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تقييم خليط الكيتامين والترامادول والباراسيتامول والزايلازين كبرامج للتخدير العام بالحقن في الاغنام == Evaluating of Ketamine, Tramadol, Paracetamol And Xylazine Combination As An Injectable General Anesthetic Protocols In Sheep

اسم المؤلف: علي اسماعيل جاسم
اسم المشرف: ثاير علوان عبد
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الجراحة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسة الى تقييم استخدام الكيتامين (K)، والترامادول (T)، والباراسيتامول (P) والزيلازين (X) كبرامج تخدير للاغنام باستخدام جرعات مختلفة وطرق اعطاء مختلفة. استخدم في التجربة ثلاثون من الاغنام الكبار البالغة من السلالة المحلية (تراوحت معدل اوزانها 27.4 | The study aim to evaluate the use of ketamine (K), tramadol (T), paracetamol (P) and xylazine (X) as an anesthetic protocol for anesthesia in sheep by using different doses and different routes of administration. Thirty animals of adult local breed sheep weighing (27.4±2.46) kg were used in 6 different anesthetic protocols. The animals were divided into six groups (5 animals of each). The first three groups (without using of xylazine) were giving the drugs by IV injection in the jugular vein : G1 giving K4 and P10 mg/kg IV, G2 giving K4T2 mg/kg IV, and G3 giving K4 T2 P10 IV. In the other groups (G4, G5, and G6) xylazine were adding to the anesthetic protocol. G4 giving K4 T2 P10 X0.05 by IV route. G5 was giving K10 T2 P10 X1 mg/kg by IM route and G6 giving K20 T4 P10 X2.5 mg/kg by IM route. The HR, RR, RT, muscle relaxation, limb, and flank analgesia, and rumen movement were taken before administration of anesthesia (time 0) and consider as the control reading. Then taken at 5, 10, 15, 20, 30, 45 and 60 minutes or till the end of anesthesia. In the IV groups (without using of xylazine), (G1, G2, and G3). The induction time was 53.2, 60, and 68 seconds, the surgical anesthesia was 2.8, 5, and 5 minutes, and the total recovery time was 4.8, 5.4, and 3 minutes in G1, G2 and G3 respectivily. The RR, and HR were increased, and the RT was decreased. The muscle relaxation was mild, and the analgesia was moderate. In G4 after adding of xylazine and giving the anesthetic protocol by IV route, the induction time, the surgical anesthesia, and the total recovery time were become 50.6 seconds, 23 minutes, and 9.4 minutes respectively. The HR, RR, and RT were increased, the muscle relaxation, and analgesia were moderate. In G5 after increased the dose of ketamine and xylazine and giving the anesthetic protocol by IM route, the induction time, the surgical anesthesia, and the total recovery time were 5, 36, and 5.6 minutes respectively. The HR and RR were decreased, and the RT was increased. The muscle relaxation was deep for 10 minutes, and the analgesia was moderate for 10 minutes also. In G6 after doubling the ketamine, tramadol, and xylazine doses, and giving the anesthetic protocol by IM route. The induction time, the surgical anesthesia, and the total recovery time were 3.6, 73.4, and 7 minutes respectively. The HR started stable, then after 15 minutes decreased sharply, The RR was irregular, with apnea, the RT was increased, the muscle relaxation was deep for 35 minutes, and the analgesia was deep for 45 minutes. In conclusion the addition of tramadol and paracetamol to the ketamine anesthesia of sheep in low doses, improve the induction and recovery time of anesthesia, without increase the duration and depth of anesthesia. Adding of xylazine to the anesthetic protocols increase the duration and depth of anesthesia. The protocol of K10 T2 P10 X1 by the IM route is the best protocol dose among the six protocols used in this study.

Coccidiosis In Poultry

اسم المؤلف: يثرب خضر عبيس
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تعتبر الكوكسيديا في الدواجن واحدة من الامراض الرئيسية التي تؤثر على اداء الدواجن المرباة تحت نظام الانتاج المكثف الذي يؤثر على صناعة الدواجن في جميع انحاء العالم، بخسائر اقتصادية كبيرة عن طريق قلة التحويل الغذائي وخفض الانتاجية. هذا ليس فقط يعيق نمو الدجا | Coccidiosis in poultry is still considered as one of the main diseases affecting performance of poultry reared under intensive production system that affects the poultry industry worldwide , having major economic losses in poultry by reducing performance and decreasing productivity.this disease not only hinders the growth of chickens but also facilitates other epidemic diseases. Coccidiosis is mainly controld by prophylactic coccidioststs administrated in the feed. However , the extensive use of these drugs has resulted in the development of drug resistance by Eimeria spp., Which causes Coccidiosis.The aim of the study was to updated the recent information of of infection,gross evaluation of the intestinal tract and microscopic evaluation of wet smear are used as routine diagnostic methods and to acquire data on the prevalence of coccidiosis and drug resistance of field isolated in chickens,Frequent use of anticoccidial drugs ,however , has resulted in the development of resistance in the Eimerian spp. Because farmers mainly rely on the prophylactic and therapeutic use of chemicals for the control of avian coccidiosis. Increasing awareness about populic health hazards associated with drug residues in food chain has also added to the constraints in using synthetic drugs for treatment and control of disease in animals and control methods according to the collected formation chickens were in danger of clinical coccidiosis , and here coccidia generated a certine degree of resistance and not easy ty control in our industry in Iraq because of that the poultry industry in Iraq is not as integrated system without strict biosecurity. The study also highlight the information on the anticoccidial vaccines are in the market with controversial efficacy.

تشخيص فايروس التهاب القصبات المعدي في حقول دجاج اللحم في محافظة ذي قار باستخدام تقنية الاليزا (ELISA) وتفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) == Identification of Infectious Bronchitis Virus In Thi - Qar Province In Broilers Farms By Using ELISA and rRT - PCR

اسم المؤلف: ظافر ياسر عزيز الفاضلي
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لتقييم مدى انتشار مرض التهاب القصبات المعدي الفيروسي في دجاج اللحم في محافظة ذي قار, قسمت منطقة الدراسة جغرافيا الى خمسة مناطق (شمالية, جنوبية, غربية , شرقية, مركزية) حيث تمت الدراسة على عشرون حقل دجاج لحم غير ملقحة اربعة قاعات من كل منط | The current study was conducted for evaluation of prevalence of IB in broilers in the Thi - Qar province, which geographically divided into five regions, (Northern, Southern, Western, Eastern and central area), twenty broiler farms without history of previous vaccination, four from each region were included in this study. Serum and autopsies were collected from affected birds after recording of the clinical signs that observed on the birds; positive serum samples that detected by rapid test were used for evaluation the antibodies titrations by ELISA technique, while the histological specimens (trachea, lung and kidney) were used for detection of histopathological changes and detection of presence of the IBV infection by molecular approach (real time - PCR). The clinical manifestation includes : typical respiratory sings of IB infections, with depression, rough feather, and assembling near the heat source, as well as some birds were found dead with mortality rate ranging between 30 - 80%. Also 96 serum samples were collected from other broiler farms as following ( 42 asymptomatic vaccinated, 54 asymptomatic non - vaccinated, 26 symptomatic vaccinated) for ELISA and real time PCR tests. The result of rapid test showed that the percentages were different among the areas of study when the southern area revealed the highest percentage (80%) among the others followed by the northern area and western (70%), then central and eastern(65%). The results of ELISA showed a high titer for antibody of IBV in all these farms in areas of the study, but the highly significant results (P<0.05) were found in the southern area(7032.2±1640.92) and then northern area (5722.18±2060.1), a less significant differences were in central and eastern areas (3188.11±742.45; 3161.8±52.6) respectively. In relation to age, the high titer of IB antibodies in total 20 farm by ELISA were recorded in 12 farms at 4 weeks of age (60%) followed by 4 farms at 5 weeks of age (20%), 3 farms at 3 weeks of age (15%) and 1 farm at 2 weeks of age (5%), while there is no recorded infection in farms at 6 ages. According to history of vaccination and clinical manifestation of tested birds, most of the tested flocks showed high level of antibody titers to IBV by ELISA technique in asymptomatic vaccinated chickens samples 38/42 (90%) followed by asymptomatic non vaccinated chickens 8 (14.8%) and symptomatic vaccinated chickens 6(23.07%). While rRT - PCR showed that 69 ( 80%) sample were revealed positive results. The most important gross lesion observed on immediately the necropsy after death were tracheal congestion, caseous exudates in the trachea, pneumonia, airsacculitis, pericarditis and perihepatitis, as well as, nephritis; while The histopathological changes were summarized by desquamation of tracheal epithelial cells and hemorrhage with infiltration of inflammatory cells specially lymphocyte cells, hyperplasia of epithelial cells with hemorrhage, lung sections showed severe infiltration of inflammatory cells mostly lymphocyte cells and severe congestion with spots of pneumonia and the histopathological changes in kidneys were characterized by tubules degeneration , glomerular distention with severe hemorrhage and lymphocyte infiltration.

تركيز المعادن الثقيلة في حليب الابقار والاغنام والماعز في محافظـة القادسية == The Concentration of Heavy Metals In Milk of Cows , Sheep & Goats In Al - Qadisiya Province

اسم المؤلف: علياء حسن علـي السماوي
اسم المشرف: كريم ناصر طاهر
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الصحة العامة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في محافظة القادسية للمدة من كانون الاول 2012 لغاية اذار 2013 شملت خمسة مناطق مختلفة من محافظة القادسية هي كل من (مركز الديوانية وناحية السنية وناحية الشافعية وناحية الدغارة وقضاء الحمزة) لغرض تحديد مدى تلوث الحليب الخام ببعض المعادن | This study was carried out in AL - Qadissiyah province during the period from December 2012 to March 2013 involved five different regions such as (Diwaniyah city center , Saniyah district , shafeia district , Dagharah district and AL - Hamza district) to determine the level of contamination of raw milk with the toxic heavy metals such as Cadmium (Cd), Lead (Pb), Nikel (Ni), Arsenic (As) and Mercury (Hg). A total of 150 raw milk samples were collected from 150 animals belonged to three species involved (50) cows , (50) sheep and (50) goats selected randomly, ten animals per each species per regions. The results of a statistical analysis of the experimental data showed that : Chemical components of milk 1.There are a significant differences (p <0.05) in all contents of milk among different species of studied animals (cows , sheep and goats). Except fat content for cows and goats milk were not significant different. Sheep's milk had significantly (p<0.05) of (protein, fat, solids not - fat and ash) in comparison to cow's and goat's milk (7.535, 6.617, 12.026 and 0.646) % respectively. Cow's milk had significantly (p<0.05) higher lactose content (4.757%) than both sheep's and goat's milk. components of milk 2. PhysicalThe results revealed that there were significant differences (p <0.05) in all physical characteristics of milk among different species of animals involved in this study (cows, sheep and goats). Sheep milk had significantly (p<0.05) (Titratable Acidity 0.182% and conductivity 3.214 mS/cm) than both cow's and goat's milk , goat's milk contains the highest specific gravity 1.057mg /100 ml while the pH values of cow's , sheep's and goat's milk were similar. Heavy metals in milk - Effect of Species The results revealed that significant differences (P<0.05) among the mean values of heavy metal concentrations included in the study (Cadmium (Cd) , lead (Pb), Nikel (Ni), Arsenic (As) and Mercury (Hg) in milk of different species (cows , sheep and goats). Sheep's milk had significantly levels of such heavy metals (0.251, 0.801, 0.563, 0.283 and 0.124) mg/ l respectively than both cow's and goat's milk while cow's milk had significantly the lower levels (0.098, 0.311, 0.221, and 0.122 , 0.037) mg / l respectively. - Effect of regions : The results showed that significant differences (P<0.05) in the means values of heavy metal concentrations in milk among different regions. The Highest levels of heavy metals were observed in AL - Hamza district (0.184, 0.624, 0.494, 0.246 and 0.119) mg / l respectively and the lowest levels of heavy metals were noticed in Dagharah (0.144 , 0.507, 0.382 , 0.167 and 0.061) mg / l respectively. - Effect of month : The results of the statistical analysis showed that there were non - significant differences in the average concentrations of cadmium, lead and arsenic among months while a significant differences (P <0.05) were observed in the level of nikel between march and other studied months [(0.494) mg / l in march and (0.397, 0.418 and 0.415) mg / l for December, January and February respectively]. Also the results showed that there were a significant differences (P<0.05) in the levels of mercury between march and each of January and February [(0.106) mg / l in march and (0.081 and 0.083) mg / l in January and February respectively]. From the results obtained in the present study,it can be concluded that : 1 - Heavy metals like cadmium (Cd), lead (Pb), Nikel (Ni), Arsenic (As and Mercury (Hg) which distributed among different sites were exceed the maximum acceptable limits set by WHO and EPA. 2 - High concentration of heavy metals (Cd, Pb, Ni, As and Hg) in city center site may be due to the industrial activities. 3 - High concentration of heavy metals in the other sites may be due to application of waste water for agriculture purposes (irrigation) which pollute the soil and also be allowing toxic metals to enter in to the food chain posing a serious health hazard.

التحري عن اهم الامراض الفيروسية التنفسية في دجاج اللحم باستخدام فحص الاليزا (ELISA) وتقنية تفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) في محافظة الديوانية == Detection of Certain Viral Respiratory Diseases In Broiler Chickens By ELISA And Real Time RT - PCR Technique In Al - Diwaniyia Province

اسم المؤلف: عباس هادي جاسم المحمودي
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تعد انفلونزا الطيور والتهاب القصبات المعدي والنيوكاسل من الامراض المهمة اقتصاديا في صناعة الدواجن.صممت الدراسة الحالية لتسليط الضوء على دور فيروسات ND, IB, AI , ومعرفة مدى انتشارها في احداث الاوبئة التنفسية في دجاج اللحم باستخدام تقنية الاستنساخ المن | Avian influenza, Newcastle disease and Infectious bronchitis are diseases that have economical importance in poultry industry. This study was designed to clarify the roles of IBV, AIV and NDV in an outbreaks of respiratory diseases in broiler chickens by using ELISA and real time RT - PCR assay in Al - Diwaniyia province , Iraq. A total of 30 commercial broiler flocks with high mortality (20 to 80 %) in Al - Diwaniyia province were investigated.Tracheal swabs and tissue specimens ( Trachea, lung, kidney and cecal tonsils ) were tested initially by rapid test for detection of Infectious bronchitis virus ( IBV ) , Influenza type A virus ( AIV) and Newcastle disease virus (NDV).The total results of rapid immunochromatography assay showed that out of 30 flocks 28 ( 93.33%) were positive for IBV and 21(70%) flocks were positive for AIV while 4(13.33%) flocks were positive for NDV. Blood samples were collected for detection of antibodies against IBV, AIV & NDV in serum samples , the results of indirect ELISA showed that out of 30 flocks were 30(100%) gave positive result with indirect ELISA test for IBV and 23(76.67%) flocks were positive for AIV (H9 ) whereas 5(16.67%) flocks showed positive result for NDV. The result of rRT - PCR showed out of 30 (100%) flocks were positive for IBV and 23(76.67%) positive for AIV (H9) whereas 5(16.67%) flocks were gave positive results for NDV. In conclusion the high positivity in the examined broiler flocks were mainly due to mixed infections of IBV, AIV(H9) and NDV, However we concluded that IBV and AIV were most important causes of respiratory diseases in this study. Also this study demonstrated that Real time qRT - PCR technique and ELISA were rapid and accurate as diagnostic tool in early detection of IBV, AIV and NDV.

التهاب الامعاء التنخري في الدواجن الامراضية, وطرق السيطرة والعلاج == Necrotic Enteritis In Poulry Pathogenesis, Prevention And Curative Measures

اسم المؤلف: سهى نجم الربيعي
اسم المشرف: علاء عبد العزيز عبد
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

الكشف الجزيئي والمصلي لداء المقوسات في الانسان والاغنام في محافظة واسط == Molecular And Serological Detection of Toxoplasma Gondii In Human And Sheep In Wasit Province

اسم المؤلف: عباس حسن خلاطي السراي
اسم المشرف: نعمان ناجي عايز
الموضوع العام: الطب البيطري
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحاليه خلال الفترة من تشرين الاول 2013 الى ايار 2014 في محافظة واسط للكشف عن الاصابة T. gondii في الانسان والاغنام باستخدام الطرق الجزيئية والمصلية. تم جمع خمس مئة وثمانية عينات 500 عينه دم و8عينات من انسجه المشائم و305 عينه 300عينه دم و5ع | The present study was conducted during the period from October 2013 to May 2014 in Wasit province to detect the infection of T. gondii in women and sheep using Molecular and Serological methods.Five hundred and eight samples 500 blood samples and 8placenta and 305samples 300 blood samples and 5placenta were collected from both suspected women and sheep respectively. The sera samples were separated and examined by ELISA for human and Latex agglutination test for sheep to detect the infection with T. gondii serologically, then many blood and placental tissue samples (89 blood samples and 8 placental tissue samples for women, whereas 100 blood samples and 5 placental tissue samples for ewes) were subjected to polymerase chain reaction (PCR) technique to detect the infection molecularly.The Serological results showed that 17.8% of women were positive for the private screening ELISA detects toxoplasmosis (17 % with chronic infection and 0.8% with acute infection), it was noted that the highest rate of infection was in women who ranged in age between 20 - 29 years, reaching 19.9%, but with no significant difference (P>0.05) between the ages studied. On the other hand the rate of sheep infection by latex agglutination test with 2 - mercaptoethanol amounted to 33.33% (31.33% with chronic infection and 2% with acute infection) and most positive cases were among the ages which were equal to or more than 3 years (?3 years) with significant difference (P<0.05) between these ages. The present study indicated a lack of months effect on the distribution of parasite infection rates where these different months recorded relatively close rate ranged between 14.45% - 23.07% in women and 31.42% - 35.97% in sheep with no significant difference (P>0.05).Regarding to polymerase chain reaction test, a fragment of 399bp was amplified from B1 gene, the result showed that 6.74% and 4% of blood samples and 100% and 80% of placental tissue samples which taken from women and sheep respectively were positive to this test.

تحديد القرابة الوراثية لعزلات عراقية ضارية لفيروس مرض النيوكاســــل من تتابع القواعد النتروجينية في موروث الهموكلوتنين نيورامنيديز == Phylogenetic Determination of Newcastle Disease Virus Hn Gene Sequences Iraqi Virulent Isolate

اسم المؤلف: مرتضى عبد المهدي محمد حسن المظفر
اسم المشرف: هدى عبد الهادي علي النصراوي | احمد مجيد حمزة الشمري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض المشتركة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: يعد مرض النيوكاسل من اخطر الامراض الفيروسية المعدية التي تصيب قطعان الدواجن وتسبب خسائر اقتصادية كبيرة في صناعة الدواجن واول تسجيل لمرض النيوكاسل كمرض مشترك بين الانسان والحيوان كان من قبل العالمBurnet في سنة 1943. والهدف من الدراسة هو عزل وتحليل تسل | Newcastle disease is considered to be the most contagious poultry disease and may cause severe economic loss in the poultry industry. The first report in which Newcastle disease virus (NDV) was consider as zoonotic disease and a human pathogen that was published by Burnet, in 1943. The aim of this study was to isolate and determination of nucleotide sequence of the Hemagglutinin - neuraminidase gene (HN) of two NDV Iraqi virulent isolates. These isolates were named as Najaf isolate (Najaf APMV1/ Chicken/ Iraq - Najaf/ ICCMGR/2012) and Baghdad isolate (Baghdad ICCMGR). This work was conducted in Iraq for the first time, the reason behind it was to correlate phylogenetically between these two isolates and the NDV strains in the countries of the region. This will help in determining the source of NDV outbreaks occurred in Iraq. Both Najaf and Baghdad isolates were propagated in embryonated chicken eggs, after inoculation of 11 days embryonated chicken eggs was showed and embryos were killed in different times (more than 40 hours and less than 72 hours). To assess the activity of both virus isolates, the main features of the infected embryos that reflect characteristics of congestion and sever hemorrhage was compared with control one. Hemorrhage was markedly severed in the infected embryos and it was similar in its severity by both isolates. The allantoic fluid was harvested post infection and the agglutination activity reflected titer of 1024 for Najaf isolate and 512 for Baghdad isolate. In order to isolate the Hemagglutinin - neuraminidase gene (HN) from both Najaf and Baghdad NDV isolates, specific primers for this gene were designed. After viral RNA purification, one - step reverse transcriptase - PCR was undertaken to amplify the NH gene and isolated it from the gel. Nucleotide sequence of the isolated NH gene from both virus isolates was determined. The purpose was to focus on molecular and phylogenetic analysis of the hemagglutinin - neuraminidase (HN) gene in both Iraqi isolates. The resulted nucleotide sequence of 647 nucleotides of the HN gene for both isolate were submitted to the GenBank database under the accession numbers KJ632972. To determine the origin of both Iraqi virulent isolates, HN gene sequence of both isolates was aligned with sequences of NDV isolates previously published in GenBank. The resulted alignment was then analyzed in terms of phylogenetic relation and variation. phylogenetic analysis of the isolated virus showed that both Iraqi isolates share significant similarity with 14 other international stains. Two strains of India origin and 12 was China isolates. The identity percentage was 99% - 100% with clustered group. Interestingly, Iraqi isolate was different from the neighbor countries such as Iran, and other Middle East countries. These results may indicate that certain migratory birds might have contributed to the distribution of NDV in Iraq. However imported infected beards from south East Asia may contribute to the current NDV outbreaks caused by foreign strain.

دراسة جزيئية مصلية وكيميائية عن الاصابة بطفيلي انابلازما مارجينالي في الابقار في محافظة الكوت == Some Molecular ,Sero - Prevalence And Biochemical Study of Anaplasma Marginale In Cattle In Al - Kut Province

اسم المؤلف: علا عبد الحسين عكار الكفاري
اسم المشرف: غيداء عباس جاسم الجبوري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: صممت الدراسة للبحث في انتشار مرض الانابلازموسسس بين قطعان الابقار في مناطق مختلفة من محافظة واسط وقد اجري البحث على 184 عينة دم جمعت من ابقارمشكوك في اصابتها تعاني من الحمى (°41c) , فقر دم شديد , شحوب الاغشية المخاطية ,هزال شديد وانخفاض في انتاج الحليب. و| The study was designed to investigate the prevalence of bovine anaplasmosis among cattle from various area in Wassit governorate , the investigation is performed on 184 blood samples collected from suspected cattle suffering from fever (41°C), severe anemia ,pale mucus membrane , progressive emaciation and drop in milk yield , includes (85) males and (99) females , aged < 1year to 2 - 3 years, during October 2012 to April 2013 from AL - Kut , AL - hayy , AL - Bashair , AL - Moufaqia and AL - Noamania areas to investigate antibodies against Anaplasma parasite by using indirect ELISA test and to determine the species Anaplasma marginale by using RFLP - PCR technique, also measuring some biochemical parameters to indicate the effect of the disease on liver function. Results of ELISA test showed that the rate of infection was 13.04 % , and there were different between age groups without significant difference were give 8 % , 11.25 % and 16.45 % in ages < 1year , 1 - 2 year and 2 - 3 years respectively. There was no significant differencerecorded between females that give study revealed that females were give 14.14 % rate of infection and males with 11.7 % rate of infection.The highest rate of infection was recorded in AL - Kut 17.14 % followed by AL - hayy and AL - Bashair were give 14 % ,10 % respectively and the lowest rate was recorded in AL - Noamania and AL - Moufaqia 8.33 % , 5% respectively, the study showed significant differences in incidence of disease between study districts and area in Wassit province under p > 0.05. The most sensitive method used for the diagnosis of anaplasmosis was polymerase chain reaction , DNA extraction was performed only on 24 blood samples which were positive for Anaplasma parasite by ELISA test, the extracted DNA from blood cells were analyzed by PCR and PCR - RFLP technique using primers derived from 16S rRNA gene and restriction endonuclease Bst1107I enzyme which recognized the sequence (GTATAC) in corresponding PCR product of A. marginale and cut it in the position 68, whereas the used restriction enzyme can not cut the corresponding PCR product of other Aanaplasma spp. and the result was 20 from 24 was positive for Aanaplasma spp. by PCR and 18 from 20 was positive for A. marginale. The results of liver enzymes activity showed significant increase in serum AST, ALT, CK and TBIL level in infected cattle (96.8±0.97), (42±0.52) , (406±2.06) and (0.95±0.24) respectively as compared with control (65.5±1.26 ), (21.4±0.45 ) ,( 142±14.17 ) and (0.27±0.05 ) respectively.

دراسة تاثير مستخلص الشاي الاخضر مقارنة بالانسولين على بعض الصفات الفسلجية والتعبير الجيني في الارانب المستحدثة تجريبيا بالسكري == Study The Effect of Green Tea Extract As Compared With Insulin On Some Physiological Parameters And Gene Expression In Experimentally Induced Diabetic Rabbits

اسم المؤلف: ماهر مهدي صالح العارضي
اسم المشرف: حيدر عبد الكاظم نغيش الزاملي
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسه الحاليه لتحديد التاثيرات المحتملة للمستخلص المائي للشاي الاخضر200 ملغم / كغم في اناث الارانب المستحدث فيها السكري تجريبيا. استخدام 35 انثى ارنب بالغة من النوع المحلي تراوحت اعمارها 6 - 10 اشهر معدل اوزانها 1 - 1،5 كغم. قسمت الى خمسة مجاميع مت | The present study aimed to determine the effect of aqueous extract of green tea (200 mg / kg B.W) in experimentally induced diabetic female rabbits.Thirty five female rabbits with arange age of 6 - 10 months and 1 - 1.5 kg weight were used.They are divided into five equal groups.The first group used as negative control (C),The diabetes melltitus (DM) was induced in T1,T2 and T3 by injection of single dose of alloxan 100 mg /kg B.W in marginal ear vein , The T1 assigned as positive control. The T2 treated with 200 mg / kg B.W of aqueous extract of green tea for 8 weeks.The T3 treated with 3 IU/ SC of insulin for 8 weeks while the T4 was given 200 mg / kg B.W of aqueous extract of green tea alone for 8 weeks. After 5 days of diabetes mellitus induction the blood samples were taken from the same vein, the rabbits which have blood glucose more than 200 mg / dl blood consider as diabetic. At the end of the experiment blood samples were taken by cardiac puncture and the blood serum was separated by Centrifuge to measure glucose , albumin, total protein ,liver enzymes and lipid profile and histological sections for pancrease,liver and kidney were done. the Real - time PCR was done to measure the gene expression of (Ins 1) and (Ins 2) which are responsible for insulin hormone production.the results were revealed that there is significant ( p <0.05) decrease in glucose,Low density lipoprotein,very Low density lipoprotein , Triglyceride , Total cholesterol , Alkaline phosphatase , Aspartate Transaminase and Alanine transaminase and significant increase (p <0.05) in Highy density lipoprotein , albumin and total protein in T2 group compared with T1, there is non significant differences in these parameters with T3 group , also there is significant decrease in ( p < 0.05) in Low density lipoprotein,Very Low density lipoprotein , Triglycerid , Total cholesterol and significant increase ( p < 0.05) in Highy density lipoprotein in T4 group compared with all groups. The gene expression results revealed that there is significant ( p < 0.05) increase in Ins 1 and Ins 2 gene expression in T2 compared with T1.The histological study of T1 was explain that there is severe congestion in pancrease with necrosis and absent of islets of langerhans while in the liver there is congestion with large thrombus in central vein with hepatic necrosis. In the kidney there is thrombus and congestion in renal tissue and destruction in renal tubules. While in T2 most damage caused by alloxan was disappeared through regeneration of cells of studied organs

دراسة تاثير الشاي الاخضر في وظيفة عضلة القلب للارانب المعاملة بالنترات == Study Of The Effect Of Green Tea On Cardiac Muscle Function In Nitrate Treated Rabbits

اسم المؤلف: حيدر غازي عبد الشهيد الفتلاوي
اسم المشرف: حيدر عبد الكاظم نغيش الزاملي
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

تشخيصCampylobacter jejuni باستخدام تقنية الوقت الحقيقي لتفاعل سلسلة البلمرة في محافظة المثنى == Detection Of Campylobacter Jejuni By Real - Time PCR In Al - Muthanna Province

اسم المؤلف: منى توفيق عبد
اسم المشرف: هدى عبد الهادي علي النصراوي
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض المشتركة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

سرطان الرئة الغدي في الاغنامPulmonary Adenocarcinoma in sheep (jaagsiekte disease) == Pulmonary Adenocarcinomao In Sheep (Jaagsiekte Disease)

اسم المؤلف: زينب حسين جياد
اسم المشرف: قاسم حليم كشاش
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الطب الباطني والوقائي
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

استعراض التهاب الضرع تحت السريري في ابقار الحليب == Review Of Subclinical Mastitis In Dairy Cattle

اسم المؤلف: وجدان حنون عطية
اسم المشرف: اسعد جاسم عبد
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الطب الباطني والوقائي
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى: