الكشف السيرولوجي والجزيئي لفايروس مرض الحمى القلاعية في الابقار في محافظة البصرة == Serological And Molecular Detection of Fmdv In Cow of Basrah Province
Author name:
زينب مجيد سالم الكلي
Supervisor name:
عدنان موسى الروضان
General topic:
Veterinary Medicine
Specific topic:
Microbiology - Biotechnology
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language:
English
University location:
Basrah
First pages:
21T508 - p.pdf
Abstract:
اجريت هذه الدراسة في6 مناطق مختلفة ( شط العرب والزبير والقرنه وابي الخصيب ومركز البصره والمدينه) ممثله لجميع اجزاء محافظة البصره خلال الفتره من شهر تشرين الاول من العام 2012 الى شهر تموز من عام 2013 ولتحديد نسبة انتشار مرض الحمى القلاعيه في الابقار استخد | This study was conducted on local cattle from 6 different regions (Shutt - Alarab,Al - Zubair, Al - Qurna, Abi - Elkhasib, Basrah center and Almdaina) covering all parts of the Basrah province during the period from October 2012 to July 2013. In order to determine the prevalence of foot and mouth disease in bovine species Enzyme Linked Immune Sorbent Assay ( ELISA) and reverse transcriptase polymerase chain reaction techniques( RT - PCR) were used in the testing of serum ,saliva and vesicular fluid samples collected from clinically diseased cattle, moreover this study was also mentioned some clinical aspects of the disease.Diseased animals showed the following clinical signs : depression(92.1%),sever salivation(90.9%) ,vesicles in mouth(90.3%), fever(89.1%) , conjunctivitis(87.9%) and smacking of lips in higher rates( 80.6%).While the vesicles on teat(12.7%), lameness(36.9%)and vesicles on feet (45.5%)appeared in lower rates.The overall prevalence of this disease was(72.7%.).Of 165 serum samples subjected to nonstructural protein (NSP) ELISA screening test (ELISA CHEKIT FMD - 3ABC Bo - Ov ELISA Test ) , 120 were interpreted as positive representing (72.7%.) while the other 45samples were negative representing 27.3%.The seropositivity significantly associated with age groups (p<0.01) and sex (P < 0.01).Depending on sex of these animals , the higher seropositivity rate was observed in females (78.6%).Concerning the age of tested cattle the animals of 2nd age group (>4 - 8 years) appeared in higher rate (84.8 %) of seropositivity followed by the rate (51.7% ) of 1st age group animals(<1 - 4 years).The sero - positivity against FMDV was non significantly different(>P 0.05)among the cattle in Basrah districts and highest sero - prevalence rate was observed in cattle of Basrah center (100%), Al - Mdaina (77.8%), Shut - Alarab(76.3%) and Abi - Elkhasib (70%).The lower sero - positivity rate was observed in Qurna(58.8%)and Zubair (50%) cattle.RT - PCR detection of FMDV for primary and serotype specific diagnosis was used. of eighty three clinically positive samples including, mouth vesicles, saliva and serum samples tested by RT - PCR, only 68(81.9%) were successfully amplified, their identification was done with universal primer sets (1F / 1R, )with expected band of( 328 bp).Of sixty eight universal primer based RT - PCR positive samples tested for serotype A - 1C562(865bp), O - 1C272( 635bp ) and Asia1 - 1C505(911bp) serotypes primers, 19(27.9%) samples were found to be serotype A - 1C562 positive and 26(38.2%) were serotype O - 1C272 positive and nil for Asia1 - 1C505 serotype. The frequency of foot - and - mouth disease viral genome presence in cattle had significant differences concerning the sex (P<0.05) while the difference among age groups was highly significant (P < 0.01) in case of universal gene and not significant(P>0.05) in case of all serotypes genes. Depending on the sex effect, the rate of RT - PCR positive results of universal gene and O - 1C272 serotype gene were higher in females (87.5 and38.8% respectively ),while A - 1C562 serotype RT - PCR positivity rate was higher in males (31.6% ).Different rates of RT - PCR positivity was observed in the two age groups of cattle and the second age group(>4 - 8 year) showed higher rate of positivity as a follow : (universal primer (90.7%),O - 1C272 serotype primer( 40.8%) and A - 1C562 serotype primer(34.7% ).In the studied regions of Basrah province , the FMDV genes were significantly(P<0.05)distributed. The rates of universal gene appearance were100, 93.3 and 83.3%of cattle in Zubair ,Qurna and Shutt - Alarab respectively. The serotype A - 1C562 was distributed in higher rate ( 100 and 40%) of Zubair and Al - Mdaina cattle respectively , while the serotype O - 1C272 was observed in higher distribution rate in cattle of Abi - Elkhasib (44.4%) and Qurna(42.9%), so there is significant differences ((P<0.05) according to distribution of serotypes of FMDV of regions of Basrah province.
