تقييم التنوع الوراثي في تجمعات الابل العراقية باستخدام تقنية التتابعات الدقيقة == ASSESSMENT OF GENETIC DIVERSITY IN IRAQI CAMELS POPULATION USING MICROSATELLITES TECHNIQUE
Author name:
ايوب راضي طعمة زعلان
Supervisor name:
طالب احمد جايد
General topic:
Agricultural sciences
Specific topic:
Zoology
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1761 - p.pdf
Abstract:
This study was conducted in the Genetic Engineering Lab., College of Agriculture, University of Basrah (UoB). Iraqi camels divided into six provinces namely Basrah, Maysan, Dhi Qar, Qadisiyah, Muthannah and Najaf, aimed to measure the genetic diversity, genetic polymorphism for Iraqi camels, measure the ratio and the amount of heterozygosity in addition to the study of the genetic relationship between these populations. The blood samples were collected from six unrelated Iraqi camels populations by 100 samples by 5 ml from the jugular vein using a medical syringe. The blood was stored in test tubes containing anticoagulant agent like EDTA. Samples were numbered to know the area where the samples were taken. DNA was extracted using Kit from Invitrogen company, U.S.A according to the instructions attached with kit with some necessary modifications. The amount of DNA per sample measured by using Nano drop technique (Thermo scientific company, USA).Twelve microsatellite markers for camels (CMS50, CMS121, CMS13, VOLP67, LCA66, CVRL06, CVRL05, CVRL01, VOLP32, VOLP03, WYLL44, WYLL08) were used in this study. Polymerase chain reaction PCR was done for these microsatellite markers then fragmentations DNA analysis was conducted. Results were tabulated and statistically analyzed using special programs for population genetics such as popgene. can summarize the results as follows : 1. All primers generated pands and polymorphic DNA fragments in all tested samples with the exception of WYLL44 and WYLL08 molecular markers which filled to produce any amplifiable DNA for all populations.2. The total number of alleles for all molecular markers were 119 alleles with an average of 11.9 alleles.Summaryb3. VOLP67 marker had higher genetic polymorphism, as produced 23 alleles size ranged from 161 - 244 bp.4. Rest markers showed genetic polymorphism ranged from 6 to 15 alleles.5. Observed number of alleles No ranged from 16 alleles in Najaf populations for VOLP67 molecular marker to 4 alleles in Muthanna populations for CVRL06 molecular marker.6. The total silent alleles were 51, the rare alleles were 108, frequent alleles were 177 and common alleles were 244 .7. The observed heterozygosity Ho was 1.0 for all molecular markers. The expected heterozygosity He was 0.936 in Maysan populations for VOLP67 molecular marker while it was 0.682 in Dhi Qar populations for CVRL06 molecular marker.8. Effective number of alleles Ne ranged from 2,941 in Dhi Qar populations for CVRL06 molecular marker to 10.958 in Najaf populations for VOLP67 molecular marker.9. The values of polymorphism information content PIC ranged from 0.595 in Dhi Qar populations for CVRL06 molecular marker to 0.919 in Qadisiyah populations for CMS13 molecular marker.10. The lower genetic distance and higher genetic similarity between Basrah and Najaf populations, they were 0.161 and 0.851, respectively. Higher genetic distance and lower genetic similarity between Maysan and Dhi Qar populations were 0.370 and 0.690, respectively.
