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تاثير الماء المعالج مغناطيسيا في بعض الصفات المظهرية والكيموحيوية في عقل نبات الماش (Phaseolus aureus Roxb.) == Effect of Magnetically Treated Water On Some Morphological And Biochemical Properties of Mung Bean (Phaseolus Aureus Roxb.) Cuttings

Author name: عامر راضي عبد الحسين جبار
Supervisor name: بشير عبد الحمزة محمد العلواني
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Babylon - College Of Science - Department Of Biology
Language: Arabic
University location: Babylon
First pages:
Abstract: This study was conducted in order to investigate the role of magnetically water in the rooting of fresh and aged (phaseolus aureus roxb.) cuttings, and the impact of treated water in some morphological criteria and biochemical that interfere in the induction of rooting in cuttings and fall clearly when delayed induced auxin treatment in aged cuttings. Study was conducted in the laboratories of the Department of biology at the University of Babylon, for the period 2012 - 2014 was the use of magnetic therapy devices (Magnitron) of water with three gauss 1000, 2000.3000 so as to provide a magnetic flow to distilled water and river water and used as well as the complete randomized design and analyzed the results statistically significant and valuable knowledge LSD to extract the significantly differences between the treatments and the results were as follows : Physiological parts1. Increase the number of roots significantly in cuttings when treat for 24 h.with auxin dissolved with distilled water processor in 1000 gauss amounted to 77.75 root / cutting.2. The results showed that the roots rate increased significantly in the treatment of fresh cuttings treated in distilled water processor 1000 gauss gave 64 root / cutting. As well as the rest treatments affect in rooting increase as given (58, 52.25) root / cutting for each of the treatment 2000.3000 gauss, respectively.3. The treatment of river water as well as magnetically influenced in increasing the number of roots significantly in three processes and the highest being in the 1000 gauss(84) root / cutting.4. The results showed that distilled water processor magnetic effect is significant in the processes that occur during ageing of the cuttings, which led to a reduction in rooting rate in aged cuttings, as it was observed that the treatment of the cuttings for 72 - hour in 1000 gauss is the best of the rest of the treatments amounted to 48 root / cutting. As the impact of river water processor magnetically significantly increase the number of roots in the aged cuttings as treatment given to 1000 gauss higher values 70 root / cuting.Biochemical parts.1. The results showed that chlorophyll content increased significantly when using the river water wizard three intensities (18.09, 17.43, 16.99) (spad) intensities of 1000, 2000.3000 gauss respectively.other each of distilled water and river water processor magnetically in 1000 gauss increase in chlorophyll content to 16.07, 18.57 (spad) respectively in aged cuttings.2. It was observed that the treatment of auxin dissolved in three intensities impacted significantly on the effectiveness of antioxidants defense system and the highest being at the treatment of the cuttings in auxin dissolved water magnetically processor in 1000 gauss, as was the effectiveness of the enzyme SOD 1.85 unit in the effectiveness of the enzyme CAT reached 15.30 unit and increased GSH content to 0.914 mg / g when compared with the control treatment.3. influenced the treatment of fresh cuttings in distilled water magnetically processor with three intensities are equally matched in the effectiveness of SOD and CAT, and the highest being at 1000 gauss magnetic intensity as it affected the effectiveness of the CAT to 31.62 units. Magnetic intensity also affected the same increase in GSH content reached significantly to 0.710 mg / g.4. The results showed the treatment of fresh cuttings river water magnetically wizard in three intensities increase in the effectiveness of the enzyme SOD and CAT, and the highest being at the treatment of 1000 gauss, amounting to 4.168, 45.9 U for both enzymes, respectively, as GSH content increased significantly, reaching 0.810 mg / g in the treatment of the fresh cuttings in 1000 gauss.5. I have not noticed any significant effect of distilled water processor magnetically as well as river water in the MDA content.6. The impact of distilled water processor 1000 gauss significantly in increasing the effectiveness of the enzyme SOD and CAT in aged cuttings, amounting to 2.88, 42.84 units respectively, as GSH content increased to 0.785 mg / g when the treatment of tresh cuttings in distilled water processor with 1000 gauss and also affected the rest of the intensities increase in GSH content in aged cuttings.7. increased the effectiveness of enzyme SOD and CAT when treated of aged cuttings in river water processor magnetically 1000 gauss, amounting to 2.9, 51.2 units respectively, as GSHwas significant increase in content to 0.854 mg / g when treatment aged cuttings in river water processor 1000 gauss and also affected the rest of the intensities increase in GSH content.8. The use of treated water magnetically 1000 gauss significantly effect in reducing the MDA content in aged cuttings 0.293 mg/g fresh weight.

دراسة وراثية وبكتريولوجية على بكتريا تثبيت النيتروجين التكافلية Ensifer meliloti == Bacteriological And Genetic Study On Symbiotic Nitrogen Fixing Bacteria Ensifer Meliloti

Author name: محمد زهير ناجي المرعب
Supervisor name: حسن فاضل ناجي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
University: University of Babylon - College Of Science - Department Of Biology
Language: Arabic
University location: Babylon
First pages:
Abstract: عزلت عشر عزلات من بكتريا Ensifer meliloti من العقد الجذرية لنبات الجت Medicago sativa L. بعد ان جمعت من مناطق مختلفة من محافظة بابل خلال المدة من شهر تشرين الثاني/2012 لغاية شهر شباط/2013. شخصت العزلات جزيئيا بتقنية تفاعل البلمرة السلسلي فضلا عن طرق ال | Ten isolates of Ensifer meliloti were isolated from root nodules of Medicago sativa L. collected from different areas of Babylon province during a period from November - 2012 to February - 2013. The isolates were molecular identified using polymerase chain reaction technique, in addition to traditional methods (morphological and biochemical). All isolates were harboured nodbox and mucR genes and produced cyclic ? - (1, 2) glucans, cellulose fibers and lipopolysaccharide.The ability of isolates to grow at different degrees of pH, temperatures and different concentrations of sodium chloride were tested. It was found that the optimum pH for growing of Em1, Em3, Em4, Em6 and Em8 isolates was pH7, while for the isolates Em2, Em5, Em7, Em9 and Em10 was pH8. The optimum temperature for growing of Em1, Em3, Em5, Em7, Em8 and Em9 isolates was 30?C, while the optimal growth of Em2, Em 4 and Em6 isolates was 35?C, Em10 isolate showed significantly extremism to grow at temperture reached to 45?C. The optimal percentages of NaCl for growing of Em1, Em3, Em4, Em5, Em6, Em8 and Em9 isolates were 2%, for Em7 isolate was 3% and for Em2 and Em10 isolates were 4%. The effect of different degrees of pH on symbiotic properties was studied. Em1 and Em8 isolates that grew at pH6.5 ; all isolates that grew at pH 7, pH 7.5 and pH 8 were able to infect the root hairs of alfalfa plants. The isolates that grew at pH6.5 (except Em1 and Em8 isolates) and all isolates that grew at pH8.5 were failed to infect the root hairs and did not formed nodules and resembled to the control plants (without inoculum).Different concentrations of NaCl on symbiosis was studied, it was found that all isolates that grew at 2% and Em2 and Em10 isolates that grew at 3% formed nodule, while Em1, Em3, Em4, Em5, Em6, Em7, Em8 and Em9 isolates that grew at 3% and all isolates that grew at 4%, 5% and 6% did not elicited nodules on the roots of alfalfa plant. Light microscopic observations of root hairs inoculated with Ensifer meliloti isolates showed the root hair deformations like curling, waviness, swelling and formation of shepherd`s crook and infection thread. These consequent changes lead to emerge the mature nodules having five distinct zones viz., distal meristematic, infection, inter, nitrogen - fixing and proximal senescence zones.The gel electrophoresis results of plasmid DNA of isolates showed that these isolates harboured two megaplasmids. When the isolates exposed to extreme factors like pH, temperture and NaCl, it was found that the isolates lost these plasmids and its ability to elicite the nodules and failed to fix the nitrogen. This study showed that the nitrogen fixing bacteria had essential role to support the plant growth and increase their productivity, and recommended to use the isolate Em10 as example for biofertilizers and the best alternative to the chemical fertilizers

دراسة انتشار الحيوانات الابتدائية الرئيسية المسببة للاسهال بين المرضى باستخدام الطرائق المجهرية والجزيئية في محافظة بابل == Prevalence Study For Main Protozoa Diarrheal Agents Among Patients By Using Microscopically And Molecular Methods In Babylon Province

Author name: ميس كاظم عليوي
Supervisor name: احمد خضير عبيس الحميري
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Babylon - College Of Science For Girls - Department Of Biology
Language: English
University location: Babylon
First pages:
Abstract: اجريت الدراسة الحالية خلال المدة من تشرين الاول 2014 الى شهر شباط 2015 حيث تضمنت هذه الدراسة فحص 987 عينة براز (اطفال وبالغين, ذكورو اناث) بطريقة الفحص المجهري المباشر و96 عينة موجبة فحصت عن طريق تقنية تفاعل سلسلة متعدد البلمرة. للمرضى المصابين بالاسهال و| The current study during period was conducted from October 2014 till February 2015 examination of 987 stool samples for direct smear method (Lugol's Iodine, Normal Saline (0.9%) for detection of the following parasites G.lamblia, E.histolytica while using floatation methods and Ziehl - Neelsen method (Malachite green) for detecting the Cryptosporidium spp. by using light microscope) and 96 positive samples from these samples examined by polymerase chain reaction technique PCR. For patients infected with diarrhea (children and adults, male and female) who attended to Babylon maternity and children hospital and specialized Marjan Hospital for Internal and Cardiac Diseases in the Babylon province as well as primary health care and private clinics. The age ranges from(Less than one year - 31and more).The current study showed the rate of infection with parasites that causative of diarrhea47.3% (E.histolytica, G.lamblia and Cryptosporidium spp.) was 26.4%, 17.9% and 3.7%, respectively.They were examined by a direct smear method to detect the trophozoites, cyst and oocyst phases of these parasites. The highest rate of infection by microscopic examination was in the rural area 67.2% in comparing with city that was 32.9%. Also the highest rate of infection among males was 51.5% in comparing with females 41.2%. It has been recorded that the higher rate of infection was 76.1% in the age group (16 - 20) years while the lower infection rate was in the age group (26 - 30) years that was 22.8%. It has been observed significant differences in infection rates at the (P ? 0.05). The present study recorded difference in the rates of parasitic infections according to the presence of animals in houses, the high rates of infection where with those have animals in their houses 51.2% while the lowest rates of infection where with those not have animals in their houses 38.9%.It has been observed significant differences in infection rates at the (P ? 0.05). This study reveals a difference in parasitic infections rates, according to the level of education the head of household, and found that the highest rate of infection for those with the head of the family is non - educated or illiterate 48.3%, while the rate of infection was declined with head of the family who get primary education level 46.2%. It had recorded the highest infection rate in October 63.8%, while the lowest percentage was in February 27. 6%. It has been observed significant differences in infection rates at the (P ? 0.05). In the present study Polymerase chain reaction (PCR) is used to 96 positive results in direct smear methods to detection the main parasitic diarrhea agent. It had recorded total of infection rate of 43.4% (31.3%, 28.1% and 2.1%, respectively). Depending on PCR technique showed the highest rates of infection was in the male 36.7% while the lowest rates of infection in females 30.6%. It had recorded the highest rate of infection in the rural areas 45.3% in comparing rate of infection in the urban areas 25.9%. The present study recorded the highest rates of infection were in the (16 - 20years) age group 46.2%, while the decline in the rates of infection was in the (21 - 25years) age group 16.7%. Also it showed the highest rates of infection were in the presence of animals in the houses 36.1% while the lowest rate of infection where there were no animals 31.4%.It has been observed significant differences in infection rates at the (P ? 0.05). The study found that the highest rates of infection for those with the head of the family is non - educated or illiterate 38.9%, while the rate of infection declined with head of the family who got Academic education level 33.3%. Also it had recorded the highest rate of infection in November (2014) 42.1%, while the lowest rates percentage was in December (2014) 23.1%. It has been observed significant differences in infection rates at the (P ? 0.05). Through the current study it was concluded that the prevalence of the parasites that cause diarrhea in the Babylon province are very highly when detection microscopic examination and PCR method, comparison with previous studies and rural area highest rates of infection from urban area.

التوصيف المظهري والجزيئي لبعض الفطريات الخيطيه والخمائر المعزولة من اخماج العين واختبار حساسيتها الدوائيه تجاه بعض المضادات الفطرية == Morphological And Molecular Identification of Some Filamentous Fungi And Yeast Isolated From Eye Infections And Theirs Susceptibilities Toward Some Antifungals

Author name: يسر فاضل عبد الامير الاسدي
Supervisor name: زيدان خليف عمران المعموري
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Babylon - College Of Science For Girls - Department Of Biology
Language: Arabic
University location: Babylon
First pages:
Abstract: تمت هذه الدراسة في مختبر التقنيات الاحيائية التابع لكلية علوم البنات / جامعة بابل للفترة من اكتوبر 2012 الى اكتوبر 2013، حيث تم جمع 165 عينة سريرية من المرضى المراجعين لاستشارية العيون في مستشفى الحسين العام ومن بعض العيادات التخصصية لاطباء العيون في محاف | The study was performed in Biotechnical Laboratory in Science collage for women, Babylon University from Oct.2012 to Oct.2013. 165 clinical samples were collected from patients with eyes infections were admitted to AL - Hussein main hospital in Karbala province and private clinics of eye physician, after diagnosis of eye infection by specialize physician the clinical samples collected by using swab from Cornea, Conjunctiva, Lacrimal sac, eye allergic, contact lenses and from pull water eye after surgery, the samples included different sex & age groups.All clinical samples culturing on SDA and PDA, Yeast was identified base on their color reactions on Chromo agar Other biochemical test were performed.Antifungal sensitivity was performed against 8 antifungal (Nystatin, Econazol, Amphotericine B, Flucosytosine, Miconazole Metronidazole, Griseofulvin, Ketoconazol, Most fungi showed variable degrees in their susceptibilities to the antifungal agents. In this study we diagnostic (57) isolate from filamentous fungal species, Aspergillus spp. and Fusarium spp. showed highly frequent in ratio 20.1% and 9.6, In addition (37) yeast isolated, C.albicans and C.famata representative the highest frequent isolates.Molecular identification and genotyping, 8 isolates identified as C.albicans from 24 of selective Candida isolates were performed by specific pair primer (CABC) that give genotype with 515 base pair, genotype ITS1/ITS4 region show polymorphic PCR product help in diagnosis other (7) Candida species like C.famata, C.rugosa, C.glabrat, C.utili, C.saitoana, C.guilliermondii, C.inconpicua. The pair primer for amplified the transposable intron (CA - INT) was succeed in amplification of intron showed two genotypes : type A (450bp) and type B(850bp) and showed there was no C.dubliniensis isolates in our Candida isolate, This result was coincidence typing results between specific pair primer (CABC) and Microsatellite CAI for C.albicans.The amplified 18s region by using NS/EF3 and the result give genotype with molecular weight 1700bp.Also the study include using RFLP technique & the digested by using CfoI enzyme & the results for ITS PCR products of 24 isolates of Candida showed unique RFLP patterns.

تقيم معارف العناية الذاتية للمرضى المصابين بداء السكري النوع الثاني في مركز السكري في محافظة بابل == Assessment Of Self - Care Knowledge Among Type II Diabetes Mellitus Patients At Diabetic Center In Babylon Governorate

Author name: سالم كريم هجول الجبوري
Supervisor name: سلمى كاظم جهاد
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: اجريت دراسة وصفية (عرضيه مقطعيه) للفترة من 20/10/2015 ولغاية 25/6/2016 من اجل تقييم معارف الرعاية الذاتية لمرضى السكري النوع الثاني في مركز السكري في محافظة بابل. وقد اختيرت عينة هادفة من (120) مريضا باستخدام تقنية اخذ العينات غير الاحتمالية، التي تشمل ا | A descriptive design cross - sectional study has conducted throughout the period of October 20th 2015 to June 25th 2016 in order to assess the self - care knowledge of patients with type II diabetics mellitus at diabetic center in Babylon Governorate. A Purposive sample of (120) subjects were selected throughout the use of non - probability sampling approach, that include patients who were diagnosed with type II diabetes mellitus visiting diabetic center in Merjan medical city/ Babylon Governorate. A questionnaire was constructed to achieve the objectives of the present study, comprised of four domains including (50) items. Data was collected from patients attending for or treatment diabetic center in Hilla city through the use of interview technique, during the duty shift for the purpose of receiving health care, each patients' individually interviewed, helped by the researcher and taking a corresponded period of between ( 20 - 25) minutes. The questionnaire was clear and adequate for the measurement of the phenomenon except some adjustments are made on few items according to the experts' suggestions.Reliability of the questionnaire was determined through a pilot study and the validity was achieved through a panel of (16) experts. Data were analyzed through use of the Statistical Package of Social Sciences (SPSS, Version 20). Reliability was concerned with the consistency and dependability of the research instrument. Determination of internal consistency, reliability of the questionnaire is based on computation of Alpha Cronbach's Correlation Coefficient.The findings of the study indicated that Type II diabetes mellitus age group have old age. Sample less opportunity to read and write.Results of the diabetes mellitus patients demonstrated that sample acceptable level of socio - economic status. Regarding residency it was shown that most Diabetes mellitus type II patients existed in urban areas. study sample was found to score body mass index of overweight feature. Fortunately participants showed a good knowledge about the diabetes mellitus and its complications. In regard to knowledge of sample about self - care it was demonstrated that their achievements were fine. Self - care in diabetes has been defined as an evolutionary process of development of knowledge or awareness by learning to survive with the complex nature of the diabetes in a social context. The recent study summarized that some dietary behaviors need more attention from the patient to regulate and balance the condition, It is interesting to find out that sample study had reached good scores in regard to different issues of self - care except with some exercises and activities related to foot care. As well as current study concluded that Patients' age, levels of education, family history of diabetes and marital status affect their knowledge level as overall. The study recommended that. Patients with chronic diseases like the current one need to be educated in regard to some misconceptions such as treatment of the illness, Although majority of patients know the signs and symptoms of disease, health care facility can help in emphasizing the lack of information in some areas of the subject, Some self - care results show deficits concerning the diet, appropriate strategies such as give priorities to those very vital factors in controlling blood sugar when teaching patients can be used to correct the patients dietary habits. Educational materials or programs designed to assist Patients in practicing some activities to reduce burden of the condition.

العوامل المؤثرة على نوعية حياة المرضى المصابين بالجلطة الدماغية في مركز الفرات الاوسط للعلوم العصبية في مدينة النجف الاشرف == Factors Affecting Quality Of Life For Patients With Cerebrovascular Accident In Middle Euphrates Neuroscience Center In Al - Najaf Al - Ashraf City

Author name: احمد صالح رضا القاضي
Supervisor name: سحر ادهم علي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: خلفية الدراسة : تعتبر الجلطة الدماغية سبب رئيسي مؤثر يقلل مستوى نوعية حياة المرضى. قياس نوعية الحياة للاشخاص الذين يعانون من امراض مزمنة يعطي رؤية واضحة لتحديد اثره على مقدمي الرعاية الصحية عندما تكون النتيجة غير ممكنه. العوامل المؤثرة على نوعية الحياة لم | Background : Cerebrovascular accident is a main leading cause affectedly decrease the level of quality of life of the patients. The measurement of quality of life for population with chronic diseases gives a clear view to determine the impact on health care providers when prognosis is not possible. Factors affecting the quality of life may be vary between different age categorize such as CVA young and old CVA patients, different factors have affected the quality of life, related to the following aspects : motor impairment, physical dysfunction or dependency in activity of daily living, the presence of depression ,cognitive impairment, and speech disturbances.The main aim of the study : To identify the factors affecting the quality of life for patients suffering from cerebrovascular accident.Design of the study : Descriptive cross - sectional study design was selected to achieve the objectives of this study the factors affecting quality of life for patients diagnosed with cerebrovascular accident in Middle Euphrates Neuroscience center in Al - Najaf Al - Ashraf City, for the period between 27 October 2015 and 30 August 2016.Setting : A unique center was selected to conduct the study, the outpatient department in Middle Euphrates Neuroscience center in Al Najaf Al Ashraf City are used to collect the data.Sample of the study : Non probability ( purposive sample ) from patients with CVA (N=80) were selected to find out the factors affecting quality of life for patients with cerebral vascular accident. Data was collected by using an assessment questionnaire which was adopted and developed by the researcher to assess the factors affecting the quality of life for patients with cerebrovascular accident. It is divided of three parts : demographical data as the first part, clinical information is the second part, while the specific CVA quality of life questionnaire which extended to 48 items distributed upon 12 main domains as (energy and activity, family relationship, speaking, movement, mood, personality, self - care, social role, thinking, in addition upper extremities functions, vision, and work / productive ) is the third part. Validity of the questionnaire obtain by (19) experts, who work in different fields to obtain the reability of the questionnaire alpha cronbach was used as a statistical method.The majority of the study sample were between (59 - 69) of age. The highest percentage of the study sample were males and were married. On the other hand the highest percentage of the sample were illiterate , housewives and urban area residents. The scores of the quality of life of the majority of the study sample were high effected regarding the following domains : (energy and activity), plus movement, moderate effect presented related to the family relationship, speaking, mood, self - management, social role, thinking, function of the upper extremities, vision, and job / productive, while low effect score recorded for personality.Conclusion : The overall quality of life level of the CVA patient who participate in the study was moderate. The QOL for patient with CVA were significantly effected related to their age, occupation, and educational level. Chronic diseases, type of CVA and it's duration play as highly effecting factors related to the patient's quality of life.Recommendation : Establishing structured program realizing the challenges that might face the post CVA patients because this long journey need special planned care to improve patient's quality of life by special rehabilitation unit, the unit services may be extended for the patient's family.

الانماط الجينية والبروتين الرابط للرتينول 4 في مرضى الاعتلال الكلوي السكري النوع الثاني == Gene Polymorphism And Retinol Binding Protein 4 In Type 2 Diabetic Nephropathy Patients

Author name: احمد جبار عباس
Supervisor name: مها فاضل سميسم | علاء حسين ال حيدر
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
First pages:
Abstract: داء السكري هو مرض ايضي له تاثير على ايض الكاربوهيدرات والدهون والبروتينات. ومن مضاعفات السكري اعتلال الكلية السكري، الذي يعد السبب الرئيسي للفشل الكلوي المزمن. الليبوكالينات هي عائلة من البروتينات التي تنقل الجزيئات الكارهة للماء صغيرة مثل السروئيدات | Diabetes mellitus (DM) is a metabolic disease involving carbohydrate, lipid, and protein metabolism. Diabetic nephropathy (DN) is the significant complication of diabetes, which is at the present time the major cause of chronic renal failure. The lipocalins are a family of proteins which transport small hydrophobic molecules such as steroids, retinoids, and lipids. Retinol binding protein 4 (RBP 4) is a member of the lipocalin family of proteins and it has a role in insulin resistance. A meta - analysis demonstrated a significant association between the polymorphism of glucose transporter1 gene with DN. The presence of endothelial nitric oxide synthase (eNOS) polymorphisms may contribute to a decreased eNOS activity and a lowered nitric oxide level, and has been reported to be a potential factor in the development and pathogenesis of DN. The study aimed the evaluation of lipid profiles and determination of their association with retinol binding protein 4 expression in diabetic patients, evaluation of gene expression of eNOS G894T polymorphism in diabetic patients to predict its relationship with nephropathy in future, and evaluation of gene level of GluT1 polymorphism in diabetic patients to predict its relationship with nephropathy in future. This study was conducted in Hilla city, from December 2015 to April 2016. The samples were collected from Babylon Center for Diabetes and Endocrinology in Marjan Teaching Hospital in Babylon / Hilla city.This study was included 160 subjects, who were (35 - 58) years old and BMI between (25 - 29.9) which were divided into groups : Control group 80 apparently healthy persons included 40 male control group (MC) and 40 females control group (FC). Eighty patients with diabetes mellitus type 2 include (40 males group (M)) and (40 females group (F)). Blood samples (10 ml) were taken from fasting subjects. Two milliliters of blood were put in EDTA tube used for the determination of HbA1c and genetic study and 8 milliliters of blood were put in plane tube and centrifuged to separated serum to measure (glucose, insulin, retinol binding protein 4 and lipid profile ( and insulin resistance was measured by equations. The results show : 1. There is a significant increase in the mean of fasting glucose, HbA1c, fasting insulin level, insulin resistance in groups M and F as compared to the control groups, (p<0.01), but there was no significant increase between two patient groups (p ? 0.05)2. The significant increase (P <0.01) in total cholesterol (TC), TG, LDL - C and VLDL - C, while serum HDL - C concentration was found significantly decrease (P<0.01) in patient groups as compared with the control groups, and no significant increase or decrease in lipid profiles in comparison between patients groups (P ? 0.05).3. There is significant increase (P< 0.01) in RBP4 between patients and control groups, and not significant (P?0.05) difference between patient groups.4. Group M shows significant positive correlations (r = 0.514, p= 0.0012 and r = 0.536, p = 0.0034) of RBP4 concentration with serum fasting insulin concentration and HOMA - IR respectively, in group F, the results show a significantly positive correlation (r = 0.502, p = 0.001 and r = 0.474, p = 0.02) between RBP4 concentration with serum fasting insulin concentration and HOMA - IR respectively.5. A significant positive correlations was found (r = 0.486, p 0.001; r = 0.388, p 0.013; r =0.385, p=0.014) for serum RBP4 concentration with TC, TG and LDL - C concentrations respectively, and significantly negative with HDL - C (r = - 0.424, p =0.006) in group M, also positive correlation (r = 0.443, p =0.004; r = 0.467, p = 0.002; r = 0.435, p = 0.005) of RBP4 with TC, TG, LDL - C respectively and negatively with HDL - C (r = - 0.453, P = 0.003) in group F.6. A significant increase (< 0.01) in non HDL - C between patients and control groups and no significant (?0.05) difference between patient groups.7. According to the results of genotyping, XbaI polymorphism wasidentified as homologous genotype XbaI ( - / - ) were 12 (30%) in the group M, 14 (35%) in the group F, 3(7.5%) in the group MC and in the group FC 2(5%), while individuals have heterozygous genotype XbaI ( - /+) were 7 (17.5%) in the group M, 10 (25%) in the group F, 10 (25%) in group MC and in the group FC 8(20%).8. A significant association between the frequency of XbaI ( - / - ) variant in group M in compared with group MC an odd ratio = 5.14 and confidence interval at 95% level of (1.36 - 19.4), and in group F in compared with group FC an odds ratio = 13.12 and confidence interval at 95% of (2.64 - 65.07). 9. According to the results of genotyping, G894T polymorphism for eNOS gene was identified as homologous genotype TT were 7 (17.5%) in group M, 9 (22.5%) in group F, 8 (20%) in group MC and 10 (25%) in group FC, while individuals have heterozygous genotype G/T were 21 (52.5%) in group M, 20 (50%) in group F, 6 (15%) in group MC and 7 (17.5%) in group FC.10. A significant frequency of GT variant in group M is compared with group MC with odds ratio = 7.58 and confidence at 95% level of (2.43 - 23.62), and in patients of group F as compared with group FC with odds ratio = 7.27 and confidence interval at 95% of (2.4 - 22.02).In conclusion, the RBP4 is associated in causing insulin resistance and lipid abnormalities. The high levels of Non HDL - C in diabetic patient contribute in progression of diabetic nephropathy. The GLUT1 polymorphism in diabetic type 2 patients specially patients with XbaI ( - / - ) and eNOS gene polymorphism G/T allele have a role in progression to diabetic nephropathy.

قياس تركيز الحديد والفرتين والارثروبويتين وفيتامين ب وحامض الفولك والبيروكسايديز الدرقي في مصل مرضى تضخم الغدة الدرقية حديثي التشخيص في محافظة بابل == Serum Iron,Ferritin,Thyroid Peroxidase ,B12,Erythropoietin And Folate Concentrations In Patients With Newly Diagnosed Hyperthyroidism In Babylon Province

Author name: سالينا عبد العباس ناصر الشمري
Supervisor name: محمد عبيد المحمدي | علاء صادق العواد
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Nursing - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: فرط نشاط الغده الدرقيه : هو مرض يصيب الغده الدرقيه وذلك نظرا لارتفاع هرمون تي ثلاثه وتي اربعه وهما هرمونات الدرقيه ولوحظ ان هذا المرض يصيب النساء بنسبه اعلى من الرجال بسبب التاثير الهرموني للاناث مما يجعلها الاكثر عرضه لهذا المرض,من علامات هذا المرض هي زيا | Background : Hyperthyroidism is endocrine disease that occur due to high levels of thyroid gland hormones and this disease affected female more than male due to hormonal effect, the female is more susceptible to this disease.Sign of disease including increase in metabolic rate of patient,sweating,increase pulse,enlargement of thyroid gland(goiter) which by this sign can diagnosis the patient.Objective : Evaluate serum erythropoietin and its relation with increase metabolic rate as well as hemopoiesis,evaluate serum folic acid and vitamin B12 and its role in hyperthyroidism ,evaluate the correlation of thyroid peroxidase with thyroid hormones and evaluate serum iron and ferritin.Materials and methods : This study was done from October/2015 till July/2016.The total number of patients group in this study was seventy man and women.their age ranged 26 - 65 years,58 women and 12 men who was in patients group and 35 female and 5 male in control group.Patients data in this study was history of hypertension ,parity ,age and sex. The difficulty in my research was in collection of spa icemen from patients.Result : The result which obtained from this study found that high significant increase in ferritin,thyroid peroxidase,folate and erythropoietin (p?0.01) in patients as compare to control group.High significant decrease in iron and B12(p?0.01) in patients group compare to control group. in case of the age found the 30 - 39 years more effected age among patients group and the female more effected with disease than male.Conclusion : From this study we conclude that thehyperthyroidism in female more than male due to hormonal effect. Reduce in iron due to high metabolic rate lead to decrease absorption of iron and B12 which lead to anemia.increase thyroid peroxidase which play a role in T3 and T4 synthesis.

التزام مرضى احتشاء العضلة القلبية باتجاه العناية الذاتية في مستشفيات الحلة التعليمية == Adherence Of Patients With Myocardial Infarction Toward Self - Care Management In Hillah Teaching Hospitals

Author name: هادي جواد كاظم
Supervisor name: سحر ادهم علي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: يمكن وصف العناية الذاتية كبرنامج متعدد الابعاد للمرضى الذين يعانون من احتشاء عضلة القلب والتي تشمل الامور المتعلقة بحالتهم وتعزيز استراتيجيات الرعاية الذاتية مثل المراقبة الذاتية والتصرفات الذاتية تحسين معرقة المريض الايجابية واستهلاك نظام غذائي صحي، الاد | Self - management can be described as multidimensional program for patients with myocardial infarctions which include improvement of the patient s knowledge related to their condition and enhance self - care management strategies such as self - monitoring, positive self - behaviors, and consumption of healthy diet, medication and following the health team advices. This strategies decrease hospital readmissions and improve quality of life. The aim of the study is assessing the adherence of self - care management among patients with myocardial infarction. To identify the demographical characteristics of the study sample such as (age, gender, marital status, educational status, and addressing, economic status). To find out the relationship between patient adherence of self - management and demographic data (age, gender, marital status, educational status, addressing, economic status) and clinical characteristics.Across - sectional descriptive design is used to carry out the study of assessing the adherence of the self - care management for patients with myocardial infarction in Hillah teaching hospitals from 27 - 10 - 2015 to 20 - 8 - 2016.Coronary care units and medical wards in two teaching hospitals in Hillah city were selected to conduct the study. A Non - Probability (purposive sample) of (100) patients, (64) male, (36) female have been selected after obtaining their agreements to participate in this study, these patients have been admitted to the medical wards or coronary care units. Data were collected by using structured questionnaire which prepared to assess the adherence of self - care management for patients with myocardial infarctions after a comprehensive review of related literatures. The questionnaire contains three parts, the first part consist data related to the socio - demographical characteristics of the sample, the second part includes clinical information such as (duration of disease, health history, body mass index...etc.), while the third part extends to collect the data related to the patients adherence toward self - care management which distributed up on (4) domains consisting nutritional domain (27) items, healthy behaviors (7) items, medications (8) items, medical follow up which has been extended to (9) items. Data have been collected by using face to face interview.To test the content validity the questionnaire distributed among (25) experts who have not less than 10 years of experience , the reliability was calculated through applying coefficient of correlation and inter rater reliability, descriptive and inferential statistics methods used to determine the results of the current study.The majority of the study sample have been (64%) male between (60 years and more) age group, (64) married, (22%) have been illiterate, (66%)of the sample urban area residency. housewives Most of them (25%) suffer from hypertension as a chronic disease. Overall adherences of the self - care management of the participant poor related to diet, healthy behaviors, follow up while they recorded moderate adherence related to consumption of medication.The majority of the patients who participate in the study were with moderate adherence relation to self - care management.Establishment of well - equipped rehabilitation center specialized to improve patient's knowledge and practices related to their conditions, and help them to live with their condition, the services of this center may be extended to serve patient's family.

اعباء رعاية الابوين للاطفال المصابين بالتخلف العقلي في محافظات الفرات الاوسط == Parents Burden Of Care For Children With Mental Retardation In The Middle Euphrates Governorates

Author name: علوان حسن رزن الجبوري
Supervisor name: سجاء هاشم محمد الربيعي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: يمكن ان تسهم مسؤولية رعاية الاطفال المتخلفين عقليا في عبا على الوالدين, الدراسة تشير الى ان اباء الاطفال الذين يعانون من التخلف العقلي لديهم مستوى عال من العبء.دراسة تحليلية وصفيه لتقييم عبء رعاية الاباء للاطفال ذوي التخلف العقلي في محافظات الفرات الاوسط | The responsibility of caring for mentally retarded child can contribute to parents burden. The study shows that parents of children with mental retardation experience high level of burden.The present study aims to assess the level of burden of care among parents who have children with mental retardation.and to identify the relationship between parents burden of care for children with mental retardation and their sociodemographic data such as age, gender etc. A descriptive - analytical study design is conducted to assess the Parents Burden of Care For Children With Mental Retardation in The Middle Euphrates Governorates and its relation to children and parent's sociodemographic characteristics and clinical characteristic of mental retardation child.1st October 2015 to 1st October 2016. A purposive (non - probability) sample of (90) parents (mother or father) of child with mental retardation are selected attending mental disability care institute. Data was collected through the use of The questionnaire which are constructed by the researcher for the purpose of present study. In order to test the validity of the questionnaires, the instrument is presented to panel of experts in different fields to make it more valid. A preliminary copy of the questionnaire is displayed to (15) experts. Data have been analyzed by using descriptive and inferential statistics.The results of the study indicated that parents exhibit high level of burden due to the handicap caring among their children. There is a significant relationship between the levels of parents' burdens and their child handicap degree and the monthly income. As for the psychological status there are significant differences between the psychological burden and the parents level of education and residency. But there is non - significant statistical points between the parents burden and other parents demographical data.The study concludes that there is a high level of parents burden due to the presence of mental retardation child in the family and that effect on the family. The degree of mental retardation is very important indication about the level of the burden.It is recommended that future study researches should be directed towards exploring psychological intervention factors which help to minimize the burden of care on parents of children with mental retardation.

المشكلات النفسية الاجتماعية لدى كبار السن في دور رعاية المسنين في محافظات الفرات الاوسط == Psychosocial Problems Among Elderly People At Geriatric Homes In The Middle Euphrates Governorates

Author name: وسيم عبد الامير شاكر البصام
Supervisor name: سجاء هاشم محمد الربيعي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: اجريت دراسة وصفية تحليلية خلال المدة من الاول من تشرين الاول من عام 2015 الى تشرين الاول 2016 من اجل تقييم المشكلات النفسية الاجتماعية لدى كبار السن في دور رعاية المسنين في محافظات الفرات الاوسط.عينة غرضية (غير احتمالية) تتالف من (82) شخص مسن قد تم اختيا | Analytical descriptive study was conducted during the period of 1 October 2015 to October 2016 in order to assess the psychosocial problems among elderly people at geriatric homes in the Middle Euphrates Governorates. A purposive sample (non - probability) for (82) elderly people was selected from geriatric homes in the Middle Euphrates Governorates, information was gathered through the use of a questionnaire for assessing psychosocial problems, study tool consist of three parts which contain (53) items. Part one : The first part consists personal information and include age (year) , age at admission, residence years in geriatric homes, gender, previous professions, educational level, social status, economic status, financial support by family, the financial resources.Part two : The second part consists psychological problems (anxiety, depression and suicide ideation) which contains (35) item. Part three : The third part consists social problems (social isolation) which contains (12) item. Reliability is determined by using alpha correlation (Cronbach's Alpha) and the validity is achieved through a panel of (21) expert. Data analyzed through using the Statistical Package of Social Sciences SPSS (Version 20) performed through the use of descriptive statistical data analysis approach, of frequencies, percentages and inferential statistical approach which is presented as an analysis of variable chi - square.The findings of the study indicated that most of elderly people were suffering from psychosocial problems in moderate levels. The majority of the sample of elderly were in the age group (75 - 84) years and most of the elderly who spent in geriatric homes (1 - 4) years, males, divorced and uneducated. Results of the study showed the existence of a high significant relationship among the elderly who were uneducated and divorced which suffered from psychosocial problems.The study concluded that the psychosocial problems for elderly were in the age group (75 - 84) years and in moderate levels.The study recommended to improve care for elderly people and provide psycho educational programs for them to increase their knowledge toward the psychological problems and provide psychological support to improve their psychological status

الاهمية الكيميائية الحياتية لنبتة العليق الاحمر كماسك طبيعي في حالة زيادة الحديد == Biochemical Importance Of Rubus Idaeus As A Natural Chelator In Iron Overload Status

Author name: حازم علي حسين
Supervisor name: مفيد جليل عوض | مؤيد عمران الغزالي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
First pages:
Abstract: لمنع تراكم الحديد في الجسم، وخاصة لمرضى داء ترسب الاصبغة الدموية،او الذين يحتاجون الى نقل دم بصورة مستمرة، لابد من عمل مركب معه يعمل على استخراجه.نبتة العليق الاحمر تحتوي على كمية عالية من حامض التانيك وهو مركب متعدد الفينولات للنبات يعمل على مسك او حجز | To prevent iron overload, especially for those patient with hemochromatosis or those having chronic blood transfusion, iron must be chelated and excreted, Rubus Idaeus containing high quantity of tannic acid which is a plant polyphenol, chelate iron and excreted from the body. The present study aimed to evaluate the activity and efficiency of tannic acid in Rubus Idaeus fruit to reduce or eliminate iron overload through enhance direct impact on some of the body,s vital biological activities by using fresh crude plant as herbal medicine. This study is Cohort study designed. It is monitoring, detection, and investigation study that included some of groups that completely under control(control), and other group which induced iron overload , the iron overload group is subdivided to 5 groups, one of this sub gorups is treated with desferal(Deferroxamine),and the rest 3groups giving 3 different doses of pure fruit of Rubus Idaeus, while the least group is not treated with fruit or with desferalThe study included (60) Albino male mice divided into six groups with count (10) mice for each. First group was labeled as control (A). The other five groups received intra peritoneal injections of iron - dextran at a dose of 100 mg/kg/day B.w. (one dose every two days for 3 weeks) are the iron overload groups ,which are classified as a following : B1 group ,in which the iron overload induced mice treated with 100 mg/kg/day of Rubus Idaeus fruit, B2 group, in which the iron overload induced mice treated with 200 mg/kg/day of Rubus Idaeus fruit,B3 group ,in which the iron overload induced mice treated with 300 mg/kg/day of Rubus Idaeus fruit, group(C),in which the iron overload induced mice received 493mg/kg/day desferal by subcutaneous injection for 21 days beginning on the day following the first iron dextran injection, group (D) ,in which the iron overload induced mice neither received desferal nor Rubus Idaeus fruit. The experimental work was carried out in the animal house and in the Clinical Biochemistry Department / College of Medicine / Babylon University during the period from (October 2015 to August 2016). High - Performance Liquid Chromatography (HPLC) technique has been used for measurement of tannic acid in fruit in mice's serum. Enzyme Linked Immunosorbent Assay (ELISA) has been used for the measurement of serum ferritin, UV - Vis Spectrophotometer has been used for determination of GSH - Px and GST concentrations, while Graphite Furnace Atomic Absorption Spectrophotometer technique (GFAAS) has been used for concentration measurement of trace elements. There were hematological study have been carried out using special required equipment, protein analysis by electrophoresis has been used for mice's serum to detect protein changes for all groups. Results showed that Rubus Idaeus fruit contain good acceptable concentration of tannic acid up to (853 µg/gm),amount of trace element in the fruit plant was 14.12 ppm for Zinc,33.62 ppm for Iron,3.5ppm for copper(Cu),311.06 ppb for selenium(Se),40.13 ppb for chromium(Cr). Results also showed highly significant increase of Serum ferritin, hemoglobin levels, GST activity, Iron and Copper concentration, and decreased in chromium in iron overload group (D) (P<0.001) when compared with control group (A), while there were highly significant decrease of these parameters and increased chromium in group (B2) and group (C) as compared with control groups. Results also showed highly significant decrease of GSH - Px activity, Zinc and Selenium concentration, and increased in hemoglobin levels in group (D) (P<0.001) when compared with the control group, while there were highly significant increase of these parameters and decreased in hemoglobin level in group (B2) and (C)as compared with control and (A) groups. The study have been found positive effect of Rubus Idaeus on abnormal parameters and support the biological system which was clear in group (B2) and (C) which demonstrated that there were significant inhibitions to decrease or increase of these parameters than those in normal. The present study found that fruit of Rubus Idaeus in dose(200 mg/kg/day)in mouse(16.2mg/kg/day in human) gives more reasonable results than other 2 doses (100mg/kg,300mg/kg) in treatment of iron overload ,100mg/kg had no effect in reducing iron overload,300mg/kg chelate iron so more that causing anemia, while this study found a negative correlation between Ferritin and GSH - Px activity, serum chromium and Selenium concentration. Results also showed that there is a changes in protein bands in samples, new bands occurs in samples of induced iron overload group (D) due to increased production of iron regulating proteins. While these bands are absent in groups (A),(B2)

تقييم مضاعفات ما بعد العملية للمرضى الخاضعين لجراحة العظام في مستشفى الحلة التعليمي == Assessment Of Postoperative Complications For Patients Undergoing Orthopedics Surgery At Al - Hilla Teaching Hospital

Author name: احمد رامز هلال
Supervisor name: فخرية جبر محيبس الزبيدي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: الخلفية : مضاعفات ما بعد العملية تتعلق بنقص في العناية ما بعد العملية تقود الى عدة مضاعفات ربما تتطور في عدة مرضى نتيجة تاثير التخدير العام ومخاطر اخرى مثل طول فترة الرقود في المستشفى، عدم الحركة بعد العملية، التدخين, 000 الخ. كل عوامل الخطورة ربما تؤدي ال | Background : Postoperative complication is a source of concern as insufficient postoperative care leads to many complications which may develop in many patients due to the effect of general anesthesia and other risk factors such as prolonged hospitalization, immobilization after surgery, smoking, …etc. All risk factors may lead to postoperative complications, thus they impose additional economic burdens on the society and the patient.Objectives : The purpose of this study was to assess the postoperative complications in patients undergoing orthopedic surgery and to find out relationship between socio - demographic characteristics, medical information and postoperative complications such as (age, gender, smoking, medication, …etc.). Methodology : The study was carried out from 25 January 2016 to the 30 November 2016, the sample was Non probability sample which consists of (100) adults patients (male and female) who were undergoing orthopedic surgery with lower and upper extremities' under general anesthesia, without chronic disease and age of patients 18 years older at Al - Hilla Teaching Hospital.To achieve the objectives of the study, the researcher established the questionnaire format which was composed of three - part, part one : socio - demographic characteristic (5) items, while part two : consisted of medical information of patients which comprised of (7) items and part three : consisted of (34) items of postoperative complications may happen postoperatively.Results : The results of this study indicated that a high percent of postoperative complications occurred in ages between (18 - 27)years. The study also revealed the highest percentage (38%) of patients were smokers who had high complications. In addition the current study founded that smoking appears to decrease postoperative vomiting rate in smoker (16.2 %) less than non - smoker (36.7 %). The rate of vomiting in female (60%) is more than male (22.3 %). in addition the rate of infection in Smoker (11.6 %) more than non - smoker (1.7%). Total score of complications indicated the significant relationship between socio - demographic characteristics, medical information and postoperative complications like (age, smoking, occupation, education level, and medications used) as it is proved by chi - square tests. Recommendations : According to the current study, the researcher recommends to Increase knowledge of nursing staff regarding postoperative complications by continuing education , so the nurses can identify and prevent postoperative complication.Increase knowledge of patients regarding postoperative complications through provide them by advices or list in which is containing information or pictures that occur in patients with orthopedics surgery such as deep vein thrombosis, wound infection, and how to prevent them.

تقييم الحالة التغذوية للاطفال دون سن الخامسة من العمر في مدينة الحلة == Assessment Of Nutritional Status Among Children Less Than 5 Years Old In Al - Hilla City

Author name: اسماعيل حسن جواد الموسوي
Supervisor name: قحطان هادي حسين الجبوري | حسن علوان بيعي
General topic: Medicine
Specific topic: Nursing
Degree: Master
University: University of Babylon - Faculty Of Nursing
Language: English
University location: Babylon
First pages:
Abstract: اجريت دراسة مقطعية وصفية خلال الفترة من 1 تشرين الاول 2015 الى 27 اب 2016 لاجل تقييم الحالة التغذوية للاطفال دون الخامسة من العمر في مدينة الحله من خلال قياس (الوزن للطول والعمر) من خلال المقاييس الجسمانية, وكذلك لايجاد العلاقة ما بين الخصائص الديموغرافية

دراسة انزيم التيلومريز وجين الكلوتاثيون بيروكسيديز الاول في مرضى السكري النوع الاول == Telomerase Enzyme And Glutathione Peroxidase1 Gene In Type 1 Diabetes Mellitus Patients

Author name: دعاء مهدي هادي الحكاك
Supervisor name: مها فاضل سميسم | علي حسين البياتي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
First pages:
Abstract: Type 1 diabetes is characterized by an immune - mediated depletion of ? - cells that results in lifelong dependence on exogenous insulin. Accumulating evidence suggests that oxidative cell injury caused by free radicals contributes to the development of type 1 diabetes mellitus (T1DM) complications and decreased efficiency of antioxidant defenses (both enzymatic and nonenzymatic) seems to correlate with the severity of pathological tissue changes in T1DM, So many studies have tended to analyze the genetic material that can be related to the occurrence of the disease. Different combinations of genetic defects of antioxidant enzyme are expected. This could readily provide an explanation of the heterogeneity of T1DM.The aim of this study is to assess the differences of GPX1 genotype in T1DM as compare with controls. And the effect of C/T at nucleotide 1050450codon 198 of GPX1 gene polymorphisms on serum glutathione peroxidase (GPX) and telomerase enzyme (TE) concentration in T1DM patients and controls. This study included (108) persons, their ages between (28 - 42 years) and body mass index in normal and overweight (68) of them were uncontrolled diabetes type 1 (HbA1c ? 6.5 %), (34) of them were male patients (M group), the other (34) were female patients (F group), and the other (40) apparently healthy as control group (20) male (MC group) with (20) female (FC group). The sera sample of all groups were used to measure the concentration of fasting blood glucose by using colorimetric methods and ELISA method for determination (TE), total antioxidant capacity (TAO - C), glutathione (GSH), and glutathione peroxidase1 enzyme, while whole blood samples from study subjects were used to measure HbA1c and extract DNA for the study of polymorphisms in GPx1 gene.The results of the present study showed1 - A significant increase in glucose, HbA1c in both male and female patients compared with controls group. (P < 0.0001) and (p< 0.05) respectively.2 - The study showed significant decrease (p? 0.001) in telomerase enzyme in both M and F as compare with MC and FC groups,3 - There was no significant association between M and F (P=0.106) and between MC and FC (P=0.327) regarding to gender.4 - The result showed that telomerase concentration significantly decrease with age (p - value<0.001) which indicated by negative correlation between telomerase concentration and age.5 - The result showed significant negative correlation between telomerase enzyme concentration and BMI in patients and controls.6 - The result showed significant decrease in TAO - C in patient as compared with control groups (P=<0.001)7 - GPX1 concentration significantly decreased in patient as compared with control groups (p - value <0.001 )8 - Significant increase in Glutathione concentration in patients as compared with controls (p - value <0.05)9 - Data on the genetic patterns were analyzed using polymerase chain reaction technique - RFLP revealed, were defined as : the codon 198 of Gpx1 genotyping have the homozygous wild - type (CC) Pro/Pro, the homozygous mutant (TT) Leu/Leu, and heterozygous (CT) Pro/Leu allele. There was significant difference between genotype distribution and frequency of alleles between the two groups of patients (M and F) versus (MC and FC) (odds ratio = 33, into confidence level of 95% : 1.65 to 656.26) and P value ( 0.0219) for M vs. MC, and (OR = 12.60, into the proportion of 95% confidence level : 1.93 to 82.08) and P value (0.0081) for F vs. FC. Also, the frequency of TT genotypes in GPX1gene in male and female were47% and 61.7% compare to control male(0%) and female(10%).10 - The allele frequency showed significant differences in all sample (p=0.0013), in male (0.14) and in female (0.0020).11 - There was significant difference in telomerase concentration (p= 0.001) and in glutathione peroxidase concentration (p< 0.0005) in patients group depending on different of the GPX1 genotyping.12 - Depending on GPX1 genotype there were significant differences in BMI in normal weight and in overweight between patient and control (p=0.0375) and (p<0.0018), respectively.In Conclusion, the TT genotype of The GPX1gene variants Pro 198 Leu may be risk factor to T1DM patients. TIDM significantly associated with glutathione peroxidase and telomerase level in patients with TT genotypes of GPX1 gene compared to those with CT and CC genotypes, and this may be considered as a risk factor for senescence and mortality in T1DM. patients, also there is significant negative correlation between telomerase enzyme and BMI in patients and controls.

التغيرات الحاصلة في نسب الانسولين والاحماض الصفراء والكوليسترول 7 الفا هيدروكسليز وفوق الاكسيد الفائق في مصل مرضى حصى المراره == Serum Insulin, Bile Acid, Cholesterol 7 - Alpha Hydroxylase And Superoxide Dismutase Changes In Patients With Gallstone

Author name: سماح سجاد كاظم الشمري
Supervisor name: محمد عبيد المحمدي | علي خير الله الشاعلي
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: يعتبر مرض حصى المرارةمن اكثر امراض الجهاز الهضمي شيوعا، ووتشكل عبئا كبيرا على انظمة الرعاية الصحية. معظم الناس (حوالي 80 ?) مع حصى المرارة ليس لديهم اعراض. وتشمل مضاعفات حصى المرارة التهاب المرارة والبنكرياس.الهدف من الدراسة : 1 - للتحقيق في العلاقة بين | A gallstone is a stone formed within the gallbladder out of bile components. Cholelithiasis or Gallstone disease (GD) is one of the most prevalent gastrointestinal diseases, with a substantial burden to health care systems. Most people (about 80%) with gallstones are asymptomatic. Complications of gallstones include inflammation of the gallbladder, pancreas. Objective 1. To investigate the association between serum level bile acid and cholesterol 7 - alpha hydroxylase and the development of gallstone disease. 2. To Evaluate the change serum level insulin changes and its effect on gallstone formation. 3. The determination of serum superoxide dismutase changes in patients with gallstone. Material and Methods In this study, one hundred sixty (160) patients with gallstone aged (20 - 70) years and one hundred sixty (160) age and sex match subject (control group) had involved in this study. The patients were intended to undergo surgical removal of gallbladder in Al - Hilla general teaching hospital during the period from November (2015) to May (2016). Blood samples were obtained from all patients prior to surgical treatment. Serum was obtained by usual methods and analyzed for insulin, insulin resistance Cholesterol 7 - alpha hydroxylase, Superoxide Dismutase, Bile acid (Deoxycholic acid and Chenodeoxycholic acid) and lipid profile {total cholesterol, triglyceride, high density lipoprotein - cholesterol and low density lipoprotein cholesterol}. And qualitative analysis of gallstone (Cholesterol stone, mixed stone, pigment stone), then these parameters compared with those of control groups. In addition, a demographic comparison regarding to the age, sex and body mass index between the patients and control groups was carried. Results The results of this study show higher significant percentage of gall stone in female (81%) than male (19%) in the patient group. There was an insignificant difference between male and female patients, according to age group (P<0.05). There is a higher significant (p>0.05) family history in the patients with gallstone compared with control subjects. Additionally, There is highly significant percent of female using oral contraception in pateints than control group (p > 0.01). Similarly, smoking, parity,Type 2 diabetes mellitus and Body Mass Index are found to be significantly higher inpatient group (p>0.01). This study demonstrate that insulin and insulin resistance are significant higher in sera of patients with gallstone as compared with the control group. (p>0.01) Regarding the cholesterol 7 - alpha hydroxylase, superoxide dismutase and Bile acids are found to be significantlylower in sera of patients with gallstone as compared with the control group, p< 0.01). It has also found that there are a higher significant difference in gallstone patients than control regarding total cholesterol, triglyceride, high density lipoprotein - cholesterol and low density lipoprotein cholesterol levels were significantly higher than that of control group (p>0.01). Meanwhile, all these parameters in the study are variated according to gender. However The qualitative analysis of stone that show cholesterol stone is more common than other types.Conclusions In this study the most studied parameters including of Insulin and insulin resistance, cholesterol 7 - alpha hydroxylase, superoxide dismutase and Bile acids, cholesterol, triglyceride, high density lipoprotein - cholesterol and low density lipoprotein cholesterol are significantly different in gallstone patients compared to control subjects and such differences are demographically affected depending on the age, sex, BMI, parity, contraceptive and smoking.

Effect Of SNP rs198389 of BNP Gene On The Natriuretic Peptides Levels In Cardiovascular Patients

Author name: Alaa Salman Idan Al - Ibrahimi
Supervisor name: Monem M. Al | shok | Moaed E. Al | Gazally
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
First pages:
Abstract: Acute coronary syndrome (ACS) refers to any group of acute signs and symptoms attributed to sever obstruction of the coronary arteries. Acute coronary syndrome usually occurs because of one of three problems : ST elevation myocardial infarction, non - ST elevation myocardial infarction, and unstable angina. These types are named according to the results of the electrocardiogram (ECG). In addition to clinical and electrocardiogram (ECG) findings, several biochemical markers are considered in patients with chest pain to diagnose myocardial ischemia. Heart failure (HF) according to American College of Cardiology (ACC) and American Heart Association (AHA) is a complex clinical syndrome that can be caused by any structural or functional disorder in the heart that fails the capability of the ventricle to fill with or to eject blood. The diagnosis is often performed clinically on the basis of the presence of dyspnea, fatigue, signs of fluid overload, such as pulmonary crepitation, peripheral edema, and distension of jugular vein.B - type natriuretic peptide (BNP) and N - terminal pro - BNP (NT - proBNP) are secreted by human atrial and ventricular myocardium. The rise in BNP and NT - proBNP levels after ACS and HF correlates with infarct size and regional wall motion abnormalities. Echocardiography remains the most frequently used and usually the initial imaging test to evaluate all cardiovascular diseases related to a structural, functional, or hemodynamic abnormality of the heart or great vessels. This study is designed to investigate and assess the serum concentrations of BNP and NT - proBNP in patients with HF and ACS; In addition, almost equal number of males and females are chosen to control the effect of age and gender on the levels of BNP and NT - proBNP. Also, the study aims to evaluate the effect of BNP gene T - 381C (rs198389) polymorphism on the plasma human levels of BNP and NT - proBNP and compare the results that will obtain with healthy control.The present study was involved (70) patients, (35) of them with ACS and the other (35) with HF. The study also includes (22) subjects were taken as control group. The sera obtained from the blood of patients and control subjects were used to measure the concentrations of BNP and NT - proBNP, while whole blood samples from study subjects are used to extract DNA for the study of polymorphism in BNP gene.The results indicate both the ACS and HF groups have significantly higher levels of BNP and NT - proBNP when compared to control group, also HF patients have higher levels of both parameters than ACS patients, in all comparison (p

المستويات والانماط الجينية المتعددة للانترلوكين - 6 في مرضى التهاب المفاصل الرثوي في محافظة بابل/العراق == Interleukin - 6 Gene Polymorphisms And Levels In Rheumatoid Arthritis Patients In Babylon Province / Iraq

Author name: سارة حسين عيدان
Supervisor name: عبد السميع حسن الطائي | صباح جاسم الربيعي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Biochemistry
Language: English
University location: Babylon
First pages:
Abstract: التهاب المفاصل الرثوي هو احد الامراض المناعية الذاتية الالتهابية المزمنة الذي يتميز بالتهاب المفاصل المتماثل المستمر الذي يصيب عادة مفاصل اليدين والقدمين.تهدف هذه الدراسة الى التقصي عن العلاقة المحتملة بين مستويات الانترلوكين - 6 والانماط الجينية المتعددة | Rheumatoid arthritis (RA) is a chronic systemic inflammatory, autoimmune disease characterized by persistent symmetric polyarthritis that commonly affects joints of hands and feet.This study aims to investigate the possible association between interleukin - 6 (IL - 6) levels and ( - 174 G/C) IL - 6 promoter polymorphism in RA in Babylon Province. 60 RA patients and 60 healthy controls matched in age and sex were included in this case control study. Measurement of serum IL - 6 and serum anti cyclic citrullinated peptide (ACCP) antibodies were assayed using commercially available ELISA kit. Measurement of blood rheumatoid factor (RF) was assayed by use slide agglutination test for the qualitative and semiquantitative. Whereas, blood C - reactive protein (CRP) was determined using latex - enhanced nephelometry. Disease severity score of RA patients was determined by use DAS - 28. DNA was isolated from white blood cells (WBCs) and ( - 174 G/C) IL - 6 gene promoter polymorphism was determined by polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP) technique. the study was found significantly high levels of serum IL - 6 and ACCPA in untreated and in treated RA patients when compared to healthy controls. RF of patients with RA in the present study was found to be positive in 83.32% and negative in 16.67 % in overall RA patients, whereas was negative in 99% of healthy control. CRF of patients with RA in the present study was found to be positive in 78.4% and negative in 21.6 % in overall patients with RA, and was positive in 20% of healthy control. Correlation between DAS - 28 and IL - 6 levels in untreated and in treated RA patients were significant positive correlation. Correlation between IL - 6 and ACCPA in untreated and in treated RA patients were significant positive correlation. Correlation between DAS - 28 and ACCPA in untreated and in treated RA patients were significant positive correlation. The Genotype of ( - 174 G/C) IL - 6 gene promoter polymorphism and its percentage in patient found to be 80% GG,18.3% GC and 1.6% CC, whereas in healthy control 98.3% GG, 1.6% GC and 0% CC. Relation between both of IL - 6 levels and DAS - 28 with genotyping of ( - 174 G/C) IL - 6 gene promoter polymorphism in untreated and treated RA patients were found to be positive significant relation. The high levels of IL - 6, and its positive relation with DAS - 28 may suggest a possible role of IL - 6 in the pathogenesis of RA. Moreover, these biomarkers can be used as markers of disease activity in the diagnosis and treatment of RA. ( - 174G/C) IL - 6 polymorphisms are also associated with the RA risk, and the C allele of IL - 6 promoter polymorphisms has dramatically increased the susceptibility of RA in Babylon population. This finding suggests that the ( - 174 G/C) IL - 6 gene promoter polymorphism may also be used as another genetic marker for the onset and development of RA in Babylon population.

الكشف عن طفر الجين عامل النمو البشراوي الثاني (HER2) في سرطان الخلايا الانتقالية باستعمال طريقة اللونية في موقع التهجين == The Detection Of HER2 Gene Mutation In Transitional Cell Carcinoma Using CISH Method

Author name: عمار عيسى مهدي
Supervisor name: احمد تركي عبيد | هادي محمد علي الموسوي
General topic: Medicine
Specific topic: Clinical Diseases
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Diseases
Language: English
University location: Babylon
First pages:
Abstract: يعتبر سرطان المثانة واحدا من اكثر انواع الاورام السرطانية التي تصيب الجهاز البولي حيث انه يعتبر ثاني ورابع الاكثر شيوعا للذكور في العراق والولايات المتحدة على التوالي وايضا يعتبر العاشر والحادي الاكثر شيوعا للاناث في العراق والولايات المتحدة على التوالي. | The urinary bladder cancers are among the common types of cancer of the urinary tract. It is regarded as the second and the fourth most common cancer types for males in Iraq and in the United State of America (USA) respectively and as tenth and eleventh most common cancer types for females in both Iraq and USA respectively. Urothelial Carcinoma is epithelial tumor of urinary bladder (95% of urinary bladder tumor) and it shows wide range of tumor that begins from papilloma and carcinoma in situ to invasive cancer. The patients with urinary bladder cancer that are presented in non - muscle invasive type or muscle invasive type show different rates of HER2 gene amplification (specially for invasive muscle type). Epidermal growth factor receptor (EGFR, also called HER or type I ErbB tyrosine kinase receptors) family is composed of four receptor member which include HER1 (ErbB1 or EGFR), HER2/neu (ErbB2 or C - ErbB2) , ErbB3 ( HER3), and ErbB4 (HER4). HER2/neu protein is glycoprotein produced by HER2 gene which lies in 17q21 (long arm of chromosome 17). This glycoprotein is normally found in lung, breast, urinary bladder, prostate and stomach, and its cytoplasmic portion has enzymatic function (tyrosine kinase activity). This receptor is responsible for the cell survival, normal cell growing and inhibition of apoptosis. HER2/neu protein overexpression is measured by IHC when the result of IHC is equivocal to HER2/neu protein (+ 2). Then, HER2 gene amplification is measured by using an accurate and more practical CISH method. The aims of this study are tried to detect the percentage of HER2 gene amplification in the urothelial carcinoma and distribution of HER2 gene amplification with clinical criteria (age , sex, grade, stage, and location of tumor) of the patients that had urothelial carcinoma of bladder.This study contained retrospective (30 cases) and prospective (20 cases) and was conducted in Al - Hilla Surgical Teaching Hospital and the period of the study extended from October /2015 to August/2016. Clinical data of patients include age, gender , clinical features, and type of bladder biopsy ( endoscopic resection or cystectomy) are taken with paraffin blocks.The practical part of the study was done in the laboratory of the department of pathology / College of Medicine/Babylon university. It included 50 patients with bladder cancer (35 males and 15 females ) with two groups (10 cases is positive and 10 cases is negative). It included 50 patients who were diagnosed with transitional cell carcinoma of urinary bladder with mean age ± SD ( 66.04 ± 10.148 ) years old ranging between 42 - 83 years old. Also, Positive and negative control slides applied with each run CISH method (+ve control of breast tissue that treated by immnohistochemical procedure ( score +3) and the same tissue were applied by removing HER2/neu probe).Fifty patients who were diagnosed with transitional cell carcinoma of bladder and their biopsies of bladder ( taken from endoscopic biopsy or cystectomy). Then, paraffin - embedding procedure was prepared on tissues biopsy and then, hematoxylin and eosin staining (H. and E. staining) procedure was done. Finally, manual CISH was done on both cases(positive and negative cases) and bladder cancer blocks. This CISH method detected her2 amplification according to ASCO/CAP/2013 scoring system. The results showed that age group 61 - 70 years was the highest positive HER2 amplification (64.7%) among other age groups 51 - 60 years (11.8%) and 71 - 80 years old (23.5%), there was a significant association between these age groups with HER2 amplification , p? 0.05. Sex group displayed that 52.9% of male and 47.1% of female who had positive HER2 amplification and there was no significant difference between sex groups with HER2 amplification, p > 0.05. Other results showed that positive HER2 amplification was highest in grade 3 (88.2%) of transitional cell carcinoma when compared to grade 2 (11.8) and grade 1 (0%) and there was significant association between cancer grading system with HER2 amplification, p? 0.05. MIBC and NMIBC of staging cancer system showed 82.4% and 17.6% positive HER2 amplification respectively with a significant difference between them ( p value ? 0.05). Also, other result of the histological types of transitional cell carcinoma revealed 88.2% and 11.8% (positive HER2 amplification) of papillary and flat shape respectively with a significant correlation between them ( p value ? 0.05). The HER2 gene amplification was correlated with grade and stage of bladder carcinoma which showed high amplification in high grade and stage. The HER2 gene amplification was not correlated with the sex of the patients, but more cases of HER2 gene amplification were recorded in males; while there was a correlation with age group specifically (61 - 70 years old). The HER2 gene amplification was associated with aggressive behavior of blabber carcinoma. The CISH method application in HER2 gene amplification was flexible and applical.

التوصيف الجزيئي لعوامل الضراوة المرتبطة باصابات بكتريا المكورات العنقودية السالبة للتخثير والمعزولة من المصابين بالتهاب المجاري البولية في محافظة بابل == Molecular Characterization Of Some Virulence Associated Factors For Coagulase - Negative Staphylococci Isolated From Urinary Tract Infection In Babylon Province

Author name: سارة هاشم داخل الحلي
Supervisor name: محمد صبري عبد الرزاق السعيد | جواد كاظم الخفاجي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: يهدف هذا البحث الى دراسة بكتريا المكورات العنقودية السلبية التخثر, وشملت الدراسة جمع120 عينة ادرار من المرضى المصابين بالتهاب المجاري البولية من الاشخاص المراجعين والراقدين في مستشفى بابل للنسائية والاطفال ومستشفى الحلة التعليمي وللفترة من كانون الاول 201 | This work aims to study coagulase - negative Staphylococci, which included 120 urine sample were obtained from patients suffering from urinary tract infection; who attained to Babylon Maternity and Pediatrics Hospital, and Al - Hilla General Teaching Hospital, during the period from December 2015 to March 2016), they were diagnosed as having UTI by the Urologists. The samples were analyzed for any signs of infection and immediately inoculated on blood and MacConkey agar media plates. All plates were incubated aerobically at 37 ?C for 24 - 48 hrs.Results of morphological and biochemical characterization revealed that out of total of 120 urine samples, 110 showed positive culture, among them 37 isolates are belonged to coagulase - negative Staphylococci, other 73 isolates belonged to other bacterial genera.Molecular detection of coagulase gene (coa) was done for all CoNS isolates. The results showed that 28/37 isolates (75.67%) were positive for this marker, distributed as follows : S. epidermidis gave 15 isolates, S. saprophyticus gave 9 isolates and S. haemolyticus gave 4 isolates.Also, molecular investigation of fnb A and B genes are carried out for all CoNS isolates by using specific PCR markers. 13/37 isolates (35%) were positive for fnb A gene, as 6/13 of S. epidermidis, 5/13 of S.saprophyticus and 2/13 of S. haemolyticus have this gene in their genome. On the other hand, only 4/37 isolates gave positive result for fnb B gene; as 2 isolates of both S. saprophyticus and S. epidermidis have this gene in their genome.Moreover, sec gene also was investigated in CoNS isolates. The results showed that all CoNS isolates are free from this gene.Regarding urease enzyme production, S. epidermidis and S.saprophyticus isolates were found to be urease positive, while S.haemolyticus isolates display urease negative.To demonstrate the ability of bacteria to form struvite stone, urine samples obtained from healthy individuals were used. It was found that struvite stone was formed by S. epidermidis and S. saprophyticus isolates.Moreover, susceptibility to 10 antibiotics were tested by using disc diffusion test (DDT). S. epidermidis was showed 100% resistance for each Methicilline, Ceftriaxone, Ceftazidime and Cefoxitin. Also, it show high resistance 94%, 88%, 88%, 76%, and 59% for Impenem, Gentamycin, Pipracillin, Ciprofloxacin and Amikacin respectively. However, S. epidermidis showed low resistance (6%) for Vancomycin. So, all S. saprophyticus isolates showed high resistance (100%) to each Pipracilline, Ceftriaxone, Methicillin, Ceftazidime and Ciprofloxacin. It showed high resistance (92%) for each Vancomycin and Imipenem, and (83%), (75%), (75%) for Cefoxitin, Gentamycin and Amikacin respectively. In addition to that, all S. haemolyticus was showed (100%) resistance for each Ceftriaxone, Pipracillin, Methicilline, Ceftazidime and Cefoxitin. Also, showed high resistance (75%), (63%) and (50%) for Gentamycin, Amikacin and Ciprofloxacin respectively. While it was showed low resistance (13%) to Vancomycin.

الصورة البكتيرية والنسيجية المرافقة لالتهاب اللوزتين المزمن والادينويد عند الاطفال == Bacteriological And Histopathological Profile Associated With Chronic Tonsillitis And Adenoid Hypertrophy In Children

Author name: رشا محسن كاظم الحسيني
Supervisor name: حبيب صاحب نهر | صفاء حسين الطريحي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: This study aimed to investigate the bacterial profile of surface and core of infected tonsils and adenoid tissues and histopathological examination of these organs.A total of 122 samples represented by 63 excised tonsils and 59 excised adenoids in addition to 57 blood samples were collected from 70 patients who were referred to Hilla Teaching Hospital (Ear, Nose and Throat unit) in Hilla city within a period of four months from November 2015 to February 2016. Those patients weresuffering from chronic tonsillitis and adenoid hypertrophy. The ages of those patients ranged from 2 to 15 years, 41 (59%) of them were males and 29 (41%) were females.The study included three main parts. The first one was the bacterial diagnosis based on relied diagnostic procedures. Second part was the detection of antistreptolysin O (ASO) by using latex agglutination test, while the third part included histopathological study to detect the pathological lesions that occur during these infections.The results indicated that, the most age group being susceptible for chronic tonsillitis and adenoid hypertrophy was the group of 7 - 10 years, represented 33 patients with a percentage of (47%). Among this group, males were more susceptible with percentage of 20 (29%) compared to female patients13 (19%).The surface of all samples (tonsil and adenoid) revealed positive results for bacterial culture, while in the core of tonsil only 44 samples (70%) were positive for bacterial culture while 19 samples (30%) were negative results (no growth). As in tonsils, the core of adenoid revealed positive results in 32 (54%) samples, while 27 (46%) samples were negative results.The suspected isolates were fully identified by using bacteriological, biochemical and Vitek 2 techniques (for two isolates because of those isolates give variable biochemical test results which confused their diagnosis that lead to using of vitek system technique to diagnosis them, these isolates were rare bacteria that infect tonsil and adenoid). The most common types of bacterial isolates were Staphylococcus aureus which accounted for 75 isolates (23.6%), followed by Streptococcus pyogenes 61isolates (19%), Escherichia coli 57 isolates (18%), Klebsiellae pneumoniae 55 isolates (17.3%), Hemophilus influenza 37 isolates (11.6%), Sphingomonas paucimobilis 14 isolates (4.4%), Staphylococcus epidermides 7 isolates (2.2%), Pseudomonas aeruginosae 6 isolates (2%), Pseudomonas stutzeri 5 isolates (1.6%), and Staphylococcus saprophyticus only one isolate (0.3%).Regarding the bacterial population, there is a difference between tonsils / adenoid surface and tonsil/ adenoid core culture were the most predominant isolate from tonsil surface was Staphylococcus aureus and K. pneumoniae from tonsillar core, while from adenoid surface was Streptococcus pyogenes whereas E. coli from adenoid core.The ASO titer in patients with or without group A streptococcal infections were determined by using latex agglutination test. The results revealed that 8 samples (14%) exhibited positive results, while 49 samples (86%) exhibited negative results out of 57 samples. The results showed statistically no significant differences between patient age groups but showed highly significant differences between males and females.Eight specimens were taking for histopathological examination randomly, four samples were adenoids and four samples were tonsils. The histopathology examination of tonsil and adenoid showed the presence of lymphoid hyperplasia,multiple lymphoid follicles, vascular congestion, fibrosis, germinal centers, infiltration of inflammatory cells, necrosis and apoptosis of tissues

تقييم المؤشرات السريرية وبعض الدلائل الحيوية في مرض دواعم الاسنان == Evaluation Of Clinical Parameters And Some Biomarkers In Periodontal Disease

Author name: امل عليوي حسن
Supervisor name: سعد مرزة حسين الاعرجي | وسام وهاب الحمادي
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: امراض دواعم الاسنان هي مجموعة من امراض الالتهابات التي تصيب التجويف الفموي وتعرض الهياكل الداعمة للاسنان للخطر. تحدث هذه الامراض بسبب عدم التوازن بين الجراثيم المحيطة بالاسنان واستجابة المضيف. يعتبر مرض التهاب اللثة ومرض النساغ المزمن هي من الاقسام الرئيس | Periodontal diseases are a group of inflammatory diseases that affect the oral cavity and jeopardize the supporting structures of teeth. They are caused by an imbalance between periodontal pathogens and host responses. Gingivitis and chronic periodontitis are the major categories of these diseases. The study sample consisted of 80 individual of both genders, their age ranged from 30 to 50 years, and they are divided into three main groups according to the clinical periodontal parameters (plaque index, gingival index, bleeding on probing index, probing pocket depth index and clinical attachment level index). Group ? comprised from 24 (15 female and 9 male) healthy gingival volunteers as a control, group ?? comprised from of 29 (12 female and 17 male) patients with gingivitis, lastly group ??? comprised from 27 (11 female and 16 male) patients with chronic periodontitis. Unstimulated whole saliva samples are collected to determine the levels of Prostaglandin E2, Matrix Metalloprotinase - 8 and Alkaline Phosphatase. Clinical periodontal parameters are recorded at four sites per tooth. The results of present study revealed a highly significant difference (P - value < 0.01) in the mean ± SD for all of clinical periodontal parameters and for the levels of salivary biomarkers (Prostaglandin E2, Matrix Metalloproteinasee - 8 and Alkaline Phosphatase) among chronic periodontitis, gingivitis and control groups. The chronic periodontitis group revealed the higher mean value followed by the gingivitis group and the control group presented the least value. Regarding the correlation between the salivary biomarkers levels and clinical periodontal parameters, the present study showed a significant positive correlation (P< 0.05) and a highly significant positive correlation (P - value < 0.01) between each of salivary biomarker levels and all of clinical periodontal parameters. In addition, this study showed a highly significant positive correlation (P - value < 0.01) between Prostaglandin E2 and Matrix Metalloproteinasee - 8 and a significant positive correlation (P< 0.05) between Prostaglandin E2 and Alkaline Phosphatase and between Matrix Metalloproteinasee - 8 and Alkaline Phosphatase for gingivitis and chronic periodontitis groups. The present study re - emphasized the importance of whole saliva as sampling method in relations of immunological purposes in gingivitis and chronic periodontitis and proposed that the elevated levels of Prostaglandin E2, Matrix Metalloproteinasee - 8 and Alkaline Phosphatase may be one of the host - response mechanisms connect with the clinical appearances of periodontal diseases. Salivary Prostaglandin E2, Matrix Metalloproteinasee - 8 and Alkaline Phosphatase levels may reflected the severity of disease, thus help in the early detection of the disease.

تاثير احمرار الدم الثانوي الدم على وظيفة بطانة الاوعية الدموية الجهازية في محافظة بابل == Effect Of Secondary Polycythemia On Systemic Endothelial Function In Babylon Province

Author name: احمد محي الدين سهراب
Supervisor name: غافل صيهود حسن الشجيري | عامر صاحب المؤمن
General topic: Medicine
Specific topic: Physiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Physiology And Medical Physics
Language: English
University location: Babylon
First pages:
Abstract: تغير قطر الشريان العضدي كاستجابة الى الاحتقان , يستعمل بصورة واسعة لتقيم وظيفة بطانة الاوعية الدموية الجهازية بواسطة تسجيل التغير كاستجابة للاحتقان. ضعف او قلة التوسع يلاحظ بصورة واضحة لدى المرضى المصابون باحمرار الدم الثانوي.التوسع المحدث بالمد يقاس كن | Brachial artery diameter variations in response to hyperemia were widely used for the assess endothelial function by recording variations in response to hyperemia. Vasodilation was markedly impaired in polycythemic patients.Flow mediated dilatation (FMD) was measured as the percentage increase in brachial artery diameter at diastole after 5 minute of ischemia.Objectives : Material and MethodsThe present study was conducted at Echo - Doppler department in Marjan medical city / Hilla , in cooperation with the department of physiology of College of medicine / University of Babylon, from November 2015 to April 2016. A history, physical examination, blood pressure, and laboratory were performed on all subjects (patients with secondary polycythemia, and control groups). Seventy subjects were included in the present study. Their ages was ranged between 40 - 60 years. They were divided into two groups. Group 1 : Included 30 healthy subjects without polycythemia, all men, their mean age was 47.20 ± 7.3 years and they served as control group.Group 2 : Included 40 patients, all men, with secondary polycythemia, their mean age 46.90 ± 7.3 years.Method : Measurement of flow - mediated and nitroglycerin - induced dilatation of the brachial artery.Measuring blood pressure, auscultatory method.Assessment of BMI.Laboratory analyses : Serum urea test.Serum creatinin test.White blood cells (WBCs) count : Platelets count.Estimation of packed cell volume (PCV %).Red blood cells (RBCs) count. Hb. level test.Cholesterol test.Triglyceride test.Urea test.Creatinine test.Results : The mean flow mediated dilatation (FMD) on two groups was significantly (p value < 0.01) decreased (16%) in patients group (8%) comparison to control group (24%). The mean Hb concentration was significantly (P value < 0.01) increased in patients group compared to control group.The mean packed cell volume (PCV) was significantly (P vale < 0.01) increased in patients group compared to control group.The mean FMD was found to be significantly (16%) decreased in smoker patients group (8%) compared to nonsmoker control group (24 %).The mean red blood cells count was significantly (P value < 0.01) increased in patients group compared to control group.It was found that triglyceride level significantly increased (P value < 0.01) in patients group compared to control group.There was negative correlation between triglyceride and FMD in patients group. Their are No correlation between age and FMD in patients group.The mean serum cholesterol level was not significantly ( p value < 0.07) increased in patients group compared to control group , while serum triglyceride level, serum creatinin, serum urea were significantly ( p value < 0.01) increased respect in patients comparison to control. Mean glucose, Platelets and red blood cells counts were significantly (P value < 0.01) increased in patients group (polycythemic group) respectively compare to control group respectively.Mean body mass index (BMI) and packed cell volume (PCV) were significant (P value < 0.01) increased respectively in patients group compared to control group. Ages of patients group was non - significantly (P value = 0.89) decreased (46.90 ± 7.00 years) in patients group compare to control group.Conclusions : 1 - The mean flow mediated dilatation (FMD) was significantly decreased (16%) in patients group compared to control group. 2 - The mean Hb concentration , mean packed cell volume (PCV) , red blood cells count, platelets , and body mass index count was significantly increased in patients group compared to control group.3 - Polycythemia as with endothelial dysfunctions that may perform in diagnosis of arterial disease. That used to estimate whereas endothelial dysfunctions result from problem in the cardiovascular system with problem in the prothrombin time.4 - Polycythemia can result in raises causes of thrombosis.Through increase platelet dysfunctions, increase ; white blood cell counts, Hb count, homocystein and cholesterol level as with endothelial dysfunctions.

التاثير الموضعي لعلاج الفيناسترايد في علاج مرض الشعرانية مجهولة السبب == The Effect of Topical Finasteride In Treatment of Idiopathic Hirsutism

Author name: يحيى ابراهيم يحيى
Supervisor name: نسرين جلال محمد البياتي | وسام علي امين
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Medicine
Language: English
University location: Babylon
First pages:
Abstract: يعرف مصطلح الشعرانية هو الشعر الاكثر خشونة وسمكا في النساء مثل نمط والمواقع في الذكور , الاندروجين المسؤولة عن تغير في الصوت وزيادة في كتلة العضلات في المراة هو هرمون التستوستيرون، وانه المسؤول عن الشعرانية، ونظرا لحدوث درجة عالية من هذا المرض في العراق | The term hirsutism defines as presence of coarser, thicker and terminal hair in women in a male like pattern and locations.The androgen responsible for the change in voice and the increase in muscle mass in women is testosterone, and that responsible for hirsutism and due to the highly incidence of this disease in Iraq so the finasteride cream 1% used and the study was carried out in Collage of Medicine / Babylon University from November 2013 - November 2014 for treatment of fifty five females were enrolled in this study. Their age was between (18 - 55 years) and the mean of their age was 32.26. Those females were chosen from Consulting dermatologist department in Mergan medical city in Babylon in which these females were complained from hirsutism and After preparation Finasteride 1% cream and using it by the patients it was measured TSH,FSH,LH and free Testosterone and the follow up every 15 day and after 3 months (end of study) it was measured the same parameters TSH,FSH,LH and free Testosterone and ferryman gallawy scores the patients had two parts : (Pretreatment) the patients with TSH,FSH,LH and free Testosterone level before treated with finasteride cream. (Post treatment) the patients were taking the Finasteride cream and the TSH,FSH,LH and free Testosterone levels measured after three months of treatment with finasteride cream and also measured the : • Hair color• Hair removal frequency of the patients• Pain severity before and after treatment• F - Gallawy score• Visual analogue scoreand the follow up every 15 day and after 3 months (end of study) it was measured the same parameters Finasteride cream 1% had significant decrease in the serum free testosterone levels with no significant effects on others parameters as TSH and FSH and LH level. From the above results we can conclude the following : Finasteride cream 1% is an effective and harmless treatment in patients whom suffer from idiopathic hirsutism.

التشخيص الجزيئي لتجرثم الدم لدى الاطفال حديثي الولادة بواسطة استخدام تفاعلات البلمرة المتسلسل في مدينة الحلة == Molecular Detection of Neonatal Sepsis By Using PCR In Hilla City

Author name: نور حامد عباس المرزوك
Supervisor name: الهام عباس بنيان الساعدي | ميساء صالح الشكري
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language: English
University location: Babylon
First pages:
Abstract: في هذه الدراسة, تم جمع 85 عينة دم من 85 مريض مصابين بتسمم الدم (حسب التشخيص السريري الاولي للطبيب), تتراوح اعمارهم بين 1 - 28 يوم من كلا الجنسين (الذكور 53 والاناث 32) للفترة من تشرين الثاني 2014 الى اذار 2015.توضح نتائج هذه الدراسة ان (52,9%) طفلا حديث | Neonatal sepsis can be defined as any systemic bacterial infection confirmed by a positive blood culture in the first month of life. In this study, (85) blood specimens were collected from (85) patients with suspected septicemia, age ranged between 1to 28 days from both sexes (53 males and 32 females) during the period from November 2014 to March 2015.The results of this study revealed that (52.9%) are presented with late onset sepsis (infected during 7 - 28 days of their life), while the rest (47.1%) of the neonates are presented with early onset sepsis (infected during the first seven days of their life). The collected samples were investigated for bacterial isolation. Bacterial culture was positive in (7.06%) patients versus (92.9%) patients revealed a negative bacterial culture. The most common types of bacteria isolated were three isolates of Klebsiella spp. (50.0%), followed by one isolate Staphylococcus aureus (16.67%), one isolate E. coli (16.67%), and one isolate Enterobacter spp. (16.67%).The virulence factors of some bacterial isolates in this study were studied. The capsule was present in all bacteria isolated in this study (100%) except for Enterobacter spp. which did not possess the capsule.Colonization factor antigens were detected in all isolates. It was found that only Klebsiella spp. and E. coli (100%) have the ability to produce CFAI and CFAIII. Whereas Enterobacter spp. and Staphylococcus aureus (100%) have the ability to produce colonization factor antigen I only. The results also showed that all isolates of Klebsiella spp. and Enterobacter spp. did not produce hemolysin, while all isolates of Staphylococcus aureus and E. coli were able to produce hemolysin.Extracellular protease was also investigated. It was found that (100%) of all bacteria isolated in this study were able to produce extracellular protease.Biofilm formation was investigated in all bacterial species, and the results showed that all isolates of Gram negative bacteria and Gram positive bacteria form a biofilm.The ability of some bacteria to produce bacteriocin was tested and the results showed that E. coli and Klebsiella spp. were able to produce bacteriocin.In - vitro, the susceptibility of bacteria towards some antibiotics included in this study revealed that the amikacin and kanamycin are the most effective antibiotics to different types of pathogenic bacteria. Finally, molecular detection for bacterial sepsis was done by using specific primers (16 sRNA, rpoB and its). It was found that 16 sRNA genes were present in (20%) of samples and rpoB gene present in (18.8%). While its gene used for detection of fungi revealed negative results in all samples.
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