دراسة جزيئية للاصابات بفايروس الحليمي البشري وفايروس سرطان الغدد اللبنية البشري في المريضات المجراة لهن عملية استئصال الرحم الكامل == Molecular Study Of Human Papilloma Virus And Human Mammary Tumor Virus Infections Of Patients With Total Hysterectomy
Author name:
سري ظافر داود العزيز
Supervisor name:
سعد حسن محمد علي | بسام شهاب احمد
General topic:
Medicine
Specific topic:
Microbiology - Viruses
Degree:
Doctorate
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1139 - p.pdf
Abstract:
Theoretically , the origin & mode of in - utero transmission of high risk Human PapillomaVirus (HR - HPV) types could be acquired hematogenously, by semen at fertilization or as asending infection in the genital tract. Viral Oncogenes E6 and E7 have ability to altered the Cyclin D1,p21 and p27 protein levels. Worldwide, Human Mammary Tumor Virus(HMTV) infection been described in hormonal responsive tissues.This research work is to study viruses in relaition to endometrial carcinoma in Iraqi patients.Materials and MethodsA retrospective study envolved seventy(70) cases (158 samples) of endometrial and cervical tissues collected from teaching laboratories in Medical City, Al - Yarmok Teaching Hospital and private laboratories related to the period from 2012 to 2014. Sections were made and subjected to chromogenic in - situ hybridization technique to detect the presence of High risk (HR) HPV 16/18 DNA and HMTV integrated DNA (provirus) and immunohistochemical technique to detect the presence of Cyclin D1,P21and P27.Results : The most commonly affected age group was ? 60 year 60.0% in malignant endometrial tumors, 40 - 49 years 36.0% in non - malignant endometrial tumors. Adenocarcinoma was the only histopathological type among endometrial cancer (100%) and the majority have well differentiated grade (90.0% ),and have (70%) T1b/IB stage.Hight risk HPV16/18 expression was detected 33.3% of malignant endometrial tumors, 20% in non - malignant endometrial tumors, andSUMMARY26.7% in control group. The higher rates of expression of HPV in cervical lesions was 40%.Most HPV16/18 infections revealed in both sites (cervix and endomertium) have Punctate DNA pattern.Cyclin D1 expression was mostly in 43.3% of malignant endometrial tumors and 33.3% was in cervical lesions. The significant correlation between Cyclin D1 expression and HR - HPV16/18 infection was reported only in malignant endometrial tumors groups. The highest P21 expression was 40.0% in malignant endometrial tumors and 44.0% in cervical lesions that reveals a significant association between p21 expression and HR - HPV16/18 infection. The highest rates of P27 expression were noticed among non - malignant endometrial tumors 24.0% and 32.0% in cervical lesions.While significant association of P27 expression with HR - HPV16/18 infection was only found in the cervical lesions among malignant endometrial tumors group.The distribution of integrated HMTV genome detected by Chromogenic in - situ hybridaztion (CISH) in the endometrial tissues was 16.7% in malignant endometrial tumor , 8% in non - malignant endometrial tumors, and 13.3% in the control tissues group while in the cervical lesions HMTV was detected in 13.3%. Significant correlations were found between HMTV infection and Cyclin D1 expression in the cervical lesions among malignant endometrial tumors group and p21 expression in the malignant endometrial tumors.ConclusionsHR - HPV16/18 may be associated with initiation of endometrial carcinogenesis events as well as play a role in the progression of such malignant tumors in addition ,these viruses could ascendingly be transmitted from lower part(cervix) to the upper part of the genital tractSUMMARY(endometrium) in some way or other.Cyclin D1 ,P21,and p27 could have sharing in early events of tumorgenesis in endometrial carcinoma and a significant correlations of Cyclin D1 ,p21& p27 expressions with HPV infection was observed among study group.Althought a relating low rates of HMTV infection were detected among different endometrial as well as cervical lesion in this Iraqi study,it might mark or shade alight in the way for further researches in this field to exposure more aspects of this newly studied virus among other hormonal dependent malignancy
