اسم المؤلف:
حسن كاظم علي الكناني
اسم المشرف:
علي خضير جابر الركابي | علاء جبار عبد ال منهل
المستخلص:
Prepared types of Chitosan designated as (A,B,C,D) that prepared from chitin the extracted from shrimp shells Penaeus semisulcatus by treatment chemical different. Different this types the physiochemical characteristics, using in Preparation edible film form simple and Composite with whey proteins. Properties edible film physical and chemical barrier and were studied, there use it in extending Iraqi Soft Cheese shelf life .The results showed studying contain chemical of shrimp shells high parentage moisture and protein and ash 65.77% and 34.25% and 37.48% respectively while parentage chitin 20.11% and fat 8.16%.Analyze composition chemical the chitin of parentage moisture 5.8% and protein 3.2% and ash 3.3% and fat 2%.Give chitosan A high the yield 15.01% while decrease for types other B,C,D 14.23,13.3,11.18% respectively. Composition chemical the types of chitosan A,B,C,D , high parentage moisture and ash and fat for chitosan A comparing types other(11.6%,1.75%,0.95%) respectively and were types B(9.28%,1.15%,0.76%) and C(7.55%,0.78%,0.54%) and D(6.13%,0.43%,0.36).Showed of chitosan D high parentage Degree of Deacetylation 85.16% while (62.51,75.82,80.12)% for chitosan B,C,D respectively. Molecular weight decrease for chitosan D 897 kDa and increase for types chitosan other was high for chitosan A 1753 kDa. It was observed that a relationship is existed between physiochemical and functional characteristics for each type of chitosan.The nitrogen content , solubility, water and fat binding capacity increased with higher rate of Deacetylation Offset by a decrease in molecular weight and viscosity. Showed chitosan D high rate nitrogen 7.22% and solubility 95.16% some shown high value for water binding capacity 700% was lower for chitosan A 581% some give chitosan D high for fat binding capacity compared by types other was higher with ricinus oil 665% andgive rate different with almond oil and flower sun oil and Olive oil(621,551,526)% respectively. Decrease viscosity for chitosan D 62.84 centipoise while was (78.99,88.45,98.73) centipoises for chitosan A,B,C respectively. Showed of chitosan activity inhibitory for bacteria gram negative higher positive was rate inhibitory for bacteria E.coli وsp.Pseudomonas و%100 and %88 and %100 and %100 at concentrations 1250 and 2500 um/m respectively while rate inhibitory for bacteria gram positive Staphylococcus aureus وcereus Bacillus وBacillus subtilis %95.51 and %100 and %79.21 and %78.7 and %91.54 and %9.53 at concentrations 1250 and 2500 um/m respectively. Show the results cycle chitosan in inhibitory growth mulds of increase rate inhibitory with increase concentration was at concentration 2500 um/m 67.71% and 51.89% for muld Penicillium .sp and Aspergillus niger respectively. Shown higher activity antioxidation at concentration10 um/m from chitosan 72.85% compared for activity antioxidation industrial 78.89%. Preparation films simple from chitosan type B,C,D while eliminating treatment A characterized with self - standing, transparent, tasteless and odorless, clear, homogenous, bendable, glossy and flexible. Increase film thickness chitosan B compared by chitosan D,C 0.044,0.046, 0.048 mm respectively at concentration 1% and glycerol by rate 30%. Shown higher value for tensile strength for chitosan B reach 46.55 Mega Pascal lower was for chiosan D reach 34.19 Mega Pascal and decrease tensile strength with increase concentration the glycerol. Give chitosan D higher percentage elongation at break 22.44% lower was for chitosan B reach in 18.71% increase percentage elongation at break with increase concentration the glycerol .Range rate Solubility of simple chitosan film in water between higher Solubility for treatment D 17.24% and lower for treatment B 10.51% and increase Solubility with increase concentration the glycerol. Range Water vapour permeability between 10..32 - 13.59 gm· mm/m2 h .k Pascal. Composite films It is easy to be removed from the mold. Thefilm were fine texture and the transparent of the films was lowered and it has taken the color of whey protein. increasing tensile strength composite films with whey protein with increased whey protein concentration from 0.5 - 1% with reached 22.34 - 26.73 Mega Pascal while the decrease percentage elongation of composite films with increased whey protein concentration from 0.5 - 2% reach 21.45 - 15.31%. Range The percentage of water solubility was for composite films 21.15 - 36.37% and increase Water vapour permeability for Composite films with increased whey protein concentration from 14.71 - 26.65 gm.mm m²/ h. k Pascal . Shown results Coating of Soft cheese by films chitosan the simple(T3) and compound (T4) activity in percentage lost moisture decreased during time storage14 day on heat 5 - 7C excellent treatment (T3) in treatment (T4) percentage lost moisture decreased 36.50% and 32.03% respectively . Increase rate protein in Soft cheese and all treatments during storage 19.83 and 19.69 and 19.28 and 19.71% for treatments T1,T2,T3,T4 respectively. Decrease in PH during time storage 5.45,5.50,5.65,5.60 for treatments T1,T2,T3,T4 respectively. Get increase in rate in soluble nitrogen to total nitrogen for treatments T1,T2,T3,T4 reached 4.97,4.94,3.02,3.14% after weeks from storage. Decreased of peroxide value by cheese coated by films chitosan simple and coated compared cheeses uncoated. Total bacterial counts were generally higher in uncoated cheese in comparison with cheese samples coated with chitosan. Bacterial total numbers in treated samples of Soft cheese were decreased with an approximate average of 1 - 2 log CFU/g in comparison with uncoated Soft cheese. And decreased average of Psychrophilic , proteolytic ,lipolytic , coliform bacteria in coated cheese of all treatments in comparison with uncoated Soft cheese and too molds were decreased in average numbers coated cheese of all treatments in comparison with uncoated Soft cheese. .The results of examination revealed no Staphylococcus aureus growth of this group. The results showed the sensoryevolution of samples : coated and non - coated, the high value was observed in pieces cheeses coated , because it get the high evaluation sensory from where (color , outside appearance, body and texture, taste and flavor) degree from the judges as compared with non - coated pieces.
