البحث — المستودع الرقمي العراقي

نتائج البحث: 18 من أصل 18

التقييم المناعي للقاحات المحضرة من العزلة المحلية للمكورات العنقودية المقاومة للميثيسيلين Immunological Evaluation of Vaccines Prepared from Local Isolate Methicillin Resistant Staphylococcus aureus

اسم المؤلف: عبد الله عبد العزيز خليل شيحان

اسم المشرف: محمد حسن خضر فنار ابلحد اسحق

الموضوع العام: الطب البيطري

السنة: 2022

الموضوع الدقيق: الاحياء المجهرية

الدرجة: دكتوراه

الجامعة: جامعة البصرة - كلية الطب البيطري - قسم الاحياء المجهرية والطفيليات البيطرية

اللغة: الانكليزية

مكان الجامعة: البصرة

الصفحات الاولى:

الكشف عن انواع الايميريا في الاغنام والماعز في بعض مناطق محافظة البصرة جنوب العراق Detection of Eimeria spp. in Sheep and Goats in some areas of Basrah Province, Southern Iraq

اسم المؤلف: هناء عبد الحسين شهيد

اسم المشرف: سوزان عبد الجبار عبد العزيز

الموضوع العام: الطب البيطري

السنة: 2021

الموضوع الدقيق: الطفيليات

الدرجة: ماجستير

الجامعة: جامعة البصرة - كلية الطب البيطري - قسم الاحياء المجهرية والطفيليات البيطرية

اللغة: الانكليزية

مكان الجامعة: البصرة

الصفحات الاولى:

الكشف السيرولوجي والجزيئي لفايروس مرض الحمى القلاعية في الابقار في محافظة البصرة Serological And Molecular Detection of Fmdv In Cow of Basrah Province

اسم المؤلف: زينب مجيد سالم الكلي

اسم المشرف: عدنان موسى الروضان

الموضوع العام: الطب البيطري

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - التقنية الحيوية

الدرجة: ماجستير

الجامعة: جامعة البصرة - كلية الطب البيطري - قسم الاحياء المجهرية والطفيليات البيطرية

اللغة: الانكليزية

مكان الجامعة: البصرة

الصفحات الاولى:

المستخلص: اجريت هذه الدراسة في6 مناطق مختلفة ( شط العرب والزبير والقرنه وابي الخصيب ومركز البصره والمدينه) ممثله لجميع اجزاء محافظة البصره خلال الفتره من شهر تشرين الاول من العام 2012 الى شهر تموز من عام 2013 ولتحديد نسبة انتشار مرض الحمى القلاعيه في الابقار استخد | This study was conducted on local cattle from 6 different regions (Shutt - Alarab,Al - Zubair, Al - Qurna, Abi - Elkhasib, Basrah center and Almdaina) covering all parts of the Basrah province during the period from October 2012 to July 2013. In order to determine the prevalence of foot and mouth disease in bovine species Enzyme Linked Immune Sorbent Assay ( ELISA) and reverse transcriptase polymerase chain reaction techniques( RT - PCR) were used in the testing of serum ,saliva and vesicular fluid samples collected from clinically diseased cattle, moreover this study was also mentioned some clinical aspects of the disease.Diseased animals showed the following clinical signs : depression(92.1%),sever salivation(90.9%) ,vesicles in mouth(90.3%), fever(89.1%) , conjunctivitis(87.9%) and smacking of lips in higher rates( 80.6%).While the vesicles on teat(12.7%), lameness(36.9%)and vesicles on feet (45.5%)appeared in lower rates.The overall prevalence of this disease was(72.7%.).Of 165 serum samples subjected to nonstructural protein (NSP) ELISA screening test (ELISA CHEKIT FMD - 3ABC Bo - Ov ELISA Test ) , 120 were interpreted as positive representing (72.7%.) while the other 45samples were negative representing 27.3%.The seropositivity significantly associated with age groups (p<0.01) and sex (P < 0.01).Depending on sex of these animals , the higher seropositivity rate was observed in females (78.6%).Concerning the age of tested cattle the animals of 2nd age group (>4 - 8 years) appeared in higher rate (84.8 %) of seropositivity followed by the rate (51.7% ) of 1st age group animals(<1 - 4 years).The sero - positivity against FMDV was non significantly different(>P 0.05)among the cattle in Basrah districts and highest sero - prevalence rate was observed in cattle of Basrah center (100%), Al - Mdaina (77.8%), Shut - Alarab(76.3%) and Abi - Elkhasib (70%).The lower sero - positivity rate was observed in Qurna(58.8%)and Zubair (50%) cattle.RT - PCR detection of FMDV for primary and serotype specific diagnosis was used. of eighty three clinically positive samples including, mouth vesicles, saliva and serum samples tested by RT - PCR, only 68(81.9%) were successfully amplified, their identification was done with universal primer sets (1F / 1R, )with expected band of( 328 bp).Of sixty eight universal primer based RT - PCR positive samples tested for serotype A - 1C562(865bp), O - 1C272( 635bp ) and Asia1 - 1C505(911bp) serotypes primers, 19(27.9%) samples were found to be serotype A - 1C562 positive and 26(38.2%) were serotype O - 1C272 positive and nil for Asia1 - 1C505 serotype. The frequency of foot - and - mouth disease viral genome presence in cattle had significant differences concerning the sex (P<0.05) while the difference among age groups was highly significant (P < 0.01) in case of universal gene and not significant(P>0.05) in case of all serotypes genes. Depending on the sex effect, the rate of RT - PCR positive results of universal gene and O - 1C272 serotype gene were higher in females (87.5 and38.8% respectively ),while A - 1C562 serotype RT - PCR positivity rate was higher in males (31.6% ).Different rates of RT - PCR positivity was observed in the two age groups of cattle and the second age group(>4 - 8 year) showed higher rate of positivity as a follow : (universal primer (90.7%),O - 1C272 serotype primer( 40.8%) and A - 1C562 serotype primer(34.7% ).In the studied regions of Basrah province , the FMDV genes were significantly(P<0.05)distributed. The rates of universal gene appearance were100, 93.3 and 83.3%of cattle in Zubair ,Qurna and Shutt - Alarab respectively. The serotype A - 1C562 was distributed in higher rate ( 100 and 40%) of Zubair and Al - Mdaina cattle respectively , while the serotype O - 1C272 was observed in higher distribution rate in cattle of Abi - Elkhasib (44.4%) and Qurna(42.9%), so there is significant differences ((P<0.05) according to distribution of serotypes of FMDV of regions of Basrah province.

التحري عن داء المقوسات في النساء والنعاج بواسطة طرق مصلية مختلفة في محافظة ميسان - العراق An Investigation of Toxoplasmosis In Women And Ewes By Different Serological Assays In Maysan Governorate, Iraq

اسم المؤلف: مرتضى شهاب جبار

اسم المشرف: منى محمد جوري

الموضوع العام: الطب البيطري

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

الدرجة: ماجستير

الجامعة: جامعة البصرة - كلية الطب البيطري - قسم الاحياء المجهرية والطفيليات البيطرية

اللغة: الانكليزية

مكان الجامعة: البصرة

الصفحات الاولى:

المستخلص: تم جمع 291 عينة من الدم الوريدي من النساء في سبعة مناطق مختلفة في محافظة ميسان للفترة من تشرين الثاني 2012 وحتى اذار 2013 للكشف عن داء المقوسات باستخدام ثلاثة اختبارات هي اختبار التلازن الحبيبي وانزيم الادمصاص المناعي واختبار المنفايدس ثلاثة مجاميع من الن | In this study, venous blood sample were collected from 291 women in seven different regions of Maysan province during the period from October 2012 to March 2013 for detecting the toxoplasmosis by using three tests (Latex agglutination (LAT), enzyme linked immunosorbent (ELISA - IgG) and minividas. Three groups of women ( pregnant ,non - pregnant and aborted ) and 283 ewes which represent 18 herds from nine regions of Maysan province were selected for the study in order to diagnose the toxoplasmosis by using ELISA and LAT tests. The results showed that the ratio of Toxoplasma seropositivity in aborted women was significantly the highest (69.23%) followed by pregnant group (60.16%) by using LAT. Moreover, the ratio of seropositivity was highest in aborted women (47.43%) followed by pregnant (36.58%) and non - pregnant (26.66%) groups, respectively by using ELISA test. With Minividas assay, the results showed the highest seropositivity percentage in aborted women (26.92%) followed by the pregnant (17.88%) and non - pregnant (11.11%) groups, respectively. According to regions of the study, there was higher seropositivity against T. gondii in women in Al - Musharah subdistrict by LAT (68.09%) and ELISA (44.68%) test, respectively. However, the seropositivity by using minividas assay in three regions in AL - Majar district, Al - Musharah subdistrict and AL - Maymona district were 23.52% , 23.40% , 23.52% , respectively in comparison with other regions in Maysan governorate. The significantly high (P? 0.05) seropositivity detected by LAT (72.63%), ELISA (50.52%) and minividas (28.42%) were in women of age group > 30 years. The results showed that the ratios of Toxoplasma seropositivity in aborted ewes group was the highest (73.33%) followed by pregnant group (65.38%) after using the LAT, while the seropositivity in aborted ewes detected by the ELISA test was 40%. The results from Al - Musharah subdistrict that showed highest seropositivity against Toxoplasma by using LAT and ELISA tests were 78.26% and 39.13%, respectively. The highest Toxoplasma seropositivity in the two - years age - group ewes was 65.57% and one - year age - group was 65.30% after using LAT test, while was 32.65% in the age - group more than a year by ELISA. The statistical analysis showed significant difference (p ? 0.05) between the LAT and ELFA tests conducted for women samples, but there were no significant differences (p? 0.05) between LAT and ELISA carried out for women and ewes samples with regarded to Toxoplasma seropositivity.

تشخيص وتوصيف جراثيم الاشريشيا القولونية والمكورات العنقودية الذهبية ذات الاحتمالية المرضية المعزولة من الحليب الخام للابل والجاموس بالطرق الاعتيادية والجزيئية Identification And Molecular Characterization of Potential Pathogenic Staphylococcus Aureus And Escherchia Coli Isolated From She - Camel And Buffalo Raw Milk

اسم المؤلف: اسراء تلفان عناد

اسم المشرف: باسل عبد الزهرة عباس بسام ياسين خضير

الموضوع العام: الطب البيطري

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - التقنية الحيوية

الدرجة: ماجستير

الجامعة: جامعة البصرة - كلية الطب البيطري - قسم الاحياء المجهرية والطفيليات البيطرية

اللغة: الانكليزية

مكان الجامعة: البصرة

الصفحات الاولى:

المستخلص: تم جمع 430 عينة ,215 عينة من حليب الجمال الخام جمعت من مناطق مختلفه من محافظة البصرة ومحافظات بالمنطقة الجنوبية للعراق و215عينة من حليب الجاموس الخام جمعت من مناطق مختلفة من محافظة البصرة خلال فترة ثمانية اشهر(اب 2012 - اذار 2013) حيث تم جمع الحليب يدويا | During a period of eight months (August 2012 to March 2013), a total of 430 samples were collected from 215 camel milk samples, 215 buffalo milk samples from different regions in Basrah city and other cites in southern area in Iraq. All specimens were screened for the presence of E. coli and S.aureus by cultured on MAC, EMB and MS agar media.A total of 51 (11.8 %) of suspected E. coli isolates were obtained; 23 (10.6%) from camel milk samples and 28 (13.0%) from buffalo milk samples. The study showed higher occurrence of E. coli in buffalo milk samples than camel milk samples. All suspected E. coli isolates were tested with Api 20 and 35(68.6%) were confirmed as E. coli. Staphylococcus aureus were also isolated as suspected staph, A total of 39 (9.06) were obtained; 17 (7.9) from camel milk samples and 22 (10.2) from buffalo milk samples. The study showed higher occurrence of S.aureus in buffalo milk samples followed by camel milk samples. After test the suspected staph with api test appear 27 (69.2) were confirmed as S.aureus.All the isolates of E.coli and S. aureus were tested to at least 12 antibiotics to which they were subjected. All the isolated bacteria (100%) of E. coli isolates were susceptible to gentamycin,imipenem and amikacin but resistant (100%) to amoxicillin, trimethoprim, azithromycin ,amoxicillin/clavulanic acid, cephalothin, cefixime and cefoxitin. Some of them (50%) were resistance to nalidixic acid. Three isolates 75% were resistance to tetracycline whereas 75% were susceptible to ciprofloxacin, and nitrofurantoin. Intermediate susceptibility to ciprofloxacin ,nalidaxic acid , nitrofurantoin and tetracyclin was observed among 25% of the E. coli isolates whereas 25% showed resistance to nalidixic acid. Therefore, all these isolates were considered to be multidrug resistant.E. coli were further examined by PCR technique using two pairs of primers to amplify both itsf and pai genes. The results revealed that all (85.7%) of the isolates were positive for itsf gene, 57.1% of isolates yielded amplification products with Pai - PCR gene. Staph aureus examined to presence of mecA gene by technique using pairs of primers and it was found that 51.8% of strain contain that gene. Clustalw multiple sequence alignment of icd gene of E.coli and arcC gene of S.aureus isolates and three previously reported E.coli and S.aureus was done.