Share
تاثير الباكلوبيوترازول والكازين في تكوين الاجنة الخضرية وانباتها لنخلة التمر Phoenix dactylifara L صنف نيرسي
Author name:
زياد طارق صافي عبد العلي
General topic:
Agricultural sciences
Specific topic:
Plant
Degree:
Doctorate
University:
University Of Basrah - Faculty Of Agriculture - Department Of Soil Sciences And Water Resources
Language:
Arabic
University location:
Basrah
First pages:
31T1706 - p.pdf
Abstract:
Present study was conducted in the Plant Tissue Culture Laboratory, Agriculture Collage, Basrah University, Basrah , Iraq, in the period 2012 - 2014. The objective of this study was to evaluate the effect of different concentrations of growth retardant (paclobutrazol) and casein hydrolysate on somatic embryogenesis formation and germination in date palm (Phoenix dactylifera L.) cultured in vitro. The shoot tip explants were excised from offshoots that approximately 3 - 4 years old. Shoot tip explants then cut it in four segments and each segment cultured on Murashige and Skoog (MS) basal medium containing different concentrations of paclobutrazoal (0, 0.3, 0.5, 0.7, 0.9 and 1.0 mg/L) or casein hydrolysate (0, 200, 400, 600, 800 and 1000 mg/L).Results obtained in this study can be summarized to : 1 - Study showed that the MS medium containing pacloputrazol (1.0 mg/L) or casein hydrolysate (1000 mg/L) increased in fresh and dry weight of primary callus and embryogenic callus comparing to control treatment.2 - Primary and embryogenic callus cultured on MS medium fortified with 1.0 mg/L paclobutrazol for a long period showed superiority in fresh and dry weight compared with other treatments and control.3 - The period need it for somatic embryo formation was decreased to (17 and 20.33 days) when the embryogenic callus with 0.5 and 0.7 mg/L of paclobutrazol respectively. Results also revealed that the numbers of white and big spherical somatic embryos were appeared when high concentrations of paclobutrazol 1mg/L and casein hydrolysate (1000 mg/L) have been used.4 - The rate of somatic embryos length were increased when the concentration of casein hydrolysate increased to 1000 mg/L. In contrast, length rate of somatic embryos were decreased when treated with paclobutrazol.5 - High fresh and dry weight of somatic embryo were recorded on MS medium containing (0.9 and 1 mg/L) of paclobutrazol.6 - The high germination rate of somatic embryos was obtained on MS medium containing 0.3 and 0.5 mg/L paclobutrazol or MS medium enriched with 800 and 1000 mg/L in short time in comparing with other treatments.7 - Among the different concentrations of paclobutrazol tested, 0.3 and 0.5 mg/L were the most effective concentrations for increase the length of leaf and primary roots, but the 0.7mg/L and 0.9 led to increase root numbers only, whereas the high concentration of paclobutrazol 1.0mg/L increased the length of leaf and decreased the rate of roots. In contrast the high concentrations of casein hydrolysate (800 and 1000 mg/L) affected positively on leaf and root length.8 - Embryogenic callus tissue subjected to 1.0 mg/L paclobutrazol, containing high carbohydrate, whereas, embryogenic callus treated with 0.5 and 0.7 of paclobutrazol containing high protein. In contrast, embryogenic callus tissue treated with different concentrations of casein hydrolysate containing high carbohydrate and protein.9 - Histological study revealed that the high concentration of paclobutrazol reduced the size of cells in compare with control treatment.
