Share
تشخيص وتوصيف جراثيم الاشريشيا القولونية والمكورات العنقودية الذهبية ذات الاحتمالية المرضية المعزولة من الحليب الخام للابل والجاموس بالطرق الاعتيادية والجزيئية == Identification And Molecular Characterization of Potential Pathogenic Staphylococcus Aureus And Escherchia Coli Isolated From She - Camel And Buffalo Raw Milk
Author name:
اسراء تلفان عناد
Supervisor name:
باسل عبد الزهرة عباس | بسام ياسين خضير
General topic:
Veterinary Medicine
Specific topic:
Microbiology - Biotechnology
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language:
English
University location:
Basrah
First pages:
21T492 - p.pdf
Abstract:
تم جمع 430 عينة ,215 عينة من حليب الجمال الخام جمعت من مناطق مختلفه من محافظة البصرة ومحافظات بالمنطقة الجنوبية للعراق و215عينة من حليب الجاموس الخام جمعت من مناطق مختلفة من محافظة البصرة خلال فترة ثمانية اشهر(اب 2012 - اذار 2013) حيث تم جمع الحليب يدويا | During a period of eight months (August 2012 to March 2013), a total of 430 samples were collected from 215 camel milk samples, 215 buffalo milk samples from different regions in Basrah city and other cites in southern area in Iraq. All specimens were screened for the presence of E. coli and S.aureus by cultured on MAC, EMB and MS agar media.A total of 51 (11.8 %) of suspected E. coli isolates were obtained; 23 (10.6%) from camel milk samples and 28 (13.0%) from buffalo milk samples. The study showed higher occurrence of E. coli in buffalo milk samples than camel milk samples. All suspected E. coli isolates were tested with Api 20 and 35(68.6%) were confirmed as E. coli. Staphylococcus aureus were also isolated as suspected staph, A total of 39 (9.06) were obtained; 17 (7.9) from camel milk samples and 22 (10.2) from buffalo milk samples. The study showed higher occurrence of S.aureus in buffalo milk samples followed by camel milk samples. After test the suspected staph with api test appear 27 (69.2) were confirmed as S.aureus.All the isolates of E.coli and S. aureus were tested to at least 12 antibiotics to which they were subjected. All the isolated bacteria (100%) of E. coli isolates were susceptible to gentamycin,imipenem and amikacin but resistant (100%) to amoxicillin, trimethoprim, azithromycin ,amoxicillin/clavulanic acid, cephalothin, cefixime and cefoxitin. Some of them (50%) were resistance to nalidixic acid. Three isolates 75% were resistance to tetracycline whereas 75% were susceptible to ciprofloxacin, and nitrofurantoin. Intermediate susceptibility to ciprofloxacin ,nalidaxic acid , nitrofurantoin and tetracyclin was observed among 25% of the E. coli isolates whereas 25% showed resistance to nalidixic acid. Therefore, all these isolates were considered to be multidrug resistant.E. coli were further examined by PCR technique using two pairs of primers to amplify both itsf and pai genes. The results revealed that all (85.7%) of the isolates were positive for itsf gene, 57.1% of isolates yielded amplification products with Pai - PCR gene. Staph aureus examined to presence of mecA gene by technique using pairs of primers and it was found that 51.8% of strain contain that gene. Clustalw multiple sequence alignment of icd gene of E.coli and arcC gene of S.aureus isolates and three previously reported E.coli and S.aureus was done.
