Show: 25 50 75 100 Results

Search results: 5 out of 5

ارتباط بعض اليلات الصنف الثاني HLA في الحساسيه للحم الاغنام والماعز في محافظة البصرة == Association of some of HLA class II alleles with allergy to lamb and goat meat in Basrah province

Author name: رواء بناي زبيري
Supervisor name: فوزية علي عبد الله | عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Biotechnology
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine
Language: English
University location: Basrah
First pages:

الكشف السيرولوجي والجزيئي لفايروس مرض الحمى القلاعية في الابقار في محافظة البصرة == Serological And Molecular Detection of Fmdv In Cow of Basrah Province

Author name: زينب مجيد سالم الكلي
Supervisor name: عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Biotechnology
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: English
University location: Basrah
First pages:
Abstract: اجريت هذه الدراسة في6 مناطق مختلفة ( شط العرب والزبير والقرنه وابي الخصيب ومركز البصره والمدينه) ممثله لجميع اجزاء محافظة البصره خلال الفتره من شهر تشرين الاول من العام 2012 الى شهر تموز من عام 2013 ولتحديد نسبة انتشار مرض الحمى القلاعيه في الابقار استخد | This study was conducted on local cattle from 6 different regions (Shutt - Alarab,Al - Zubair, Al - Qurna, Abi - Elkhasib, Basrah center and Almdaina) covering all parts of the Basrah province during the period from October 2012 to July 2013. In order to determine the prevalence of foot and mouth disease in bovine species Enzyme Linked Immune Sorbent Assay ( ELISA) and reverse transcriptase polymerase chain reaction techniques( RT - PCR) were used in the testing of serum ,saliva and vesicular fluid samples collected from clinically diseased cattle, moreover this study was also mentioned some clinical aspects of the disease.Diseased animals showed the following clinical signs : depression(92.1%),sever salivation(90.9%) ,vesicles in mouth(90.3%), fever(89.1%) , conjunctivitis(87.9%) and smacking of lips in higher rates( 80.6%).While the vesicles on teat(12.7%), lameness(36.9%)and vesicles on feet (45.5%)appeared in lower rates.The overall prevalence of this disease was(72.7%.).Of 165 serum samples subjected to nonstructural protein (NSP) ELISA screening test (ELISA CHEKIT FMD - 3ABC Bo - Ov ELISA Test ) , 120 were interpreted as positive representing (72.7%.) while the other 45samples were negative representing 27.3%.The seropositivity significantly associated with age groups (p<0.01) and sex (P < 0.01).Depending on sex of these animals , the higher seropositivity rate was observed in females (78.6%).Concerning the age of tested cattle the animals of 2nd age group (>4 - 8 years) appeared in higher rate (84.8 %) of seropositivity followed by the rate (51.7% ) of 1st age group animals(<1 - 4 years).The sero - positivity against FMDV was non significantly different(>P 0.05)among the cattle in Basrah districts and highest sero - prevalence rate was observed in cattle of Basrah center (100%), Al - Mdaina (77.8%), Shut - Alarab(76.3%) and Abi - Elkhasib (70%).The lower sero - positivity rate was observed in Qurna(58.8%)and Zubair (50%) cattle.RT - PCR detection of FMDV for primary and serotype specific diagnosis was used. of eighty three clinically positive samples including, mouth vesicles, saliva and serum samples tested by RT - PCR, only 68(81.9%) were successfully amplified, their identification was done with universal primer sets (1F / 1R, )with expected band of( 328 bp).Of sixty eight universal primer based RT - PCR positive samples tested for serotype A - 1C562(865bp), O - 1C272( 635bp ) and Asia1 - 1C505(911bp) serotypes primers, 19(27.9%) samples were found to be serotype A - 1C562 positive and 26(38.2%) were serotype O - 1C272 positive and nil for Asia1 - 1C505 serotype. The frequency of foot - and - mouth disease viral genome presence in cattle had significant differences concerning the sex (P<0.05) while the difference among age groups was highly significant (P < 0.01) in case of universal gene and not significant(P>0.05) in case of all serotypes genes. Depending on the sex effect, the rate of RT - PCR positive results of universal gene and O - 1C272 serotype gene were higher in females (87.5 and38.8% respectively ),while A - 1C562 serotype RT - PCR positivity rate was higher in males (31.6% ).Different rates of RT - PCR positivity was observed in the two age groups of cattle and the second age group(>4 - 8 year) showed higher rate of positivity as a follow : (universal primer (90.7%),O - 1C272 serotype primer( 40.8%) and A - 1C562 serotype primer(34.7% ).In the studied regions of Basrah province , the FMDV genes were significantly(P<0.05)distributed. The rates of universal gene appearance were100, 93.3 and 83.3%of cattle in Zubair ,Qurna and Shutt - Alarab respectively. The serotype A - 1C562 was distributed in higher rate ( 100 and 40%) of Zubair and Al - Mdaina cattle respectively , while the serotype O - 1C272 was observed in higher distribution rate in cattle of Abi - Elkhasib (44.4%) and Qurna(42.9%), so there is significant differences ((P<0.05) according to distribution of serotypes of FMDV of regions of Basrah province.

عزل وتشخيص جرثومة ال (Pasteurella multocida) من الابقار والاغنام وتصنيف انماطها المصلية باستخدام تقنية تفاعـل السلسلة المتبلمرة == Isolation And Diagnosis Pasteurella Multocida From Cattle And Sheep And Serotyping Classification Using Polymerase Chain Reaction Technique

Author name: جنان ناظم صادق العبيدي
Supervisor name: عدنان حمد عبيد الحمداني | قاسم حليم كشاش
General topic: Veterinary Medicine
Specific topic: Microbiology - Biotechnology
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة جمع 150 نموذج من الرئات المصابة والمسحات الانفية ومسحات اللوزتين من الابقار والاغنام للمدة1 / 11 / 2010 ولغاية1 / /4 2011 من حظائر حيوانية ومجازر مختلفة في مدينة الديوانية. تم زرع العينات على ( وسط اكار الدم ووسط الماكونكي واكار تربتون الص | The study included the collection of (150) samples of infected lungs and nasal , tonsils swabs from cattle and sheep at the period 1/11/2010 to 1/ 4/2011 of farm animals and various massacres in the city of Diwaniya. Samples were cultured on the blood agar and MacConkey agar and Trypticase Soya agar then diagnosed after pure isolation of colonies of pasteurella multocida were diagnosied and identified using morphological and biochemical features. The results revealed that the total isolation percent of Pasteurella multocida was 82(54.6%) which distributed as 40(53%) and 42(56%) for cattle and sheep ,respectively.on the other hands ,the high percent of isolation were recoverd from infected lungs 16(64%) followed by tonsils 13(52%) then nasal swabs 11(44%) in respect with cattle ,while in sheep ,the percent were 17(68%) for infected lungs ,11(44%) for nasal swabs and 14(56%) for tonsils swabs The percent of P.multocida isolation in cattle was 28(51.8%) in age group (>2years)while it was 12(57.1%) in age group (<2years),where as these percents in sheep were 27(56.2%) and 15(55,5%) ,respectively. In male ,the percent of isolation were 22(45.8%) and 20(54%) in females in respect with sheep samples ,while it was 21(51.2%) and 9(55.81%) in cattle respectively ,there was no significant differences (p>0.05) between isolation percent according to species ,sex and age of animals. The results of polymerase chain reaction (PCR) as confirmatory test for P.multocida isolates after DNA extraction and amplification with specific primers named KMT - 1 showed asingle band amplified DNA with amolecular weight of 460 bp. In purpose of serotyping the isolates of Pasteurella multocida by using PCR, the specific primer of capsule ( CAPA, CAPB, CAPD, CAPE, CAPF) were amplified the results revealed that the serotype (B) was the dominat in cattle , with molecular weight (760pb) while type ( A) the dominat in sheep with molecular weight (1044pb). In Coclusion , the result of molecular diagnosis of P.multocida serotyping by using PCR revealed ahigh specificity (84.5 %) sensitivity (97.05%) in Comparision with routine diagnostic of this bacteria in cattle and sheep.

تشخيص وتوصيف جراثيم الاشريشيا القولونية والمكورات العنقودية الذهبية ذات الاحتمالية المرضية المعزولة من الحليب الخام للابل والجاموس بالطرق الاعتيادية والجزيئية == Identification And Molecular Characterization of Potential Pathogenic Staphylococcus Aureus And Escherchia Coli Isolated From She - Camel And Buffalo Raw Milk

Author name: اسراء تلفان عناد
Supervisor name: باسل عبد الزهرة عباس | بسام ياسين خضير
General topic: Veterinary Medicine
Specific topic: Microbiology - Biotechnology
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: English
University location: Basrah
First pages:
Abstract: تم جمع 430 عينة ,215 عينة من حليب الجمال الخام جمعت من مناطق مختلفه من محافظة البصرة ومحافظات بالمنطقة الجنوبية للعراق و215عينة من حليب الجاموس الخام جمعت من مناطق مختلفة من محافظة البصرة خلال فترة ثمانية اشهر(اب 2012 - اذار 2013) حيث تم جمع الحليب يدويا | During a period of eight months (August 2012 to March 2013), a total of 430 samples were collected from 215 camel milk samples, 215 buffalo milk samples from different regions in Basrah city and other cites in southern area in Iraq. All specimens were screened for the presence of E. coli and S.aureus by cultured on MAC, EMB and MS agar media.A total of 51 (11.8 %) of suspected E. coli isolates were obtained; 23 (10.6%) from camel milk samples and 28 (13.0%) from buffalo milk samples. The study showed higher occurrence of E. coli in buffalo milk samples than camel milk samples. All suspected E. coli isolates were tested with Api 20 and 35(68.6%) were confirmed as E. coli. Staphylococcus aureus were also isolated as suspected staph, A total of 39 (9.06) were obtained; 17 (7.9) from camel milk samples and 22 (10.2) from buffalo milk samples. The study showed higher occurrence of S.aureus in buffalo milk samples followed by camel milk samples. After test the suspected staph with api test appear 27 (69.2) were confirmed as S.aureus.All the isolates of E.coli and S. aureus were tested to at least 12 antibiotics to which they were subjected. All the isolated bacteria (100%) of E. coli isolates were susceptible to gentamycin,imipenem and amikacin but resistant (100%) to amoxicillin, trimethoprim, azithromycin ,amoxicillin/clavulanic acid, cephalothin, cefixime and cefoxitin. Some of them (50%) were resistance to nalidixic acid. Three isolates 75% were resistance to tetracycline whereas 75% were susceptible to ciprofloxacin, and nitrofurantoin. Intermediate susceptibility to ciprofloxacin ,nalidaxic acid , nitrofurantoin and tetracyclin was observed among 25% of the E. coli isolates whereas 25% showed resistance to nalidixic acid. Therefore, all these isolates were considered to be multidrug resistant.E. coli were further examined by PCR technique using two pairs of primers to amplify both itsf and pai genes. The results revealed that all (85.7%) of the isolates were positive for itsf gene, 57.1% of isolates yielded amplification products with Pai - PCR gene. Staph aureus examined to presence of mecA gene by technique using pairs of primers and it was found that 51.8% of strain contain that gene. Clustalw multiple sequence alignment of icd gene of E.coli and arcC gene of S.aureus isolates and three previously reported E.coli and S.aureus was done.

تطبيق طريقة التحول الوراثي في نقل صفة انتاج مضاد حيوي من بكتريا Streptomyces sp الى Escherichia coli pBR322 == Application Of Transformation Method In Transfer Of Antibiotic Production Property From Streptomyces Sp. To Escherichia Coli pBR322

Author name: مرتقب يونس عبيد الحجاج
General topic: Veterinary Medicine
Specific topic: Microbiology - Biotechnology
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language: English
University location: Basrah
First pages: