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عزل وتشخيص الفطريات المصاحبة للحامول Cuscuta spp. في البصر وامكانية مكافحته كيميائيا وحيويا == Isolation and Identification of fungi associated with dodder Cuscuta spp in Basrah and evaluation its biological and chemical control
Author name:
بيداء غازي عوفي التميمي
Supervisor name:
يحيى عاشور صالح | طه ياسين مهودر العيدان
General topic:
Agricultural sciences
Specific topic:
Plant - Prevention
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture - Department Of Plant Protection
Language:
Arabic
University location:
Basrah
First pages:
31T1769 - p.pdf
Abstract:
The present study has been conducted at the labs. Of plant protection department , and the fields of Agriculture College/ Basra university to determine the potentiality of dodder (Cuscuta planiflora Ten.) integrated control on Alfalfa plant (Medicago sativa L.) at Basra governorate.Results proued a wide range for dodder hosts , which were 38 different plant families , including three new records in Iraq which were conocarpus ; radish and leek.Twenty six different fungal species belong to twelve different genera have been180 lated from dodder stem; flower ,as well as seeds, with special emphasize on the following species : Exserohilum rostratum Bipolaris bicolar, Altrenraia longipes, A.tenuissima , Acremonium restrictum ,Bipolaris australliensis, Cladosporium oxysporiumA new record on dodder in Iraq . Hence , two species were isolated for first time in the world which were E.rostratum and B.bicolarFour different fungal species (A.terreus , E.rostratum , T. viride , A.alternata and B.bicolar ) Showed strong in hibition for dodder seed germination which were 10; 13.4 ; 16.7 and 23.4%, respectively .Fungal cultural filtrates results proved that the highest seed germination inhibition were observed at the treatments of Tricoderma viride ,Which were 38.7, 38.4 and 43.8%, respectively, while the lowest inhibition percentage was seen at E.rostratum culture filtrate treatment (13.4%) .Regarding the effect of pesticides treatments , the lowest seed germination of dodder was observed at Glyphosate treatment (36%), while a percent of 9.4% was seen at kerb pesticide .The analysis of GC - MS results revealed the existence of sereval compounds while believed to be a potential inhibitors on dodder seed germination ,such as 1,4 - Dioxin,2,3 - dihydro - 5,6 - dimethyl ,Diacetic acid , Benzoic acid ,As well as ,Ascorbic acid and a group of fatty acids.Pot results showed that the highest hight of Alfalfa plant was observed at dodder with glyphosate 350ppm treatment which was 27.56cm, hence ,the lowest average (15.34cm) was seen at dodder treatment .the highest leavesSummarynumber were observed at dodder with T.viride treatment which were 25 leaf / plant ,respectively, while , the lowest leaves number (16.78 leaf / plant )was reported at alfalfa with dodder treatment . Regarding , Alfalfa tilling parameter .the hight value was reported at dodder with T.viride with glyphosate treatment (9 tillers ) , hence ,the lowest one ( 5 tillers ) was observed at alfalfa and dodder treatment (5tillers). statistical analysis revealed that low the highest dodder severity index was observed at Alfalfa with dodder + T.viride+350 ppm of pesticide treatment (73.34%) while the lowest seventy percentage (0%) was seen at Alfalfa with dodder treatment .Field experiments showed that the highest Alfalfa hight was observed in dodder with glyphosate 350 ppm treatment (30cm), the lowest height was reported at Alfalfa with dodder treatment (21.34cm).Regaling the leaf number per plant ,Our result that the treatment of Alfalfa+dodder with T.viride showed the highest average (28.67 leaf/plant ) the lowest leaf number was observed at Alfalfa with dodder treatment (13.34 leaf/plant ).Tillering analysis proved that the treatment of dodder with T.viride scored the highest average (9tillers/plant ) , while the lowest average (5.34tillers/plant) was observed at the treatment of Alfalfa with dodder. The highest severity percentage on dodder was reported at dodder with E.rostratum +glyphosate 350ppm (100%) while the lowest percent (0%) was seen at dodder treatment .
