء الشرب في الاداء الانتاجي والفسلجي لفروج اللحم , اذ تم زراعة الفطر المذكور بالمختبر على تبن الحنطة وحضن بدرجة حرارة 25 + 2 م لمدة 21 يوما , اما التجربة الحقلية فقد نفذت في حقل الطيور الداجنة التابع لقسم الثروة الحيوانية ، كلية الزراعة / جامعة الكوفة , ونفذت خلال المدة من 26/10/2014 ولغاية 1/12/2014 , استخدم في هذه التجربة 360 فرخ فروج لحم غير مجنس من السلالة التجارية روز (Rose - 308 ) . ربيت الافراخ على فرشة ارضية ، واعطيت معاملات التجربة الى الافراخ من عمر 7 يوم الى عمر 35 يوم ، واظهرت النتائج تقارب محتويات كل من الجسم الثمري والكتلة الحيوية للفطر المحاري P. ostreatus من محتواها من بعض العناصر الغذائية مع تفوق الكتلة الحيوية على الجسم الثمري بعدد الايونات الذائبة لكل من عنصر Ca++ , Mg++ والنسبة المئوية للنتروجين (N) , كموااظهرت النتائج انه بزيادة فترات التحضين للفطر المحاري على انواع التبن تزاد معها النسبة المئوية للبروتين الخام معنويا, وحصلت اعلى زيادة في نسبة البروتين الخام مع تبن الحنطة , وتلاها تبن الشعير ثم الشلب . بينت ايضا نتائج الدراسة قدرة تنافسية عالية للفطر P. ostreatus ضد عدد من الفطريات الملوثة للعليقة , حيث غطى الفطر المذكور كامل مساحة الطبق الذي سبق وزرع ببعض الفطريات دون السماح لها بالنمو . لوحظ تفوق معنوي لمعاملتي اضافة راشح الفطر بنسبة 100 مل/لتر ماء شرب واضافة الكتلة الحيوية للفطر بنسبة 50 غم/كغم عليقة وراشحه بنسبة 50 مل/لتر ماء شرب في معدل وزن الجسم الاسبوعي والزيادة الوزنية الاسبوعية (غم) لفروج اللحم على معاملة المقارنة وباقي المعاملات . وايضا كان هناك تحسن معنوي لمعامل التحويل الغذائي بين طيور معاملات اضافة راشح الفطر المذكور بالنسبة 100 مل/لتر ماء الشرب ومعاملة اضافة كتلته الحيوية بنسبة 50 غم/كغم عليقة مع 50 مل/ لتر من راشح الفطر والذي بلغ 1.38 و1.47 غم علف/غم زيادة وزنية حسب الترتيب مقارنة بمعاملة السيطرة التي بلغ فيها معامل التحويل الغذائي 1.82 غم علف/غم زيادة وزنية . بينت النتائج ان اضافة الكتلة الحيوية للفطر P. ostreatus الى العليقة وراشحه الى ماء الشرب قد حسن معنويا او حسابيا من حجم خلايا الدم المرصوصة لجميع المعاملات مقارنة مع معاملة السيطرة . وتبين ايضا ان اضافة الكتلة الحيوية للفطر P. ostreatus الى العليقة وراشحه الى ماء الشرب قد خفض في اعداد البكتريا الهوائية الكلية وبكتريا القولون في الامعاء الدقيقة للافراخ معنويا كلمزاادت النسبة المضافة . كذلك لوحظ من خلال دراسة تكاليف العليقة للمعاملات المختلفة ان معاملتي اضافة الكتلة الحيوية للفطر بنسبتي 50 و100 غم/كغم عليقة قد اعطيتا اعلى معدل في استهلاك العليقة بلغ 3198.92 و3305.82غم حسب الترتيب بكلفة 2314.1 و2319.2 دينار لكل منهما حسب الترتيب . وان الربح المتاتي من هاتين المعاملتين هاتين مقارب الى الربح المتاتي من معاملة المقارنة الذي بلغ 3427.2 و3483.1و 3595.8 دينار لكل منهوا حسب الترتيب . واظهرت النتائج ايضا ان معاملة اضافة راشح الفطر بتركيز 100 مل/لتر ماء شرب قد اعطت اعلى صافي ربح بلغ 5157.0 دينار , تلتها معاملة اضافة راشح الفطر بنسبة 50 غم/كغم عليقة و50 مل/لتر ماء شرب بلغت 4827.4 دينار فيما بلغ صافي الربح في معاملة المقارنة 3595.8 دينار . | The research aims to the effect of adding biomass of oyster mushroom Pleurotus ostreatus to the Diet, and exudates to drinking water and the mixture is synergistic to them in productive performance and Physiology for broiler chickens, as was the cultivation of mushrooms mentioned in the laboratory on the straw of wheat and lap at a temperature of 25 +2 ° C for 21 days, This study was conducted in the Poultry farm, Animal resources department, Agriculture Faculty / University of Kufa, and Perform the field experience special for this study during the period from 26/10/2014 until 01/12/2014 . was used in this experiment 360 broiler chickens t is naturalized commercial strain Rose (Rose - 308). nurtured chicks mattress on the floor, and were given experimental treatments to chicks from age 7 to age 35 on the day , We reached the end of the search to several results, including : The convergence of the contents of each of the fruiting body and biomass of oyster mushroom P. ostreatus of some nutrients with superiority biomass fruiting body on a number of dissolved ions for each element of Ca ++, Mg ++ and the percentage of nitrogen (N) . Observed a significant superiority in treatment of Add exudates mushroom at 100 ml / liter of drinking water and the addition of biomass of the fungus by 50 g / kg feed and exudates at 50 ml / liter of drinking water in the rate of body weekly weight and increase the weight weekly (g) of chicken meat to the treatment of the comparison and the rest of the treatment . While my treatment achieved Add biomass mushroom at 50 and 100 g / kg feed a significant superiority for the treatment of control, reaching the two feed weekly consumption rate of 611.45 and 658.67 gm. The rest of the treatment did not show significant differences between them and the control treatment. also there was a significant improvement in the coefficient of feed conversion between birds treatment Add exudates in the proportions of 25, 50 and 100 ml / liter of drinking water and the treatment of adding bio mass by 50 g / kg feed with 50 ml / l of exudates , which stood at 1.74 and 1.65 and 1.38 and 1.47 g feed / g increase in the order by weight compared to the control treatment in which the feed conversion factor of 1.82 g feed / g increase grains. The results showed that the addition of the biomass of the fungus P. ostreatus to feed and leaking into drinking water has improved significantly or mathematically stacked size of blood cells of all treatment, compared with the control treatment. Also shows that adding biomass of the fungus P. ostreatus to feed and leaking into drinking water was reduced in the preparation of the total aerobic and coliform bacteria in the small intestine of chicks was significantly higher the percentage added. Also it observed by studying feed costs of the various treatment that my treatment Add biomass mushroom in 50 and 100 g / kg feed may highest rate in the feed consumption stood at 3305.82 and 3198.92 g in the order at a cost of 2314.1 and 2319.2 dinars each in the order. And that the profit derived from these two treatments were not significantly different from the profit derived from the treatment of comparison, reaching 3427.2 and 3483.1 and 3595.8 dinars for each of them and by the order. It also results showed that the treatment Add exudates mushroom concentration of 100 ml / liter of drinking water has given the highest net profit of 5157.0 dinars, followed by treatment Add exudates mushroom by 50 g / kg feed and 50 ml / liter of drinking water amounted to 4827.4 dinars, while net profit in comparison 3595.8 dinars