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تقييم وعزل فيروس الانفلونزا للانسان ودراسة الاستجابة المناعية في الجرذان التجريبية == Evaluation And Isolation of Human Influenza Virus And Studying Their Immune Response In Experimental Rats

Author name: فاديه مهدي مسلم العبيدي
Supervisor name: عبد المجيد عبد الله السعدي | يونس عبد الرضا الخفاجي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: University of Kufa - College Of Science - Department Of Biology
Language: English
University location: Najaf
First pages:
Abstract: This study has been conducted for the first time in Najaf / Iraq, and the study included the preparation of a local inactivated vaccine (whole and subunit).The number of cases infected with seasonal influenza virus were 647 cases out of one thousand suspected case. They were distributed into eleven groups. Seasonal influenza virus was detected by two diagnostic methods (rapid test device and real time PCR).The present study has reflected that the most diagnosed cases infected with seasonal influenza virus during the period extended from March 26, 2012 up to April 30, 2013 represented by were type A (H3N2) (283) cases. Whereas H1N1 (148) cases, H3N2+H1N1, (56) cases, H1N1 + H3N2 + B were (30) cases and 130 cases were infected with type B.Current study also revealed that age group of 46 - 60 years old infected with seasonal influenza virus were (159) case including (368) male and females were (279) of different infected group..The spread of seasonal influenza virus in Najaf varies from one region to another depending on the population density. Clinical cases were collected randomly during the months of the year. It has been found that the spread of disease has something to do with temperature and humidity as high humidity and low temperature lead to increased spread of the disease because of their relationship at the stage of the spread of epidemic influenza that spread from Dec 26, 2012 and up to April 1, 2013 in addition to expatriates infected..Two techniques were used for isolation of seasonal influenza virus first inoculation of embryonated chicken egg and secondly chicken fibroblast cell culture.It was found that at the embryonated egg lethal dosage (ELD 50 / 0.5 ml) (109.6) whereas tissue culture infective dosage (TCID50 / 0.1 ml) was (109.3) of (H1N1+ H3N2) and type (B) virus..Virus was purified by two ways that are depending on the vaccine type. The gamma irradiation that dosage used for inactivating vaccine of the purified virus which was extracted from allantoic fluid and tissue culture was (5.702) mSv/h for subunit virus vaccine type whereas gamma irradiation dosage for complete (whole) virus vaccine was (8.816) mSv/h. The results proved that immunoglobulins (IgM, IgA and IgG) levels as well as (IL - 17, IL - 10 and INF - 1?) indicated significant differences among vaccinated, infected and control groups.Subunit and whole virus vaccine extracted from chicken fibroblast cell culture are considered the best of derived from virus propagated in embryonated chicken eggs.Subunit virus vaccine is better than the whole virus vaccine derived from virus propagated in embryonated chicken eggs and extracted from chicken fibroblast cell culture.The current study also reflected that locally prepared virus subunit vaccine and purified whole virus killed vaccine capable of inducing humoral and cellular immune response in experimentally vaccinated rats..Histopathological changes appeared clearly on tissue sections (lung, trachea, heart, liver, spleen, kidney) of the infected group as compared with control and the vaccinated groups.

دور الفيروس المضخم للخلايا CMV في مرضى التهاب الكبد الفيروسي ذاتي المناعة في حالات التهاب الكبد المزمن نمط B == The Role of CMV In Autoimmune Hepatitis Among Chronic Cases of Hbv

Author name: هـدى جميل باقر الخلخالي
Supervisor name: محمد عبود محسن | محمد عبد كاظم السعدي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: University of Kufa - College Of Science - Department Of Biology
Language: English
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تشخيص التهاب الكبد الفايروسي نمط (ب) وتشخيص التهاب الكبد ذاتي المناعة في المرضى المصابين بالتهاب الكبد.ودور الاصابة بالفايروس المضخم للخلايا البشرية CMV في حث التهاب الكبد ذاتي المناعة.من مجموع 360 حالة مشبه بها جمعت من مستشفى ال | This study aimed to investigate the existence of hepatitis B virus and autoimmune hepatitis in hepatitis B patients as well as to detect the role of cytomegalovirus in the induction of AIH disease. A total of 360 suspected cases were collected from Center Health laboratory/Al - Hakeem Hospital, and AL - Sadder medical city in AL - Najaf city, during the period from January (2013) to August (2013). Only 76 were seropositive hepatitis B (55 males and 21 females with age ranging 11 - 72 years).In addition, 15 healthy individuals without any evidence of chronic inflammatory disease were depended on the control group, age ranging 21 - 50 years.Blood samples were collected from patients and healthy controls were tested for HBsAg and Anti - HBc Ab using Enzyme Linked Immunosorbent Assay (ELISA) technique to investigate hepatitis B seropositive and chronic hepatitis B respectively. For investigated AIH disease was performed depending on the Line Immune Assay technique. While for detection CMV were initially identified by serological technique (ELISA, MiniVIDAS) to detect anti - CMV IgM and anti - CMV IgG; then confirmed employment molecular technique using Real Time Polymerase Chain Reaction for the detection the presence of DNA of CMV. Samples were collected from patients and control to estimation immunological level (C3, C4, IL - 10 & TFN - ?) by using ELISA and radial immunodiffusion method.The results showed that 76 HBsAg seropositive in all age groups but the age group 44 - 54 year revealed high significance (p<0.05) than other age groups. While 35 out of 76 seropositive with HBc Ab, the age group 55 - 65 years showed high significance (p<0.05) than other age groups, and males more infection than females. The result also revealed that the AIH disease was 5 out of 76 patients infected with Type 1 AIH. Included 2(40%) have demonstrated the infection HBsAg positive with reactivation CMV While 3(60%) infected with HBsAg only.The results showed that 68 out of the 76 samples were positive for anti - CMV IgG antibodies, and 4(5.26%) samples were positive for anti - CMV IgM. While the MiniVIDAS test results showed 73 out of the 76 samples was positive for anti - CMV IgG antibodies. 2(2.6%) were positive for anti - CMV IgM antibodies. The results of the Real - Time PCR revealed that DNA of CMV were detected in 23 out of 76 patients were found in all age groups with viral loads ranging from (0.24 - 1730000) Copies/ml, and the results of controls group in Real - Time PCR were CMV negative. The results of cytokines (TNF - ?) showed a high significance (P<0.05) elevation in the serum of all patients than control (419.3 ± 27.8) pg/ml, and the results that AIH showed increase in cytokine level was (1218.2±44) pg/ml than other patients. Whereas chronic hepatitis B patients recorded high significance (P<0.05) in level (IL - 10) was (901.5±22.2) pg/ml than other patients and control (373 ±30.3) pg/ml. According to sex no - significance difference between males and females in results of cytokines profile (IL - 10, TNF?). Complement fraction C3 decreased in all patients compared to those healthy control, while the AIH patient recorded high significance (P<0.05) 142.2±8 mg/dI than the other patients. In regard to C4 was revealed normal level in all patients compared with control groups while in the AIH revealed high significance (P<0.05) was 41.7±5.1 mg/dI compared with other patients and no - significance difference (P<0.0) between males and females in level of C3and C4.The overall finding results showed that the activation cytomegalovirus with hepatitis B virus Contribute to the induction of AIH and cause immune suppressor for them by increase and decrease many immune factors.

التحري عن بعض المؤشرات المناعية وارتفاع نسبة وجود الفيروس HCMV في المرضى المصابين بالفشل الكلوي == Assessment of Some Immunological Markers And Viral Load For Hcmv In Patients With Renal Failure

Author name: احمد جاسم شوالة الخويلدي
Supervisor name: مهدي حسين محيل العمار | زياد متعب الخزاعي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: University of Kufa - College Of Science - Department Of Biology
Language: English
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة على 150 مريضا الراقدين في مستشفى الصدرالتعليمي ومستشفى الحكيم (قسم الكلى) في محافظة النجف خلال الفترة الممتدة من كانون الاول 2012 الى شهر اب 2013. وكان الغرض من هذه الدراسة بيان علاقة الفيروس المضخم للخلايا بمرض الفشل الكلوي، تراوحت اعما | This study was carried out on 150 renal failure patients, who were admitted to the kidney department in AL - Sadder Medical City and Al - Hakeem hospital in AL - Najaf governorate during the period from December 2012 to August, 2013. Subjects of this study were Investigated for the role of Cytomegalovirus among them, their age ranged between (1 - 88) years. Twenty four (age - and sex - matched) healthy individuals without any evidence of chronic inflammatory disease depended as control. All patients and control divided in four age groups. Blood and urine samples were collected from patients and control for immunological (IgM, IgG, C3, C4, IL - 6, IL - 10, IL - 12 & IFN - ?) by using ELISA and molecular study by RT - PCR, respectively. The obtained results showed that HCMV - IgG antibody was 100% for all cases, while IgM was 18.66% compared with that of control. Real time - PCR amplification for presence of HCMV DNA in urine samples revealed that HCMV genome were detected in 22(14.66%) of the 150 urine samples in all age groups, that distributed into 12(21.05%) with viral load ranged (20 - 543840) Copy/ml in females and 10 (10.75%) with viral load ranged (40 - 28050) Copy/ml for males. The results of cytokines profile showed a highly significant(P<0.05) elevation in patients than control. According to sex females appeared higher IFN - ?, IL - 6, IL - 10 ( 83.86, 82.67, 9.06 pg/ml, respetively) than males were (76.57 , 79.36, 6.51 pg/ml, respectively). Where's IL - 12 were recorded higher elevation in male (28.83 pg/ml ) than females (27.35 pg/ml). According to age groups 41 - 60 age group showed high level in IFN - ?, IL - 6, IL - 10 were (96.45, 90.40, 9.62) pg/ml , respectively.While IL - 12 appeared high level (37.43 pg/ml ) in age group 1 - 20 years Complement fractions C3, C4 decreased in all groups of patients compared to those of a healthy control. C3 was recorded (64.37 mg/dl) in females, and (70.24 mg/dl) in males and decreased to (63.41 mg/dl) in age group (1 - 20) years, while C4 decreased significantly among sex it was (21.06 mg/dl) in females and(24.22 mg/dl) in males, and down to (21.65 mg/dl) in age group (21 - 40) years.The overall finding results showed that HCMV are more prevalence among chronic renal failure patients and cause immune suppressor for them by increase and decrease many immune factors.

دراسة مصلية وبائية وجزيئية لفيروس التهاب الكبد نمط (ب) في قاطع الرصافة لمدينة بغداد == Seroepidemiological And Molecular Study Of Hepatitis B Virus In Al-Rusafa Sector Of Baghdad

Author name: ثائر كاظم فياض الحميري
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

التطبيق السريري لتقنية التفاعل التضاعفي لسلسلة الدنا وتقنية الزرع النسيجي في تشخيص التهاب السحايا المتسبب من الفيروسات المعوية == Clinical Application Of RT-PCR And Standard Tissue Culture Techniques For The Diagnosis Of Enteroviral-Associated-Meningitis

Author name: فائزة لفتة طعمة
Supervisor name: غيمة صادق محمد
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

دراسة بعض الجوانب الوبائية و المناعية حول الاصابة بالتهاب الكبد الفيروسي نمط B وC في محافظة القادسية == Study Some Epidemiological And Immunological Aspects On Viral Hepatitis Type B And C In Al-Qadisiyia Province

Author name: زياد متعب فجه سلطان الخزاعي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

التهاب الكبد الفيروسي نوع بي و سي دراسة وبائية مصلية و تحديد النمط الجيني في مدينة سامراء == Hepatitis B And C Virus Seroepidemiology And Genotypes In Samarra City

Author name: كيلان عبد الرزاق شاكر ياسين الدوش
Supervisor name: عبد الغني محمد السامرائي | عبد الكريم فتاح عمر
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: Tikrit University - College Of Science - Department Of Biology
Language: English
University location: Salahaddin
First pages:

بعض الحالات المناعية الوراثية لمتبرعي الدم السالبين للمستضد السطحي والموجبين لاضددا المستضد اللبي لفيروس التهاب الكبد الفيروسي البائي في محافظة البصرة – العراق

Author name: شوقي عبد الرزاق كاصد العبودي
Supervisor name: حيدر عبد الحسين مكلف الحمودي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:

الشيوع المصلي والجزيئي للاصابة بالفايروس الدوار عند الاطفال في مستشفى بنت الهدى التعليمي في محافظة ذي قار == Serological And Molecular Detection Of Rotavirus Infections In Children In Bint Al - Huda Teaching Hospital At Thi - Qar Province

Author name: نبراس عبد الكريم جبار
Supervisor name: سعد سلمان هميم | رزاق جميل الربيعي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: English
University location: Dhi Qar
First pages:

دراسة بعض الجوانب المناعية المرتبطة بالفيروس المضخم للخلايا في حالات الرفض الحاد للكلى المزروعة == Study Of Some Immunological Aspects Associated With Cytomegalovirus Acute Renal Graft Rejection

Author name: مي يوسف يعقوب ساعور
Supervisor name: غنيمة صادق محمد | عبد الوهاب عبد الرزاق الشيخلي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

اقتران مؤشرات التهاب المفاصل الرثواني و بعض المفاهيم المناعية مع التهاب الكبد الفايروسي نمط سي == Association Of Rheumatoid Arthritis Parameters And Some Immunological Aspects With Hepatitis C Virus

Author name: عباس عراك عباس
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

زيادة انتاج فايروس نيوكاسل في اجنة الدجاج باستخدام مادة الصوديوم دي اوكسي كوليت (SDC) وتاثيرها على انتاج الاضداد في الدجاج == Enhancment of Newcastle disease virus in embrionating chicken eggs using Sodium deoxycholate (SDC) and it’s effect on Antibody production in Chicken

Author name: ايمان عبد الرحمن محمود
Supervisor name: امنة نصيف جاسم | عبد اللطيف محمد احمد
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University of Baghdad - College Of Science For Girls
Language: Arabic
University location: Baghdad
First pages:

دراسة الية الاصابة ونقل الفايروسات بعزلات نوعي الفطرين Olpidium Brassicae و Olpidium Bornovanous وتاثيرهما على التراكيب التشريحية والخلوية للعائل == A Study of Olpidium Brassicae and Olpidium Bornovanous Fungi Varities Isolates in Fection Mechanism Viral Transmission and Their Influences on Anatomical and Cytological Structures of The Host

Author name: وفاء بخيت عبد الله الرابعي
Supervisor name: كاظم جاسم حمادي المنصوري
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University Of Basrah - Faculty Of Education
Language: Arabic
University location: Basrah
First pages:
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