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عزل وتشخيص بعض انواع البكتريا المكسرة للهيدروكاربونات النفطية الاليفاتية في الترسبات النفطية لمحافظة البصرة == Isolation and Identification of some species of degradable Bacteria of N - Alkanes hydrocarbons compounds in oil sludge at Basrah Governorate

اسم المؤلف: استبرق عبد الحسين سلمان جدي البوغازي
اسم المشرف: ميثم ايوب عبد القادر الحمداني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In the oil industries, large amounts of oil sludge are generated through the refining of crude oil. The main reason for its composition that is when exporting crude oil various kinds of oils (heavy and light ones) are mixed together for the purpose of exportation. But , the mixing process between different types cannot be fully , due to the lack of homogeneity among different quality of crude oil . So, the heavy parts of oil are precipitated to the bottom of tank and with passage of time they accumulate to be sediments that’s known as oil sludge stated at the bottom of the oil storage tank in the cleaning process , oil tanks are emptied and discharged in some spaces or reservoir (pits) located near the tanks called "oil pits". Which are places of dump excess oil of various types of oil from different oil tanks or they are close places from oil / water separating unites .From these spots, samples of two kinds of oil sludge : the bottom of oil tank (during the maintenance of the tank) and oil pits (near the unities of separating water from oil) are collected. Also, samples of soil surrounding the oil pits from five oil locations in Basrah city are collected too. Those locations are : west of Gurna, Northern Rumela, Southern Rumela, Nahar Umar, Alzubeir.At the rate of redundantly for each location and for each type of the study samples.This investigation is considered as the first which conducted in Iraq which include firstly the isolation and identification different types of bacterial species from different oil sludge, and secondly use some of them in biodegradation studies for two kinds of carbon sources of oil sludge : (oil sludge of tank bottom , oil sludge of oil pits) .Oil degrading bacterial species have been isolated on a selective mineral salt medium Bushnell and Hass Mineral Salt Medium (BHMS), supplemented with a carbon source. They are also identified depending on the Morphology and biochemical characteristics; four genus of bacteria (out of 89 bacterial isolates of oil sludge from the tank bottom)Bare identified. They include : 32 Pseudomonas spp. , 27 Rhodococcus spp. 21 Bacillus spp. , 9 Arthrobacter spp. , Also four genera of bacteria (out of 101bacterial isolates of oil sludge from the oil pits) are identified. They include : 41 Pseudomonas spp. 27 Rhodococcus spp. , 22 Bacillus spp. 11 Arthrobacter spp. , , While 8 species and 3 genus of bacteria (out of 259 bacterial isolates of the soil surrounding the oil pits) are identified. They include : 41 Aeromonas spp., 39 Corynebacterium spp., 39 Pseudomonas aeruginosa, 36 Bacillus spp., 33 Micrococcus leteuc, 23 Enterobacter intermedius, 22 Pseudomonas fluorescens, 12 Micrococcus varians 7 Staphylococcus epidermidis, 4 Staphylococcus saprophyticus,3Staphylococcus aureus .The biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes and two compounds of Pristane and Phytane are measured. Also, the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes and the two compounds of Pristane and Phytane are measured for each four genus which were isolated from the oil sludge pits, and it is also done for one isolated genus from the soil surrounding the oil pits. The measuring process was fulfilled as a comparative study with the control flasks. Thus, the oil sludge (tank bottom ) was used as a source of carbone in the first experiment While, the oil sludge (oil pits) was used as a source of carbone in the second experiment . The incubate time of the experiment is 27 days during which a lost of the percentage of hydrocarbon compounds each 9 days are recorded by using( Capillary Gas Chromatography ). The results showed the biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes of the oil sludge (tank bottom ) with regard toCbacteria 73.8 % Pseudomonas spp., 70.3 % Rhodococcus spp. 65.7 % Arthobacter spp., 55.6 % Aeromonas spp.,51.5 % Bacillus spp., Whereas, the biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes of the oil sludge (oil pits) with regard to bacteria 80 % Pseudomonas spp., 74.9 % Aeromonas spp., 70.4 % Rhodococcus spp., 64.8 % Arthobacter spp.,55 % Bacillus spp., While , The results showed of the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes of the oil sludge (tank bottom ) with regard to bacteria 73.7 % Pseudomonas spp.,70.3 % Rhodococcus spp. 65.6 % Arthobacter spp., 55.3 % Aeromonas spp. ,51.2 % Bacillus spp., Whereas, The results showed of the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes of the oil sludge (oil pits) with regard to bacteria79.2 % Pseudomonas spp., 73.8 % Aeromonas spp., 69.2 % Rhodococcus spp.,63.7 % Arthobacter spp., 53.7 % Bacillus spp. It was found that there is approximately match between the biodegradation of mole percentage by using the area and those by using the concentration for each component .

دراسة للطفيليات المعوية المعزولة من الخضروات المطرية من بعض اسواق مدينة بغداد

اسم المؤلف: وسن عداي مهدي المرسوي
الموضوع العام: علوم الحياة
السنة: 2012
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A study was carried out during the period from November 2010 to October 2011 to detect the intestinal parasites which contaminate the common fresh vegetables. A total of 303 samples were taken from three wholesale markets and three retail markets, in Baghdad province. Ten kinds common vegetables were selected. These vegetables were Celery (Apium graveolens); Parsely (Patroselinum crispum); Garden cress (Lepidium sativum); Leek (Allium porrum); Radish (Raphanus sativus); Lettuce (Lactuca sativa); Spinach (Ipomoea aquatica); Beet (Beta vulgaris); Mint(Mentha spicata) and Cabbage (Brassica oleracea).Sedimentation and floatation techniques were used for samples examination. Three different solutions (ZnSO4, saturated NaCl , saturated sucrose ) were used in floatation technique. The present study has been shown that the collected samples of vegetables were contaminated with sixteen species of intestinal parasites. It was found that the percentages of contamination with these parasites were as following : (Giardia lamblia) (29.4%), Entamoeba histolytica(24.5%), Entamoeba coli(16.9%), Balantidium coli (8%), coccidia(9.9%) and Blastocystis hominis(0.7%). For intestinal helminthes Ascaris lumbricoides (15.6%), Toxocara spp.(10.3%), Fasciola spp. (5.3%), Schistosoma haematobium (1.7%), Schistosoma masnsoni (1.4%), Taenia spp. (4.7%), Diphyllidum caninum (3.9%), Trichuris trichiura (3.3%), Enterobius vermicularis (6.9%) and Nematoda larvae (6.3%).The present study revealed that the percentage of contaminated vegetables by using sedimentation technique (72.3%) was significantly higher than that of flotation techniques (53.1%). Additionally it was found that, the different kinds of vegetables showed a significant difference in the percentages of contamination with the parasites in both techniques. Thus, the percentage of contaminated Garden cress was (93.5%), (72.4%) in both sedimentation and flotation technique respectively, while Cabbage showed (50%), (36.7%) respectively.A significant high percentage of contamination was recorded in Al - Nahrin wholesale market (89.2%) in comparison with low percentage (59.4%) in Al - Rasheed wholesale market. Additionally, it was found that the percentages of contaminated vegetables in wholesale (76.26%) were significantly higher from that of retail market (64.76%).The present study, also, showed that the percentage of detection of parasites by using ZnSo4 solution (31.2%) was more efficient than that showed by saturated sucrose (8.9%). Additionally; the soaking with normal saline (47.83%) showed a higher percentage of contamination than that by using distilled water (17.39%).The percentages of seasonal contamination were varied significantly, therefore, it was found that spring showed the highest percentage (29.7%) while summer the lowest (20.9%). The washed samples of Garden cress which obtained from the three wholesale markets, reduced the percentages of contamination with parasites but did not remove the parasites completely. Therefore, a significant difference has been noticed between washed (56.76%) and unwashed samples (76.66%).Concerning, the percentages of infections with intestinal parasites, it was found that the medical center (14 July) showed (29.4%) while Al - Ameria center (19.2%). Furthermore, the percentage of parasites infections was significantly higher in the summer (46%) than in winter (12.67%).The present study indicated that there were a positive coordination between the high percentages of contaminated vegetables and the high percentages of infection in the medical centers as in the case of G.lamblia and E.histolytica.Finally, it could be conducted that the contaminated vegetables played an important role in transmission of intestinal parasites to human, particularly, when these vegetables were eaten fresh without sterilization or good washing.

دراسة تشريحية ونسجية وكيمونسجية مقارنة للغدة الدرقية وجار الدرقية في نوعين من الفقريات العراقية (بيز ابن عرس، Herpestes javanecus والقنفذ طويل الاذن ، Hemiechinus auritus) باستعمال الواسم Anti - Calcitonin, code 140778) ) == Study of Thyroid and Parathyroid Gland in Two Iraqi Vertebrates ( Weasl , Herpestes javanicus and Long - Eared Hedgehog , Hemiechinus auritus) Using Marker (Anti - Calcitonin, code 140778)

اسم المؤلف: رنا علاء عبد الحميد هايس العامري
اسم المشرف: حسين عبد المنعم داود
الموضوع العام: علوم الحياة
السنة: 2016
الموضوع الدقيق: الحيوان - التشريح
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية التعرف على الوصف التشريحي والتركيب النسجي والكيمو نسجي - المناعي للغدتين الدرقية وجار الدرقية لنوعين من اللبائن العراقية ممثلة ب بيز ابن عرس ( ( Herpestes javanicus والقنفذ طويل الاذن ( Hemiechinus auritus ) وقد استعملت تقنيات مختلفة لانجاز الدراسة .• الغدة الدرقية Thyroid Glandاظهر الفحص التشريحي للغدة الدرقية في حيوان بيز ابن عرس (H. javanicus) البالغ (ذكور, اناث) ان الغدة تتكون من فصين (ايمن, ايسر) وتاخذ موقعا في منطقة العنق اسفل الحنجرة. ويتموضع فصا الغدة على جانبي الرغامى عند الحلقات (5 - 1) من حلقات الرغامى ولم يلاحظ وجود تركيب البرزخ (Isthmus) الذي يربط بين فصا الغدة , ويتباين لون الغدة بين البني والاحمر وفي الغالب تظهر بلون بني محمر(Reddish brown) يظهر فصي الغدة في شكل بيضوي او اهليجي, اظهرت نتائج الدراسة الحالية في حيوان القنفذ ( H. auritu ) تماثلا واضحا مع حيوان بيز ابن عرس فيما يخص الوصف التشريحي باستثناء كون فصا الغدة بيضويتي الشكل.تحاط الغدة في حيوان بيز ابن عرس بمحفظة من نسيج ضام (Connective tissue) تتكون من طبقتين, طبقة خارجية External layer)) تتالف من الياف مغراوية (Collagenous fibers) وقليل من الياف مرنة ((Elastic fibers ويتخللها ارومات ليفية Fibroblast)), وطبقة داخلية ((Internal layer تتالف من الياف مغراوية يتخللها الياف عضلية ملساء Smooth muscle fibers) ) ,كما تتضمن المحفظة اوعية دموية ((Blood vessels واعصاب (Nerves), تمتد من المحفظة الداخلية حويجزات رقيقة تقسم الغدة الى فصيصات غير مكتملة . اظهرت المحفظة في حيوان القنفذ تماثلا مع المحفظة في حيوان بيز ابن عرس.يتكون نسيج الغدة الدرقية في حيوان بيز ابن عرس بصورة رئيسية من الجريبات (Follicles) والخلايا الجنيب جريبية (Parafollicular cells) واوعية دموية واعصاب. تتخذ الجريبات اشكالا متعددة منها بيضوي (Oval), ومنها دائري ((Rounded, واحيانا تظهر بشكل غير منتظم (Irregular shape), وتكون الجريبات باحجام مختلفة صغيرة, متوسطة وكبيرة . اظهر القنفذ تماثلا مع حيوان بيز ابن عرس فيما يخص حجم وشكل الجريبات. تبطن الجريبات في بيز بن عرس اما بنسيج ظهاري مكعبي واطئ بسيط ( Low simple cuboidal epithelial tissue) او نسيج ظهاري مكعبي بسيط (Simple cuboidal epithelial tissue) , بينما في حيوان القنفذ يظهر نسيج ظهاري عمودي بسيط (Simple columnar epithelial tissue) فضلا عن النسيج الظهاري المكعبي الواطئ البسيط والظهاري المكعبي البسيط . تحتوي تجاويف الجريبات على مادة جيلاتينية هي الغروان Colloid)) , وتظهر الجريبات تباينا في كمية الغروان اذ ان بعض الجريبات تكون مملؤة بالغروان والبعض الاخر يحتوي على كمية قليلة والقليل منها يظهر خاليا تماما من الغروان في كلا الحيوانين موضوع الدراسة, تنتشر في نسيج الغدة خلايا تدعى بالخلايا الجنيب جريبية (Parafollicular cells) او تسمى خلايا الكالسيتونين (Calcitonin cells) او (C - cells), وتظهر هذه الخلايا باشكال متباينة يتراوح بين الدائرية (Rounded) الى مضلعة الشكل ( (Polygonal shape, وهي تظهر اما بشكل مفرد (Single) او بشكل تجمعات (Clusters) في نسيج الغدة, وتحتل هذه الخلايا موقعين اساسيين في نسيج الغدة , الاول داخل جريبي Intrafollicular position) ) وتكون بين الغشاء القاعدي والخلايا الظهارية الجريبية والثاني بين جريبي Interfollicular position)) وتكون بين جريبات الغدة الدرقية , وتكون قليلة العدد ولم تظهر اختلافا في حيوان بيز ابن عرس عن القنفذ .اظهر التفاعل الكيمونسجي المناعي في حيوان بيز ابن عرس والقنفذ استجابة واضحة للتفاعل المناعي مع الاجسام المضادة للكالسيتونين, وظهرت الخلايا متقبلة للون البني وهو دليل التفاعل المناعي .• الغدة جار الدرقية Parathyroid Gland توجد الغدة جار الدرقية بتماس مع الغدة الدرقية وتحاط بمحفظة من نسيج ضام وهي امتداد لمحفظة الغدة الدرقية والتي تتكون من الياف مغراوية والياف مرنة ((Elastic fibers والياف عضلية ملساء واوعية دموية واعصاب . توجد الغدة جار الدرقية في موقعين طرفي قمي وطرفي جانبي. تمتد من محفظة الغدة جار الدرقية حويجزات الى داخل الغدة لتفصلها الى فصيصات غير كاملة، وتنتشر داخل نسيج الغدة. اما في القنفذ ( H.auritus ) فانها تقع ضمن نسيج الغدة الدرقية وتظهر بشكل فصوص (4 - 2) فصوص دائرية او بيضوية الشكل محاطة بمحفظة رقيقة تفصلها عن جريبات الغدة الدرقية.تم تمييز ثلاثة انواع من الخلايا في نسيج الغدة جار الدرقية في حيوان بيز ابن عرس اذ تمثلت الخلايا الاكثر انتشارا بالخلايا الرئيسة ((Chief cells، والخلايا الحمضة (Oxyphil cells) وخلايا الماء الشفافة ( (Water - clear cellsوهي تماثل الخلايا في نسيج القنفذ وتترتب بشكل حبال خلوية ضمن نسيج الغدة.اظهرت الغدة جار الدرقية في بيز ابن عرس استجابة للتفاعل الكيمو نسجي المناعي, اذ ظهرت خلايا الغدة متقبلة للون البني دليل التفاعل المناعي اذ تظهر الاستجابة المناعية للخلايا على مستوى السايتوبلازم والغشاء البلازمي في الخلايا الرئيسة ( (Chief cells, واظهرت الاستجابة المناعية في الغدة للقنفذ اختلافا بالنسبة لشدة استجابة الخلايا حيث كانت اقل من حيوان بيز ابن عرس .اوضحت نتائج الدراسة الحالية الخاصة بتحليل الاحماض الامينية في مستخلص الغدة الدرقية وجار الدرقية في حيوان بيز ابن عرس (H.javanicus ) (ذكور، اناث), تشخيص (13) حامضا امينيا في الذكور و(11) حامضا امينيا في الاناث, اما في حيوان القنفذ H.auritus (الذكور، الاناث), فقد تم تشخيص (12) حامضا امينيا في كل من الذكور والاناث. | Anatomical examination of thyroid gland in adult (males &females) H. javanicus revealed that the gland consist of two lobes (right & left), situated below the layrnx in neck region. The two lobes located at the sides of trachea (1 - 5) rings of trachea. they appear without Isthemus. The colour of the gland is reddish brown. The shape of the lobes either to be oval or elliptical. On the other hand the thyroid gland in H. auritus showed a clear similarity with that of H. javanicus with the exception of the oval shape of the gland. Thyroid gland of H. javanicus surrounded by capsule of connective tissue consist of external layer of collagenous and elastic fibers, fibroblast and internal layer consist of collagenous fibers with smooth muscle fibers, the capsule also contain blood vessels and nerves. Thin trabeculae extend from the capsule divided the gland into incomplete lobules. The capsule of thyroid gland in H. auritus showed clear similarity with that of H. javanicus with the exception of the adipose tissue between the capsule and gland tissue in H. auritus.Histologically thyroid gland in H. javanicus consists of follicles, parafollicular cells, blood vessels and nerve. The follicles appear in different shapes (oval, rounded and irregular shapes, and have different size (small, medium and large). The thyroid gland tissue in H. auritus has the same appearance of that in H. javanicus. The follicules in H. javanicus lined by epithelial tissue either to be Lwo simple cuboidal or simple cuboidal epithelium. In H. auritus lind by Lwo simple cuboidal, simple cuboidal or even simple columnar epithelial tissue. The follicular cavities contain different quantities of colloid, and few of them lack of collid in both under investigated animals. Parafollicular cells are spreading in gland tissue; they also called calcitonin cella or c - cells. These cells have different shapes either to be rounded or polygonal shape, and found as single or clusters. They are either to be interfollicular or intrafollicular position. The immunohistochemistry reaction revealed clear positive reaction for calcitonin , and the appearance of brown colour represented evidence for the immune reaction .• Parathyroid glandResults of the present study revealed that parathyroid gland situated closely with thyroid gland in both animals under investigatin . The gland surrounded by a capsule of connective tissue represented as an extension from the capsule , which consist of collagenous fiber, elastic fibers, smooth muscles fibers and blood vessels and nerves. The parathyroid gland situated at two position (lateral terminal / apical terminal) the trabeculae wich exted from the gland capsule to the gland tissue separated the gland into incomplete. In H. auritus the parathyroid gland formed from (2 - 4) lobes with rounded or oval shapes and surrounded by thin capsule separated from the thyroid gland follicles.Three types of cells have been differentiated in the parathyroid tissue in H. javanicus represented by chief cells wich apper the most abundant in comparison with the other two types, the second type are the oxyphil cells and the third are water - clear cells, they are similar in both animals and appear as a cellular cords within the gland tissue. The immunohistochemical study in parathyroid gland of H. javanicus reveald positive reaction for calcitoninin, where ̛s the cells appear ̛s in brown color giving the evidence for the immune reaction in cytoplasm and plasma membrane of the chief cells In H. auritus there was clear immune reaction but less than H. javanicus . Result of the present study related with the amino acid analysis of the thyroid and parathyroid glands extraction in H. javanicus (mals &females) declear (13) amino acid in males and (11) in female while in H. auritus (male &females) there were (12) amino acid in both male and females.

اختبار كفاءة اوكسيد المغنيسيوم النانوي في مكافحة الفطر Rhizoctonia solani في نبات الباذنجان

اسم المؤلف: رشا سعد فوزي
اسم المشرف: امنة محمد علي | مهند مهدي عبد
الموضوع العام: علوم الحياة
السنة: 2018
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في مختبر السموم الفطرية / قسم وقاية النبات / كلية الزراعة / جامعة بغداد لسنة 2016 من اجل اختبار كفاءة جسيمات اوكسيد المغنيسيوم النانوي في مكافحة الفطر Rhizoctonia solani في نبات الباذنجان صنف (اعجوبة العراق ) .تم اختبار المقدرة الامراضية لعزلة الفطر Rhizoctonia solani في الوسط الزرعي PDA على بذور الباذنجان, اظهرت النتائج ان عزلة الفطر Rhizoctonia solani اعطت نسبة اصابة عالية على بذور الباذنجان مقارنة بمعاملة المقارنة (بدون فطر ) حيث توضح النتائج ان نسبة الانبات في معاملة المقارنة بلغت 97.33 %. اما في معاملة الفطر بلغت 12 % . كما وجد ان اضافة جسيمات اوكسيد المغنيسيوم النانوي ( MgO) بتراكيز 1 % و2 % و3 % ادت الى تثبيط الفطر مقارنة بمعاملة المقارنة (فطر ممرض ) وتراوحت نسب التثبيط (96.33 % , 100 % , 100 % ). في حين اظهرت نتائج تجربة الاصص ان معاملة البذور وخلط التربة بجسيمات اوكسيد المغنيسيوم النانوية له اثر ايجابي في رفع النسبة المئوية لانبات بذور الباذنجان وكبح نشاط الفطر Rhizoctonia.solani , وكذلك لها اثر ايجابي في معايير النمو المدروسة . اذ ادت المعاملة بجسيمات اوكسيد المغنيسيوم النانوية الى زيادة في الوزن الطري والجاف لنبات الباذنجان قياسا بمعاملة الفطر الممرض بمفرده الذي بلغ فيها الوزن الطري والجاف للمجموعين الجذري والخضري 0.18 , 0.06 , 1.60 , 0.52 غم.نبات 1ٙ على التتابع. في حين كانت اوزان المجموعين الجذري والخضري الطري والجاف لمعاملة البذور المعفرة باوكسيد المغنيسيوم النانوي 0.81 , 0.29 , 4.74 ,1.39 غم.نبات 1ٙ على التتابع, وفي معاملة التربة المعاملة باوكسيد المغنيسيوم النانوي 0.77 , 0.26 , 7.03 , 1.85 غم .نبات 1ٙعلى التوالي . وكانت معاملة المقارنة (بدون فطر ) 0.51 , 0.27 ,4.74 , 1.29 غم .نبات 1ٙعلى التتابع . واظهرت النتائج تاثيرجسيمات اوكسيد المغنيسيوم النانوية في نسب العناصرالغذائية (النتروجين , الفسفور , البوتاسيوم , الكلوروفيل ) في نبات الباذنجان فقد تفوقت المعاملات التي تحتوي على جسيمات اوكسيد المغنيسيوم النانوية في نسبة النتروجين فكانت نسبة النتروجين في معاملة البذورالمعفرة باوكسيد المغنيسيوم النانوي ومعاملة التربة المعاملة باوكسيد المغنيسيوم النانوي ومعاملة المقارنة(بدون فطر ) 2.04 , 2.03 , 1.99 ملغم . سمٙ2على التوالي اما في معاملة الفطر فكانت نسبة النتروجين فيها 1.77ملغم . سمٙ2 .اما نسبة الفسفور في معاملات البذور المعفرة باوكسيد المغنيسيوم النانوي والتربة المعاملة باوكسيد المغنيسيوم النانوي ومعاملة المقارنة (بدون فطر ) فكانت 0.51 , 0.48, 0.49ملغم . سمٙ2 على التتابع , اما في معاملة الفطر فكانت نسبة الفسفور فيه 0.47ملغم . سمٙ2 . وكانت نسبة البوتاسيوم في معاملات البذور المعفرة باوكسيد المغنيسيوم النانوي ومعاملة التربة المعاملة باوكسيد المغنيسيوم النانوي ومعاملة المقارنة(بدون فطر) 0.37 , 0.41 , 0.36 ملغم . سمٙ2 ,اما نسبة البوتاسيوم في معاملات الفطر فكانت 0.35 ملغم . سمٙ2 ,حيث تفوقت معاملة التربة المعاملة باوكسيد المغنيسيوم النانوي في نسبة البوتاسيوم فيها عن بقية المعاملات . وتفوقت معاملة التربة المعاملة بالنانو في نسبة صبغة الكلوروفيل فيها على بقية المعاملات مقارنة بمعاملة الفطر التي كانت نسبة الكلوروفيل A ( 1.01 , 0.54 مايكروغرام .سمٙ2) على التتابع , في حين كانت نسبة الكلوروفيل B في معاملة التربة المعاملة باوكسيد المغنيسيوم ( 1.17 , 0.71 مايكروغرام . سمٙ2) على التتابع .ان اضافة جسيمات MgO النانوية ادى الى زيادة نسبة كل العناصر الغذائية المهمة في المعاملات التي تحتوي على هذه الجسيمات . | This Study was conducted in mycotoxin laboratory / Defartment of plant protection / Agri - cuiture collage / Baghdad University in 2016 to test the efficiency of Magnesium Oxide - nano particles to combat Rhizoctonia solani fungi in eggplant type (Marvel of Iraq ). The satisfactory ability to isolating the Rhizoctonia solani fungi was tested in the agricultural sector PDA on eggplant seeds , The results showed that isolation of Rhizoctonia solani was given ahigh injury rate on eggplant seeds Compared to the comparison treatment (Without fungi ) . Where the results are shown the germination rate of comparison treatment was reached to 97.33% ,While in fungi treatment was reached to 12%. It was also found that addition of MgO nanoparticles in concentration (1%, 2% , 3%) led to inhibition the fungi compared to the comparison treatment (pathogenic fungi), the rate of inhibition varied (96.33%, 100% , 100%) .While the results of the experiments showed that the treatment of seeds and mixing the soil with MgO nanoparticles has positive effect to increase the percentage of eggplant seeds germination and curb the activity of Rhizoctonia solani fungi , Also it has positive impact on the studied growth parameters .The treatment of the MgO nanoparticles led to increase in soft and dry weight of eggplant compare with treatment of pathogenic fungi alone in which the soft and dry weight of radical and vegetative groups reached to (0.18, 0.06 , 0.52 gm) plant 1 on the relay . while the soft and dry weights of the radical and vegetative groups in the treatment of fermented seeds by MgO nanoparticles (0.81, 0.29 , 4.74 , 1.39 gm ) plant 1 on the relay . And in the soil treatment which was treated by MgO nanoparticles (0.77 , 0.26 , 7.03 , 1.85 mg) plant 1 on the relay .And the comparison treatment (without fungi ) was being ( 1.29 , 4.74 , 0.27 , 0.51 gm ) plant 1 on the relay . The results showed the effect of MgO nanoparticles in ratios of the nutrients (nitrogen , phosphour, potassium , chlorophyll )in eggplant , hence the treatment which containing MgO nanoparticles was excelled in nitrogen ratio subsequenliy the ratio nitrogen in the treatment of fermented seeds by MgO nano particles and soil treatment which treated by MgO nanoparticles and comparison treatment (without fungi )was being (1.99, 2.03 , 2.04 )mg.cm2 respectively. while in the treatment of fungi the ratio of nitrogen was (1.77mg .cm2 ). While the ratio of phosphorus in the treatment of fermented seeds by MgO nanoparticles and soil treatment which treated by MgO nanoparticles and comparison treatment (with out fungi ) was (0.49 , 0.48 , 0.51 mg. cm2) on the relay . while in the treatment of fungi , phosphorus ratio was (0.47 mg .cm2 ).And the ratio of potassium in treatment of farmanted seeds by MgO nanoparticles and soil treatment which treated by MgO nanoparticles and comperison treatment (without fungi ) was ( 0.37, 0.41 , 0.36 mg . cm2).While the ratio of potassium in fungi treatment was (0.35 mg.cm2) , where the soil treatment which treated by MgO nanoparticles was excelled in the potassium ratio than others treatments . The soil treatment which treated by nano was excelled in the chlorophyll dye ratio than others treatment compare with fungi treatment in which the chlorophyll A ratio was (1.01 , 0.54 mg.cm2) on the relay . whilst the chlorophyll B ratio in the soil treatment which treated by MgO was being (1.17 , 0.71 mg.cm2) on the relay . The addition of MgO nanoparticles led to increase ratio of the all nutrients which are important in the treatment containing this particles

اختبار كفاءة العسل المدعم بالمواد النانوية في مكافحة فطر Aspergillus flavus في بذور الذرة الصفراء المخزونة == Testing honey backed efficient nanomaterials in the against ( Aspergillus flavus) in Maize stored seeds

اسم المؤلف: ديمة ايوب يوسف مرزوق
اسم المشرف: امنة محمد علي | حليمة زغير حسين
الموضوع العام: علوم الحياة
السنة: 2018
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الى اختبار كفاءة جسيمات اوكسيد الزنك النانوية( ZnO(NP) ) والعسل والعسل المدعم بجسيمات اوكسيد الزنك النانوية ZnO(NP) ومستحضر الفايلكس في تثبيط الفطر Aspergillus flavus وتحطيم السم المنتج منه تحت الظروف المختبرية . اثبتت اختبارات التضاد ان اضافة كل من جسيمات اوكسيد الزنك النانوي ZnO(NP) بتراكيز 1%, 2%, 3% حققت تثبيطا لنمو الفطر A.flavusعلى الوسط الزرعي PDA بنسبة 98.55%, 100%, 100% على التوالي قياسا بمعاملة المقارنة (فطر ممرض بمفرده والتي بلغت (0.0 , واظهرت نتائج استعمال العسل بتراكيز 10%,20%,%30 الى تثبيط الفطر بنسبة %75.3 ,%78 ,78.6 % على التوالي, في حين بينت النتائج فاعلية العسل المدعم ب NP))ZnO في تثبيط الفطرعند استعماله بتراكيز %10 , %20 , %30 اذ بلغت نسبة التثبيط %85.6, %87 , 87.3 % على التوالي. كما اظهرت النتائج كفاءة مستحضر الفايلكس في تثبيط الفطر بنسبة 74.4%, 100%, 100% عندما كان التركيز المستعمل من الفايلكس 0.1%, 0.2%, %0.3على التوالي. تم اختبار كفاءة جسيمات ZnO(NP)والعسل والعسل المدعم بجسيمات ZnO(NP) ومستحضر الفايلكس تحت ظروف الخزن بينت النتائج ان جميع المعاملات ادت الى خفض معنوي في نسبة الاختزال وتركيز سم الافلا B1))AFB1 في حبوب الذرة الصفراء المخزونة وبفارق معنوي عن معاملة السيطرة اذ بلغت 0.0و 1.13مايكروغرام/كغم باستعمال جهاز High performance liquid chromatography (( HPLC , باختيار افضل تركيز لكل من المواد المستخدمة لتثبيط نمو الفطر , اذ اظهرت نتائج استخدام جسيمات ZnO(NP) ومستحضر الفايلكس اعطى اعلى نسبة اختزال لسم الافلاB1 بلغ% 93.80 وبتركيز 0.07 و91.15% بتركيز 0.10مايكروغرام/كغم على التوالي كما اظهرت نتائج استعمال العسل نسبة اختزال سم الافلا B1 %79.64 وبتركيز 0.23مايكروغرام/كغم , وتلاه استعمال العسل المدعم بجسيمات ZnO(NP) اذ بلغت نسبة اختزال سم الافلا B1 %82.30 وبتركيز 0.20مايكروغرام/كغم . اظهرت النتائج كفاءة جسيمات ZnO(NP)والعسل والعسل المدعم بجسيمات ZnO(NP) ومستحضر الفايلكس في تحطيم الفطر A.flavusومنع انتاج سم الافلا B1 في حبوب الذرة الصفراء قياسا الى معاملة السيطرة التي كانت 1.12مايكروغرام/كغم باستعمال جهاز HPLC , اذ وجد عند استعمال جسيمات ZnO(NP) نسبة اختزال سم الافلا B1 %96.42وبتركيز 0.04 مايكروغرام /كغم , في حين كانت نسبة اختزال السم %72.32وبتركيز 0.31مايكروغرام /كغم عند استعمال العسل , وبينت نتائج استخدام العسل المدعم بجسيمات ZnO(NP) نسبة اختزال سم الافلا %83.92 B1وبتركيز 0.18 مايكروغرام /كغم , اظهرت نتائج استخدام مستحضر الفايلكس نسبة اختزال سم الافلا %94.64 B1 وبتركيز 0.06 مايكروغرام/كغم . | This study was aimed at efficacy assessment of zinc oxide nanoparticles ZnO(NP), honey, honey fortified with ZnO(NP) and phylex chemical against Aspergillus flavus and its toxin at culture media . Antagonism tests revealed all ZnO(NP) treatments at 1%, 2% and 3% inhibited A. flavus growth on PDA by 98.55, 100 and 100%, respectively compared to untreated control (fungus only control) which was 0.0%. Honey treatments at 10, 20 and 30% inhibited the fungus growth by 75.3 , 78 and 78.6 %, respectively. ZnO(NP) - fortifie honey treatments inhibited fungal growth by 85.6, 87 and 87.3 % at 10, 20 and 30%, respectively. Whereas, phylex treatments could inhibit fundal growth by 74.4%, 100% and 100% at concentrations 0.1, 0.2, 0.3, respectively . ZnO(NP), honey, ZnO(NP) fortified honey and phylex efficacy at storage condition were tested. All treatments inhibited A. flavus growth and reduced the aflatoxin (AFB1) production in stored corn. HPLC confirmed ZnO(NP) treatment reduced AFB1 up to 93.80 when aflatoxin concentration was 0.07 μg/kg compared to 1.13 μg/kg for control treatment. Whereas, honey, ZnO(NP) fortified honey and phylex treatments reduced B1 aflatoxin up to 79.64, 82.30. and 91.15%, respectively. B1 concentration values tested for each treatment were 0.23, 0.20 and 0.10 μg/kg compared to1.13 μg/kg for control treatment. The efficacy of ZnO(NP), honey, ZnO(NP) fortified honey and phylex to damage the fungus A. flavus and prevent AFB1 production in corn grains was tested by HPLC. ZnO(NP) reduced B1 aflatoxin up to %96.42 with 0.04 μg/kg concentration compared to 1.12 μg/kg for control treatment. Honey, ZnO(NP) fortified honey and phylex treatments could reduce B1 aflatoxin up to 72.32, 83.92 and 94.64%, respectively. B1 concentration values tested in corn grains were 0.31, 0.18 and 0.06 μg/kg compared to1.12 μg/kg for control treatment indicating presence of significant differences

تاثير الجبرلين والكاينتين والسماد المركب NPK في نمو وحاصل الينسون (.Pimpinella anisum L) ومحتواه من بعض المركبات الفعالة == The effect of Gibberellin , Kinetin and compound NPK fertilizer on growth and yield of anise (Pimpinella anisum L.) and some its active compounds

اسم المؤلف: غفران علاء محمد رضا الخزرجي
اسم المشرف: بهاء الدين مكي فيروز الربيعي
الموضوع العام: علوم الحياة
السنة: 2017
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت دراسـتان حقليتان في الحديقة النباتية التابعة لقسم العلـوم - كلية التربية الاساسية - الجامعة المستنصرية خلال موسم النمو الشتوي 2015 - 2016 تهدم الدراسة الاولى الى معرفة تاثيرتراكيز من منظم النمو الجبرلين ( (GA3 ومستويات من السماد المركب NPK والتداخل بينهما وتتضمن التجربة الثانية معرفة تاثير من منظم النمو الكاينتين ومستويات من السماد المركب NPK والتداخل بينهما التجربة الثانية في بعض صفات النمو والحاصل والمواد الفعالة للزيت الطيار في نبات الينسون (.Pimpinella anisum L) , زرعت بذور الينسون بتاريخ 1/11/2015 اذ استخدمت ثلاثة مستويات من السماد المركب NPK (0 و200و 400) كغم.ھ - 1 اضيفت الى التربة فضلا عن ثلاثة تراكيز من حامض الجبرلين هي 0) و50 و100) ملغم.لتر1 - رشت على النباتات في التجربة الاولى اما التجربة الثانية فقد تضمنت ثلاثة مستويات من السماد المركب NPK (0 و200و 400) كغم.ھ - 1 اضافة الى ثلاثة تراكيز من الكاينتين (0 و50 و100) ملغم.لتر1 - , صممت التجربة وفق تصميم القطاعات الكاملة المعشاة RCBD وبثلاثة مكررات وبذلك تضمنت كل دراسة تجربة عاملية (3×3) بواقع 27 وحدة تجريبية مساحة كل وحدة تجريبية 1.5×1.5 م2 اتبعت كافة عمليات خدمة التربة والمحصول الخاصة بمحصول الينسون من ري وازالة الادغال وتسميد. اظهرت نتائج التجربة الاولى ان اضافة المستوى 400 كغم.ھ - 1 من السماد المركب NPK ادت الى زيادة ارتفاع النبات (13.24%) ووزن الف بذرة (20.21%) وعنصر البوتاسيوم (20%) والمركبات الفعالة Dianethole وTrase - anethole وLimonene وγ - himachalene , كما ادى رش نباتات الينسون بالتركيز 100 ملغم.لتر1 - من حامض الجبرلين الى زيادة ارتفاع النبات (14.88%) ووزن الف بذرة (7.77%) وعنصر البوتاسيوم (7.81%) , وزيادة تركيز المواد الفعالة Trase - anethole وLimonene وShikmic acid, اعطى التداخل بين السماد المركب NPK وحامض الجبرلين زيادة معنوية في بعض الصفات المدروسة اذ ادى التداخل بين المستوى 400كغم.ھ - 1 من السمادNPK وتركيز 100ملغم.لتر1 - من حامض الجبرلين الى زيادة في ارتفاع النبات (29.38%) ووزن الف بذرة (29.41%) وعنصر البوتاسيوم (27.94%) والمركبات Methyle chavicol وDianethole وTrase - anethole وγ - himachalene وShikmic acid. اما في التجربة الثانية فقد ادت اضافة المستوى 400 كغم.ھ - 1 من السماد المركب NPK الى حصول زيادة في ارتفاع النبات (9.65%) والبوتاسيوم (39.20%) ووزن الف بذرة (10.96%) فضلا عن المركبات Anise - aldehyde وMethyle chavicol وDianethole وTrase - anethole وγ - himachalene . كما عمل التركيز 100 ملغم.لتر1 - من الكاينتين على زيادة عدد الاوراق (26.31 %) وعدد التفرعات (43.54%) والوزن الجاف (28.05%) وعدد النورات (26.26%) وعدد ازهار للنورة الرئيسة (26.14%) وعدد البذور في الزهرة الواحدة (26.72 %) وحاصل النبات الواحد (88.66%) وحاصل النبات الكلي (88.59%) وعنصر النتروجين (62.83%) والفسفور (62.96%) والكالسيوم (62.18%) والمغنيسيوم (60.86%) والبروتين (62.71%) والكاربوهيدرات (38.69%) والكلوروفيل (44.17%) والنسبة المئوية للزيت (37.84%) والوزن النوعي (1.07%) وكثافة الزيت(0.51%) ومعامل الانكسار (0.32%) اضافة الى المركبات Anise - aldehyde وMethyle chavicol وDianethole وTrase - anethole وγ - himachalene وShikmic acid , اما التداخل بين التركيز 100 ملغم.لتر1 - من الكاينتين والمستوى 400 كغم.ھ - 1 من السماد المركب NPK اعطى زيادة في المركبات Anise - aldehyde وDianethole وTrase - anethole وγ - himachalene . يستنتج من هذه الدراسة تفوق المستوى 200 كغم.ھ - 1 من السماد المركب NPK في معظم الصفات المدروسة ولكلا التجربيتن اما الجبرلين فقد تفوق التركيز 50 ملغم.لتر1 - تفوقا في معظم التراكيز المدروسة , واعطى التركيز 100ملغم.لتر1 - من الكاينتين تفوقا في معظم الصفات المدروسة .

دراسـة لبعــض المؤشــرات المناعيــة والدمــوية لمرضى عراقيــين يعانــون من فــرط الحساسيه النــوع الاول == Study of Some Immunological and Hematological Changes in Iraqi Patients Suffering from Hypersensitivity Type - 1

اسم المؤلف: سرى مسلم داود الاعــــــرجي
اسم المشرف: طالب عبد الله حسين
الموضوع العام: علوم الحياة
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: قد تم تصميم هذه الدراسة لتسليط الضوء على دور بعض الحركيات الخلويه ( IL - 13, IL - 18) والكلوبيولين المناعي الكلي ((T - IgE والكلوبيولين النوعي(S IgE) ل 20 مسبب للحساسيه داخلي وخارجي واعداد خلايا الحمضات. وكان مجموع مرضى الحساسية 168، [99 مريضا بالربوالتحسسي (43 ذكرا)، (56 انثى)]، [69 مريض بالتهاب الجيوب الانفيه التحسسي (29 ذكرا)، (40 انثى)]، وجمعت اربعين عينه لاشخاص اصحاء [ (15 ذكور)، (25 اناثا )]. الفئة العمرية (5 - 60) سنة تم جمعها من المركز التخصصي للحساسية في بغداد / الرصافة وخلال الفترة من تشرين الثاني 2016 الى شباط 2017. تمت دراسه بعض التغيرات الدمويه ومنها قياس نسبه ال (Hb) والعدد الكلي لخلايا ال (WBC) واعداد خلايا الحمضه لجميع المرضى والاصحاء عن طريق استخدام محلل الالي (Beckman Coulter) والعده الخاصه بالجهاز . تم قياس الاختبار المناعي (IL - 13, IL - 18, T - IgE) لجميع المرضى والاصحاء باستخدام جهاز (ELISA ) والعدد الخاصه بالاختبارات. تم قياس الكلوبيولين النوعي (Spesific IgE) الخاص بالمحسسات المستنشقه (عث الفراش، الحيوانات الاليفه ,الصرصر، الاعفان، حبوب اللقاح الاعشاب، حبوب اللقاح الاشجار ,حبوب اللقاح الحشائش) لجميع المرضى والاصحاء باستخدام تقنيه (Western Immunobloting Electrophoresis test) والعده الخاصه بالجهاز والفحص (Euro line Inhalation Profile Kits).توصلت هذه الدراسه الى النتائج التاليه : 1 - عدم وجود فروق معنويه بين المجاميع المدروسه طبقا للجنس . 2 - عدم وجود فروق معنويه بين مرضى الربوالتحسسي الذين لهم تاريخ عائلي ايجابي وسلبي. بينما كانت هناك فروق معنويه بين مرضى التهاب الجيوب الانفيه التحسسي الذين لديهم تاريخ عائلي سلبي وايجابي .3 - احصائيا، كانت الفئة العمرية و(21 - 40) الاكثر شيوعا في مرضى الربو التحسسي والتهاب الجيوب الانفيه التحسسي حيث بلغت نسبتها المئويه على التوالي[63، 63.6٪)، (47،68.1٪)].4 - زيادة معنوية في النسبه المئويه لاعداد الحمضات (٪) ومستوى الكلوبيولين المناعي الكلي ( T - IgE) ظمن مجموعتي الحساسية مقارنتا بمجموعه السيطره.5 - اظهرت النتائج وجود فروق معنويه بين اعداد المرضى الذين لديهم الكلوبيولين المناعي الخاص بالمحسسات ((Spesific IgE واعداد المرضى ذوي النتيجه السالبه لدى مرضى الربو التحسسي والتهاب الجيوب الانفيه التحسسي . 6 - سجل محسس الصراصر اعلى نسبة محسس لدى مرضى الربوالتحسسي وتلتها مسببات الحساسية للحيوانات الاليفة وحساسية حبوب اللقاح. بينما في مجموعة التهاب الجيوب الانفيه التحسسي اعلى نسبة محسسات كانت للحيوانات الاليفة وتلتها حبوب لقاح الحشائش .7 - زيادة معنويه كبيرة في مستوى الحركيات الخلويه (IL - 13, IL - 18) عند مرضى الربو التحسسي والتهاب الجيوب الانفيه التحسسي .8 - وجود علاقة ارتباط معنوية كبيرة بين النسبه المئويه لاعداد خلايا الحمضات والكلوبيولين المناعي الكلي( T - IgE) في مرضى الربو التحسسي والتهاب الجيوب الانفيه التحسسي r = 0.424)، (P = 0.00 | This study is designed to shed light on the role of IL - 13, IL - 18, T - IgE, Spesific IgE for 20 Indoor and outdoor inhalant allergens in some of type - 1 allergic disease and Eosinophil cells count . The Total allergic patients have been 168, 99 asthmatic patients (43 male ), (56 female ), 69 Rhinitic patients (29 male ), (40 female) and Apparently Healthy Control AHC 40 (15 male ) , (25 female ). Their age group (5 - 60) years . The groups have been collected from the Specialized Center Of Allergy in Baghdad / AL - Resafa and during the period from October 2016toFebruary2017. Some Hematological changes have been studied, including the measurement the Hb% , total count WBC, and eosinophil cells % for all subject by using automated analyzer (Beckman Coulter Analyzer). Beside some Immunological changes were studies including (IL - 13, IL - 18 and total IgE) which have been measured for all subjects by using Enzyme Linked Immunosorbent Assay (ELISA) . The measurement of serum specific IgE for some inhalant allergens (HDM, Pits, Cockroach, Mites, Grasses pollen, Tree pollen and Weed pollen ) allergens for all subject by using Western Immunobloting test. The Study has reached the fallowing results.1 - There are no significant differences between the studied groups according to gender.2 - There are significant differences in family history between rhinitis group and control. 3 - Statistically, the age group (21 - 40) are most frequent within allergic asthma and rhinitis are (63, 63.6%), (47,68.1%) respectively. 4 - Highly significant increase in Eosinophil count (%) and total IgE within allergic groups when compared with control. 5 - Highly significant differences between positive specific IgE inhalant allergens (positive) and negative patients in asthma and rhinitis groups. 6 - The cockroach allergen records the highest percentage in asthmatic patients fallowed by pets allergens and grasses pollen allergens. While in rhinitic group the highest percentage is for pets allergens fallowed by grasses pollen , cockroach allergen. 7 - Highly significant increase in IL - 13 and IL - 18 level in asthmatic and rhinitic patients. 8 - Highly significant correlation between eosinophil cells count (%) and total IgE In asthmatic and rhinitic patients (r = 0.424 ), (P=0.00).

دراسة تصنيفية مظهرية لعائلة حشرات الخنافس الحارقة Coleoptera : Meloidae في محافظة بابل == Taxonomical and Morphological study on the Blister Beetles (Coleoptera : Meloidae) in Babilon province

اسم المؤلف: اميرة ابراهيم عبد الزهرة
اسم المشرف: سعدون ابراهيم اسماعيل
الموضوع العام: علوم الحياة
السنة: 2017
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study deals with morphology and taxonomy of blister Beetles (Coleoptera : Meloidae) in Babylon province.In our investigations ; there were fifteen species representing in nine genera under two subfamilies have been registered, these species are : 1 - Epicauta hirticornis (Haag - Rutenberg) Croscherichia goryi (Marseul)10 - Lydomorphus sp. MaranCerocoma graeca - 1112 - Cerocoma scovitzi Faldermann13 - Lydus mesopotamicus sp. nov.(Linnaeus) 14 - Lydus algiricus Fabricius Lytta nitidula - 2 - Nemognatha chrysomelina (Fabricius)sp. 3 - Nemognatha4 - Zonitis immaculate (Olivier)5 - Zonitis fernancastroi, Pardo Alcaide6 - Mylabris sp.klug 7 - Mylabris syriaca8 - Mylabris tenebrosa Laporte de CastelnauThe species of Lydus mesopotamicus sp. nov. has been described as a new species for science in Iraq, and has been given scientific geographical name.The genus Zonitis Fabricius and its two species; and the genus Lydomorphus Fairemaire have been recorded for the first time in Iraq.Three other species has been diagnosed and recorded for the first time in Iraq, they are : Epicauta hirticornis (Haag - Rutenberg)Fabricius Lytta nitidulaMaranCerocoma graecaThe Epicauta hirticorins (Haag - Rutenberg, 1880) was chosen as a simple type species of this family ; adults are described in details and all species body parts were drawn and pictured.Taxonomic keys for subfamilies, genera and species were desigend according to different of the important characters, especially pronotum, antennae, claws, spines of hind tibiae and pathches of elytra.

العلاقة بين المشعرات المهبلية والعوامل المرضية التناسلية الاخرى بين النساء في مدينتي كركوك وتكريت == Relationship Betwee Trichomonas Vaginalis And Other Genital Infectious Agents Among Women In Kirkuk & Tikrit Cities

اسم المؤلف: برهان احمد محمد علي بيباني
اسم المشرف: يحيى جرجيس سلمان | ابراهيم شعبان داود
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم خلال الفترة من الخامس عشر من شهر تشرين الاول 2006 ولغاية الثلاثين من شهر حزيران 2007 اجراء دراسة عن وبائية طفيلي المشعرات المهبلية وبعض مسببات الالتهابات المهبلية الافرازية المرافقة وغير المرافقة لها بين النساء المراجعات لقسم الاستشارية النسائية والتول | An epidemiological study was carried out from 15th, October 2006 to 30th, June 2007 for prevalence of Trichomonas vaginalis parasite and some causative agents of secretary vaginal inflammations associated or non - associated with the parasite among women attending the department of Obstetrics and Gynecology in two General hospitals in Kirkuk city and Tikrit teaching hospital in Tikrit city. A total of 300 samples (200 in Kirkuk, 100 in Tikrit) were colle - cted from (15 - 47) years old women. Two swabs were taken from the vaginal (high vaginal) and the lining of uterus cervix (endocervical) for each woman by Gynecologic physician. A gross examination of the swabs was done to determine the color of vaginal secretions and measurement of pH. A wet preparation was done from the (high vaginal swab) and examined microscopically for diagnosis of Trichomonas vaginalis and Candida spp. In addition a Gram stain smear was prepared from a portion of the same swab and examined by oil - immersion for diagnosis of Neisseria gonorrhoeae and Trichomonas vaginalis. A chemical test was done for the detection of Gardnerella vaginalis bacteria & Candida spores. The swabs prepared from endocervical were treated by ACON - Chlamydia Kit specific for diagnosis of Chlamydia trachomatis in vaginal swabs samples. The study concluded the following results : 1 - The infection with various sexually transmitted diseases agents were 68% , 78% in Kirkuk & Tikrit cities respectively (P>0.05).2 - The maximum infection rate for T. vaginalis was 19.5% in Kirkuk city & 16% in Tikrit city among co - infection with other agents and single infections.3 - The percentages of single infections with T. vaginalis alone for various age groups were 16.5%, 8% in Kirkuk and Tikrit cities respectively (P>0.05).4 - The rate of infections with various vaginal micro - organisms were in the following sequence : T. vaginalis (19.5%), G. vaginalis (18.5%), C. trachomatis (14%), Candida spp. (11.5%) & N. gonorrhoeae (4.5%) in Kirkuk city, whereasin Tikrit city the sequence of infection rates were as follows : G. vaginalis (43%), T. vaginalis (16%), C. trachomatis (12%), Candida spp. (6%) & N. gonorrhoeae (1%).(P>0.05).5 - The highest rates of infections with T. vaginalis only as a single infecting agents were 21.27%, 10% among a group age range (36 - 45) years in both Kirkuk & Tikrit cities respectively (P<0.05). 6 - The maximum rate of infection with the T. vaginalis were recorded among women with yellowish vaginal secretions without itching (37%), (31.25%), whereas in those with symptoms of copious secretions onlythe corresponding figures (30.17%), (18.51%) were recorded in bothcities respectively (P<0.05). 7 - The maximum infection rate with C. trachomatis in bloody swabs were (40%), (50%) in Kirkuk & Tikrit cities respectively correlated withclinical signs & symptoms. However, maximum infection rate with G. vaginalis was recorded in women with whitish vaginal discharge (60.86%) or yellowish (53.12%) then greenish (52.94%) in Tikrit city in comparison to Kirkuk city. The maximum infection with G. vaginalis were among women with bloody secretion (40%) followed by whitish secretion (30.35%). (P<0.05). 8 - The maximum infection rate with Candida spp. were recorded in women discharge milky secretions (33.33%), (25%) in Kirkuk & Tikrit cities respectively. Infection with N. gonorrhoeae showed different color vaginal discharge (P>0.05).9 - The maximum rate of infections with T. vaginalis (27.43%), (19.69%) were recorded in women whose pH of vaginal secretion range was between (5 - 6). (P> 0.05). The high vaginal swabs have been revealed to be more efficient in diagnosis of vaginal infection with T. vaginalis than endocervical swabs. The maximum rate of infections recorded by these swabs were (22.22%), (19.73%) respectively. The infection rate of pregnant women were (48%), (22.22%) from positive cases in both cities.10 - The maximum rate of infections with T. vaginalis were recorded in women using various contraceptive, women using intrauterine contraceptive device were the highly infected (33.33%), (31.57%) in both cities. P<0.05

دراسة تاثير البروبايويك في تكوين الغشاء الحيوي وانتاج البروتيز لبكتريا Pseudomonas aeruginosa المعزولة من اخماج الحروق والجروح == Study The Effect of Probiotic On Biofilm Formation And Production Protease Enzyme By Pseudomonas Aeruginosa Isolated From Contaminated Burns And Wound

اسم المؤلف: علا عبد الكريم كاظم النعيمي
اسم المشرف: منيرة جلوب اسماعيل العبادي | ضيماء محمود ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: he study includes the collection of 70 burns and wound swabs from patients of different hospitals, in Baghdad area, for the period from November 2013 to February 2014. 2) 31 isolates characterized as Pseudomonas aeruginosa 9 (45%) from wound and 22 (78.57%) from burn swabs, depending on the characteristics of the colonies phenotypic and microscopic when grown in differential selective media, as well as biochemical tests todiagnose isolates, Then the identification of these species were confirmed by using the system (GN I Card) using Vitek 2 device complementary step.3) The study includes sensitivity test towards 16 antibiotics for each species by using the system (AST Card) using Vitek 2 device, and the isolates ware Multi drug resistance, As results all isolates were resistant by 100 % for each of Piperacillin, Ticarcillin, Ticarcillinclavoulanic acid, Cefazolin, Ceftriaxone and Tigecycline. All strains were less resistance to Ceftazidime with percentage 50%. 4) All the isolates of Pseudomonas aeruginosa with percentage %100, have ability to produce protease enzyme.5) This study was use tow methods of biofilm formations seen in P. aeruginosa, Micro - titer plate method (MTP) and Tube method (TM), Results revealed that MTP was found to be more sensitiveand specific method for biofilm detection than TM. 6) Well diffusion method revealed the inhibitory effect of bacteria Lactobacillus acidophilus, Lactobacillus rhamnosus GG and its supernatants in the growth of bacteria Staphylococcus aureus and P. aeruginosa, and was the supernatant of L. rhamnosus GG isthe largest inhibitory effect on the growth of S. aureus and P.aeruginosa with inhibition diameter 32 and 34 mm respectively, while the inhibition of growth by supernatant of L. acidophilus diameter 25 and 27 mm respectively. 7) Estimated the amount of protein in each of the biosurfactant and bacteriocine producers of L. rhamnosus GG bacteria concentration of 74, 54 Mg/mL respectively, while their concentration in bacteria L. acidophilus 44, 40 Mg / mL, respectively.8) Found that the bacteriocine product of bacteria L. rhamnosus GG and L. acidophilus GG have inhibitory effect on ability of P. aeruginosa to produce the enzyme protease, and the absence of this effect in the Biosurfactants the product of these two types.9) The biosurfactant product from L. rhamnosus GG showed inhibitory effect greater than the effect of the biosurfactant product from L. acidophilus to inhibition (production of biofilm and adhesion to epithelial cells).10) studied the suspension's effect of L. acidophilus and L. rhamnosus GG in contrived injuries wounds in mice contaminated with P. aeruginosa, Positive results have been shown to be affected by the contaminated bacteria injuries wounds.

تقييم دور الجين في دم نساء عراقيات مصابات بسرطان الثدي == Evaluation The Rule of Mammoglobin A Gene In Blood of Iraqi Women With Breast Cancer

اسم المؤلف: نانسي فيصل رشيد
اسم المشرف: امنة نعمة الثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Mammaglobin A is a member of the superfamily of secretoglobins. Its expression is highly specific for mammary tissue and has been shown to be overexpressed in breast tumor tissue, indicating that mammaglobin A might confer a growth advantage to mammaglobin A - expressing tumor cells. The present study was undertaken to develop the mammaglobin A as a serum biomarker for the early detection of breast cancer, and breast cancer patients follow up after treatments. This study had taken ten months, it involved 84 patients with age ranged 15 - 74 years who had diagnosed with either stage I to III breast cancer or benign lesions of breast at certain Iraqi hospitals and breast cancer centers, and a total number of 10 apparently healthy individuals, with comparable age range of patients as control group.Ten ml of venous blood was collected from studied groups in order to be used for measuring estrogen and progesterone levels, Reverse transcription polymerase chain reaction (RT - PCR) test for detection of their serum m - RNA mammaglobin A existence, and serum mammaglobin A levels using Enzyme - Linked ImmunoSorbent Assay (ELISA) technique. Demographic study revealed that most cases enrolled in this study were women within their reproductive age 25 - 44 years old. Distribution of patients according to age group, marital status, and lactation status showed no statistically significant association (P>0.05) with any of these variables, in all comparison.The hormonal study states that elevated levels of estrogen were observed in 52 (62%) patients, also decrease in progesterone levels were observed in 50 (59%) patients out of the 84 cases studied. This indicate a highly significant SUMMARYassociation (p < 0.01) between breast cancer risk, elevated estrogen levels, and decrease in progesterone levels. Mammaglobin A protein was detectable by ELISA in 100% of breast cancer patients (65 case), and not in any benign tumor patients (19 case) nor healthycontrol women (10 females) which indicate the highly specificity of ELISA test (P<0.01) for measuring mammaglobin A levels in patients serum. With high concentration of protein in sera of women with late stage of breastcancer compared with a low protein concentration in sera of women with early stage of breast cancer. By applying a statistical analysis on the result it was found that serum mammaglobin A ELISA test is highly significant (P<0.01) for, differentiation between patients with metastatic breast cancer and patients with early diagnosed breast cancer. In this work, SCGB2A2 gene which coded for mammaglobin A was detected in 64 out of 65 breast cancer cases, but not in the benign or healthy individuals indicating its high specificity as a marker gene (SCGB2A2) for cells derived from mammary glands. A statistical analysis was made for comparison between the three groups, E - Malignant Tumors \ Early Stage, L - Malignant Tumors \ Late Stage, and B - benign, (E, L, and B) which show that the results of molecular study of mammaglobin A detected by RT - PCR is statistically high significance (P<0.001) when used for the differentiation between E group and B group, also between L group and B group. The results showed that mammaglobin A can become an important tool for detecting primary and metastatic breast cancer, and predicting disease outcome.

دراسـة بيئيــة للافقريات القاعيـة في هور الدلمج - وسط العراق == An Ecological Study of The Benthic Invertebrates In Al Dalmage Marsh - Middle of Iraq

اسم المؤلف: ميسون حسن مشجل السراي
اسم المشرف: هيفاء جواد جوير
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية التنوع الاحيائي لمجتمع لافقريات القاع في هور الدلمج / وسط العراق فضلا عن بعض الخصائص الفيزياوية والكيمياوية لمياه الهور، اختيرت ست محطات لجمع العينات وذلك للمدة من بداية كانون الثاني وحتى كانون الاول 2013. استخدم تحليل التوافق CCA | Benthic invertebrate diversity on the Al - Dalmage marsh in the middle of Iraq, in addition to the some physical and chemical properties was the goal of the present study, for a period from January to the end of December 2013. Six stations along the eastern side of the marsh were chosen for monthly samples collection.The results showed water temperature ranged from(9 - 34.1)?C. The pH values were neutral but tends to near alkaline and ranged from (7.2 - 9).The Electrical conductivity values varied from (22735 - 2330) ?s/cm, according to the values of salinity (1.5 - 14.6) ‰ the marsh was classified as mesohaline (TDS) which showed high values ranged from (1820 - 17762) mg/L.This study showed that the marsh were well oxygenated due to the dissolved oxygen values which ranged from 7 - 12 mg/L, the study recorded values to BOD5 were ranging from (1.5 - 3)mg/L, and the marsh classified as very hard according to the total hardness values from (6980 - 825) CaCo3 mg/L. The high perecentage of organic matter in the sediment was 2.7 %. The results showed the station were characterized by sediment texture varied from silt clay sand sediment in the stations 1, 4, 5 , silt clay in St(2) and sand silt in St(3), sand clay silt in St(6).A total of 21286 individual benthic invertebrates was sorted, which represents which 78 taxonomic units, including 9 taxa. The Crustacea was the dominate group representing 29% of the total number of benthic invertebrates. It was noted that there were obvious differences in the total number of sorted individuals in which varied in each location and each month. The highest total number recorded in was st (2) was 4360 including 73 taxonomic units, while the highest total number recorded in April was 2624.The results of The relative abundance index showed that the species insects : Polypedilum sp Were more abundant in St(2 - 6), and dominant in St(6), while the Seinura sp were dominant in St(6), while the other taxonomy units varied from less abundant to rare abundant. Also the results of constancy index showed The which were considered "constant " However the other taxonomy units varied from "accessory " and" accidental" taxonomy units in study stations.The Shanon - Weiner index of totall benthic invertebrates varied from (1.2 - 3.8) bit/Ind, The uniformity index of benthic invertebrates varied from(0.7 - 1), these high values indicate that there is no ecological stress on benthic invertebrates in the study area.The Results of Jaccard Presence - community of similarity showed the highest degree of similarity to be 96% which recorded between St(1) and St(2).The values of the index of spcies richness varied from 1.3 - 15 with the greatest values recorded in April while lower values were in October.Six species of Turbellarian Platyhelminthes that were recorded, from which Stenostomum leucops, Stenostomum bryophilum, Stenostomum unicolor, Gyratrix hermaphrodites, Macrostomum sp, Dalyelliidae, which were considered as new records for Iraq, with Gyratrix hermaphrodites as the most abundant species and representing 25% of the total number of Turbellarian. It was noted that there were obvious differences in the total number of sorted individuals which varied in each location and each month, the highest total number recorded in St 3 was 400, while the highest total number recorded in November was (198).The present study result showed that three species of Nematoda were recorded incuding Seinura sp, Alaimus sp, Dorylaimus sp, Seinura sp as the most abundent species representing 84% of the total number of Nematoda. Spatially the highest total number of 590 individuals was recorded in St (4) , while temporally, the highest total number of 366 was recorded in October.One unidentified species of Gastrotricha was recorded for the first time in Iraq, 102 Ind were sorted these were considered "constant" recording to constancy index 63.8% which were sorted from all stations.A total of 2319 individuals of Rotifera was sorted, these were identified as 15 species that included Philodina sp, Rotaria citrinus, Dipleuchlanis sp, Euchlanis sp, Notholca sp, Ttriploeuchlainis sp, Colurella sp, Lepadella sp, Lecane sp, Proales sp, Cephalodela sp, Synchaeta sp, Hexarthra mira, and finally Brachionus sp which was recorded the highest number of individuals 540 individuals.A total of 1888 individual Annelida were sorted representing 12 species including nine species belonging to the family Naididae (three belong to the sub family Naidinae and six belonging to Tubificoid worms), two species belonged to Aleosomatidae including Aeolosoma hemprichi, Aeolosoma variegatum, and finaly one specie belong to Hirudinea which was Glossiphonia heteroclite, with three 3 ind in all study.The family Aleosomatidae recorded the highest percentage of 58% of the total number of Annelida.The sub family Naidinae including Paranais litoralis, Dero (Aulophorus) furcata, Stylaria lactustris.The highest frequency of 23.6% was recorded by P. litoralis.The Tubificoid worms represent six species which were Limnodrlius hoffmeisteri, Limnodrlius profundicola, L. claparedeianus, L udekemianus, Tubifex tubifex, Branchiura sowerbyi. This study also isolated 6238 individual Crustacea, these were identified as 15 species that included five species belonged to Cladocera, four order belong to copepod, which were recorded the highest percentage 30% of the total number of Crustacea, in addition to Macrobrachium nipponense from Decapoda, Sphaeroma annadalei anndalei from Isopoda, Ostracoda, Amphipoda, Conchostracha. It was noted that there was obvious differences in the total number of sorted individuals which varied in each location and each month, The highest total number recorded in st (2) was 1480, while the highest total number of 730 was recorded in April. The study finds 4297 individual insects groups, were sorted represent seven order includes larvae of Ephemeroptera, Trichoptera, Odonata, (Zygoptera, Anisoptera) Diptera (Culicidae, Chironomus sp, Polypedilum sp) Lepidoptera larvae Hemiptera (Corixidae, Nepa cinerea), and adult of Coleoptera (Dysitidae, Hydrophilidae Elmidae, Curculionidae, Coleoptera larvae), the Diptera larvae were recorded the highest percentage 55 % of the total number of insects groups, and Nepa cinerea was considered as new records for Iraq.This study showed two class from Mollusca, 1956 Ind were sorted, including Gastropoda and Pelecyopoda.The Gastropoda which were recorded the highest percentage 94% of the total number of Mollusca, these were identified to physa acuta, Lymnaea sp, Melonoides tuberculata, Melnopsis nodosa, Theodoxus sp, physa acuta which was the greatest percentage 41% of the total number of gastropoda. pelecyopoda which were including Pseudodontopsis euphraticus, Tigridis Unio, Corbicula fluminea, Corbicula fluminali

دراسة تصنيفية حياتية مقارنة لانواع من الجنس Euphorbia L. (Euphorbiaceae) في العراق == A Comparative Biosystematic Study of Some Species of The Genus Euphorbia L. (Euphorbiaceae) In Iraq

اسم المؤلف: ازهار طاهر صليبي
اسم المشرف: عذية ناهي سلمان المشهداني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تصنيفية مقارنة لثلاث وعشرين مرتبة تصنيفية تعود جميعها للجنس Euphorbia L. من العائلةEuphorbiaceae النامية في العراق، تضمنت الجوانب المظهرية العامة والدقيقة ودراسة حبوب اللقاح والمركبات الكيميائية والبيئة والتوزيع الجغرافي فضلا عن ا | The current research deal with twenty - three taxa belong to the genus Euphorbia L., Family Euphorbiaceae grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also studied and the chemical compounds, Ecological and geographical distribution and taxonomic treatment have done.Also botanical key was putted to separate the species. General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens studied, and correct the mistake naming, specimens were identified and reidentified some miss identified specimens in the Iraqi herbaria. A comparative morphological study was undertaken to all plants parts of taxa appeared from habit and duration of taxa to the quantitative and qualitative attributes the members of the vegetative and floral parts included the roots, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying with natural images for different morphological characteristics, as well as the field images. This study showed that all species was herbs, shrubs and succulent and semisucculent annual and perinnal, the major taxonomic importance was for flowering parts includeing the specific Inflorescence, installation of a cupped and its regularity on the branches of the plant, glands forms, numbers, presence or lack of appendages, bractes and bracteoles shapes, dimensions and color fruits shape, indumentum and seeds forms, dimension and her surface sculpturing; and the presence or lack of caruncle, as well as the qualities of roots, stems and leaves, and the morphological characteristics of all plant parts mentioned above have a taxonomic importance in identification and separation of the studied taxa. The study of the micromorphological characters of hairs, leaves epidermal cells and the stomatal complex which included anomocytic, paracytic, anisocytic and tetracytic, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa.The pollen grains have a taxonomic importance because their different in shape, dimensions of polar and equatorial view, the wall thickness and their ornamentation. They are mostly of Tricolporate except two species were Hexacolporate, also the differences in their shape in the polar and equatorial view.They were spheroidal, ovoid and oblate - spheroidal in the polar view and oblate, spheroidal, subprolate and oblate - spheroidal in the equatorial view.The ornomentation they were reticulate, microreticulate and foveoate.The chemical study also importance in separation of the genus taxa four compounds Flavonoids diagnosed in all the studing species but different in concentrations, by using HPLC, they are Myriscetin, Quercetin, Kaempferol and Coumarin esculetin and this confirms that there are a common evolutionary link between the species in terms of their chemical properties, which suggests belonging species to the genus Euphorbia.Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical districts. the species of Euphorbia were distributed in all Iraqi districts especially in LCA, MSU, MRO, and DWD and some species were farmed in gardens and nurseries either to the beauty of their bractes colors or because their acceleration to the environmental changes such as succulent species, As well as wild species were spread in mountainous areas, oak, nuts forests, in orchards, cultivated fields, flat plains and desert areas in loam soils, clay, gravel, and sand. Through this study some new taxa where founded in Iraq and they are E. cyparissias Kunth ; E. maculata L. ; E. serpens L. ; E. tirucalli L., and this study suggests to recorded them for the first time in Iraq.

دراسة بيئية وسكانية للقوقع الارضي Candidula gigaxii (L.Pfeiffer, 1850) (Pulmonata : Hygromiidae) في مواقع مختارة من محافظة بغداد == Ecological And Population Study of The Land Snail Candidula Gigaxii (L.Pfeiffer, 1850) (Pulmonata : Hygromiidae) In Selected Locations From Baghdad Province

اسم المؤلف: بشرى محمد كاظم ديوان البيضاني
اسم المشرف: عماد الدين عبد الهادي المختار
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Few studies on terrestrial snails in general, and garden snails in particular, existin Iraq. The group being important apparently for its wide - spread distribution in various habitats. One species of this group which has been previously recorded in Baghdad, is the garden snail Candidula gigaxii, class : Pulmonata, family : Hygromiidae.Available samples of the land snail C.gigaxii were collected from four locations within Baghdad province; a Taji, the Army Canal area, the Zafaraniyah, and Al - Jadiriya district, the study period of June 2013 to May of 2014.Environmental measurements were recordedat each site. Results showed that air temperature range was 10 - 34? C, soil temperature range was 5 - 26? C, pH range of the soil was between 4 - 8.9 and the content of the soil organic matter was between 0.3 - 2.0%.Snail samples were divided into seven size - classes according to the dimension of the shell and these were <2, 2 - 4, 4 - 6, 6 - 8, 8 - 10, 10 - 12, >12 mmPercentages of each size class were calculated. Having measured shell dimensions, shell growth index (Ia) was also calculated. The range of growth index value for this snail was 1.9 - 3.86 where the highest value was recorded at the Taji site in January 2014 which was probably attributed to low temperature range and high soil moisture and organic matter content.Results also showed that C. gigaxii favored soils with moderately high pH values and rich in organic content. Soil moisture appeared to be a limiting factor for growth; and a loamy soil texture for existence of the studied species. Average highest population density of living snails and empty shells were 44 and 32 individuals / m2 respectively. The overall population density was also calculated for the purpose of obtaining realistic population density of the species.

دراسة تصنيفية لحشرات عائلة الخنافس الرواغة Staphylinidae : Coleoptera في بعض محافظات العراق

اسم المؤلف: كاظم عادل هادي
اسم المشرف: سعدون ابراهيم اسماعيل
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:

دراسة نوعية مياه خزان الحبانية موقعيا والاستشعار عن بعد == Study of The Water Quality In Alhabbaniyah Reservoir In Situ And Remote Sensing

اسم المؤلف: احمد عطا الله حسن الفهداوي
اسم المشرف: عادل مشعان ربيع
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة المائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

العلاقة بين مؤشر الموت الخلـوي المبرمج (ربيطة فاس الذائبة) وقلة النطاف عند الرجال == Correlation Between Apoptotic Marker (Sfas Ligand) And Oligozoospermia In Men

اسم المؤلف: احمد حسين علي الزاملي
اسم المشرف: صباح ناصر العلوجي | انعم رشيد الصالحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The Fibroblast associated (Fas) system in the testes has been identified as a key regulator of apoptosis, a process that greatly influences the germ cell population of the testes. and the soluble Fas Ligand (sFasL) may be a competitive inhibitor to induce apopto¬sis of the Fas baring cells. Therefore, this study measures the level of sFasL in the semen of oligozoospermic men evaluating the association between seminal plasma sFasL and spermatogenesis.A total 58 oligozoospermic men and 29 normozoospermic volunteers were included in this study. They were attending the Infertility Clinic of the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, AL - Nahrain University\ Baghdad. Their age mean was 31.77± 5.48 years and a median of 30 years. Semen samples were evaluated according to World Health Organization 2010 standard parameters. The Seminal plasma was separated from the cellular elements after centrifugation and the supernatant was transferred to plane tubes and frozen at - 20 ?C till using for measurement of sFasL. These patients were classified into patients with severe oligozoospermia (sperm concentration ?5 million / ml) which include 29 patients, and patients with mild and moderate oligozoospermia (sperm concentration ranging between 5 - 15 million / ml) which includes 29 patients as well. The control group comprised of 29 men with normospermic parameters according to WHO 2010 standard (Normal volunteers). The sFasL was measured using ELISA enzyme immunoassay for quantitative determination of sFasL Kit. Data were analyzed statistically using Analysis of variance (ANOVA), Least Significant Difference (LSD) and Correlation Coefficient (r).The sFasL level was found significantly higher (P< 0.01) in seminal plasma of oligozoospermic men, with weak correlation of the level of sFasL with the degree of severity of oligozoospermia. Based on the results of this study, it was concluded that sFasL is a novel marker found in the seminal plasma of oligozoospermic men. It's level was higher in cases of oligozoospermia regardless of its severity.

تكامل جرعة منخفضة من مبيد الترفلان مع مخلفات زهرة الشمس في مكافحة الادغال وتحسين نمو محصول الماش لتقليل الاعتماد على المبيدات والحد من مخاطرها == Integration of Reduced Dose of Trifluralin Herbicide With Sunflower Residues For Weed Control In Mungbean Field

اسم المؤلف: اﺭﻭﻯ ﻋﺒﺪ ﺍﻟﻜﺮﻳﻢ ﺗﻮﻓﻴﻖ
اسم المشرف: اﺑﺮﺍﻫﻴﻢ ﺷﻌﺒﺎﻥ ﺍﻟﺴﻌﺪﺍﻭﻱ
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments, several greenhouse and laboratory experiments were conducted during the course of study to evaluate the allelopathic potential of two sunflower (Helianthus annuus L.) cultivars on companion weeds and weeds grown in mungbean (Vigna radiata L. R. Wilczek) field alone and in combination with reduced rate herbicide, and to determine the chemical and genetical bases of allelopathic traits in the test cultivars. The aim of the first experiment was to test whether the variation in weed growth between the sunflower cultivars (Shumoose and Sin Altheeb), which was observed in the field, was due to differences in their allelopathic potential. Results showed the ability of both cultivars to reduce weed population and biomass with the superiority of Shumoose cultivar in suppression of weed density at 60 and 120 days after sowing compared to Sin Altheeb. Weed biomass was significantly reduced up to 90 and 71% by Shumoose and Sin Altheeb cultivars, respectively. Stair case experiment indicated that root exudates of Shumoose cultivar showed more suppression to weeds than Sin Altheeb giving additional evidence for the superiority of Shumoose cultivar in its allelopathic weed suppression. Chemical analysis by High performance liquid chromatography indicated the presence of 9 compounds in root exudates of Sin Altheeb and 8 compounds in Shumoose, and all are Phenolic in nature. However, total of the isolated phenolics was doubled in Shumoose than in Sin Altheeb. Most of the isolated chemicals are reported to have inhibiting ability for germination and growth of plants, including weeds. Second field experiment was undertaken to explore the response of weeds and mungbean crop to soil incorporated with sunflower residues in combination with lower rate of a pre - plant herbicide (trifluralin). Sunflower residues of the two cultivars Shumoose and Sin Altheeb at 3.2 and 6.4 t ha - 1 were used either alone or in combination with trifluralin at 1.2 L ha - 1 (50% of label rate). Weedy check (control), trifluralin at full label rate (2.4 L ha - 1), and weed free treatments were also included for comparison. Plots treated with 50% of label rate of herbicide and amended with sunflower residues of cultivar Sin Altheeb recorded least weed density and dry biomass and this suppression was much greater than the residue treatments alone and more than cultivar Shumoose. Application of herbicide at 50% rate in plots amended with Sin Altheeb residue resulted yield 64 % more than with the label rate of herbicide treatment. Chromatographic analysis of sunflower amended field soil revealed the presence of several potent allelopathic compounds in the residues of both cultivars with greater quantity (355.5 µg/ml) in Sin Altheeb than in Shumoose (250.9 µg/ml). Dynamics of release, decomposition and degradation of allelochemicals into the soil showed that different compounds exhibited differential behavior for these processes. Overall quantity of allelochemicals started to increase after 2 weeks of decomposition and peaked at 4 week of decomposition (180.1 ppm) then declined sharply in their quantities thereafter. Periods indicating higher quantities of total phenolics as shown by chromatographic analysis was coincided with the periods in which higher suppressive activity against weeds grown under field conditions. Bioassay of sunflower residues decomposed in soil at different times on Echinochloa colonum L, one of the weeds dominated the mungbean field, revealed that residues of both cultivars suppressed growth of E. colonum weed. The phytotoxicity started after 2 weeks and persisted for 8 weeks. However, Sin Altheeb residues showed greater inhibition than Shumoose cultivar at the last three decomposition periods. Shumoose residues recorded appreciable reduction to the growth of test weed compared to Sin Altheeb. The effect of residues of both cultivars on chlorophyll content and ions uptake in Amaranthus retroflexus L. weed revealed that residues of both species reduced chlorophyll content of leaves and the reduction increased with the increased residue rate. Sin Altheeb cultivar residues were more inhibitory than Shumoose at the lower residue rate. In the same weed, ions uptake was significantly averted by the residues of both cultivars. P and K was significantly increased over control, while Ca, Mg an Fe were found to be decreased over control due to application of sunflower residues in soil at 8 g per kg soil. N was the only test element that did not significantly influence by the residue treatments. No significant differences were found in the test ion uptake between the test sunflower cultivars. Results of using RAPD technique on the sunflower genomic DNA revealed that all the 19 primers used in this study scored different amplification monomorphic and polymorphic bands in the tested genotypes with 12 primers generated polymorphic bands. These bands present in one cultivar and absent in another. They could be responsible for allelopathic trait or secondary compounds associated with this phenomenon. Further work is recommended to analyze the sequence of this band to find out whether it is related to allelopathic trait or not.

التنميط الجيني لفايروس التهاب الكبد نوع اي لمرضى الاصابة الحادة في محافظة المثنى - العراق == Genotyping of Hepatitis E Virus Among Patients With Acute Infection In Al - Muthanna Province / Iraq

اسم المؤلف: ازهار صبري مسلم الذهبي
اسم المشرف: رغد حربي مهدي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Hepatitis E Virus (HEV) infection is found worldwide. It is responsible for large outbreaks in East and South Asia. This study was carried out to identify the distribution of acute HEV infection in AlMuthanna province and then determine the genotypes responsible for the disease spreading. This is the first investigation about the occurrence of hepatitis E genotypes in Al - Muthanna province patients. The study included 270 jaundiced patients of both sexes From AlMuthanna province which included out patients in public health laboratory in Samawah city, for the period from October 2013 to June 2014. All these patients were tested for anti - HEV IgM antibodies by using enzyme linked immunosorbent assay (ELISA). In Al - Muthanna province, 72 patients (26.66 %) out of 270 patients showed positive results for anti - HEV IgM antibodies, and all those patients were tested for confirmatory test at central public health laboratories (CPHL) in Baghdad province. Those patients consist of 45 females and 27 males with age ranged between (4 - 74) years old, were all negative for routinely screened markers of Hepatitis A, Hepatitis B and Hepatitis C, Ten normal healthy individuals, used as normal control in this study. This study showed that the HEV IgM is more common among younger age group (15 - 24), with a percentage of) 41.67%(, and it was higher in females (63%) than in males (37%). Highly significant differences (p ? 0.01) appeared among age groups. The levels of liver function enzymes demonstrated significant differences (p ? 0.05) in HEV patients as compared with healthy controls. The levels of Total Serum Bilirubin (TSB) were (23.41 ± 12.38) mg/dl, Serum Aspartate transaminase (SAST) were (887.17 ± 9.87) U/l, Serum Alanine transaminase (SALT) were (859.91 ± 13.28) U/l, and the levels of Serum Alkaline phosphatase (SALP) were (206.56 ± 5.04) U/l higher than healthy controls (0.884 ± 0.05) mg/dl, (37.27 ± 4.58) U/l, (34.14 ± 7.63) U/l, (48.09 ± 15.42) U/l respectively. RNA was extracted from sera of positive anti - HEV IgM antibodies by using a QIAamp® Viral RNA Mini Kit. Nanodrop and Quantus™ Fluorometer instruments were used to determine the RNA yield and purity according to the standard kit protocol. High levels of RNA concentration were measured by Nanodrop and Quantus™ Fluorometer. Forty - six samples have high RNA concentration out of 72 samples were detected for genotypes I, III & IV by using Applied Biosystems Real time PCR 7500 machine. In this study, HEV type I and III were detected in 23 samples out of 46 samples by using Real Time PCR systems. Ten samples were positive for this test. Thirteen samples (56.52%) were negative for this test. The distribution percentage of genotypes I and III was (43.48%). The results appeared significant difference (P ? 0.05). Also by using the same technique (Real time PCR systems) the HEV type IV was detected in 23 samples out of 46 samples. Which included, 13 samples that recorded negative results by I & III genotypes kit and only 2 samples from it recorded positive results and 11 samples recorded negative result for HEV genotype IV. From other 10 samples, only 3 samples recorded positive result and 7 samples recorded negative result for HEV genotype IV. In a total, 5 samples were positive for HEV genotype IV and 18 samples (78.26%) were negative. So that the distribution percentage of genotype IV was (21.74%). In this study, the samples that showed negative results in both kits have not been tested for other genotypes. This study indicated that the acute HEV infection is highly endemic in Al - Muthanna province in Iraq. Genotypes I & III were the most distribution than genotype IV in patients from Al - Muthanna province in Iraq. These results suggest that the genotypes I & III are the main causative agents of sporadic HEV infection in Al - Muthanna province

تاثير بعض العوامل البيئية في مجتمع الهائمات القشرية لنهر دجلة وجزيرة بغداد السياحية - العراق == The Effect of Some Environmental Factors In Crustaceans Plankton Community of The Tigris River And The Island of Baghdad Tourist - Iraq

اسم المؤلف: اعتدال فليح حسن العامري
اسم المشرف: صباح فرج عبد الاحد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study was conducted to investigate the effect of the water body type on the density of crustaceans zooplankton of the cortical Cladocera and two orders of copepod (Cyclopoida, Calanoida), addition to the physical and chemical effect of lake of Baghdad Tourist Island and Tigris river within the water of the lake near the site and electric and know the effect these characterties on density of crustaceans Zooplankton and for doing this study. We choose five places, tow on Tigris river and three places on the Island in AI - Fahhama in north of Baghdad The samples of water were collected every 15 days all these stations from beginning of December 2013 to May 2014. This study had corrected the temperature of air and water and the pH, electrical conductivity. and Dissolved oxygen, BOD5, NO?¯, PO?¯ and the density of Crastaceans Zooplankton. This study showed that the temperature of the air was nearly in the river and the lake and that because of their being in the same geographs area.The temperature was between (16°c - 34°c)in Tigris while it was (11.9°c - 33°c)in the lake Hydrogen Ions, it was (7.3 - 7.9) in the river and (8.18.8)in the lake because that were a lot of water plants and phytoplankton. This study shows that water of the river and the lake in the areas of study have a good of air and that was shown by recording the heigh values of oxygen. The values of dissolved oxygen (DO) was between (8.11 - 11.5) mg\L in river between (7.9111.37)mg\L in lake and showed from the study that the values were few. It was less than 5mg\L it was (0.33 - 2.6) mg\Lin the river and (0.64 - 3.55) mg\L in the lake. The values electrical conductivity of water in all the placed the study in the permissible range the water, it isn't over than 1500?s\cm. The electrical conductively records were between (851 - 1184) ?s\cm in the river, and it was (755 1270) ?s\cm in the lake. From the other hand nutrients, the phosphor records between (0.025 - 0.0563)mg\Lin the river and in the lake between(0.00310.0429)mg\L The Nitrate records its values highs in the month of winter because of the rain and it was increased in the river, it was (0.345 - 0.9135)mg\L compare with the lake, it was (0.028 - 0.626)mg\L in the places of the study that were appearance of two clear increases in the density of Zooplankton one was in winter and the other was spring.The density of zooplankton in the lake much more than it was in the river. The density of the zooplankton belong to Cladocera between (0.019 - 0.307) ind.\L in the lake and the density of the Calnoida was between (0.003 - 0.065)ind. \L in the river and (0.0078 - 3.37) ind. \L in the lake. The results shows differences if there are changes in places and month on most of physical and chemical biological characteristic the measured during the period of this study. The kind of water body effects on most characteristics and soon the density of crustaceans zooplankton.the results of the study mention the binding morale relation between the density of zooplankton the values of electrical conductivity and value of BOD(Biological Oxygen Demand) while there isn't any morale relation between the density of zooplankton and the value of Hydrogen Ion and Nitrate and dissolved oxygen that because the value of these changes was near to be equal in all the places that eayses the reduce of its effective on the average of density of these organism. The study shows that Baghdad lake have the environmental ingredients makes the lake natural protected to forming and breeding a fish in the lakes in addition that it is a place for Tourism.

تاثير عصارة نبات البروكلي في متلازمة تعدد الاكياس المبيضية المستحدثة في الجرذ الابيض == Effect of Broccoli Plant Juice On Induced Polycystic Ovarian Syndrome In Albino Rats

اسم المؤلف: رقية احمد صالح
اسم المشرف: نهلة جاسم محمد الشاهري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The goal of present study was to investigate the benefits of broccoli as anti androgenic plant in protection and treatment for reproductive hormonal and metabolic disturbance, which combined with polycystic ovarian syndrome (PCOS). Three experiments for 3 PCOS models were done. First Experiment : The prepubertal female rats at 21 days old, were injected with Testosterone (T) subcutaneously for 39 days (first model). The females of this experiment were divided into 6 groups (8 rats each) as follows : Group 1 was injected with sesame oil. Group 2 was injected with T hormone. Group 3 was injected with T hormone with orally gavaged of broccoli juice. Group 4 was injected with sesame oil, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 5 was injected with T hormone, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 6 was injected with T hormone, and at the end of the last injection was orally gavaged with broccoli juice for 30 days. Second Experiment : The prepubertal female rats at 21 days old, were injected with Letrozole (L) subcutaneously for 39 days (second model).The females of this experiment were divided into 6 groups with the same style of the first experiment. Third Experiment : it was achieved by the injection of pregnant rats prenatally (days 16 to 19 of gestation) with 1 mg of T hormone subcutaneously (third model), and then the female offspring were divided into groups (8 rats each) as follow : The first offspring group : The female rats offspring at 21 days old (their mothers were injected with sesame oil from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with distilled water : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with broccoli juice : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with broccoli juice until 60 days of age. The third offspring group : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation and orally gavaged with broccoli juice) were orally gavaged with distilled water until 60 days of age. Females of all groups were sacrificed after 24 hours from the last treatment to undertake weight, histological, hormonal and metabolic study for each group. the results of present study showed the symptoms that reported PCOS of the three female experiments such as ovarian cyst, hyperandrogenemia, hormonal and metabolic disturbance, etc. Also a well improvement has been noticed in all syndrome symptoms on females gavaged with broccoli juice for all three rats PCOS experiments, these results indicate that broccoli juice has got a protective and curative role against this syndrome.

التحري الجزيئي عن النمط الثالث لداء السكري البادئ عند النضج (MODY 3) في مرضى السكري العراقيين == Molecular Investigation of Maturity Onset Diabetes of The Young Type 3 (MODY 3) In Iraqi Diabetic Patients

اسم المؤلف: اسراء عدنان ابراهيم البغدادي
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: شملت الدراسة 63 مريضا مصابا بداء السكري غير المناعي المنشا في اعمار مبكرة من ذوي التاريخ العائلي بالاصابة بمرض السكري ممن لايعانون البدانة الذين تم اختيارهم من مرضى السكري من مراجعي المركز التخصصي لامراض الغدد الصم والسكري في الرصافة/ بغداد للفترة من ايار | This study included 63 non obese patients having early onset of non immunogenic diabetes with strong family history of diabetes selected from diabetic patients attended the specialized center of endocrinology and diabetes at Alrusafa/ Baghdad during period from the begging of May 2011 till the end of February 2013. The demographic study revealed that there is no association between the disease incidence with neither the gender nor the blood group. But there is a significant difference in the distribution of the patients according to age of the first diagnosis and gender, as the study showed that majority of male patients (53.846%) were first diagnosed with hyperglycemia below the age of 20 while the majority of females (72.972%) were first diagnosed with hyperglycemia after the age of 20.The distribution of patients according to the type of treatment also reveled association between the gender and both age of diagnosis and type of treatment of the hyperglycemia as the study showed that there is a higher percent of male patients (80.768%) using insulin at the beginning of their diagnosis with hyperglycemia or transferred later to insulin than those of females (67.565%).The present study reported for the first time in Iraq the presence of monogenic diabetes (maturity onset diabetes of the young type 3) as major cause of diabetes within non obese diabetic patients' early onset of non immunogenic diabetes with strong family history of diabetes. The sequence analysis of the hepatocyte nuclear factor 1? gene showed that Iraqis have mutational hot spot at exons 3 and 4 of this gene responsible for maturity onset diabetes of the young type 3, and that mutations in the promoter region or exons 5, 6, 8 and 9 are a rare cause of diabetes.

دراسات وراثية خلوية ومظهرية لثلاثة انواع من شبوطيات المياه العذبة العراقية (Barbus luteus, Cyprinion macrostomus, Chondrostoma regius) == Three Species of Iraqi Freshwater Cyprinidaes (Barbus Luteus, Cyprinion Macrostomus, Chondrostoma Regius)

اسم المؤلف: اسماء سامي ابراهيم
اسم المشرف: ندى عبد المجيد الانصاري | طه ياسين الدوري
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الحيوان - الوراثة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية التعرف على الطراز الكروموسومي ونظام تحديد الجنس والتمييز بين الجنسين مظهريا في ثلاثة انواع من اسماك المياه العذبة العراقية ممثلة بسمكة الحمري Barbus luteus والبنيني كبير الفم macrostomus Cyprinionوالبلعوط الملوكي Chondrostoma regius | These present studies were carried out to determine the karyotypes & the system of sex determination between the two sexes as well as the sexual morphological characteristics of three species of Iraqi fresh water fish Barbus luteus, Cyprinion macrostomus, & Chondrostoma regius that were fished from Tigris river at Baghdad during 2005. The cytogenetic studies revealed that the chromosome number of Barbus luteus is 2n = 148 & the karyotype of males is (80m + 52sm + 16st) while the females has (80m + 51sm + 17st). The chromosome number of Cyprinion macrostomus is 2n = 50 the males has (6m + 24sm + 12st + 8t) while the females has (6m + 23sm + 13st + 8t). The chromosome number of Chondrostoma regius is 2n = 48 and the karyotype is (14m + 30sm + 4st). Sex chromosomes were identified in Barbus luteus & in Cypinion macrostomus for the first time , the sex is determined by ZZ / ZW system, the female was considered as heterogametic (ZW) while the male was considered as homogametic (ZZ) , & the chromosome Z was represented as submetacentric chromosome & chromosome W as small subtelocentric chromosome. In spite of the difference in their chromosome number , the ratio between red blood cell diameter & it's nucleus was approximated in these species. The differences among these species in both of the diameter of cell and the nucleus didn't match with the differences at the polyploidy levels or with the difference in chromosome number within the same level. The liver & intestine were recommended as new sources for chromosomal studies , because of the good ratio of dividing cells (71.4 % & 58.2 %) respectively from the whole dividing cells in kidney that was considered as a control. The crude aqueous extract of fresh convolvulus arvensis leaves showed good effect in arresting the cell division of fish at metaphase , the concentration (0.4, 0.6, 0.8, ) mg / gm arrested kidney cells of carp fish at the metaphase in ratios 71.1 % , 88.1 % , 94 % respectively from the colchicines treated kidney cells (control). Positive significance correlation was found (r = 0.9518) between the percentages of arrested cells at metaphase & the concentrations of crude extract. Comparison between the females and males of the three species revealed that in Barbus luteus the distance from head edge to the anal orifice & from head edge to the dorsal fin were larger in males than females. The females have dark colored fins with reddish ventral aspect of head & body in comparison with the males during the whole months of study. So , they were considered as permanently sexual dimorphism characteristics. But , during the spawning seasons (May & June ) a more brighting color of male with reddish coloration of both sides of body were noticed in comparison with female with a golden orange corneal coloration in both sexes. Thus, they were considered as temporary sexual dimorphism characteristics. In Cyprinion macrostomus the distance from the head edge to the anal orifice was larger in females than males. The colors of anal & caudal fins were darker in females than in males during the whole monthes of study.So , they were considered as perminantly sexual dimorphism characteristics. While, during the spawning seasons ( May , June , July & August) , the gill operculum was of orange color only in male that extend to the end of pectoral fin. So , this was considered as a temporary sexual dimorphism characteristic. In Chondrostoma regius the distance from the head edge to the dorsal fin & from the dorsal to caudal fin as well as the pectoral fin base length were larger in females than in males during the whole months of study it was considered that they are permanently sexual dimorphism characteristics. Differential characteristics were not noticed during the spawning season (February & March). The permanently sexual dimorphism & the general characteristics were not affected by the differences of standard length , weight , age & season , because they were observed in fishes of different lengths , weights & ages & during the months of study 2005. But , the seasons affect the development of temporary characteristics in Barbus luteus & Cyprinion macrostomus , because these characteristics were observed during the spawning season only. Aging of Barbus luteus was accompanied by growth increase as indicated by the mean of standard length during May , July, August, September & December & by the mean of weight during May , August, September, October & December. A positive correlation was found between standard length & weight during the months of study , with the exception of January. While aging of Cyprinion macrostomus & Chondrostoma regius was not accompanied by growth increase as indicated by the means of standard length & weight , but a positive correlation between standard length & weight was found during the studied months. Same growth type were observed in the three species of fish , it was allometric in two sexes.

دراسة تصنيفية مظهرية لبعض انواع عائلة قفازات الضفادع من رتبة متشابهة الاجنحة Homoptera : Cercopidae من بعض محافظات العراق == Taxonomic Morphological Study of Some Species of Froghoppers, (Homoptera : Cercopidae) From Some Iraqi Governorates

اسم المؤلف: حلا تايه عمران
اسم المشرف: حسن سعيد جاسم الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمن البحث دراسة تصنيفية مظهرية لخمسة عشر نوعا تابعة لاربعة اجناس لعويلتيCercopinae، Aphrophorinae لعائلةCercopidaeمن رتبة متشابهة الاجنحة Homoptera جمعت من مناطق مختلفة في العراق تم الاعتماد على النوعCercopis vulnerata(Rossi, 1807) نموذجا للعائلة.وهذه ا | The present study comprisers taxonomic morphological of 15 species belong to four genera of the two subfamilies Cercopinae and Aphrophorinae, belong to the family Cercopidae of the order Homoptera, it has been regarded the speciesCercopis vulnerata(Rossi, 1807) as typical sample of the families.The species studied in were : Family : Cercopidae West wood, 1838Sub family : Cercopinae West wood, 1838.Genus : Cercopis Fabricius, 1775.**Cercopis vulnerata (Rossi, 1807)Sub family : Aphrophorinae Amyot&Serville, 1843.Genus : Aphrophora Germar, 1821.**Aphrophora alni(Falle'n, 1805).**Aphrophora major (Uhler, 1896).**Aphrophora oblique(Uhler, 1896).**Aphrophora salicina(Goeze, 1778)**Aphrophora straminae(Kato, 1932).**Aphrophora forneri(Haupt, 1919).**Aphrophora intermedia (Uhler, 1896).*Aphrophora triticasp.nov.*Aphrophora asadisp.nov.Genus : Phileanus Stal, 1864.Phileanus spumarius (Linnaeus) 1758.Genus : Neophileanus Haupt, 1935.** Neophileanus lineatus(Linnaeus, 1758).**Neophileanus campestris(Falle'n, 1805).**Neophileanus longiceps(Puton, 1895).**Neophileanus exclamationis(Thunberg, 1874). *New species for the science.**New recorded to the Iraq insect fauna of Homoptera.

التحري عن التشوهات الكروموسومية وطفرات المورث FLT3/ITD في مرضى ابيضاض الدم النخاعي الحاد == Screening For Chromosomal Aberrations And Gene Mutations FLT3/ITD In Patients With Acute Myeloid Leukemia

اسم المؤلف: سمارة كاظم محمد
اسم المشرف: عبد الحسين مويت الفيصل
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: صممت الدراسة الحالية من اجل تسليط الضوء على تاثيرات مرض ابيضاض الدم ((AMLفي بعض معايير الوراثة الخلوية والجزيئية الحاصلة في كريات الدم البيضاء في ثلاثة حالات للمرضى اشتملت على مرحلة ما قبل العلاج, في اثناء العلاج وبعد العلاج الكيميائي. كما شملت الدراسة ا | The present study was designed to shed light on the cytogenetic and molecular effects caused by acute myeloid leukemia (AML) pathogenesis in three stages before, during and after treatment with chemotherapy (in vitro) in lymphocytes. It was also aimed to investigate chromosomal aberrations, micronucleus formation and FLT3/ITD point mutations in CN - AML region 100 - 300 bp compared with healthy control. The study was comprised of forty - seven AML Iraqi patients their ages ranged between 2.5 - 81 years. It included twenty females and twenty seven males compared with twenty - six healthy control. The following results were obtained : AML is most common leukemia in adults and uncommon in children. It was found that 46.8% patients suffer from AML, who were under 15 years old, while 19.15% patients their age ranged between 16 - 30 years; 19.15% of patients their age were more than 45 years and 14.9% of patients their age ranged between 31 - 45 years of the total AML patients. AML is more common in males than females. The percentages of females and males were 42.5% and 57.5% respectively representing 1.35 : 1.00 male : female ratio. Two AML patients 5.3% have diploid cells when examined under light microscope. The highest mitotic index was 7.498±1.7 occurred in patients before taking treatment while recorded 6.784±4.5 during treatment and 7.000±2.5 after treatment. Nuclear anomalies such as nucleoplasmic bridges and nuclear buds were observed in AML patients, Micronucleus mean values recorded 0.033±0.018, 0.020±0.00015 and 0.036±0.01for AML patients before, during and after treatment respectively when compared with the control which recorded 0.002±0.00002. Nuclear division index (NDI) means before, during and after treatment of AML patients were 1.658±0.2, 1.000±0.022 and 1.424±0.19 respectively. Significant differences occurred among the three groups when compared with the control group (1.282±0.09). Extracted DNA from 30 AML patients was amplified by PCR to obtain FLT3/ITD gene from exon 11 to intron 12 and ten of them sent for sequencing. Two patients showed larger bands (470 and 460) bp when compared with wild type (330) bp. Among six patients, three displayed point mutations of deletion and substitution while others were normal since no mutations were detected. The percentages of mutation types were substitution 57.78% and deletion 22.2%. The effect of mutations was missense mutation (55.54%), deletion (22.22%) and nonsense (22.22%). It has been concluded from the current work that AML is more common in adult males, patients suffer from AML exhibited high MI, NDI, MN and point mutations are present in such patients including deletion and substitution causing missense, nonsense and deletion.
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