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تاثير بعض المستخلصات النباتية في معايير بيولوجية مختلفة للفئران == Effect of Some Plant Extracts On Different Biological Parameters On Mice

Author name: عقيل حيدر عطا الله
Supervisor name: مؤيد صبري شوكت
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: اجريت الدراسة الحالية للتحري عن المركبات النشطة الموجودة في المستخلص الخام المائي والميثانولي لاوراق الاس والنعناع والريحان ودراسة تاثيرها على فعالية انزيم الاسيتل كولين استريز ومستوى الدهون ومستوى السكر ومستوى انزيمات الكبد(ALP وSGPT وSGOT) ومستوى الانتر | The present study was conducted to investigate the active constituents found in aqueous and methanolic crude leaf extracts of Myrtus communis, Mentha piperita and Ocimum basilicum, and studies it effect on the acetylcholinesterase activity, levels of lipids, level of glucose, level of liver enzymes (ALP, SGPT and SGOT) and level of Interleukins (IL - 2, IL - 4, IL - 6 and IL - 10) in laboratory mice (in vivo). The results of the phytochemical analysis of the crude aqueous and methanolic extracts of Myrtus communis, Mentha piperita and Ocimum basilicum contain active compounds : Phenols, Flavonoids and Tannins and missing of Steroids and Coumarines in all extract but Saponins and Alkaloids found in alcoholic extract only, while terpens were present in Mentha piperita and Ocimum basilicum and absent in Myrtus communis. The results of administrating animals with different extracts showed no significant difference on blood Acetylcholinesterase (AchE) compared with ethanol liquid diet, while the alcoholic and aqueous extracts of M. communis, M. piperita and O. basilicum in the serum of decreased Acetylcholinesterase level significantly(p?0.05), liver and brain [(1.25 ?pH/30 min, 1.23 ?pH/30 min, 1.28 ?pH/30 min, 1.20 ?pH/30 min, 1.26 ?pH/30 min, 1.28 ?pH/30 min), (0.35 ?pH/30 min, 0.34 ?pH/30 min, 0.34?pH/30 min, 0.36?pH/30 min, 0.42?pH/30 min, 0.39?pH/30 min), (0.32?pH/30 min, 0.37?pH/30 min, 0.39?pH/30 min, 0.36?pH/30 min, 0.34?pH/30 min, 0.37?pH/30 min)] respectively compared with animals fed on ethanol liquid diet [(1.37 ?pH/30 min), (0.47 ?pH/30 min), (0.45 ?pH/30 min)] respectively. The methanolic and aqueous extracts of M. communis, M. piperita and O. basilicum reported a significant decrease in level of the total cholesterol and Low Density Lipoprotein (LDL) [(181mg/dl, 186mg/dl, 175mg/dl, 172mg/dl, 181mg/dl, 184mg/dl), (118mg/dl, 121mg/dl, 114mg/dl, 109mg/dl, 118mg/dl and 120mg/dl)] respectively, when compared with animals fed on ethanol liquid diet [(195 mg/dl), (132mg/dl) ] respectively while no effect was reported on High Density lipoprotein. The level of triglyceride reduced after the treatment with ethanol liquid diet, and then the level increased after the treatment with M. communis and O. basilicum extracts. The methanolic and aqueous extracts of M. communis, M. piperita and O. basilicum have led to a reduction in the level of glucose in the serum which increased after the treatment with ethanol liquid diet. Methanolic and aqueous extracts decreased the liver enzymes (ALP, SGPT and SGOT) significantly to the normal level (18, 17, 21, 18, 20 and 21) KAU, (19, 19, 16, 13, 17, 17) IU/L and (21, 24, 17, 15, 17 and 19) IU/L respectively after the increase by the treatment with ethanol liquid diet (24) KAU, (26) IU/L, (28) IU/L as compared to control (20) KAU, (12.2) IU/L, (13.5) IU/L, respectively. The level of Interleukin - 2 and Interleukin - 4 in the serum significantly increased in the treatment of alcoholic and aqueous extract of M. communis, M. piperita and O. basilicum [(18 pg /ml, 17 pg /ml, 20 pg /ml, 18 pg /ml, 20 pg /ml, 20 pg /ml), (100 pg/ml, 110 pg/ml, 119 pg/ml, 108 pg/ml, 90 pg/ml, 92pg/ml)] in comparison with ethanol liquid diet treatment [(14 pg /ml), (77 pg/ml)] respectively. While the level of Interleukin - 6 and Interleukin - 10 increased significantly in the serum, when animals were fed with ethanol liquid diet [(259pg/ml) and (501pg/ml)] respectively, and then decreased significantly after the treatment of methanolic, aqueous extract of M. communis, M. piperita and O. basilicum reported [(198 pg/ml, 202pg/ml, 202pg/ml, 201pg/ml, 214pg/ml, 217pg/ml), (370 pg/ml, 385pg/ml, 200pg/ml, 280pg/ml, 350pg/ml and 350pg/ml)] respectively.

التاثيرات المعدلة - مناعيا للمعززات الحيويه ضد بكتريا Salmonella enterica Serovar Typhimurium المعزولة من حالات الاسهال لدى الاطفال في ذكور الفار الابيض == Immunomodulatory Effects of Probiotics On Salmonella Enterica Serovar Typhimurium Isolated From Diarrheal Children In Albino Male Mice

Author name: صفا خليل ابراهيم
Supervisor name: عبد الواحد باقر الشيباني | علي حسين ادحيه
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: This study aimed to determine the frequency of Salmonella Typhimurium and its multi - drug resistance (MDR) status as a diarrheal causative pathogen in children younger than five years old, as well as to investigate the role of four probiotic microorganisms(L.acidophillus, L.casei, S.cerevisiae, S.boulardi) in controlling such infection.For this purpose, 128 stool samples of patients (76 males and 52 females) was included in this study. They were children suffering from diarrhea and fever who admitted to the “Central Pediatric Hospital” in Baghdad during the period from April to September, 2012. Their ages ranged from six days up to five years.The immunological effects of probiotics and MDR S. Typhimurium isolate in the experimentally infected and probiotic - treated mice were investigated via assessing the level of ten cytokines (IFN - ?, IL - 1?, IL - 4, IL - 10, IL - 12, IL - 17A, IL - 21, GM - CSF, RANTES and IP - 10) in the lavage of small intestine. Accordingly, five groups of mice were used in the in vivo part of this study; Mice in group I received probiotic for 7 successive days, challenged with S. Typhimurium on day 8, and dissected on days 14 and 21. Group II wassimilarly treated, but the probiotic was continued for 14 days. Group III was given the probiotic only, and group IV was challenged with S. Typhimurium, while group V was the control.Results obtained in this study could be summarized as follows : 1. Out of 128 stool samples, S. Typhimurium was isolated and identified in 9 samples only (7.03%). All isolates were totally resistant to nalidixic acid, with the exception of one isolate which showed intermediate sensitivity.Furthermore, only one of these isolates (symbolized B) was found to be resistant to three antibiotics (ampicillin, amoxicillin and nalidixic acid).Therefore, this isolate was considered as an MDR isolate and selected for further experiments in the study.2. When the susceptibility of MDR S. Typhimurium isolate B was further assessed in vitro by using four probiotics (Saccharomyces cerevisiae, S. boulardi, Lactobacillus acidophilus and L. casei), results showed that S.cerevisiae and L. acidophilus were the most efficient by recording the highest inhibition zones (12.6 and 16.3 mm, respectively), therefore, they were further investigated for their anti - S. Typhimurium effects in vitro and in vivo. 3. By using the unconcentrated and (one - fold, two - fold, three - fold) concentrated filtrates of these two probiotics, it was found that the three - fold filtrates were most efficient in their antibacterial activity by recording the highest inhibition zones (25.0 mm for S. cerevisiae and 31.0 mm for L. acidophilus.4. Significant increases in the values of liver index were observed in mice of group I treated with L. acidophilus at 21 days (10.73%) compared to the corresponding group treated with S. cerevisiae (7.41%) or other four groups. For spleen, index value in L. acidophilus groups was higher than the corresponding groups in S. cerevisiae. Mice treated with a probiotic alone or in a combination with the pathogen showed significant increases in the spleen index values of all groups compared to the untreated mice (group V).5. Both probiotics were effective in reducing S. Typhimurium colony forming units per plate (cfu/plate) in the liver and spleen. In liver, mice in group IV showed a count of 224.4 cfu/plate, which was significantly higher than any count in groups of L. acidophilus and S. cerevisiae. Group II mice recorded better results than group I mice, while the lowest counts (21.6 and 27.8 cfu/plate for L. acidophilus and S. cerevisiae, respectively), were observed at day 21.6. The ten investigated cytokines showed different levels in the small intestine wash; such differences were subjected to the group of mice underinvestigation and type of probiotics used. In addition, variations in IFN - ?/IL - 4, IFN - ?/IL - 10, IL - 4/IL - 10 and IL - 17A/IL - 10 were also observed.

دراسة تصنيفية حياتية مقارنة لمراتب من الجنس Onobrychis Mill. (Fabaceae) في العراق

Author name: زينب جواد عامر
Supervisor name: عذية ناهي سلمان المشهداني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: استــهدف البــحث الــحالي دراســة تصــنــيفية مقارنة لاحدى عشر مرتبة تصنيفية تابعة للجنس Onobrychis Mill. من العائلة الفراشية (البقولية) Papilionaceae النامية بريا في العراق وهي O. acaulis وO. caput - galli وO. carduchorum وO. crista - galli بضربيه O. cri | A comparative systematic study was carried out for eleven taxa belong to the genus Onobrychis Mill., Family Papilionaceae grown widely in Iraq, and they are O. acaulis, O. caput - galli, O. carduchorum, O. crista - galli with two varieties O. crista - galli var. crista - galli and O. crista - galli var. trilophocarpa, O. galegifolia, O. haussknechtii, O. kotschyana, O. megataphros, O. ptolemaica, O. schahuensis. The Study included the gross general Morphological characters and Micromorphological characters, also the Pollen grains studied and Anatomy, Chemistry, Enviroment and Geographical distribution and Taxonomic treatment, also botanical key was putted to separate the taxa. General survey for the Phytogeographical districts where the taxa of genus distributed, in order to detect the distribution of the studied taxa of the genus Onobrychis. Therefore many collections obtained and new locations of taxa distribution were recorded, the specimens studied, and correct the mistake naming specimens were identified and reidentified some miss identified specimens. A comparative morphological study was undertaken to all taxa plants parts include the root, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying, with natural images for different morphology characteristics, as well as the field images. This study indicates that the characters of Leaf, Inflorescence, Flowers and Fruit were collectively contributed in the separation and identification of taxa. The pollen grains have a taxonomic importance because their different shape, dimensions of polar and equilateral view and wall thickness. The anatomical study showed the importance of Taxonomic support to the morphological characters in the diagnosis and isolation different taxa, and this study showed different in the anatomical characters of leaf include the characters of Epidermis, Stomata and the characters of a cross sections in stems. The current study showed that the trichomes were very important taxonomic in isolate different taxa because their variation in density, dimensions and the shape of epidermis cells that the trichomes arise from them and dimensions of the epidermal cells and the nature of their walls. The current study identified nineteen protein - amino acids by using High - Performance Liquid Chromatography (HPLC) and the distribution of these compound was variable among various taxa of the genus and that was useful in isolation different taxa. and the aliphatic amino acids, Glutamic acid, Glycine, Serine, Arginine, Alanine, Tyrosine, Valine, Methionine, Isoleucine and Lysine had a wide presence in the studied taxa, which means that they belong to the same genus. The ecology and geographic distribution of the taxa were studied using area survey and information of herbaria specimens, maps have been drawn for taxa distribution in the Iraqi natural geographical provinces. At last, a taxonomic treatment has been carried out for the taxa of the genus Onobrychis, as well as a detailed key to isolate them.

دراسة عدد من ملوثات الهواء وتاثيراتها البيئية في منطقتين مختارة من جانب الرصافة في بغداد == Study of Several Air Pollutants And Their Environmental Effects In Two Regions In Al - Rusafa Site In Baghdad

Author name: شيماء هادي حمادي الدليمي
Supervisor name: محمد نافع علي العزاوي
General topic: Biology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: Several air pollutant concentrations such as Suspended Particulate Matter (SPM), carbon oxides (COx), sulfur dioxide (SO2), nitrogen oxides (NOx), methane (CH4), ozone (O3) and some heavy metals such as lead (Pb) , chromium (Cr), cadmium (Cd), and nickel (Ni). Were measured in selected areas of the Rusafa in Baghdad, Anda’lus square (as a commercial site) and Wazeria district (as industrial site).The results showed that the concentration of air pollutants from gases, SPM (NO2, CO, SO2, and SPM) and heavy metals (Pb, Cr, Cd, and Ni) were exceeded globally and locally permissible limits in both study sites, while the concentration of O3, CO2 were within the globally and locally standard limits in both study sites. Commercial site had recorded higher concentrations of (CO2, SPM, Pb) than those of industrial site. While the industrial site had more pollution with (NO, NO2, CO, CH4, Cr). Results showed a relationship between climate factors and air pollutants, where windspeed, temperature, and relative humidity had influenced on the concentration of air pollutants.Median Lethal Concentration (LC50) to nitrogen dioxide gas was identified, by exposing a community of 30 adult male mice. The mice weights were between 23 - 31gm and their age was 8 - 12 weeks. The mice were randomly divided into five groups (6 male of each group). The exposure periods were 20, 40, 60, 80, 100 minutes using different concentrations 50, 100, 150, 200 ppm.The results showed a higher mortality during 150 and 200 ppm concentrations, for exposure periods of 80 and 100 minutes, but other concentrations have not been recorded. LC50 was calculated 161.86 ppm for 80 minutes and 110.53 ppm for 100 minutes.

دراسة التاثيرات الحادة والمزمنة للمبيد العشبي - 4, 2 دايكلوروفينوكسي اسيتك اسد في نوعين من اسماك الكارب == Study The Acute And Chronic Effects of The Herbicide 2, 4 - Dichlorophenoxy Acetic Acid In Two Species of Carp Fish

Author name: ضحى زكي السويفي
Supervisor name: احمد جاسم محمد العزاوي
General topic: Biology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: تضمنت الدراسة الحالية التاثيرات السمية الحادة والمزمنة لمبيد 2, 4 - d في نوعين من اسماك الكارب (الاعتيادي والعشبي)، استعمل في دراسة التاثيرات الحادة عدة لكلا النوعين قيد الدراسة وكانت ((170, 165, 160, 155, 145, 140, 130 و(140, 130, 115, 95, 70)ملغم/لتر ع | This study included acute and chronic effects of 2, 4 - d pesticide in two species of carp fish (Cyprinus carpio and Ctenopharyngodon idella) using several concentrations for the two carp fish species that were (130, 140, 145, 155, 160, 165, 170) and (70, 95, 115, 130, 140) mg/l respectively for 96 hours, to determine the median lethal concentration (LC50) and the behavioral changes was used as a standard of those effects. Whereas three concentrations (20, 40 and 60) and (10, 15, 25) mg/l respectively for the two carp species were used in the chronic exposure for six weeks. The behavioral, histological and hematological changes were used as a standard to observe these effects through the study period. The value of LC50 for common carp fish were 157.77, 157.25, 156.41 and 152.62 mg/l. For grass carp, LC50 were 115.92, 114.92, 112.94 and 96.52 mg/l for 24, 48, 72, 96 hrs of exposure to 2, 4 - d pesticide concentrations respectively.Behavioral changes were recorded more effects in high concentrations than in low concentrations. To discover the toxic effects of this substance on fish, many parameters were used. They included the clinical symptoms by erratic swimming, hyperactivity, loss of equilibrium with respiratory effects such as rapid gill movement and swam near the water surface. While in chronic effects, blood parameters were changed in Red Blood Cell R.B.C., White Blood Cell W.B.C., Packed Cell Volume P.C.V., Haemoglobin Hb and liver enzyme (GPT & GOT).Values elevated significantly according to the pesticide concentration (P?0.001) and exposure periods (P?0.05) in comparison with control. The histological examination of the gills, liver and kidney of the common and grass carp fish after chronic exposure to 2, 4 - d herbicide showed pathological changes and alterations such as fusion of the secondary lamella, epithelial lifting, blood congestion, epithelial hypertrophy of the lamellar epithelium and epithelial necrosis. In the liver, pathological changes were seen, including hepatocytes hypertrophy, cytoplasmic vacuolation, blood congestion and cellular necrosis. Degenerative changes of the renal epithelium, necrosis in renal tubules and occlusion of the tubular lumen where the pathological alterations in the kidney.

انتشار داء المقوسات العيني مع تقييم لبعض جوانب الحالة المناعية في عينة من المرضى العراقيين == Prevalence of Ocular Toxoplasmosis And Evaluation of Some Immune Status Aspects In A Sample of Iraqi Patients

Author name: عبير فاروق ساكن الحكيم
Supervisor name: اسراء قاسم العبيدي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت الدراسة الحالية على 104 مريضا من المراجعين لوحدة الشبكية في مستشفى ابن الهيثم التعليمي للعيون في بغداد للمدة من بداية شهر كانون الاول 2013 لغاية شهر حزيران 2014. قسمت مجموعة المرضى استنادا للتشخيص السريري وفحص اختبار التلازن Latex test الى 50 مصابا | The current study was conducted on 104 patients whose underwent to the retina unit in Ibn Al - Haitham Hospital of Eyes in Baghdad from December 2013 to May 2014. The patients groups were divided according to the clinical diagnosis to 50 patients infected with ocular toxoplasmosis (OT) and 54 patents with uveitis, in addition to these groups acontrol group was also included 96 healthy apparent persons without any infection in their eyes.Some epidemiological characters were studied like age, that showed a little effect on two groups OT (Mean of age 38.07 ± 15.14) and uveitis (Mean of age 35.1 ± 17.6). The two groups were found in range 20 - 40 year while the control group include individuals with different age due to the randomly collection. The occupation factor play arole in the prevalence of infection with highly significant differences (P<0.05) in (house wife, workers) with a percentages 35(70%) 35(64.8%) in OT and uveitis groups respectively but the control group expend to include house wife, workers and officers with a total rate 92.7%.The education level also showed significant differences (P<0.01) among the studied groups, the most of ocular toxoplasmosis and uveitis samples were found in highly frequency at the primary, intermediate and secondary educational level with total rate 74% and 77.81% respectively while the control group was found at the secondary and under graduate educational level in 77.1%. The residency character was also examined and no revealed significant difference (P<0.05).The infection path was also set by the presence of animals especially cats which ware existed leading to statistical differences, p? 0.01 for OT group with rate 18%. The visual activity character showed no significant difference (P?0.05) between the patients of OT and uveitis who affected their visual acuity in right eye while the doubling response was recorded for uveitis patients with rate 55.6% in comparable with OT patients with rate 38% for the left eye. The intra ocular pressure was normal in both groups and for both eyes without significant difference.Latex and ELISA IgG. IgM serological tests were used in this study. The results were showed that latex test is easier and it is appear that OT patients were diagnosis with 100%, while the uveitis patients didn't show any positive result. Control group showed that 21 individuals were infected with rate of 21.9%.ELISA - IgG toxo. antibodies confirmed that the infection rate of OT patients was 39 (78%) versus 11(22%) with negative result, while the study also confirm that uveitis patient that have OT disease was 12 patient from 54 case, 42 case didn't diagnosis for OT. The control group was re - evaluated showed 28 case with toxoplasmosis infection from 96 case which from the subclinical group. The antibodies levels were measure by international unit/ ml for all groups, the IgG level was 3.186 ± 1.703 Iu/ml in positive control group followed by OT patients with 2.927 ± 2.417 and uveitis group 0.637 ± 0.803, finally the negative control group 0.276 ± 0.163. IgM levels were low and its presence didn't have any positive case. This study also showed that there was a relation between the IgG level and the pathological effect on eyes which resulted from infection for both groups OT and uveitis, it was high rate 73.5% with 50 patient, for posterior uveitis causing significant differences (P<0.05) followed by anterior uveitis with rate 13.2%.As for the results of cytokines there was a high level of IFN - ? in positive control group 793.7 ± 3461.6 pg/ml followed by OT group 550.6 ± 210.6 pg/ml, the uveitis group that in infected with ocular toxoplasmosis and negative control recorded 524.1 ± 173.6 and 506.6 ± 104.6 pg/ml levels respectively, this rise in the level of IFN - ? cause a significant differences at (P<0.05) for OT, uveitis (non infected) and positive control groups. TNF - ? recorded arise of its level in positive control group 34.89 ± 12.29 pg/ml followed by OT group 32.24 ± 9.91 pg/ml then positive uveitis group 23.61 ± 12.91 pg/ml after them negative control 14.62 ± 5.43 pg/ml finally negative uveitis group with 7.76 ± 4.04 pg/ml.High level of 1L - 12 was gotin positive control group in a concentration 827.00 ± 224.25 pg/ml followed by OT group with 811.28 ± 179.40 pg/ml. An increase in 1L - 17A was recorded in positive control group with mean 327.65 ± 77.89 pg/ml while converged concentration in OT group 263.73 ± 47.48 and positive uveitis group 252.57 ± 61.55 pg/ml. The lowest level was in negative control group 421.6 ± 168.14 pg/ml. MCP - 1 showed a rise in its level in positive control group 274.09 ± 92.05 pg/ml and reduce in OT patients 217.68 ± 98.70 pg/ml, while the two groups negative and positive uveitis recorded levels 155.71 ± 99.51 and 132.60 ± 99.02 pg/ml respectively. The lowest level was register in negative control 97.56 ± 79.17 pg/ml.

العلاقة بين متلازمة تكيس المبايض وهرمون المضاد لمولر وبرامج تحريض الاباضة عند اجراء عملية التمنية داخل الرحم

Author name: عبير ناظم جاسم
Supervisor name: محمد باقر محمد رشاد فخر الدين | صباح مهدي حسين
General topic: Biology
Specific topic: Biotechnologies
Degree: Higher Diploma
Language: English
University location: Baghdad
First pages:
Abstract: تعد متلازمة المبيض متعدد الا كياس هي من اكثر امراض الغدد الصماء شيوعا عند النساء حيث يصاب من النساء في سن الانجاب مايقارب (5 - 10%). الاعراض الرئيسيه لمرض متلازمة المبيض متعدد الاكياس هو اضطراب الدوره الشهريه مع فشل لاباضه وظهور اعراض زيادة الهرمون الذكر

التحري الجزيئي عن بعض التغيرات في الدنا المايتوكونديري للنطف لمرضى يعانون من وهن حركة النطف == Molecular Screening of Some Changes In Sperm Mitochondrial DNA In Asthenozoospermic Patients

Author name: عدي عدنان مهدي
Supervisor name: اسماعيل عبد الرضا عبد الحسن
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: من خلال البحوث والدراسات التي اجريت على اسباب وهن حركة النطف لدى الرجال الا انه هناك عدد من المسببات لم تحدد والى الوقت الحاضر. الا انه بالرغم من ذلك بعض الدراسات تشيربان العامل الوراثي يلعب دور في ذلك والمتمثل بعضيات المايتوكوندريا والحاوية على الدنا الم | Through researches and studies done on the causes of the asthenozoospermia in men, although there was no specific reason so far responsible for the infertility in people, but some of the studies were indicated that the genetic factors plays a role in the sperm dysmotility through the mitochondria that contained mitochondrial DNA responsible for providing the energy required for the sperm motility by production of ATP, which serve as the fuel that is consumed by sperm during motility. This study is conducted to determine the changes that could occur in the sperm mtDNA which included (common deletion, mtDNA copy number per cell and mutations in the ATPase8 and ATPase6 genes).This study was consisted of two parts as follow : The first part was consisted of two steps : The first step conducted on 71 samples from subjects suffering from the weakness of the movement of sperm and 12 samples from subjects who have a normal movement of sperm, total samples were 83 samples were divided patient samples into 5 groups depending on the percentage of the sperm motility under the classification adopted by the World Health Organization as well as a control group, that it has become 6 groups, these samples were subjected for investigation about the mitochondrial DNA deletions and mitochondrial DNA copy number by using real time PCR.On the other hand was taking 66 sample dispersed sample 56 patients and 10 healthy samples were subjected to the tests of ATPase6 and ATPase8 genes sequences for detection about mtDNA mutation.The second step was included the use of discontinuous gradient centrifugation (percoll) , which are 40 % and 80 % which represent both progressive and non progressive motile sperms, bringing the total samples 166 samples that collected and fractionated in a hospital, Kamal Al - Samarrai to treat infertility and IVF in Baghdad and examined for further molecular investigations for the period from February 2012 to October 2013.The second part was included the molecular tests that carried out after DNA extraction from the cells and this part include three steps : The first step was to investigate the common deletion in mtDNA where the results showed that the total number of deleted samples in the group (0 - 5) in the nonprogressive sperm was 81.25 %, which ranged between 6.8 - 74.7% while the ratio between the other groups 6 - 10, 11 - 15 , 16 - 20, 21 - 25 where were 75, 35.72, 28.5 , 36.3, and 12% for control group respectively. The second step was to investigate the sperm mtDNA copy number. The same groups where the results showed that the content of DNA in subjects with impaired movement of sperm was high compared with the control group where scored in some samples the highest level of gene expression 246.9 copies per cell in the group (0 - 5%) either other groups have shown lower levels were observed in groups (6 - 10) , (11 - 15) , (16 - 20) and (21 - 25) were 203.1, 180.7, 133.5 and 128.3 copies per cell, either the highest level of DNA content of the control group was 94.7 copies per cell, there was also a significant difference (P>0.05) between the normospermic motile isolated from 80% class and poor sperm movement isolated from 40 % class. The third step was included the detection of mutations by analyzing the sequences of the MT - ATAase8 and MT - ATPase6 genes which documented 59 mutations that were 23 missense and 36 silent mutations where all of them were heteroplasmic mutation except for a single mutation of the type missense (A8860G) which was homoplasmic mutation was noticed in all mtDNA copies of patients and control subjects also in progressive and non progressive sperm cells. Thought this current study novel nucleotides changes in MT - ATAase8 and MT - ATPase6 genes among groups where 3 novel missense mutations in MT - ATAase8 gene at positions (8378, 8483 and 8558), the changes of nucleotides bases were A>G, A>C, A>C and A>C respectively, replacing Asparagine to Aspartic acid, Leucine to Proline and Proline to Alanine respectively. Also two novel missense mutations were observed in MT - ATPase6 gene at positions nt (8822 and 9055) where the changes nucleotides were (C >T and G>A) that replaced amino acids (Serine to Phenylalanine and Alanine to Threonine) respectively. Silent novel heteroplasmic change as a transition substitutions in ATPase8 gene at position nt (8371) by replacing C>T without any changing in amino acid of protein. Results of this present study showed novel heteroplasmic silent mutations in ATPase6 gene at positions nt (8899, 9048 and 9060) nucleotides changes (C>T, T>C and C>A) respectively, without changing in amino acid of protein which were observed in infertile group

تشخيص بكتريا Neisseria gonorrhoeae بالطرق التقليدية والجزيئية في المرضى الذكور ودراسة مدى تاثيرها في حدوث الحذوف في موقع AZF == Conventional And Molecular Diagnosis of Neisseria Gonorrhoeae In Male Patients And Study Its Suspected Effects In Microdeletions In Azf Locus

Author name: غزوان علي مسلم الرماحي
Supervisor name: عبد الرضا عبد الحسن اللامي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: This study includes 82 clinical samples from male patients suspected to have gonorrhea (urethral discharge with dysuria). Two types of samples were collected from each patient, urethra swab and blood samples from patients who attended Al - Yarmouk teaching hospital in Baghdad city, Clinics and private laboratories during a period from December 2012 to April 2013. All of them were married and have children's, and 20 samples were taken from apparently healthy subjects.Cases caused by gonococci were diagnosed by finding the Neisseria gonorrhoeae bacteria in the samples using microscopic examination and culturing on rich media chocolate agare for growth bacteria and selective media modified Thayer martin agar (MTM) contact antibiotic to prevent growth of all the types of bacteria except N. gonorrhoeae.Microscopic examination using specific kit for gram stain, showed gram negative diplococcus, like bean shape. Culturing on rich media revealed that 82 (100%) samples positive but re culturing on Modified Thayer martin media revealed that 76 (92.68%) out of 82 positive. Then biochemical test had the same results.Results of the molecular diagnosis of the bacteria causing gonorrhea using specific primers that were specific for the Orf1 gene, revealed that 80.26% of samples (61 out of 76) were positive. Results of the microdeletion in Y chromosome AZFc region revealed that no microdeletion were occurred in SY - 254 STS and BPY - 2 gene.This study, provided high specificity and sensitivity for the diagnosis of gonorrhea using PCR technique which is cheaper and faster than traditional methods. Also, PCR - based method for detection of N.gonorrhoeae can be readily used in hospitals and laboratories.

الكشف عن السمية الوراثية لمستخلص نبات الصبار Aloe vera باستعمال المؤشرات الجزيئية والخلوية == Genotoxic Effect of Aloe Vera Extract Using Molecular And Cytogenetic Markers

Author name: غيث منذر فاضل
Supervisor name: نضال نعمة حسين
General topic: Biology
Specific topic: Plant - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: نبات الصبار Aloe vera L. من النباتات المهمة والتي تستعمل حاليا بشكل واسع في مجال صناعة الادوية ويضاف هلام الصبار الى اغلب مستحضرات التجميل مع بعض المواد الغذائية في جميع انحاء العالم, لذا تم دراسة التاثيرات السمية الخلوية والوراثية لتراكيز مختلفة من المس | Aloe vera L. is one of the important plants in the world.It is currently and widely used in pharmaceutical industry, and its gel in some time added to some foods all over the world. Therefore, the present study was conducted to evaluate the cytotoxic and genetic effects of crude, alcohol and aqueous extracts of Aloe vera gel toward the onion(Allium cepa L.) roots (as a biological system) exposure at different periods. Onions roots were treated with different concentrations of the extracts : 2% , 5%, 10%, 20% and 40% (v/v)of the crud extract , 5%, 10%, 20% 30% and 50% (v/w) of alcohol extract and the concentration 25%, 50%, 100%, 150% and 200%(v/w) of aqueous extract of Aloe vera gel exposed for different period (24, 48 and 72) hour. The investigation were focused on the effect of these extracts on onions roots length rate and some cellular properties (mitotic index, phase index, the percentage and type of chromosome aberrations, as well as a genetic study using Random Amplified Polymorphic DNA (RAPD) of onions roots treated with different concentration of this extracts. Results showed that all Aloe vera gel extracts had inhibit activity toward onion roots length rate. The inhibition associated positively with the increasing concentration. The effective concentration (EC50%) were 10% of the crude extract, 20% of the alcoholic extract and 100% of aqueous extract, indicating the effectiveness of the crude extract followed by alcoholic extract and then the aqueous extract. Cytological study showed that all Aloe vera gel extract revealed a significant decrease in mitotic index (MI%) of the onion roots cells compared with the control group, and the effect was positively associated with the increasing concentrations of the extract. However, the effect was independent on the exposure period. The concentrations 10%, 20% and 150% of the crude, alcohol and aqueous extract respectively indicated a reduction of the mitotic index to almost 50% of the control treatment. Therefore, this concentrations considered as sub - lethal concentrations for this extracts respectively while the higher concentrations than 30%, 50% and 200% of the crude, alcohol and aqueous extract which reduced the mitotic index to almost 22% of the control treatment were considered as lethal concentrations. The results also showed a significant decreases in prophase and an increase in metaphase in onion roots cells treated with extracts comparison with control treatment. Many of chromosomal abnormalities were appeared due to the treatments of the onion roots with different concentration of the extracts, the percent abnormalities was increased with the increasing concentration of the extract and exposure period. The most frequent of chromosome abnormalities were chromosomal stickiness, disturbed chromosome, chromosomal bridges, C - mitosis, vagrant chromosomes in addition to the other chromosomal abnormalities star telophase. The toxicity effect of Aloe vera gel were studded on molecular level by using random amplification polymorphism of DNA(RAPD). Ten random primers were used, seven of them gave polymorphic bands in all the studied samples and their molecular weights were ranging from 100 to 1600 base pair. The results of Random Amplified Polymorphic DNA (RAPD) showed significant difference in the number of DNA band and their molecular weight of the onion roots treated with Aloe vera extracts compare to control treatment. The high concentrations(higher than EC50%) of extracts were showed to be more effective on the DNA through the appearance or disappearance of larger number of DNA band in comparison with control. The genetic template stability (% GTS) was significantly decreased in DNA of onions root treated with high concentrations (higher than EC50%) of Aloe vera extracts, therefore the high concentrations of Aloe Vera extracts were considered to be genetically toxic. The genetic dendrogram was obtained using Jaccard factor of genetic divergence and the result showed segregation of the samples treated with heights concentrations (%40 200% and 50%) of crude, alcohol and aqueous extract from other samples and control treatment. Therefore, the RAPD method can be considered as an efficient method in detecting for assessment of genotoxicity of crude, alcohol and aqueous extract of Aloe vera gel.

التفكك الحياتي لمبيدي الملاثيون والدورسبان باستخدام مزارع احاديه وخليطه من البكتيريا == Biodegradation of Malathion And Dursban By Mono And Mixed Bacterial Cultures

Author name: فائزة كاظم عمران
Supervisor name: عادل مشعان ربيع
General topic: Biology
Specific topic: Environment
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: عزلت 45 عزلة بكتيرية محليه من مجموع 30 نموذج تربه ملوثة بالمبيدات والتي جمعت خلال فترات زمنيه مختلفة ومن مناطق مختلفه في بغداد. اختبرت قابلية العزلات البكتيريه على تفكيك مبيدي الملاثيون والدورسبان. اجريت غربلة اولية وثانوية باستخدام وسط الاملاح المعدنية ا | Forty five local bacterial isolates were isolated from thirty different soil sample contaminated with pesticide, These samples were collected from different Baghdad regions at different periods. The bacterial isolates were tested for their ability to biodegrade certain pesticides (Malathion and Dursban). Primary and secondary screenings were carried out using solid Mineral Salts Medium (MSM) and Liquid (MSM) with 100 ppm Malathion and Dursban at 37 0C, pH 7. Twenty seven isolates resistant to Malathion and Dursban were isolated. Out of 27 bacterial isolates from different locations in Baghdad. Results from primary screening showed that the 10 isolates had good growth, 5 isolates gave moderate growth, 12 showed weak growth and only 9 isolates failed to show any growth.According to current results, 10 bacterial isolates were tested for their ability to biodegradation of Malathion and Dursban in liquid medium, However, secondary screenings results by using minimal inhibition concentration(MIC) revealed that only four bacterial isolates had the highest ability to degrade Malathion and Dursban and subsequently were identified as Pseudomonas putida, Esherichia coli, Escherichia hermanii and Staphylococcus vitulinus by Vitek compact system respectively.The optimum conditions (incubation period, pH, temperature) for growth and biodegradation of Malathion and Dursban were examined. The obtained results indicated that the best incubation period was after 7 days, pH =6 and favorite temperature was 350C.Measurement physicochemical properties for soil pH, temperature (C°), electrical conductivity EC (µs/cm) for both Dursban and Malathion concentrations(ppm) collected from six different locations within Baghdad city. Biodegradation of Malathion and Dursban in soil inoculated with different bacterial mono - cultures and mixed - cultures were investigated. soil pH, temperature, electrical conductivity, cations and anions, on the population of native Malathion - Dursban tolerant bacteria in the cultivated soils of six sites. The result showed that soil physical properties like temperature and electrical conductivity affect significantly to native Malathion - Dursban tolerant bacterial density. Similarly, the soil chemical properties like pH, cations and anions had more effect on the abundance of Malathion - Dursban tolerant bacteria in the soil. However, the impact of soil organic carbon, organic nitrogen and available phosphorus was very significant. The results of the present study can be utilized for the development of effective bioremediation process for pesticide - contaminated soil. Under optimal conditions, Malathion and Dursban degradation was measured by using GC - MS analysis. The results revealed that Pseudomonas putida was the best isolate for degrading Dursban by monoculture isolate (47.18%) , while mixed culture(P.putida and S.vitulinus) was the best in degrading Malathion and Dursban that gave 90.84% and 81.055% respectively. Results also indicated that the number of byproducts produced by mono and mixed culture of Malathion and Dursban biodegradation. Whereas the results obtained from GC/Ms analysis revealed that not detected of toxic byproduct Malathion and Dursban degradation by Pseudomonas putida and Staphylococcus vitulins respectively.Moreover, under natural conditions.The study tested the ability of selected bacterial isolates (P. putida and S. vitulins) in biodegradation of Malathion and Dursban, the results showed slower biodegradation by selected bacterial isolates compared with laboratory conditions but study showed a intermediet product like phenol, diethyl phosphorothioate and oxon.The GC - MS results showed that the number of metabolic intermediates formed during the degradation of Malathion by bacterial mixed - culture were relatively higher in number than by bacterial mono - culture.The mass spectra of malathion containing samples treated with bacterial mono and mixed culture showed no any known toxic intermediates. The result indicated that the two main degradation products resulted from bacterial degradation, namely malathion monocarboxylic (MMA) and malathion dicarboxylic acid (MDA), the first one may convert into the latter over time. Some other degradation products may occur such as ethyl hydrogen fumarate (EHF) but in negligible amount.The result indicated that the main degradation products resulted from bacterial degradation of dursban is trichloropyridinol(TCP) and then de - chlorinated into 2 - pyridinol.The mass spectra results obtained in this study showed that the dursban was degraded to many metabolites.The results suggested that the bacterial isolates were not forming any toxic intermediates but no any intermediate was identified till R.T. 22 minutes. This indicated that dursban is probably completely metabolized by the isolates into smaller intermediatesThe isolate Pseudomonas putida isolated from soil contaminated with pesticides was identified genotypic tests. Molecular typing was performed by RAPD - PCR and comparison of the results to other Pseudomonas isolates. The result shows that genotyping differences between isolates and found the convergent percent biodegradation between this isolates of both selected pesticide, although from these differences, there was no more effect to biodegradation, which means that the biodegradation of Malathion and Dursban not related to genotype

دراسة امكانية الاستفادة من تركيز بروتين المصل الفعال نوع سي بروتين التفاعلي CRP في التشخيص المبكر للاصابات البكتيرية لحديثي الولادة وتحديد توقف اعطاء العلاج من خلاله == The Study of Usefulness of C - Reactive Protein Concentration In The Early Diagnosis And Determining The Duration of Antibiotic Therapy of Suspected Neonatal Bacterial Infection

Author name: قاسم ابراهيم حسين
Supervisor name: ايمان ناطق ناجي البياتي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: ان التشخيص السريع لاصابات تجرثم الدم في الاطفال حديثي الولادة من الامور المهمة وذلك لخطورة الاصابة المؤدية الى الموت.الغرض من دراستنا هو امكانية استخدام فحص سي - البروتين الفعال في التشخيص المبكرلهذا المرض البكتيري وكذلك استخدامه في تحديد نقطة التوقف عن ا | Rapid diagnosis of bacterial infections of newborn infants is very important itcause of morbidity and mortality. Aim of the studyThe aim of the studyis to examin the possibility of using C - reactive protein in the early diagnosis of neonatal sepsis, as well as its use in determining thestop point of the antibiotic treatment.Patent, Materials and methods This study was conducted at the Hospital of Ibn - Albaladi for children and women in Rusafathe Healthoffice / Baghdad.Iraq from February 2013 to February 2014.The number of patients with bacterial infection was 102 (62%) of the total number of patients (165) who were admitted to the neonatal care unit aged less than 28 days and their weights was more than 1500 g,. The number of males was 92 (56%) and the number of females was 73 (44%), the number of patient withearly onset sepsis EOS (less than three days) was 43 (42%) and with late onset sepsis LOS for (more than three days) was 59 (58%), 43 (57%) ofEOSwas normal deliveries infected and cesarean deliveries infected 33 (43%), while51 (57%) of LOS was normal deliveries infected and cesarean deliveries in LOS was 38 (43%). Blood samples were collected and divided into three portion : bloodculture, CRP andhematological profile.1 - Blood culture.Blood was collection from neonatal sepsis patientinto special blood culture bottle and incubate in bact /Alert 3 D device when growth of bacteria then subcultured on blood agar, MaCconkey agar, and incubatedusingvitek /2 to diagnose bacterial infection anddeterminetheir susceptibility to antibiotic treatment.ResultThe Compact results indicated that 63 (38%)of patient were negative and 102 (62%) were positive out of them 23 patients (22.6%)were infected with gram negative bacteria including {Klebsiella pneumonia 30%, Acinetobacterlowffi 17%, Enterobacter cloaca 13%, } while 79 (77.4%) were infected with gram positive bacteria including { staph Homins and staph hemolyticus each 28% then staph epidermidis 13% and staph aureus 10%, while strep spp was 6% }. 2 - c - reactive protein.It was done by twomethod for the same samples in order to characterize the accuracy of the diagnosis. The first method was agglutination test (method 1) the cut off value for positive result ? 6 µg % 79 (48%)were negative, while 86 (52%) werepositive, while the second method was immune test (method 2) µµµusing Nycocard device werethe cut off value for positive result ? 5 µg % the results 102 (62%) were positive while 63 (38% negative.3 - hematological profile.The hematological profile of our patient included PCV, WBC, neutrophil, lymphocyte, monocyte, count were measured using Emerald automated Hematology device. The results of these tests werenot compatible with the results of the blood culture, but normal result were compatible with negative blood cultures. The results adopted of blood culture confirm the presence of bacterial infection and then compared with theresults CRP test and blood profilefor the same samples.Negative result of blood culture and CRP test were 54 (33%) while positive results of blood culture and CRP test were recorded and improved after taking treatment for (, 3, 5, 7,) days for (40, 31, 5)patients respectively.ConclusionWeconcluded C - reactive protein test was useful in the diagnosis of bacterial blood infections and determine the stop - point otreatment. Gram negative bacterial isolates were sensitive impinim, livofloxacin and Amikacin while they were resistant to Ampicillin, Ampicillin /Sollbectam and Gentamycin. on the other hand gram positive bacteria were sensitive to Amikacin, Ampicillin/sulbactam Ciprphloxacin, and Tigecyclin and were resistant to Erythromicin, and Oxacillinand Benzylpencillin.

تحديد شدة الاصابة الحادة والمزمنة لداء المقوسات الكوندية بواسطة مستويات (IL - 6, IL - 8, TNF - ?) == Determination of Acute And Chronic Toxoplasmosis By Estimation of IL - 6, IL - 8 And TNF - ? Levels

Author name: لينا قاسم كاظم الزهيري
Supervisor name: بان نوري القاضي
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: المقوسات الكونديه من الطفيليات الشديده النجاح في انشاء اصابة مزمنه طويلة الامد. وقد اجريت الدراسة الحاليه على 366 امراة (117 مجهضات، 141 حوامل، 108 غير متزوجات) جمعت من مستشفيات مختلفة في بغداد. جمعت عينات الدم لجميع المجاميع المدروسة خلال الفترة مابين تش | Toxoplasma is a highly invasive parasite which establishes a life - long chronic infection. The present study was performed on 366 women (117 miscarriages, 141 pregnant and 108 singles) aged (17 - 35) years old from different hospital in Baghdad. Blood samples were collected from all patients during the period of Oct.2013 until of Jan. 2014. Anti - toxoplasma antibodies in the sera of all samples by using two tests, Latex test (LAT) and ELISA (IgG), (IgM) while, the proinflammatory cytokine were estimated by evaluation of the cytokine such as (IL - 6, IL - 8, TNF - ?). The results were showed that : ? The infection rate of toxoplasmosis by LAT was (41.53%) which was more sensitive than ELISA IgG (36.85%), and miscarried women were diagnosed by higher anti - toxoplasma IgG antibodies 62/117(52.99%), while pregnant result were lower 30/108 (27.78%). ? The most frequent age group of chronic infection with T.gondii was (23 - 28) year in both miscarriage and pregnant women, and represented by 45.16%, 40% respectively. While the most frequent age group for a single woman was (17 - 22) years, and represent 46.511% of the total number of this group.? Seroprevalence of toxoplasmosis by ELISA IgM test revealed low sensitivity in different studied groups 25/366(6.830%), and miscarried women were diagnosed by a highly significant (P ?0.01) percentage of acute toxoplasmosis14/117(11.965%).and The age group of (17 - 22) years old diagnosed high significant (p?0.01) percentage of acute toxoplasmosis in all studied groups.? According to the distribution of infected miscarried women with toxoplasmosis and gestational age by ELISA IgM the result showed that most of miscarriages were occurred at the first trimester which considered high significant (p? 0.01) 8/14(57.42%). While, chronic infected women at the second trimester (4 - 7 month) diagnosed by significantly (P?0.01) higher percentage of miscarried 24/62(38.71%).? The level of pro - infalmmatory cytokine (IL - 6) in acute infected miscarried women with toxoplasmosis distincted with a significantly high increase (P? 0.05) (177.31±12.53 Pg/ml) in comparison to chronic infected one (119.36 ±14.07 Pg/ml).While, chronic infected pregnant women was low significantly (P ?0.01) (48.41± 2.79 Pg/ml) in comparison to acute infected one and other studied groups, ? The acute infected singles was (118.98± 15.68 Pg/ml) higher non - significantly (P?0.05) than chronic infected one (112.65 ± 17.91 Pg/ml) and both disease activity were significantly higher than its level in healthy singles.? The level of TNF - ? in acute infected miscarried women distincted with significantly higher (P?0.05) (97.46 ± 5.37 Pg/ml) in comparison to chronic infected women (69.55 ± 4.58 Pg/ml), Whereas the mean level of TNF - ? in pregnant infected women during acute phase (62.31 ± 3.82 Pg/ml) was significantly (P?0.05) higher than chronic infection (21.42 ±2.74 Pg/ml)? Single infected women showed high significant increase (P?0.05) of TNF - ? level (50.48 ± 7.42 Pg/ml) in comparison to chronic infected one (35.46± 5.36 Pg/ml) and both disease activity scored high significant increase (P?0.05) of TNF - ? in comparison to healthy singles (18.29 ± 1.02 Pg/ml).? Finally, the mean level of IL - 8 in miscarried women with positive infection with T. gondii during acute phase (187.92 ± 15.38 Pg/ml) was significantly (P?0.05) higher than chronic phase of infection (140.57 ± 13.87 Pg/ml).Whereas the mean level of IL - 8 in acute infected pregnant women (110.32 ± 9.13 Pg/ml) was higher significantly (P?0.05) than healthy pregnant (91.49 ± 7.59 Pg/ml) and healthy singles (88.82 ± 5.62 Pg/ml).? Infected single women showed highly significant (P?0.01) increased levels in both diseases activity in comparison to single women without infection (88.82± 5.62 Pg/ml).

دراسة التاثيرات السمية للـ Gliotoxin المنتج من العفن Aspergillus fumigatus باستخدام تخمرات الحالة الصلبة == Study Toxicity Effects of Gliotoxin Produced By Aspergillus Fumigatus Using Solid State Fermentation

Author name: محمد عادل نوري
Supervisor name: عبد الكريم جاسم هاشم
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: تم اختبار قابلية عشرة عزلات للعفن Aspergillus fumigatus على انتاج الغليوتوكسين بوساطة تخمرات الحالة الصلبة وباستخدام الرز كوسط زرعي (ركيزة). اشارت نتائج الغربلة ان العزلة AF - 5 كانت هي الافضل واعطت اعلى انتاجية.كانت الظروف المثلى لانتاج السم على وسط الر | The ability of Ten Aspergillus fumigatus isolates for gliotoxin production were screened by solid state fermentation (SSF) using rice medium as substrate. The results indicated that, the AF - 5 isolate was the highest gliotoxin producer.The optimum conditions for gliotoxin production by AF - 5 isolate on rice medium were : - inoculum size 6×106 spores, moisturizing ratio (5 : 1) (w : v) with distilled water and incubation at 37 ?C for 10 days. Gliotoxin was purified from crude extract of AF - 5 isolate after production under the optimum conditions using solid state fermentation SSF. Purification of gliotoxin was achieved by two steps including : filtration and solid phase extraction. These processes were accessed to remove debris from crude extract and gives pure gliotoxin. The gliotoxin concentration was (122.6 ppm), which was detected by Thin layer Chromatography and High Performance Liquid Chromatography.The result of cytotoxicity effect of both purified extract and standard gliotoxin on human lymphocytes with exposure time of 24 hours at four different concentrations 25, 50, 100 and 200 ppb showed growth inhibition percentage 21, 39.10, 61.99, 87.45% and 17.89, 34.92, 58.34, and 85.22% respectively. The cytotoxicity effects have been done using conversion of the MTT to MTT formazan. After DNA was extracted from lymphocyte treated with toxin and analyzed by electrophoresis on a 1% agarose gel, gliotoxin appeared to have ability to damage the DNA. Results showed that both growth inhibition and DNA damage were increased gradually with the increasing of gliotoxin concentration.

تحديد بعض المعادن الثقيلة والتلوث البكتيري في الحليب الخام والمستورد المجفف == Determination of Some Heavy Metals And Bacterial Contamination of Raw And Imported Powder Milk

Author name: مروة صباح طالب
Supervisor name: محمد نافع علي العزاوي
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: يتعرض الحليب للعديد من الملوثات الفيزيائية والكيميائية والبيولوجية وذلك بسبب طرق الانتاج واسلوب النقل وعمليات التسويق ومن هذه الملوثات المعادن الثقيلة والرقم الهيدروجيني ودرجة الحرارة والبكتيريا. وقد صممت الدراسة الحالية لفحص عينات للحليب الخام التي جمعت | Milk is subjected to various physical, chemical and biological pollutants duo to producing, transporting and marketing processes such as certain heavy metals, pH, temperature and bacteria. The current study was designed to examine fresh milk samples were collected randomelly at early morning from six different locations at a rate of once each month during study period which commenced in October 2013 and ended in march 2014 and powder milk brands which were examined again for six months as in case of fresh milk where these milk brands were collected from local markets each month. The results of heavy metals showed that : • The investigation of Lead content in raw milk revealed that the highest mean value (1.801±0.311 ppm) was scored in Abo - Ghraib sample and the lowest mean value (0.941±0.104 ppm) was observed in Ghazaliya sample. For the Cadmium content in raw milk that the highest mean value (1.532±0.124 ppm) was found in Abo - Ghraib sample and the lowest mean value (0.063±0.044 ppm) was found in Fal'loga. The Copper content in raw milk also investigated and resulted the highest mean value was (0.931±0.092 ppm) in Azizia milk sample and almost similar value (0.931±0.301 ppm) was found in Madain sample while the lowest mean value was (0.308±0.029 ppm) in Fal'loiga sample. The highest mean value of Potassium in raw milk was recorded in two samples in Azizia (9718.8±246.6 ppm) and (9718.3±482.2 ppm) in Abo - Ghraib but the lowest mean value (4156.3±268.3 ppm) was recorded in Essaouira. Regarding Sodium content in raw milk, the results have found that the highest mean value was (2968.8±312.2 ppm) in Essaouira sample while the lowest mean value was (1432.5±156.8 ppm) in Abo - Ghraib. The Chloride content in raw milk samples, the highest mean value was (3053.0±128.6 ppm) recorded in Fal'loga sample and the lowest mean value was (639.0±198.4 ppm) was found in Azizia sample. Analysis of variance of these data showed significant effects (P?0.001) of both sampling sites and collecting months on milk (pb, Cd, K, Na and Cl) contents while analysis of variance of these data reveals no significant impact of sites on milk Cu content (P> 0.05) where no differences were observed between mean value of different collecting sites but shows highly significant (P? 0.001) effects of collecting months.• While powder milk has shown that the highest mean values of Lead (1.225±0.052 ppm) were found in Al - Mudhish brand and the lowest mean value (0.088±0.002 ppm) was detected in Anchor. The highest mean Cd value was (0.184±0.005 ppm) in Nido brand and the lowest mean value was (0.069±0.003 ppm) in Altunsa sample. While the highest mean value of Copper was (1.656±0.254 ppm) in Dielac powder brand and Anchor milk brand had the lowest copper mean value which was (0.767±0.014 ppm). The concentration of Potassium was (9625.0±462.9 ppm) which is the highest recorded in Dielac milk sample and the lowest mean value (3356.8±225.6 ppm) was found in Al - Marai milk sample. While the highest average of Sodium was (2625.0±342.6 ppm) scord in Dielac brand and the lowest average was (1745.0±338.4 ppm) recorded in Al - Marai sample. The highest mean value of Chloride (193.0±41.0 ppm) was recorded in Nido milk brand and the lowest mean value (122.7±22.2 ppm) was found in Altunsa milk sample. However, analysis of variance shows significant differences (P?0.001) between examined milk brands while no such differences were found between these brands (P>0.05) regarding collecting months.From the above results, it seems that the highest content of (Pb, Cd and Cu) are higher than those of standards of the International Dairy Federation (IDF) while contents of (K, Na and Cl) are within the standard requirement according to National Research Council (NRC).Microbial tests of raw milk samples were positive for bacterial growth, as different bacterial species were observed such as Lactobacilli spp. 63.8%, Streptococcus spp. 61.1%, Staphylococcus aureus 44.4%, Escheritia coli 88.8%, Bacillus spp. 72.2%, Salmonella typhi 72.2%, Staphylococcus epidermidis 30.5%, Pseudomonas spp. 47.2%, Micrococcus spp 27.7%, Clostiridia spp. 66.6%, Enterococci spp. 11.1% and K. pneumonia 75% from bacterial contamination of raw milk samples.However, only (11.1%) of the total collected samples of raw milk were suitable for human consumption and (88.9%) unsuitable, indicating that the source of infection or contamination of milk may be due to the animal health, human handler and the environmental factors, e.g., contaminated vessels, polluted water, flies and dust, etc… Obviously, bacterial contamination test of powder milk samples was carried out and negative results were obtained. However, it seems clearly that the opportunity of finding bacterial contamination in powder milk samples may be very rare since milk powder is produced in well designed and modern techniques. Nevertheless, a single bacterial contamination test has indicated to a positive growth of Staphylococcus aureus colonies.

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L == The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

Author name: مروة قيس ابراهيم العبيدي
Supervisor name: ماهر زكي فيصل الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

التحري عن جين الاوتولايسين في المكورات العنقوديه البشروية المقاومة لمضاد الفانكومايسين == Autolysin Gene Detection In Vancomycin Resistant Staphylococcus Epidermidis

Author name: مريم خميس عبد ربة بريس
Supervisor name: مي طالب فليح
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: من مجموع مئة عينة سريرية جمعت من مصادر مختلفة شملت الحروق والدم والجروح ومسحات انفية, تمكنت 90 عزلة من النمو على اكار المانيتول الملحي , ومن بينهم 40(44.4 %) عزلة عائدة لبكتريا المكورات العنقودية المنتجة للانزيم المخثر للبلازما و50 (55.5%) عائدة لبكتريا ا | Out of one hundred clinical samples were taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci in which Staphylococcus epidermidis isolates were 30(60%). The pattern of antibiotic susceptibility of Staphylococcus epidermidis isolates to 12 antibotics (Amoxiclav, Ceftazidim, ciprofloxacin, clindamycin, Erythromycin, Gentamycin, Imipenime, Penicillin G, Tetracycline, Rifampin , Methicillin, and Vancomycin) were determined using disc diffusion method. The results revealed that resistance to Penicillin G10 and Amoxiclav (Amoxicillin - clavulanic acid) were 100%, Methicillin were 93%, Erythromycin were 90%, Gentamycin and Clindamycin were 70%, Tetracycline and ceftazidim were 75%, Ciproflaxacin were 60%, Rifampin were30%. 95% of S.epidermidis isolates were sensitive to Imipenim and 5% of them were intermediate resistant, while these isolates showed 90% sensitivity to vancomycin. 19 isolates were multidrug resistance. Minimum inhibitory concentration of S.epidermidis isolates to vancomycin, was determined. The results revealed that (12) S.epidermidis isolates (40 %) were vancomycin resistant, the MIC of them were between 256 ?g\ml and 32 ?g\ml, (4) S.epidermidis isolates (13.3%) were intermediate resistance, the MIC to 3 of them were 16 ?g\ml and the last was 8 ?g\ml. Some virulence factors of VRSE and VSSE were detected including the hemolysin, protease, lipase and urease. The S. epidermidis isolates were produce hemolysin, protases, lipase and urease, were 100%, 100%, 25%, 100% respectively in VRSE, while in VSSE 100%, 100%, 60%, 100% respectively. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA, vanB and autolysine gene aae gene. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene. All S.epidermidis were able to produce implicone size(858bp) of aae gene. The effect of vancomycin resistant S. epidermidis on cell autolysis activity was detected by whole cell autolytic assay.The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis 1) have thicker cell wall followed by VISE (S.epidermidis 14) isolates.However, the VSSE (S.epidermidis 22) didn't showed any cell wall thickening.

تاثير المستخلص الكحولي لنبات الخباز (Malva sylvestris ) في فسلجة ونسجية الكبد والخصية في ذكور الفئران البيض == Effect of Alcoholic Extract of Mallow (Malva Sylvestris) On Physiology And Histology Liver And Testis In Albino Male Mice

Author name: بسمة علي جاسم
Supervisor name: مختار خميس محمد سعيد
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: صممت هذه الدراسة للتعرف على مدى تاثير المستخلص الكحولي الخام لنبات الخباز Malva Sylvestris في الكبد والخصى لذكور الفئران البيض نسجيا, وكذلك فسلجيامن خلال دراسة مستوى انزيمي Glutamate - oxaloactate - transaminase (GOT) وGlutamate - pyruvate - transaminase( | This study was designed to investigate the effects of crude alcoholic extract of Malva sylvestris on the histology of liver and testes in male albino mice, as well as levels of the GOT, GPT enzymes and testosterone hormone. Seventy two adult male albino mice were used in this study. The animals were randomly divided into four main groups, the first group was given distilled water (control), while the other three groups were given (100, 300, 500 mg /kg) of crude extract. These main groups were subdivided into three subgroups, according to the period of treatment (2, 4, 6, 8)weeks. Blood samples were collected every two weeks for physiological study, and samples of the liver and testes were collected for histological study. Treating with crude alcoholic of Malva sylvestris led to no significant increase in the mean of the body weight except the concentration (500 mg /kg). There was significantly increase in the liver weight particularly in(500 mg /kg) for eight weeks of exposure, also there was significantly increase in the average of the testes weights treated with Malva sylvestris extract specially in (300, 500 mg /kg) for all periods of treatment compared with control group. Malva sylvestris extract coused significantly decrease in both GOT, GPT. there fore the value of GOT, GPT considers as concentration - period exposure dependent the highest value of GPT was (300 mg /kg) for two weeks (2. 25±228. 04) IU/L compared with control group (3. 03±226. 02) IU/L. and the lowest value of GPT was (500 mg /kg) for eight weeks (4. 52±192. 27)IU/L compared with control group (4. 06±208. 77) IU/L the highest value of GOT in (100 mg /kg) reached (4. 10±63. 00) IU/L for two weeks compared with control group (1. 25±71. 23) IU/L. the lowest value was (2. 77±49. 83) IU/L in (500 mg /kg) with eight weeks comparison with control group (1. 18±69. 02) IU/L. all concentration of Malva sylvestris extract made positive changes in the value of testosterone with all periods of exposure particularly in (300 mg /kg) compared with control group. There were many histopathological alterations appeared in the liver tissue among treated groups which caused by the effects of Malva sylvestris extract represented by congestion, disarrangement of hepatic cords, beginning of necrosis of hepatocytes, infiltration of leukocytes, narrowing of sinusoids, increase the number of binuclei cells, degeneration, appearance of giant cells, all these changes were reversible. Malva sylvestris extract made beneficial histopathological changes in the testes tissue in all treated groups like increase in numbers of ledyig cells specially concentration (500 mg /kg) reached (11.37± 0.80) IU/L with two weeks comparison with control group(5.38 ±0.34) IU/L, and increase in the thickness of the walls of seminiferous tubules specially concentration (500 mg /kg) reached(29.46± 1.39) IU/L with compared with control group(20.52±2.40) IU/L. In addition the role of extract in significant decline in the diameters of seminiferous tubules in all treated groups compared with control group

نوعية البويضة والتكوين الجنيني بعد الاعطاء الفموي للسبيرماكس لاناث الفئران : موديل تجريبي للبائن == Oocyte Quality And Embryonic Development After Oral Administration of Speramax® In Female Mice : Experimental Model For Mammal

Author name: هبة صاحب حمزة
Supervisor name: سعد صالح الدجيلي
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: Background : Speramax® has been found to play an important role in sperm function characters and males reproductive performance with no studies on its effects on the oocyte maturation and embryonic development in females.Objective : The goal of the thesis is to examine the effect of Speramax ® on oocyte maturation. And to emphasize the effect of Speramax ® on ova quality, embryonic development and newborn. Materials and Methods : Speramax ® treated by oral administration for 1, 2 and 4 weeks. A hundred and twenty six female mice were randomly divided into four groups, the first group was treated without Speramax® with superovulation(SUO) while the second group were treated by Speramax® with SUO and the third group were superovulated only and the fourth group was not treated and spontaneously ovulated (SPO) and considered the control group(thirty two mice). Results : The results indicated that the treatment with Speramax® showed a positive effect on oocytes maturation in vivo. There was a highly significance (p? 0.0001) improvement in number of mature oocytes following treatment with Speramax® in SPO and SUO mice compared with SPO and SUO mice not treated with Speramax®. The embryonic developmental rate after 24 and 48 hours of mating in treated groups with Speramax® was significantly (p? 0.05) higher than those of SPO and SUO mice too.The study showed that the quantity and quality of embryos generated from the treated groups were superior to that of untreated groups.Conclusion : It was concluded that the treatment by Speramax® has a great improvement on oocyte maturation, early embryonic development and embryo grading quality of mice embryos with an increase in the numbers of mice newborn.

الفعالية المضادة للاحياء المجهرية والانزيمية للفطريات المستنبتة المعزولة من نبات الاس Myrtle (MyrtuscommunisL.) == Antimicrobial And Enzymaticactivity of Endophyticfungiisolatedfrommyrtle (Myrtuscommunisl.)

Author name: هدى محمد كاظم ديوان
Supervisor name: بتول زينل علي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: استهدفت الدراسة الحالية عزل وتشخيص الفطريات المستنبتة في اوراق نبات الاس Myrtus communis واختبار فعاليتها المضادة للاحياء المجهرية وفعاليتها الانزيمية. - اظهرت نتائج عزل الفطريات المستنبتة في 500 قطعة اوراق للحصول على عدد كلي للعزلات مساويا الى 99 عزلة ف | The present study was aimed to isolate and identify endophytic fungi from leaves of myrtle Myrtus communisand study their antimicrobial and enzymatic activities. - Results of fungal isolation from 500 leaves fragments revealed isolation of 99 endophytic fungi represented a total colonization frequency (CF) of 20.4%. These fungi were related to 23 species or isolates, where Aspergillus spp. prevailed over all others (11 species) with CF of 14% for all Aspergilli, Aspergillusniger and Aspergillusflavus showed the highest CF 7.2% and 3% respectively, other Aspergilli represented Aspergillusparvulus (CF 1.6%), Aspergilluscandidus (0.4%), Aspergillusraperi, Aspergillusclavatus, Aspergillusglaucus, Aspergillusornatus, Aspergillussclerotioniger, Aspergillusterreus and Aspergilluswentii were all showed CF of 0.2%.Other isolates fungi from leaves fragments were four morphologically different isolates of Penicillium with 0.4% CF for each. Additionaly, isolates of Cladosporium sp. (2%) and Cladosporiumcladosporoides (0.4%), as well as Cunninghamella sp., Drechsleraaustraliensis, Alternariaraphani, which showed 0.2% CF for each, Paecilomyces variotii (0.6|%), and different isolates of sterile fungi (White and Hyaline colonies) were also obtained. - Study the antagonistic activity of isolated endophytes using dual culture method against five plant pathogenic fungi(Fusarium oxysporum, Fusarium graminearum, Fusarium sp., Macrophomina phaseolinaandRhizoctonia solani)taking into account the percentage of inhibition of pathogenic fungi, results revealed variation of antagonistic activities between endophytes and pathogens. Different cases of antagonism were also appeared ranged from mycoparasitism of the endophytes on pathogens or vice versa, to competition between the two fungi and to the appearance of inhibition zone between them, this result may indicates the affinity of using some isolated endophytes in biological control applications against or controling fungal plant diseases. - Effect of ethylacetate extracts of culture broth of active endophytes shown in the last experiment against two bactria E.coli, S.aureus and the yeast Candida albicans using disc diffusion method, where filter paper discs were imprignated with different concentrations of fungal broth extracts. Results showed variation of inhibitory activity against bacteria with different endophytes, bacteria and conc. of the extract. Regarding the effect onC.albicans and filamentous fungi, most fungal broth extracts showed no inhibitory activity against yeast and no extract as well showed inhibitory activity on filamentous fungi. These results give indication thatbroth extracts contain antibacterial compounds. - Addition of 20% crude fungal broth of some endophytes to the culture medium showed significant inhibitory activity against filamentous fungi which did not respond to broth extracts, percentage of inhibition ranged between 0 - 77.08% and maximum inhibitory activity was shown by Penicillium sp. Isolate. These results give indication that some of crude filtrates contain antifungal compounds. - Analysis of broth extracts of some endophytes using TLC technique showed the content of extract of number of UV illuminated spots isolated on TLC pates having different Rf values, Bioautography showed inhibitory activity of some of these isolated spots against E.coli, S.aureus and the Filamentous fungus F.oxysporum. - Testing the extracellular enzymes activities of isolated endophyles on solid media, enzymes included amylase, lipase, pectinase, laccase, cellulase and protease showed variation in secretion of these enzymes, as well as, no endophytes showed the affinity of secreting all tested enzymes. Results also showed the high affinity of some endpohytes to secrete enzymes that could be use in multiple important applications after separation andcharacterizationof these enzymes.

التحري عن نوعية مياه الشرب لمحطتي معالجة مياه الحي والبشائر في محافظة واسط جنوبي العراق == Investigation of Drinking Water Quality In Al - Haay And Al - Bashaar Water Treatment Plants In Wasit Province Southern Iraq

Author name: وسام باسم محمد التميمي
Supervisor name: احمد جاسم محمد العزاوي
General topic: Biology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: م اجراء فحوصات فيزيائية وكيميائية واحيائية لمياه الشرب في محطتي الحي والبشائر وعدد من المناطق التي تغذيها, شهريا للمدة من تشرين الاول 2013 الى شهر تموز 2014, عبر مراحل التصفية وصولا الى المنازل التي تقع على مسافات مختلفة عن مصدر التجهيز وبواقع نموذجين لكل | Physical, chemical and a biological tests were carried out and drinking water samples were collected from AL - Haay and AL - Bashaer water treatment plants and number of residential areas fed by these plants for period extended from October 2013 to July 2014, through the purification stages up to residential sites at different areas situated with various distances from the supplying source, two samples were taken monthly. Air temperature at sampling time was varied from 16°C to 42°C and from 10°C to 34°C, for water temperature at sampling time also. The results of pH values were within the allowable limits, ranging from 7.1in autumn to 8.1 in summer. For EC, the highest value recorded for raw water was in winter with 1338 µs/cm, while the lowest value was in spring with 920 µs/cm. The highest value recorded for drinking water was again in winter with 1330 µs/cm, and the lowest value was 910µs/cm in spring also. The highest value of turbidity recorded for raw water was in winter with 89 NTU and the lowest value was in spring with 26 NTU, while the highest mean value recorded for drinking water was 20 NTU again in winter and the lowest value was 1 NTU in spring. The study showed that the results of TDS values ware ranged from 622 mg/L in spring to 1024mg/L in winter. The highest value of residual chlorine was recorded in summer with 4.5 mg/L; the lowest value was zero mg/L in some of the farthest points of the plants. For sulfates, values were ranged from 289 mg/L in spring to 498.4 mg/L in winter. All the recorded values of chlorides ions for all water samples were within the allowable limits, its values ranged from 98mg/L in summer to156.3 mg/L in winter. For total hardness, the values were exceeding 500mg/L and allowable limits for Iraqi standard criteria. The highest value recorded for raw water was in winter with 520 mg/L and the lowest value was in summer with 351 mg/L, while the highest value recorded for drinking water was again in winter with 516mg/L and the lowest value was 337 mg/L in summer also. For calcium values ranged from 75 mg/L in summer to 135 mg/L in winter. The results of this study showed high levels of lead, cadmium and aluminum in most water samples which collected during study period compared with those that exceeded the acceptable limits provided from the quality control unit and which are considered to have adverse effects on health. For lead, values were found to vary from 0.0030 mg/l in summer to 0.16 mg/l in winter (higher acceptable limits is 0.01 mg/L). While in case of cadmium the values lied between 0.0014 mg/l in winter and 0.015 mg/l in summer (higher acceptable range is 0.0030). However, for aluminum these data were ranged from 0.0062 mg/l in raw water during spring to 0.29 mg/l in drinking water during spring also (higher acceptable range is 0.2). The current results showed an increased in the number of autotrophic bacteria, total coliform, fecal coliform, fecal streptococci, and E. coli during winter season in most study locations as compared to those of the other seasons for both water plants raw and drinking water while the FS values were less than those of other bacterial types for both water plants. The Total plat count results of drinking water were exceed 100 cell/ml, the allowable limit for drinking water, for some samples of both water plants. on the other hand, the TC, FC and E. coli exceeded zero cell/100ml, the allowable limits for drinking water, in many drinking water samples for both water plants

دراسة بعض تاثيرات اللقاح المحضر من العزلة المحلية لبكتيريا Klebsiella pneumoniae == Study of Some Effects of Prepared Vaccine From Local Strain of The Klebsiella Pneumoniae

Author name: ياسر عبد الجبار عبود السوداني
Supervisor name: عصام فاضل علوان الجمیلي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: تم جمع خمسين عينة سريرية من قشع مرضى مصابين بذات الرئـــة. وذلك للمدة من تشرين الثاني 2013 ولغاية ايار 2013 من مستشفى ابن البلدي ومستشفى بغداد وذلك لعزل وتشخيص بكتريا Klebsiella pneumoniae التي تعد احدى العوامل المهمة المسببة لاصابات الرئة. واخضعت عينات | Fifty clinical samples collected from sputum of patients who suffered from pneumoniae in Ibn - Balady hospital and the hospital in Baghdad city during the period from November 2012 to May 2013 for the isolation and identification of Klebsiella pneumoniae, one of the important causative agents of infection occurs in the lungs. Sputum samples were subjects to the standard laboratory procedures including identification by biochemical test and VIETK system. The results showed 15 isolates were revealed as Klebsiella Spp, only 10 isolates represented K.pneumoniae, The isolates were examined to produce extracellular toxic complex (ETC) it was found that the isolate named K2 was the higher production. Two method for purification the extracellular toxic complex (ETC) were used, first Aqueous two phase systems, In this method polymer - salt aqueous two phase system was evaluated in crude extract of K. pneumoniae at varying concentration of Dextran T - 150 with 20% with polyvinyl pyrrolidone to final rate (1 : 1) (wt : wt) with 0.2M sodium sulphate. The results showed the best concentration dilution sample given as (4.25 : 0.75) with protein concentration (97.173 mg/ml) which contained ETC in the lower layer and the mice died within 4 hours, while the second method performed by using two step column chromatography, ion exchange DEAE - Cellulose and gel filtration (Sepharose - 4B). In the first step sample given lethal activity by injection to the mice after six hours with protein concentration (55mg/ml), More purification by the second step animal died after 3hours with contain protein (27.75mg/ml). Furthermore, the results of the extracellular toxic complex characterization proved that molecular weight was 39810 Dalton determined through Gel - filtration chromatography using Sepharose 6B gel. The LD50 value of purified toxin was calculated, and the result was (6.52 mg/ml) of toxin.This quantity was found effective to cause killing of 50% of the total toxin treated animals. The biological effect of purified toxin of K. pneumoniae K2 have been examined in vivo by injection of dose (0.5 ml) of purified ETC toxin that contain (10.875 mg/ml ) protein. The final part of the study involved the histopathological changes were noted, abundant mononuclear infiltrate of inflammatory cells with necrosis of lung parenchyma. The second group of mice injected with (0.05 ml of ETC) that contain protein (1.085mg/ml) represented as sub lethal dose Histopathological changes were noted showing near of the normal appearance of alveoli and alveolar space, with presence of congestion of blood vessels. The third group of mice inject with (0.5 ml from Tris - base buffer only) represented control showed normal alveoli and alveolar space with presence of bronchial. In the immunological test the sample ETC examined with ELISA and given IgG titer (189.68+50.70 ng/ml) compared with control (46.78+12.45). This titer of IgG tested with Double immune diffusion assay and gave precipitation line with antigen compared with control.

الكشف عن الرز المحور وراثيا باستخدام انواع مختلفة من التفاعل الانزيمي المتسلسل PCR == Detection of Genetically Modified Rice By Different Type of PCR

Author name: ياسمين ابراهيم فرحان
Supervisor name: امنة نعمة الثويني
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: In recent years, foods produced by genetic engineering technology have been on the world food markets. The biosafety aspects, regulations, and labeling for these foods are still contentious issues in most countries. Thus detection and quantificationof GMOs play crucial role for developing regulations on GM foods.In this study, eighty six non - labeled rice samples from different locals and exported market were analyzed to detect the genetic modification using a DNA based detectionvmethods as, conventional Polymerase chain reaction (PCR), and Real time PCR (RTPCR).The DNA rice samples were extracted by manual C - hexadecyl - Trimethyl - Ammonium - Bromide (CTAB) method and wizard kit method. The result revealed that DNA yield by the two methods is comparable. Rice DNA tends to be of a higher concentration when purified with the CTAB method; however, this particular DNA is more easily to amplify, the optical density (OD) was recorded 1.70 - 1.98 and the concentration of DNA quantified by fluorometer DNA rice samples, ranged from 11 to 50.5 ?g/?l. The DNA rice sample has also been used successfully with the Wizard Genomic DNA Purification Kit, and showed varieties in quality, the OD was recorded 1.65 - 1.95, and the concentration between 4.7 - 43.8 ?g/?l.The rice specific gene (sps gene) was detected by PCR. The results demonstrate that the purity of the extracted DNA in all tested rice samples was sufficiently high for a sensitive PCR analysis and the primer of detected gene appeared clearly at 251pb.Three genes; CaMV 35S promoter, NOS terminator, and insecticide resistant gene Cry1Ac were used to detect of GM rice by PCR, and Real time PCR using oligonucleotide sets targeting to novel genes. The result showed that there was no positive result reaction with conventional PCR, while the outcome of gradient PCR revealed a positive reaction in one sample (Uncle Bens brown) for CaMV35S promoter only. Gradient PCR with 12 replicons for each sample was used for qualitative detection of CaMV35S promoter gene, after optimization of melting temperature and cycles run (45 cycles) , the results appeared positive in the last three grades (63.9, 64.6, 64.9) for CaMV35S promoter, but NOS terminator, and CryIAc were recorded negative results.The result of Real - Time PCR clarified that the CaMV35S promoter specific primer showed strong amplification with Ct, and Tm values were reached into 33.73, 38.63 and 61.55, 62.92 in two samples Uncle Bens brown and Himalayan brown, respectively, whereas NOS terminator gave positive results in four samples Maxims, Laasturiana, Carolin white and Mahatma, and the values Ct and Tm reached to30.87, 30.31, 30.54, 33.75 and 64.53, 64.61, 62.62, 63.87 respectively in comparison with the positive control, while CryI Ac which did not show any positive signal.It was concluded that using molecular methods like Real - time PCR will be useful tool for detecting GM rice such as a part of the approval detection processes because of the rarity of data concerning consumption of GM rice in Iraq.

تاثير الاصابة بداء المقوسات الكوندية على المستويات الهورمونية والمدورات الخلوية خلال فترة بلوغ الانسان في محافظة بغداد == The Effect of Toxoplasmosis On Hormonal And Cytokines Levels During Human Maturity In Baghdad Province

Author name: ياسمين رياض عبد الكريم الخناق
Supervisor name: صباح ناصر العلوجي | خولة حوري زغير
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: داء المقوسات الكونديه مرض عالمي الانتشار حيث يصاب معظم الاشخاص ذوي المناعة الجيدة بطفيلي المقوسات الكونديه وغالبا دون ظهور اعراض. ان الهدف الرئيسي لهذه الدراسه هو فهم الفروقات الجنسيه, الهرمونيه والمناعيه في سن البلوغ في الاشخاص الذين لديهم اجسام مضاده نو | Toxoplasmosis is a worldwide disease where most healthy, immunocompetent individuals infected by Toxoplasma are almost asymptomatic.The primary goal of this study is to perceive the hormonal and immunological sex - differences in puberty age who have positive anti - Toxoplasma IgG specific antibodies. The secondary goal is to inspect the endocrine - immune interaction in these persons by detecting the effect of testosterone and oestradiol hormones level on cellular immune response namely, IL - 4, IL - 12. From the first of November 2012 till the end of April 2013, 303 blood samples were collected from apparently healthy male and female students of Al - Erfan, Ignadeen and Algawahery schools and Baghdad University, Both sexes where divided into two age groups : group (A) which included subjects with age range (12 - 15) years old and group (B) which included subjects with age range (16 - 19) years old. All serum samples were tested for toxoplasmosis by using Latex agglutination test and ELISA anti - Toxoplasma IgG antibodies test. As well as, all serum samples were tested by using ELISA technique for detection of serum mean concentration of testosterone, oestradiol hormones, IL - 12 and IL - 4. The results revealed that 107/ 303 (35.31%) of the studied subjects showed seropositive toxoplasmosis, 60 males and 47 females of 107 positive samples showed high significant (p<0.01) differences in comparison to uninfected subjects. Males group B have recorded the highest percentage 34(41.46%) of the infection. Positive association was found between toxoplasmosis and testosterone level in asymptomatic toxoplasmosis cases compared to uninfected group. high mean concentration of testosterone in toxoplasmosis infected males recorded (15.03± 1.04 ng/ml) and (12.4± 0.91 ng/ml) in groups A and B respectively, in comparison to control group which recorded (8.03± 0.78) and (9.86± 0.83) in groups A and B respectively. Also toxoplasmosis infected females revealed high levels of testosterone hormone which represented (4.83± 0.06 ng/ml) and (2.55± 0.03 ng/ml) in groups A and B respectively, with a significant (p?0.05) differences between them, while the control group recorded (0.10± 0.02 ng/ml) and (0.90±0.03 ng/ml) in group A and B respectively.The present study showed a significant (P?0.05) decrease in the mean concentration of oestradiol E2 hormone in toxoplasmosis infected males and females in comparison with uninfected ones. E2 mean concentration was (41± 2.48 ng/ml) and (56± 2.91 ng/ml) for male groups A and B, respectively, in comparison to control group which recorded (67± 2.08 ng/ml) and (74± 2.42 ng/ml) in group A and B respectively, while it was (188 ± 12.48 ng/ml) and (196 ± 16.52 ng/ml) for female group A and B respectively, in comparison to control group which recorded (221±12.09 ng/ml) and (233± 15.63 ng/ml) for group A and B, respectively.The mean concentration of E2 hormone in toxoplasmosis infected females according to their menstrual cycle showed low levels in ovulation, late follicular and luteal phases, which represent (37.5 ± 2.59 ng/ml), (131 ± 16.7 ng/ml) and (76± 3.92 ng/ml) respectively, while the mean concentration of this hormone in uninfected females was (52.4± 2.88 ng/ml), (271.6± 21.04 ng/ml) and (196.2± 12.37 ng/ml) in the three phases of menstrual cycle. This study showed high significant (p?0.05) level of IL - 12 in both males and females with latent toxoplasmosis in comparison with free - toxoplasmosis groups. The mean concentration of IL - 12 in infected males was (4.75 ± 0.88 pg/ml) and (4.12 ± 0.69 pg/ml) in male groups A and B respectively, in comparison to control group which was (2.86± 0.53 pg/ml) and (2.46± 0.62 pg/ml) in groups A and B respectively, while it was (5.60 ± 0.12 pg/ml) and (6.04 ± 0.26 pg/ml) in infected female groups A and B respectively, in comparison to control group which recorded (3.32± 0.89 pg/ml) and (4.27± 0.15 pg/ml) in group A and B respectively. IL - 4 recorded quite elevated level in toxoplasmosis infected males (groups A and B) which was (15.09 ± 0.92 pg/ml) and (17.67 ± 0.78 pg/ml) respectively, in comparison to control group which recorded (13.89± 0.84 pg/ml) and (14.92± 0.69 pg/ml) in groups A and B respectively, Meanwhile the mean concentration of IL - 4 in toxoplasmosis infected females showed mild elevation in both groups A and B which was (15.14 ± 0.84 pg/ml) and (16.06 ± 1.13 pg/ml) respectively, in comparison with toxoplasmosis free subjects which recorded (16.53± 1.22 pg/ml) and (15.15 ± 0.97 pg/ml) with no significant differences between them. Interactions between the endocrine and immune systems may mediate sex differences in response to toxoplasmosis infection.

التحري عن التعبير الجيني لل FOXP3 وTGF - ?1 باستخدام الطرائق الجزيئية والمناعية في سرطان الرئة اللاصغير الخلية == Detection of FOXP3 Gene Expression And TGF - ?1 Using Molecular And Immunological Methods In Non - Small Cell Lung Carcinoma

Author name: سهاد فيصل حاتم المقدادي
Supervisor name: امنة نصيف جاسم | بان عباس عبد المجيد
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
Abstract: Studies have linked FOXP3 and TGF - ? expression to the outcome of certain cancers. FOXP3 is a marker known to be expression in T - regulatory cells while TGF - ? is a secreted protein usually detected in the extra cellular matrix.The present study aimed at focusing on the identification of immune markers namely FOXP3 and TGF - ? with their expression patterns in lung cancer patients as a useful tool to predict disease progression.Also it is aimed to design molecular evaluation of m RNA expression of both FOXP3 and TGF - ? in peripheral blood mononuclear cells and bronchial (brush) cells of patients with lung cancer and benign lesions, using qRT PCR; determining the T - reg level in the peripheral blood employing the High Rsolution Melting (HRM) as a novel method to detect Treg - specific demethylated region (TSDR); molecular DNA analysis of somatic mutation of exons 3, 6, 7 of FOXP3 in patients with lung cancer tissue and benign lesions and immunohistochemical (IHC) estimation of FOXP3 and TGF - ?1 in T - reg and cancer cells in formalin fixed paraffin embedded(FFPE)lung cancer tissue and benign lesions.Blood samples were collected from 30 patients with newly diagnosed, non small cell lung carcinoma and 30 patients with benign lesions.Patients were recruited at The Specialized Surgery Hospital and Oncology Teaching Hospital/Baghdad.Samples from 16 apparently healthy donors were used as control during the period from June 2012 to June 2013. The samples preservation with TRIzol reagent were subjected to molecular study including RNA and DNA extraction; reverse transcription; RT - PCR; HRM assay and DNA sequencing were done in the Molecular Oncology Unit/Guys and ST Thomas? s hospital/ King College/London/UK.The expression level of FOXP3 was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of FOXP3 expression(fold change)was significantly high(2.64±0.09)in cancer cases than in benign cases(1.32±0.04)and healthy control(1.38±0.06)with p<0.05.A significant association between high expression level and >60 age and squamous cell carcinoma in cancer cases P<0.05.The expression level of TGF - ? was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of TGF - ? expression (fold change) was significantly high (6.27±0.56) in cancer cases more than healthy control (2.87±0.09) with p<0.05.The association was significant between TGF - ? expression level (high and low) and age>60in cancer and benign groups (p<0.05), while no significant association with gender and cancer types were noticed.For FOXP3 mRNA expression in bronchial brush cells, the result showed no significant difference between the mean fold change of malignant(3.57 ± 0.06) and benign(4.02 ± 0.06) patients. The low expression was predominated both in cancer and benign cases. No significant differences were found between FOXP3 expression (high and low) and age; gender ; cancer types..According to FOXP3 T - reg specific demethylated region detection, results showed that the mean percentage of FOXP3demethylation in lung cancer patients (4.32 ± 0.04) was significantly higher than in benign lesions (3.22 ± 0.02) patients andhealthy controls(3.33±0.04). A positive correlation coefficient with high significant, was found in the group of cancer samples (R² = 0.6653;r = 0.69;P : 0.0017)on correlating percentage of Treg and demethylation of FOXP3 from one side with its m RNA expression on the other side.In benign lesion group was(R² = 0.5334;r= 0.59;p= 0.0027), While in the control group a positive correlation but a weak significance was found (R²= 0.2383;r=0.28;P=0.0437).FOXP3 gene sequencing revealed high frequency of missense mutations c.715 GTA>CTA : V 239 L in 17 (94.44%) in malignant sample and non cancerous cases7 (87.5%)without statistical differences. Missence mutations were also detected in exone 3 in 3(16.67%) cancer cases and in 1(12.5 %) benign lesion.No missense mutations could be detected in exon 6. Intronic mutations and silent mutation were variable in three exons without statistical differences. Many cases of adenocarcinoma have shown multiple mutations either of missense or Intronic types. Missense mutations of exon 7 were correlated significantly with an age of 60?years. Exon 3 mutations were significantly associated with adenocarcinoma. Positive FOXP3 Immunohistochemistry (IHC) staining in tumor cells was associated with high missense mutations frequency 10(55.55%) in exon7, while in exone 3 was 2(11.11%). Negative FOXP3 IHC staining in the tumor cells was associated with seven missense mutations in exon7 and one (5.55%) in exon 3, in addition 4(23.53%) cases of the exon 7 missense mutations were associated with negative FOXP3 expression in lymphocytes.The result showed that FOXP3 by using IHC staining was positive in 21(70%) of nuclei of cancer cells, and 22(73.3%)in Treg infiltrates.The positive cancer cells and Treg infiltrates associated significantly with age>60 (p<0.05).No significant association was found withgender, cancer type, while there is association with moderate differentiation compared to poorly differentiation (p<0.05). High frequence of FOXP3 expression score 3 and high intensity were appeared in nuclear cancer cells compared to benign lesions cells, while Treg infiltrates with score 1 and high intensity was high frequency in malignant and benign.The result showed that TGF - ?1 by using IHC staining was positive in 25(83.3%) in the cancer cells and 21(70%) in stromal cells. No significant difference was noted between positive expression in malignant and benign lesions p>0.05.No significant association was noticed between positive cells expression and age, gender, cancer type and differentiation p>0.05. High frequency of TGF - ?1 expression score 3 and high intensity in malignant cells and benign. Also stromal cell expression score 3 and high intensity were predominated in malignant and benign lesions. The high and moderate intensity expression was more frequent in matrix surrounding cancer cells compared to non cancerous.Total agreement and kappa coefficient between FOXP3 and TGF - ?1were poor in malignant and benign epithelial cells and stroma, while the perfect agreement was between expression of TGF - ?1in stromal cells
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