Author name:
بيداء ريحان علي الموسوي
Abstract:
The present study was designed search of some physiological and histological effects of the venom of Echis carinatus sochureki (Said Dakhil snake) in male and female rats, and the role of antivenom (anti - venom) in the treatment of these effects. The while study was divided into two parts, the first part included the effect of [0.04 mg/kg (the second group) and 0.08 mg/kg (the third group)] of venom in rats. While, the second part related with the effect of [0.25ml/animal (the second group) and 0.5ml/animal (the third group)] antivenom in male and female rats treated with 0.04mg/kg and 0.08mg/kg of venom. After 24 hours of injection, blood samples collected for the hematological and biochemical parameters, and taking the right epididymis and epididymis left removed to examine the count and maliformations of sperm. Also, liver, kidney, testes and ovary were collected for sections. The results of the present study showed a significant decrease (P≤0.05) in RBC in the third group (0.08) mg / kg when compared with the first group (control) and second group (0.04) mg / kg. Also was there a significant decrease in HCT and MCV in the second and third groups compared with the first group, while there was a significant increase in the platelets of the third group compared with the first and second groups in in both sexes. The results indicated a significant decrease in RBC in the second and third groups compared with the first group, also a significant decrease in HCT in the second and third groups compared with the first group. The results showed a significant increase in the HCT in the second group compared with the third group. The results showed a significant decrease in the MCV in the third gr oup compared with first and second groups in female rats. The present study (P≤0.05) showed a significantdecrease in total WBC, lymphocytes and neutrophils in the second groups (0.04) mg / kg and the third (0.08) mg / kg, and a significant decrease in monocytes, acidiophils and basophils of the second and third groups compared with the first in the male rats. The results indicated a significant decrease in neutrophil in the second and third groups compared with the first group, and a significant decrease in lymphocytes, monocytes and acidiophils of the third group compared with the first and second groups in the female rats. The present study showed a significant increase (P≤0.05) in the level of glucose and urea in the third group (0.08) mg / kg compared with the first (control) and second (0.04) mg / kg groups. There was a significant increase in creatinine and albumin in the second and third groups compared with the first group, also a significant decrease in the total protein, cholesterol and HDL in the second and third groups compared with the first group.There was a significant decrease in the level of TG, LDL and VLDL in the third group compared with the first and second groups, The results showed a significant decrease in ALT and AST in the third group compared with the first and second groups, and a significant increase in the ALP in the third group compared with the first and second groups in the male. The hormonal study showed a significant decrease (P≤0.05) in the level of FSH, LH, testosterone and estrogen in the second and third groups compared with the first group in male and female rats. The present study a significant decrease (P≤0.05) in the count of sperms in the second and third groups compared with the first group, while, there was a significant increase in the maliformations of sperms in the third group compared with the first and second groups. The present study showed a significant decrease (P≤0.05) in the level of immune globulins (IgA - IgG - IgM) in the second and third groups compared with the first group in both sexes. The results of the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated with (0.04) of snake venom showed a significant decrease in RBC and HCT in the second and third groups compared with the first groups,and there was a significant increase in the MCV in the second and third groups compared with the first group in male. The study results showed a significant increase in RBC, HCT and MCV in the second and third groups compared with the first group in the female rats.The current study showed a significant decrease (P≤0.05) in total WBC of the second group compared with the first and third groups, and a significant decrease in the lymphocytes and monocytes in the second and third groups compared with the first group, also the results showed a significant increase in total WBC in the second and third groups compared with the first in the male. Also, found a significant decrease in lymphocytes and monocytes in the secon d and third groups compared with the first, it was observed a significant rise in monocyte in the first group compared with the second and third groups. The results showed a significant increase in the percentage of granular white blood cells in the second and third groups compared with the first group in the female rats.Results of the present study indicated a significant decrease (P≤0.05) in the level of glucose, urea, albumin and total protein in the third group compared with the first and second groups, and there was a significant increase in the level of cholesterol HDL - VLDL in the third group compared with the first and second groups, also a significant decrease in TG in the second group compared with the first and third groups, The results showed a significant decrease in the level of HDL in the second and third groups compared and second groups with the firstgroup. the results showed a significant increase in the level of albumin and total protein in the third group compared with the first and second groups, and the results indicated a significant in increase cholesterol level, LDL and VLDL in the third group compare with the first and second groups, There was a significant decrease in the level of TG in second group compared with the first and third groups, and a significant decrease in the level of HDL in the second and the third groups compared with the first group in the female rats.The results indicated the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated (0.08) mg / kg of snake venom, showed a significant decreased (P≤0.05) in RBC, HCT , MCV, lymphocyte and monocyte in the second and third groups compared with the first group, a significant decrease MCH in the second group compared with the first and third groups in male rats, showed a significant decrease in RBC HCT and MCV in the second and thirdgroups compared with the first group.The present study showed a significant decrease (P≤0.05) in the level ofglucose and urea, ALT and ALP in the third group compared with the first and second groups, also a significant decrease in the level of creatinine, LDL, VLDLand AST in the second and third groups compared with the first group, and asignificant increase in the level of albumin, total protein and HDL in the thirdgroup compared with the first and second groups. a significant increase in levelof cholesterol and TG in the second and third groups compared with the firstgroup in the male rats, While showed a significant decrease in level of glucose,urea and ALT in the third group compared with the first and second groups,there was a significant decrease in the level of creatinine, LDL, VLDL, AST and ALP in the second and third groups compared with the first group, a significant increase in albumin and total protein in the group third compared with the first and second groups, while a significant increase in level of cholesterol, TG and HDL in the second and third groups compared with the first group in female.The histological results showed changes in the liver of male and female rats treatment I n venom snake and constrictions consisted obtaining inflammation and blood congestion and enlarged nuclei of hepatic cells and degeneration in the cytoplasm and necrosis of liver cells, as well as, changes in the kidney tissue such as having a bleeding and hyperplasia and congestion blood and inflammation and analyze the entire glomerulus and necrosis of the cortical cells.The current study showed the incidence and clear changes in testicular tissue treatment venom snake of obtaining vascular congestion and analyze the interconnection fabric between the tubule and the decay of some nuclei and abnormalities tubule and testicular and shrinking and analyzes and the presence of multiple nuclei giant cells in the wall of tubule.The current study for histological changes in the ovary as sections showed the presence of large numbers of corpus luteum and the decrease numbers of ovarian follicles and the disappearance of the evolutionary stages of ovarian cysts.
