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التاثير الوقائي لمستخلص بذور نبات البلنكو Lallemantia royleana في حماية الكبد من سمية عقار الريفادين في ذكور الفار الابيض == Hepatoprotective Effect of Lallemantia royleana Seeds Extract Against Rifadin Toxicity in Male Albino Mice
Author name:
محمد فاضل عبد الحسين
Supervisor name:
زينب فاضل شويت الاسدي
General topic:
Biology
Specific topic:
Zoology - Histology
Degree:
Master
University:
University of Baghdad
Language:
Arabic
University location:
Baghdad
First pages:
24T3415 - p.pdf
Abstract:
This study aimed to focus on the hepatoprotective effect of Lallemantia royleana seeds extract against rifadin hepatotoxicity in experimental mice.The study was conducted on swiss albino male mice (40), there age ranged from 5 - 8 weeks with weight of 25 - 30 gm that divided into four animals groups : • First group orally administered with (0.1 ml) normal saline (0.9%) for 28 days as control group. • Second group orally administered with ( 0.1 ml) rifadin (1.5 mg/kg/day) for 28 days.• Third group orally administered with (0.1 ml) alcoholic extract of Lallemantia royleana seeds (1%) for 28 days . • Forth group was also orally administered with seeds extract alone for 5 days and with (0.1 ml) seeds extract and rifadin for 28 days, so the total period of this group is 33 days. Blood samples ware collected from experimental animals to measured liver function enzymes (ALT,AST,ALP) levels, liver samples were also collected for histopathological and immunohistochemical studies.Results showed there was animals activity changes in groups especially in drug administered group compared with other groups, which represented by lethargy, introversion,the tendency to sleep, hyperventilation, in addition to change the faeces color and urine color, and these behavioral changes noted after 15 days from experiment began.The statistical analysis of the data showed significant increase (P<0.05) in AST, ALT,ALP levels in blood serum of animal group administered with rifadin (662.04±8.26, 131.575±3.45, 197.00±6.58) respectively compared withcontrol group ( 25.2 ± 21.562 , 445..2±2522, 2.543±6524 ) significant decrease (P<0.05) was also noticed in AST,ALT level in seeds extract group( 2..563±.54., 47.480±1.93) and seeds extract - drug group ( ...531±.5.1, 85.033±2.33) compared with drug group, while significant increase (P<0.05) in ALP enzyme level in animals group administered with seeds extract alone (4..5..±.533 ) compared with drug group.The histopathological changes in liver tissue of experimental grups, was represented by loss of normal architecture of liver tissue and radial organization of hepatic cords in centrilobular region and peripheral regions in animals administered with drug, vaculation and death of hepatic cells, congestion (moderate/ severe) in central veins, and few infiltration of mononuclear leukocyte in centrilobular region. In animals administered with seeds extract - drug group was preserved the hepatic cords radial organization especially surrounding the central vein, congestion (mild/ moderate) in central veins, alarge numbers of balone - shape cells with vaculated hepatic cells, and the histological structure in animals administered with seeds extract was approached to control group, that was no dissociation in liver tissue and hepatic cords radially organized around central vein, and mononuclear leukocyte infiltration in centrilobular region and the adjacent region of portal traid.The immunohistochemical test result revealed significant increase (P<0.05) in apoptotic index and in the cells with Bax protein that stimulating the process of apoptosis possitive reaction in drug administered animals group )%665.22±2542( compared with other experimental groups, also the results revealed significant decrease (P<0.05) in apoptotic index and the cells with Bax protein possitive reaction in animals administered with seeds extract and seeds extract - drug compared with drug group, ( 251.2±2521, 25422±2522) % respectively.
