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التحري عن داء المقوسات في النساء والنعاج بواسطة طرق مصلية مختلفة في محافظة ميسان - العراق == An Investigation of Toxoplasmosis In Women And Ewes By Different Serological Assays In Maysan Governorate, Iraq
Author name:
مرتضى شهاب جبار
Supervisor name:
منى محمد جوري
General topic:
Veterinary Medicine
Specific topic:
Microbiology - Parasites
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Microbiology And Veterinary Parasites
Language:
English
University location:
Basrah
First pages:
21T502 - p.pdf
Abstract:
تم جمع 291 عينة من الدم الوريدي من النساء في سبعة مناطق مختلفة في محافظة ميسان للفترة من تشرين الثاني 2012 وحتى اذار 2013 للكشف عن داء المقوسات باستخدام ثلاثة اختبارات هي اختبار التلازن الحبيبي وانزيم الادمصاص المناعي واختبار المنفايدس ثلاثة مجاميع من الن | In this study, venous blood sample were collected from 291 women in seven different regions of Maysan province during the period from October 2012 to March 2013 for detecting the toxoplasmosis by using three tests (Latex agglutination (LAT), enzyme linked immunosorbent (ELISA - IgG) and minividas. Three groups of women ( pregnant ,non - pregnant and aborted ) and 283 ewes which represent 18 herds from nine regions of Maysan province were selected for the study in order to diagnose the toxoplasmosis by using ELISA and LAT tests. The results showed that the ratio of Toxoplasma seropositivity in aborted women was significantly the highest (69.23%) followed by pregnant group (60.16%) by using LAT. Moreover, the ratio of seropositivity was highest in aborted women (47.43%) followed by pregnant (36.58%) and non - pregnant (26.66%) groups, respectively by using ELISA test. With Minividas assay, the results showed the highest seropositivity percentage in aborted women (26.92%) followed by the pregnant (17.88%) and non - pregnant (11.11%) groups, respectively. According to regions of the study, there was higher seropositivity against T. gondii in women in Al - Musharah subdistrict by LAT (68.09%) and ELISA (44.68%) test, respectively. However, the seropositivity by using minividas assay in three regions in AL - Majar district, Al - Musharah subdistrict and AL - Maymona district were 23.52% , 23.40% , 23.52% , respectively in comparison with other regions in Maysan governorate. The significantly high (P? 0.05) seropositivity detected by LAT (72.63%), ELISA (50.52%) and minividas (28.42%) were in women of age group > 30 years. The results showed that the ratios of Toxoplasma seropositivity in aborted ewes group was the highest (73.33%) followed by pregnant group (65.38%) after using the LAT, while the seropositivity in aborted ewes detected by the ELISA test was 40%. The results from Al - Musharah subdistrict that showed highest seropositivity against Toxoplasma by using LAT and ELISA tests were 78.26% and 39.13%, respectively. The highest Toxoplasma seropositivity in the two - years age - group ewes was 65.57% and one - year age - group was 65.30% after using LAT test, while was 32.65% in the age - group more than a year by ELISA. The statistical analysis showed significant difference (p ? 0.05) between the LAT and ELFA tests conducted for women samples, but there were no significant differences (p? 0.05) between LAT and ELISA carried out for women and ewes samples with regarded to Toxoplasma seropositivity.
