دراسة تاثير مستخلص الشاي الاخضر مقارنة بالانسولين على بعض الصفات الفسلجية والتعبير الجيني في الارانب المستحدثة تجريبيا بالسكري == Study The Effect of Green Tea Extract As Compared With Insulin On Some Physiological Parameters And Gene Expression In Experimentally Induced Diabetic Rabbits
Author name:
ماهر مهدي صالح العارضي
Supervisor name:
حيدر عبد الكاظم نغيش الزاملي
General topic:
Veterinary Medicine
Specific topic:
Physiology
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
English
University location:
Qadisiyah
First pages:
21T491 - p.pdf
Abstract:
هدفت الدراسه الحاليه لتحديد التاثيرات المحتملة للمستخلص المائي للشاي الاخضر200 ملغم / كغم في اناث الارانب المستحدث فيها السكري تجريبيا. استخدام 35 انثى ارنب بالغة من النوع المحلي تراوحت اعمارها 6 - 10 اشهر معدل اوزانها 1 - 1،5 كغم. قسمت الى خمسة مجاميع مت | The present study aimed to determine the effect of aqueous extract of green tea (200 mg / kg B.W) in experimentally induced diabetic female rabbits.Thirty five female rabbits with arange age of 6 - 10 months and 1 - 1.5 kg weight were used.They are divided into five equal groups.The first group used as negative control (C),The diabetes melltitus (DM) was induced in T1,T2 and T3 by injection of single dose of alloxan 100 mg /kg B.W in marginal ear vein , The T1 assigned as positive control. The T2 treated with 200 mg / kg B.W of aqueous extract of green tea for 8 weeks.The T3 treated with 3 IU/ SC of insulin for 8 weeks while the T4 was given 200 mg / kg B.W of aqueous extract of green tea alone for 8 weeks. After 5 days of diabetes mellitus induction the blood samples were taken from the same vein, the rabbits which have blood glucose more than 200 mg / dl blood consider as diabetic. At the end of the experiment blood samples were taken by cardiac puncture and the blood serum was separated by Centrifuge to measure glucose , albumin, total protein ,liver enzymes and lipid profile and histological sections for pancrease,liver and kidney were done. the Real - time PCR was done to measure the gene expression of (Ins 1) and (Ins 2) which are responsible for insulin hormone production.the results were revealed that there is significant ( p <0.05) decrease in glucose,Low density lipoprotein,very Low density lipoprotein , Triglyceride , Total cholesterol , Alkaline phosphatase , Aspartate Transaminase and Alanine transaminase and significant increase (p <0.05) in Highy density lipoprotein , albumin and total protein in T2 group compared with T1, there is non significant differences in these parameters with T3 group , also there is significant decrease in ( p < 0.05) in Low density lipoprotein,Very Low density lipoprotein , Triglycerid , Total cholesterol and significant increase ( p < 0.05) in Highy density lipoprotein in T4 group compared with all groups. The gene expression results revealed that there is significant ( p < 0.05) increase in Ins 1 and Ins 2 gene expression in T2 compared with T1.The histological study of T1 was explain that there is severe congestion in pancrease with necrosis and absent of islets of langerhans while in the liver there is congestion with large thrombus in central vein with hepatic necrosis. In the kidney there is thrombus and congestion in renal tissue and destruction in renal tubules. While in T2 most damage caused by alloxan was disappeared through regeneration of cells of studied organs
