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التحري عن بعض المؤشرات المناعية وارتفاع نسبة وجود الفيروس HCMV في المرضى المصابين بالفشل الكلوي == Assessment of Some Immunological Markers And Viral Load For Hcmv In Patients With Renal Failure
Author name:
احمد جاسم شوالة الخويلدي
Supervisor name:
مهدي حسين محيل العمار | زياد متعب الخزاعي
General topic:
Biology
Specific topic:
Microbiology - Viruses
Degree:
Doctorate
University:
University of Kufa - College Of Science - Department Of Biology
Language:
English
University location:
Najaf
First pages:
24T2663 - p.pdf
Abstract:
اجريت هذه الدراسة على 150 مريضا الراقدين في مستشفى الصدرالتعليمي ومستشفى الحكيم (قسم الكلى) في محافظة النجف خلال الفترة الممتدة من كانون الاول 2012 الى شهر اب 2013. وكان الغرض من هذه الدراسة بيان علاقة الفيروس المضخم للخلايا بمرض الفشل الكلوي، تراوحت اعما | This study was carried out on 150 renal failure patients, who were admitted to the kidney department in AL - Sadder Medical City and Al - Hakeem hospital in AL - Najaf governorate during the period from December 2012 to August, 2013. Subjects of this study were Investigated for the role of Cytomegalovirus among them, their age ranged between (1 - 88) years. Twenty four (age - and sex - matched) healthy individuals without any evidence of chronic inflammatory disease depended as control. All patients and control divided in four age groups. Blood and urine samples were collected from patients and control for immunological (IgM, IgG, C3, C4, IL - 6, IL - 10, IL - 12 & IFN - ?) by using ELISA and molecular study by RT - PCR, respectively. The obtained results showed that HCMV - IgG antibody was 100% for all cases, while IgM was 18.66% compared with that of control. Real time - PCR amplification for presence of HCMV DNA in urine samples revealed that HCMV genome were detected in 22(14.66%) of the 150 urine samples in all age groups, that distributed into 12(21.05%) with viral load ranged (20 - 543840) Copy/ml in females and 10 (10.75%) with viral load ranged (40 - 28050) Copy/ml for males. The results of cytokines profile showed a highly significant(P<0.05) elevation in patients than control. According to sex females appeared higher IFN - ?, IL - 6, IL - 10 ( 83.86, 82.67, 9.06 pg/ml, respetively) than males were (76.57 , 79.36, 6.51 pg/ml, respectively). Where's IL - 12 were recorded higher elevation in male (28.83 pg/ml ) than females (27.35 pg/ml). According to age groups 41 - 60 age group showed high level in IFN - ?, IL - 6, IL - 10 were (96.45, 90.40, 9.62) pg/ml , respectively.While IL - 12 appeared high level (37.43 pg/ml ) in age group 1 - 20 years Complement fractions C3, C4 decreased in all groups of patients compared to those of a healthy control. C3 was recorded (64.37 mg/dl) in females, and (70.24 mg/dl) in males and decreased to (63.41 mg/dl) in age group (1 - 20) years, while C4 decreased significantly among sex it was (21.06 mg/dl) in females and(24.22 mg/dl) in males, and down to (21.65 mg/dl) in age group (21 - 40) years.The overall finding results showed that HCMV are more prevalence among chronic renal failure patients and cause immune suppressor for them by increase and decrease many immune factors.
