دراسة جزيئية مصلية وكيميائية عن الاصابة بطفيلي انابلازما مارجينالي في الابقار في محافظة الكوت == Some Molecular ,Sero - Prevalence And Biochemical Study of Anaplasma Marginale In Cattle In Al - Kut Province
Author name:
علا عبد الحسين عكار الكفاري
Supervisor name:
غيداء عباس جاسم الجبوري
General topic:
Veterinary Medicine
Specific topic:
Veterinary Medicine
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
English
University location:
Qadisiyah
First pages:
21T493 - p.pdf
Abstract:
صممت الدراسة للبحث في انتشار مرض الانابلازموسسس بين قطعان الابقار في مناطق مختلفة من محافظة واسط وقد اجري البحث على 184 عينة دم جمعت من ابقارمشكوك في اصابتها تعاني من الحمى (°41c) , فقر دم شديد , شحوب الاغشية المخاطية ,هزال شديد وانخفاض في انتاج الحليب. و| The study was designed to investigate the prevalence of bovine anaplasmosis among cattle from various area in Wassit governorate , the investigation is performed on 184 blood samples collected from suspected cattle suffering from fever (41°C), severe anemia ,pale mucus membrane , progressive emaciation and drop in milk yield , includes (85) males and (99) females , aged < 1year to 2 - 3 years, during October 2012 to April 2013 from AL - Kut , AL - hayy , AL - Bashair , AL - Moufaqia and AL - Noamania areas to investigate antibodies against Anaplasma parasite by using indirect ELISA test and to determine the species Anaplasma marginale by using RFLP - PCR technique, also measuring some biochemical parameters to indicate the effect of the disease on liver function. Results of ELISA test showed that the rate of infection was 13.04 % , and there were different between age groups without significant difference were give 8 % , 11.25 % and 16.45 % in ages < 1year , 1 - 2 year and 2 - 3 years respectively. There was no significant differencerecorded between females that give study revealed that females were give 14.14 % rate of infection and males with 11.7 % rate of infection.The highest rate of infection was recorded in AL - Kut 17.14 % followed by AL - hayy and AL - Bashair were give 14 % ,10 % respectively and the lowest rate was recorded in AL - Noamania and AL - Moufaqia 8.33 % , 5% respectively, the study showed significant differences in incidence of disease between study districts and area in Wassit province under p > 0.05. The most sensitive method used for the diagnosis of anaplasmosis was polymerase chain reaction , DNA extraction was performed only on 24 blood samples which were positive for Anaplasma parasite by ELISA test, the extracted DNA from blood cells were analyzed by PCR and PCR - RFLP technique using primers derived from 16S rRNA gene and restriction endonuclease Bst1107I enzyme which recognized the sequence (GTATAC) in corresponding PCR product of A. marginale and cut it in the position 68, whereas the used restriction enzyme can not cut the corresponding PCR product of other Aanaplasma spp. and the result was 20 from 24 was positive for Aanaplasma spp. by PCR and 18 from 20 was positive for A. marginale. The results of liver enzymes activity showed significant increase in serum AST, ALT, CK and TBIL level in infected cattle (96.8±0.97), (42±0.52) , (406±2.06) and (0.95±0.24) respectively as compared with control (65.5±1.26 ), (21.4±0.45 ) ,( 142±14.17 ) and (0.27±0.05 ) respectively.
