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العلاقة بين التهاب الكبد الفايروسي نوع (C) والاعتلال الكلوي في مرضى فقر دم حوض البحر الابيض المتوسط == The Relationship Between HCV And Kidney Impairment In Beta Thalassemia Patient

Author name: سيف ياسين حسن

Supervisor name: سيف جبار

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

الدراسة الجزيئية لفايروس BK لنماذج من مرضى عراقيين مصابين بالتبول الدموي == A Molecular Study of the role of BK virus in hemorrhagic cystitis in a Sample of Iraqi patients

Author name: فائز كامل كتاب

Supervisor name: غانم عبود الموى

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

التعبير الجيني لبروتين الغشاء المتاخر للايبشتاين بار فايروس والجين الكابح للسرطان بي 53 في المرضى اللذين يعانون من سرطان الكلى == Epstein Barr virus LMP-1 expression and Tumour suppressor gene P53 in patients with renal cell carcinoma

Author name: اسراء حمزة المعموري

Supervisor name: سيف جبار ياسر

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

دراسة حول فايروس الانفلونزا المعزول من نوبة وبائية في بغداد خلال الفترة 1988/1987 == A Study Of Influenza Virus Isolated From An Outbreak In Baghdad During 1987 - 1988

Author name: علي سالم الدباغ

Supervisor name: نوال الجنابي

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: Mustansiriyah University

Language: English

University location: Baghdad

First pages:

التحري عن فيروسي التورو والغدي عند الاطفال المصابين بالاسهال الحاد في محافظة بابل == Detection Of Torov- and Adeno Viruses in Children Infected With Acute Diarrhea In Babylon Province

Author name: زيتون عبد الرضا اكحيوش الخفاجي

Supervisor name: غانم عبود المولى | هاشم رحيم طارش

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

الدور المحتمل لفايروس EBV في التسبب في سرطان البروستات == The Possible Role of EBV as a cause of Prostate Cancer

Author name: ليلى عبد الكريم وحيد

Supervisor name: باسم شهاب احمد | داود سلمان داود

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Doctorate

University: Mustansiriyah University

Language: English

University location: Baghdad

First pages:

الكشف الجزيئي عن فايروس الجي سي وجينات البي ثلاثة وخمسون والشبكية الجيني والبتا - كتنين في المرضى المصابين بالاورام الغدية للقولون والمستقيم == MOLECULAR DETECTION OF JC VIRUS, P53, RETINOBLASTOMA GENES AND ? - CATENIN IN PATIENT WITH COLORECTAL ADENOCARCINOMA

Author name: ابراهيم فاضل ابراهيم الدروبي

Supervisor name: فائزة عبد الله مخلص | بان عباس عبد المجيد

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Doctorate

University: University of Baghdad - Faculty Of Medicine

Language: English

University location: Baghdad

First pages:

Abstract: تمهيد : يعود فايروس الجي سي الى عائله الفيروسة التوارمية. ينتشر الفايروس بين السكان وعلى نطاق واسع حول العالم. يحمل الفايروس جينة Ag T والتي لها القدره على التكوين السرطاني في الانسجة من خلال التفاعل مع المسارات التنظيمية المختلفه, ويمكن ان تتداخل مع التحكم في دوره الخلية واليات عدم الاستقرار الجيني.العلاقة بين فايروس الجي سي وسرطان القولون وسرطان المستقيم لايزال المنظر ينتضرالقرار النهائي.اهداف الدراسة : 1. تحد يد الدور المحتمل لفايروس الجي سي في سرطان القولون والمستقيم من خلال الكشف والتقدير الكمي لجينة التي وتحميل جينة الاكنوبروتين واظهار الحامض النووي الدي ان اي للفايروس في كل من الخزعات النسيجية السرطانية والطبيعية للقولون والمستقيم. 2. الكشف عن الايجابية المصلية من خلال فحص الاجسام المضادة للفايروس في مصول مجموعه البحث.3. تقدير الرابطة المحتملة بين فايروس الجي سي مع البيتاكاتانين وملاحظة قابلية الفايروس على كبح او تحديد عمل الجينات المشبطة للاورم البي خمسة وثلاثون والشبكية الجيني.طرق العمل : تم تصميم البحث كدراسة مستقبلية للسيطرة على الحالة وانطوت على اخذ الجزعات الطازجة لانسجة القولون والمستقيم من خلال تنظير القولون والمستقيم وقد شملت على 28 مريض مصاب بورم القولون والمستقيم الخبيث وثلاث وثلاثون مريض ممن لم يشخص لديهم ورم سرطاني قي القولون والمستقيم والذين راجعوا وحدة التنظير في مستشفى الاورام ومستشفى بغداد التعليمي ومستشفى اليرموك التعليمي خلال الفتره من حزيران 2013 ولغاية اذار2014. تم الكشف عن جينة فايروس الجي سي ( التي جين) والاكنوبروتين بواسطة جهاز تفاعل سلسلة البلمره وكذلك فحص الحامض النووي الدي ان اي لفايروس الجي سي من خلال التهجين الموقعي وكذلك تم الكشف عن الاجسام المصلية المضادة للفايروس بطريقة الاليزا. تم الكشف عن البيتا - كتنين وبروتين الشبكية الجيني بطريقة المعلمات المناعية والكشف عن البي خمسة وثلاثون بتقنية التهجين الموقعي الفلوري.النتائج• بالنسبة لمجموعة السرطان كان اعلى نسبة تردد للاصابة بورم القولون والمستقيم (35.71%) في المجموعة العمرية مابين 50 - 59 سنة. كان سرطان القولون والمستقيم اكثر شيوعا في الذكور منه في الاناث وبنسبة 1.8 : 1.• اضهر الفحص النسيجي ان النوع الاكثر شيوعا كان بنسبة 85.72% من نوع غدية غير ميوسينية.وان ورم القولون الاكثر شيوعا هو غدية متباينة بشكل معتدل (75%) بين درجات غدية ورم القولون والمستقيم• تم الكشف عن جينة الورم لفايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل وسجل اعلى تردد للاصابة(57.1%) واعلى نسبة نسخ من الجين في مجموعة السرطان مقارنته بمجموعة السيطرة (27.3%) وكانت نتائج الحمل الفايروسي لمجموعة السرطان بمعدل416.93±217.77 نسخة/مايكروغرام اما مجموعة السيطرة فكانت بمعدل229.866±111.49 نسخ /ميكروغرام والتي اضهرت بدورها فرق كبير بين مجموعات الدراسة.• تم تشخيص جينة الاكنوبروتين لفايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل في مجموعة السرطان وكانت النتسبة ( 53.6%) وهي اعلى من النسبة التي تم تشخيصها في مجموعة السيطرة والتي كانت (15.2%). الحمل الفايروسي في مجموعة السرطان كان بمعدل 317±129.12 نسخة/ مايكروغرام وهو اعلى من معدل الحمل الفايروسي في مجموعة السيطرة (152.94±10514) نسخة/ مايكروغرام .• وجد اتفاق كبير بين نتائج تشخيص جينة التي وجينة الاكنوبروتين بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل لمجموعة السرطان ومجموعة السيطرة.• بطريقة التهجين الموقعي لل(دنا) لفيروس الجي سي, اضهرت النتائج ان 53.6% من مجموعة السرطان اضهرو نتائج موجبة لفايروس الجي سي . اما مجموعة السيطرة فاضهرت (30.3%). وكان الفروق ذات دلاله احصائية بين مجموعاتا لدراسة P<0.05 . تبين وجود فروق ذات دلالة احصائية بين مجموعات الدراسة بشان كثافة اشارة ونسبة الاصابة ونمط تورط (P <0.05)، في حين لا توجد فروق كبيرة عثر فيما يتعلق بالمشاركة غدي او اللحمية (P = 0.458)• لم يتم العثورعلاقة ذات دلالة احصائية عند مقارنة ال (دنا ) بواسطة التهجين الموقعي وفقا لدرجات ومواقع الورم.• وجد اتفاق كبير بين نتائج تشخيص جينة التي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج التشخيص بطريقة التهجين الموقعي لل ( دنا) بين مجموعات الدراسة.• وجد اتفاق متوسط بين نتائج تشخيص جينة الاكنوبروتين بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج تشخيص فايروس الجي سي بطريقة التهجين الموقعي لل (دنا) بين مجموعات الدراسة.• فحص المضاد المناعي لفايروس الجي سي بطريقة الاليزا اضهرت وجود مضاد مناعي في 14/ 22(63.6%) مريض من مجموعة السرطان في حين وجد ان 15/28 (53.6%) من مجموعة السيطرة تحمل المضاد المناعي. (p=0.474) وجد توافق ضعيف بين نتائج تشخيص فايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج التهجين الموقعي لل (دنا) ونتائج فحص المضاد المناعي في مجموعات الدراسة.• تم الكشف عن توطين البتا كتنين في النووية بطريقة الفحص النسيجي المناعي الكيمياوي وكانت بنسبة12/28 ( 42.86%) من مجموعة السرطان، في حين لم يكشفت على تلطيخ نووي ايجابي في مجموعة السيطرة (P <0.05). لم يتم العثور على علاقة احصائية لا مع درجات الوروم ولا الى موقع الورم.• تم الكشف عن بروتين جينة الشبكية الجيني بتقنية الفحص النسيجي المناعي الكيمياوي.بالنسبة لمجموعة السرطان 10/28 (35.7%) اضهرت تلطيخ نووي ايجابي في حين ان 18/28 (64.3٪) كشفت عن تلطيخ نووي سلبي اما مجموعة السيطره فاضهرت(100%) تلطيخ ننووي ايجابي. لا اتفاق بين نتائج فحص جين الرتينوبلاستوما وجينة التي وجينة الاكنوبروتين وكذلك التهجين الموقعي لل (دنا) بين مجموعات الدراسة.• بطريقة التهجين الموقعي الفلوري تم الكشف عن التعبير الجيني للبي خمسة وثلاثون. فوجد ان خمسة حالات تم شطب موقع البي خمسة وثلاثون من كرومسوم رقم 17. ثلاث حالات وجدت فيها شطب كروموسوم 17 وحالة واحد وجد فيها خليط من شطب موقع البي خمسة وثلاثون وايضا شطب لكروموسوم رقم 17.الاستنتاجاتابرزت هذه الدراسة الدور المحتمل للفايروس الجي سي التي قد تشارك في طرق مختلفة في التسبب في سرطان القولون والمستقيم، والتي تتفق مع دراسات اخرى. على الرغم من هذه الادلة دور JCV في الاورام الخبيثة القولون والمستقيم وoncoproteins في تعزيز التحول لا يزال بعيدا عن الوضوح. | John Cunningham Virus (JCV) a member Polyomaviridae family is a type of human polyomavirus. It’s widespread virus detected in different populations throughout the world. JCV encodes for T - Ag which have oncogenic capability through interaction with different regulatory pathways and can interfere with cell cycle control and genomic instability mechanisms. The association between JCV and colorectal carcinoma still awaits the final conformation. Aims of study : 1. Determine the possible role of JCV in colorectal carcinoma by detection, quantification of T - Ag gene and agnoprotein gene load and demonstrating JCV DNA in both colorectal carcinoma and normal colonic tissue biopsies. 2. Detect the seropositivity of JCV antibodies among the study groups.3. Estimate the possible interaction of T - Ag with B - catenin and P53 and retinoblastoma tumor suppressor genes. Methods : This study was designed as prospective case - control study that involved fresh colonic tissue biopsies taken through colonoscopy from 28 patients with colorectal cancer and from 33 patients who did not have colorectal carcinoma attending to GIT endoscopic unit of Oncology Hospital, Baghdad Teaching Hospital, and Al - Yarmouk Teaching Hospital during the period from June 2013 to march 2014. JCV DNA was detected and quantified by real time PCR for T - Ag gene and agnoprotein genes. Using JCV bioprobe, chromogenic in situ hybridization (CISH) technique was used to detect JCV in tissue biopsies. Serological detection of JCV IgG antibodies was detected by ELISA. B - catenin and retinoblastoma protein products were detected by immunohistochemistry. Tumor suppressor gene P53 was detected by fluorescent in situ hybridization technique. Results : • Among the total 28 colorectal carcinoma cases, the highest frequency 10/28(35.7%) were detected in age group 50 - 59 years and its more frequent in male than female with ratio 1.8 : 1.• Histopathological finding revealed that 24(85.72%) cases were non mucinous adenocarcinoma and moderately differentiated adenocarcinoma was more frequent 21/28 (75%) among grades of CRC.• JCV T - Ag was detected in 16 (57.1%) of CRC group and 9/33(27.3%) of control group, with viral load VL mean 416.93 ± 217.77 copy/µg for CRC group and 229.866 ± 111.49 copy/µg for the control group which in turn showed significant difference between the study groups• Agnoprotein gene was detected in 12/28 (42.9%) of CRC group with VL mean (317 ± 129.12) compared to 5/33 (15.2%) with VL mean (152.94 ± 105.14) in non CRC patient (p=0.016). • Substantial agreement was found between T Ag gene and agnoprotein gene by real time PCR for both CRC group K=0.72 and control group K=0.784.• Using chromogenic in situ hybridization technique JCV DNA was detected in 15/28(53.6%) of CRC compared to 10/33(30.3%) in control group (p=0.065). Significant differences were detected comparing the percentage of JCV DNA in study groups according to intensity, score and signal pattern of involvement (p<0.05).• Substantial agreement was found between T Ag by qPCR and JCV DNA by CISH among the study groups. Moderate agreement was found between JCV agnoprotein gene by qPCR and JCV DNA by CISH among the study groups. • Serological detection of JCV antibodies by ELISA revealed 14/22 (63.6%), 15/28 (53.6%) of CRC group and control group showed positive results respectively. (p=0.474). Poor agreement was found between (T Ag by real time PCR and by CISH) and JCV antibodies by ELISA in the study groups.• Nuclear localization of B - catenin was detected by immunohistochemistry in 12/28(42.86%) of CRC groups, while none of the control group revealed positive nuclear staining (p<0.05). No significant associations found neither to grades nor to the site of tumor.• Retinoblastoma protein was detected by IHC. Only ten out of 28 (35.7%) showed positive nuclear staining, 18/28 (64.3%) of CRC revealed negative nuclear staining. All control group showed positive nuclear staining (p=0.000). No agreement was found between Rb protein and JCV T Ag, agnoprotein gene and JCV DNA by CISH among study groups.• P53 deletion was detected using FISH technique. Five cases out of 25 were with deleted p53 region, 3/25 were with deleted whole chromosome 17, and 1/25 revealed combination signals of deleted p53 region and deleted whole chromosome 17. The results were invalid for statistical analysis.Conclusion : This study highlighted the possible role of JCV which might participate in different ways in the pathogenesis of colorectal carcinoma, which in agreement with other studies. Despite these evidences the role of JCV in colorectal malignancies and its oncoproteins in promoting transformation is still far from clear

دراسة خمج فيروس الكبد نمط جي HGV في مرضى الثلاسيميا المصابين وغير المصابين بفيروس الكبد سي (HCV) واهميته السريرية

Author name: مريم صبري ابراهيم

Supervisor name: اروى هادي الحمداني | اشنا جمال فائق

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: Mustansiriyah University

Language: English

University location: Baghdad

First pages:

Abstract: المرضى المصابين بفقر دم البحر الابيض المتوسط هم اكثر عرضة للاصابة بالالتهابات الفيروسية المنقولة دمويا. التهاب الكبد الفيروسي نمط جي (HGV)هو فيروس رنوي(RNA) مرتبط بالتهاب الكبد الفيروسي الحاد والمزمن. التهاب الكبد الفيروسي نمط سيHCV) ) والتهاب الكبد الفير | Thalassemia patients are at increased risk of infection with parentally transmitted viral agents. The hepatitis G virus (HGV) is an RNA virus, which is associated with acute or chronic hepatitis. Hepatitis G virus (HGV) and hepatitis C virus (HCV) infections may have a role in complicating the clinical outcome in patients with thalassemia. This study was designed to investigate the prevalence and clinical importance of HGV infection in thalassemia patients with or without hepatitis C virus (HCV) co - infection, furthermore to sequence and analyze phylogentic of HGV clones. One hundred fifty four thalassemia patients (56.5% male, 43.5% female) with a mean age of 22.84±6.06 years were involved in this study that was conducted in the period between Feb. to May, 2014. Anti - HCV antibody was determined by Enzyme Linked Immunosorbent Assay (ELISA) and confirmed by western blot. Then, HCV - RNA was detected in 53.2% of anti - HCV positive thalassemia patients. HGV antibodies were evaluated by ELISA. Also, the HGV viremia was analyzed in patients with thalassemia by reverse transcription polymerase chain reaction (RT - PCR) protocol. Results obtained showed that the anti - E2 - HGV were found in 16 out of 154 (10.4%) thalassemia patients with significant decrease in its prevalence with increasing age, while HGV viremia was diagnosed in 28 out of 154 (18.2%) patients with thalassemia. No association of HGV infection was found with gender, age and frequency of blood transfusion.According to HCV and HGV infection status, thalassemia patients were categorized into four subgroups : subgroup I (HGV infection), subgroup II (HCV infection), subgroup III (co - infection of HCV and HGV), and subgroup IV (thalassemia patients with neither HCV nor HGV infection). Seven point one percent (7.1%) of thalassemia patients were found to be co - infected with HCV and HGV.In all thalassemia subgroups, liver transaminases; alanine transaminase (ALT) and aspartate transaminase (AST), alkaline phosphatase (ALP), and total serum bilirubin (TSB) were determined, in addition to measure serum ferritin levels by VIDAS. HCV infection shows significant increase in ALT level while in HGV is not. Co - infection with HCV and HGV decrease ALT levels when compared with infection with HCV alone, so HGV infection is suggested to have no role in increasing the severity of liver diseases in the thalassemia patients. In HGV infection, there were significant increase in ALP levels and significant decrease in ferritin levels than other subgroups in the study. However, HGV infection shows no significant differences in AST and TSB than other subgroups. The results of genotyping in 12 randomly selected patients showed presence of genotype 2 and genotype 5 with percentage of 91.7% and 8.3% respectively. The diagnosis of prevalence of HGV and HCV in patients with thalassemia in Iraq emphasized the importance of these lymphotropic viral hepatitis infections in pathogenesis and outcome of thalassemia patients.

دراسة جزيئية للاصابات بفايروس الحليمي البشري وفايروس سرطان الغدد اللبنية البشري في المريضات المجراة لهن عملية استئصال الرحم الكامل == Molecular Study Of Human Papilloma Virus And Human Mammary Tumor Virus Infections Of Patients With Total Hysterectomy

Author name: سري ظافر داود العزيز

Supervisor name: سعد حسن محمد علي | بسام شهاب احمد

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Doctorate

University: Mustansiriyah University

Language: English

University location: Baghdad

First pages:

Abstract: Theoretically , the origin & mode of in - utero transmission of high risk Human PapillomaVirus (HR - HPV) types could be acquired hematogenously, by semen at fertilization or as asending infection in the genital tract. Viral Oncogenes E6 and E7 have ability to altered the Cyclin D1,p21 and p27 protein levels. Worldwide, Human Mammary Tumor Virus(HMTV) infection been described in hormonal responsive tissues.This research work is to study viruses in relaition to endometrial carcinoma in Iraqi patients.Materials and MethodsA retrospective study envolved seventy(70) cases (158 samples) of endometrial and cervical tissues collected from teaching laboratories in Medical City, Al - Yarmok Teaching Hospital and private laboratories related to the period from 2012 to 2014. Sections were made and subjected to chromogenic in - situ hybridization technique to detect the presence of High risk (HR) HPV 16/18 DNA and HMTV integrated DNA (provirus) and immunohistochemical technique to detect the presence of Cyclin D1,P21and P27.Results : The most commonly affected age group was ? 60 year 60.0% in malignant endometrial tumors, 40 - 49 years 36.0% in non - malignant endometrial tumors. Adenocarcinoma was the only histopathological type among endometrial cancer (100%) and the majority have well differentiated grade (90.0% ),and have (70%) T1b/IB stage.Hight risk HPV16/18 expression was detected 33.3% of malignant endometrial tumors, 20% in non - malignant endometrial tumors, andSUMMARY26.7% in control group. The higher rates of expression of HPV in cervical lesions was 40%.Most HPV16/18 infections revealed in both sites (cervix and endomertium) have Punctate DNA pattern.Cyclin D1 expression was mostly in 43.3% of malignant endometrial tumors and 33.3% was in cervical lesions. The significant correlation between Cyclin D1 expression and HR - HPV16/18 infection was reported only in malignant endometrial tumors groups. The highest P21 expression was 40.0% in malignant endometrial tumors and 44.0% in cervical lesions that reveals a significant association between p21 expression and HR - HPV16/18 infection. The highest rates of P27 expression were noticed among non - malignant endometrial tumors 24.0% and 32.0% in cervical lesions.While significant association of P27 expression with HR - HPV16/18 infection was only found in the cervical lesions among malignant endometrial tumors group.The distribution of integrated HMTV genome detected by Chromogenic in - situ hybridaztion (CISH) in the endometrial tissues was 16.7% in malignant endometrial tumor , 8% in non - malignant endometrial tumors, and 13.3% in the control tissues group while in the cervical lesions HMTV was detected in 13.3%. Significant correlations were found between HMTV infection and Cyclin D1 expression in the cervical lesions among malignant endometrial tumors group and p21 expression in the malignant endometrial tumors.ConclusionsHR - HPV16/18 may be associated with initiation of endometrial carcinogenesis events as well as play a role in the progression of such malignant tumors in addition ,these viruses could ascendingly be transmitted from lower part(cervix) to the upper part of the genital tractSUMMARY(endometrium) in some way or other.Cyclin D1 ,P21,and p27 could have sharing in early events of tumorgenesis in endometrial carcinoma and a significant correlations of Cyclin D1 ,p21& p27 expressions with HPV infection was observed among study group.Althought a relating low rates of HMTV infection were detected among different endometrial as well as cervical lesion in this Iraqi study,it might mark or shade alight in the way for further researches in this field to exposure more aspects of this newly studied virus among other hormonal dependent malignancy

دراسة التنميط الجيني لفيروس الSEN في متبرعي الدم الاصحاء ومرضى الثلاسيميا المصابين وغير المصابين بفيروس التهاب الكبد نمط ج واهميته السريرية == Genotypic Study Of SEN Virus In Healthy Blood Donors And Thalassemia Patients; With Or Without HCV Infection And Its Clinical Importance

Author name: بشار منذر خزعل الزبيدي

Supervisor name: اروى هادي الحمداني | اشنا جمال فائق

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: Mustansiriyah University

Language: English

University location: Baghdad

First pages:

Abstract: Blood transfusion is one of the most common routes of viral hepatitis transmission among population. There are several diseases need continuous blood transfusions to manage the patient's condition; one of these diseases is ?thalassemia, so these patients are at increased risk of infection with blood transfusion transmitted agents. SEN - Virus is a DNA virus which is associated with acute post - transfusion hepatitis and the prevalence with the clinical importance and also the genetic characterization are still much unknown in Iraq; therefore, this study was designed to investigate the occurrence and the clinical importance of SEN - virus infection in healthy blood donors and thalassemia patients with or without HCV infection and then the study of the genome sequencing and the phylogenetic analysis of SEN - V clones. One hundred and fifty eight thalassemia patients (57.6% male, 42.4% female), with mean age of 16.8±8.5 year, and one hundred and fifty healthy blood donors with randomly selected persons (58.7%male, 41.3% female), with mean age of 16.7±8.6 year. All these samples involved in this study that were conducted in the period between January to June 2015. SEN - V had been identified by DNA extraction, DNA amplification by nested conventional PCR and then Agarose gel electrophoresis amplified DNA bands detection; while HCV was identified by RNA extraction, RNA reverse transcription into complementary DNA, DNA amplification by nested conventional PCR and then Agarose gel electrophoresis amplified DNA bands detection. Liver transaminases (Aspartate Transaminase and Alanine Transaminase) were determined, in addition of measure of serum ferritin levels by VIDAS. SEN - V was detected in 68 out of 158 (43%) thalassemia patients and 16 out of 150 (10.7%) blood donors. HCV prevalence was (11.4%) in thalassemia patients. There was significant increase observed in the occurrence of SEN - V or HCV infection with age but there was no significant difference observed in the occurrence of both with gender. The most important (hepatotropic) SEN - V genotypes were SEN - V - D and SEN - V - H and there were patients infected with D genotype or H genotype alone, while there were patients co - infected with D and H. According to HCV and SEN - V co - infection status, patients and controls subdivided into six subgroups : subgroup I was thalassemia HCV RNA + and SENV DNA +, subgroup II was thalassemia HCV RNA + and SEN - V - , subgroup III was thalassemia HCV RNA - and SEN - V DNA +, subgroup IV was thalassemia HCV RNA - and SEN - V - , subgroup V was control SEN - V + and finally subgroup VI was control SEN - V - . Statistical analysis revealed that there was significant increase in AST and ALT levels in subgroup I when compared with the other subgroups - that showed the increasing relationship in the case of infection with both viruses - and there were no any significant differences in ferritin levels among these subgroups. Moreover, there were no significant differences between SEN - V - D, SEN - V - H and SEN - V - D and H co - infected samples in AST, ALT and ferritin among thalassemia patients and controls. The results from the study of gene sequencing and phylogenetic analysis of samples of amplified SEN - V - D and samples of amplified SEN - V - H DNA which were selected randomly from blood donors and thalassemia patients infected with D or H genotypes alone or together (co - infection), revealed that the most transmission route of SEN - V D and H was blood transfusion that is because there was (99%) gene similarity between blood donors and thalassemia patients, furthermore SEN - V - D or SEN - V - H sequences of the co - infected persons were the same sequences of D or H genotypes alone and with the observations of similarity with neighboring countries.

دراسة التنميط الجيني لفيروس تي تي في مرضى الثلاسيميا المصابين وغير المصابين بفيروس التهاب الكبد نمط ج

Author name: ابتسام نايف عودة الجبالي

Supervisor name: اروى هادي الحمداني

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: Mustansiriyah University

Language: English

University location: Baghdad

First pages:

Abstract: ما زال الكثيرون لا يعرفون الاهمية السريرية لفيروس تي تي الذى رشح كاحد فايروسات التهاب الكبد انماط A - G)).ان فيروس تي تي يصيب المرضى المعرضين لخطر الحقن ونقل الدم المتكرر، كما في المرضى المصابين بفقر دم البحر الابيض المتوسط(? - Thalassemia major). تمت هذة | Much is still unknown about the clinical significance of TT virus (TTV) that has been reported as a candidate for non A - G hepatitis virus. TTV infects patients at risk for parenteral exposure and chronic blood transfusion, such as those with ? - thalassemia major. This study was designed to investigate the prevalence and clinical importance of TTV infection in thalassemia patients with or without hepatitis C virus (HCV) co - infection, furthermore to sequence and analyse phylogentic of TTV clones.One hundred fifty four thalassemia patients (64.3% male, 35.7% female) with a mean age of 23.8±6.8 years were involved in this study that was conducted in the period between Feb. to Dec. 2013. TTV DNA was detected using Real time PCR. Furthermore, conventional PCR was done for sequencing and phylogenetic analysis using N22 region from open reading frame 1 (ORF1). Anti - HCV antibody was determined by Enzyme Linked Immunosorbent Assay (ELISA) and confirmed by western blot. Then, HCV core antigen was detected in those anti - HCV Abs seropositive samples. Liver transaminases (ALT, AST) were determined, in addition to measure serum ferritin levels by VIDAS ferritin. TTV was detected in 45 of 154 (29.2%) of thalassemia patients with predominance of males than females (64.4% vs. 35.6%). The prevalence rate of anti - HCV Abs were 54.5% with significant increase in females than males (p<0.05).Out of these 84 samples, 59 (70.2%) were positive by HCV core Ag assay and related significantly to frequency of blood transfusion. According to HCV and TTV infection status, patients were categorized into six groups : Anti - HCVAb+ HCV core Ag + TTV+, Anti - HCVAb+ HCV core Ag + TTV - , Anti - HCVAb+ HCV core Ag - TTV+, Anti - HCVAb+ HCV core - TTV - , Anti - HCVAb - TTV+, Anti - HCVAb - TTV - . The increased levels of ALT, AST and ferritin in the HCV and TTV infected group were not significantly different from those in the TTV and HCV negative groups. Co - infection of TTV and HCV were significantly increase ALT levels compared to infection with HCV alone. No association of TTV infection was found with gender, age and frequency of blood transfusion.The results of genotyping in 12 randomly selected patients showed the presence of equal percentage of genotype 1 (G1) and genotype 2 (G2) 50% for each one, with homology between them 79.0%. Other TTV genotypes were not identified in the present study suggesting that G1and G2 are predominant in this area. Mixed infection of the same patient with multiple TTV genotypes was observed. The results concluded that TTV is moderately present in Iraqi thalassemia patients, with G1 and G2 were predominant. Patients co - infected with HCV and TTV had a significantly higher serum ALT level than those without TTV infection, so TTV infection is suggested to have a role in increasing the severity of liver diseases in the thalassemia patients when co - infected with HCV.

دراسة التركيب الوراثي الجزيئي والمناعي على مرضى الذين يعانون من فيسروس التهاب الكبد نمط B في الفرات الاوسط من العراق == Molecular Genotypes And Immunological Study On Patients With Hepatitis B Virus In Middle Euphrates of Iraq

Author name: عباس عطية شعلان سراق

Supervisor name: غانم عبود جابر المولى | سلمان عزيز عدوس

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

التحري عن الفايروس البشري المضخم للخلايا في حالات الاجهاض التلقائي والعقم == Detection Of Human Cytomegalovirus Infections In Spontaneous Miscarriage And Infertility

Author name: سيف جبار ياسر

Supervisor name: كريم ثامر مشكور الكعبي | غانم عبود المولى

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

مسح شامل للاجسام المضادة الفايروسية في مرض داء السكر

Author name: داود سلمان وهبي

General topic: Medicine

Specific topic: Microbiology - Viruses

Degree: Master

University: University of Baghdad - Faculty Of Medicine - Department Of Microbiology

University location: Baghdad