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تقييم الواقع البيئي لمعمل بابل /2 للبطاريات == Assessment Study For The Environmental Situation of Babylon\2 Batteries Plant

اسم المؤلف: منال مالك سعدون الموسوي
اسم المشرف: سهير ازهر الكاظمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In this study was investigated the quantity and quality of industrial water from (Babil/2 batteries plant in Baghdad) and estimate the amount of air pollutants for some factory sections and estimate the amount of solid waste resulting from the industry and study the impact of these pollutants on the environment and public health.The first target of this study is investigated the physical and chemical characteristics of the discharged water from sections shipping and ficus where the sample water collocated was two samples per month for 6 month was taking during period from Novembers 2013 to May 2014. Some measurements were conducted laboratory and others on site, the result showing the following values of the variables rates for the shipping and ficus sections before treatment and respectivelyResults of the study showed that there is a significant increase inThe concentrations in sulfate and TDS, heavy metal When reached after treatment for the same variables and respectivelypH 9.9, EC 1524.9 ?s/cm, TSS 166.4mg/l, TDS 611.7mg/l, Cl - 64.46mg/l, (SO4)+2 486.8mg/l, BOD 49.2mg/l, COD 220.4mg/l, Pb+2 0.55mg/l, Zn+2 0.09mg/l, Cd+2 0.01mg/lpH 8.2, EC 1191.6?s/cm, TSS 68.1mg/l, TDS 927.6mg/l, Cl - 76.95mg/l (SO4)+2, 722.6mg/l, BOD 4.4mg/l, COD 29.2mg/l, Pb+2 0.04mg/l, Zn+2 0.03mg/l, Cd+2 0.01mg/lFrom the results of the study showed that there is a no significant increase in the concentrations in sulfate and TDS, but increases slowly. In the water of charging department reached the concentration of same variables and respectivelyThe second aims of this study a steamed the concentration and rate of air pollution (Total Suspended Particles, heavy metal, Oxides and gases) collected the sample of outstanding minutes analysis made to know and identify the concentration of heavy metal for same factory section and compared those with local and global determinants also the concentration of air emitted was measured.Results of the study showed that there is a significant increase in the concentrations of the outstanding minutes compared to the allowable limits as it reached the highest concentration in the Department of Shipping 3313.46?g/m3. The lowest concentration was in the section of the Aggregation 0.8155?g/m3. Where the concentration of heavy metals, especially lead element exceeded the permissible limits as it reached the highest concentration in Ficus section 3.53?g/m3 the lowest concentration in the assembly department which is 0.36 ?g/m3 concentration of CO gas in the department of Ficus, Shipping, assembly, queries reachedpH 1.6, EC 12058?s/cm, TSS 148.3mg/l, TDS 10122mg/l, Cl - 76mg/l(SO4) +2 3838mg/l, BOD 23.55mg/l, COD 203.5mg/l, Pb+20.55mg/l Zn+20.46mg/l, Cd+2 0.022mg/lrespectively.(21.7, 3.9, 2.44, 2.84?g/m3), the concentration of CO2 was 231.8, 187.6, 219.4, 225.4?g/m3.In the department of Ficus, Shipping, assembly, respectively queries either NO2 was 0.12?g/m3 in sections of Ficus, Shipping and queries, 0.16?g/m3 in the assembly department. The H2S appeared only in sections Ficus, Shipping with concentration 0.02?g/m3, 0.18?g/m3 respectively, either gas SO2 only appeared in the shipping department and was only 0.01 ?g/m3.The last part of these study was to calculate the amount of solid waste for a period of study in terms of total solid waste generated by the plant was 37.004 tons for eight months and included this waste pastes lead and lead plates and scrap processing units either the amount of recycling of batteries that are broken in the foundry of lead in Khan Dhari amounted to 354.832 tons for eight months, and the output of 181.1 tons. In this study we foxed on the problems in these factory that effected the process of industry and we found liquid wastes in the factory is not efficient in reducing the concentrations of all the variables treatment and gaseous emissions generated in the factory, there are no have any significant treatment of solid waste recycled and are utilized as it emerged that the Babil / 2 battery plant on a large degree of pollution sulfate and TDS with regard to liquid waste. The minutes and the outstanding lead with respect to gaseous pollutants to the current study that recommended a set of recommendations that would work to eliminate or minimize the causes of pollution and thus access to good results.

الكشف عن بعض عوامل البيئة الداخلية للخلايا السرطانية للنساء العراقيات المصابات بسرطان الثدي == Detection of Some Microenvironment Factors In Tissue Samples of Iraqi Women With Breast Cancer

اسم المؤلف: فاطمة سمير عبد الرزاق الزبيدي
اسم المشرف: اخلاص مشرف عيدان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Breast cancer (BC) is the most common cause of cancer - related deaths in women. It continues to rank as one of the top killer of women. This cancer increased in frequency in the last years in Iraq. The aim of this study was to shed light on the immunohistochemical for some factors that could be affect on development of microenvironment in breast cancer of Iraqi patients. And these factors include CD133 as a marker for breast cancer stem cells (BCSs), and also studying tumor necrosis factor alpha (TNF - ?) and transforming growth factor beta (TGF - ?). 53 samples Formalin - fixed, paraffin - embedded (FFPE) wax block appeared their ages were range from 29 - 70 year with a mean age of 48.45 years. 32 patients with breast carcinoma and 21 patients with benign breast lesions were included in this study for comparison. The results of this study show that the CD133 positive expression was found in (56.2%) of Iraqi breast cancer cases. Also the result of this study show that (62.5%) positive expression from both (TNF - ? &TGF - ?) of breast cancer cases, compared with sample of benign breast lesion. The results show (52.3%) positive expression of TNF - ? and (28.57%) positive expression of TGF - ? of samples with benign breast lesion, there is a significant different between studied samples, compared with (19.05%) samples positive expression of CD133 of sample with benign breast lesion so there is a significant different between the samples with breast cancer and benign breast lesion. Also the results show there is a positive relationship correlation between (TGF - ?) expression and (TNF - ?) expression, while there were no relationship correlation between (TGF - ?) and CD133 and no relationship correlation between TNF - ? and CD133. The results show there is a positive correlation between the grade and breast cancer with the three different expressions of marker but in different strongest correlations between (TNF - ?) and (TGF - ?) with the graded but this correlation becomes weak with CD133 marker the value of significant. According to the relationship in breast cancer case in this study between the studied markers and stage of case it shows (TNF - ?) has a strong positive correlation while the correlation appear week between the stage of this studies case and end each of TGF - ? and CD133. For this we concluded from the results there high expression of CD133 and TNF - ? indicators and TGF - ?. CD133 could use in diagnosis of the cancer cell and the high expression of TNF - ? & TGF - ? indicate that these factor play important roles in tumor microenvironment metastasis. And the strong correlation between the expression of these markers with grade and stage of breast cancer

دراسة بعض الجوانب المناعية والبكتريولوجية لمرضى ذات الرئة Pneumonia == Study Some Immunological And Bacteriological Aspects of Pneumonia Patients

اسم المؤلف: كرم رياض حسن الجراح
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت 120 عينة قشع من اشخاص مصابين بمرض ذات الرئه للمدة من ايار 2013 الى شباط 2014 من ثلاثة مستشفيات في مدينة بغداد وشملت مستشفى اليرموك التعليمي, مستشفى دار التمريض الخاص, مستشفى بغداد التعليمي. شخصت العينات باستخدام الفحوص البايوكيميائية, نظامApi 20 E و| From May 2013 to Feburey 2014, 120 sputum and Aspiration samples of patients with pneumonia disease were collected from different hospitals in Baghdad included : Al Yarmouk Teaching Hospital, Hospital Nursing Home sector and Baghdad Teaching Hospital. All samples were diagnosed by biochemical tests, Api 20 E and Api20 strep. Systems, The results was found to be 28 isolates (23.3%) belong to K.pneumoniae, 26 isolates (21.7 %) belong to S.pneumonia while 66 isolates(55%) belong to causes : E.coli, Pseudomonas sp., Moraxella catrrhalis, S.pyogens, Monilia and S.aureus. From (55) blood samples of pneumonia patients and 30 blood samples from healthy individuals, number of white blood cell (Neutrophil) counts were measured, percentage of Neutrophil cell count in female (53%) higher than the cell count in male(47%). Some markers of pneumonia disease were studied such as ratio of infection between male and female, chronic and acute cases and smoker and non smoker, the results were showed the ratio of infection in female more than in male, (60%, 40%), respectively. And number of acute cases of pneumonia patients 41(74.55%) more than chronic cases 14(25.45%). While infection ratio in smoker patients more than nonsmoker patients at ratio 24(57.14%)18(42.86%), respectively. The result of interleukin - 8 level through acute and chronic phases in pneumonia patients sera was showed high level in patients compared with healthy persons (86.4403 ± 25.50919 vs. 58.7636 ± 4.73152 pg / ml, respectively) with a non significant difference statistically (P?0.05). Also in the age group (age 15 - 60years), The results showed the results interleukin 8 levels higher than the age groups of (2month - 3years) and (age 60 - 85) (93.63 ± 51.65, 68.88 ± 17.17, 65.68 ± 11.73 pg / ml, respectively) with non significant difference (P?0.05). The result of Leukotriene B4 level through acute and chronic phases in pneumonia patients sera showed high level in patients compared with healthy persons (36.00 ± 3, 82 vs. 25.96 ± 4.44 pg / ml, respectively) with a significant difference (P?0.05). Also in the age group (two months - 3 years) were showed the results interleukin 8 levels higher than the age groups (age 15 - 60) (age (60 - 85)(33.61 ± 11.06, 19.29 ± 4.67, 16.86 ± 4.51 pg / ml respectively) with no siginificant difference (P?0.05), the correlation coeifficient between interleukin - 8 and leukotriene B4 was (0.12) with no siginificant difference. The phagocytic activity was determined in pneumonia patients sera according to measurement C3 protein level through acute and chronic phases, the result were showed the ratio of C3 protein levels in healthy persons more than in patients (135.93 ± 12.74, 133.19 ± 12.01 pg / ml, respectively) with a difference was not significant statistically (P?0.05).

التنميط الوراثي لمستضدات التطابق النسيجي في عينة عراقية من مرضى السكري النوع الثاني == Hla Genotyping In A Sample of Iraqi Type 2 Diabetes Mellitus Patients

اسم المؤلف: احمد كاظم محمد
اسم المشرف: محمد ابراهيم نادر | بتول حسن الغرابي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: داء السكري مرض واسع الانتشارعالميا تختلف نسبة الاصابة بين البلدان المتطورة والنامية ويعتبر المسبب الرئيسي للاعاقة والموت في العالم.داء السكري النوع الثاني هو الاكثر حدوثا حيث تبلغ نسبة الاصابة (90 - 95%) من مجموع المصابين بالسكري للانواع الثلاثة الرئيسية. | Sixty of non insulin dependent diabetes mellitus (NIDDM) patients who were diagnosed according to American Diabetes Association criteria (ADA) 2007 were selected from the specialized center of endocrinology and diabetes (Baghdad AL - Russafa Health Directorate) during the period between first of May 2013 to last of October 2013.Their age ranged 35 - 70 years. Fourty apparently healthy individuals their age ranged (35 - 70) years were considered as control. Enzymatic colorimetric methods used for measured FBS (fasting blood sugar) and HbA1c (glycohemoglobin) and enzyme linked immunosorbent assay (ELISA) for hormones and enzyme markers. Fasting blood sugar revealed high significant in patients with median (11.6 mmol/L vs. 5.9 mmol/L) and (P<0.001) in comparison to control groups.Elevation of glycated hemoglobin (HbA1c) with mean (9% vs. 5.5%) in comparison to control groups. Another reliable marker are the concentration Adpionectine hormone, Insulin hormone and adenosine deaminase activity the results of those estimated significantly difference between levels mean (20.7 vs. 34 ng /ml) in patients compared to healthy subjects (P<0.001) for adiponectin ; mean (106.6 vs. 59.3 ng/ml) for ADA (adenosine deaminase) with (P<0.001) and the median (12.1 vs. 16 uIU/ml) for insulin hormone with (P 0.001). In order to investigate the accuracy of previously mentioned tests, a statistical analysis [Receiver - Operator Characteristics (ROC)] has been applied to show the accuracy, specificity and sensitivity of the tests under test.This analysis revealed that serum ADA activity is the best marker with highly specificity 100%, sensitivity 100%, and accuracy 100% while; FBS was the best test with highly specificity 100%, sensitivity 100% and 100% accuracy in comparison with other tests. It was denoted that type2 DM was associated with certain HLA class II alleles were analyzed for their genotyping by Polymerase Chain Reaction - Sequences Specific Olegionucleotide (PCR - SSO) technique. The present study revealed that diabetic patients were positively associated with HLA - DQB1*0201 (83% vs. 5.0%) which is the most prevalent in patients followed by DRB1*1137 (46.7% vs. 0.0%); DRB1*0401 (41.7% vs. 2.5%), and DRB1*1306(15% vs.0.0%) while HLA, A*0201;B*3559;Cw*0410 and DQB1*0501 is negatively associated in type 2DM in comparison with healthy control groups.This study has shown that there is no significant association between FBS, HbA1c, serum insulin, HOMA2(Homeostatic Model Assessment2) ? - function, HOMA2 - IR, serum adiponectin, serum ADA and HLA alleles(DQB1*0201, DRB1*1137, DRB1*0401, DQB1*0501, DRB1*1306) in spite the significant associated between FBS and serum ADA and HLA - DRB1*0701 allele with (P 0.021, P 0.008) respectively.The current result concluded that there may be an important role for HLA genotyping in arising the chance for enhancing the susceptibility for either disease development or protection against its initiation.

انشاء وتوصيف لخط سرطان الثدي الخلوي العراقي == Establishment And Characterization of Iraqi Breast Cancer Cell Line

اسم المؤلف: مرتضى عادل الشامي
اسم المشرف: محفوظة عباس عمران | احمد مجيد الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Several primary cultures were initiated from different samples obtained from Iraqi female patients of breast tumor, one sample was successful, and this sample was histological diagnosed as breast cancer infiltrating ductal carcinoma.The cell suspension was cultured in tissue culture flask and confluent monolayer achieved after 16 days from primary culture. The continuous subculture was done in grown cells in tissue culture flask each 48 - 96 hrs. Between subculture to other until across 50 passages through11months.In our current study different experiments were done to characterize the cultured continuous cells, which are studying the growth curve of the new established cell line and calculating the population doubling time that have been 22 hours.Furthermore, a morphological study was carried out by staining the cells with hematoxilin and eosin dyes. The cells were elongated multi - polar epithelial like cells with nuclear polymorphism and multi - nuclei, in addition to high nuclear to cytoplasm ratio, all these characters of the malignant tumor cells.The Cytogenetic study showed chromosomal aberrations with many numerical changes among the tumor cells and abnormal structure gives chromosomes with unknown origin called marker chromosome. In furthermore the G - band stained of normal 46XX chromosome was done to facilities the comparisons between chromosomes of the new established breast cancer cell line and normal chromosomes aberrationsImmunocytochemistry examination was done for the tumor cells grown in multi well tissue culture slide chamber to detect the presence of some hormonal receptors (ER and PR) gives negative result, and to test Her - neu2 gene that gives week positive result.

تاثير فطريات المايكو راي زا الشجيرية والمادة العضوية في مرض الذبول المتسبب عن الفطر Fusarium oxysporum f.sp.lycopersici في نبات الطماطة Lycopersicon esculentum == Effect of Arbuscular Mycorrhiza And Organic Matter On Wilting Caused By (Fusarium Oxysporum F.Sp. Lycopersici) In Tomato (Lycopersicon Esculentum)

اسم المؤلف: ثامر عبد الشهيد محسن علي الابراهيمي
اسم المشرف: بتول زينل علي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The study was conducted to evaluate the effect of three arbuscular mycorrhizal species (Glomus etunicatum, G. leptotichum, Rhizophagus intraradices) and the organic matter (Peatmoss) on controlling the wilting caused by Fusarium oxysporum f.sp.lycopersici on tomatoes using pot cultures. The study was included two experiments : 1 - The first experiments was performed to select the best mycorrhizal species or a maxture of two or three species acting on several parameters, Results showed the followings : - Significant increase of the tri mixture activity of the three mycorrhizal species compared to the dual mixture or single species on mycorrhizal colonization, mycorrhizal dependency and the weight of mycorrhizal roots after four and eight weeks planting. - The highest activity of the trimixture of AM fungi in increasing the plant growth parameters after four and eight weeks planting. - The highest activity of the tri mixture of AM fungi in increasing the percentage of N, P, K and the protein in leaves and roots after 8 weeks planting compared to other treatments.2 - The second experiment was performed to study the effect of the tri mixture of AM fungi (which gave the highest activity in the first experiments) and the organic matter in the presence of the pathogen F.o.l. and their interactions on mycorrhizal parameters, disease parameters, analysis of some elements and compounds, study of some plant growth parameters, enzymatic and non - enzymatic antioxidants in tomato plant, Results revealed the followings : - Significant increase in mycorrhizal colonization included % of mycorrhizal frequency, mycorrhizal intensity in the root system, mycorrhizal intensity in in root fragments, abundance of arbuscules in parts of root fragments, abundance of arbuscules in root system and the highest values were shown in the treatment (organic matter x 4weeks pathogen infection) after six and ten weeks planting. - Significant increase in dry weight of mycorrhizal roots, the highest was shown in the treatment (organic matter x control) after 10 weeks planting. - Increase in percentage of mycorrhizal dependency after 6 and 10 weeks planting and the highest percentage was shown in the treatment (absence organic matter x control) after 10 weeks. - Increase in number of mycorrhizal spores, the highest was recorded in the treatment (organic matter x 4weeks pathogen infection) after 12 weeks. - Significant decrease in % of disease parameters in leaves and roots, the lowest percentage were recorded by the treatment (trimixture of mycorrhiza x organic matter x 4weeks pathogen infection) for all parameters. - Results of plant growth parameters showed significant increase of all for different weeks. The treatment (trimixture of mycorrhiza x organic matter x control) recorded the highest for all parameters tested in plant. - The treatments affected the flowering and fruit duration, the shortest was shown by the treatment (trimixture of mycorrhiza x organic matter x control) - Significant increase in percentage of N, K and protein in leaves and roots, and chlorophyll a, b and total chlorophyll in leaves, the highest values were shown in treatment (trimixture of mycorrhiza x organic matter x control). - The treatment (trimixture of mycorrhiza x organic matter x 4weeks pathogen infection) recorded the highest percentage of P, souble carbohydrates, highest content of malondialdehyde and hydrogen peroxide in leaves and roots as well as highest percentage of lignin in roots. - Significant increase in the activity of antioxidant enzymes in leaves and roots, the highest activity recorded by the treatment (trimixture of mycorrhiza x absence organic matter x 4weeks pathogen infection). - Significant increase of non - antioxidant enzymatic in antioxidants included content of carotene in leaves, content of ascorbic acid, proline, total phenol, and glutathione in leaves and roots, The treatment (trimixture of mycorrhiza x organic matter x control) showed the highest content of carotene and ascorbic acid while the treatment (trimixture of mycorrhiza x organic matter x 4weeks pathogen infection) showed the highest content of proline, total phenol and glutathione in leaves and roots.

دراسة تصنيفية لانواع مختارة من نباتات ذوات الفلقتين في محافظة كربلاء المقدسة, العراق == Taxanomical Study For Selected Dicot. Spp. In The Holy Karbala Province, Iraq

اسم المؤلف: زينة خليل ابراهيم البهادلي
اسم المشرف: علي حسين عيسى الموسوي | خليل ابراهيم الشمري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة بيئية لعدة مواقع من محافظة كربلاء المقدسة بين شهري شباط وايار ولسنتي 2013 و2014 من حيث نوع الترب والمجتمعات الاحيائية لهذه المواقع جمعت خلالها انواع مختلفة من النباتات تابعة لعائلات مختلفة ضمن ذوات الفلقة الواحدة وذوات الفلقتين اذ | The present work includes ecological study for different locations of the holy Karbala province between February and May and for the years2013, 2014, investigating soil kinds and communities in these locations. Different plant species of Monocots and Dicots were collected from variable soil kinds, such as sandy saline soil, loamy saline soil and sandy gravely soil. The species of Haloxylon salicornicum, Zygophyllum coccenium were dominant in the communities of sandy saline soil, Nitraria retusa, Juncus sp. and Halocnemum strobilaceum were dominant in loamy saline soil.Comparative anatomical study has been done for (30) Dicots wild species grown in these environments of Karbala province, as follow : a.Family : Aizooaceae1) Aizoon hispanicum L. b.Family : Chenopodiaceae2) Chenopodium murale L.3) Halocnemum strobilaceum (Pall.)M.Bieb.4) Haloxylon salicornicum (Moq.)Bunge ex. Boiss.5) Salicornia herbacea L.c. Family : Compositae (Asteraceae)6) Carthamus oxycantha M.B. 7) Launaea capitata (Spreng.)Dany 8) Launaea mucronata (Forssk.)Muschl. 9) Rechardia tingitana (L.)Roth. d. Family : Convolvulaceae10) Convolvulus oxypyllus Boiss. e. Family : Cruciferae (Brassicaceae)11) Diplotaxis harra (Forssk.)Bioss. 12) Sterigosella africana (L.)Boch. 13) Sterigosella grandiflora (Bnge)Bochantsev 14) Savignya parviflora (Del.)Webb. 15) Neotorularia torulosa (Desf.) Hedge & J. Leonard 16) Zilla spinosa (Turra)Prantl. f. Family : Cucurbitaceae 17) Citrullus colocynthis (L.)Schrad. g. Family : Geraniaceae18) Erodium laciniatum (Cav.) Willd.h. Family : Malvaceae19) Malva parviflora L. i. Family : Neuradaceae 20) Neurada procumbens L. j. Family : Nitrariaceae21) Nitraria retusa (Forssk.)Aschers.22) Peganum harmala L. k. Family : Papillionaceae23) Trigonella stellata Forssk. m. Family : Polygoniaceae 24) Rumex versicarius L. n. Family : Resedaceae25) Oligomeris linifolia (Vahl)Macbr.q. Family : Solaneaceae 26) Lycium barbarum L.r. Family : Tamaricaceae 27) Tamarix florida Bge.28) Tamarix macrocarpa (Ehrenb.)Bge.u. Family : Zygophyllaceae29) Fagonia glutinosa Del. 30) Zygophyllum coccenium L. The present research focused on many anatomical characters and studies comparatively, such as epidermis types, stomatal complexes, leaf venation, cross sections of root, stem and vertical sections of leaf blades. Different cross sections were shows variability cuticle thickness and epidermis, vascular cylinder tissues, collenchyma and sclerenchyma in different species studies. Variations were noticed among these tissues in the variable species.Thickness of cuticle epidermis of leaves were variable, and unifacial and bifacial mesophylls were noticed for the first time in some species studies. Some cross sections were obtained for scale leaves in Tamarix florida, Tamarix macrocarpa, Halocnemum strobilaceum, Haloxylon salicornicum and Salicornia herbacea.The anatomical characters of these plant parts has not been studied before for these species, at least for Iraqi specimens. The following species Launaea capitata, Oligomeris linifolia and Zilla spinosa were glabrous in all vegetative and reproductive organs. In time that the other species, Haloxylon salicornicum , Salicornia herbacea, Launaea mucronata, Reichardia tingitana, Strigosella grandiflora , Peganum harmala, Lycium barbarum, Tamarix florida and Tamarix macrocarpa were glabrous just in the vegetative parts, but hairy (pubescent) in the reproductive organs. The indumentums was variable in thickness within variable plant organs and parts, even on the lower and upper leaf surfaces. Different kinds of crystals were found and verified in the stems and leaves of different species. Size and density of these crystals were variable in variable locations of stem and leaf tissues.. Geographical distribution was investigated for all the studied species using Iraqi satellite maps by GIS program. Neotorularia torulosa was the most distributed species, and Rumex versicarius was the least distributed one. Schedules, schemes, illustrations and micrographs were presented for the different species studied in this work. Lastly results were discussed depending on available references and reasons.

دراسة تصنيفية لانواع الجنس Echinops L. (Compositae) في العراق == Comparative And Systematical Study of The Genus Echinops L. (Compositae) In Iraq

اسم المؤلف: سكينة عباس عليوي
اسم المشرف: علي حسين عيسى الموسوي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تصنيفية مقارنة لانواع الجنس Echinops L. في العراق حيث شملت الصفات المظهرية والتشريحية وحبوب اللقاح، وكذلك البيئة التوزيع الجغرافي فضلا عن المعاملة التصنيفية. شملت الدراسة المظهرية دراسة صفات الجذور والسيقان والاوراق والرؤوس الزهري | The present investigation dealt with comparative systematic study of the genus Echinops L. (Compositae) in Iraq.The work involved comparative morphological and anatomical characters, in addition to palynology, ecology, geographical distribution and taxonomic treatment for these species. Morphological characters of roots, leaves, involucre bracts, inflorescence, in addition to, indumentum variations were discussed and the study concluded that stems, leaves, involucres bracts, especially the inner most layer characters were useful aid in distributing species of the genus. Comparative anatomical characters were used, such as characters of leaves, epidermis stomatal complexes, variation patterns, cross sectionings of stems, leaves as well as L.S of growing points. The study revealed that the anatomical characters are important taxonomically. The present investigation observed that pollen grains of all species were tricolporate and echinate. The taxonomic important of grains characters were assessed of the species. Maps of geographical distribution were prepared for all species studied. The study shown that most species were distributed in the North area of Iraq and a few species of the genus were distributed in the East and South of Iraq. In conclusions, (11) species of the genus Echinops were recognized in Iraq. Taxonomic treatment was carried out for all Echinops species and key was designed for them

دراسة مقارنة للتاثير السمي للزنبور الاحمر Vespa Orientalis L والزنبور الاصفر Polistes Olivaceaus (De Geer) (Hymenoptera : Vespidae على تحلل كريات الدم الحمراء والتخثر لدى الانسان == Poison Effects of Red Wasp Vespa Orientalis L. And Yellow Wasp Polistes Olivaceaus (De Geer) (Hymenoptera : Vespidae) On The Decomposition of Red Blood Cells And Coagulation In Humans

اسم المؤلف: شهد فلاح عباس
اسم المشرف: سعدون ابراھیم اسماعیل | محمد عبد الرزاق الصوفي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: To study the impact of toxic of the the Red Wasp Vespa orientalis L. and yellow wasp Polistes olivaceaus (De Geer) from family (Vespidae) the decomposition of red blood cells and clotting in humans. Were collected 508 insect Red wasp was where the number of workes 390 insect the yellow wasp gathered 539 insect where the number of worker 418 insect, and the diagnosis was confirmed based on the taxonomic keys and professors speclalized in the natural history museum and then extracting machine stinging for poisonWasp red and yellow toxin were purified by gel filtration throw Sephacryl S - 200 were obtained on three peaks of proteins, and when estimated the activity of lysis and clotting for peaks separate P1, P2 and P3 for wasp red toxin, the lysis activity of separate P1 was 98% of the poison origin activity and the clotting time was 47.3 minutes, while the lysis activity of separate P2 was 62% of the poison origin activity and the clotting time was 27.53 minutes, and the lysis activity of separate P3 was 29% of the poison origin activity and the clotting time was 13.67 minutes, so P1 separate from Wasp red poison was selected to purification and study the molecular characteristics, while when estimated the activity of lysis and clotting for peaks separate P1, P2 and P3 for wasp yellow toxin, the lysisactivity of separate P2 was 91% of the poison origin activity and the clotting time was 43.8 minutes, while the lysis activity of separate P1 was 49% of the poison origin activity and the clotting time was 19.28 minutes, and the lysis activity of separate P3 was 29% of the poison origin activity and the clotting time was 7.52 minutes, so P2 separate from wasp red poison was selected to purification and study the molecular characteristics The molecular weight of wasp red and yellow toxin the protein cm wasp red toxin P1 was 22387 Da and wasp yellow toxin P2 was 22382 Da using the gel filtration Sephacryl S - 200, while the carbohydrates content were 60.057 and 44.460 mg/ ml for wasp red P1 and yellow P2 toxin respectively as determined by phenol - sulfuric acid method, and type of link between carbohydrates and protein in the separate P1 and P2 investigating by using gel filtration Sephacryl S - 200 and absorbance at 280 and 490 nm.The percentage of iron in wasp red P1 and yellow P2 toxin were 0.88 and 0.44 ppm respectively as determined by atomic absorption, the contain proteins separate P1 and P2 on the iron were investigating by using gel filtration Sephacryl S - 200 and absorbance at 280 and 557 nm. The optimum pH of lysis and clotting activity for wasp red toxin P1 was 6.5, while lysis activity was decrease in pH 4 and 8 to be 38 and 79% respectively from its original activity, and clotting time was decreased from 47.3 min at optimum pH to be 17.9 and 37.3 min at pH 4 and 8, respectively, it was noted that wasp red toxin P1 was constant in the pH range of lysis activity from 6 - 7.5, but it lost 67 and 25% from its original activity at pH 4 and 8 respectively, while the clotting time was constant in the pH range from 6 - 7.5, but it decreased in pH 4 and 8 to be 15.6 and 35.4 minutes respectively The optimum pH of lysis and clotting activity for wasp yellow toxin P2 was 6.5, while lysis activity was decrease in pH 4 and 8 to be 18 and 37% respectively from its original activity, and clotting time was decreased from 48.8 min at optimum pH to be 8.7 and 35.6 min at pH 4 and 8 respectively, it was noted that wasp red toxin P2 was constant in the pH range of lysis activity from 5.5 - 8, but it lost 74 and 19% from its original activity at pH 4 and 8 respectively, while the clotting time was constant in the pH range from 6 - 7.5, but it decreased in pH 4 and 8 to be 12.6 and 39.5 minutes respectively The results for optimum stability temperature of lysis and clotting activity for wasp red toxin P1 refer to increase activity by increasing the action temperature until it reached a maximum at a temperature 35oC, then lysis activity was decreased to be about 4% of its original activity at 80oC, while the clotting time was decrease from 48.8 minutes at the optimum temperature to be 1.8 minutes at 80oC, it was noted that the lysis and clotting activity were stabile at 35oC for 30 min, but it is beginning to decrease with increasing temperature to loses 84% of its original lysis activity at 80oC, and the clotting time was decreased from 47.3 min at 35oCto be 2.3 min at 80oC.The results for optimum stability temperature of lysis and clotting activity for wasp yellow toxin P2 refer to increase activity by increasing the reaction temperature until it reached a maximum at a temperature 35oC, then Lysis activity was decreased to be about 8% of its original activity at 80oC, while the clotting time was decrease from 48.8 minutes at the optimum temperature to be 3.9 minutes at 80oC, it was noted that the lysis and clotting activity were stabile at 35oC for 30 min, but it is beginning todecrease with increasing temperature to loses 98% of its original Lysis activity at 80oC, and the clotting time was decreased from 48.8 min at 35oC to be 5.3 min at 80oC.The results refer to found a relationship between the influence of the wasp type and dose on the red blood cells lysis degree in males and females, it was observed that the concentration had a significant effect (p<0.05) in the red blood cells lysis degree in females, it was 0.401 at concentration 100% for wasp red toxin P1, while it was 0.331 at the same concentrate for wasp yellow toxin P2, the concentration also had a significant effect (p<0.05) in the red blood cells lysis degree in males, it was 1.020 at concentration 100% for wasp red toxin P1, while it 0.842 at the same concentrate for wasp yellow toxin P2, also it refer to found a significant effect (p<0.05) between the effect of the wasp red P1 and yellow P2 toxin and dose on the red blood cells clotting time in females, itwas 46.80 min at concentration 100% for wasp red toxin P1, while it was 46.20 min at the same concentrate for wasp yellow toxin P2, the concentration also had a significant effect (p<0.05) in the red blood cells clotting time in males, it was 47.80 min at concentration 100% for wasp red toxin P1, while it 40.47 min at the same concentrate for wasp yellow toxin P2, there was no significant effects for blood clotting time when making a comparison between the wasp red P1 and yellow P2 toxin for eachconcentration in females and males. The compared between red blood cells clotting time due the wasp red P1 and yellow P2 toxin dose in females and males did not refer to found a significant effect (p<0.05), the clotting time for wasp red P1 was 46.80 and 47.80 min at concentration 100% for females and males respectively, while the clotting time at concentration 6.25% was 17.30 and 17.10 min for females and males respectively, whilst the clotting time red blood cells due wasp yellow P2 toxin in females was 46.20 min and 47.40 min in males at concentration 100%, and it was 17.00 min in females and 17.10 min in males at concentration 6.25%.

دراسة تصنيفية مظهرية لبعض انواع عائلة فراشات اللهانة من رتبة حرشفية الاجنحة (Lepidoptera : Pieridae) من مناطق وسط العراق وشماله == Taxonomic Morphological Study of Some Species of Cabbage Butterflies, (Lepidoptera : Pieridae) From Mid And North Regions of Iraq

اسم المؤلف: مهند محمود اغا
اسم المشرف: حسن سعيد جاسم الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study comprises taxonomic morphological of seven species belong to six genera of the subfamilies Pierinae, Coliadinae, (Pieridae : Lepidoptera) which were collected from middle and north of Iraq.The described species in this study are : Famliy : Pieridae Duponchel - Subfamily : Pierinae DuponchelGenus : Pieris Schrank, 1801Pieris rapae Linnaeus, 1758Pieris brassicae Linnaeus, 1758Genus : Belenois Moor, 1881Belenois aurota Fabricius, 1793Genus : Colotis Hübner, 1816Colotis fausta Olivier, 1804Genus : Anthocharis Boisduval, 1836Anthocharis cardamines Linnaeus, 1758 - Subfamily : Coliadinae SwainsonGenus : Colias Fabricius, 1807Colias croceus Fourcroy, 1785Genus : Gonepteryx Leach, 1785Gonepteryx rhamni Linnaeus, 1758The last species as new recorded to fauna of Iraq

التمنيع باضداد الخمل النوع الاول المنتجة من بكتريا الاشريكية القولونية المسببة لالتهاب المجاري البولية ضد استيطان واصابة المثانة == Immunization With Type 1 Fimbriae of Uropathogenic Escherichia Coli Against Colonization And Infection of Bladder

اسم المؤلف: اروى علي شكر
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Between September and November 2013 a total of 140 urine specimens obtained from patients in Al - Kadhmiah Teaching Hospital, Ibn - Al Naffees Teaching Hospital, and Educational Laboratories in Medical city. These midstream urine specimens were obtained from patients clinically suspected to have urinary tract infection (UTI) in sterilized containers. All specimens were processed immediately after collection.A total number of 140 specimens of mid - stream urine were collected from patients suffering urinary tract infection symptoms. These isolates were obtained from 41 male and 99 female patients. They were distributed in the age group of 5 - 60 years old. All specimens were identified using biochemical tests and API 20 E system. 60 (42.85%) of urine specimens was Escherichia coli and 15(10.71%) of specimens growth was Klebsiella spp. and 25(17.85%) growth was Proteus spp. and 40 (28.57%) was G+ve bacteria.The adherence ability of E.coli isolates were evaluated by using Congo red agar and detected their ability to produce biofilm by using methylene blue staining technique in polystyrene microtiter plates and then Optical density was determined at 580 nm.All sixty isolates of E.coli were grown on Congo red agar medium to detect their adherence ability. Twenty (33.3%) isolates were given negative result by forming pink colonies on congo red agar, were as, forty (66.6%) isolates were given positive result by forming black colonies with a dry crystalline. Three isolates give strong results E.coli (13, 8, and 40). Consistency indicated biofilm production by microtiter plate. E. coli 40 which isolated produced the thickest biofilm (O.D. : =3.17).Type 1 fimbriae expression by E.coli 40 isolate was detected by mannose - resistant hemagglutination (MRHA) of human blood group (O) IRBCs in the presence of 2% D - mannose. The isolate showed strong MRHA characteristics of type 1 fimbriae under microscope (40x). The E.coli E.40isolate which produced the thickest biofilm and gave strong MRHA was elected to be the source of type 1 fimbriae.Type 1 fimbriae was extracted by heating and mechanical shearing and partially purified by Ultracentrifugation (48, 000xg for 3hrs). SDS - PAGE with a molecular weight 20, 000 Dalton.Anti - type1 fimbriae antisera were prepared in rabbits. The specificity and titration of anti - type1fimbriae antibodies were determined using IgG protein by radial immunodiffusion plate and bacterial agglutination. IgG antibodies to fimbriae type 1 were also detected in rabbit sera from immunized rabbit and non - immunized. Immunized rabbit test 1 had increases in Fim1 - specific IgG antibodies (9.5) mg/dl. Immunized rabbit test 2 had increases in Fim1 - specific IgG antibodies (8.6) mg/dl. The non - immunized rabbit had no increase in absolute IgG antibodies to Fim1.The antisera agglutinated bacterial cells up to 1/80 dilution confirming the presence and titer of specific antibodies against type1 fimbriae.The microtiter plate method was applied to estimate the role of the prepared anti - type1fimbriae antibodies in reducing the biofilim formation by E.coli. Prevention of bacterial adherence and subsequent biofilm formation to polystyrene microtiter plate was studied by employing different dilutions (1/10, 1/20, 1/40, 1/80, 1/160, 1/320, 1/640) of rabbit sera containing anti - type 1 fimbriae antibodies. The maximum inhibition of biofilm formation in terms of optical density (580 nm) was found in lowest dilution (1/10) (highest concentration of Abs). However, the minimum inhibition was observed in highest dilution of rabbit antisera (1/640) (lowest concentration of Abs). Here the inhibition occurs in a dose dependent manner as the biofilm formation increased dramatically with increasing in antisera dilution. The results also showed that there is a significant difference(P<0.05) among data treated with sera and data without sera and among serial dilutions.General urine examination and culture for urine that absorbed from test 2 and control was done (GUE) show that pus cell in urine was (1 - 2/HPF). There was no growth of bacteria in urine culture.The Histological section show that the Control and test 1 rabbit (have been immunized with fimbriae and adjuvant and injected with E.coli intra muscular) and Test 2rabbit (that has been immunized with fimbriae and adjuvant and injected with E.coli directly in bladder has normal epithelial cells and mucosa.

التقييم الجزيئي لنسخ جين المقاومة الدوائية MDR1 في بعض المرضى العراقيين البالغين المصابين بسرطان ابيضاض الدم الحاد == Molecullar Assessment of Multidrug Resistance Gene (MDR1) Transcript In Some Adult Iraqi Patients With Acute Leukemia

اسم المؤلف: كفاح جبار شاكر اليعقوبي
اسم المشرف: عبد الحسين الفيصل
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study aims to shed light on the follow up of acute leukemic (AL) patients at initial diagnosis and after treatment to assess the response and early relapse through evaluating the gene expression level of one of the major multidrug resistance genes which is the multidrug resistance 1 (MDR1) to investigate the possible association between level of MDR1 gene expression and the clinical outcomes and this may be considered as a potential marker for response to chemotherapy of acute leukemic patients. Furthermore, the current study correlates between the MDR1 gene phenotype and MDR1 genotype in three important coding regions (C1236T, G2677T/A, and C3435T considering the potential influence of altering MDR1 activity and its effect on therapy outcome as well as susceptibility to develop cancer.White blood cells (WBCs) isolated from 106 blood sample of acute leukemic patients were provided by Iraqi hospitals in Medical City. These samples were distributed as follows : 46 newly diagnosed patients with acute leukemia who had not received chemotherapy and follow - up 25 after 1st induction, 17 after 2nd induction and 8 at consolidation, with 10 blood samples of healthy voluntaries. Two comparative groups were taken included 46 sample of peripheral blood (PB) and 26 sample of bone marrow biopsy (BMB) in paraffin blocks to validate the level of gene expression compare to WBCs. For genotyping analysis, 56 of blood sample were taken to study genetic variation of MDR1 gene polymorphism. The samples preservation with TRIzol was done. Samples subjected to total RNA and DNA extraction, then molecular study by using reverse transcription, Quantitative Real Time - polymerase chain reaction (qRT - PCR) and direct sequencing, at Molecular Oncology Unit in Guy´s Hospital - Kings College / London.The study reached at the following results : 1 - The results showed age groups (20 - 39 years) were associated with acute myeloid leukemia (AML), while (13 - 19 years) with acute lymphoblastic leukemia (ALL).2 - The level of MDR1 gene expression showed high significant differences with WBCs compared to PB and BMB.3 - The clinical outcomes indicated that the rate of complete response (CR) of newly diagnosed acute leukemic patients was 19(41%), while 27(58.7%) was non - responder (NR).4 - Statistical analysis showed significant differences with NR at initial diagnosis in acute myeloid leukemia, while appeared after 1st induction in lymphoid type.5 - The results of positivity MDR1 gene expression were 10(21.7%) out of 46 newly diagnosed in acute leukemia, while 36(78.3%) were MDR1 - negative depend on (1.1±0.03) cutoff value.6 - The positivity MDR1 gene expression appeared mainly in non - responders patients at initial diagnosis, and with early relapse patients, after complete remission, in consolidation.7 - The MDR1 mRNA expression showed significant differences with high level in NR compared to CR patients at initial diagnosis. During treatment follow up the increased level of MDR1 gene expression in CR patients and appeared non - significantly with NR.8 - The results of MDR1 C1236T genotype and allele frequency showed that 1236CC wild type genotype and C allele were significantly frequent in healthy control. While CT heterozygous genotype frequency was highly significant in AML and no significant difference in allele frequency. ALL showed non - significant difference in genotype and allele frequency of MDR1 C1236T.9 - Odds ratio and 95% confidence interval (ORs and 95%CI) analysis showed no evidence associated with risk factor in MDR1 C1236T ALL carriers. While risk factor observed in AML with MDR1 1236CT carriers.10 - The results of MDR1 phenotype - genotype association indicate that MDR1 1236CC wild type was significantly high expression among healthy and it was aprotective genotype. While the MDR1 1236CT showed significant differences with high level of MDR1 gene expression in AML patients. Whereas ALL revealed significant differences in high level of MDR1 gene expression with MDR1 1236TT genotype. Both CT and TT were affected genotypes.11 - The results of MDR1 G2677T genotype and allele frequency indicated that 2677GA genotype significantly appeared with low frequency in healthy control with no significant difference in allele frequency. Both ALL and AML showed high significant frequency in 2677GT genotype. G allele frequency was showed significant differences in AML while non - significant in with ALL.12 - Odds ratio and 95% confidence interval (ORs and 95%CI) analysis showed the MDR1 2677GT genotype was associated with risk factor to developing ALL and AML. Whereas the GG appeared associated with AML only.13 - MDR1 phenotype - genotype association, indicate that MDR1 2677GA genotype was significantly high expression in healthy individual. While AML patients showed significant differences with high level of MDR1gene expression in 2677GT genotype. ALL showed significant differences with high level of MDR1 gene expression in MDR1 2677TT genotype.14 - The results of MDR1 C3435T genotype and allele frequency showed significant difference in genotype and allele frequency with heterozygous CT in both control and AML patients and mutant T allele. Whereas non - significant genotype and allele frequency with ALL.15 - Odds ratio and 95% confidence interval (ORs and 95%CI) analysis showed that the MDR1 3435CC genotype carriers associated with risk to developing ALL. While no risk factor associate with MDR1 C3435T variants to develop AML.16 - MDR1 phenotype - genotype association, indicate that the wild type 3435CC genotype was significantly high expression in healthy control. The MDR1 3453CT genotype showed high significance with high level of MDR1 gene expression inAML. While ALL showed significantly high level of MDR1 gene expression in 3435TT genotype.17 - The results of MDR1 genotype - phenotype association showed similar impact of MDR1C1236T, G2677T/A and C3435T genotypes in AML clinical outcomes. The MDR1 CT/GT/TT genotypes were associated in NR AML with high level of expression at presentation, compared to significant low level in CC/GG genotype. In contrast, CR patients were observed non - significant with MDR1 gene expression at presentation and significant with low MDR1CC/GG genotypes in post treatment. In regards to ALL patients the MDR1 TT genotype showed significant differences with high level of MDR1 gene expression in NR and CR ALL at presentation and significant only with NR at post treatment. So there was no clear evidence between MDR1 genotypes and clinical outcome with ALL.18 - The haplotype results showed that the three MDR1 C1236T, G2677T/A and C3435T genotype were linkage disequilibrium significantly with heterozygous haplotype B (CT - GT - CT) compared to A(CGC) and C(TTT). Also B haplotype appeared significantly with high level of MDR1 gene expression compared to A and C. According to the clinical outcome, haplotype B was observed significant differences in NR AML patients while other haplotypes were non - significant

اليات استحثاث المقاومة في نبات الخيار بواسطة Trichoderma harzianum وBacillus subtilis وGlomus mosseae اتجاه الفطر Rhizoctonia solani المسبب لمرض سقوط البادرات == Resistance Mechanisms In Cucumber Plant By Trichoderma Harzianum, Bacillus Subtilis And Glomus Mosseae Against Rhizoctonia Solani Causing Seedling Disease

اسم المؤلف: رباب مجيد عبد الذهيبي
اسم المشرف: علي هاشم الموسوي | هادي مهدي عبود
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نفذت هذه الدراسة للتحري عن اليات استحثاث المقاومة في نبات الخيار ضد الفطر الممرض Rhizoctonia solani بوساطة المعاملة بالعوامل الاحيائية وهي harzianum Trichodermaو Bacillus subtilis وGlomus mosseae وتاثير ذلك في نمو نبات الخيار وحاصله النامي تحت ظروف البيت | This study was carried out to investigate the mechanisms of induced resistance in cucumber plant against pathogenic fungus Rhizoctonia solani by bioeagent factors Tricoderma harzianum fungus, Basillus sibtilis bacteria and mycorrhizal fungus Glomus mosseae. The effect in parameters growth and yield of cucumber plant which growing under greenhouse and field conditions. The pathogenic fungus showed high Pathogenesis on cucumber plant. Three isolated of B. subtilis and T. harzianum showed high antagonism ability against R. solani under laboratory condition. For mycorrhizal fungus G. mosseae isolated was tested to study their ability in cucumber growth for chosen the best isolated which can used in anther experiment which results were as follows : - A - Pots experiment 1 - The bioeagent factors was showed their ability to induced several mechanisms of resistance in cucumber plant against pathogenic fungus R. solani, Which included increasing the activity of defense enzymes in existed of pathogenic fungus R. solani, The treatment (T. harzianum + G. mosseae) was recorded highest level in enzyme activity of Phenylalanine ammonia layase enzyme which was (25.3541) unit/gm fresh weight, While T. harzianum treatment showed significantly increased the enzyme activity of Polyphenol oxidase and Peroxidase which was (10.6707, 2.6137) unit/gm fresh weight respectively. This study showed no enzyme activity for chitinase enzyme excepted in some treatment and some periods.This study recorded increased in plant content of total phenol, lignin, salicylic acid hormone, total protein, The (G. mosseae + B. subtilis) treatment overtaken on other treatments in increased plant content of total phenol which was (1.989) mg/gm fresh weight, B. subtilis treatment was significantly increased plant content of lignin which was (18.318) mg/gm fresh weight, The treatment (T.harzianum+ B. subtilis) showed significantly increased plant content of salicylic acid hormone which was (1.8658) mg/gm fresh weight. This study recorded no Jasmonic acid hormone activity in treated plant except in some treatments but with low level, The treatment (G. mosseae + T.harzianum + B. subtilis) was recorded highest content in total protein which was (239.3) ?g/mg. 2 - The rate of seedling death and disease index was significantly reduced in bioeagent treatments, The lowest rate for seedling death in the treatment of (G. mosseae + B. subtilis), (G. mosseae + T.harzianum) and (G. mosseae + T.harzianum + B. subtilis) which were 11 % each for them comparison with control treatmentwith pathogen which was 100%.3 - The rate number of spores and percentage of mycorrhiza infection was significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. The treatment of mycorrhiza infection with pathogen recorded highest number of mycorrhizal spores which was (1410) spore. The mycorrhiza infection reach to 100% in (G. mosseae + T.harzianum) and(G. mosseae + T.harzianum + B. subtilis) treatment with pathogen fungus. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest index of mycorrhiza infection which reach to (80)%, But the index of mycorrhiza infection and mycorrhizal root weight were decreased in the existed of pathogenic fungus, Mycorrhizal dependency it was increased in all treatments with pathogenic fungus and the treatment of (G. mosseae + B. subtilis) with pathogen recorded highest percentage in Mycorrhizal dependency which was (58.873)%. 4 - The plant content of chlorophyll (a, b, total) was significantly increased, The tratment of (G. mosseae + B. subtilis) with pathogen recorded highest level in chlorophyll a it was (5.30)mg/gm, the highest level in chlorophyll b found in(G. mosseae + T.harzianum + B. subtilis) without pathogen it was (6.07)mg/gm, (G. mosseae + T.harzianum + B. subtilis) treatment without pathogen recorded highest level in total chlorophyll it was (10.79)mg/gm. The concentration of phosphor concentration increased in shoot part of (G. mosseae + T.harzianum) without pathogen which was (0.538)%, potassium concentration were decreased in all treatments with pathogen excepted (G. mosseae + T.harzianum) treatment, The highest level of potassium in treatment of (B. subtilis) without pathogen which recorded (4.65)%, For nitrogen concentration was increased in all treatments with pathogen and the highest level in (T.harzianum) which was (5.114)%. Carbon concentration significantly decreased in all treatments with pathogen and (G. mosseae + B. subtilis) without pathogen recorded highest concentration in carbon which was (46.203)%. 5 - The result showed that all growth parameters either in the absence or presence of pathogenic fungus was significantly increased in all bioagent treatments.The percentage of germination reach to (100)% in (B. subtilis) with or without pathogen , the treatment (G. mosseae + B. subtilis) with pathogen recorded highest level in shoot length it was (92.40)cm, And the same treatment recorded highest rate in fresh and dry weight for shoot part which was (7.412, 0.990) gm respectively, (T.harzianum) without pathogen recorded highest rate in leaf area it was (453.083)cm2, The highest rate of flower number was in (T.harzianum) with pathogen which was (15.66)flower/plant. The fresh and dry weight for root, root length, root surface area and the rate of root diameter were significantly increased in comparison with control treatment without pathogen. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest level in fresh and dry weight for root which were (0.359, 0.094)gm respectively. Highest root length in (G. mosseae + B. subtilis) without pathogen it was (48.14)cm, root surface area decreased in the treatments with pathogen and the treatment (G. mosseae + T.harzianum + B. subtilis) without pathogen recorded highest root surface area it was (19.16)cm 2, The root diameter was increased in(G. mosseae) with pathogen was reach to (0.363)mm.B - Field experiment 1 - Under field condition the percentage of germination increased in the treatment without pathogen which were (94.44)% in treatments (G. mosseae) , (B. subtilis) and (G. mosseae + T.harzianum) respectively, In the treatments with pathogen the percentage of germination reduced and The bioeagent factors showed no reduction in percentage of disease infection or disease index with pathogenic fungi R. solani under field condition which reach to (100)%. 2 - The rate number of spores, percentage of mycorrhiza infection, index of mycorrhiza infection, mycorrhizal root weight and Mycorrhizal dependency on cucumber plant significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. (G. mosseae + T.harzianum + B. subtilis) significantly overtaken on other treatments in increased rate of mycorrhizal spors it was (3950)spore, The mycorrhiza infection reach to (100)% in all treatments, For index of mycorrhiza infection the treatments of (G. mosseae + B. subtilis) and (G. mosseae + T.harzianum) recorded highest rate which reach to (100)%, (G. mosseae + T.harzianum + B. subtilis) treatment recorded high increased in mycorrhizal root weight and Mycorrhizal dependency which were (545.67 gm, 67.78%) respectively. 3 - The plant content of total chlorophyll was significantly increased, The highest level in(G. mosseae + T.harzianum + B. subtilis) treatment which was 5.19 mg/gm. There was significantly increased in the concentration of nutrition elements P, K, N, C in cucumber plant. The treatment (T.harzianum+ B. subtilis) recorded highest concentration in phosphor and nitrogen which were (489.4, 3.62) % respectively, The treatment of (G. mosseae + T.harzianum + B. subtilis) recorded highest concentration of potassium which was (2.975)%. The highest concentration of carbon recorded in B. subtilis treatment which was (34.823) %. 4 - All shoot, root and yield parameters of cucumber were significantly increased under field conditions. The treatment (T.harzianum+ B. subtilis) recorded significantly increased in plant height, dry shoot weight, leaf area rate number of flowers, rate number of fruit and fruit weight which were (42.87 cm, 10.85 gm, 631.06 cm2, 19, 17, 1275 gm) respectively. The treatment G. mosseae recorded significantly increased in fresh shoot weight which was (32.76) gm. The treatment with (T.harzianum+ B. subtilis) recorded significantly increased in fresh and dry weight of root, root length, root surface area, rate of root diameter, rate number of fruit and fruit weight which were (11.4 gm, 5.5 gm, 151.71 cm, 44.05 cm2, 0.763 mm, 17, 1275 gm) respectively,

دراسة مقارنة لتاثير بعض المستخلصات النباتية وبعض الاحماض الكاربوكسيلية في البكتريا المسببة لالتهابات الحروق == Comparative Study of The Effect of Some Plants Extract And Carboxylic Acids On Contaminating Bacteria In Burns Infection

اسم المؤلف: سـعاد خليـل ابراهيـم
اسم المشرف: مهدي ضمد القيسي | جنان مجيد العقيدي
الموضوع العام: علوم الحياة
السنة: 2009
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت (500) عينة من المرضى المصابين بالحروق من ثلاث مستشفيات في مدينة بغداد للمدة من 1/6/2007 ولغاية 1/11/2007. زرعت هذه المسحات على الاوساط الزرعية للعزل الاولى وتم تشخيصها بالفحوصات المظهرية والبايوكيميائية. اظهرت (489) عينة ملوثة بالبكتريا وبنسبة | • Five hundreds swabs were collected from patients suffered from burns in three hospitals of Baghdad city, for the period from 1st June 2006 to 1st November 2007. The swabs were implanted in order to be isolated and diagnosed by morphological and biochemical tests. • The results showed that 489 swabs were contaminated with bacteria (97.8%), while 11 swabs were not (2.2%). The identification and prevalence of the bacteria was as follow Pseudomonas aeruginosa was found in 148 swabs (30.27%), Klebsiella pneumoniae in 102 (20.26%), Staphylococcus aureus in 79 (16.16%), Escherichia coli in 70 (14.31%), Proteus mirabilis in 52 (10.63%) and Enterobacter cloacae in 38 (7.77%). • Information card for each patient was assigned included age, sex, time of suffering from burns, social status and address.• The degrees of suffering were as follow : - First degree of burns, 201 cases (40.2%). - Second degree, 187 cases (37.4%). - Third degree, 112 cases (22.4). Sensivity test to antibiotic and was done for all the isolated bacteria, and it was found, that, they were sensitive to Vancomycin, Ciprofloxacin, Amikacin, Tetracyclin and Fusidic acid within the percentage ratio of 0.0, 59.1, 3.7, 58.5 and 11.6%, respectively. Also it was found that the investigated bacteria showed high Vesistance to Penicillin G (87.9%), Erythromycin (93.9%), Trimethoprim (100%), Carbencillin (83.9), Cefotaxime (63.4%), Ampicillin (75.9%), Gentamycin (67.9%), Streptomycin (83.6%), Nalidix acid (89.4%) and Linomycin (83.2%). o Searching for the active compounds in the extracts of investigated plants (Linum usitatissimum, Nigella sativa and Eruca sativa) was conducted. It was found, that, the seeds of the three plants contained Glycosides, Alkalis, Flavonoids, Soapiness and Resins, which are not water soluble, but could be detected by the alcoholic extraction. Cumarin was noticed in the seeds of N. sativa and E. sativa only. o Different concentrations of water and alcohol extracts were prepared in addition to the isolation of oils, soapiness and Flavonids. The effectiveness of the extracts was tested at the concentration of 16, 32, 64, 128, 256 mg/ml for all the plants, against the bacteria.o The Phenol, water and alcohol extracts of L. usitatissimum and E. sativa showed high effect against the bacterial species, while that of N. sativa was less affective.o The Minimum inhibition concentration (MIC), was found to be different according to the type of extract and the species of bacteria.o The effects of the organic acids (Acetic, Citric, and Lactic) were tested at concentrations of 0.5, 1, 1.5, 2, 3 and 4% of each acid. An increase in the diameter of inhibition area, related with the increase in the acid concentrations, was detected. It was found that the acetic acid was more affective against the microorganisms, followed by the Citric acid and then the Lactic acid.o on the base of ratios of inhibition area diameters (in vitro), the results illustrated that E. coli was more affected by Acetic acid when the diameters reached 18.75, 21.75, 30.75, 38.25, 40.00 and 44.75 mm, respectively. The concentration of 1.5% was the effective enough against E. coli as diameters rates for P. mirabilis were 19.25, 18.25, 18.0, 22.56, 27.75 and 31.0 mm, respectively, followed by Ps. aeruginosa, S. aurues and En. cloacae when exposed to the optimum concentrations of Acetic, Citric, Lactic acids. The diameters at concentration of 4% were 23.50 and 23.66 mm. o The water extracts from all the involved plants, in addition to the organic acids showed good levels of effectiveness against the different studied bacterial species, when suffered by burns rats were exposed to them. A clear decrease in bacteria numbers in these rats in comparison with non - treated animals, in the swabs removed from the treated and non - treated rats, in addition to the absence of any histophathalogical changes in livers and skins of the treated animals. o Biochemical tests of serums from animals treated with the water extracts and organic acids at concentration of 16 mg/Kg and 18 mg/Kg for seven days and the microscope investigation, proved that these compounds were not toxic.o The effects of the water extracts and the organic acids were studied too from the point of the immunity point of view. An increase in the Levels of IgG and IgA of the supplementary systems C3 and C4 was noticed in the suffered from burns in general. The levels of IgG and IgA tended to be normal after the treatments with the water extract from the studied plants and with the organic acids.o The L D50 of N. sativa and E. sativa was 18 mg/kg while it was 16 mg/Kg of L. usitatissimum.

دراسة جزيئية عن جين المقاومة mecA في بكتريا العنقودية الذهبية المقاومة للمشيسلين والمعزولة من بعض مستشفيات بغداد == Molecular Study For Detection of Meca Gene In Methicillin - Resistant Staphylococcus Aureus Isolated From Some Hospital In Baghdad City

اسم المؤلف: لمى ياسين موسى
اسم المشرف: محمد ابراهيم نادر
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two hundred and thirty clinical specimens were collected from two different hospitals in Baghdad during the period (December 2012 to April 2013). These specimens were collected from visitors, hospitalized patients and the health care workers in these hospitals. The specimens included nasal swab, wound swab, burn swab, abscess and pus, sputum, ear swab, urine and blood culture diagnostic results show that 150 out of the (230) specimens gave positive bacterial cultures and (100) isolates are characterized as Staphylococcus aureus depending on the cultural and biochemical examinations.the coagulase test was performed and the results showed that from total 150 isolates of Staphylococci, 100 isolates (61%) were coagulase - positive (COPS), while only 50 isolates (39%) were coagulase negative (CONS). In addition, the distribution of methicillin resistance among Staphylococcus spp. was investigated.The use of the antibiotic susceptibility profile for these isolates was examined against methicillin resistance. Using disk diffusion method revealed that (13) isolates were proved to be methicillin resistant Staphylococcus aureus (MRSA), While (87) isolates of S. aureus showed sensitivity to methicillin (MSSA) and there was no intermediate resistance among these isolates.The ability of MRSA isolates to produce some virulence factors were investigated and the results showed that MRSA isolates produce many enzymes and toxins that contributed in their virulence such as protease, urease, dnase and gelatinase, and also produce a beta types of haemolysins.The ability to produce slime layer by MRSA isolates was also investigated and the results showed that all isolates of MRSA were produced slime layer when theytested by Congo red agar method and the results showed that all of MRSA isolates produced strong slime layer.One of the aims of this study was to adopted a accurate diagnostic method to detect S.aureus by its genetic material contents through extracting of DNA and gel electrophoresis of the PCR product for the specific gene.Detection of methicilline - resistance gene represented by A confirmatory test was carried out for the selected isolates using Polymerase chain reaction (PCR) technique for further characterization up to the species level by the amplification of (mecA) gene.This is Staphylococcus aureus specific gene that encodes the extra Penicillin Binding Protein, which is unique to methicillin - resistant staphylococci. All the (13) positive isolates by disk diffusion test are found to be positive for the presence of (mecA) gene as their agarose gel revealed the presence of DNA band of mecA gene with a molecular size about (200 bp.).Results of the detecting (femB) gene showed that it was positive in all of MRSA isolates as they appear to have a band with a molecular size of about (651 bp). The genetic determinants of methicillin resistance mecA and femB genes were amplified using multiplex PCR technique in order to identify methicillin resistant (mecA+) and susceptible (lacking mecA) staphylococci and to differentiate S. aureus (femB+) from coagulase negative staphylococci (lacking femB). All of the S. aureus isolates (100%) were found to harbor femA gene, it is species specific marker for S. aureus.

استجابة ثلاثة اصـناف من حنطة الخبز Triticum aestivum L. باضافة سماد NPKZn والرش بالجبرلين == Response of Three Bread Wheat (Triticum Aestivum L.) 3With Gasprying Fertilizer And NPKZn Cultivars To

اسم المؤلف: عبد الجبار جاسم جريان
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A field study was conducted at the farm of Biological Dpartment, College of Education (Ibn Al - Haitham) University of Baghdad during the grown season 2013 - 2014, to study the effect of compound fertilizer NPKZn (15 : 15 : 15 : 1) - Turkish origin was introduced to Iraq recently - in both ) 3(GA acid Gibberellicvarious concentrations of with morphological and chemical indicators, the components of yield, some quality and biological characteristics of three varieties of the Iraqi wheat (Bohoth 22, Alfath, Bohoth158). Experiment was carried out according to the design of complete sectors of randomization [Randomized Complete Block Design (RCBD)].160) (0 of fertilizer, two levels used experiment Asthree replicates, with ) 015, 100, acid concentrations (25, 50 Gibberellicof fourand 1 - .hKg and three varieties of as well as to the treatment of comparison 1 - .lmg wheat. So the expriement was factorial on (2 x 5 x 3).It included 90 experimental units..The study of components of yield have gone on its : 1. There is difference in these three different varieties Bohoth 22, Alfath, and Bohoth 158 in the studied indicators.The class Bohoth 158 exceeds on the other categories in the following phenotypic indicators (dry weight of vegetative part, space of flag leaf and sustainability of biomass), with increase as like {(17.85, 11.37), (14.66, 7.33) and (17.02, 14.24)}%, respectively, while Bohoth 158 significantly exceeds on the other varieties absolute growth rate. And also the differences were significant between varieties in the following chemical indicators (phosphorus and calcium content and the percentage of protein in the grain) and in the components of yield there was significant increase in the (spike length with awn and without its, the number of spikes, weight of spike and its grains, weight of grains in spike and grains yield), while in the Bohoth 158 there was a significant increase in qualitative and rheological characteristics for the following qualities, (the proportion of wet and dry Glutine, flour absorption susceptibility of water, sedimentation values, ash content in coarse bran compared to others two classes. 2. The results of the study showed that the transactions under the influence of fertilizer has excelled significantly in all the study indicators (morphological and chemical components of yield, quality and rheological) with the exception of the dry weight of roots. acid was the best among Gibberellic 1 - .lmgof 100 concentration 3. The the concentrations giving him the highest average of indicators of morphological and physiological components of yield, quality and rheological. and spray 1 - 160 Kg.hbetween the manure level overlap 4. The result Gibberelllic acid showed 1 - mg.l 100 of concentration under the significant increase in all studied indicators except for the (dry weight of roots, the sustainability of biomass, and 1000 - grain weight) 5.The trio overlap transactions showed exceeding of Bohoth 158 nder the influence u 1 - .hKg160 in level fertilizer of add ithw significantly 22 Bohothcategories : other acid onGibberellic of 1 - .lmgof spraying 100 and Alfath in the following indicators : Dry weightof the flag leaf, spike length with awn, number of spikes, grains yield, the percentage of dry Glutine and the values of sedimentation.

دراسة بيئية للدايتومات الملتصقة على بعض النباتات المائية في هور العودة ضمن محافظة ميسان جنوب العراق == A Study On The Ecology of Epiphytic Diatom On Some Aquatic Plants In Al - Auda Marshes / Maysan Province / Southern Iraq

اسم المؤلف: الاء عيسى موسى البوعجي
اسم المشرف: جنان شاوي الحساني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الفصلية على الدايتومات الملتصقة على النباتات المائية لثلاث مواقع في هور العودة ضمن محافظة ميسان جنوب العراق (من شهر تشرين الاول 2013 لغاية شهر تموز 2014) نظرا لاهمية الدايتومات في الانظمة البيئية المائية والاهمية البيئية لاهوار العراق على مس | Seasonal study on the quality and quantity of epiphytic Diatoms was conducted in the Al - Auda marsh within Maysan Province throughout one year from October 2013 to July 2014.These Diatoms have an important role in biotic ecosystems. Three taxa of aquatic plants were selected (Phragmites australis Trin ex stand, Ceratophyllum demersum L. and Typha domengensis Pers) in three sites within Al - Auda Marsh; these sites were Al - Adleh, Al - Auda and Um - Almashahef. Also, the study included measuring physical and chemical factors of all the study sites, such as : temperature (air and water), power of hydrogen (pH), total hardness(TH), Calcium (Ca), Magnesium (Mg), turbidity (Tur.), light penetration(LP), depth of water (D), total dissolved suspend material (TDS), total suspended solids (TSS), total alkalinity (TA), dissolved oxygen (DO), total nitrogen (TN) and total phosphorus (TP) and reactive Silicate (SiO3). The mean ranges of the studied factors were recorded : air temperature (10 - 38 C°), water temperature (13 - 34 C°), pH (7.1 - 8.6), TH (672 - 3800mg/l), Ca (97.8 - 264.5 mg/l), Mg (125.1 - 896.13mg/l), Tur.(7.62 - 30.7NTU), LP(46 - 113cm), D (190 - 413cm), TDS (1170 - 3075 mg/l), TSS (0.02 - 0.08 mg/l). while TA (207.5 - 300mg/l), DO (6.4 - 13.5 mg/l), TN (2.41 - 8.58µg/l), TP (0.013 - 0.23 µg/l) and SiO3(101 - 776.77 µg/l).Results of the study indicated presence of 111 taxa of epiphytic diatoms, which belonged to 13 families and 26 genus (one family and two genus of centric diatoms, 12 families and 26 genus of pennate diatoms).The present study recorded 21 species that were found only on one host plant, 20 species that were found on two host plants and 70 species that were found on all host plants, nine of diatoms taxa were present in all host plants among all study seasons which are Achnanthes minutissima, Cocconeis placentula var. euglypta, Cyclotella meneghiniana, Synedra affinis, Syneda ulna , Gomphonem parvulum, Mastogloia smithii var. amphicephala, E. zebra var. porcellus, Rhopalodia gibba.One of the diatoms taxa was recorded in the present study is new to Iraqi flora, the new record of diatom include one taxa of Achnanthidiaceae family (Achnanthes exigua var. constricta). The results of this study included description of the basic characteristics of this taxa with complete photographic documentation.It was evident, from the results, that the morphological form of host plants influenced the number of the attached epiphytic diatoms. C. demersum had attracted 92 diatoms taxa(35%) while the lowest number was 82 taxa (31%) attached by Typha domengensis. The seasonal variation of diatoms growth was evident. Spring 2014 was characterized by the highest number of attached taxa (69 on P. australis - 11%) whereas the lowest number was observed on C. demersum (32 taxa - 5.4%) in autumn 2013.Numbers of epiphytic diatoms fluctuated among study sites, seasons and aquatic plants. The total number of epiphytic diatoms on P. australis ranged between 0.73×104 cell/gm wet weight in summer 2014 at Al - Auda and 406.89104 cell/gm wet weight in spring 2014 at Um - Almashahef. C. demersum recorded highest total number 513.9 ×104 cell/gm wet weight in winter2014 at Al - Adleh, while the lowest total number was 0.1×104 cell/gm of wet weight was recorded in the spring 2014 at Um - Almashahef. While total number of taxa on T. domengensis ranged (0.84 - 427.89) ×104 cell/gm wet weight in summer and winter 2014 at Um - Almashahef.Fluctuations in the total number of main epiphytic diatoms families were observed on all aquatic host plants throughout the study period. The higher total number(92.59×104 cell/gm of wet weight) of diatoms recorded in Achnanthidiaceae family in summer 2014 on P. australis and lower total number (0.28×104 cell/gm of wet weight) on same plant observed in Rhoicospheniaceae family in spring 2014. C. demersum showed highest total number (95.15 ×104 cell/gm of wet weight) in Fragilariaceae family in spring2014, while the lower total number (0.3×104 cell/gm of wet weight) was in family Rhoicospheniaceae in autumn 2013. Individual of Achnanthidiaceae family were recorded as highest total number (168.57×104 cell/gm of wet weight) on T. domengensis in Achnanthidiaceae family in the winter of 2014, but lower total number (0.28×104 cell/gm of wet weight) recorded in Coscinodiscaceae family in the same season. The study showed highest values for richness index (3.77) in the spring of 2014 for epiphytic diatom on C. demersum in Al - Auda site, while lower richness index recorded was (0.6) on T. domengensis in theautumn of 2013 at Um - Almashahef. Shannon - Weaver index ranged (0.24 - 2.45) for epiphytic diatom on P. australis during winter of 2014 at Um - Almashahef and autamn 2013 at Al - Auda, respectively. Highest values for Evenness index were recorded (0.28) for epiphytic diatom on C. demersum in the spring of 2014 at Um - Almashahef site, while for the same site lower values recorded was (0.03) on T. domengensis in winter 2014. Higher percentage for Jaccard similarity index (63.2%) obtained between epiphytic diatoms hosted on T. domengensis and lower percentage of similarity (28.9%) found among the common taxa of epiphytic diatoms on C. demersum, and highest percentage for Jaccard similarity index between host plants was (70%) between P. australis and C. demersum while lower percentage of similarity(67%) was obtained between T. domengensis and C. demersum.

دور الكاينيتين والسماد المركب (NPKZn) في تحمل نبات الفلفل Capsicum annuum L.)) للاجهاد الملحي == Role of The Kinetin And NPKZn In Salt Stress Tolerance of Sweet Pepper (Capsicum Annuum L.)

اسم المؤلف: سعاد عبد سيد الجلالي
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجربة اصص في الحديقة النباتية التابعة لقسم علوم الحياة، كلية التربية للعلوم الصرفة - ابن الهيثم، جامعة بغداد في الموسم الربيعي عام 2014 هدف الدراسة كان دراسة تاثير تركيزين من كلوريد الصوديوم هي 50) و100) مليمول.لتر - 1 فضلا عن معاملة السيطرة واربعة | The aim of the pots experiment which was conducted in the Botanical Garden of Biology Department in the Faculty of Education /Ibn - Al - Haytham / University of Baghdad, during the spring season of 2014 was the influence of different concentrations of Sodium chloride (50, 100) mM.L - 1 in addition to the control treatment and and Four concentrations of kinetin (25, 50, 75, 100) ppm in addition to the control treatment.It is also aiming to study the effect of application and non application the level 160 Kg.H - 1of NPKZn fertilizer, and their interactions in some morphological characteristics root length, root size, plant height, the internode length, stem diameter, Leaf area, dry weight for root and shoot and the absolute growth rate for shoot.and some physiological characteristics Elements content and protein ratio in root, Elements content, protein ratio, total chlorophyll content, carbohydrates percentage and cytokinine concentration in shoot. Biochemical characteristics (enzyme activity of the enzymes) (SOD, POD and CAT) and some antioxidants that nonenzymatic : (the proline and vitamin C concentration) in shoot. Malondialdehyde (MDA) content in shoot, and some Floral morphological, physiological and biochemical characteristics of fruits (Number of branches, The total number of flowers, Number of blooming flowers, ratio of aborted Flowers, Number of knotted flowers, the number and size of fruits, their wet weight , nitrogen and calcium concentration, protein percentage and vitamin C concentration in fruits of pepper plant California weleander variety.The experiment was designed according to Randomized Completely Block Design (RCBD)with three facters kinetin, manure addition and Sodium chloride,) consisting 90 pots. Seedling of pepper plant was planted on 4/2/2014 acompanied by making all agricultural operations of irrigation and the removal of the jungles. results were obtained can be summarized as follows : 1 - The results showed that increasing the concentration of sodium chloride from zero to 100 mM.L - 1 Led to a decline in morphological, physiological and floral characteristics, with and significant increases in the content average of sodium, chloride, The proportion of flowers aborted and biochemical characteristics. The rate quality effectiveness for enzyme SOD increased from 7.24 to 34.66, POD from 8.10 to 26.35 and CAT from 4.91to 39.57 (unite mg protein - 1) , and proline acid content from 23.05 to 40.30 (mg.L - 1) and vitamin C concentration from 2.67 to 3.68 % and Malondialdihyde (MDA) from 1.84 to 3.86 (µM.gm F.W. - 1)2 - As concerns kinetine treatment effect, the results showed a significant increase in the average of morphological, physiological and floral characteristics, and significant decreases in the average of sodium, chloride contents and biochemical characteristics, in the cased increasing in the concentration of kinetine from 0 to 100 ppm caused decreases in the average of quality effectiveness for enzyme SOD from 25.79 to 21.91, POD from 21.83 to 17.20 and CAT from 27.88 to 21.73 (unit mg protein - 1) , and proline acid content from 34.27 to 31.68 (mg.L - 1), Malondialdihyde (MDA) from 3.15 to 2.85 (µM.gm F.W. - 1) and vitamin C concentration from 3.42 to 3.23 %.3 - application the level 160 Kg.H - 1of NPKZn Manure caused a significant increases in all averages morphological, physiological and floral characteristics, and significant decreases in the average of sodium and chloride contents and biochemical characteristics, fertilizing with the level 160 Kg.H - 1of NPKZn fertilizer caused decreases in the average of quality effectiveness for enzyme SOD from 22.03 to 19.88, POD from 17.70 to 14.53 and CAT from 23.34 to 17.34 (unite mg protein - 1) , and proline acid content from 32.84 to 28.93 (mg.L - 1), Malondialdihyde (MDA) from 2.97 to 2. 75 (µM.gm F.W. - 1) and vitamin C concentration from 3.29 to 3.03 %.4 - Results of the bilateral interpenetration between the Fertilization by fertilizer NPKZn and Sodium chloride concentrations, showed that the fertilizations has a positive and effective influence in reducing the negative effects of sodium chloride in all characteristics studied. 5 - The results showed the positive role of kinetine in decreasing the negative effects of sodium chloride Through bilateral overlap between factors. Especially when the concentration of 75 ppm of kinetine and concentration 100 of sodium chloride In all morphological, physiological and biochemical floral characteristics of plant.6 - The results indicated that the Interference between the fertilizer NPKZn and kinetine has a positive and clear role to both factors increase morphological, physiological, floral characteristics and reduce biochemical characteristics.7 - The effect of the triple overlap between study factors was significant, The results showed That plants sprayed kinetine And fertilization led to a significant reduction of most of the negative effects resulting from the of high concentrations of Sodium chloride in all morphological, physiological and flowering traits. Especially in the concentrations 75 ppm kinetine and in the existence of fertilization.

استخدام الزراعة النسيجية والفطر Glomusmossaes في الازالة الحيوية لعنصري الرصاص والكادميوم وتحمل الملوحة لنبات السيسبان Sesbaniarostrata L == Application of Plant Tissue Culture And Glomusmossaes In Bioremediation of Lead, Cadimum And Salinity Using Sesbaniarostrata L. Plant

اسم المؤلف: التفات فاضل شحاذة الطائي
اسم المشرف: علي هاشم الموسوي | كاظم محمد ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجارب عدة لغرض دراسة تحمل نبات السيسبان Sesbania rostrata L. للكادميوم والرصاص وكلوريد الصوديوم على مستوى النبات الكامل ومزارعة النسيجية. استحث الكالس واديم على وسط موراشيج وسكوج 1962 والمجهز 0.3 ملغم/لتر من الكاينتين و2 ملغم/لتر من حامض 2, 4 داي مث | Several experiments were carried out to study cadmium, lead and sodium chloride tolerance at the tissue culture or whole plant levels of Sesbania rostrata. Callus was induced and maintained on Murashige and Skooge (1962) medium (MS) supplemented with 0.3 mg/l Kinetin and 2 mg/l 2, 4 - dichlorophenoxy acetic acid (2, 4 - D) using cotyledons as the source for callus induction. Different concentrations of cadmium, lead and sodium chloride directly or gradually were added to the culture medium as contaminants. Selected tolerant cell lines were subjected to regeneration. Callus showed better tolerance to gradual exposure than direct addition. The concentrations of Potassium, lead, Cadmium and Sodium recorded to 17, 24, 27, 35 ppm when 1.0, 2.0 mg/L and 0.4% of cadmium, lead and sodium chloride were added respectively. The study included the effect of the cytokinin (benzyl adenine, BA) and the auxin (Naphthalene acetic acid, NAA) on the regeneration of shoots from callus tolerant to pollutants reached 76, 87 and 85% for callus tolerant to Cadmium, Lead and sodium chloride respectively, when the combination of 0.7 mg/l BA and 0.2 mg /l NAA was supplemented to the medium. The effect of indole butyric acid (IBA) on rooting of shoots. The concentration 0.7 mg /l gave highest percentage of rooting amounted to 66, 70 and 85% in a medium supplemented with cadmium, lead and sodium chloride respectively. Acclimatization of plantlets using river sand soil recorded 85% survival. The study was also included effects on plant height and fresh weights, shoot fresh weight was superior at the concentration 1.0 and 2.0 mg/l of cd and pb recording 8.7 and 7.5 g respectively. Treatment with NaCl led to increase the root fresh weight recording 19.1 g. Maximum cd accumulation reached 12.5 ppm in shoots, 10.5 ppm for lead in roots, 39 ppm for NaCl in roots. The experimental work investigated the effect of mycorrhiza (Glomus mosseae) on cd, pb and NaCl removal by Sesbania plant when grown in either soil contaminated with cd, pb and NaCl or non - contaminated soil (control). Inoculation with mycorrhiza led to an increase in seed germination percentage (96%), plant height (28 cm) in non - contaminated soil, while achieved an increase in shoot and root fresh weight recording 10.0 and 6.8 g respectively. Inoculation with mycorrhiza also led to a significant increase in cd and pb accumulation in roots reached 9.0 and 87.4 ppm respectively. Meanwhile, Mycorrhiza caused a significant increase in Na accumulation in root system (42.5 ppm) which already affected K concentration as a result of competition with Na recording 16.5 ppm in shoots. Mycorrhiza inoculation led to a significant increase in number of root nodes in non - contaminated soil recording 86%. Finally, mycorrhiza succeded in infecting Sesbania rostrata roots reached 90% in non - contaminated soil while it was 85% in contaminated soil

دراسة وراثية جزيئية لبكتريا المكورات المعوية البرازية Enterococcus faecalis المعزولة من جذور الاسنان الملتهبة للانسان في بغداد == Molecular Genetics Study of Enterococcus Faecalis Isolated From Root Canal Infection of Human In Bagdad

اسم المؤلف: سوزان علي كاظم
اسم المشرف: عذراء حميد حسون
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم التحري عن نسبة وجود بكتريا المكورات المعوية البرازية Enterococcus faecalis في (100) عينة معزولة من اشخاص مصابين بالتهاب قناة جذر السن, اذ تم جمع (70) عينة من الاصابات الابتدائية لقناة الجذر، و(30) عينة من الاصابات الثانوية لقناة الجذر (اعادة العلاج) وم | To detection Enterococcus faecalis in (100) root canal sample were collected from primary and secondary root canal infection patient`s from all the ages (10 - 50) during the period of (August 2013) till (January 2014). Detection depending on cultural & microscobial characteristics of bacterial cell was done to find (45) 0f E. faecalis species & Biochemical tests & Serological diagnosis by Lancefield method done to find (24) isolates of this species & Diagnosis by Vitek2 was done to find (20) isolates of E. faecalis. When the Molecular genetics Diagnosis was done the result showed find (32) isolates belong to E. faecalis. The antibiotic sensitivity test was done by using (14) antibiotics, (5) isolates showed resistant against all antibiotics & the isolates showed multiresistant against for some antibiotics. All the isolates were resistant by (100 %) against (5) antibiotics. To detection of the isolates ability of production of protease enzyme, lipase enzyme, hemolysin enzyme & gelatinase enzyme.The results showed that (24 isolate) (75 %) were protease producer, & (8 isolates) (25 %) were lipase producer, & (16 isolate) (50 %) were hemolysin producer, & (5 isolates) (15.6 %) were gelatin producer. To detection the presence efa A gene of the isolates by used specific primer to this gene, and all isolates

دراسة تصنيفية مظهرية خارجية لبعض انواع عائلة الفراشات فرشاتية الاقدام من رتبة حرشفية الاجنحة (Lepidoptera : Nymphalidae) في وسط وشمال العراق == Morphological Taxonomic Study of Some Species of Brush Footed Butterflies (Lepidoptera : Nymphalidae) In Mid And North of Iraq

اسم المؤلف: ايناس صادق عبد الكريم
اسم المشرف: عماد احمد محمود | حسن سعيد الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study comprises taxonomic morphological study of seven species belonging to four genera of the subfamiliesNymphalinae, Danainae, Satyrinae belong to the family Nymphalidae of the order Lepidoptera.The following species were studied in the subject are : Famliy : Nymphalidae Swainson, 1827 - Subfamily : Nymphalinae Swainson, 1827Genus : Vanessa Fabricius, 1807Vanessa japonica Linnaeus, 1758Vanessa cardui Linnaeus, 1758Vanessa atalanta Linnaeus, 1758Genus : Junonia Hubner, 1819Junonia orythia Linnaeus, 1758 - Subfamily : Danainae Swainson, 1827Genus : Danaius Kluk, 1802Danaius chrysippusLinnaeus, 1758 - Subfamily : Satyrinae Boisduval, 1833Genus : Coenonmphyma Hubner, 1819Coenonmphyma pamphilus Linnaeus, 1758 Coenonmphyma spThe species Vanessa japonicaLinnaeus added as new record to the Iraq insect fauna of Lepidopteraand the species Coenonmphyma sp added as new expected species to the world.

العلاقة بين انتاج الانزيم المحلل للكولاجين وتكوين الغشاء الحياتي بوساطة بكتريا Pseudomonas aeruginosae == The Relationship Between Collagenase Production And Biofilm Formation By Pseudomonas Aeuroginosa

اسم المؤلف: امال عزيز كريم السعدي
اسم المشرف: شذى سلمان الطحان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A total of 359 samples divided as 228 clinical and 131 non clinical specimens were collected during 2012 from four hospitals in Baghdad city including : Al - Kadhymia Teaching hospital, Baghdad Teaching hospital, The Burn Specialist Hospital and Al - Imam Ali hospital, for isolation of P.aeruginosa to study the correlation between collagenase production and biofilm formation. Eighty two Pseudomonas isolates were screened for biofilm formation, 28 isolates were classified as strong biofilm formers, 25 as moderate and 27 as weak biofilm former. The 28 isolates were identifid by VITEk - 2 Compact system which confirmed that the isolates were P.aeruginosa. Collagenase production assay was used to screen 28 isolates that were strong biofilm formers inorder to detect the ability of these isolates to produce collagenase, the substrate of collagenase (collagen) was purified localy from bovin tendon and the results showed that just 8 isolates could grow in mineral salt media with collagen after 4 days of incubation. The factors affecting biofilm formation and collagenase production were studied to determine the optimual conditions for their production, those factors included : 1 - Nitrogen sources represented higher influence on collagenase production specialy (yeast extract) in media containing collagen than other media without collagen as a substrate. The specific activity differed between the 8 isolates, biofilm formation also became more pronounced with (yeast extract), while NH4Cl and NaNO3 depressed biofilm formation at the same conditions. The statistical analysis between the two parameters (biofilm and collagenase) according to different nitrogen sources demonstrated highly significance at p?0.01 with yeast extract and casein. 2 - pH, results showed that the best pH for production was 7 for both collagenase and biofilm.The statistical analysis for determination the relationshipe between the two parameters showed highly significance at p ?0.01 for different pH. 3 - The maximum production of the two parameters was at 35?C temperature which gave highly significance at p?0.01 with defferent temperature. 4 - Long incubation periods revealed increasing in collagenase production and biofilm formation which represented highly significance detween them when incubation periods were prolonged at p?0.01. Results of this study showed that collagenase production increases when bacteria switch from a planktonic to biofilm phenotype. This indicates that biofilms and collagenase are more virulent and have a greater ability to cause tissue destruction. The REP - PCR analysis using BOX - primer, showed a clusters genetic relatedness among the isolates. The isolates were grouped according to the REP - PCR in 9 different genotypes, named cluster 1 to 3 which included C1, C2, C3 with relatedness : 8 (80%), 8 (86%), 3 (80%) respectively. A19 and A20 both of them were not included in any cluster, they have 78% similarity.The REP - PCR analysis showed that the genotypic relatedness is consistently high between the 8 producer isolates and non producer isolates (13), showed similarity reached 86

عزل وتشخيص الجين lipA المنتج من بكتريا الزائفة الزنجارية من مياه الصرف الصناعي == Isolation And Identification of lipA Gene Producing Pseudomonas Aeruginosa From Industrial Wastewater

اسم المؤلف: انتصار طه لفتة
اسم المشرف: واثق عباس الدراغي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: توضح هذه الدراسة عزل lipA gene من الزوائف الزنجارية من مياه الصرف الصناعية للزيوت النباتية وتشخيصها اعتمادا على تفاعل البلمرة المتسلسل (PCR). استغرقت هذه الدراسة تسعة اشهر, من شهر شرين الاول 2014 لغاية حزيران 2015. تم جمع خمسين عينة من مياه الصرف الصناع | This study clarify the isolation and identification of lipA gene in Pseudomonas aeruginosa from industerial wastewater of vegetable oils factories depending on the polymerase chain reaction (PCR) technique. The present study had taken nine monthes starting from October 2014, till the mid of June 2015. Fifty samples of industrial wastewater were collected from the factories of the general company of vegetable oils, fourty from AL Rasheed factory and ten from AL ameen factory, the samples collected from the physical, chemical and biological treatment units, and other different wastewater tanks departments. While the four sewage samples were collected from sewer service Baghdad /Alrustumaiya. For Identification of Pseudomonas aeruginosa, two types of tests were used in this study. The first type was the routine tests, which include selective cultures, bacteriological and biochemical tests. Thirty four of fifty samples (68%) gave a positive growth and results for tests that were used to confirm the presence of P. aeruginosa. In addition to diagnosis P. aeruginosa in sewage sample which prepare the biological treatment units in vegetable oils factories as active sludge, other bacterial types have been diagnosed by using traditional methods and API 20 E system. For detection of bacterial lipolytic activity, two methods were used for this purpose, the first was the screening of bacterial lipolytic activity which was based on values of clear zones diameter around bacterial colony. The best values were between (1.9 _ 2.7) cm. The second test was carried by the Gas - Chromatography - Flame ionization detector, fatty acids solutions that were produced from hydrolysis by lipase enzyme were extracted by Petrolium ether solvent then analyzed by gas chromatograph apparatus. The second type of test was the Molecular diagnosis by using the Polymerase Chain Reaction (PCR) to detect lipA gene in Pseudomonas aeruginosa by two primers. Through two primers used, lipA 948 was the best and more specialized primer to isolation lipA gene of P. aeruginosa. It gave (100%) a positive result. While the second primer lipA 558, gave (66.66%) a positive result and that may be due to the design of this oligonucleotides was not specific for lipA of P.aeruginosa. This is so as was based on highly preserved region of 12 bacterial lipA - homologous genes for many genus and many species belong to Pseudomonas. DNA sequencing done for amplicon generated using lipA 948, this sequence aligned by using BLASTn software against NCBI database to validate the results and investigate the similarity degree with other corresponding strains.

الاكثار الخضري لنبات Spilanthes acmella (L.) Murr. خارج الجسم الحي == In Vitro Vegetative Propagation of Spilanthes Acmella (L.) Murr

اسم المؤلف: انسام زهير جاسم الحسني
اسم المشرف: بشرى محمد جابر علوش
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: وظفت تقانة زراعة الانسجة لاكثار نبات Spilanthes acmella (L.) Murr. كونه نباتا غير مستزرع في العراق ولاهميته الطبية والزراعية بوصفه نبات زينة فضلا عن استعمالاته الاخرى. عقمت البذور بمادة هايبوكلورات الصوديوم(NaOCl) وزرعت على وسط Murashige وSkoog (1962) (MS | The technique of plant tissue culture has been manipulated to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized by sodium hypochlorite (NaOCl) and cultuared on full strength Murashige and Skoog medium(1962) (MS). Nodal segments, apical shoots, and leaflets explants were taken from seedlings and cultured on (MS) medium enriched with different concentrations of Indol acetic acid (IAA), Naphthalene acetic acid (NAA) Benzyl adenine (BA), and 6 - furfurylaminopurine (Kin.). The study include the effect of node orientation vertically and horizontally on induction of shoots formation. The effect of the interaction between IAA and BA, IAA and Kin. on shoot multiplication was investigated. Rooting was also studied after inclusion of IBA and IAA to full and half - strength (MS) medium. At acclimatization stage, different ratios of river sand and peat moss as agricultural media were tested and plantlets survival was recorded. DNA isolation was carried out from fresh and dry plantlet leaves as well as from seeds and Agarose Gel Electrophoresis (AGE) was done. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. The nodes cultured vertically exhibited relatively highest response as compared with apical shoots and leaflets culture. Supplying the culture medium with 1 mg/L. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding IAA at 0.1 and Kin. at 0.5, 1.0 or 1.5 mg/L. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 14.20, 13.60 shoots/ explants with a mean length of 3.40, 2.60, 1.40 cm, respectively. Adding 0.1, 0.5, 1.0 mg/L IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots, respectively, with mean length of 3.25, 3.80, 3.80 cm, respectively. Plantlets were acclimatized successfully achieving 100% survival after four weeks when transferred to agricultural medium consisted of river sand and peat moss at 1 : 1 v/v ratio. This study showed the ability of in vitro micropropagation of Spilanthes acmella (L.) Murr. DNA isolation from drying leaves was butter than fresh leaves with the precense of liquid nitrogen.

تاثير الكلوتاثيون وبيروكسيد الهيدروجين وتداخلهما في بعض الصفات النوعية والكمية لنبات الماش Vigna radiat == Effect of Glutathione And Hydrogen Peroxide And Their Interactions On Some of The Quantity And Quality Characteristic of (Mung Bean) Plant Vigna Radiata L.

اسم المؤلف: ايمان حسين هادي الحياني
اسم المشرف: وفاق امجد القيسي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments were conducted during the spring and autum growth season of Vigna radiata L. in the year 2014 at botanical garden of Biology Department, Collage of Education for Pure Science (Ibn AL_Haithem) University of Baghdad.The experiments aimed to study the effect of glutathione and hydrogen peroxide, and their interactions on some of quantity and quality characteristic of plant Vigna radiate L.The treatment of glutathione (0, 25, 50, 75, 100) mg.L - 1 While the H2O2 (0, 5, 10, 15) ml mol. L - 1.The two experiment were designed as Randomized Complete Block Design as factorial experiment with two factors with three replication which included 60 experiment units the area of each one (1×1`) m. Results were analyzed Statistically and compared using average L.S.D at 0.05. The results of the experiments showed that glutathione revealed significantly increased all study parameter espically in 100 mg.L - 1 concentration, stem diameter by 54.56%, 28.04%, leaves number 69.34%, 53.14% for two seasons, fresh weight 44.20% for first season, the dry weight 37.43%, 91.46%, leaf area 61.61%, 151.23%.leaf area index 86.61%, 146.35%, biomass duration 60.48%, 101.06%, absolute growth rate (AGR) 32%, 92.68%, inflorescences number 56.55%, 39.90%, flower`s number 71.97%, 22.60%, root length19.82. %, 26.20%, root dry weight 76.10%, 79.25%, total function of SOD 69.32%, 40.52%, total Function of POD 29.45%, 82.25%, total Function of GPX 30.90 %, 63.62%, chlorophyll concentration of a 73.48%91.40%, chlorophyll concentration of b 35.42%, 17.67% for two seasons respectively. The total chlorophyll content 13.69%, for second season, Caroten concentration 207%, 309%, proline concentration 84.47%, 31.75%, (MDA) concentration 6.25%, 38.35%, glutathione. concentration 41.49%, 23.62 %, H2O2 concentration 52.16%, 33.24%, pods number 17.43%, 16.93%, 100 seeds weight 22.95 % 22.48%, , 93.62%, seeds yield 52.17%, 43.70%, the carbohydrate percentage 64.07%, 19.21 %, the protein percentage 22.32% for first season while the results of effect of soaking seeds with H2O2 specially with 15m ml.L - 1 concentration increased stem diameter 45.52%, 34.00%, leaves number 18.55%, 30.75%, Lateral branch 26.06%, 48.04% for Two seasons respectially, fresh weight 35.45% for first season, dry weight 40.89%, 43.85%, leaf area 53.24%, 64.53%, leaf area index 92.30%, 62.39%, biomass duration 35.35%, 57.53%, abosult growth rate34.78 %, 48%, in, inflorescences number 34.98%, 38.10% flowers number 2.987, 20.13%, root length19.44 %, 19.36%, root dry weight 99%, 96.52% total functhion of (SOD)100%, 2853%, total function of (POD) 176.57%, 40.58%, total function of (CAT)118.29%, 71.78%total functhion of (GPX)12.80%, 61.40%, Caroten concentration 54%for second season, prolineconcentration 40.93%for first season, (MDA) content 17.31% for second season, glutathione concentration, 13.68% 24.29%, H2O2 concentration 26.53%, 30.58%, number of pods 42.77%, 20.51%, seads number /pod 22.93%, 22.93%, 100 seeds Wieght 24.07%, 24.86%, seeds yield 16.69% for first season, the solubly carbohydraty percentage 43.26%for first season, the protein percentage 11.50%, 25.18 % for two seasons respicatlly, while chlorophyll a decreas concentration ed by 15m ml.L1 12.87%for second season, total chlorophyll concentration 39.66%, 26.22 for two season respicatlly. There were high significant interaction between glutathione and H2O2 in all study parameter characters in the two experiments.
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