التغيرات النسجية والنسجية الشكليائية القياسية لعقار الليفيتراسيتام في بعض اعضاء الجهاز التكاثري في الفئران البيض == Histological and Histomorphological Changes of Levetiracetam Drug in Some Reproductive Organs in White Mice
Author name:
فائزة جبار جودة الربيعي
Supervisor name:
نهلة عبد الرضا البكري
General topic:
Biology
Specific topic:
Zoology - Histology
Degree:
Doctorate
University:
University of Baghdad
Language:
Arabic
University location:
Baghdad
First pages:
24T3398 - p.pdf
Abstract:
This study was conducted to study the effects of Levetircetam (LEV) drug at concentration of 350 mg/kg/day body weight on selected organs of male and female reproductive system in albino mice, Mus muculus (Swiss mice). The animals divided into three treated groups, the first group treated once a day for 14 days, the second group treated once a day for 30 day and the third group treated once a day for 45 days, in addition to 3 control groups one for each treated group.The results of the present study declare the following : • Selected organs of male reproductive system (testes, caput (head) epididymis and cauda (tail) epididymis).The results revealed significant degrease (P<0.05) in the percentages of the weight of testes, caput (head) epididymis and cauda (tail) epididymis in treated animals for 14, 30, 45 days respectively in comparison with control group. The results also showed significant decrease (P<0.05) in percentages spermatogonia of the treated animals, when compare with control group. The percentages of the primary spermatocyte, secondary spermatocyte and spermatid increased significantly (P<0.05) in all three treated groups when compare with control groups and significant decrease (P<0.05) in percentage of spermatozoa, number of Sertoli cells, Leydig cells and diameter of Leydig cells nuclei. The mean diameter and thickness of somniferous tubules and height of epit helial cells and thickness of the tubules in the caput (head) and cauda (tail) epididymis in treated animal when compare with control group.The results of present study also showed that treatment with drug lead to histopathological changes in testes of animals treated with drug when compared with control groups such as degeneration, necrosis of spermatogenic cell and vacuolated of some seminiferous tubules, hypotrophy of interstitial and accumulation of spermatid at different stages of spermatogenic cells in lumen of tubules and degeneration of some of Leydig and separation of Sertoli cells from the basement membrane. Also results of percent study revealed some histopathological changes in caput (head) and cauda (tail) epididymis of treated animals with drug when compared with control groups, these change represented by degeneration some of epithelial cells and detachment of basement embrane and appearance of phagocytes in lumen of tubules and degeneration of epithelial cells and appearance of vacuoles between epithelial cells in addition to the damage of tubules. • Selected organs of female reproductive system significant increase (P<0.05), mean weight of ovary, oviducts and uterus of treated animals with drug for 14, 30, 45 days in comparison compared with control groups.Significant decrease (P<0.05) number of primary follicles and significant increase (P<0.05) in number of secondary follicles and significant increase (P<0.05) number of mature follicles of treated animals with drug about 14, 30, 45 days when compared with control group, significant increase (P<0.05) in number follicular cysts and corpus lutues of treated animals with drug when compared with the control groups, and significant decrease (P<0.05) diameter of growth follicles and corpus luteus and significantlyincrease (P<0.05) in diameters follicular cysts of treated animals with drug when compared with control groups. The results revealed some histopathological changes in ovary of treated animals with drug for when compared with the control groups, these changes represented by hypertrophy of cortex, degeneration of granular layer and accumulated some of cells in the middle of follicular lumen, degeneration in ovum and situated at the central position with increasing in the number of follicular cysts, separated of ovum from columns ovum, appearance of some abnormal ovum in some of follicles of ovaries.Significant decrease (P<0.05) in height and numbers of mucus folds in ampula and isthmus regions in oviduct of treated animals with drug when compared with control groups in addition to replacement of the ciliated columnar cells by cuboidal cell with the degreasing in the density of the cilia and the height of the secretory cells in addition hemorrhage, necrosis, hypoplasia and death of the columnar epithelial cells of the mucus folds, with the appearance of vacuoles, phagocytes in the center of the lumen,hypertrophy and hyperplasia in the tunica muscularis. Results also showed significant decrease (P<0.05) in numbers of endometrial glands of treated animals with drug when compared with control and significant increase (P<0.05) number of blood of treated animals with drugs when compared with control group and appearance significant increase (P<0.05) in high of epithelial cells of endometrium and thickness of lamina propria of three treated animals with drugs when compared with control group.The results revealed a histopathological changes in endometrium of treated animals with drug when compared with control such as hyperplasia and hypertrophy the epithelial layer and lamina propria and atrophy of endometrial glands and hyperplasia in epithelial of endometrial gland and detached basement membrane in some of them
