استخلاص وتنقية وتشخيص ببتيد كلايكوسيدي من الحبار Loligo sp. ودراسة تاصيرة على خط الخلايا السرطانية Hela Cell Line والطبيعية REF == Extraction , Purification, Characterization Glycosidic Peptide From Loligo sp. and Study Effect On Cancer Cell Line (Hela) and Normal (REF)
Author name:
هيفاء عدنان منصور الموسوي
Supervisor name:
بلسم انيس مارينا | ضمياء قاسم سكر
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University Of Basrah - College Of Science - Department Of Biology
Language:
Arabic
University location:
Basrah
First pages:
24T3591 - p.pdf
Abstract:
The study was designated to investigate the capcity of pure Loligo sp extract on two cell lines : - the cancer cell line Human cervix uteri epitheloid carcinoma (Hela) and Rat Embryo Fibroblast cell line (REF) as normal cell line.The study included extraction of tissue (whole body) of Loligo sp by using 30% ammonium sulfate. and chemical group detected in the extract by using qualitative chemical test and the result showed that the crud and pure extract contained alkloides, proteins, carbohdrates, saponines, flavonoids, aldehyde &keton group and phenol group .and the extacte does not contain glycosides.The crude extraction was purified by column chromatography using sephadex G - 25. and the purity of extract detected by using polyacryl amide gel electrophoresis under non denaturated condition. The result showed that the extracted riched with amino acid and some carbohydrate by using high performance liquid chromatography (HPLC) with compared standerd amino acid and suger.Infrared Spectroscopy of purified extract shows that it contain (N - H - C - OH) to emphasize on found amino acid in the extract.The cytotoxicity effect on cell lines study by four concentraction ( 125, 250 ,500 and 1000)ϻg/ml were prepared and tested on cell line with five replicates for each concentraction, the optical density of cell growth read by the Elisa reader 550 nm and use by tetra zolium bromide (MTT). The result for in vitro study showed that all concentraction had high inhibition on tumor cell line and a highly significant inhibition in (Hela) line was recoreded 42.79% at exposure time 48hr in the concentraction 1000 ϻg/ml the effect was dose dependent. The inhibitory effect of extract on Hela cell line higher than in the REF. while it was less effect of extract on Rat Embryo Fibroblast cell line after exposure time 48hr.
