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التقييم المناعي للقاحات المحضرة من العزلة المحلية للمكورات العنقودية المقاومة للميثيسيلين == Immunological Evaluation of Vaccines Prepared from Local Isolate Methicillin Resistant Staphylococcus aureus

Author name: عبد الله عبد العزيز خليل شيحان
Supervisor name: محمد حسن خضر | فنار ابلحد اسحق
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Basrah
Key words:
  • الاحياء المجهرية - المناعة
First pages:

الكشف عن انواع الايميريا في الاغنام والماعز في بعض مناطق محافظة البصرة جنوب العراق == Detection of Eimeria spp. in Sheep and Goats in some areas of Basrah Province, Southern Iraq

Author name: هناء عبد الحسين شهيد
Supervisor name: سوزان عبد الجبار عبد العزيز
General topic: Veterinary Medicine
Specific topic: Parasites
Degree: Master
Language: English
University location: Basrah
First pages:

دراسة تشخيصية وامراضية نسجية للقناة الهضمية في الدجاج المنزلي Gallus gallus domasticus المصابة بالطفيليات المعوية في مدينة البصرة == Diagnostic And Histopathological Study of Intestinal Tract of Domestic Fowl Gallus Gallus Domesticus Infected With Intestinal Parasites At Basrah City

Author name: اسراء صبيح بداي الغنامي
Supervisor name: غازي يعقوب عزال الامارة
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: امتدت الدراسة الحالية من شهر تشرين الاول 2011 ولغاية شهر اذار 2012 , حيث جمعت خلالها 108 نموذجا بواقع 36 عينة من الذكور و72 عينة من الاناث من الدجاج المحلي1758) Gallus gallus domesticus (Linnaeus, من محافظة البصرة بهدف عزل الديدان المتطفلة على القناة الهض | The recent study extended from October 2011 to January 2012, collected 108 samples by 36 samples of males and 72 samples of female of local chickens Gallus gallus domesticus (Linnaeus, 1758)from Basrah to isolate parasitic worms on the gastrointestinal tract and diagnosed as well as study percentage and severity of the infection, as well as monitoring biological activities of isolated parasites and find out macroscopic and pathological changes caused by these worms and find out the impact of tapeworms on the values of the blood parameters of chicken. The results showed infected of chicken with five types of tapeworms (Hymenolepis carioca, Raillietina tetragon, Raillietina cesticillus, Choanotania infundibulum, Cotugina sp) with percent of infection and severity reached 4.62%, 11.4; 19.44%, 12.09; 5.55%, 12.66; 9.25%, 12.6; 6.48 %, 11.14 respectively and got one type of nematodes Subulura brumpti with percentage of infection and severity reached 10.18%, 91 and three types of trematode Echinostoma revulatum, Hypoderm coniodium, Psilolecithum longorchum with percent of infection and severity reached 1.85%, 15.5; 0.92%, 10; 0.92% , 7 respectively.The results of the recent study showed that infection with two types is the most common reaching incidence of 25.92%, and study results indicated that there were significant differences between males and females in susceptibility to intestinal worms.Observed by studying the sites of worms in the gut that the proportion of tapeworms in the small intestine are more than in the large intestine while recorded nematodes only in the large intestine As for worms Trematode has found in the small intestine. The results also included monitoring biological activities of isolated parasites from the gastrointestinal tract from the beginning of the division of the egg and develop into a larva has been observed that the nematodes have the ability to survive outside the host, who were present more than tapeworms.the study contained a physiologic side on the impact of tapeworms on some blood parameters where showed infection of domestic fowl with anemia by observing the decrease in the number of red blood cells R.BC , hemoglobin Hb and P. CV while there was an increase in the number of white blood cells WBC. The study recorded the number of grossly and histological lesions in the intestine and the cecum of domestic chicken Because they infected with tapeworms, nematodes and flukes. grossly lesions included blockage and swelling in the intestines infected with tapeworm and cecum infected with nematode and appearance of rough nodules on the walls of the intestine infected with tapeworm with hemorrhage and the appearance of yellowish green purulent material in the inner wall of the intestines, and the histological changes has included atrophy of intestinal villi with sloughing and desquamation of epithelial layer with appearance of inflammation in the form of pseudomembrane and infiltration of inflammatory cells and coaglative necrosis, it have seen hemorrhage and superficial erosion in the mucous layer and sloughing of villi and epithelial layer with hyperplasia appearance purulent cells and destruction of intestinal glands and ulceration in the mucous layer in addition to the congestion

الكشف السيرولوجي والجزيئي لفايروس مرض الحمى القلاعية في الابقار في محافظة البصرة == Serological And Molecular Detection of Fmdv In Cow of Basrah Province

Author name: زينب مجيد سالم الكلي
Supervisor name: عدنان موسى الروضان
General topic: Veterinary Medicine
Degree: Master
Language: English
University location: Basrah
First pages:
Abstract: اجريت هذه الدراسة في6 مناطق مختلفة ( شط العرب والزبير والقرنه وابي الخصيب ومركز البصره والمدينه) ممثله لجميع اجزاء محافظة البصره خلال الفتره من شهر تشرين الاول من العام 2012 الى شهر تموز من عام 2013 ولتحديد نسبة انتشار مرض الحمى القلاعيه في الابقار استخد | This study was conducted on local cattle from 6 different regions (Shutt - Alarab,Al - Zubair, Al - Qurna, Abi - Elkhasib, Basrah center and Almdaina) covering all parts of the Basrah province during the period from October 2012 to July 2013. In order to determine the prevalence of foot and mouth disease in bovine species Enzyme Linked Immune Sorbent Assay ( ELISA) and reverse transcriptase polymerase chain reaction techniques( RT - PCR) were used in the testing of serum ,saliva and vesicular fluid samples collected from clinically diseased cattle, moreover this study was also mentioned some clinical aspects of the disease.Diseased animals showed the following clinical signs : depression(92.1%),sever salivation(90.9%) ,vesicles in mouth(90.3%), fever(89.1%) , conjunctivitis(87.9%) and smacking of lips in higher rates( 80.6%).While the vesicles on teat(12.7%), lameness(36.9%)and vesicles on feet (45.5%)appeared in lower rates.The overall prevalence of this disease was(72.7%.).Of 165 serum samples subjected to nonstructural protein (NSP) ELISA screening test (ELISA CHEKIT FMD - 3ABC Bo - Ov ELISA Test ) , 120 were interpreted as positive representing (72.7%.) while the other 45samples were negative representing 27.3%.The seropositivity significantly associated with age groups (p<0.01) and sex (P < 0.01).Depending on sex of these animals , the higher seropositivity rate was observed in females (78.6%).Concerning the age of tested cattle the animals of 2nd age group (>4 - 8 years) appeared in higher rate (84.8 %) of seropositivity followed by the rate (51.7% ) of 1st age group animals(<1 - 4 years).The sero - positivity against FMDV was non significantly different(>P 0.05)among the cattle in Basrah districts and highest sero - prevalence rate was observed in cattle of Basrah center (100%), Al - Mdaina (77.8%), Shut - Alarab(76.3%) and Abi - Elkhasib (70%).The lower sero - positivity rate was observed in Qurna(58.8%)and Zubair (50%) cattle.RT - PCR detection of FMDV for primary and serotype specific diagnosis was used. of eighty three clinically positive samples including, mouth vesicles, saliva and serum samples tested by RT - PCR, only 68(81.9%) were successfully amplified, their identification was done with universal primer sets (1F / 1R, )with expected band of( 328 bp).Of sixty eight universal primer based RT - PCR positive samples tested for serotype A - 1C562(865bp), O - 1C272( 635bp ) and Asia1 - 1C505(911bp) serotypes primers, 19(27.9%) samples were found to be serotype A - 1C562 positive and 26(38.2%) were serotype O - 1C272 positive and nil for Asia1 - 1C505 serotype. The frequency of foot - and - mouth disease viral genome presence in cattle had significant differences concerning the sex (P<0.05) while the difference among age groups was highly significant (P < 0.01) in case of universal gene and not significant(P>0.05) in case of all serotypes genes. Depending on the sex effect, the rate of RT - PCR positive results of universal gene and O - 1C272 serotype gene were higher in females (87.5 and38.8% respectively ),while A - 1C562 serotype RT - PCR positivity rate was higher in males (31.6% ).Different rates of RT - PCR positivity was observed in the two age groups of cattle and the second age group(>4 - 8 year) showed higher rate of positivity as a follow : (universal primer (90.7%),O - 1C272 serotype primer( 40.8%) and A - 1C562 serotype primer(34.7% ).In the studied regions of Basrah province , the FMDV genes were significantly(P<0.05)distributed. The rates of universal gene appearance were100, 93.3 and 83.3%of cattle in Zubair ,Qurna and Shutt - Alarab respectively. The serotype A - 1C562 was distributed in higher rate ( 100 and 40%) of Zubair and Al - Mdaina cattle respectively , while the serotype O - 1C272 was observed in higher distribution rate in cattle of Abi - Elkhasib (44.4%) and Qurna(42.9%), so there is significant differences ((P<0.05) according to distribution of serotypes of FMDV of regions of Basrah province.

التحري عن داء المقوسات في النساء والنعاج بواسطة طرق مصلية مختلفة في محافظة ميسان - العراق == An Investigation of Toxoplasmosis In Women And Ewes By Different Serological Assays In Maysan Governorate, Iraq

Author name: مرتضى شهاب جبار
Supervisor name: منى محمد جوري
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Basrah
First pages:
Abstract: تم جمع 291 عينة من الدم الوريدي من النساء في سبعة مناطق مختلفة في محافظة ميسان للفترة من تشرين الثاني 2012 وحتى اذار 2013 للكشف عن داء المقوسات باستخدام ثلاثة اختبارات هي اختبار التلازن الحبيبي وانزيم الادمصاص المناعي واختبار المنفايدس ثلاثة مجاميع من الن | In this study, venous blood sample were collected from 291 women in seven different regions of Maysan province during the period from October 2012 to March 2013 for detecting the toxoplasmosis by using three tests (Latex agglutination (LAT), enzyme linked immunosorbent (ELISA - IgG) and minividas. Three groups of women ( pregnant ,non - pregnant and aborted ) and 283 ewes which represent 18 herds from nine regions of Maysan province were selected for the study in order to diagnose the toxoplasmosis by using ELISA and LAT tests. The results showed that the ratio of Toxoplasma seropositivity in aborted women was significantly the highest (69.23%) followed by pregnant group (60.16%) by using LAT. Moreover, the ratio of seropositivity was highest in aborted women (47.43%) followed by pregnant (36.58%) and non - pregnant (26.66%) groups, respectively by using ELISA test. With Minividas assay, the results showed the highest seropositivity percentage in aborted women (26.92%) followed by the pregnant (17.88%) and non - pregnant (11.11%) groups, respectively. According to regions of the study, there was higher seropositivity against T. gondii in women in Al - Musharah subdistrict by LAT (68.09%) and ELISA (44.68%) test, respectively. However, the seropositivity by using minividas assay in three regions in AL - Majar district, Al - Musharah subdistrict and AL - Maymona district were 23.52% , 23.40% , 23.52% , respectively in comparison with other regions in Maysan governorate. The significantly high (P? 0.05) seropositivity detected by LAT (72.63%), ELISA (50.52%) and minividas (28.42%) were in women of age group > 30 years. The results showed that the ratios of Toxoplasma seropositivity in aborted ewes group was the highest (73.33%) followed by pregnant group (65.38%) after using the LAT, while the seropositivity in aborted ewes detected by the ELISA test was 40%. The results from Al - Musharah subdistrict that showed highest seropositivity against Toxoplasma by using LAT and ELISA tests were 78.26% and 39.13%, respectively. The highest Toxoplasma seropositivity in the two - years age - group ewes was 65.57% and one - year age - group was 65.30% after using LAT test, while was 32.65% in the age - group more than a year by ELISA. The statistical analysis showed significant difference (p ? 0.05) between the LAT and ELFA tests conducted for women samples, but there were no significant differences (p? 0.05) between LAT and ELISA carried out for women and ewes samples with regarded to Toxoplasma seropositivity.

تشخيص وتوصيف جراثيم الاشريشيا القولونية والمكورات العنقودية الذهبية ذات الاحتمالية المرضية المعزولة من الحليب الخام للابل والجاموس بالطرق الاعتيادية والجزيئية == Identification And Molecular Characterization of Potential Pathogenic Staphylococcus Aureus And Escherchia Coli Isolated From She - Camel And Buffalo Raw Milk

Author name: اسراء تلفان عناد
Supervisor name: باسل عبد الزهرة عباس | بسام ياسين خضير
General topic: Veterinary Medicine
Degree: Master
Language: English
University location: Basrah
First pages:
Abstract: تم جمع 430 عينة ,215 عينة من حليب الجمال الخام جمعت من مناطق مختلفه من محافظة البصرة ومحافظات بالمنطقة الجنوبية للعراق و215عينة من حليب الجاموس الخام جمعت من مناطق مختلفة من محافظة البصرة خلال فترة ثمانية اشهر(اب 2012 - اذار 2013) حيث تم جمع الحليب يدويا | During a period of eight months (August 2012 to March 2013), a total of 430 samples were collected from 215 camel milk samples, 215 buffalo milk samples from different regions in Basrah city and other cites in southern area in Iraq. All specimens were screened for the presence of E. coli and S.aureus by cultured on MAC, EMB and MS agar media.A total of 51 (11.8 %) of suspected E. coli isolates were obtained; 23 (10.6%) from camel milk samples and 28 (13.0%) from buffalo milk samples. The study showed higher occurrence of E. coli in buffalo milk samples than camel milk samples. All suspected E. coli isolates were tested with Api 20 and 35(68.6%) were confirmed as E. coli. Staphylococcus aureus were also isolated as suspected staph, A total of 39 (9.06) were obtained; 17 (7.9) from camel milk samples and 22 (10.2) from buffalo milk samples. The study showed higher occurrence of S.aureus in buffalo milk samples followed by camel milk samples. After test the suspected staph with api test appear 27 (69.2) were confirmed as S.aureus.All the isolates of E.coli and S. aureus were tested to at least 12 antibiotics to which they were subjected. All the isolated bacteria (100%) of E. coli isolates were susceptible to gentamycin,imipenem and amikacin but resistant (100%) to amoxicillin, trimethoprim, azithromycin ,amoxicillin/clavulanic acid, cephalothin, cefixime and cefoxitin. Some of them (50%) were resistance to nalidixic acid. Three isolates 75% were resistance to tetracycline whereas 75% were susceptible to ciprofloxacin, and nitrofurantoin. Intermediate susceptibility to ciprofloxacin ,nalidaxic acid , nitrofurantoin and tetracyclin was observed among 25% of the E. coli isolates whereas 25% showed resistance to nalidixic acid. Therefore, all these isolates were considered to be multidrug resistant.E. coli were further examined by PCR technique using two pairs of primers to amplify both itsf and pai genes. The results revealed that all (85.7%) of the isolates were positive for itsf gene, 57.1% of isolates yielded amplification products with Pai - PCR gene. Staph aureus examined to presence of mecA gene by technique using pairs of primers and it was found that 51.8% of strain contain that gene. Clustalw multiple sequence alignment of icd gene of E.coli and arcC gene of S.aureus isolates and three previously reported E.coli and S.aureus was done.

دراسة مقارنة لخمائر المبيضات المعزولة من الابقار والنساء مع التحري الجزيئي لبعض جينات الضراوة == Comparative Study Of Candida Species Isolated From Cows And Women With Molecular Detection Of Some Virulence Genes

Author name: رغد شريف ابراهيم
Supervisor name: باسل عبد الزهرة عباس
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

توصيف جراثيم السالمونيلا المعزولة من الدجاج اللاحم باستخدام التحليل البكتريولوجي, المصلي, سلسلة البلمرة المتعدده وقياس الحساسية الدوائية == Characterization of Salmonella spp. isolated from broiler chickens by using bacteriological, serotyping, PCR and antimicrobial susceptibility analysis

Author name: نائل مهدي عوفي
Supervisor name: محمد حسن خضر | علي عبود العيداني
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

التحري عن فايروس جدري الاغنام باستخدام تفاعلات سلسلة البلمرة وفحص المقياس المناعي المرتبط بالانزيم غير المباشر == Detection of sheeppoxvirus by PCR and indirect ELISA

Author name: مسلم ظاهر موسى
Supervisor name: فوزية علي عبد الله
General topic: Veterinary Medicine
Specific topic: Microbiology - Viruses
Degree: Master
Language: English
University location: Basrah
First pages:

الجوانب البكتريولوجية الجزيئية والفعالية الذيفانية لمنحنيات الصائم المعزولة من الابقار والاغنام المجزورة في البصرة == Bacteriological, Molecular and toxogenic activity Aspects of Campylobacter jejuni isolated from slaughtered cattle and sheep at Basrah city

Author name: لمياء يوسف راضي
Supervisor name: علي عبود عيسى | محمد حسن خضر
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

الكشف عن جينات rfbO157 وflicH7 في بكتيريا الاشريشيا القولونية المعزولة من البشر والاغنام في محافظة البصرة == Detection of rfbO157 and fliCH7 genes in Escherichia coli isolated from Human and Sheep in Basrah Province

Author name: فاروق لطيف جاسم الحسناوي
Supervisor name: عواطف حميد عيسى | محمد حسن خضر
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

الكشف الجزيئي لجينات البيتالاكتاميز (tem and shv) في جرثومة الاشرشيا القولونية (O157 : H7)المعزولة من الانسان والحيوان == Molecular Detection of ? - lactamase Genes(tem and shv) in Escherichia coli O157 : H7 Isolated from Human and Animals.

Author name: خلود عبد الرزاق خليل
Supervisor name: باسل عبد الزهرة عباس | بسام ياسين خضير
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

الكشف عن جينات السموم المعوية ( (see - sea في جرثومة المكورات العنقودية الذهبية المعزولة من الحليب الخام بوساطة تقنية تفاعل البلمرة المتعدد ودراسة امراضيتها == Detection of enterotoxin genes (sea - see) of Staphylococcus aureus isolated from raw milk by multiplex PCR and study of their pathogenicity

Author name: حسن اكريم ادبيس
Supervisor name: باسل عبد الزهرة عباس | محمد حسن خضر
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

الكشف الجزيئي للجينات المشفرة للذيفانات المنتجة من بكتريا Bacillus cereus المعزولة من الحليب ومشتقاته في محافظة البصرة == Molecular Detection of Genes Encoding to Toxins Produced by Bacillus cereus Isolated from Milk and Its Products in Basrah Province

Author name: بان محمد صالح سـعيد
Supervisor name: باسل عبد الزهرة عباس | محمد حسن خضر
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

عزل بعض الفطريات من تربة مناطق هور الحمار والكشف عن انتاج مضاد حيوي من عزلة الفطر Penicillium Aethiopicum Frisvad == Isolation of Some Fungi from Hammar Marsh Areas Soil and Production Antibiotic from Detection of Penicillium aethiopicum Frisva

Author name: ضرغام علي حسن
Supervisor name: علي اسماعيل عبيد السنافي | فوزية علي عبد الله
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة بايولوجية وجزيئية لطفيلي الجيارديا كمنشا مشترك في الانسان والحيوان في شمال البصرة == Biological And Molecular Study Of Giardia Parasite As A Zoonotic Potential In Human And Animal At Northern Basrah

Author name: رشا خليل عبد الجليل السعد
Supervisor name: غازي يعقوب عزال الامارة
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Basrah
First pages:

عزل وتشخيص جرثومة Salmonella Typhimurium والكشف عن الجين المشفرلتخليق النوع الهدبي الاول باستعمال تقنية تفاعل سلسلة البوليمريز == Isolation and Identification of Salmonella typhimurium and detection of gene encoded Type - 1 - fimbriae by using Polymerase Chain Reaction

Author name: حيدر علي محمد الكرعاوي
Supervisor name: عدنان موسى الروضان | عدنان حمد الحمداني
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة تشخيصية ونسيجية لديدان الرئة والاكياس المائية في اغنام العواسي

Author name: حنان عبد الزهرة صلبوخ المنصوري
Supervisor name: منى محمد جوري
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

مستارجات قشرة القطط والكلاب كمسببات للنوع الاول من فرط الحساسية في مرضى الربو والحساسية == Cats And Dogs Dander Allergens Causing Type I Hypersensitivity In Asthmatic And Other Allergic Patients

Author name: عبير ليلي محمد
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Basrah
First pages:

تطبيق طريقة التحول الوراثي في نقل صفة انتاج مضاد حيوي من بكتريا Streptomyces sp الى Escherichia coli pBR322 == Application Of Transformation Method In Transfer Of Antibiotic Production Property From Streptomyces Sp. To Escherichia Coli pBR322

Author name: مرتقب يونس عبيد الحجاج
General topic: Veterinary Medicine
Degree: Master
Language: English
University location: Basrah
First pages:

دور مستارجات حليب المعز والجاموس كمسبب للنوع الاول من فرط الحساسية وعلاقتهما التصالبيه مع مستارجات حليب الابقار == The Role Of Goat’s And Buffalo’s Milk Allergens As Causative Agents Of Type I Hypersensitivity And Their Cross - Reactivity With Cow’s Milk Allergens

Author name: رنا عدنان فائز
General topic: Veterinary Medicine
Specific topic: Microbiology - Immunology
Degree: Master
Language: English
University location: Basrah
First pages:

عزل وتشخيص جرثومة Burkholderia pseudomallei من حليب الابقار والمعز والبيئة المحيطة بها في محافظة البصرة == Isolation and Identification of Burkholderia pseudomallei from Cow and Goat’s Milk and Their Surrounding Environment in Basrah Province

Author name: هناء خليل ابراهيم
Supervisor name: عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages:

Extraction And Purification Of Salmonella Spp. Enterotoxin Isolated From Bovine In Basrah Province

Author name: مؤيد حنون صيهود
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Basrah
First pages:

عزل وتشخيص بعض جراثيم الفلورا الطبيعية من كرش وامعاء بعض المجترات والكشف عن الفعالية الضد ميكروبية لجرثومة الايشيريكيا القولونية == Isolation and Identification of Some Normal Flora From The Rumen and Intestine of Some Ruminants and Detection of The Antimicrobial Activity of Escherichia coli

Author name: عبد العزيز صالح الشمري
Supervisor name: داوود سلمان علي | عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages: