المستخلص: The present study was carried out in Thi - Qar province during the period from beginning of January until the end of December 2015 to investigate the prevalence of infection with hydatidosis in human and other intermediate hosts. The molecular techniques was used to detect the strain of Echinococcus granulosus. The present study diagnosed 58 infection with hydatidosis in different organs such as liver, lungs, kidney and spleen of surgically treated patients in AL - Hussein Imam Teaching Hospital inNassiriyah city, centre of Thi - Qar province. The total number of hydatid cysts was 72 cyst in different organs with total intensity of infection 1.24. The females recorded highest percentage of infection 67.24% than males 32.76 %, and the age group 21 - 30 year showed highest percentage of infection 24.14 % . The lowest percentage recorded in age group more than 60 year 5.17 %.The results of study showed that right lobe of liver was more affected 58.62 % than left lobe 13.79 %. The percentage of left lung infection was 10.34 % while right lung 8.62 %. The total average of fertile cysts in infected organs was 81.82 % and sterile cysts 18.18 %. The highest percentage of infection was recorded in house women 87.18 %. The number of recorded cases in Nassiriyah district was 21 case with percentage of infection 36.21 %.During the same period a total of 1303, 1287, 405, 90, and 294 head from sheep, cattle, buffaloes, camels and goats were examined respectively.The percentage of infection was 7.83 %, 7.69 %, 3.46 % and 2.22 % in sheep, cattle, buffaloes and camels respectively, and no infection was recorded in goats. The intensity of infection was 4.07, 3.27, 1.64 and 2 in sheep, cattle, buffaloes and camels respectively. The results of statistical analysis showed no significant differences (P????0.05) in percentage and intensity of infection.The percentage infection of liver, lungs and both together in sheep was 5.45 %, 1.23 % and 1.15 % respectively, and there was no significant differences in percentage of infection but intensity of infection differed significantly(P????0.05). The percentage of liver, lungs, both together and spleen infection in cattle were 3.11 %, 2.95 % , 1.55 % and 0.08 % respectively while intensity of infection in liver, lungs, both together and spleen was 2.5, 2.18, 7 and 1 respectively. The percentage of liver, lungs and both together infection in buffaloes were 2.22 %, 0.99 % and 0.25 % respectively while intensity of infection in liver, lungs and both together was 1.89, 1 and 2 respectively. No significant differences in percentage and intensity of infection in camels andboth percentage and intensity of infection in liver and lungs were 1.11% and 2 respectively.High significant differences was recorded between percentage infection in males and females of animals. The percentage of infection in females were 15.87 %, 13.42 %, 6.99 % and 3.45 % in sheep, cattle, buffaloes and camels respectively. Highest percentage of infection were 0.72 %, 0.18 % and 0.46 % in males of sheep, cattle and buffaloes respectively. No infection were recorded in males of camels.T he age was effect on percentage of infection and big aging groups recorded in sheep, cattle, buffaloes and camels. The present study recorded significant differences in percentage of fertile hydatid cysts in liver and lungs of animals and highest of percentage of fertility recorded in liver and lungs of sheep 84.21 % and 79.24 % respectively and lower percentage of fertility was in liver of buffaloes 11.11% and liver of cattle 14.70 %. The lower percentage of sterile hydatid cysts was in liver and lungs of sheep 15.79 % and 20.75 % respectively and highest percentage recorded in liver of buffaloes 88.88 % and spleen of cattle 100 %.S t a tistical analysis using of ANOVAs test showed no significant differences in total length, length of blade and width for large and small protoscolices hooks of hydatid cysts isolated from liver and lungs of sheep, human, cattle buffaloes, camels and hooks of sheep which is showed genetic variations (new strain).The PCR - RFLP technique was used in identification of E.granulosus strains infecting human and intermediate hosts in Thi - Qar province. The molecular study is carried out on 75 hydatid cysts collected from human, sheep, cattle, buffaloes and camels. The DNA is extracted from protoscolices of hydatid cysts isolated from infected organs. PCR - RFLP technique showed that sheep strain G1 of E.granulosus is predominant in Thi - Qar province depending on amplification of rDNA - ITS1 gene. The PCR products in all DNA samples showed 1000 bp approximately. Alu1 endonuclease with rDNA - ITS1 demonstrated two bands 800 bp and 200 bp approximately, while Rsa1 endonuclease showed two bands 655 bp and 345 bp approximately which is similar to sheep strain genotype 1.Sequence analysis for rDNA - ITS1 gene showed the presence of sheepstrain genotype 1 which is similar 100 % to sheep strain in gene bank exceptone isolate (sheep liver) was differs from other strains in position ofnucleotide sequence. It was similar 99 % for sequence of sheep strain. Thedata base of this new strain was recorded in gene bank for DNA sequence National Center for Biotechnology Information (NCBI) and European Nucleotide Archive (ENA) for the first time globally under the name Echinococcus granulosus strain WH1AW2 (GenBank : LT547814). Polymerase chain reaction technique and sequence analysis of mtCOX1 gene showed the presence of sheep strain G1 of human, sheep, cattle, buffaloes and camels. The sequence analysis showed the genetic variation in mtCOX1 gene for hydatid cyst in infected sheep represented by point mutation type transition which caused change Adenine to Guanine, and sequence data base of the sample recorded for first time in NCBI and ENA (GenBank : LC203589). Buffaloes strain G3 isolated from human lung recorded for first time in Thi - Qar province depending on comparison of samples sequences with source strains recorded in gene bank (NCBI) using "BLAST". The size of bands for mtCOX1 gene was 450 bp in all samples

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

السنة: 2016

الموضوع العام: علوم الحياة

اسم المؤلف: وسام جاسم حنش

استخلاص وتنقية وتشخيص ببتيد كلايكوسيدي من الحبار Loligo sp. ودراسة تاصيرة على خط الخلايا السرطانية Hela Cell Line والطبيعية REF Extraction , Purification, Characterization Glycosidic Peptide From Loligo sp. and Study Effect On Cancer Cell Line (Hela) and Normal (REF)

المستخلص: The study was designated to investigate the capcity of pure Loligo sp extract on two cell lines : - the cancer cell line Human cervix uteri epitheloid carcinoma (Hela) and Rat Embryo Fibroblast cell line (REF) as normal cell line.The study included extraction of tissue (whole body) of Loligo sp by using 30% ammonium sulfate. and chemical group detected in the extract by using qualitative chemical test and the result showed that the crud and pure extract contained alkloides, proteins, carbohdrates, saponines, flavonoids, aldehyde &keton group and phenol group .and the extacte does not contain glycosides.The crude extraction was purified by column chromatography using sephadex G - 25. and the purity of extract detected by using polyacryl amide gel electrophoresis under non denaturated condition. The result showed that the extracted riched with amino acid and some carbohydrate by using high performance liquid chromatography (HPLC) with compared standerd amino acid and suger.Infrared Spectroscopy of purified extract shows that it contain (N - H - C - OH) to emphasize on found amino acid in the extract.The cytotoxicity effect on cell lines study by four concentraction ( 125, 250 ,500 and 1000)ϻg/ml were prepared and tested on cell line with five replicates for each concentraction, the optical density of cell growth read by the Elisa reader 550 nm and use by tetra zolium bromide (MTT). The result for in vitro study showed that all concentraction had high inhibition on tumor cell line and a highly significant inhibition in (Hela) line was recoreded 42.79% at exposure time 48hr in the concentraction 1000 ϻg/ml the effect was dose dependent. The inhibitory effect of extract on Hela cell line higher than in the REF. while it was less effect of extract on Rat Embryo Fibroblast cell line after exposure time 48hr.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم - قسم علوم الحياة

الدرجة: ماجستير

الموضوع الدقيق: الاحياء المجهرية

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: بلسم انيس مارينا ضمياء قاسم سكر

اسم المؤلف: هيفاء عدنان منصور الموسوي

مستويات الاديبونكتين والابلين والهرمونات الاخرى والمعايير الدموية اثناء الحمل وفي مرضى متلازمة المبيض متعدد الاكياس Adiponectin, Apelin and other Hormones Levels and Blood Indices during Pregnancy and in Patients with Polycystic Ovaries Syndrome

المستخلص: The present study aimed to determine the levels of adiponectin and apelin and their relations with all parameters. Investigation in the present study included; hormones, lipid profile, liver enzymes activity and haematological profile in groups of Iraqi Basrah pregnant women during different months of pregnancy and to correlate these levels to maternal age, Body Mass Index, excepted parity and sex of fetuses and correlated with level of adiponectin and apelin hormones only and the results were compared with non - pregnant females as control group. The present study included (180) women ranging from 20 - 40 years old, out of them (70) pregnant and (20) non - pregnant women with polycystic ovary syndrome (PCOs), and (90) females non - pregnant were considered as control group. The pregnant women attended the obsteric units in the general state hospitals in Basra Governorate. They are as follows : Al - Basra General Hospital for Maternity and Childhood, Al - Mawane, Al - Fayha’a and Basra General Hospital which drain most patients from urban and rural areas. None of the selected women suffered from any type of disease. Required data were collected by the researcher depending on direct interview with women before being admitted to the study, using a questionnaire including information on age, parity, month of pregnancy, and geographical area..The Women were divided into two age group (≤ 27 and ≥28 years). Moreover, females were also divided according to BMI into normal (≤ 25), over weight (25 - 30) and Obese (≥30).The month of gestation was determined not only from the date of the women’s last menstruation (LMP) but also according to the ultrasonic reports that pregnant women had. Blood samples (10 ml) were taken from pregnant women at the end of each month of pregnancy and from control group as well as from women with PCOs. Each blood sample was divided into EDTA tube (2 ml) which was used for complete hematological picture study (RBC, Hb, PCV, PLT, WBCs count and WBCs differential count) and the plain tube (8 ml) where the serum was obtained, for biochemical study (Adiponectin, Apelin, testosterone, Progesterone, estradiol, follicular stimulating hormone, luteinizing hormone, insulin, lipid profile and liver enzymes).The results indicated that maternal age had no significant effect on the level of adiponectin, apelin, FSH and LH hormones, while the study showed a significantly effect on level of progesterone, estradiol, testosterone and insulin hormones between pregnant and non - pregnant women. Adiponectin levels decreased in pregnant and non - pregnant females with increased body mass index whereas apelin, estradiol, progesterone and insulin levels increased. When the comparison between pregnant and non - pregnant women was done, a decrease in adiponectin and apelin was noticed. Accompanied by an increase in the level of estradiol, testosterone, progesterone and insulin hormones in pregnant women compared to non - pregnant women.Women with PCOs showed low levels of both Adiponectin and apelin with high levels of Testosterone and Insulin.Results also revealed that age had no effect on total cholesterol, triglycerides, and HDL, in both pregnant and non - pregnant females, but when the BMI was taken in consideration, high levels of these parameters were found in - group of BMI (obese). In addition, the present study showed that age had no effect on liver enzymes (AST and ALT) while ALP showed that the behavior increased up as a different high body mass index in pregnant women, in comparison with the control group. However, ALP activity also increased in high BMI group.Hematological study showed that age had no effect on RBC, Platelets, and hematocrit. Similar results were found when BMI was taken in consideration. Total WBCs showed a non - significant increase in pregnant women as compared with the control group. Adiponectin levels were significantly higher in pregnant females carrying female fetuses compared to those with male fetuses, while the other hormone apelin did not differ between the two groups. The Result of the present study revealed that adiponectin was significantly decreased as apelin was significantly increased with the increased parity.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم - قسم علوم الحياة

الدرجة: دكتوراه

الموضوع الدقيق: الحيوان - الفسلجة

السنة: 2015

الموضوع العام: علوم الحياة

اسم المشرف: طه جاسم الطه علي فالح الاسدي

اسم المؤلف: هناء سلمان كاظم

دراسة مسحية للبكتريا المرتبطة بالتهاب الزائدة الدودية وتحديد الحالة النسجية للمصابين في محافظة البصرة Survey of bacteria associated with appendicitis and determination of Histological patient state in the province of Basrah

المستخلص: The current study has been done to determined pathogenic bacteria that associated with appendicitis .This study includes ninety samples of removal appendix taken form patients who cleared diagnosed as appendicitis infection by specialised doctors in general Basrah hospital and Al - Sadir teaching hospital for the period between September - 2013 and June - 2014.Distribution of appendicitis appear in all age groups, 10 - 20 year and 20 - 30 year were the most infected, as well as the infection appear in both gender, but rate of infection in male was relatively higher than female 68(59%), 47(41%) respectively. The ratio of the city's population has overcome the rural population 96(83.5%),19 (16.5%) respectively. The percentage of samples that gave positive culture was 80( 88.9%), while 10 (11.1%) of these samples negative culture. The study reveals 15 different bacterial isolation, the most common bacteria was Escherichia coli 80(44.9%) while other species was appears in less percentage Shigella dysenteria 14(7.9%), Salmonella enterica typhi 10( 6.5 %), Pseudomonas aeruginosa 8( 5.6 %) , Klebseilla Pneumonia 6(4.3%) , Klebseilla oxytoca 3(1.7%), Morganella morganai 7 ( 9.3 %) Neisseria spp. 6 ( 9.5%) , Enterobacter cloaca 4 ( 2.2 %) ,Serreatia spp. 3 )7.1 %(, Citrobacter ferundii 2 (7.7%) Proteus vulgaris 7) 6.5 %( Staphylococcus spp. 14(1.3%), Streptococcus spp. 72(5.1 %) and Bacillus subtilis 8)5.6 %(. Laboratory diagnosis for blood samples included estimated of total WBCs and found that (31%) of patients have natural WBCs values while the other patients have high values.Antibiotic sensitivity test to E.coli isolates towards 20 antibiotics because she is the most common bacteria showed that all isolates were resisted for most antibiotics which used in test especially for β - lactam group, and the isolates of E.coli were multi resistance for antibiotics.Identified minimum inhibitory concentrations MIC for some antibiotics The results showed that E.coli bacterial isolates's 30 isolation have shown resistance to anti AMO as MIC values ranged from 128 - 1024 Mcgm / ml while the MIC for Anti AMP values between 4 - 128 Mcgm / ml, As for antibiotics and Cefixim , Ceftriaxone, Cefotaxime, and it was her MIC values ranging between (1 - 128) Mcgm / ml Plasmid profile of E.coli isolates investigated to study the correlation between plasmid profile and antibiotic resisitant marker and results from agarose gel electrophoresis revealed that all E.coli isolates contain one plasmid band.This study includes the detection of some genes that encode to beta - lactamase enzymes in E.coli which were responsible about multi antibiotic resistanc and these genes loaded on plasmid DNA for ten isolaes and found that 5(50%) from isolates have blaTEM gene and 5 )40%( have blaCTX gene and 1(10%) have blaSHV gene. This study also considered note the general appearance of appendix samples , some of them are enlarged and surrounded by vesicles, some with fibrous walls and ulcerated with mixed colors, then examined the histological changes it, the study showed changes in histological structure of the excess was extensively congestion of blood vessels, veins in serosa and subserosal layers and increased the amount of diffuse lymphoid tissue in the layers of the appendix walls.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم - قسم علوم الحياة

الدرجة: ماجستير

الموضوع الدقيق: الاحياء المجهرية - البكتريا

السنة: 2015

الموضوع العام: علوم الحياة

اسم المشرف: محمد عبد الامام احمد نوري حنون جاسم

اسم المؤلف: هدى عبد الرحيم مذكور

دراسة فعالية بعض مستخلصات الطحلب الاخضر Enteromorpha intestinalis (L.,1820 على الاكياس العذرية للمشوكة الحبيبية Echinococcus granulosus (Batsch,1786 وكمضادات لخط الخلايا السرطانية نوع HeLa Study of the activity of some of the green alga Enteromorpha intestinalis (L.,1820) extracts on Hydatid cysts of Echinococcus granulosus (Batsch,1786) and as anticancer cells type HeLa

المستخلص: The present study was conducted in order to evaluate the activity of green alga Enteromorpha intestinalis extracts (methanol and hexane extracts) on growth and development of hydatid cysts of Echinococcus granulosus in vitro and in vivo by using mice type Mus musculus Balb\c that in comparing with albendazole drug. Anti - cancer activity was also evaluated in vitro for both algal extracts by using HeLa cells. Chemical compositions of algal extracts were analyzed by using Gas chromatography - mass spectroscopy (Gc - mass)technique . The hydatid cysts were collected from livers and lungs of infected sheep which slaughtered in Basrah abattoir. While, The algae specimens were collected from Abu Sokhir marshland area /north of Basra. The results revealed that E.intestinalis chemical compounds are carbohydrate , alkaloids, phenols , saponins , triterpenes and sterols compounds. In vitro study showed that the 1 mg / mouse weight of albendazole drug was able to reduce the percentage of protoscolices activity to zero after the third day of treatment. While, the dose 6 98 mg / kg of methanol extract inhibited all protoscolices after five days of treatment, followed by the dose 804 mg / kg of hexan extract that inhibited all protoscolices after six days of treatment.In vivo study revealed that the methanol extract (698 mg / kg) treatment was the best in reducing the weights of organs significantly in comparison with the positive control group it was 2.192 , 0.192 , 0.434 and 0.282 g of liver, spleen, lung and kidney respectively. The methanol extract (698 mg / kg) treatment lowered the average number of hydatid cysts (2.6) more than other extracts treatments , then followed by hexane extract (804 mg / kg) treatment it was 2.8 cyst .This study has identified that the liver enzymes Aspartate transaminase (ALT) and Alanine transaminase (AST) values in the serum of positive control group were higher significantly than negative control group the value of the enzyme ALT was 79 IU / l and the value of the enzyme AST was 118.4 IU / l.All methanolic extracts groups showed a preference in enzyme's values compared with other treatments groups , the value of ALT enzyme of 698 , 688 and 678 mg / kg of methanol extract groups were 46.8 , 45.8 and 44.6 IU / l respectively, while all hexane extracts groups showed a preference in AST enzyme values compared to the other treatments groups, the value of AST enzyme of 804 , 794 and 784 mg / kg of hexane extract groups were 73.6 , 70.2 and 70.4 IU / l respectively. Found a significant increase in the number of white blood cells WBCs in a positive control group ( 4.6 × 013μ), compared with a negative control group and the other groups, While it has been found significant decrease in the Hb (5..8deciliter) and Packed cell volume (PCV) (31..%) values in positive control group as compared with control negative group. The study found that the number of white blood cells WBCs n the methanolic extract group (678 mg / kg) was 4.06 × 103 microliter was as same as in the negative control group, In contrast, hexane extract group (804 mg/ kg) has did not significant difference with negative control group in the Hb and PCV were 11.5 g / dl and 36.8% respectively .Histological study confirmed that there are the histological changes in positive control group included congestion , bleeding , degeneration , infiltration of inflammatory cells and hyperplasia. Whereas the histological changes in group treated with albendazole were bleeding, infiltration of inflammatory cells , atrophy of the glomerulus and fibrosis in some areas . In algal extracts groups the histological changes were infiltration of inflammatory cells, bleeding and congestion.The Gc mass results revealed that the ethanol extract contains loliolide , ethyl stearate , palmitic Acid , ethyl palmitate , phytol , ethyl oleate , ethylhexyladipate and squalene compounds. While, hexane extract has N, Ndimethyltetradecylamine , diisobutyl phthalate , palmitic Acid , ethyl palmitate and ethylhexyladipate compounds.Anti - cancer study showed the algal extracts have an inhibition activity against cancer cells, The half maximal inhibitory concentration (IC50) value of the methanol extract was 79.08 μ/ ml, While, the IC50 for hexane extract was 156.3 μ/ ml.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: التقنيات الاحيائية

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: صبيح هليل المياح احمد محسن عذبي

اسم المؤلف: غزوان طالب نوري الجابر

دراسة تشريحية تصنيفية لبعض من نباتات العائلة السوسبية Euphorbiaceae في العراق Anatomical and Taxomanical study for some plant of Euphorbiaceae family in IRAQ

المستخلص: The study was conducted to investigate several taxonomic aspects which comprised the micro - morphological studies of epidermal layer of leaves, pollen grains, fruits and seeds.In addition to that, the anatomical and molecular studies for 12 species of Euphorbiaceae family which belonged to five genera AndrachneL. , Chrozophora L., Euphorbia L, Mercurialis L. and RicinusL., were performed. One representative species for each genuswas selected; in exception 8 species were chosen for the genus Euphorbia..The current results confirmed that the anatomical characters showed valuable taxonomic traits which can evidently be used to distinguish between species. The most prominent trait was mesophylla tissue in leaves which were observed in three categories;1 - Bifacial leaves : - which found in C.tinctoria, Euphorbia sp ., E. milii, E.annua and R.comminus 2 - Uni - facial (heterogenus) leaves : - this category of the mesophyll was detected in two species only A.telephioides and E.prostrata3 - Uni - facial (homogenus) leaves : - this category were noticed in five species; E.denticulata ,E.helioscopia ,E,macrocarpa , E.microsphaeia and E.peplus The second important comparative anatomical character was the Mid rib, which are in four types with several shapes from which discrimination between the species can be made : - 1. Convex - concave mid rib : - that was showed in E.helioscopia, E.macrocarpa, E.milii and M.annua.2. Convex - convex : - This type was found in three species C.tinctoria , Euphorbia sp.and R. comminus3. Flat - Convex : - This type of mid rib was observed in A.telephioides E.microsphaera, E.peplus and E.prostrata4. Flat - Flat : - This type was observed in only one species E.denticulata In stem, the most prominent parts of taxonomic evidence were pith and vascular bundles.In according to the pith, species can be divided into hollow stem (without pith) and solid stem (containing medulla good configuration). Hollow stem was clear in the stems of A.telephioides , E.microsphaera , E.prostrata and M.annua. While, the solid stem wasseen in the remaining species. Vascular bundles were existed in two types; that's connected vascular bundles that's characterized the hollow stems, and separate vascular bundles. In most of the species with the exception of M. annua, stem with scatteredvascular bundles were in cortex As well as, petiole which have features that a clear taxonomy values .either by outline of petiole or its vascular arc.Micro - morphological studies of epidermal layer of leaves showed very important taxonomical value. This layer of leaves reveals variation in shape of ordinary epidermal cell and nature of anticline cell wall, stomata complexes and Epicuticlular wax crystals.Micro - morphological of pollen was taxonomic evidence as well. It can be considered to divide the species of Euphorbiaceae family into two groups based on the number of germination apertures 1 - Hexocorporate : - this type was confined to C.tinctoria2 - Tricolporate all species studied remainingThe sculpture of pollen wall provided beneficial information invested in the species identification.Both seeds and fruits showed a distinctive role in species identification. Micromorophological study of seeds for species which belong to six genera Andrachne , Chrozophora, Euphorbia, Mercurialis , Phyllanthus and Ricinus were achieved by using Scanning Electron Microscope(SEM). The study concentrated on micro - morphological aspect of Caruncle, seed surface ornamentation and the shape of epidermial cell wall. The result revealed that most studied species were with carunclated seeds ,but some specieswere with ecarunclated seeds as in A.telephioides L. ,C. tinctoria( L) Raf , E.prostrata Atin and P. maderaspatensis L Seed surface ornamentation characteristics showed a significant taxonomical value.Furthermore, current result showed that there are a highly variation in shape, size and color of the seeds walls. With respect to the fruits characteristics, which showed variations in number of mercarpi, shape, size, color and surface ornamentation that can be adopted in the separation of the species studied Molecular study provided significant support for phenotypic and anatomical classification and support genetic convergence between the species in Euphorbiacea family. The genus Euphorbia species showed a significant genetically convergence and participated in predecessors monophyletic. However, the genera in Euphorbiacea family under study probably originated and evolved from different ancestors’ polyphyletic.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: النبات

السنة: 2016

الموضوع العام: علوم الحياة

اسم المؤلف: امل علي ياسين الحسن

دراسة تصنيفية وحياتية للديدان الخيطية عائلة Tetrameridae Travassos, 1914 المتطفلة على بعض انواع الطيور في هور السناف محافظة ذي قار Taxonomic and Biological Studies of Nematodes of the Family Tetrameridae Travassos, 1914 in the Some Birds in Sinaph marsh Thi - Qar province

المستخلص: A total of 401 represented by 128 birds of Himantopus himantopus which belong to the family Recurvirostridae , 93 birds of Chettusia leucura which belong to the family charadriidae and 180 birds of Sturnus vulgaris which belong to the family sturnidae at the period from April 2014 to march 2015 in Al - Sanaf marsh , Suq - Al - Shuyukh city southern of the Thi - Qar province,for searching the nematodes which belong to the family Tetrameridae. Four species of nematodes, which belong to the family Tetrameridae, were isolated in the current study. They were T.nouveli, T.dubia, T.spinosa and Microtetrameres inermis. The H. himantopus was infected with the nematode T.nouveli and the prevalence of infection was 25.8% , bird.Furthermore, the Chettusia leucura was infected, the mean intensity was 3.3 worm in each infected birds. The nematode T.dubia was isolated from H. himantopus with the isolated from the Ch. Leucura mean intensity was 1.7.The nematode T.spinosa was isolated from H. himantopus the the mean intensity of infection was 2.3 and it was isolated from the Ch. leucura , the prevalence of infection 4.3% and the mean intensity was 1.8 worm in each infected worm.The record of these three nematode is considering the first record in Iraq.Microtetrameres inermis was isolated from the Sturnus vulgaris, the each infected birds.Himantopus himantopus from April 2014 to March of 2015.This bird was infected with three species from family Tetrameridae, The seasonal variation study of infection prevalence of Tetrameres spp.in, H. himantopus that there was II Summarycomplete seasonal cycle for nematodes T.nouveli, T.dubia , and T.spinosa .The nematode T.nouveli had hightest infection prevalence in summer the decrease in autumn, winter and spring while the infection prevalence of nematode T.dubia was hight in summer and autumn then decrease in winter and spring. The infection prevalence of nematode T.spinosa was generally low with little increase in autumn .The results of the current of the Ch. leucura indicated that this bird was infected with three species which nematodes T.nouveli, T.dubia and T.spinosa and they had seasonal appearance . There was not any significant effect of host sex on prevalence of infection, and it showed decreased in prevalence of infection when the bird's weight was increased.The study of lifecycle of nematodes : T.nouveli ,T.dubia and M. inermis revealed obtainment the larval stages after success the infection experimental of the grasshopper Schistocerca gregaria for the first time the three isolated nematodes from family Tetrameridae in this study which it was T.nouveli , T. dubia and M.inermis and was included the lifecycle of the three species . The differences were including the period of time to produce and developing the larval stages in addition to the differences in their shapes, it was revealed that were clear differences in the structure and their shape of the tip the posterior end of the third larval stage. The end of the third larval stage of the nematode T. nouveli had three caudal papilla and the posterior end of nematode T.dubia had five caudal papilla while the end of the third larval stage of M.inermis did not have any caudal papilla , so we can distinguish these nematode larval stage depended on the prevalence this character in their third larval stage.The experimental infection of the Columba livia domestica with nematode M.inermis was successful.While the experimental infection to the Columba livia domestica with nematode T.nouveli ,T.dubia .was failed The experimental infection of the Gallus domestica with nematodes of Tetrameridae T.nouveli, T.dubia and M.inermis was failed too.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: باسم ھاشم عبد الله

اسم المؤلف: نهى جبار عبد الركابي

دراسة تشخيصية وحياتية لعلقة المياه العذبة Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea في اهوار السويب من محافظة البصرة جنوب العراق Identification and Biological study of the Freshwater Leech Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea) in AL - Swaib Marshes From Basrah province south of Iraq

المستخلص: The species Dina punctata is recorded for the first time in Iraq (from Al - Swaib marshes). The identification was conducted morphologicaly and molecularly characteristics. The morphological features summarized as : - - The species D. punctata has vermiform body compressed ventrally, ranging in length from 55 - 60 mm and in diameter from 0.5 - 2 mm. The individuals have a large ability for extening. The pointed anterior end carries small anterior sucker which resembles lips surrounding the mouth opining, while the rounded posterior end carries large rounded posterior sucker. - This species is characterized by heterogeneous features such as the color of the body which changes from blackish gray for Adults to dark brown for Juveniles, as well as the number and arrangement of eyes which is in general four pairs arranged as reverse U shape located on the dorsal anterior edge. The first two pairs of eyes are located on the middle line of the body while the third and the fourth pairs are each located on one side of the body behind the anterior pairs. - The body consists of 34 segments, each of them is divided to five secondary annuli. The fifth annuli of each segment is divided into two subsections, thus the main segment becomes consist of six annuli. - The male and female gonopores are open on the ventral surface of the 12th segment, separated from each other by two annuli, the male gonopore is usually larger and clearer and located forward in the middle of the groove which is separate the annuli b2 and a2 respectively. The female gonopore is smaller and located in the back of the male gonopore in the middle of the groove which is separate the b5 and b6 annuli respectively.Because of the instability in some morphological features, the species D.punctata also identified in the present study according to genetic features.During the genetic study, the region of internal transcription space (ITS2) in DNA was isolated from leech specimens by using special primers for region internal transcription space (ITS2), after isolation, the ITS2 genes are amplified.The results of polymerase chain reaction of DNA were obtained of single bands of 580 base pairs molecular weight for all degrees of hybridization. The sequence of each sample after detecting was entered in the database of National Center Biological Information (NCBI) and the result showed that the sequences of all samples are belonging to the species D. punctata. In the field study, monthly samples of leeches D. punctata was collected from the marshes of Al - Swaib on the eastern side of Shatt Al - Arab river inQurna northern Basrah Province during the period from January 2015 to December 2015. A total of 235 individuals and 623 Cocoon were collected during the study period. The highest number was 58 recorded for individuals during May 2015, while being 335 recorded for cocoons during February 2015. On the other hand there is no occurrence recorded during July - August for individuals, and June - November for cocoons.The study investigates the water temperature, salinity and abundance of individuals and cocoons. The statistical analysis showed negative correlation between temperature and abundance of both individuals and cocoons, while the correlation of salinity was slightly positive with abundance of individuals and cocoons.The study of population sizes structure showed that the size class 40 - 50 mm has the highest frequency during the study period compared with the other size classes. On the other hand, the highest percentage of frequency was 66.7%, which also recorded for the size class 40 - 50 mm.Laboratory experiments showed that the members of a species D.punctata able to tolerate saline concentrations between 2 - 6 psu the survival rate 100%, Also, these worms (D. punctata) feed by different ways, it can be considered Predatory Macrophagous and Scavengers, its food materials consist of invertebrate animals such as Oligochaetes, Lumbriculids, Chironomid larvae; fleshy body of some aquatic snails; and on dead fishes. In the laboratory, the leeches fed well on flesh and livers of chicken, sheeps and caws, The laboratory also studied, Three different types of food effect (chicken liver, zooplankton, and animal feed) on the growth of young leech for a period of six weeks.From the field and laboratory observations, the specie D. punctata reproduces sexually by cross fertilization. The male and female gonopores are easily observed in adults which ranging in length from 45 - 60 mm. as well as the sperm sacs on the mid ventral surface of the body during the reproduction season, which extend in our study during two periods : January - May and December 2015. The sexual reproduction processes were studded in detail and the embryonic development in cocoons was divided into five hypothetical stages according to morphological and anatomical changes and these stages are : - 1 - Depositing Cocoon Stage.2 - Primary Cleavage stage.3 - Development and extension.4 - Differentiation of regions stage.5 - Complement and release stage. The histological structure of the body wall, suckers, reproductive organs and digestive tract of adult leeches were investigated. The body wall consists of thick layers of circular, longitudinal and oblique muscles.The structures of the male reproductive system such as genital atrium, sperm sacs and testis were observed. There are two lateral blood sinuses around the mouth cavity in addition to many nerve cells. The posterior sucker is muscular, and the muscle cells are as a dense net in the peripheral part, while are as a loose cells in the middle what give it a sponge appearance, in addition to primitive glomerular cells, nerve cells and mucus cells

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: الحيوان - اللافقريات

السنة: 2016

الموضوع العام: علوم الحياة

اسم المؤلف: ازهر محمد غالي الخزعلي

معاملة بذور الذرة الصفراء Zea mays L. بحامض السالسياليك واثرها في تحمل الشد المائي المستحث بمادة الكلايكول متعدد الاثلين The treatment of Corn seeds (Zea mays L.) with salicylic acid and its effects on drought tolerance induced by polyethylene glycol (PEG)

المستخلص: The study was conducted in Plant physiology laboratory, department of biology, college of education for pure sciences, University of Basrah from 1/6/2015 till 10/7/2016 in order to evaluate the effect ofpretreatment (soaking) seeds of corn (Zea mays L.) cv. Baghdad 3with salicylic acid (SA) on germination, early seedlings growth and to minimize the effect of water stress induced with poly ethylene glycol (PEG). The study included the following experiments : 1 - Effect of SA and PEG on germination using a hydroponic culture and Hoagland solution in which corn seeds were soaked in three concentrations (0.1,0.3,0.5) mMof SA in addition to control treatment with distilled water for six hours. Water stress were induced with PEG at concentrations of 0 (distilled water),1%,2%,3%,6%,9% and 12%. This experiment indicated that the optimum soaking concentration with SA was 0.5 mM and that there was no germination at 12% PEG.2 - Effect of interaction between corn seeds pretreatment with DW, 0.5mM of SA for six hoursand PEG at all previous concentrations except 12%, on early corn seedlings growth using hydroponic culture and Hoagland nutrient solution, for three weeks.3 - Effect of interaction betweencorn seeds pretreatment with DW, 0.5 mM of SA for six hoursand water stress induced with PEG at 3% and 6% on vegetative growth using pots filled with a mixture of sand and peat moss for three months.The parameters were evaluated after each of the previous experiments, also , Biochemical test . Plant height, leaves number, root length and number, dry and fresh weight for shoot and roots, while the followingparameters were evaluated in corn leaves after pots experiment : 1 - Biochemical tests included : proline and glycine betain concentrations, total carbohydrates, total chlorophyll, sodium and potassium ions concentrations.2 - Leaves anatomical study to compare the effect of PEG inducedwater stress and the role of its interaction with pretreatment with SA on thegrowth and development of internal structure of corn leaves.The following results were estimated : 1 - Germination percentage : increasing water stress in the nutrient media reduced the % germination significantly when compared with control treatment. Seeds pretreatment with SA enhanced the germination percent and the highest percent was at interaction between PEG at 1%, 2% and SA at 0.5 mM.2 - Growth parameters for seedling at hydroponic culture indicated that plant height, fresh and dry weight for shoot and roots have increased significantly when seeds were pretreated with 0.5 Mm SA All theprevious parameters were reduced significantly under PEG induced waterstress especially at 9%. Interaction between SAand PEG caused apositive significant increases in all studied parameters especially atconcentrations less than 6% and 9% PEG.3 - Growth parameters for corn plants grown at pots have showed a significant increase when pretreated with SA compared with that treated with DW. PEG caused a significant decrease in the growth parameters IIIespecially at 6%. Interaction between DW and 6% PEG recorded the lowest growth in all studied parameters. 4 - Biochemical tests : There was a significant increase in total carbohydrates and total chlorophyll content in leaves of corn plants pretreated with SA in compare with that pretreatdwith DW while the content of proline and glycine betaine were higher significantly in leaves pretreated with DW.In case of PEG induced water stress, the highest total carbohydrates content was at 6%, highest chlorophyll content at 3% while proline and glycine betaine highest content were at 6% PEG. 5 - Sodium and Potassium Concentration : The highest potassium concentration was at plant treated with SA while the highest sodium concentration was at plant treated with DW. Increasing PEG concentrations caused a significant increase in potassium concentration and a significant decrease in sodium concentration. Interaction results revealed that the highest potassium and the lowest sodium concentrations were at interaction between SA and PEG at 6%. 6 - Anatomical study results revealed that increasing water stress effected the leaves tissues structures and this were cleared at vascular bundles tissues (xylem and phloem tissues) and bundle sheath. Upper and lower epidermis, polliform cells were also affected by increasing water stress.The anatomy of leaves also showed that there was a positive enhancement in the structure of leaves tissues in the plants grown from pretreated seeds with SA.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية

الدرجة: ماجستير

الموضوع الدقيق: علوم الحياة

السنة: 2017

الموضوع العام: علوم الحياة

اسم المؤلف: وسن فوزي عبد الحسین

دور مستارجات حشرتي الصرصر الامريكي Periplaneta mericana والبعوض Culex pipiens على فرط التحسس من النوع الاول وعلاقتها مع بعض الائل مستضدات معقد التوافق النسيجي من الصنف الثاني

المستخلص: This study aimed to verify the distribution of Periplaneta americana and Culex pipiens allergy and its association with some HLA class II alleles in asthmatic and allergic patients. 96 healthy (control ) and 96 suspected allergic individuals from the same geographical region ,paired by sex and age were included in this study. Enzyme Linked Immunosorbent Assay test (ELISA) was used to estimate the total and specific IgE antibodies in the sera of studied individuals. The detection of the HLA - DQB1*0602, HLADQB1* 0604 and HLA - DRB1*12 was performed by Polymerase Chain Reaction (PCR).The results of total IgE based ELISA which was conducted on 96 suspected allergic and24 healthy (control ) individuals revealed that there was extremely significant statistical association (P<0.0001) for total IgE concentration with being allergic or non allergic status of individuals. The overall frequency of higher total IgE antibody concentration ( IgE>200IU/ml) was observed in 72.9% of allergic patients and lower serum total IgE levels (IgE<200IU/ml) were associated with 87.5% of healthy individuals. According to the specific IgE based ELISA results the effect of allergic patients age on the IgE seropositivity against cockroach allergens was not considered to be significant(p>0.05),but the effect of sex was statistically significant)P<0.05(.There was no significant effect( p>0.05)for patients age and sex on the distribution of IgE antibodies against mosquito allergens. Out of 96 allergic patients, 59 (61.5%)and 62(64.6%)showed positive IgE antibody response against cockroach and mosquito allergens respectively. Concerning the PCR results the overall frequency of HLA - DQB1*0602 and HLA - DQB1*0604 alleles was %35.6 and %15.3 of cockroach allergic patients respectively and the HLA - DRB1*12 allele was not observed in these patients..Out of 62 mosquito allergic patients, 8(%12.9)showed HLA - DQB1 * 0602 positive results while HLA - DQB1*0604 allele was observed in 4(%6.5)patients and the HLA - DRB1*12 allele was not observed in these patients.The difference in the genotypig results was extremely highly significant(χ2 : 0.030;17.486; degrees of freedom(DF) : 3;p - value=0.00056136) between cockroach and mosquito allergic patients .The association between the presence HLA - DQB1 *0602 , HLA - DQB1 * 0604 and HLA - DRB1*12 and total IgE based ELISA results in allergic patients and healthy individuals revealed that the percentage of HLA - DQB1 * 0602, HLA - DQB1 * 0604 and HLA - DRB1*12 alleles presence in patients having total IgE lower total IgE )IgE<200 IU/ml) was higher than that of high )IgE>200 IU/ml) total IgE patients where HLA - DQB1*0602 appeared in high percentage(69.2%) followed by percentage (15.4 %( of HLA - DQB1* 0604 presence. . The relationship of HLA - DQB1and HLADRB1 alleles with the results of total IgE based ELISA was statistically significant in the least(χ2 : 60.756 and the degree of freedom (DF) : 5;p= P<0.00001) and greatest(χ2 : 11.806 ; the degree of freedom (DF) : 5 ; p = 0.037544) than 200 IU / ml, values .Depending on the association between the presence of HLADQB1* 0602 ,HLA - DQB1*0604 and HLA - DRB1*12 and positive or negative specific results of cockroach or mosquito allergens specific IgE based ELISA results in allergic patients revealed that the percentage of HLA - DQB*0602,HLA - DQB1*0604 and HLA - DRB1*12 alleles presence in cockroach allergic patients having positive results were higher than those ratios that have been observed in patients with negative ELISA results where HLA - DQB1*0602 appeared in high percentage)35.6%( followed by percentage( 15.3% )of HLA - DQB1*0604 . The relationship of HLADQB1and HLA - DRB1 alleles with the results of specific IgE based ELISA was statistically significant (χ2 : 10.412; DF : 3; p - value =0.01536989).Incontrast the percentage of HLA - DQB1*0602,HLADQB1*0604 and HLA - DRB1*12 alleles presence in mosquito allergic patients was found to be higher in negative ELISA results patients also the relationship of these HLA - DQB1 alleles with mosquito allergen specific IgE based ELISA results was not considared statistically significant (χ2 : 3.115 ;DF : 3 and p= 0.37423205).However HLA - DQB1 * 0602 allele was found in the highest percentage (20.6%), followed by allele HLA - DQB1 * 0604 (8.8%).Concerning the presence of HLA - DQB1*0602 ,HLA - DQB1*0604 andHLA - DRB1*12 in cockroach or mosquito allergic patients and healthy individuals,the HLA - DRB1*12 and HLA - DQB1*0602 allels were not found in healthy individuals.The genotyping results difference between cockroach allergic patients and healthy individuals was considered statistically extremely significant (χ2 : 74.485 ;DF : 5 and p =0) as well as the difference in the results of the genotyping of mosquitoes allergic patients and healthy people was statistically with very high significant (χ2 : 23.342 ; DF : 5 and p = 0.00029035).

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: ماجستير

الموضوع الدقيق: علوم الحياة

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: فوزية علي عبد الله ضياء خليف كريم

اسم المؤلف: هدى كاظم كريم الموسوي

استخدام الاسماك الذهبية Carassius auratus كدلائل حيوية لتلوث هور الحمار في البصرة

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم

الدرجة: ماجستير

الموضوع الدقيق: البيئة - التلوث البيئي

السنة: 2015

الموضوع العام: علوم الحياة

اسم المشرف: اياد حنتوش داود الخفاجي

اسم المؤلف: هدى حسن خربيط الخيون

دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية : دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية والوراثية عند العاملين في معمل البتروكيمياويات في محافظة البصرة والوراثية عند العاملين في معمل البتروكيمي The Study of the effects of previous Ethylene Exposure on Some Physiological and Genetic Parameters in Workers of Petrochemical Plant In Basrah province

المستخلص: The current study was conducted to investigate the genetic and physiological effects of some industrial chemicals on workers of the Petrochemicals Industrial Factory, in Basra Province , as it has been collecting 100 blood samples during the month of January and February of 2011 from each of the exposure in (ethylene section) and the control group of (employees of the University of Basra). and then the samples were divided on the basis of each of the years of occupational exposure to more and less than 15 years , age group to the larger and smaller than 50 years, smoking habits.The results showed that there was a significant decrease in the haemoglobin concentration HB, PCV, RDW and MCV in the more than15 years exposure group in comparison with the control group, while, no significance was detected in the RBC, MCH, MCV ,RDW,MCH and MCHC in less than15 years exposure group . Whereas, a significant decrease in the PCV for the same group was observed.White blood cells parameters’ results showed also variations in their values. In general, WBC did not change significantly in both groups of workers, while, the proportion of the lymphocyte and the of monocyte declined significantly in the more than 15 year of occupational exposure group, in contrast, the proportion of granulated cells increased markedly in the same group. The results of group of less than 15 years exposure revealed that the proportion of monocyte dropped significantly. Conversely, there was a significant increase in the proportion of granulated cells while the proportion of the lymphocyte was not significant . Platelets’ number and average of platelets’ volume decreased significantly in both occupational groupsTurning to the biochemical tests, the results showed significant increase in both liver enzymes ALT ,ASTand bili as well as this increase was found in urea, uric acid and glucose values in both occupational exposure groups. Contrariwise, the ALP creatinine and cholesterol did not reveal significant changes in both groups that in comparison with control group. The total protein decreased significantly in the group of more than 15 year of occupational exposure.Testosterone level revealed a significant decline in both occupational groups, while the Follicle stimulating hormone showed a significant increase in the occupational group of less than 15 year and no difference was observed in another occupational group while LH hormone level was not significant.In case of the genetic effects of the ethylene compounds, DNA was extracted for control and occupational groups and amplification was performed for GSTM1, GSTT1 and Albumin by using PCR technique. The results showed that there was no significant effect of both occupational periods on the GSTM1 gene, while GSTT1 gene was significantly in the workers samples in group more than 15 year of exposure, which was 2.43 times more than the group of less than 15 year of exposure.As regards the impact of exposure to ethylene and loss of genes GSTM1 and GSTT1 showed the results of our study, significant increase in the loss of gene GSTM1 amounted to more than five - fold (5.1) among workers exposed compared to a set of control while the proportion of loss of gene GSTT1 by more than three - fold (3. 1) compared to the group control has been a loss ratio of the two genes together more than eight - fold (8.5) for workers exposed group compared to the control.The light smoke did not reveal a significant effect on the null of GSTM1 and GSTT1 genes that in comparison with the control group, while the study found a significant effect of smoking on gene GSTM1, causing the null of much more than doubled (2.2) times compared with the control group (non - smokers) and the significant effect of smoking on null GSTT1 gene loss as increased visits by the text (1.5) compared with non - smokers also appeared that excessive smoking has an effect on the GSTT1 gene causing loss by seven - fold (7.08) once compared to non - smokers.The effect of the age group (less and more than 50 years old) of the workers on the null of both GSTM1 and GSTT1 was also examined. GSTM1 gene was null six times in both age groups of workers more than the control samples. While the GSTT1 was null in age group less than 50 years old three times more than the control group. As well as this absence was two times in the age group more than 50 years old in comparison with control group.It could be concluded from the present study that the exposure of workers to ethylene and its metabolic impact and clearly on blood standards as well as caused a high level of enzymes AST, ALT and bilirubin and high blood sugar levels and lower total protein and high level of urea and uric acid level as well as the low level of the hormone testosterone as it turns out that occupational exposure to ethylene and metabolic outcomes and length of exposure and smoking for workers exposed role in influencing the genes of detoxification, causing null in each of the gene GSTT1 and GSTM1.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: علوم الحياة

السنة: 2015

الموضوع العام: علوم الحياة

اسم المشرف: سامي جبر المالكي فائزة عبد الوهاب احمد

اسم المؤلف: هبة ثاقب يسر

دراسة مظهرية وجزيئية وبيئية للدور اليرقي بعد المذنبة لمثقوبة العين Diplostomum على ثلاثة انواع من اسماك نهر كرمة علي في محافظة البصرة Morphological, Molecular and Ecological Studies of the larval stage (metacercaria) of eye trematode Diplostomum on three fish species from Garmat Ali River, Basrah Province

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: ماجستير

الموضوع الدقيق: علوم الحياة

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: عبد الحسين حبش

اسم المؤلف: هاجر رزاق منهل

المستخلص: The present study was concerned with some biological aspects of the invasive snail Pomacea canaliculata (Lamarck, 1822) and for this purpose, monthly samples from Shatt Al - Arab banks in the area of Al - Salhia were collected during the period from October 2014 to September 2015. The study included the monthly variations in the density of snail , its egg masses and the population sizes. In the laboratory, the study included many aspects, these are the relationships between female weight with some measurements of its egg masses, embryonic development duration, hatching percentages under different temperatures, food consumption, salt acclimatization, growth under combined effect of salinity and temperature, effect of food type on growth of newly hatched snails and examine samples of snail to detect the infection with parasites if present. The density of the snail was ranged from four to 13 ind. /m2 recorded during January - February 2015 and October 2014 respectively, while the mean density of egg masses was from two to nine egg mass / m2 recorded during April 2015 and October 2014. The statistical analysis showed that the temperature had positive correlated with density of each of snails and its egg masses. On the otherhand, there are positive correlation between the density of each snail and its egg masses. The snails, which had been collected during the study period, were 19 - 68 mm in length, these measurements were classifying into five size classes. The size class 35 - 45 mm was dominanted for nine months, compared with the size class 55 - 65 mm that had less occurrence.The diameter of eggs varied from 2.5 - 4 mm. The 25°C is the most suitable temperature to get a high percentage of hatching and a short period of embryonic development. There was no significant correlation between the weight of the female and the number of its egg masses.The feeding experiment showed that the amount of food consumed lettuce mild during the study period 30 days by one individual ranged from 3 - 29g. The results of the acclimatization was referred to the ability of all size classes' snails to tolerate the low salinity 2.5 psu with 100% survival percentage, while the snails of size classes at least 10 mm and 10 - 25mm were able to tolerate the salinity of 11psu with 10% and 50% respectivly survival.There are variable effects of the combination of temperature and salinity on growth of newly hatched snails for 9 weeks experiment, these effects were reflected on the weight, length and width, and the highest of these measurements were recorded for snails of 2.5 psu - 27°C combination, while the lowest one was recorded in 7.5 psu - 10°C. The lowest survival percentage was 5% recorded for the individuals of 7.5 psu - 27 and 32°C, compared with 90% of distil water - 17°C. On the other hand, the optimum growth was in 2.5 psu all temperatures, while the optimum growth was in all salinities, and temperature of 27°C.The experimental study of five types of food fresh celery, fresh lettuce, fish food, mixed food and animal protein)on growth of newly hatched snails for 9 weeks. Showed that the mean weight, length and width of snails were depending on the food type. The mixed food gave significant increase in growth 382.1 mg weight, 10.10mm length and7.58mm width compared with the other types of food.The highest survival percentage 95% was recorded for the snails fed on celery, lettuce and fish food. The present study on the snail P. canaliculata does not record any infection by the larvae of parasites

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: ماجستير

الموضوع الدقيق: علوم الحياة

السنة: 2016

الموضوع العام: علوم الحياة

اسم المؤلف: نادية جعفر كاظم البغدادي

تركيز بعض ملوثات الهواء في بعض المناطق الصناعية والحضرية من محافظة البصرة العراق Concentration of some air pollutant in some industrial and urban areas from the province of Basrah - Iraq

المستخلص: The current study was conducted, from October 2013 to September 2014, aimed to identify the temporal and spatial variations of air pollutants in stationary sites included Basrah oil refinery and southern gas company in addition to sources included intersections traffic in Basrah province (Alkzizah, Tigari and Jazaier streets).The results showed that in Basrah oil refinery the CO and SO2 concentration were less than the national and World Health Organization standards, while NOX were higher than the national and global standards reached 1ppm.The second site, which is southern gas company was as follows : CO gas was less than the national and global standards as well as NOX, while SO 2 was higher than the national and global standards reached 0.63 ppm.For intersections traffic sites (intersections)the results was as follows : CO was less than the national and global standards, while NOX was higher than the national and global standards reached 2.75ppm as well as SO2 at all intersections reached 0.65 ppm.Ambient air quality was recorded through the seasons of the year using the accurate measurement devices in various study sites of the province of Basrah. The results were indicated that the concentration of all the gases rate was higher in the winter than those recorded in summer due to the difference in climatic conditions, especially wind speed and direction, air temperature and moisture.The results showed a temporal and spatial variations in concentrations of studied air pollutants in ambient air interchanges traffic roads within the province of Basrah centerdue to difference in the rush hours , the activities of the various rights in traveling or shopping as well as the routine jobs procedure of the official working hours.The study showed variation in the concentrations of total suspended particles and black carbon among studied traffic intersection recorded highest concentration of black carbon at Quzaizah amounted to 27 μg/ m3 As the TSP recorded less concentrations 392 μg/ m3. But at the intersection of Tijari Street has concentrations of black carbon amounted to 11.3 μg/ m3 ,As for particles recorded 412 μg/ m3, either at the intersection of Jazair Street was recorded black carbon of 16.2 μg/ m3, As for particles amounted to 420 μg/ m3.All intersections have exceeded the global limitations of particles amounted of 150 μg/ m3 and local of 350 μg/ m3It can be concluded from the current study that the province of Basrah are polluted by air pollutant NOX gas in Basrah oil refinery and SO2 in the site Southern gas company NOX and SO2 at all intersections traffic sites located within the current study.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم - قسم علوم الحياة

الدرجة: ماجستير

الموضوع الدقيق: البيئة - التلوث البيئي

السنة: 2014

الموضوع العام: علوم الحياة

اسم المشرف: نايف محسن عزيز

اسم المؤلف: معتز حكمت عثمان

تاثير بعض المعاملات في زيادة تحمل نبات الموز (Musa acuminata L.) الصنف الهندي للشدين الملحي والمائي باستعمال تقانة زراعة الانسجة النباتية Effect of some treatments on increasing salinity and water stress on Musa acuminata L. (Hindi cultivar) using plant Tissue Culture techniques

المستخلص: اجريت الدراسة الحالية في المختبر التقني لزراعة الانسجة النباتية التابع للقطاع الخاص الواقع في منطقة الفيحاء / قضاء شط العرب/ محافظة البصرة للفترة من 2012/12/20 ولغاية 2014/12/20 على نبات الموز الصنف الهندي قصير الساق Musa acuminata L. بهدف وضع برنامج للاكثار الدقيق ومدى تحمل النبيتات للشد الملحي باستعمال كلوريد الصوديوم والشد المائي باستعمال مركب الكلايكول متعدد الاثيلين (PEG)Polyethelen glycol ومعرفة تاثير معوق النمو الباكلوبيوترازول (PBZ)Paclobutrazol في تضاعف الافرع الخضرية وفي اقلمة النبيتات وتاثير الهرمون النباتي حامض الابسيسك (ABA)Abscisic acid في تحسين التحمل للشدين الملحي والمائي. استعمل انصاف من القمم المرستيمية النامية Shoot tips كاجزاء نباتيةExplants على وسط MS مضافا اليه 5ملغم.لتر1 - من السايتوكاينين (BA)Benzyl adenine لتحفيز تضاعف الافرع الخضرية ومعاملتها بتراكيز مختلفة من ملح كلوريد الصوديوم وPEG وPBZ وABA وتم حساب عدد الافرع الخضرية الناتجة وقطرها وارتفاعها وعدد الجذور واطوالها ومتابعة نموها ثم اجراء عملية الزراعة الثانوية لعدة مرات ثم اقلمتها ونقلها الى البيت البلاستيكي.ويمكن تلخيص النتائج التي تم الحصول عليها بما يلي : 1 - اضافة ملح كلوريد الصوديوم بالتراكيز 0 او 20او50 او80 او120 ملي مولر الى الوسط MS اثر في تضاعف الافرع بنسب متفاوتة , وادى الى زيادة معنوية في المدة اللازمة لظهور الافرع الخضرية وتقليل عددها بزيادة تركيز الملح .2 - اضافة معوق النمو PBZ بالتراكيز 0 او0.5 او 1 او1.5 او2 ملغم.لتر1 - بوجود السايتوكاينين BA بالتركيز 5ملغم.لتر1 - ادى الى تقليل المدة اللازمة لبدء ظهور الافرع الخضرية وزيادة عددها وخفض ارتفاع النبات وعدد الجذور بزيادة تركيز PBZ.3 - تفوقت المعاملة الاولية بـPEG بالتركيز 1% معنويا على التركيز 0.5% في عدد الافرع الخضرية وفي عدد الجذور وطولها وزيادة في ارتفاع النبات وقطره .4 - اضافةABA الى الوسط الغذائي بالتركيزين 0.1 او0.3 ملغم.لتر1 - ادت الى تقليل عدد الجذور واطوالها وارتفاع النبات وقطره وتيبس الاوراق واصفرارها مقارنة مع معاملة السيطرة بينما تفوقت معاملة التداخل مع تراكيز كلوريد الصوديوم المدروسة مع المعاملتين 20و50 ملي مولر من كلوريد الصوديوم تفوقا معنويا على المعاملتين 80 و120 ملي مولر في زيادة عدد الجذور واطوالها وطول النبات وقطره . 5 - سببت اضافة كلوريد الصوديوم الى الوسط الغذائي زيادة معنوية في تركيزي ايوني الصوديوم والكلورايد وهبوط معنوي في تركيز ايون البوتاسيوم ونسبة البوتاسيوم /الصوديوم بينما ادت اضافة PBZ وPEG وABA الى انخفاض معنوي في ايون الكلورايد وزيادة معنوية في نسبة البوتاسيوم /الصوديوم .6 - سببت المعاملة بملح كلوريد الصوديوم زيادة معنوية في محتوى الاوراق من البرولين بزيادة تركيز الملح .كما ان تداخل تراكيز ملح كلوريد الصوديوم مع PBZ وPEG وABAقد سجلت زيادة معنوية في محتوى البرولين بزيادة تركيز الملح . 7 - ادت المعاملة بملح كلوريد الصوديوم عند التركيزين 20و50 ملي مولر زيادة محتوى الاوراق من الكربوهيدرات الذائبة الكلية في حين ادت المعاملة بالتركيزين و80 و120 ملي مولر الى هبوطها.اما بالنسبة لتداخل تراكيز ملح كلوريد الصوديوم مع تراكيز PBZ وPEG وABA فقد تفوقت معنويا في محتوى الاوراق من الكربوهيدرات عند جميع التراكيز مقارنة مع معاملة السيطرة .8 - اوضحت النتائج ان محتوى الاوراق من الكلوروفيل الكلي قد انخفض معنويا عند تركيزي80 او120 ملي مولر كلوريد الصوديوم .كما ان المعاملة بـ PBZ وPEG وABA ادت الى زيادة محتوى الكلوروفيل الكلي عند جميع التراكيز .9 - اقلمت النبيتات الناتجة من الافرع الخضرية المزروعة في الوسط الغذائي المزود بتراكيز ملح كلوريد الصوديوم فقط ومن تداخل كلوريد الصوديوم مع تراكيز PBZ وPEG وABA وقيس ارتفاع النبات وقطره وقورنت مع معاملة السيطرة .10 - ادت المعاملة بتراكيز PBZ وPEG وABA المتداخلة مع تركيزي ملح كلوريد الصوديوم 20 و50 ملي مولر الى خفض معدل النسبة المئوية للرطوبة المفقودة وزيادة النسبة المئوية للنباتات المؤقلمة.11 - اضافة كلوريد الصوديوم الى الوسط الغذائي ادى الى حدوث تغيرات في عملية التعبير الجيني وظهور اختلافات في عدد ومواقع وكثافة الحزم البروتينية .12 - التداخل بين كلوريد الصوديوم والمواد المضافة PBZ وPEG وABA ادت الى اختفاء بعض الحزم وظهور حزم اخرى في مواقع مختلفة خصوصا الحزمتين 3و 4 . | This study was conducted in the laboratory of the technical plant tissue culture in Fayhaa / Shatt Al - Arab District / province of Basra for the period from 12/20/2012 till 20/12/2014 at Dwarf banana cv. Indian Musa acuminata L. In order to develop a program for micropropagation and were to investigate salt stress tolerance through the effect of NaCl, and water stress through Polyethelen glycol(PEG) and knowledge of the growth retardant of Paclobutrazol (PBZ) in multiplication of the branches vegetative and acclimatization and the effect of Abscisic acid(ABA) on improving salt and water stress tolerance. Halves of shoot tips were used explants which were cultured on MS medium supplied 5 m.l - 1 of Benzyl adenine (BA) to stimulate the multiplication of the branches vegetative and treated with different concentrations of sodium chloride and PEG and salt PBZ and ABA were calculating the number of branches vegetative resulting in diameter and height and number roots and length of follow - up and growth of agriculture and then conduct subculture for several times and then acclimatized and transferred to the plastic house.The results of this study can be summarized as follows : 1 - The addition of 0 or 20 or 50 or 80 or 120 mM of sodium chloride to the MS medium affected the multiplication of the branches vegetative in various percentages. This, in turn, resulted in a signficant increase in the required time to initiate the adventitious buds and lowered their number. There was also a significant difference in the percentage of increasing the numbers of those branches by increasing the salt concentrations. 2 - The addition of the concentrations 0 or 0.5 or 1 or 1.5 and 2 mg.l - 1 of growth retardant PBZ together with 5 mg.l - 1 BA resulted in lowering the required time for initiating the adventitious buds, increasing their number, decreased the number of roots and height of stem by increasing the concentration of PBZ.3 - The initial treatment by 1% PEG significantly increased than the concentration 0.5%. This increase included the number of the adventitious buds, number and length of roots, as well as height and diameter of stem.4 - The addition of 0.1 and 0.3 mg.l - 1 ABA to the nutrient medium led to lower th number and length of roots, stem height and diameter. The leaves became dry and yellow when compared to the control treatment. As far as the interactions between the sodium chloride concentrations in question are concerned, the two concentrations 20 and 50 mM showed a more significant difference than the other concentrations 80 and 120 mM in the number and length of roots, and stem length and height.5 - The addition of the sodium chloride to the nutrient medium resulted in a significat increase in the concentrations of sodium chloride ions and a significant decrease of potassium/ sodium percentage. The addition of PBZ, PEG, and ABA resulted in a significant decrease in chloride ion and a significant increase in potassium/ sodium percentage.6 - The sodium chloride treatment caused a significant increase in the leave content of Proline by incresing the salt. The interaction between sodium chloride salt concentrations and PBZ, PEG, and ABA showed a significant increase in the proline content by increasing salt concentration.7 - The treatment by the two concentrations 20 and 50 mM sodium chloride resulted in increasing the leave content of the total soluble carbohydrates, whereas the treatment by the two concentrations 80 and 120 Mm lowered the leave content. Considering the interaction between sodium chloride and PBZ, PEG and ABA, the interaction showed a significant increase in the leave content of carbohydrates at all concentrations compared to the control treatment.8 - The results showed that the leave content of chlorophyl increased significantly at the two concentrations 20 and 50 mM sodium chloride. Also, the treatment by PBZ, PEG, and ABA showed an increase in the chlorophyl content at all concentrations.9 - The resulted plantlets were acclimatized by sodium chloride salt concentrations and by the interaction of Sodium Chloride and PBZ, PEG, and ABA. The plant height and stem diameter were measured and compared to the control treatment.10 - The treatment by PBZ, PEG, and ABA concentrations that are interacted with Sodium Chloride salt concentrations at 20 and 50 mM led to lower the average percentage of lost moisture and increase the percentage of the success of acclimatization. 11 - Add Sodium chloride to the media has led to changes in the process of gene expression and the emergence of differences in the number and location and intensity of the protein packets.12 - The interaction between the sodium chloride and additives PBZ and PEG and ABA led to the disappearance of some packages and the emergence of other packages in different locations, especially packages 3 and 4.

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية

الدرجة: دكتوراه

الموضوع الدقيق: علوم الحياة

السنة: 2015

الموضوع العام: علوم الحياة

اسم المشرف: حسين خلف زاير عباس مهدي جاسم

اسم المؤلف: مريم جاسم محمد

دراسة المسببات الميكروبية الشائعة للاسهال لدى الاطفال دون خمس سنوات من العمر وتوصيف عاثية الامعاء

المستخلص: The purpose of present study was to detection of Rotavirus A and Escherichia Coli from diarrhea cases in children, and characterization of E.coli phag, in addition to study some of factors that influence on Diarrhea.Stool samples were collected between 15/11/2014 and 1/4/2015 from children 0 to 59 months of age who were hospitalized in Basra hospital for women and children, Basra/Iraq. A total of 300 children with acute gastroenteritis were enrolled, including 199 males and 101 females.In this study among children with Diarrhea was 93/300 (31%) Rotavirus positive cases by Immunochromatographic (IC) test as monoinfection (1.66%), coinfection (26.34%), and mixing infections (3%). Out of 50 IC positives fecal samples were tested using electronic microscope, 50(100%) were found positive. A total of 80 stools were examined for Rotavirus using polyacrylamide gel electrophoresis. The overall agreement was 68/80(85%).Furthermore, regardless of the types of infection, monoinfection or coinfection or mixing, Bacteria was isolated, including; 277/300 (92.33%) children had infections with Escherichia coli, 39/300 (13%) cases with Salmonella spp., While, Shigella spp. was reported in 12/300 (4.01%) samples. The identification of bacterial pathogens was later confirmed by Api 20 Enterobacteraceae system. Also parasitic causes were found in 6/300 (2%) samples.Overall, this study recorded 182 /300 (60.66%) of monoinfection cases including; 5 /300 (1.66%) with Rotavirus, 163 /300 (54.33%) with Escherichia coli, 6 /300 (2%) with Salmonella spp., 2 /300 (0.67%) with Shigella spp., 3 /300 (1%) with Entamoeba histolytica, 3 /300 (1%) with Giardia lamblia. Coinfection with another microorganism was observed in 109/300 (36.34%) cases, coinfection with Rotavirus and Escherichia coli were the most common and occurred in 75/300 (25%) cases.Antibiotic susceptibility testing was performed on Muller Hinton agar against five different antibiotics to 30 isolates of E. coli. The antibiotic resistance/susceptibility profile of E. coli isolates revealed that most of the isolates were resistant to three tested antibiotics (Trimethoprim, Ampicillin and Doxycyclin). The phage ɸEC - MH1 was isolation successfully from sewage. The phage titer was determined by serial dilution (10 - 1 to 10 - 9) of the sample by counting the number of plaque forming units (p.f.u.) for each dilution.Results revealed that dilution factor 10 - 2 was the best countable number of plaques. The statistical analysis was significantly decrease P≤ 0.05 in phage titer at the temperature 50C° and 65C° during different times comparing with phage titer at the temperature 37C°. Effects of chloroform on phage titer during different times were completely inactivated comparing with saline environments. Furthermore, regarding some clinical information; the present study showed that 66.33% of the males were more susceptible to the infection with highly significant (P<0.001) than females (33.67%). Mean age (mean ± standard deviation) of all positive cases was 11.02±12.3 months, and the age periods between (0 - 5) and (6 - 11) were significantly prone to the infection.Also, all cases were complained with diarrhea (100%), fever (82.34%), Vomiting (43%) then dehydration (2.67%). In addition, all positive cases become increased in December (32%) and city center (81%). This study showed positive cases increase (69%) of children with artificial feeding

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: ماجستير

الموضوع الدقيق: الاحياء المجهرية

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: كوثر هواز مهدي حيدر عبد الحسين مكلف

اسم المؤلف: مروة محمود يعقوب

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية التربية للعلوم الصرفة

الدرجة: دكتوراه

الموضوع الدقيق: الحيوان - الحشرات

السنة: 2015

الموضوع العام: علوم الحياة

اسم المشرف: ضياء خليف كريم

اسم المؤلف: علاء ناظم حاتم علي

دراسة الطفيليات الداخلية والخارجية في وعلى انواع مختلفة من الحشرات

المستخلص: Present study was carried out to isolate and identify the worms nematodes found in the soil, and could be used as a means of biological control against insects, it has also been to isolate and identify Mites present on or within different types of economic and other insects, which were represented by the American cockroaches Periplaneta americana ( Dictyoptera; Blattiidae). And houseflies Musca domestica (Diptera : Muscidae), Persian and ants belonging to the family; Formcidae) Hymenoptera), flour beetles Tribolium castaneum (Tenebrionidae; Coleoptera), and the termites Microcerotermes diversus( Isoptera; Kalotermitidae))The study samples (soil and insects)were collected from different regions and ecosystem of the province of Basra, during the period of the month of December 2014 to the month of September 2015. By setting traps, appropriate and specifically designed for this purpose, that supplied with special food to attract nematodes in the soil and catch insects found in those ecosystem, . and also to determine the best ways to isolate samples preserved and propagate them..Also, the study dealt with experiments for the propagation of the mites (which collect from insect samples) in the laboratory,by growth them on different growth media, for the purpose of immunological and genetic study, and the ability to be used in bio resistance and also to determine the sensitivity of the laboratory mice, that attacked by the mites through conducting hitological study of the skin of these animals..Results recorded two types of endo parasitic nematodes these areFamily : Steinernematidae1 - Steinernema feltiae2 - S. carpocapsaeAnd five species of mites, these areFamily : Histiomatidae2 - Histiostoma sp1 Family : Acaridae 1 - Cosmoglyphus barbisetusgrifolapholiotae 2 - Mycetosancassania Family : Macrochelidae Vitzthum, 1930 Macrocheles muscaedomesticae Family : Pyemotidaepymephorus tarsalis

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم - قسم علوم الحياة

الدرجة: ماجستير

الموضوع الدقيق: علوم الحياة

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: كاظم صالح حسن الهدلك مسلم عبد الرحمن الطعمة

اسم المؤلف: علا عبد الشهيد ناصر

المستخلص: ELISA technique was used to estimate of histamine in muscles of several types of local fresh fish, including : Tenualosa ilisha, Megalaspis cordyla , Chirocentrus dorab and Scomberoides commersonianus in addition of the shrimp Metapenaeus affinis , all samples of fish and shrimp were collects from Fao markets in Basarah city .Results showed the percentages of histamine in all types of fish, as well as shrimp were : 4.483 and 3.953 and 3.353 and 3.16 and 2.03 mg/kg respectively.Accumulation of histamine in fish after refrigerating for three days was also determinated. Results showed that the values of histamine accumulate were increased within preservation period .On the third day of histamine accumulate of C. dorab , T. ilisha , M. cordyla , S. commersonianus and shrimp were 4.56 , 3.99 , 3.43 , 3.19 and 2.01 mg / kg, respectively. While , on the first day were 4.01 , 3.65 , 3.03 , 2.98 and 1.89 mg/kg respectively .ELISA technique was also used to estimate histamine in Ripening cheese such as cheddar , Alkuda , Almoszerla and Oloadam cheese and smoked Hajdu . They were 4.267 , 2.433, 1.167 , 3.667 and 1.833 mg / 100 g , respectively.Many bacteria were isolated isolated from fishes and shrimp, they identified according to : growth characteristics on selective media, morphological, and microscopic examinations in addition to biochemical tests.Fifty three isolates of bacteria were found, they were : Klebsiella pneumonia sub. pneumonia , Klebsiella oxytoca , Morganella morganii sub. Morganii, Enterobacter aerogenes, Enterobacter cloacae , Cronobacter sakazakii ,AEnterobacter taylorae, Citrobacter freundii , Citrobacter amalonaticus , Proteus marina, Proteus vulgaris , Proteus mirabilis, Hafnia alvei , Escherichia coli, Serratia marcescens, Serratia fonticola , Vibrio vulnificus, Vibrio diazotrophicus , Vibrio hollisae , Vibrio alginolyticus, ,Vibrio cholera, Vibrio parahaernolyticus, Vibrio fluvialis, Lactobacillus helveticus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus johnsonii, Lactobacillus salivarius, Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus saprophyticus, Staphylococcus aureus , Staphylococcus intermedius , Staphylococcus epidermidis, Bacillus pumilus , Bacillus licheniformis , Bacillus firmus , Bacillus subtilis, Bacillus alvei , Aeromonas. caviae , Aeromonas veronii bv.veronii , Aeromonas eucrenophila, A. sobria , Aeromonas veronii and Aeromonas encheleia .Isolation and identification of Lactobacillus from all samples of ripening cheese were done. Six species of Lactobacillus were found and as a follow : Lactobacillus plantarum , Lactobacillus delbrueckii, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus fermentum and Lactobacillus paracasei .DNA was extracted from all types of G+ and G - bacteria which isolated from fish and shrimp in addition to Lactobacillus bacteria that isolated from ripening cheeses . the DNA of all bacteria was good and pure .Polymerase chain reaction (PCR) technique was used for identification all isolates of Lactobacillus which were isolated from fish and shrimp, as well as ripening cheese depending on the bacterial 16S rRNA gene sequences of bacteria with using different types of diagnosed primers, genes had been identified for : Lactobacillus acidophillus , Lactobacillus plantarum , Lactobacillus delbrueckii , Lactobacillus fermentium , Lactobcillus helveticus , Lactobcillus crispatus ,BLactobcillus gasseri, Lactobcillus reuteri , Lactobcillus rhamnosus, Lactobcillus paracasei, Lactobacillus johnsonii , Lactobacillus salivarius and Lactobacillus casei .Nine primers were in RAPD technique to find out the genetic link between different strains of Lactobacillus bacteria, the results showed that the number and location of randomized bands were differed in some bacterial species but others was fitted and produced a variety genetic of bacterial strains, in particular a number of bands as possible to get a variety of different genetic profiles among Lactobacillus isolates .The results appeared different styles of bands and presence of specific bands in Lactobacillus bacteria in all primers . The results obtained from RAPD technique showed that the total number of bands were 630 bands, the total number of bands which formed genetically was 127 and the percentage of total proportion of genetic variation among the isolates was 20.15% .The results found of genetic primers scheme that the genetic distance was as close as possible between bacteria L.fermentum and L.rhamnosus and was a part of the one genetic group, but L.helveticus was close as possible genetically to this group , and then the L.johnsonii and L.acidophilus was more genetically distant to this group. Also it found that the genetic distance was very near between L.plantarum and L.paracasei and were a part of a one hereditary group and found also that L.reuteri closest genetically to this group, then followed by more genetically L.delbrueckii . However, L.acidophilus was also more genetically distant to this group .CThe results revealed that L.salivarius and L.crispatus were a one hereditary group and the genetic distance for this group was very close to the L.gasseri and it was farther genetically for genetic group, which includes L.fermentum and L.rhamnosus , also found that the bacteria L.casei showed far genetic distance between them and the groups of Lactobacillus .All fifty three isolates of bacteria which isolated from fish , shrimp and ripening cheeses that producing and did not producing histamine subjected to amplified genes which responsible of the histamine production by using the primers HIS1 - F/HIS1 - R ; JV16HC/ JV17HC ; Hdc - f/ Hdc - r ; 106/107 and UNI - L/ UNI - R . Amplification results showed that all primers used to amplified genes of histamine gene different genes sizes depending on the types of bacteria when were positive or negative to Gram stain and their OrigenIt was found the size of the gene was 350 base pairs , was found in G+ bacteria which isolated from fish , shrimp and cheese ripening . These bacteria included : L.acidophilus ,L.helveticus ,L.crispatus, L.gasseri ,L.casei , L.reuteri , L.fermentum,L.rhamnosus, L.paracasei, L.johnsonii, L.salivarius L.plantarum , L.delbrueckii , S.aureus , S. saprophyticus , S.epidermidis , S. intermedius , Bacillus pumilus, B.subtilis, B.licheniformis, B.firmus and B. alvei .May same results were found when amplified HISI - R/HISI - F primer , the size of histamine gene most positive Gram bacteria stain .High size of histamine gene 709 base pair appeared when amplified Hdc - r/ Hdc - f primer .This gene was found in G - bacteria which isolated from fish and shrimp . These bacteria included : Pseudomonas aeruginosa , Pseudomonas fluorescens, Klebsiella pneumonia , Morganella morganii , Enterobacter aerogenes, KlebsiellaDoxytoca, Hafnia alvei, Enterobacter cloacae, Vibrio alginolyticus, Cronobacter sakazakii, Escherichia coli , Enterobacter taylorae, Proteus vulgaris, Proteus mirabilis, Vibrio vulnificus , Vibrio diazotrophicus, Vibrio hollisae , Vibrio fluvialis , Serratia marcescens and Serratia fonticola .However , the size of histamine gene was 530 base pairs , when amplified 106 / 107 primer , the gene was found in most G - bacteria .On the other hand , the UNI - L/UNI - R primer did not amplify in all isolates of bacteria which isolated from fish , shrimp and ripening cheeses .Extraction of plasmids from all isolated bacteria was done . Results showed that isolates have different types of plasmids bands (small and large size) and when the electric deportation of the genetic material of plasmids on gel agarose while some other isolates appeared possess one plasmid.In this study , PCR technique was not detected the presence of histamine genes in plasmid of bacteria , because not occurred any amplify for any type of primers used . This indicates the presence of histamine genes on the genome of the bacteria and not on plasmids of bacteria .

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: العربية

الجامعة: جامعة البصرة - كلية العلوم - قسم علوم الحياة

الدرجة: ماجستير

الموضوع الدقيق: الاحياء المجهرية

السنة: 2016

الموضوع العام: علوم الحياة

اسم المشرف: قيثار رشيد مجيد طالب احمد جايد

اسم المؤلف: صلاح ناجي عزيز الخيون