عرض: 25 50 75 100 النتائج

نتائج البحث: 25 من أصل 492

حول التقريب الرتيب للدول متعددة المتغيرات == on Bivariate Monotone Approximation

اسم المؤلف: جنان محمد هادي القيسي
اسم المشرف: ايمان سمير عبد علي بهية
الموضوع العام: علوم الرياضيات
السنة: 2014
الموضوع الدقيق: الرياضيات
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: This thesis deal with the two dimensional problem for monotone approximation.we answer George A. Anastassiou question and we generalize a direct theorem for monotone approximation to the two dimensional theorem for monotone approximation to function in f

الخواص الدينمية لدالة ايكيدا == The Dynamical Properties of Ikeda Map

اسم المؤلف: وفاء هادي عبد الصاحب
اسم المشرف: افتخار مضر طالب الشرع
الموضوع العام: علوم الرياضيات
السنة: 2014
الموضوع الدقيق: الرياضيات
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: في عملنا, درسنا داله ايكيدا وهي من الدوال الغير خطيه في البعد الثالث وصيغتها I_(a,b) ((x@y@t))=(1+a x cost - a y sint@a x sint+a y cos?t@bt)) اعداد حقيقيه a,b حيث درسنا الخواص الدينمية العامه لداله ايكيدا ثم درسنا الخواص الفوضويه لها حيث برهنا ان داله ايك

تاثير قطر وشكل فتحة المسدد على طيف الطاقة لنضيري 137Cs و22Na باستخدام الكاشف الوميضي NaI(Tl) == The Effect of The Diameter And The Shape of The Collimator In The Gamma Ray Energy Spectrum For 137Cs And 22Na Isotopes Using Scintillation Detector NaI(Tl)

اسم المؤلف: مالك صالح محيميد الحمداني
اسم المشرف: خالد حسين هاتف العطية
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تناول هذا البحث دراسة تاثير قطر فتحة المسدد وشكله على طيف الطاقة للنظيرين المشعين (Cs - 137) و(Na - 22) وذلك باستخدام مسددات من مادة الرصاص ذات فتحات في مراكزها باقطار مختلفة, اذ تم اجراء الدراسة باستخدام الكاشف الوميضي [NaI(Tl)] ومنظومة العد والتحليل الا | In the present work, the effect of the diameter, the shape and the position of the collimator in the gamma ray energy spectrum have been studied by using scintillation detector NaI(Tl) of sizes (1 x 1.5) inch and the nuclear counting and analysis system t

امثلية العدسات المغناطيسية ثنائية القطب المتناظرة == Optimization of Symmetrical Double Polepiece Magnetic Lenses

اسم المؤلف: لمياء ميري صالح الجنابي
اسم المشرف: طالب محسن عباس الشافعي
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: A computational study has been carried out to investigate the influence of the optimization variables, by mathematical function represent axial magnetic field distribution for magnetic unsaturated double polepiece magnetic lens. It should be mentioning th

تحضير ودراسة بعض الخصائص الفيزيائية لمادة متراكبة من راتنج الايبوكسي المقوى بالرماد المتطاير وغبار السيليكا == Preparing And Studying Some Physical Properties of Composite Material Which Reinforced With Fly Ash And Silica Fume

اسم المؤلف: محمد باسم غفار الجبوري
اسم المشرف: نهاد عبد الامير صالح المعموري | محمد حمزة المعموري
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تتكون المواد المركبة من دمج مادتين او اكثر مختلفتين في الخواص الميكانيكية والفيزيائية ينتج عن ذلك مادة جديدة ذات خواص هندسية وفيزيائية تختلف عن خواص المواد الداخلة في تركيبها. ان اهم المواد المستخدمة في هذا البحث هو راتنج الايبوكسي مادة كيمائية تعتبر احد | Composite material consists of merging two composite materials or more are different in mechanical and physical properties resulted in a new substance has new different properties differ from the properties of the composing materials. Most important mater

حسابات طريقة دالة الكثافة لانظمة جزيئية واهب جسر قابل == Density Functional Theory Calculations For Donor - Bridge - Acceptor Molecular Systems

اسم المؤلف: جعفر طعمة احمد
اسم المشرف: حامد ابراهيم عبود محمد التميمي
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:

تاثير اضافة (الفضة, السيريوم) على بعض الخصائص الميكانيكية والتركيبية لسبيكة (المنيوم - سليكون) == The Effect of (Ag, Ce) Addition on Some Mechanical And Structural Properties For (Al - Si) Alloy

اسم المؤلف: الحاق عبد مسلم حسن صكبان
اسم المشرف: عبد العزيز عبيد موسى العكيلي | جاسم محمد سلمان المرشدي
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تعد سبائك الالمنيوم المسبوكة مع السليكون كعنصر سبك رئيس من اهم السبائك التجارية بسبب امتلاكها خصائص سبك فائقة, ولهذه السبيكة تطبيقات واسعة في صناعة السيا ا رت والفضاء. يهدف هذا البحث الى دارسة تاثير اضافة عنصري الفضة والسيريوم على سبيكة )المنيوم_سليكون(, | Aluminum alloys with silicon as the major alloying elements are the most important commercial casting alloys because of their superior casting characteristics, which are widely applied in the aerospace and automotive industries.This work aims to study th

تاثير اضافة غشاء الالمنيوم الرقيق على بعض الخصائص الفيزيائية لمتراكب PVA - Ag النانوي == Effect of Addition of Al Thin Film on Some Physical Properties of (PVA - Ag) Nano - Composite

اسم المؤلف: دلال حسن عبد الكاظم الشمري
اسم المشرف: بهاء حسين صالح ربيع المرعب
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تم تحضير متراكبات نانوية (PVA - Ag) وبتراكيز مختلفة باستخدام طريقة الصب Casting method على الغشاء المعدني لمادة الالمنيوم Al) ) المحضربطريقة الترذيذ بالبلازما بسمك 150 nm) ) وتحت ضغط مقداره (2×10 - 1 mbar/pa) وفي نفس الوقت تم تحضير متراكبات نانوية ((PVA - | Nanocomposite (PVA - Ag) were prepared with different concentration by using casting the method of casting on the film material of Aluminum metal, prepared by plasma sputtering with thickness of (150 nm) and under pressure of (2×10 - 1mbar/pa). and has be

دراسة بعض الخصائص الفيزيائية لمتراكبات (PVA - PAAm - CoO) النانوية == Study of Some Physical Properties For (PVA - PAAm - CoO) Nanocomposites

اسم المؤلف: حيدر محمد محسن التميمي
اسم المشرف: مجيد علي حبيب | رحيم كعيد كاظم
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: البحث يتضمن تحضير المت ا ركبات النانوية (PVA - PAAm - CoO) باستخدام طريقة الصب ركيز مختمفة من جسيمات اوكسيد الكوبمت النانوية المضافة. وقد درست بعض الخصائص الفيزيائية قبل الاضافة وبعدها.اظهرت نتائج المجهر الالكحروو الماسح ) SEM ( وجحى ل فىر ر للاشعة جح | The research includes preparation of (PVA - PAAm - CoO) nanocomposites by using casting technique with different concentration of nano additives (cobalt oxide nanoparticles) and study of some physical properties of (PVA - PAAm - CoO) nanocomposites films

تحضير مادة مطاطية مركبة لتصنيع مانعات تسرب الزيوت == Preparation of Rubber Composite Material Used For Manufacture of Oil Seals

اسم المؤلف: سعد عباس جاسم الجبوري
اسم المشرف: نهاد عبد الامير صالح المعموري | محمد حمزة المعموري
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تم في ىذا البحث تحضير عجنة مطاطية مركبة لكي يتم استعماليا في تصنيع مانعات تسرب الزيوت وقد جرت الد ا رسة عمى اربع محاور الاول تضمن اختيار مطاط الاكريمونتريل بيوتادايين ) (NBR كمادة اساس تم في بادئ الامر اضافة مادة اسود الكاربون الييا وبنسب تحميل ( 0,20,30, | Composite materials are used for many application such as science, synthetic and space and space discovery and many application in all life field, because what it has from a special properties for example, strength, light weight, cost …etc,.This work was

دراسة خواص مستويات الطاقة لنظائر 130 - 140,144 - 150Nd باستخدام انموذج البوزونات المتفاعلة الاول (IBM - 1) == A Study of Energy Level of Nuclear Properties of 130 - 140,144 - 150Nd Even - Even Isotopes By The Interacting Boson Model - 1 (IBM - 1)

اسم المؤلف: فاطمة ستار جابر
اسم المشرف: محمد عبد الامير الشريفي
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: In this theses, 130 - 140,144 - 150Nd isotopes have been studies by the interacting boson model (IBM - 1) to determine the energy levels, In addition, by the program IBMT was used for evaluating the reduced electric transition probability B (E2) and the e

دراسة الخواص الكهربائية والبصرية للمتراكبات (PVA - PVAC - Ti) النانوية == Study The Electrical And Optical Properties of (PVA - PVAC - Ti) Nanocomposites

اسم المؤلف: وليد خالد كاظم الشمري
اسم المشرف: مجيد علي حبيب
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: In this study, (PVA - PVAC - Ti) nanocomposites have been prepared by using casting method with different weight percentages of titanium nanoparticles. The (PVA - PVAC - Ti) nanocomposites have been diagnosed by different methods such as fourier transform

The Effect of Coupled - Channels In Collisions Between Heavy Lons Near The Coulomb Barrier

اسم المؤلف: نور هادي عباس
اسم المشرف: khalid S. gassim | fouad A. mageed
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:

تقدير السلينيوم بالطرق الطيفية في نماذج حياتية وبيئية == Estimation of Se By Spectrophotometric Method In Vital And Environmental Samples

اسم المؤلف: داليا عبد الامير احمد
اسم المشرف: صادق جعفر باقر
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: يعد مرض السكري من الامراض المزمنة الواسعة الانتشار في العالم، ومن المشاكل الصحية الرئيسية في معظم دول العالم يضم هذا المرض مجموعة من الاضطرابات الايضية المختلفة (كاربوهيدرات، بروتينات، دهون )، اهمها ارتفاع مزمن في مستوى سكر الدم، حيث ان لتحلل الكلوكوز ف | Diabetes is a chronic disease widespread in the world and lt is amajor health problem. It is agroup of metabolic disorderd including carbohydrates , proteins and lipids. It is considerded as chronic high blood sugar (hyperglycemia). The glycolytic pathway in the cells play an important role to produce the serine which is formed by turning the phosphoglycerate in a series of steps. Serine is behaved as a donor of methyl group catalyzed by serine hydroxyl methyl transferase to produce homocysteine in a series of steps using vitamine B12 and tetrahydro folate (folate cycle) to control the lack of homocysteine that causes high damage to the lining of arteries and other organs , which in turn leads to partial decrease in insulin secretion or lack of insulin receptors. This may effect the eyes , kidneys , blood vessels, and nerves. This research includes people with diabetes type II. admitted to the teaching hospital in Tikrit, Samples have been taken from 40 patients (22 males and 18 females) and (5) samples of healthy males from the staff of the University of Tikrit, The study included two parts : - 1 - Biochemical study : - included the measurement of plasma level of homocysteine from the blood of patients with type II diabetes which showed : - 1 - Significant increase (P

فحص عوامل عنق الرحم باستخدام فحصي اختراق النطف واتصال النطف بمخاط عنق الرحم للازواج العقيمين == Examination The Cervical Factors By Using Sperm Penetration Test And Sperm Cervical Mucus Contact Test In Infertile Couples

اسم المؤلف: الاء عمران مطلك الحسناوي
اسم المشرف: فارس ناجي عبود الهادي | بشرى جابر الربيعي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The present study was conducted in Infertility unit / Babylon Hospital of Gynecology and Children in a period 21/1/2013 to 15/1/2014. The study involved communiqués tests for males and females complaining from unexplained infertility , in which seminal fluid analysis were normospermia. The study include 45 seminal fluid specimens used in connecting test (SCMCT) and penetrating test (SCMPT) with the specimens of cervical mucus of their wives. Cervical mucus aspirating in day 12 - 14 of menstrual cycle. The study aimed to evaluate the husband's sperm activity and concentration in vitro with cervical mucus. Seminal fluid analysis were achieved and then semen specimens used to performed two in vitro tests : SCMCT and SCMPT. sperm parameters ( sperm concentration and sperm motility grade a, b, c, and d) were evaluated in each mentioned test and compared the results with the values of sperm parameters in seminal fluid analysis (control). The statistic results showed 60% of the study specimens of SCMCT was negative and 40% positive, while in percentage of positive results was 33% and 67% negative in penetrating test. The results revealed a significant (p<0.001) decrease of sperm concentration and grade a and b sperm motility, a significant (p < 0.001) increase of sperm motility grade c and immotile sperm grade d in connecting test with cervical mucus compared to control. The results of penetrating test showed significant (P< 0.001) decrease of sperm concentration in the brim, medium and distal region of cervical decrease (p<0.001) of sperm concentration in both medium and distal region compared to brim region, and a significant (p < 0. 001) decrease in distal region compared to medium region ; A significant (P < 0.001) decrease of grade a + b motility percent in all three regions of cervical mucus area compared to control, while no significant differences (P > 0. 05) between brim, medium and distal region of cervical area ; Only grade C sperm motility in distal region showed a significant decrease (P < 0. 001) compared to control and both brim and medium regions. The results revealed significant increase of grade d (P<0.001) in brim and medium region (P < 0.01) compared to control, while there was a significant decrease (P< 0. 001) in distal region compared to control, brim and medium regions. The correlation (r) study of sperm concentration and sperm motility ( grade : a + b, c and d) with age and infertility period in SCMCT showed non significant (P > 0. 05) correlation, except the correlation between a + b grade sperm motility and age it was noticed a significant negative correlation (P < 0. 05) and positive correlation(p> 0.05) between grade c sperm motility and age. The study of linear correlation of sperm parameters in penetrating test showed a significant(P<0.05) negative correlation between grade a+b sperm motility and age in brim and medium region of cervical mucus area. Except this, there are no significant correlation (P>0.05) between sperm concentration and sperm motility for all grades (a + b, c and d) with age and infertility period in both brim and medium regions It was concluded that both test : SCMCT and SCMPT were necessary to diagnosis the causes of infertility when there were normal finding of both seminal fluid analysis and female criteria. mucus area compared to sperm concentration in control, also a significant

التمنيع والتحدي لبكتريا Citrobacter freundii فاقدة الجدار المسببة لخمج السبيل البولي == Immunization And Challenge For Walled Citrobacter Freundii And Walled Defective That Causing Urinary Tract Infection

اسم المؤلف: عبد الرسول عويد عداي الشبيب
اسم المشرف: قاسم نجم ثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: Cryptic bacterial infection has been studied in urinary system to Human, where 50 underwent urine samples from patient mostly based consulting auditors Hilla public Education Hospital for the period from November 2012 until Feb. 2013. Patients ranged in age from 20 - 50 years males and females bacterial infection rate was 70% distributed between the cell wall defective bacteria had 38% and 32% cell - wall bacteria and the results showed 30% as negative cases. Amicroscopic examination of the urine sludge Showed clear sovereignty of non - specialized immune response type of mixed cell 34% followed by mononuclear cell 30%. Samples were cultured on routine media (Blood agar , Macchonky agar) and Modified media (Variant agar, Variant broth). Out put diagnosed by bacterial system diagnostics, phen - otypic, microscopically and biochemistry by using the special test for microorganism diagnosis and using API 20 E and the use VITEK 2 to confirm some samples results. Characterized by colonies of cell wall defective bacteria grow on variant agar it resembles fried egg which could seen after staining Deinse stain. Citrobacter freundii recorded 12%, the highest rate among cellwall defective isolates. Sensitivity test for bacterial isolates were screened from U.T.I both two type (CWB & CWDB) showed the highest rate of sensitive to Impinem 100% represented CWDB, as well as the highest rate of resistance to Ampicillin 100% represented by the same group of isolates. experimental study with urinary tract infection by injecting bacterial stuck of Citrobacter freundii to the three groups of rabbits : first group ( 3 Rabbits ) : immunized with viable surface antigen extracted from CWDB, second group : ( 3 Rabbits ) immunized with heat killed surface antigen extracted from CWB, the third group (4 Rabbits) represented control group it been treated with sterile normal saline. the injection process through urethra, and then underwent a histological study, was histological damage to the members of the animal - derived non - immunized (control group) seemed clear was the appearance of symptoms of the total cellular infiltration and disruption of the mucous layer and epithelial and other symptoms. The immunized animals have shown their ability to defy injury. It is the study of serological reactions to bacterial antigens Citrobacter freundii immunized with vaccines for rabbits given high caliber agglutination with its specialized vaccines and low with adversity and that the absorption and the corresponding and variant antigen does not remove the agglutination effectiveness with immune sera prepared suggesting that the absorption is incomplete and that the difference between the wall and a wall defective in terms of antigenic is quantitative and qualitative.

الاضطرابات الفسلجية والاجهاد التاكسدي لدى النساء في سن بعد الياس في محافظة بابل == Physiological Disturbances And Oxidative Stress In Postmenopausal Women In Babylon Province

اسم المؤلف: سارة حاتم عجيل خضير
اسم المشرف: داخل غاني عمران الوطيفي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: يتميز سن الياس (Menopause) لدى الاناث بتوقف الحيض وتصبح الانثى غير خصبة بسبب فقدان جميع الحويصلات المبيضية (Ovarian follicles) والانخفاض الشديد لهرموني الاستروجين والبروجسترون صممت الدراسة الحالية لفحص الاضطرابات الفسلجية المختلفة والتي تشمل بعض المعايير | Menopause marks the time in women's life when her menstruation stop and she is no longer fertile because of exhaustion of ovarian follicles and sharply decrease in ovarian production of estrogen and progesterone.The present study was designated to determinate different physiological disturbances which occurring in some hematological and biochemical parameters of postmenopausal women. A total number used was 130 women, 100 women were postmenopause and subdivided into four age groups (46 - 50 year, 51 - 55 year, 56 - 60 year, and 61 - 65 year). Thirteen women were premenopause , used as a control group, their ages ranged between 20 - 30 years. In all age groups of postmenopausal women, it have been found that the levels of estradiol hormone were progressioly decreased (p<0.05) when compared with control group. Regarding hematological parameters, RBCs count was insignificantly increase (p>0.05) in all age groups of postmenopausal women. Levels of hemoglobin concentrations and packed cell volume were significantly increased (p<0.05) in all age groups of postmenopausal women compare to control groups. Blood platelets count recorded insignificant fall (p>0.05) in most age groups of postmenopausal women.As for, red blood indices (MCV, MCH, and MCHC) explained insignificant differences (p>0.05) of most menopausal women in a comparison with those control group. Total white blood cells have insignificant elevation (p>0.05) of most groups except 56 - 60 years groups. Regard to differential white blood cells, Levels of lymphocytes and monocyte have been showed insignificant drop (p>0.05) of the level of lymphocytes and significant increase (p<0.05) of first and second age groups in the levels of monocytes of postmenopausal women. Granulocytes white blood cells have been indicated different changes : Nentrophils were significantly higher (p<0.05)in all age groups of postmenopausal women, where as, eosinophils and basophils were insignificantly different (p>0.05) in most age groups. Concerning biochemical changes, Total serum proteins, Albumin, and globulin have been showed significant lowering (p<0.05) in all age groups of postmenopausal women. Statistical analyses showed a significant rise (p<0.05) of serum uric acid and urea levels in most group of postmenopausal women. Measurments of enzyme activities involved in liver (ALT and AST) were significantly hightened (p<0.05) in most age groups of postmenopausal women, Whil, ALP activities recorded insignificant increase (p>0.05) in all age group of postmenopausal women. Determinations of minerals showing a significant increase (p<0.05) in the levels of calcium and insignificant increase (p>0.05) in the levels of phosphorus in all groups of post menopausal women. Analyses of electrolytes have been explained a significant elevation (p<0.05) of the sodium and non significant difference (p>0.05) of the potassium in all groups of postmenopausal women.The present study involved determination specific and essential trace elements, Levels of copper explained a significant decrease (p<0.05) in all age groups associated with a significant increase (p<0.05) of zinc levels in postmenopausal women. Inversly, Values of magnesium were insignificantly different (p>0.05) in all age groups of postmenopausal women. The present study selects specific parameters to explaine oxidative stress in postmenopausal women. Levels of malondeheyds were significantly elevated (p<0.05) in all groups of postmenopausal women, and correlated negatively (r= - 0.102) with level of estrogen. Values of reduced glutathione showed a significant decrease (0.05) in postmenopausal women and correlated positively (r=0.64) with the estrogen hormone. Analyses of antioxidants enzymes have been showed a significant decrease (p<0.05) in the activity of catalase enzymes of all groups of postmenopause. Also, the activity of glutathione peroxidase was significantly decrease (p<0.05) in postmenopausal women and its correlation coefficient was r=0.263. In conclusion, changes summarized above may be attributed to deficiency of estradiol hormone in postmenopausal women which resulting in different physiological disturbances and these changes become aggravated and exacerbated because of oxidative stress occurring in decrease of estradiol hormones.

دراسـة تصنيفية للجـنـس Rhagadiolus Scop. (Compositae) في العراق == A Systematic Study of The Genus Rhagadiolus Scop. (Compositae) In Iraq

اسم المؤلف: اوراس عبد السيد مهدي الحسيني
اسم المشرف: عبد الكريم خضير البيرماني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية دراسة اربعة انواع للجنس Rhagadiolus Scop. في العراق دراسة تصنيفية مقارنة شملت الصفات المظهرية والتشريحية وحبوب اللقاح والبيئة والتوزيع الجغرافي للانواع اضافة الى التصنيف العددي.شملت الدراسة المظهرية صفات الجذور والسيقان والاوراق وا | The genus Rhagadiolus Scop. in Iraq has been systematically studied. Work involving comparative morphology, anatomy, palynology, ecology & geographical distribution and numerical taxonomy has been presented. The morphological study includes studying the characters of root, stems, leaves, inflorescences, phyllaries, flowers, and fruits in addition to indumentums. It has been clear that the characters of stems, leaves, involucres bracts and fruits have a taxonomic importance.Also it has studied some of the micro morphological characters and their taxonomic importances were assessed.The present study showed that the pollen grains of all species were found to be tricolporate with echinate surface & of little taxonomic value. This study also includes characters of the epidermal leaf and stems, indumentums, venation and transverse section of stems and leaves discussion were also included, the taxonomic importance of each character & its variation, it is shown that these characters have an importance assessing morphological characters to isolate these species.Data obtained from herbarium specimens, literature and trips was utilized to elucidate the ecology of the species. Maps of geographical distribution were prepared for all species studied. It has been shown that R.angulosus was the most widely distributed species in the country. The species under current study were numerically treated ; dendrograms and polygonal graphs were drawn for the species of the genus. In conclusion (4) species of the genus Rhagadiolus : R.angulosus, R.edulis, R.hamosus and R.stellatus were recognized for Iraq, taxonomic treatment has been carried out for all Rhagadiolus species and key was designed for them too.

دراسة مناعية لمستضد متعدد السكريد للفطر Aspergillus niger في الارنب == Immunological Study of Aspergillus Niger Polysaccharide Antigen In Rabbit

اسم المؤلف: غدير كاظم عبود الخفاجي
اسم المشرف: نداء شهاب حمد | فريال جميل عبد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: جمعت خلال هذه الدراسة 50 عينة تمثلت ب 25 عينة تربة و25 عينة قشع من اشخاص مصابين بالتدرن الرئوي والوافدين لمركز التدرن والامراض الصدرية في محافظة بابل خلال المدة المحصورة للفترة من تشرين اول 2013 ولغاية شباط 2014 تم خلالها عزل الفطر Aspergillus niger وبنس | fifty specimens were collected, 25 samples were collected from soil while the other 25 sample were collected from sputum of pateints with Tuberculosis, who admitted to thoracic diseases center in Babylon province in period October 2013 to Febreury 2014. Aspergillus niger Has been Isolated from these sample with 56% appearance in soil & 48% in sptum of TB pateints.these isolated were examined micro and macroscopically. purified Fungi was culture on potato dextrose broth, after incubation period polysaccharide antigen of A.niger cell wall was prepared for isolated from soil and TB patients suptum. Molish, protein and glucose test to provide that these antigen are polysaccharide, then immunuization of local rabbit of Orcyctalagus conninculus age 3 - 5 months & 1 - 1.5 Kg for males only (3 animals for each antigen) After the immunization period (one month) some of local and systemic immune response parameters (cellular and humoral) to test animals were studied and compared with control animals which immunized with normal saline. The results of study showed that the polysaccharide antigen isolated from these two strains lead to stimulate systemic humaral immune response which had been studied by using direct agglutination test for appendix immunoglobulin passive haemagglutination test (both were used to determined antibody titer), radialimmunodiffusion test in order to determine level of concentration of immunoglobulins (IgG, IgM) and complement part (C3, C4). where The results of direct agglutination titer of animals immunized with polysaccharide antigen 128 and 1 for control animals and heamagglutination tests titer of animals immunized with polysaccharide antigen serum (2560). These results were higher compared with control group titer (10) and this is also true for immunoglobulin and concentration of IgG, IgM for immunized animals compared to control one. In addition to that The concentration of C3 and C4 had been increased in immunized animals compared to the control. cellular immunological response had been measured by using rabbit Neutrophil Elastase Kit. The eresult show an increase in the level of phagocytosis in animals immunoized with polysaccharide antigen isolated from A.niger cell wall of TB pateints 0.37±0.03 pg/dl while decreased in animals immunoized with polysaccharide of soil A.niger 0.72294±0.25548pg/dl compared with control animals 0.6±0.16386 pg/dl. on the other hand the humaral adaptive immune response had been identified by measuring cytokine IL - 2, IL - 10 in serum rabbit, the level of IL - 2 in rabbit immunoized with polysaccharide antigen soure TB patients and soil is 5.4129±1.08979 and 4.2641±0.62997 pg/dl respectively compared with control animals 3.9327±0.55587 pg/dl. The level of IL - 10 had been dropped for immunized animals with A.niger of TB pateints and from soil 4.8762±1.15104 and 8.2632±4.31933 pg/dl while the control animals having the highest level 10.2576±5.23356 pg/dl among the groups. IgE concentration measured significant difference at p>0.05 in immunized animals of A.niger in both TB pateints and soil 1.1701±0.05811 and 1.0770±0.19355 ml/Iu compared with control where IgE0.8723±0.0849ml/Iu. Miotic index of bone morrow immunized and control animals show mean difference is signification at p>0.05.where the result demonstrate that the polysaccharide antigen fissed the bone marro cells compared to controlled one. In order to identify the viability of antigen in protection. challenge dose test made by injection animals with 108 spore/ml of A.niger conidia which prove their protection in immunized animals while the control animals undergo many morphological and behavioral changes after 5 - 7 days of their injection.I addition that the size of lung and spleen increased when compared with immunized one. The histological culture of lung and spleen of challenge test animals in order to identify the ability and speed of their body to evadicate and prevent infection, display that A.niger coloni appeared for control animals (6 rabbit) while disappeared in culture of immunized one. Skin test also performed to experimental animals which indicate that polysaccharide antigen of A.niger interact with hypersensitivity test of rabbit, where the level of late hypersenetivity increased clearly with propability of p>0.05 with the highest level after 24 hours of beginning test compared to controlled one.

دراسة بيئية وحياتية للمجتمع السمكي في نهر الفرات/ وسط العراق == Ecological and Biological Study of fish Community in Euphrates River / Middle of Iraq

اسم المؤلف: ماهر محمد خضيري
اسم المشرف: ميسون مهدي صالح الطائي | مؤيد جاسم ياس العماري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية في نهر الفرات عند سدة الهندية خلال المدة من تشرين الاول 2012 لغاية ايلول 2013 لغرض دراسة بيئة وحياتية المجتمع السمكي، وتم اختيار موقعين، يقع الموقع الاول في شمال سدة الهندية، ويقع الموقع الثاني في جنوب سدة الهندية، وتضمنت الدراسة تاث | The Present study was carried out on the Euphrates River during the period from October 2012 to September 2013 to study ecology and biology of fish community in the river, two study sites were chosen the first one was in the north and the second one was in the south of Al - Hindiya dam.The study was included the effects of some physical, chemical factors of water and Al - Hindiya dam on ecological and biological of fish population as well as measurement of the concentrations of some heavy metals (Pb, Cd, Mn, Zn) in both dissolved and particulate phases of water and in the tissue of three types of freshwater fish that was high dominance in three age groups. Study of the number of species, individuals, Total of weight of individual. Some ecological indices were study as index of Relative abundance, Species Richness index and Shanon wiener index for determining the evaluate of biodiversity of fish. Also Jaccared index of similarity was used to explain the similarity between sites of study and among months. Constant index was used to determine the constancy of taxa. Biological study of this of three fish that have high dominance from the side of food, age, growth, sex ratio, gonado somatic index and length - weight relationships. Air temperature and water temperature were ranged in two study sites between (15 - 43.8) C? and (10 - 29) C? respectively, pH values were between (7.6 - 8.6), Water current velocity values were between (0.14 - 0.72) m/sec, light penetration values were between (27.3 - 193.3) cm, the value of electrical conductivity were between (822 - 1323) ? sem/cm , Total dissolved solid values were between (663.3 - 932) mg/L, while The total suspended solid were between (0.009 - 0.23) mg/L and the values of dissolved oxygen were ranged between (6.13 - 10.33) mg/L.The biological oxygen demand were recorded values that range between (0.68 - 4.3) mg/L. . The water of Euphrates river were considered slight alkaline according to the results of present study and ranged between (33.33 - 164.3) mg CaCO3/L and the water were very hard according to high values record for total hardness (391.6 - 886.6) mg CaCO3 /L, while the values of calcium and magnesium hardness were ranged between (86.1 - 188.4), (19.8 - 138) mg CaCO3/L respectively.The values of phosphate were ranged between (0.51 - 13.12) ?g/L and Nitrate values were ranged between (6.23 - 70.17) ?g/L, while the values of nitrite were ranged between (0.17 - 4.98) ?g/L. in especially, the effects of Al - Hindiya dam, the results were showed that numbers of species before and after of Al - Hindiya dam were ranged between (23 - 17) species respectively. the results were showed positive and negative correlation between physical, chemical properties of water and Heavy metals with number of species, individuals, weights and Biodiversity indices. This study showed that the mean concentrations of the heavy metals (Pb, Cd, Mn, Zn) of the dissolved phase in water were (2.14, 1.61, 3.10, 7.63) ?g/L respectively and in the particulate phase were (33.99 112.87، 84.21، 21.12،) ?g/g dry weight respectively, the results of present study were showed that the concentration of heavy metals in water in dissolved and particulate phases within local and world limitations. The heavy metals in muscular tissue for three groups of this fish were ordered in as following : Zn >Mn > Pb > Cd. Twenty - three fish species were collected, (17) of them belonged to Cyprinidae family and two species belonged Bagridae family while only one species represented each family of Mugilidae, Siluridae, Mastacembelidae, Cichlidae, the number of species were ranged during the period of study between (5 - 18), The total number of individuals was 2401 individual and the number of individual were ranged monthly between (129 - 276) individual, while the total weight of individuals were 232868 gm during the period of study and The weight of individuals were ranged monthly between (13072 - 29598) gm. Carassius auratus wae recorded with the highest percentage in number and weight as 35.2% and 27.5% respectively. while A.vorax was after C.auratus in total number 13.6%, but it was after C.auratus and C.carpio in total weight 16.9% of the total individual weight. The values of species richness index were ranged between (0.79 - 3.23) and numerical of shanon wiener index values (Hn) were ranged between (1.26 - 2.39), while the weight of shanon wiener index value (Hb) were ranged between (1.21 - 2.35). the numerical and weight of eveness index (Jn) were ranged between (0.65 - 0.86), (0.68 - 0.89) respectively during the period of study. Jaccared similarity index were ranged between (23% - 92%). And the study was revealed that the most species recorded during the period of study were constant species according to constancy index. C. carpio fish were Omniovorous and It's feeding activity was ranged between (70% - 91.42%) and feeding intensity was (6.9 - 12.17) degree/fish.while C. auratus were detritious, It's feeding activity was ranged between (56.75% - 94.28%) and feeding intensity was (6.91 - 12.87) degree/fish. A. vorax were carnivorous and It's feeding activity values were ranged between (69.2% - 90.32%), while feeding intensity values were ranged between (6.85 - 13.03) degree/fish. The results was showed the values of age and length for C. carpio that ranged between (1 - 5) years, (9.7 - 40.2) cm , and C. auratus were ranged between (1 - 6) years, (8.3 - 28) cm, while A.vorax were ranged between (1 - 7) years, (10 - 62) cm respectively. Sex ratio and gonado - somatic index (GSI) varied between the three species, sex ratio of C.carpio (male : female) was 1 : 3.1. (GSI) for male was ranged between (2.21 - 12.02) and for female between (3.36 - 19.36).The sex ratio of C. auratus male : female was 1 : 1.9, GSI values for male was ranged between (0.89 - 8.53) and for females between (1.23 - 61.37). A.vorax sex ratio male : female 1 : 1.5, GSI value for male and female were ranged between (0.36 - 2.84) and (0.24 - 5.83) respectively.Variation of values of slope factor (b) were calculated from length - weight relationships for three species of fish, the values of (b) factor for C.carpio, C.auratus and A. vorax (3.08, 2.97, 2.92) respectively during the period of study

دراسة جزيئية، وخلوية وراثية، ومناعية لمرض سرطان القولون والمستقيم في العراق == Molecular, Cytogenetic And Immunological Study of Colorectal Cancer In Iraqi Patients

اسم المؤلف: حيدر جاسم محمد الخفاجي
اسم المشرف: علي حمود السعدي | عزام قنبر اغا
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The study consists of three parts related with patients of CRC as in : 1 - Molecular part 2 - Cytogenetic and molecular genetics part and 3 - Immunological part. In addition of their relatives of clinical and pathological diagnosis, genus, and tumor locations in 150 of Iraqi patients confirmed with CRC collected from Teaching Hospitals of some Iraqi governorates. The percent of male to female (54.66% / 45.43%) and the aged range of study were 16 to 80 years.The first part consist a study of five types of genes related with CRC represented by MLH1, MSH2, APC, K ras, and SMAD7 genes. Some of these genes develop with germline mutations as in MLH 1 exons1 and 15 so as MSH2 genes. While the rest genes related with a sporadic of CRC. All these genes were amplified by conventional polymerase chain reaction (PCR) for study the types of the mutations and their defect of these genes on CRC. The APC exon11and K ras exon1 genes were processed by single strand conformational polymorphism (SSCP) technique. The results of PCR - SSCP technique for two genes represented by appeared three types of bands, which were (AA), (AB) and (AC) bands. The important band was (AB) which constitutes the variability region represented by 84.2% and 44.82% in patients for both genes. The results of patients with mutated bands with high significant differences(P?0.05).compared with healthy control. Patients carried these bands subjected to PCR - Sequences technique. The analysis results for APC gene sequences were substitutions mutations type of single nucleotide polymorphism (SNP) change Cytocine to Thiamin (C>T) at 1972 location of genome. While the analysis results of Kras gene appeared two types of mutations, substitution and frame shifts represented by deletion mutation and so as appeared stop codon mutation.The other genes represented by MLH1, MSH2, and SMAD detected by direct PCR - Sequences. The results analysis of MLH1 gene, exon (1and 15) represented by nucleotides change with high frequencies of substitution mutation through exon1 at different locations of nucleotides. So as frame shift mutation type deletion through exon15. While for MSH2 gene, exon 6 from 6.66% represent by deletion mutation. A spread study for SMAD7 gene, exon4 were done through 30 patients average age 52 years (male to female 56.7% : 53.3%) confirmed colorectal adenocarcinoma compared with 15 healthy. A mutation of 20% of (SNPs) were identified.The second part which represented standard conventional cytogenetic and molecular genetics were done through peripheral blood culture (PBC). The study showed increasing of mitotic index (MI) in patients with significant differences (P?0.05) compared with healthy controls indicating for increasing of proliferation of the lymphocytes division. The study showed some chromosomal aberrations with significant, the majority of aberrations were higher significant in dwarf and aneuploidy chromosomes so as elongation chromosomes revealed significant differences (P?0.05) between patients and healthy. The appeared of Polyploidy chromosomes, with significant differences (P?0.05), that’s seem to be good indicator for cancer disease. The molecular genetics alterations which dependent on cytogenetic investigation were done through deletion of long arm of 5 chromosome which APC gene is located. The test elucidates the importance of inactivation (deletion) of APC gene in elicited of colorectal cancer. The study of 20 patients, were investigated by fluorescent in situ hybridization (FISH). The results showed 60% patients which have 5q arm deletion of (+). And (5%) have two deletions (++) which was a male aged less than 60 years. The results analysis explained significant differences (p?0.01) between studied group and deletion (+) of arm 5q. A correlation represented by significant differences (p?0.01)through dysfunction of APC gene deletion of 5q were found with high percent among sporadic adenocarcinoma 58.3%, with less in attenuated familial adenomatous polyposis (AFAP) and familial adenomatous polyposis (FAP) represented by the (25%), and (16.7) respectively.The last part related with immunological test were done by detection of tumor markers represented by Carcino Embryonic Antigen (CEA), IL - 33 and IL - 31.These markers represented a confirmative indicators for colorectal adenocarcinoma which were tested by ELISA technique in (79) patients confirmed with CRC of male and female. These tumor markers in tested with patients showed high significant differences (p?0.01) compared with healthy controls. Another immunological test for detection of Human papilloma virus (HPV) type 18 E6 gene expression in CRC by Immune histochemistry technique (IHC) in 71 (FFPET) of CRC. The results of this study showed there were a correlations between patients and adenocarcinoma positive HPV18 E6 infection 43.7% of patients infected with virus, with high significant differences (p?0.01) and higher infection in female (67.7%) than male (32.3%). So as a correlations were found between HPV18 E6 expression and tumor tissue locations, explained by high percentage 45.2% in rectum.While a tumor marker was (CD8+) was used to determine its role in human immune surveillance in tumor regions of CRC so as with regions which were infected by HPV. The study revealed high significant differences between healthy control and studied groups which have (CD8+) positive infiltration in tumor origins through detection by (IHC).In conclusions diagnosis of CRC can be detected by special genes like K ras gene through professional PCR processing, compact with tumor markers, so as Fluorescent in situ hybridization (FISH) technique provides a precise method which can be used for detection of alteration of molecular and cytogenetic related with CRC.

تاثير الماء المعالج مغناطيسيا في بعض الصفات المظهرية والكيموحيوية في عقل نبات الماش (Phaseolus aureus Roxb.) == Effect of Magnetically Treated Water On Some Morphological And Biochemical Properties of Mung Bean (Phaseolus Aureus Roxb.) Cuttings

اسم المؤلف: عامر راضي عبد الحسين جبار
اسم المشرف: بشير عبد الحمزة محمد العلواني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: This study was conducted in order to investigate the role of magnetically water in the rooting of fresh and aged (phaseolus aureus roxb.) cuttings, and the impact of treated water in some morphological criteria and biochemical that interfere in the induction of rooting in cuttings and fall clearly when delayed induced auxin treatment in aged cuttings. Study was conducted in the laboratories of the Department of biology at the University of Babylon, for the period 2012 - 2014 was the use of magnetic therapy devices (Magnitron) of water with three gauss 1000, 2000.3000 so as to provide a magnetic flow to distilled water and river water and used as well as the complete randomized design and analyzed the results statistically significant and valuable knowledge LSD to extract the significantly differences between the treatments and the results were as follows : Physiological parts1. Increase the number of roots significantly in cuttings when treat for 24 h.with auxin dissolved with distilled water processor in 1000 gauss amounted to 77.75 root / cutting.2. The results showed that the roots rate increased significantly in the treatment of fresh cuttings treated in distilled water processor 1000 gauss gave 64 root / cutting. As well as the rest treatments affect in rooting increase as given (58, 52.25) root / cutting for each of the treatment 2000.3000 gauss, respectively.3. The treatment of river water as well as magnetically influenced in increasing the number of roots significantly in three processes and the highest being in the 1000 gauss(84) root / cutting.4. The results showed that distilled water processor magnetic effect is significant in the processes that occur during ageing of the cuttings, which led to a reduction in rooting rate in aged cuttings, as it was observed that the treatment of the cuttings for 72 - hour in 1000 gauss is the best of the rest of the treatments amounted to 48 root / cutting. As the impact of river water processor magnetically significantly increase the number of roots in the aged cuttings as treatment given to 1000 gauss higher values 70 root / cuting.Biochemical parts.1. The results showed that chlorophyll content increased significantly when using the river water wizard three intensities (18.09, 17.43, 16.99) (spad) intensities of 1000, 2000.3000 gauss respectively.other each of distilled water and river water processor magnetically in 1000 gauss increase in chlorophyll content to 16.07, 18.57 (spad) respectively in aged cuttings.2. It was observed that the treatment of auxin dissolved in three intensities impacted significantly on the effectiveness of antioxidants defense system and the highest being at the treatment of the cuttings in auxin dissolved water magnetically processor in 1000 gauss, as was the effectiveness of the enzyme SOD 1.85 unit in the effectiveness of the enzyme CAT reached 15.30 unit and increased GSH content to 0.914 mg / g when compared with the control treatment.3. influenced the treatment of fresh cuttings in distilled water magnetically processor with three intensities are equally matched in the effectiveness of SOD and CAT, and the highest being at 1000 gauss magnetic intensity as it affected the effectiveness of the CAT to 31.62 units. Magnetic intensity also affected the same increase in GSH content reached significantly to 0.710 mg / g.4. The results showed the treatment of fresh cuttings river water magnetically wizard in three intensities increase in the effectiveness of the enzyme SOD and CAT, and the highest being at the treatment of 1000 gauss, amounting to 4.168, 45.9 U for both enzymes, respectively, as GSH content increased significantly, reaching 0.810 mg / g in the treatment of the fresh cuttings in 1000 gauss.5. I have not noticed any significant effect of distilled water processor magnetically as well as river water in the MDA content.6. The impact of distilled water processor 1000 gauss significantly in increasing the effectiveness of the enzyme SOD and CAT in aged cuttings, amounting to 2.88, 42.84 units respectively, as GSH content increased to 0.785 mg / g when the treatment of tresh cuttings in distilled water processor with 1000 gauss and also affected the rest of the intensities increase in GSH content in aged cuttings.7. increased the effectiveness of enzyme SOD and CAT when treated of aged cuttings in river water processor magnetically 1000 gauss, amounting to 2.9, 51.2 units respectively, as GSHwas significant increase in content to 0.854 mg / g when treatment aged cuttings in river water processor 1000 gauss and also affected the rest of the intensities increase in GSH content.8. The use of treated water magnetically 1000 gauss significantly effect in reducing the MDA content in aged cuttings 0.293 mg/g fresh weight.

دراسة وراثية وبكتريولوجية على بكتريا تثبيت النيتروجين التكافلية Ensifer meliloti == Bacteriological And Genetic Study On Symbiotic Nitrogen Fixing Bacteria Ensifer Meliloti

اسم المؤلف: محمد زهير ناجي المرعب
اسم المشرف: حسن فاضل ناجي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: عزلت عشر عزلات من بكتريا Ensifer meliloti من العقد الجذرية لنبات الجت Medicago sativa L. بعد ان جمعت من مناطق مختلفة من محافظة بابل خلال المدة من شهر تشرين الثاني/2012 لغاية شهر شباط/2013. شخصت العزلات جزيئيا بتقنية تفاعل البلمرة السلسلي فضلا عن طرق ال | Ten isolates of Ensifer meliloti were isolated from root nodules of Medicago sativa L. collected from different areas of Babylon province during a period from November - 2012 to February - 2013. The isolates were molecular identified using polymerase chain reaction technique, in addition to traditional methods (morphological and biochemical). All isolates were harboured nodbox and mucR genes and produced cyclic ? - (1, 2) glucans, cellulose fibers and lipopolysaccharide.The ability of isolates to grow at different degrees of pH, temperatures and different concentrations of sodium chloride were tested. It was found that the optimum pH for growing of Em1, Em3, Em4, Em6 and Em8 isolates was pH7, while for the isolates Em2, Em5, Em7, Em9 and Em10 was pH8. The optimum temperature for growing of Em1, Em3, Em5, Em7, Em8 and Em9 isolates was 30?C, while the optimal growth of Em2, Em 4 and Em6 isolates was 35?C, Em10 isolate showed significantly extremism to grow at temperture reached to 45?C. The optimal percentages of NaCl for growing of Em1, Em3, Em4, Em5, Em6, Em8 and Em9 isolates were 2%, for Em7 isolate was 3% and for Em2 and Em10 isolates were 4%. The effect of different degrees of pH on symbiotic properties was studied. Em1 and Em8 isolates that grew at pH6.5 ; all isolates that grew at pH 7, pH 7.5 and pH 8 were able to infect the root hairs of alfalfa plants. The isolates that grew at pH6.5 (except Em1 and Em8 isolates) and all isolates that grew at pH8.5 were failed to infect the root hairs and did not formed nodules and resembled to the control plants (without inoculum).Different concentrations of NaCl on symbiosis was studied, it was found that all isolates that grew at 2% and Em2 and Em10 isolates that grew at 3% formed nodule, while Em1, Em3, Em4, Em5, Em6, Em7, Em8 and Em9 isolates that grew at 3% and all isolates that grew at 4%, 5% and 6% did not elicited nodules on the roots of alfalfa plant. Light microscopic observations of root hairs inoculated with Ensifer meliloti isolates showed the root hair deformations like curling, waviness, swelling and formation of shepherd`s crook and infection thread. These consequent changes lead to emerge the mature nodules having five distinct zones viz., distal meristematic, infection, inter, nitrogen - fixing and proximal senescence zones.The gel electrophoresis results of plasmid DNA of isolates showed that these isolates harboured two megaplasmids. When the isolates exposed to extreme factors like pH, temperture and NaCl, it was found that the isolates lost these plasmids and its ability to elicite the nodules and failed to fix the nitrogen. This study showed that the nitrogen fixing bacteria had essential role to support the plant growth and increase their productivity, and recommended to use the isolate Em10 as example for biofertilizers and the best alternative to the chemical fertilizers

دراسة انتشار الحيوانات الابتدائية الرئيسية المسببة للاسهال بين المرضى باستخدام الطرائق المجهرية والجزيئية في محافظة بابل == Prevalence Study For Main Protozoa Diarrheal Agents Among Patients By Using Microscopically And Molecular Methods In Babylon Province

اسم المؤلف: ميس كاظم عليوي
اسم المشرف: احمد خضير عبيس الحميري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية خلال المدة من تشرين الاول 2014 الى شهر شباط 2015 حيث تضمنت هذه الدراسة فحص 987 عينة براز (اطفال وبالغين, ذكورو اناث) بطريقة الفحص المجهري المباشر و96 عينة موجبة فحصت عن طريق تقنية تفاعل سلسلة متعدد البلمرة. للمرضى المصابين بالاسهال و| The current study during period was conducted from October 2014 till February 2015 examination of 987 stool samples for direct smear method (Lugol's Iodine, Normal Saline (0.9%) for detection of the following parasites G.lamblia, E.histolytica while using floatation methods and Ziehl - Neelsen method (Malachite green) for detecting the Cryptosporidium spp. by using light microscope) and 96 positive samples from these samples examined by polymerase chain reaction technique PCR. For patients infected with diarrhea (children and adults, male and female) who attended to Babylon maternity and children hospital and specialized Marjan Hospital for Internal and Cardiac Diseases in the Babylon province as well as primary health care and private clinics. The age ranges from(Less than one year - 31and more).The current study showed the rate of infection with parasites that causative of diarrhea47.3% (E.histolytica, G.lamblia and Cryptosporidium spp.) was 26.4%, 17.9% and 3.7%, respectively.They were examined by a direct smear method to detect the trophozoites, cyst and oocyst phases of these parasites. The highest rate of infection by microscopic examination was in the rural area 67.2% in comparing with city that was 32.9%. Also the highest rate of infection among males was 51.5% in comparing with females 41.2%. It has been recorded that the higher rate of infection was 76.1% in the age group (16 - 20) years while the lower infection rate was in the age group (26 - 30) years that was 22.8%. It has been observed significant differences in infection rates at the (P ? 0.05). The present study recorded difference in the rates of parasitic infections according to the presence of animals in houses, the high rates of infection where with those have animals in their houses 51.2% while the lowest rates of infection where with those not have animals in their houses 38.9%.It has been observed significant differences in infection rates at the (P ? 0.05). This study reveals a difference in parasitic infections rates, according to the level of education the head of household, and found that the highest rate of infection for those with the head of the family is non - educated or illiterate 48.3%, while the rate of infection was declined with head of the family who get primary education level 46.2%. It had recorded the highest infection rate in October 63.8%, while the lowest percentage was in February 27. 6%. It has been observed significant differences in infection rates at the (P ? 0.05). In the present study Polymerase chain reaction (PCR) is used to 96 positive results in direct smear methods to detection the main parasitic diarrhea agent. It had recorded total of infection rate of 43.4% (31.3%, 28.1% and 2.1%, respectively). Depending on PCR technique showed the highest rates of infection was in the male 36.7% while the lowest rates of infection in females 30.6%. It had recorded the highest rate of infection in the rural areas 45.3% in comparing rate of infection in the urban areas 25.9%. The present study recorded the highest rates of infection were in the (16 - 20years) age group 46.2%, while the decline in the rates of infection was in the (21 - 25years) age group 16.7%. Also it showed the highest rates of infection were in the presence of animals in the houses 36.1% while the lowest rate of infection where there were no animals 31.4%.It has been observed significant differences in infection rates at the (P ? 0.05). The study found that the highest rates of infection for those with the head of the family is non - educated or illiterate 38.9%, while the rate of infection declined with head of the family who got Academic education level 33.3%. Also it had recorded the highest rate of infection in November (2014) 42.1%, while the lowest rates percentage was in December (2014) 23.1%. It has been observed significant differences in infection rates at the (P ? 0.05). Through the current study it was concluded that the prevalence of the parasites that cause diarrhea in the Babylon province are very highly when detection microscopic examination and PCR method, comparison with previous studies and rural area highest rates of infection from urban area.

التوصيف المظهري والجزيئي لبعض الفطريات الخيطيه والخمائر المعزولة من اخماج العين واختبار حساسيتها الدوائيه تجاه بعض المضادات الفطرية == Morphological And Molecular Identification of Some Filamentous Fungi And Yeast Isolated From Eye Infections And Theirs Susceptibilities Toward Some Antifungals

اسم المؤلف: يسر فاضل عبد الامير الاسدي
اسم المشرف: زيدان خليف عمران المعموري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تمت هذه الدراسة في مختبر التقنيات الاحيائية التابع لكلية علوم البنات / جامعة بابل للفترة من اكتوبر 2012 الى اكتوبر 2013، حيث تم جمع 165 عينة سريرية من المرضى المراجعين لاستشارية العيون في مستشفى الحسين العام ومن بعض العيادات التخصصية لاطباء العيون في محاف | The study was performed in Biotechnical Laboratory in Science collage for women, Babylon University from Oct.2012 to Oct.2013. 165 clinical samples were collected from patients with eyes infections were admitted to AL - Hussein main hospital in Karbala province and private clinics of eye physician, after diagnosis of eye infection by specialize physician the clinical samples collected by using swab from Cornea, Conjunctiva, Lacrimal sac, eye allergic, contact lenses and from pull water eye after surgery, the samples included different sex & age groups.All clinical samples culturing on SDA and PDA, Yeast was identified base on their color reactions on Chromo agar Other biochemical test were performed.Antifungal sensitivity was performed against 8 antifungal (Nystatin, Econazol, Amphotericine B, Flucosytosine, Miconazole Metronidazole, Griseofulvin, Ketoconazol, Most fungi showed variable degrees in their susceptibilities to the antifungal agents. In this study we diagnostic (57) isolate from filamentous fungal species, Aspergillus spp. and Fusarium spp. showed highly frequent in ratio 20.1% and 9.6, In addition (37) yeast isolated, C.albicans and C.famata representative the highest frequent isolates.Molecular identification and genotyping, 8 isolates identified as C.albicans from 24 of selective Candida isolates were performed by specific pair primer (CABC) that give genotype with 515 base pair, genotype ITS1/ITS4 region show polymorphic PCR product help in diagnosis other (7) Candida species like C.famata, C.rugosa, C.glabrat, C.utili, C.saitoana, C.guilliermondii, C.inconpicua. The pair primer for amplified the transposable intron (CA - INT) was succeed in amplification of intron showed two genotypes : type A (450bp) and type B(850bp) and showed there was no C.dubliniensis isolates in our Candida isolate, This result was coincidence typing results between specific pair primer (CABC) and Microsatellite CAI for C.albicans.The amplified 18s region by using NS/EF3 and the result give genotype with molecular weight 1700bp.Also the study include using RFLP technique & the digested by using CfoI enzyme & the results for ITS PCR products of 24 isolates of Candida showed unique RFLP patterns.
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