تاثير مصدر الجزء النباتي وتركيز منظمات النمو في اكثار نبات الداليا الهجينة Dahlia variabilis Wild. (Desf.) خارج الجسم الحي == EFFECT OF SOURCE OF EXPLANT AND GROWTH REGULATORS ON IN VITRO PROPOGATION OF DAHLIA PLANT (Dahlia variabilis Wild. (Desf.))
Author name:
اسراء عبد المحسن دراج عباس
Supervisor name:
ماجد عبد الحميد ابراهيم
General topic:
Agricultural sciences
Specific topic:
Plant - Gardening
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture - Department Of Horticulture And Garden Engineering
Language:
Arabic
University location:
Basrah
First pages:
31T1758 - p.pdf
Abstract:
The current study was conducted in the Laboratory of Plant Tissue Culture, College of Agriculture, University of Basrah during the period of 2013 - 2015. The aim of this study was micro propagation of dahlia plant (Dahlia variabilis Wild. (Desf.)), using several methods of In vitro culture. This study was conducted several experiments involving test source of explants and the effect of growth regulators in various stages of propagation, and the results of the study are as follows : - 1. The germination percentage of seeds that cultured on MS medium supplemented with 2+0.3 mg.l - 1 combination BA and NAA respectively was 100% , while the seeds did not germinate when they cultured on MS medium provided of concentration of 0+0, 1+0 and 1+0.3 mg.l - 1 each of BA and NAA.2. The germination percent of seeds cultured on MS medium supplemented with 2+0.3 mg.l - 1 BA and NAA that grown in darkness reached 100% . but these seeds did not germinated when they grown in the light.3. Shoot tip was superior significantly on the epicotyl in the percentage of shoot multiplication, which reached in each 86.67% and 43.33% respectively. While cotyledonary leaves, nodal and root segments did not show any response to shoot multiplication.4. The shoot tip cultured on MS medium supplemented with 2.0+2.0 combination of BA and NAA gave the highest rates for the number of shoots, shoot length, number of leaves and leaf area, which reached 5.00 shoots, 3.33 cm, 5.33 leaves and 0.40 cm2, respectively. This combination was superior significantly on other combinations of BA and NAA (1.5+1.5, 2.5+2.5 and 3.0+3.0 mg.l - 1).5. The results showed that the shoot base segment formed direct adventitious shoots when cultured on MS medium supplemented with 2.0+2.0, 2.5+2.5 and 3.0+3.0 mg.l - 1 combinations of BA and NAA. But, that shoot base segment cultured on MS medium supplemented with 1.5+1.5 mg.l - 1 combination of BA and NAA showed no response to the formation of shoots after eight weeks of culture. Also, theresults showed that the shoot segments cultured on MS medium supplemented with 2.0+2.0 mg.l - 1 combination of BA and NAA has superior significantly rules on other combinations of BA and NAA in the number and length of shoots (10.00 shoots and 6.67 cm, respectively).6. Shoot tips and nodal segments cultured on MS medium supplemented with 3.0 +3.0 mg.l - 1 combination of BA and NAA were superior significantly on epicotyl in the percentage of callus induction, which reached 75.00%, 60.67% and 23.33%, respectively, after eight weeks of culture. The other explants (cotyledonary leaves and root segments) cultured on the same components of the medium, they did not respond to the induction of callus.7. The shoot tips were growing on MS medium supplemented with 3.0+3.0 mg.l - 1 combination of BA and NAA gave a large amount of callus. While these shoot tips cultured on MS medium supplied with 1.5+1.5 mg.l - 1 did not give any response to the induction of callus.8. The results showed indirect organogenesis from callus developing from the shoot tip and epicotyl on MS medium added with 3.0+3.0 mg.l - 1 combination of BA and NAA while cotyledonary leaves, nodal and root segments did not respond to organogenesis when cultured on the same medium components.9. The two combinations of BA and NAA (2.0+2.0 and 2.5+2.5 mg.l - 1) were superior significantly on the 3.0+3.0 mg.l - 1 combination in the number and length of shoots producing from the developing callus of shoot tip, which reached : (6.3 and 5.67) shoots and (6. 67 and 5.67) cm, respectively. While the shoot tips did not respond to organogenesis when cultured on MS medium added to 1.5+1.5 mg.l - 1 combination of BA and NAA.10. The shoots cultured on half strength of MS medium supplemented with 1.5 mg.l - 1 NAA were superior significantly on other concentrations (0.5 and 1.0 mg.l - 1), in the percentage of rooting, the number of secondary roots and root length which reached 81.67%, 10.33 root and 1.47 cm respectively .11. The adventitious shoots cultured on half strength of MS medium added to 0.6 mg.l - 1 IBA and 45 gm.l - 1 sucrose gave the highest percentage of rooting and the highest rates of the number of primary and secondary roots and root length.12. Dahlia plants were acclimatized a high success rate of 100% when cultured in plastic pods containing a mixture of fine sand and peat - moss sterilized by 2 : 1.
