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دراسة التنميط الجيني لفيروس الSEN في متبرعي الدم الاصحاء ومرضى الثلاسيميا المصابين وغير المصابين بفيروس التهاب الكبد نمط ج واهميته السريرية == Genotypic Study Of SEN Virus In Healthy Blood Donors And Thalassemia Patients; With Or Without HCV Infection And Its Clinical Importance
Author name:
بشار منذر خزعل الزبيدي
Supervisor name:
اروى هادي الحمداني | اشنا جمال فائق
General topic:
Medicine
Specific topic:
Microbiology - Viruses
Degree:
Master
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1134 - p.pdf
Abstract:
Blood transfusion is one of the most common routes of viral hepatitis transmission among population. There are several diseases need continuous blood transfusions to manage the patient's condition; one of these diseases is ?thalassemia, so these patients are at increased risk of infection with blood transfusion transmitted agents. SEN - Virus is a DNA virus which is associated with acute post - transfusion hepatitis and the prevalence with the clinical importance and also the genetic characterization are still much unknown in Iraq; therefore, this study was designed to investigate the occurrence and the clinical importance of SEN - virus infection in healthy blood donors and thalassemia patients with or without HCV infection and then the study of the genome sequencing and the phylogenetic analysis of SEN - V clones. One hundred and fifty eight thalassemia patients (57.6% male, 42.4% female), with mean age of 16.8±8.5 year, and one hundred and fifty healthy blood donors with randomly selected persons (58.7%male, 41.3% female), with mean age of 16.7±8.6 year. All these samples involved in this study that were conducted in the period between January to June 2015. SEN - V had been identified by DNA extraction, DNA amplification by nested conventional PCR and then Agarose gel electrophoresis amplified DNA bands detection; while HCV was identified by RNA extraction, RNA reverse transcription into complementary DNA, DNA amplification by nested conventional PCR and then Agarose gel electrophoresis amplified DNA bands detection. Liver transaminases (Aspartate Transaminase and Alanine Transaminase) were determined, in addition of measure of serum ferritin levels by VIDAS. SEN - V was detected in 68 out of 158 (43%) thalassemia patients and 16 out of 150 (10.7%) blood donors. HCV prevalence was (11.4%) in thalassemia patients. There was significant increase observed in the occurrence of SEN - V or HCV infection with age but there was no significant difference observed in the occurrence of both with gender. The most important (hepatotropic) SEN - V genotypes were SEN - V - D and SEN - V - H and there were patients infected with D genotype or H genotype alone, while there were patients co - infected with D and H. According to HCV and SEN - V co - infection status, patients and controls subdivided into six subgroups : subgroup I was thalassemia HCV RNA + and SENV DNA +, subgroup II was thalassemia HCV RNA + and SEN - V - , subgroup III was thalassemia HCV RNA - and SEN - V DNA +, subgroup IV was thalassemia HCV RNA - and SEN - V - , subgroup V was control SEN - V + and finally subgroup VI was control SEN - V - . Statistical analysis revealed that there was significant increase in AST and ALT levels in subgroup I when compared with the other subgroups - that showed the increasing relationship in the case of infection with both viruses - and there were no any significant differences in ferritin levels among these subgroups. Moreover, there were no significant differences between SEN - V - D, SEN - V - H and SEN - V - D and H co - infected samples in AST, ALT and ferritin among thalassemia patients and controls. The results from the study of gene sequencing and phylogenetic analysis of samples of amplified SEN - V - D and samples of amplified SEN - V - H DNA which were selected randomly from blood donors and thalassemia patients infected with D or H genotypes alone or together (co - infection), revealed that the most transmission route of SEN - V D and H was blood transfusion that is because there was (99%) gene similarity between blood donors and thalassemia patients, furthermore SEN - V - D or SEN - V - H sequences of the co - infected persons were the same sequences of D or H genotypes alone and with the observations of similarity with neighboring countries.
