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اليات استحثاث المقاومة في نبات الخيار بواسطة Trichoderma harzianum وBacillus subtilis وGlomus mosseae اتجاه الفطر Rhizoctonia solani المسبب لمرض سقوط البادرات == Resistance Mechanisms In Cucumber Plant By Trichoderma Harzianum, Bacillus Subtilis And Glomus Mosseae Against Rhizoctonia Solani Causing Seedling Disease

اسم المؤلف: رباب مجيد عبد الذهيبي
اسم المشرف: علي هاشم الموسوي | هادي مهدي عبود
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نفذت هذه الدراسة للتحري عن اليات استحثاث المقاومة في نبات الخيار ضد الفطر الممرض Rhizoctonia solani بوساطة المعاملة بالعوامل الاحيائية وهي harzianum Trichodermaو Bacillus subtilis وGlomus mosseae وتاثير ذلك في نمو نبات الخيار وحاصله النامي تحت ظروف البيت | This study was carried out to investigate the mechanisms of induced resistance in cucumber plant against pathogenic fungus Rhizoctonia solani by bioeagent factors Tricoderma harzianum fungus, Basillus sibtilis bacteria and mycorrhizal fungus Glomus mosseae. The effect in parameters growth and yield of cucumber plant which growing under greenhouse and field conditions. The pathogenic fungus showed high Pathogenesis on cucumber plant. Three isolated of B. subtilis and T. harzianum showed high antagonism ability against R. solani under laboratory condition. For mycorrhizal fungus G. mosseae isolated was tested to study their ability in cucumber growth for chosen the best isolated which can used in anther experiment which results were as follows : - A - Pots experiment 1 - The bioeagent factors was showed their ability to induced several mechanisms of resistance in cucumber plant against pathogenic fungus R. solani, Which included increasing the activity of defense enzymes in existed of pathogenic fungus R. solani, The treatment (T. harzianum + G. mosseae) was recorded highest level in enzyme activity of Phenylalanine ammonia layase enzyme which was (25.3541) unit/gm fresh weight, While T. harzianum treatment showed significantly increased the enzyme activity of Polyphenol oxidase and Peroxidase which was (10.6707, 2.6137) unit/gm fresh weight respectively. This study showed no enzyme activity for chitinase enzyme excepted in some treatment and some periods.This study recorded increased in plant content of total phenol, lignin, salicylic acid hormone, total protein, The (G. mosseae + B. subtilis) treatment overtaken on other treatments in increased plant content of total phenol which was (1.989) mg/gm fresh weight, B. subtilis treatment was significantly increased plant content of lignin which was (18.318) mg/gm fresh weight, The treatment (T.harzianum+ B. subtilis) showed significantly increased plant content of salicylic acid hormone which was (1.8658) mg/gm fresh weight. This study recorded no Jasmonic acid hormone activity in treated plant except in some treatments but with low level, The treatment (G. mosseae + T.harzianum + B. subtilis) was recorded highest content in total protein which was (239.3) ?g/mg. 2 - The rate of seedling death and disease index was significantly reduced in bioeagent treatments, The lowest rate for seedling death in the treatment of (G. mosseae + B. subtilis), (G. mosseae + T.harzianum) and (G. mosseae + T.harzianum + B. subtilis) which were 11 % each for them comparison with control treatmentwith pathogen which was 100%.3 - The rate number of spores and percentage of mycorrhiza infection was significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. The treatment of mycorrhiza infection with pathogen recorded highest number of mycorrhizal spores which was (1410) spore. The mycorrhiza infection reach to 100% in (G. mosseae + T.harzianum) and(G. mosseae + T.harzianum + B. subtilis) treatment with pathogen fungus. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest index of mycorrhiza infection which reach to (80)%, But the index of mycorrhiza infection and mycorrhizal root weight were decreased in the existed of pathogenic fungus, Mycorrhizal dependency it was increased in all treatments with pathogenic fungus and the treatment of (G. mosseae + B. subtilis) with pathogen recorded highest percentage in Mycorrhizal dependency which was (58.873)%. 4 - The plant content of chlorophyll (a, b, total) was significantly increased, The tratment of (G. mosseae + B. subtilis) with pathogen recorded highest level in chlorophyll a it was (5.30)mg/gm, the highest level in chlorophyll b found in(G. mosseae + T.harzianum + B. subtilis) without pathogen it was (6.07)mg/gm, (G. mosseae + T.harzianum + B. subtilis) treatment without pathogen recorded highest level in total chlorophyll it was (10.79)mg/gm. The concentration of phosphor concentration increased in shoot part of (G. mosseae + T.harzianum) without pathogen which was (0.538)%, potassium concentration were decreased in all treatments with pathogen excepted (G. mosseae + T.harzianum) treatment, The highest level of potassium in treatment of (B. subtilis) without pathogen which recorded (4.65)%, For nitrogen concentration was increased in all treatments with pathogen and the highest level in (T.harzianum) which was (5.114)%. Carbon concentration significantly decreased in all treatments with pathogen and (G. mosseae + B. subtilis) without pathogen recorded highest concentration in carbon which was (46.203)%. 5 - The result showed that all growth parameters either in the absence or presence of pathogenic fungus was significantly increased in all bioagent treatments.The percentage of germination reach to (100)% in (B. subtilis) with or without pathogen , the treatment (G. mosseae + B. subtilis) with pathogen recorded highest level in shoot length it was (92.40)cm, And the same treatment recorded highest rate in fresh and dry weight for shoot part which was (7.412, 0.990) gm respectively, (T.harzianum) without pathogen recorded highest rate in leaf area it was (453.083)cm2, The highest rate of flower number was in (T.harzianum) with pathogen which was (15.66)flower/plant. The fresh and dry weight for root, root length, root surface area and the rate of root diameter were significantly increased in comparison with control treatment without pathogen. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest level in fresh and dry weight for root which were (0.359, 0.094)gm respectively. Highest root length in (G. mosseae + B. subtilis) without pathogen it was (48.14)cm, root surface area decreased in the treatments with pathogen and the treatment (G. mosseae + T.harzianum + B. subtilis) without pathogen recorded highest root surface area it was (19.16)cm 2, The root diameter was increased in(G. mosseae) with pathogen was reach to (0.363)mm.B - Field experiment 1 - Under field condition the percentage of germination increased in the treatment without pathogen which were (94.44)% in treatments (G. mosseae) , (B. subtilis) and (G. mosseae + T.harzianum) respectively, In the treatments with pathogen the percentage of germination reduced and The bioeagent factors showed no reduction in percentage of disease infection or disease index with pathogenic fungi R. solani under field condition which reach to (100)%. 2 - The rate number of spores, percentage of mycorrhiza infection, index of mycorrhiza infection, mycorrhizal root weight and Mycorrhizal dependency on cucumber plant significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. (G. mosseae + T.harzianum + B. subtilis) significantly overtaken on other treatments in increased rate of mycorrhizal spors it was (3950)spore, The mycorrhiza infection reach to (100)% in all treatments, For index of mycorrhiza infection the treatments of (G. mosseae + B. subtilis) and (G. mosseae + T.harzianum) recorded highest rate which reach to (100)%, (G. mosseae + T.harzianum + B. subtilis) treatment recorded high increased in mycorrhizal root weight and Mycorrhizal dependency which were (545.67 gm, 67.78%) respectively. 3 - The plant content of total chlorophyll was significantly increased, The highest level in(G. mosseae + T.harzianum + B. subtilis) treatment which was 5.19 mg/gm. There was significantly increased in the concentration of nutrition elements P, K, N, C in cucumber plant. The treatment (T.harzianum+ B. subtilis) recorded highest concentration in phosphor and nitrogen which were (489.4, 3.62) % respectively, The treatment of (G. mosseae + T.harzianum + B. subtilis) recorded highest concentration of potassium which was (2.975)%. The highest concentration of carbon recorded in B. subtilis treatment which was (34.823) %. 4 - All shoot, root and yield parameters of cucumber were significantly increased under field conditions. The treatment (T.harzianum+ B. subtilis) recorded significantly increased in plant height, dry shoot weight, leaf area rate number of flowers, rate number of fruit and fruit weight which were (42.87 cm, 10.85 gm, 631.06 cm2, 19, 17, 1275 gm) respectively. The treatment G. mosseae recorded significantly increased in fresh shoot weight which was (32.76) gm. The treatment with (T.harzianum+ B. subtilis) recorded significantly increased in fresh and dry weight of root, root length, root surface area, rate of root diameter, rate number of fruit and fruit weight which were (11.4 gm, 5.5 gm, 151.71 cm, 44.05 cm2, 0.763 mm, 17, 1275 gm) respectively,

دراسة مقارنة لتاثير بعض المستخلصات النباتية وبعض الاحماض الكاربوكسيلية في البكتريا المسببة لالتهابات الحروق == Comparative Study of The Effect of Some Plants Extract And Carboxylic Acids On Contaminating Bacteria In Burns Infection

اسم المؤلف: سـعاد خليـل ابراهيـم
اسم المشرف: مهدي ضمد القيسي | جنان مجيد العقيدي
الموضوع العام: علوم الحياة
السنة: 2009
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت (500) عينة من المرضى المصابين بالحروق من ثلاث مستشفيات في مدينة بغداد للمدة من 1/6/2007 ولغاية 1/11/2007. زرعت هذه المسحات على الاوساط الزرعية للعزل الاولى وتم تشخيصها بالفحوصات المظهرية والبايوكيميائية. اظهرت (489) عينة ملوثة بالبكتريا وبنسبة | • Five hundreds swabs were collected from patients suffered from burns in three hospitals of Baghdad city, for the period from 1st June 2006 to 1st November 2007. The swabs were implanted in order to be isolated and diagnosed by morphological and biochemical tests. • The results showed that 489 swabs were contaminated with bacteria (97.8%), while 11 swabs were not (2.2%). The identification and prevalence of the bacteria was as follow Pseudomonas aeruginosa was found in 148 swabs (30.27%), Klebsiella pneumoniae in 102 (20.26%), Staphylococcus aureus in 79 (16.16%), Escherichia coli in 70 (14.31%), Proteus mirabilis in 52 (10.63%) and Enterobacter cloacae in 38 (7.77%). • Information card for each patient was assigned included age, sex, time of suffering from burns, social status and address.• The degrees of suffering were as follow : - First degree of burns, 201 cases (40.2%). - Second degree, 187 cases (37.4%). - Third degree, 112 cases (22.4). Sensivity test to antibiotic and was done for all the isolated bacteria, and it was found, that, they were sensitive to Vancomycin, Ciprofloxacin, Amikacin, Tetracyclin and Fusidic acid within the percentage ratio of 0.0, 59.1, 3.7, 58.5 and 11.6%, respectively. Also it was found that the investigated bacteria showed high Vesistance to Penicillin G (87.9%), Erythromycin (93.9%), Trimethoprim (100%), Carbencillin (83.9), Cefotaxime (63.4%), Ampicillin (75.9%), Gentamycin (67.9%), Streptomycin (83.6%), Nalidix acid (89.4%) and Linomycin (83.2%). o Searching for the active compounds in the extracts of investigated plants (Linum usitatissimum, Nigella sativa and Eruca sativa) was conducted. It was found, that, the seeds of the three plants contained Glycosides, Alkalis, Flavonoids, Soapiness and Resins, which are not water soluble, but could be detected by the alcoholic extraction. Cumarin was noticed in the seeds of N. sativa and E. sativa only. o Different concentrations of water and alcohol extracts were prepared in addition to the isolation of oils, soapiness and Flavonids. The effectiveness of the extracts was tested at the concentration of 16, 32, 64, 128, 256 mg/ml for all the plants, against the bacteria.o The Phenol, water and alcohol extracts of L. usitatissimum and E. sativa showed high effect against the bacterial species, while that of N. sativa was less affective.o The Minimum inhibition concentration (MIC), was found to be different according to the type of extract and the species of bacteria.o The effects of the organic acids (Acetic, Citric, and Lactic) were tested at concentrations of 0.5, 1, 1.5, 2, 3 and 4% of each acid. An increase in the diameter of inhibition area, related with the increase in the acid concentrations, was detected. It was found that the acetic acid was more affective against the microorganisms, followed by the Citric acid and then the Lactic acid.o on the base of ratios of inhibition area diameters (in vitro), the results illustrated that E. coli was more affected by Acetic acid when the diameters reached 18.75, 21.75, 30.75, 38.25, 40.00 and 44.75 mm, respectively. The concentration of 1.5% was the effective enough against E. coli as diameters rates for P. mirabilis were 19.25, 18.25, 18.0, 22.56, 27.75 and 31.0 mm, respectively, followed by Ps. aeruginosa, S. aurues and En. cloacae when exposed to the optimum concentrations of Acetic, Citric, Lactic acids. The diameters at concentration of 4% were 23.50 and 23.66 mm. o The water extracts from all the involved plants, in addition to the organic acids showed good levels of effectiveness against the different studied bacterial species, when suffered by burns rats were exposed to them. A clear decrease in bacteria numbers in these rats in comparison with non - treated animals, in the swabs removed from the treated and non - treated rats, in addition to the absence of any histophathalogical changes in livers and skins of the treated animals. o Biochemical tests of serums from animals treated with the water extracts and organic acids at concentration of 16 mg/Kg and 18 mg/Kg for seven days and the microscope investigation, proved that these compounds were not toxic.o The effects of the water extracts and the organic acids were studied too from the point of the immunity point of view. An increase in the Levels of IgG and IgA of the supplementary systems C3 and C4 was noticed in the suffered from burns in general. The levels of IgG and IgA tended to be normal after the treatments with the water extract from the studied plants and with the organic acids.o The L D50 of N. sativa and E. sativa was 18 mg/kg while it was 16 mg/Kg of L. usitatissimum.

دراسة جزيئية عن جين المقاومة mecA في بكتريا العنقودية الذهبية المقاومة للمشيسلين والمعزولة من بعض مستشفيات بغداد == Molecular Study For Detection of Meca Gene In Methicillin - Resistant Staphylococcus Aureus Isolated From Some Hospital In Baghdad City

اسم المؤلف: لمى ياسين موسى
اسم المشرف: محمد ابراهيم نادر
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two hundred and thirty clinical specimens were collected from two different hospitals in Baghdad during the period (December 2012 to April 2013). These specimens were collected from visitors, hospitalized patients and the health care workers in these hospitals. The specimens included nasal swab, wound swab, burn swab, abscess and pus, sputum, ear swab, urine and blood culture diagnostic results show that 150 out of the (230) specimens gave positive bacterial cultures and (100) isolates are characterized as Staphylococcus aureus depending on the cultural and biochemical examinations.the coagulase test was performed and the results showed that from total 150 isolates of Staphylococci, 100 isolates (61%) were coagulase - positive (COPS), while only 50 isolates (39%) were coagulase negative (CONS). In addition, the distribution of methicillin resistance among Staphylococcus spp. was investigated.The use of the antibiotic susceptibility profile for these isolates was examined against methicillin resistance. Using disk diffusion method revealed that (13) isolates were proved to be methicillin resistant Staphylococcus aureus (MRSA), While (87) isolates of S. aureus showed sensitivity to methicillin (MSSA) and there was no intermediate resistance among these isolates.The ability of MRSA isolates to produce some virulence factors were investigated and the results showed that MRSA isolates produce many enzymes and toxins that contributed in their virulence such as protease, urease, dnase and gelatinase, and also produce a beta types of haemolysins.The ability to produce slime layer by MRSA isolates was also investigated and the results showed that all isolates of MRSA were produced slime layer when theytested by Congo red agar method and the results showed that all of MRSA isolates produced strong slime layer.One of the aims of this study was to adopted a accurate diagnostic method to detect S.aureus by its genetic material contents through extracting of DNA and gel electrophoresis of the PCR product for the specific gene.Detection of methicilline - resistance gene represented by A confirmatory test was carried out for the selected isolates using Polymerase chain reaction (PCR) technique for further characterization up to the species level by the amplification of (mecA) gene.This is Staphylococcus aureus specific gene that encodes the extra Penicillin Binding Protein, which is unique to methicillin - resistant staphylococci. All the (13) positive isolates by disk diffusion test are found to be positive for the presence of (mecA) gene as their agarose gel revealed the presence of DNA band of mecA gene with a molecular size about (200 bp.).Results of the detecting (femB) gene showed that it was positive in all of MRSA isolates as they appear to have a band with a molecular size of about (651 bp). The genetic determinants of methicillin resistance mecA and femB genes were amplified using multiplex PCR technique in order to identify methicillin resistant (mecA+) and susceptible (lacking mecA) staphylococci and to differentiate S. aureus (femB+) from coagulase negative staphylococci (lacking femB). All of the S. aureus isolates (100%) were found to harbor femA gene, it is species specific marker for S. aureus.

استجابة ثلاثة اصـناف من حنطة الخبز Triticum aestivum L. باضافة سماد NPKZn والرش بالجبرلين == Response of Three Bread Wheat (Triticum Aestivum L.) 3With Gasprying Fertilizer And NPKZn Cultivars To

اسم المؤلف: عبد الجبار جاسم جريان
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A field study was conducted at the farm of Biological Dpartment, College of Education (Ibn Al - Haitham) University of Baghdad during the grown season 2013 - 2014, to study the effect of compound fertilizer NPKZn (15 : 15 : 15 : 1) - Turkish origin was introduced to Iraq recently - in both ) 3(GA acid Gibberellicvarious concentrations of with morphological and chemical indicators, the components of yield, some quality and biological characteristics of three varieties of the Iraqi wheat (Bohoth 22, Alfath, Bohoth158). Experiment was carried out according to the design of complete sectors of randomization [Randomized Complete Block Design (RCBD)].160) (0 of fertilizer, two levels used experiment Asthree replicates, with ) 015, 100, acid concentrations (25, 50 Gibberellicof fourand 1 - .hKg and three varieties of as well as to the treatment of comparison 1 - .lmg wheat. So the expriement was factorial on (2 x 5 x 3).It included 90 experimental units..The study of components of yield have gone on its : 1. There is difference in these three different varieties Bohoth 22, Alfath, and Bohoth 158 in the studied indicators.The class Bohoth 158 exceeds on the other categories in the following phenotypic indicators (dry weight of vegetative part, space of flag leaf and sustainability of biomass), with increase as like {(17.85, 11.37), (14.66, 7.33) and (17.02, 14.24)}%, respectively, while Bohoth 158 significantly exceeds on the other varieties absolute growth rate. And also the differences were significant between varieties in the following chemical indicators (phosphorus and calcium content and the percentage of protein in the grain) and in the components of yield there was significant increase in the (spike length with awn and without its, the number of spikes, weight of spike and its grains, weight of grains in spike and grains yield), while in the Bohoth 158 there was a significant increase in qualitative and rheological characteristics for the following qualities, (the proportion of wet and dry Glutine, flour absorption susceptibility of water, sedimentation values, ash content in coarse bran compared to others two classes. 2. The results of the study showed that the transactions under the influence of fertilizer has excelled significantly in all the study indicators (morphological and chemical components of yield, quality and rheological) with the exception of the dry weight of roots. acid was the best among Gibberellic 1 - .lmgof 100 concentration 3. The the concentrations giving him the highest average of indicators of morphological and physiological components of yield, quality and rheological. and spray 1 - 160 Kg.hbetween the manure level overlap 4. The result Gibberelllic acid showed 1 - mg.l 100 of concentration under the significant increase in all studied indicators except for the (dry weight of roots, the sustainability of biomass, and 1000 - grain weight) 5.The trio overlap transactions showed exceeding of Bohoth 158 nder the influence u 1 - .hKg160 in level fertilizer of add ithw significantly 22 Bohothcategories : other acid onGibberellic of 1 - .lmgof spraying 100 and Alfath in the following indicators : Dry weightof the flag leaf, spike length with awn, number of spikes, grains yield, the percentage of dry Glutine and the values of sedimentation.

دراسة بيئية للدايتومات الملتصقة على بعض النباتات المائية في هور العودة ضمن محافظة ميسان جنوب العراق == A Study On The Ecology of Epiphytic Diatom On Some Aquatic Plants In Al - Auda Marshes / Maysan Province / Southern Iraq

اسم المؤلف: الاء عيسى موسى البوعجي
اسم المشرف: جنان شاوي الحساني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الفصلية على الدايتومات الملتصقة على النباتات المائية لثلاث مواقع في هور العودة ضمن محافظة ميسان جنوب العراق (من شهر تشرين الاول 2013 لغاية شهر تموز 2014) نظرا لاهمية الدايتومات في الانظمة البيئية المائية والاهمية البيئية لاهوار العراق على مس | Seasonal study on the quality and quantity of epiphytic Diatoms was conducted in the Al - Auda marsh within Maysan Province throughout one year from October 2013 to July 2014.These Diatoms have an important role in biotic ecosystems. Three taxa of aquatic plants were selected (Phragmites australis Trin ex stand, Ceratophyllum demersum L. and Typha domengensis Pers) in three sites within Al - Auda Marsh; these sites were Al - Adleh, Al - Auda and Um - Almashahef. Also, the study included measuring physical and chemical factors of all the study sites, such as : temperature (air and water), power of hydrogen (pH), total hardness(TH), Calcium (Ca), Magnesium (Mg), turbidity (Tur.), light penetration(LP), depth of water (D), total dissolved suspend material (TDS), total suspended solids (TSS), total alkalinity (TA), dissolved oxygen (DO), total nitrogen (TN) and total phosphorus (TP) and reactive Silicate (SiO3). The mean ranges of the studied factors were recorded : air temperature (10 - 38 C°), water temperature (13 - 34 C°), pH (7.1 - 8.6), TH (672 - 3800mg/l), Ca (97.8 - 264.5 mg/l), Mg (125.1 - 896.13mg/l), Tur.(7.62 - 30.7NTU), LP(46 - 113cm), D (190 - 413cm), TDS (1170 - 3075 mg/l), TSS (0.02 - 0.08 mg/l). while TA (207.5 - 300mg/l), DO (6.4 - 13.5 mg/l), TN (2.41 - 8.58µg/l), TP (0.013 - 0.23 µg/l) and SiO3(101 - 776.77 µg/l).Results of the study indicated presence of 111 taxa of epiphytic diatoms, which belonged to 13 families and 26 genus (one family and two genus of centric diatoms, 12 families and 26 genus of pennate diatoms).The present study recorded 21 species that were found only on one host plant, 20 species that were found on two host plants and 70 species that were found on all host plants, nine of diatoms taxa were present in all host plants among all study seasons which are Achnanthes minutissima, Cocconeis placentula var. euglypta, Cyclotella meneghiniana, Synedra affinis, Syneda ulna , Gomphonem parvulum, Mastogloia smithii var. amphicephala, E. zebra var. porcellus, Rhopalodia gibba.One of the diatoms taxa was recorded in the present study is new to Iraqi flora, the new record of diatom include one taxa of Achnanthidiaceae family (Achnanthes exigua var. constricta). The results of this study included description of the basic characteristics of this taxa with complete photographic documentation.It was evident, from the results, that the morphological form of host plants influenced the number of the attached epiphytic diatoms. C. demersum had attracted 92 diatoms taxa(35%) while the lowest number was 82 taxa (31%) attached by Typha domengensis. The seasonal variation of diatoms growth was evident. Spring 2014 was characterized by the highest number of attached taxa (69 on P. australis - 11%) whereas the lowest number was observed on C. demersum (32 taxa - 5.4%) in autumn 2013.Numbers of epiphytic diatoms fluctuated among study sites, seasons and aquatic plants. The total number of epiphytic diatoms on P. australis ranged between 0.73×104 cell/gm wet weight in summer 2014 at Al - Auda and 406.89104 cell/gm wet weight in spring 2014 at Um - Almashahef. C. demersum recorded highest total number 513.9 ×104 cell/gm wet weight in winter2014 at Al - Adleh, while the lowest total number was 0.1×104 cell/gm of wet weight was recorded in the spring 2014 at Um - Almashahef. While total number of taxa on T. domengensis ranged (0.84 - 427.89) ×104 cell/gm wet weight in summer and winter 2014 at Um - Almashahef.Fluctuations in the total number of main epiphytic diatoms families were observed on all aquatic host plants throughout the study period. The higher total number(92.59×104 cell/gm of wet weight) of diatoms recorded in Achnanthidiaceae family in summer 2014 on P. australis and lower total number (0.28×104 cell/gm of wet weight) on same plant observed in Rhoicospheniaceae family in spring 2014. C. demersum showed highest total number (95.15 ×104 cell/gm of wet weight) in Fragilariaceae family in spring2014, while the lower total number (0.3×104 cell/gm of wet weight) was in family Rhoicospheniaceae in autumn 2013. Individual of Achnanthidiaceae family were recorded as highest total number (168.57×104 cell/gm of wet weight) on T. domengensis in Achnanthidiaceae family in the winter of 2014, but lower total number (0.28×104 cell/gm of wet weight) recorded in Coscinodiscaceae family in the same season. The study showed highest values for richness index (3.77) in the spring of 2014 for epiphytic diatom on C. demersum in Al - Auda site, while lower richness index recorded was (0.6) on T. domengensis in theautumn of 2013 at Um - Almashahef. Shannon - Weaver index ranged (0.24 - 2.45) for epiphytic diatom on P. australis during winter of 2014 at Um - Almashahef and autamn 2013 at Al - Auda, respectively. Highest values for Evenness index were recorded (0.28) for epiphytic diatom on C. demersum in the spring of 2014 at Um - Almashahef site, while for the same site lower values recorded was (0.03) on T. domengensis in winter 2014. Higher percentage for Jaccard similarity index (63.2%) obtained between epiphytic diatoms hosted on T. domengensis and lower percentage of similarity (28.9%) found among the common taxa of epiphytic diatoms on C. demersum, and highest percentage for Jaccard similarity index between host plants was (70%) between P. australis and C. demersum while lower percentage of similarity(67%) was obtained between T. domengensis and C. demersum.

دور الكاينيتين والسماد المركب (NPKZn) في تحمل نبات الفلفل Capsicum annuum L.)) للاجهاد الملحي == Role of The Kinetin And NPKZn In Salt Stress Tolerance of Sweet Pepper (Capsicum Annuum L.)

اسم المؤلف: سعاد عبد سيد الجلالي
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجربة اصص في الحديقة النباتية التابعة لقسم علوم الحياة، كلية التربية للعلوم الصرفة - ابن الهيثم، جامعة بغداد في الموسم الربيعي عام 2014 هدف الدراسة كان دراسة تاثير تركيزين من كلوريد الصوديوم هي 50) و100) مليمول.لتر - 1 فضلا عن معاملة السيطرة واربعة | The aim of the pots experiment which was conducted in the Botanical Garden of Biology Department in the Faculty of Education /Ibn - Al - Haytham / University of Baghdad, during the spring season of 2014 was the influence of different concentrations of Sodium chloride (50, 100) mM.L - 1 in addition to the control treatment and and Four concentrations of kinetin (25, 50, 75, 100) ppm in addition to the control treatment.It is also aiming to study the effect of application and non application the level 160 Kg.H - 1of NPKZn fertilizer, and their interactions in some morphological characteristics root length, root size, plant height, the internode length, stem diameter, Leaf area, dry weight for root and shoot and the absolute growth rate for shoot.and some physiological characteristics Elements content and protein ratio in root, Elements content, protein ratio, total chlorophyll content, carbohydrates percentage and cytokinine concentration in shoot. Biochemical characteristics (enzyme activity of the enzymes) (SOD, POD and CAT) and some antioxidants that nonenzymatic : (the proline and vitamin C concentration) in shoot. Malondialdehyde (MDA) content in shoot, and some Floral morphological, physiological and biochemical characteristics of fruits (Number of branches, The total number of flowers, Number of blooming flowers, ratio of aborted Flowers, Number of knotted flowers, the number and size of fruits, their wet weight , nitrogen and calcium concentration, protein percentage and vitamin C concentration in fruits of pepper plant California weleander variety.The experiment was designed according to Randomized Completely Block Design (RCBD)with three facters kinetin, manure addition and Sodium chloride,) consisting 90 pots. Seedling of pepper plant was planted on 4/2/2014 acompanied by making all agricultural operations of irrigation and the removal of the jungles. results were obtained can be summarized as follows : 1 - The results showed that increasing the concentration of sodium chloride from zero to 100 mM.L - 1 Led to a decline in morphological, physiological and floral characteristics, with and significant increases in the content average of sodium, chloride, The proportion of flowers aborted and biochemical characteristics. The rate quality effectiveness for enzyme SOD increased from 7.24 to 34.66, POD from 8.10 to 26.35 and CAT from 4.91to 39.57 (unite mg protein - 1) , and proline acid content from 23.05 to 40.30 (mg.L - 1) and vitamin C concentration from 2.67 to 3.68 % and Malondialdihyde (MDA) from 1.84 to 3.86 (µM.gm F.W. - 1)2 - As concerns kinetine treatment effect, the results showed a significant increase in the average of morphological, physiological and floral characteristics, and significant decreases in the average of sodium, chloride contents and biochemical characteristics, in the cased increasing in the concentration of kinetine from 0 to 100 ppm caused decreases in the average of quality effectiveness for enzyme SOD from 25.79 to 21.91, POD from 21.83 to 17.20 and CAT from 27.88 to 21.73 (unit mg protein - 1) , and proline acid content from 34.27 to 31.68 (mg.L - 1), Malondialdihyde (MDA) from 3.15 to 2.85 (µM.gm F.W. - 1) and vitamin C concentration from 3.42 to 3.23 %.3 - application the level 160 Kg.H - 1of NPKZn Manure caused a significant increases in all averages morphological, physiological and floral characteristics, and significant decreases in the average of sodium and chloride contents and biochemical characteristics, fertilizing with the level 160 Kg.H - 1of NPKZn fertilizer caused decreases in the average of quality effectiveness for enzyme SOD from 22.03 to 19.88, POD from 17.70 to 14.53 and CAT from 23.34 to 17.34 (unite mg protein - 1) , and proline acid content from 32.84 to 28.93 (mg.L - 1), Malondialdihyde (MDA) from 2.97 to 2. 75 (µM.gm F.W. - 1) and vitamin C concentration from 3.29 to 3.03 %.4 - Results of the bilateral interpenetration between the Fertilization by fertilizer NPKZn and Sodium chloride concentrations, showed that the fertilizations has a positive and effective influence in reducing the negative effects of sodium chloride in all characteristics studied. 5 - The results showed the positive role of kinetine in decreasing the negative effects of sodium chloride Through bilateral overlap between factors. Especially when the concentration of 75 ppm of kinetine and concentration 100 of sodium chloride In all morphological, physiological and biochemical floral characteristics of plant.6 - The results indicated that the Interference between the fertilizer NPKZn and kinetine has a positive and clear role to both factors increase morphological, physiological, floral characteristics and reduce biochemical characteristics.7 - The effect of the triple overlap between study factors was significant, The results showed That plants sprayed kinetine And fertilization led to a significant reduction of most of the negative effects resulting from the of high concentrations of Sodium chloride in all morphological, physiological and flowering traits. Especially in the concentrations 75 ppm kinetine and in the existence of fertilization.

استخدام الزراعة النسيجية والفطر Glomusmossaes في الازالة الحيوية لعنصري الرصاص والكادميوم وتحمل الملوحة لنبات السيسبان Sesbaniarostrata L == Application of Plant Tissue Culture And Glomusmossaes In Bioremediation of Lead, Cadimum And Salinity Using Sesbaniarostrata L. Plant

اسم المؤلف: التفات فاضل شحاذة الطائي
اسم المشرف: علي هاشم الموسوي | كاظم محمد ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجارب عدة لغرض دراسة تحمل نبات السيسبان Sesbania rostrata L. للكادميوم والرصاص وكلوريد الصوديوم على مستوى النبات الكامل ومزارعة النسيجية. استحث الكالس واديم على وسط موراشيج وسكوج 1962 والمجهز 0.3 ملغم/لتر من الكاينتين و2 ملغم/لتر من حامض 2, 4 داي مث | Several experiments were carried out to study cadmium, lead and sodium chloride tolerance at the tissue culture or whole plant levels of Sesbania rostrata. Callus was induced and maintained on Murashige and Skooge (1962) medium (MS) supplemented with 0.3 mg/l Kinetin and 2 mg/l 2, 4 - dichlorophenoxy acetic acid (2, 4 - D) using cotyledons as the source for callus induction. Different concentrations of cadmium, lead and sodium chloride directly or gradually were added to the culture medium as contaminants. Selected tolerant cell lines were subjected to regeneration. Callus showed better tolerance to gradual exposure than direct addition. The concentrations of Potassium, lead, Cadmium and Sodium recorded to 17, 24, 27, 35 ppm when 1.0, 2.0 mg/L and 0.4% of cadmium, lead and sodium chloride were added respectively. The study included the effect of the cytokinin (benzyl adenine, BA) and the auxin (Naphthalene acetic acid, NAA) on the regeneration of shoots from callus tolerant to pollutants reached 76, 87 and 85% for callus tolerant to Cadmium, Lead and sodium chloride respectively, when the combination of 0.7 mg/l BA and 0.2 mg /l NAA was supplemented to the medium. The effect of indole butyric acid (IBA) on rooting of shoots. The concentration 0.7 mg /l gave highest percentage of rooting amounted to 66, 70 and 85% in a medium supplemented with cadmium, lead and sodium chloride respectively. Acclimatization of plantlets using river sand soil recorded 85% survival. The study was also included effects on plant height and fresh weights, shoot fresh weight was superior at the concentration 1.0 and 2.0 mg/l of cd and pb recording 8.7 and 7.5 g respectively. Treatment with NaCl led to increase the root fresh weight recording 19.1 g. Maximum cd accumulation reached 12.5 ppm in shoots, 10.5 ppm for lead in roots, 39 ppm for NaCl in roots. The experimental work investigated the effect of mycorrhiza (Glomus mosseae) on cd, pb and NaCl removal by Sesbania plant when grown in either soil contaminated with cd, pb and NaCl or non - contaminated soil (control). Inoculation with mycorrhiza led to an increase in seed germination percentage (96%), plant height (28 cm) in non - contaminated soil, while achieved an increase in shoot and root fresh weight recording 10.0 and 6.8 g respectively. Inoculation with mycorrhiza also led to a significant increase in cd and pb accumulation in roots reached 9.0 and 87.4 ppm respectively. Meanwhile, Mycorrhiza caused a significant increase in Na accumulation in root system (42.5 ppm) which already affected K concentration as a result of competition with Na recording 16.5 ppm in shoots. Mycorrhiza inoculation led to a significant increase in number of root nodes in non - contaminated soil recording 86%. Finally, mycorrhiza succeded in infecting Sesbania rostrata roots reached 90% in non - contaminated soil while it was 85% in contaminated soil

دراسة وراثية جزيئية لبكتريا المكورات المعوية البرازية Enterococcus faecalis المعزولة من جذور الاسنان الملتهبة للانسان في بغداد == Molecular Genetics Study of Enterococcus Faecalis Isolated From Root Canal Infection of Human In Bagdad

اسم المؤلف: سوزان علي كاظم
اسم المشرف: عذراء حميد حسون
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم التحري عن نسبة وجود بكتريا المكورات المعوية البرازية Enterococcus faecalis في (100) عينة معزولة من اشخاص مصابين بالتهاب قناة جذر السن, اذ تم جمع (70) عينة من الاصابات الابتدائية لقناة الجذر، و(30) عينة من الاصابات الثانوية لقناة الجذر (اعادة العلاج) وم | To detection Enterococcus faecalis in (100) root canal sample were collected from primary and secondary root canal infection patient`s from all the ages (10 - 50) during the period of (August 2013) till (January 2014). Detection depending on cultural & microscobial characteristics of bacterial cell was done to find (45) 0f E. faecalis species & Biochemical tests & Serological diagnosis by Lancefield method done to find (24) isolates of this species & Diagnosis by Vitek2 was done to find (20) isolates of E. faecalis. When the Molecular genetics Diagnosis was done the result showed find (32) isolates belong to E. faecalis. The antibiotic sensitivity test was done by using (14) antibiotics, (5) isolates showed resistant against all antibiotics & the isolates showed multiresistant against for some antibiotics. All the isolates were resistant by (100 %) against (5) antibiotics. To detection of the isolates ability of production of protease enzyme, lipase enzyme, hemolysin enzyme & gelatinase enzyme.The results showed that (24 isolate) (75 %) were protease producer, & (8 isolates) (25 %) were lipase producer, & (16 isolate) (50 %) were hemolysin producer, & (5 isolates) (15.6 %) were gelatin producer. To detection the presence efa A gene of the isolates by used specific primer to this gene, and all isolates

دراسة تصنيفية مظهرية خارجية لبعض انواع عائلة الفراشات فرشاتية الاقدام من رتبة حرشفية الاجنحة (Lepidoptera : Nymphalidae) في وسط وشمال العراق == Morphological Taxonomic Study of Some Species of Brush Footed Butterflies (Lepidoptera : Nymphalidae) In Mid And North of Iraq

اسم المؤلف: ايناس صادق عبد الكريم
اسم المشرف: عماد احمد محمود | حسن سعيد الاسدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study comprises taxonomic morphological study of seven species belonging to four genera of the subfamiliesNymphalinae, Danainae, Satyrinae belong to the family Nymphalidae of the order Lepidoptera.The following species were studied in the subject are : Famliy : Nymphalidae Swainson, 1827 - Subfamily : Nymphalinae Swainson, 1827Genus : Vanessa Fabricius, 1807Vanessa japonica Linnaeus, 1758Vanessa cardui Linnaeus, 1758Vanessa atalanta Linnaeus, 1758Genus : Junonia Hubner, 1819Junonia orythia Linnaeus, 1758 - Subfamily : Danainae Swainson, 1827Genus : Danaius Kluk, 1802Danaius chrysippusLinnaeus, 1758 - Subfamily : Satyrinae Boisduval, 1833Genus : Coenonmphyma Hubner, 1819Coenonmphyma pamphilus Linnaeus, 1758 Coenonmphyma spThe species Vanessa japonicaLinnaeus added as new record to the Iraq insect fauna of Lepidopteraand the species Coenonmphyma sp added as new expected species to the world.

العلاقة بين انتاج الانزيم المحلل للكولاجين وتكوين الغشاء الحياتي بوساطة بكتريا Pseudomonas aeruginosae == The Relationship Between Collagenase Production And Biofilm Formation By Pseudomonas Aeuroginosa

اسم المؤلف: امال عزيز كريم السعدي
اسم المشرف: شذى سلمان الطحان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A total of 359 samples divided as 228 clinical and 131 non clinical specimens were collected during 2012 from four hospitals in Baghdad city including : Al - Kadhymia Teaching hospital, Baghdad Teaching hospital, The Burn Specialist Hospital and Al - Imam Ali hospital, for isolation of P.aeruginosa to study the correlation between collagenase production and biofilm formation. Eighty two Pseudomonas isolates were screened for biofilm formation, 28 isolates were classified as strong biofilm formers, 25 as moderate and 27 as weak biofilm former. The 28 isolates were identifid by VITEk - 2 Compact system which confirmed that the isolates were P.aeruginosa. Collagenase production assay was used to screen 28 isolates that were strong biofilm formers inorder to detect the ability of these isolates to produce collagenase, the substrate of collagenase (collagen) was purified localy from bovin tendon and the results showed that just 8 isolates could grow in mineral salt media with collagen after 4 days of incubation. The factors affecting biofilm formation and collagenase production were studied to determine the optimual conditions for their production, those factors included : 1 - Nitrogen sources represented higher influence on collagenase production specialy (yeast extract) in media containing collagen than other media without collagen as a substrate. The specific activity differed between the 8 isolates, biofilm formation also became more pronounced with (yeast extract), while NH4Cl and NaNO3 depressed biofilm formation at the same conditions. The statistical analysis between the two parameters (biofilm and collagenase) according to different nitrogen sources demonstrated highly significance at p?0.01 with yeast extract and casein. 2 - pH, results showed that the best pH for production was 7 for both collagenase and biofilm.The statistical analysis for determination the relationshipe between the two parameters showed highly significance at p ?0.01 for different pH. 3 - The maximum production of the two parameters was at 35?C temperature which gave highly significance at p?0.01 with defferent temperature. 4 - Long incubation periods revealed increasing in collagenase production and biofilm formation which represented highly significance detween them when incubation periods were prolonged at p?0.01. Results of this study showed that collagenase production increases when bacteria switch from a planktonic to biofilm phenotype. This indicates that biofilms and collagenase are more virulent and have a greater ability to cause tissue destruction. The REP - PCR analysis using BOX - primer, showed a clusters genetic relatedness among the isolates. The isolates were grouped according to the REP - PCR in 9 different genotypes, named cluster 1 to 3 which included C1, C2, C3 with relatedness : 8 (80%), 8 (86%), 3 (80%) respectively. A19 and A20 both of them were not included in any cluster, they have 78% similarity.The REP - PCR analysis showed that the genotypic relatedness is consistently high between the 8 producer isolates and non producer isolates (13), showed similarity reached 86

عزل وتشخيص الجين lipA المنتج من بكتريا الزائفة الزنجارية من مياه الصرف الصناعي == Isolation And Identification of lipA Gene Producing Pseudomonas Aeruginosa From Industrial Wastewater

اسم المؤلف: انتصار طه لفتة
اسم المشرف: واثق عباس الدراغي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: توضح هذه الدراسة عزل lipA gene من الزوائف الزنجارية من مياه الصرف الصناعية للزيوت النباتية وتشخيصها اعتمادا على تفاعل البلمرة المتسلسل (PCR). استغرقت هذه الدراسة تسعة اشهر, من شهر شرين الاول 2014 لغاية حزيران 2015. تم جمع خمسين عينة من مياه الصرف الصناع | This study clarify the isolation and identification of lipA gene in Pseudomonas aeruginosa from industerial wastewater of vegetable oils factories depending on the polymerase chain reaction (PCR) technique. The present study had taken nine monthes starting from October 2014, till the mid of June 2015. Fifty samples of industrial wastewater were collected from the factories of the general company of vegetable oils, fourty from AL Rasheed factory and ten from AL ameen factory, the samples collected from the physical, chemical and biological treatment units, and other different wastewater tanks departments. While the four sewage samples were collected from sewer service Baghdad /Alrustumaiya. For Identification of Pseudomonas aeruginosa, two types of tests were used in this study. The first type was the routine tests, which include selective cultures, bacteriological and biochemical tests. Thirty four of fifty samples (68%) gave a positive growth and results for tests that were used to confirm the presence of P. aeruginosa. In addition to diagnosis P. aeruginosa in sewage sample which prepare the biological treatment units in vegetable oils factories as active sludge, other bacterial types have been diagnosed by using traditional methods and API 20 E system. For detection of bacterial lipolytic activity, two methods were used for this purpose, the first was the screening of bacterial lipolytic activity which was based on values of clear zones diameter around bacterial colony. The best values were between (1.9 _ 2.7) cm. The second test was carried by the Gas - Chromatography - Flame ionization detector, fatty acids solutions that were produced from hydrolysis by lipase enzyme were extracted by Petrolium ether solvent then analyzed by gas chromatograph apparatus. The second type of test was the Molecular diagnosis by using the Polymerase Chain Reaction (PCR) to detect lipA gene in Pseudomonas aeruginosa by two primers. Through two primers used, lipA 948 was the best and more specialized primer to isolation lipA gene of P. aeruginosa. It gave (100%) a positive result. While the second primer lipA 558, gave (66.66%) a positive result and that may be due to the design of this oligonucleotides was not specific for lipA of P.aeruginosa. This is so as was based on highly preserved region of 12 bacterial lipA - homologous genes for many genus and many species belong to Pseudomonas. DNA sequencing done for amplicon generated using lipA 948, this sequence aligned by using BLASTn software against NCBI database to validate the results and investigate the similarity degree with other corresponding strains.

الاكثار الخضري لنبات Spilanthes acmella (L.) Murr. خارج الجسم الحي == In Vitro Vegetative Propagation of Spilanthes Acmella (L.) Murr

اسم المؤلف: انسام زهير جاسم الحسني
اسم المشرف: بشرى محمد جابر علوش
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: وظفت تقانة زراعة الانسجة لاكثار نبات Spilanthes acmella (L.) Murr. كونه نباتا غير مستزرع في العراق ولاهميته الطبية والزراعية بوصفه نبات زينة فضلا عن استعمالاته الاخرى. عقمت البذور بمادة هايبوكلورات الصوديوم(NaOCl) وزرعت على وسط Murashige وSkoog (1962) (MS | The technique of plant tissue culture has been manipulated to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized by sodium hypochlorite (NaOCl) and cultuared on full strength Murashige and Skoog medium(1962) (MS). Nodal segments, apical shoots, and leaflets explants were taken from seedlings and cultured on (MS) medium enriched with different concentrations of Indol acetic acid (IAA), Naphthalene acetic acid (NAA) Benzyl adenine (BA), and 6 - furfurylaminopurine (Kin.). The study include the effect of node orientation vertically and horizontally on induction of shoots formation. The effect of the interaction between IAA and BA, IAA and Kin. on shoot multiplication was investigated. Rooting was also studied after inclusion of IBA and IAA to full and half - strength (MS) medium. At acclimatization stage, different ratios of river sand and peat moss as agricultural media were tested and plantlets survival was recorded. DNA isolation was carried out from fresh and dry plantlet leaves as well as from seeds and Agarose Gel Electrophoresis (AGE) was done. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. The nodes cultured vertically exhibited relatively highest response as compared with apical shoots and leaflets culture. Supplying the culture medium with 1 mg/L. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding IAA at 0.1 and Kin. at 0.5, 1.0 or 1.5 mg/L. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 14.20, 13.60 shoots/ explants with a mean length of 3.40, 2.60, 1.40 cm, respectively. Adding 0.1, 0.5, 1.0 mg/L IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots, respectively, with mean length of 3.25, 3.80, 3.80 cm, respectively. Plantlets were acclimatized successfully achieving 100% survival after four weeks when transferred to agricultural medium consisted of river sand and peat moss at 1 : 1 v/v ratio. This study showed the ability of in vitro micropropagation of Spilanthes acmella (L.) Murr. DNA isolation from drying leaves was butter than fresh leaves with the precense of liquid nitrogen.

تاثير الكلوتاثيون وبيروكسيد الهيدروجين وتداخلهما في بعض الصفات النوعية والكمية لنبات الماش Vigna radiat == Effect of Glutathione And Hydrogen Peroxide And Their Interactions On Some of The Quantity And Quality Characteristic of (Mung Bean) Plant Vigna Radiata L.

اسم المؤلف: ايمان حسين هادي الحياني
اسم المشرف: وفاق امجد القيسي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments were conducted during the spring and autum growth season of Vigna radiata L. in the year 2014 at botanical garden of Biology Department, Collage of Education for Pure Science (Ibn AL_Haithem) University of Baghdad.The experiments aimed to study the effect of glutathione and hydrogen peroxide, and their interactions on some of quantity and quality characteristic of plant Vigna radiate L.The treatment of glutathione (0, 25, 50, 75, 100) mg.L - 1 While the H2O2 (0, 5, 10, 15) ml mol. L - 1.The two experiment were designed as Randomized Complete Block Design as factorial experiment with two factors with three replication which included 60 experiment units the area of each one (1×1`) m. Results were analyzed Statistically and compared using average L.S.D at 0.05. The results of the experiments showed that glutathione revealed significantly increased all study parameter espically in 100 mg.L - 1 concentration, stem diameter by 54.56%, 28.04%, leaves number 69.34%, 53.14% for two seasons, fresh weight 44.20% for first season, the dry weight 37.43%, 91.46%, leaf area 61.61%, 151.23%.leaf area index 86.61%, 146.35%, biomass duration 60.48%, 101.06%, absolute growth rate (AGR) 32%, 92.68%, inflorescences number 56.55%, 39.90%, flower`s number 71.97%, 22.60%, root length19.82. %, 26.20%, root dry weight 76.10%, 79.25%, total function of SOD 69.32%, 40.52%, total Function of POD 29.45%, 82.25%, total Function of GPX 30.90 %, 63.62%, chlorophyll concentration of a 73.48%91.40%, chlorophyll concentration of b 35.42%, 17.67% for two seasons respectively. The total chlorophyll content 13.69%, for second season, Caroten concentration 207%, 309%, proline concentration 84.47%, 31.75%, (MDA) concentration 6.25%, 38.35%, glutathione. concentration 41.49%, 23.62 %, H2O2 concentration 52.16%, 33.24%, pods number 17.43%, 16.93%, 100 seeds weight 22.95 % 22.48%, , 93.62%, seeds yield 52.17%, 43.70%, the carbohydrate percentage 64.07%, 19.21 %, the protein percentage 22.32% for first season while the results of effect of soaking seeds with H2O2 specially with 15m ml.L - 1 concentration increased stem diameter 45.52%, 34.00%, leaves number 18.55%, 30.75%, Lateral branch 26.06%, 48.04% for Two seasons respectially, fresh weight 35.45% for first season, dry weight 40.89%, 43.85%, leaf area 53.24%, 64.53%, leaf area index 92.30%, 62.39%, biomass duration 35.35%, 57.53%, abosult growth rate34.78 %, 48%, in, inflorescences number 34.98%, 38.10% flowers number 2.987, 20.13%, root length19.44 %, 19.36%, root dry weight 99%, 96.52% total functhion of (SOD)100%, 2853%, total function of (POD) 176.57%, 40.58%, total function of (CAT)118.29%, 71.78%total functhion of (GPX)12.80%, 61.40%, Caroten concentration 54%for second season, prolineconcentration 40.93%for first season, (MDA) content 17.31% for second season, glutathione concentration, 13.68% 24.29%, H2O2 concentration 26.53%, 30.58%, number of pods 42.77%, 20.51%, seads number /pod 22.93%, 22.93%, 100 seeds Wieght 24.07%, 24.86%, seeds yield 16.69% for first season, the solubly carbohydraty percentage 43.26%for first season, the protein percentage 11.50%, 25.18 % for two seasons respicatlly, while chlorophyll a decreas concentration ed by 15m ml.L1 12.87%for second season, total chlorophyll concentration 39.66%, 26.22 for two season respicatlly. There were high significant interaction between glutathione and H2O2 in all study parameter characters in the two experiments.

تاثير الجسيمات النانوية للنحاس على بعض اعضاء الجهاز التكاثري الذكري للفئران البيض Mus musculus == Effect of Copper Nanoparticles On Some Organs of Male Reproductive System In Albino Mice Mus Musculus

اسم المؤلف: ايمن راضي حبيب
اسم المشرف: محمد ناجي طه
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study was conducted on 20 male of Swiss albino mice, where the main target of this study was to identify the influence of copper nanoparticles (Cu - NPs) on some organs of the male reproductive system. Animals were orally administered with 0.1 ml of 100 mg/kg Cu - NPs for 7, 14, 21 days. Then the animals were dissected in the next day after the end of the dosing period and took from them testes and epididymis (head and tail) to study the parameters in which these particles have affected, and this parameter as follows : 1 - Changes in the weights of each of the animals bodies, testes, tunica albuginea, and epididymis (heads and tails). 2 - Changes in sperm characteristics in testes and epididymis (head and tail), which included : the percentage of live sperms, abnormal sperms and sperm concentration. 3 - Changes in the percentages of the spermatogenic cells, which included : a - Spermatogonia. b - Primary & Secondary Spermatocytes. c - Spermatids. d - Sperms. 4 - Changes in numbers of leydig cells and nuclei diameters. 5 - Changes in diameters of seminiferous tubules and their lining wall thickness. 6 - Changes in thickness and height of epithelial cells in both the head and tail of the epididymis. 7 - Histopathological changes in testes and epididymis (head and tail). Phenotype description of testes from mice treated with copper nanoparticles for a period of 21 days occurrence of congestion and a few amount of fatty material around the testes. The results also revealed a decline in the average body weights on the period of 7 and 21 days of treatment when compared with the weight of the same animals before treatment and the decline was significantly at (P<0.05). Whereas the testes and tunica albuginea weights recorded a significant decrease in their average weights (P<0.05) when compared with control groups. As well as the epididymis weights where showed significantly decrease (P<0.05) in the average of the epididymides heads weights for all three periods of treatment, while a significant decrease (P<0.05) in the average weight of the epididymis tail appear for two periods 7 and 21 days compared with control groups. The study also reported a decrease in the percentage of live sperm and the average sperm concentration and an increase in the percentage of sperm abnormalities extended to all periods of treatment compared with control groups and this change was significant at the level of (P<0.05). The number of leydig cells and nuclei diameters, the results show a decrease in their averages and all the extended treatment period when compared with control groups, and this decrease was significantly at (P<0.05). Treatment with particles of copper nanoparticle indicated a decrease in the percentage of spermatogonia, primary spermatocytes and sperms in all periods of treatment compared with control groups and this decrease was significantly at the level of (P<0.05). While the results of the study revealed a significant increase at the level of (P<0.05) in the percentage of secondary spermatocytes and spermatids compared with the control animals. The study also showed a significant decrease at the level of (P<0.05) in the diameter of the seminiferous tubules of testes and the wall thickness of the seminiferous tubules for all periods of treatment compared with control groups. As well as a reduction in wall thickness and height of the epithelium in epididymis (head and tail) for all periods of treatment compared with control animals and this decline was significantly at (P<0.05). The current study also recorded a textile damage in the testes and epididymis (head and tail), which summarized the crash of some seminiferous tubules, degeneration of lining cells in tubule, the appearance of vacuole between cells and lifting epithelium from the basement membrane. On this basis, this study has shown for the first time that particles of copper nanoparticles have a negative impact on the effectiveness and activity of the male reproductive system in albino mice.

دراسة تشريحية مقارنة للاجزاء الخضرية وبعض الاجزاء التكاثرية لمراتب من العائلة القرعية Cucurbitaceae في العراق == Comparative Anatomical Study of Vegetative And Some Reproductive Parts For Taxa of The Family Cucurbitaceae In Iraq

اسم المؤلف: باسمة محمد رضا كاظم العبيدي
اسم المشرف: عذية ناهي سلمان المشهداني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - التشريح
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية دراسة تشريحية مقارنة لتسعة مراتب من العائلة القرعية Cucurbitaceae المستزرعة والبرية والتي زرعت في الحديقة النباتية لكلية التربية للعلوم الصرفة ابن الهيثم والمراتب هي : 1 - Citrullus colocynthis Schrad2 - Citrullus vulgaris Schrad3 | The present study included comparative anatomical study for nine taxa of Cucurbitaceae family for cultivated and wild types, which were planted in the garden of College of Education for Pure Sciences/ Ibn Al - Haitham. These taxa included : Citrullus colocynthis Schrad, Citrullus vulgaris Schrad, Cucumis melo Linnaeus, Cucumis melo var.flexuosus Naudin, Cucumis sativus Linnaeus, Cucurbita maxima Duchesne, Cucurbita pepo Linnaeus, Lagenaria siceraria (Mol.) Standl, Luffa cylindrica (Linnaeus) M. Roem. This study focused on some the anatomical characterization as compared study for the first time to these taxa in Iraq. This study included the features of ordinary epidermal cells, leaves petioles, stems, pedicles and sepals of the studied taxa. The results found the appearance of stomata in the stems and petioles in straight line as adjacent planar shape to the epidermal cells and not scattered as found in the leaves, this result was seen for the first time in the current taxa. Furthermore, the stomata have been shown in pedicel and sepals in same type as the stomatal complexes in leaves. The importance of phenotypic variation for the length and types of eglandular trichomes and the presence or not of glandular trichomes were found to carry out the role of indumentum in the different isolated taxa. Additionally, this study included the examination of the cross sections of the roots and also exposed the constituent tissues of it, the variation in the thickness of epidermis, cortex, vascular cambium as well as the arrangement of phloem cells have an important role in the studied taxa. The cross sections of stems, leaves petioles and pedicles showed shape and studied a significant role in the studied taxa and split them into groups.Moreover, this study concluded that the following of above sections from the outside into the inside has an important role to find out their constituent tissue. Thus the results of the study clarified the importance of epidermis tissue through the explanation of the outer and inner tangential walls of their cells and the variation in their dimensions, thickness and kind of stomatal complexes; therefore it has been given the importance of those features in the studied taxa in this study. Cortex tissue showed an extension of angular collenchyma and chloronechyma from stem to pedicel and have shown variation in the thickness in these two layers which was useful in the classification of studied taxa. Vascular tissues differed in the shape of vascular bundle, the number of rows and xylem units and the arrangement of the internal phloem, and all of those characteristics have helped in the studied taxa. In addition, the vascular tissues helped to find their arranged in the stems and found as vascular cylinder shape, while in the petioles and pedicel shown as vascular ribbons were which took the shape of sections petioles and pedicel. The medullar cells and medullary rays have been studied and also their similarity in the morphological characterization and function. Moreover, the anatomical features of the vertical sections of the laminae of leaves in mesophyll tissue have been examined, in the thickness of palaside and spongy layers in midrib, the variation in number and arrangement of vascular bundles have an important role in the studied taxa. In the current study, the ordinary and petal leaves venation and the type of venation have been studied in the studied taxa. Moreover the vertical sections for sepals were examined and also their similarity with laminae of leaves which was useful for studying the constituent tissues of some parts such as pedicel and sepals and anatomical features; and all of the studied characterization helped to study the vegetative parts through following them in studied taxa.

التحري عن الاميبا الحالة للنسج/ الاميبا المتغايرة Entamoeba histolytica/Entamoeba dispar في عينات البراز للمرضى با ستعمال تقنية PCR == Detection of Entamoeba Histolytic /Entamoeba Dispar In Stool Specimens By Using PCR Technique

اسم المؤلف: بثينة عبد الحمزة حسون الزبيدي
اسم المشرف: اخلاص مشرف عيدان
الموضوع العام: علوم الحياة
السنة: 2013
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تسلط الدراسة الحالية الضوء على تشخيص وتحديد نسبة الاصابة بطفيلي الاميبا المرضية المتمثلة بالاميبا الحالة للنسج Entamoeba histolytica وتمييزها عن الاميبا غير المرضية المتمثلة بالاميبا المتغايرة Entamoeba dispar اللتين تتمايزان بشكلهما المتماثل في كلا الطور | This study sheds light on the determination and diagnose of the percentage of infection of like Entamoeba histolytica and differentiate it from the non pathogenic Entamoeba dispar that were morphologically identical in both cysts and trophozoite phase in two different groups : the first group includes 50 stool samples of patients suffering from diarrhea and abdominal pain (symptomatic group) while the second group includes 95 stool samples of patients not suffering from diarrhea and abdominal pain (Asymptomatic group).In addition to that, 20 stool samples were collected from healthy individuals as a control these samples were collected patients of AL - Zafaranyia General Hospital and AL - Alwyia Childhood Teaching Hospital in Baghdad from July, 2011 to May, 2012.These samples belong to different ages (1 year - < 50 years) of different sexes (males and females). Entamoeba samples were diagnosed by using two methods : the first one is the wet mount as a General Stool Examination(GSE) after macroscopic examination for sample and classifying them according to their consistency (solid, semisolid and liquid) and color (brown and yellow) and presence of blood and mucous in addition to diagnosing them microscopically to detect the presence of pus cells and both phases trophozoite and cyst for Entamoeba histolytica.While the second method that was depended in diagnosis is duplex polymerase chain reaction (dPCR) by using two pairs of genes for detection of their amplification in DNA sample isolated from stool. The first pair is cystein proteinase which represents one of virulent factors in Entamoeba histolytica parasite as a tissue invader. The second pair encodes the surface antigen present on the surfaces of both parasites E. histolytica and E. dispar. It is represented by Actin gene (Act). The results of the wet mount showed that in percentage of infection of both species (E.histolytica/E.dispar) the (54%) of patient symptom and (34.37%) patient a symptom.While results of the specificity and sensitivity showed that in positive sample wet mount negative PCR while negative sample wet mount showed that positive PCR and higher percentage of infection the Entamoeba dispar. While the results of the dPCR showed that in spite of the presence of both species Entamoeba pathogenic (Entamoeba histolytica) and non pathogenic Entamoeba (Entamoeba dispar) , the percentage of infection of Entamoeba histolytica was lower than that of Entamoeba dispar. This was clear by the detection of amplification the Reaction producets (dPCR) by using both genes : Actin &Ehcp5 where only 8 samples were detected and they were found to be positive amplification Ehcp5 and 31 samples were positive amplification in both groups of patients (symptomatic and a symptomatic). The percentage of infection in females was higher than that in males for all ages when using both tests (wet mount & PCR). More over the patients with less than (10) years old had higher percentage of infection in both sexes.In conclusion, it should not be depended on direct wet mount technique for the identification of infected parasite Entamoeba histolytica and differentiating it from Entamoeba dispar because of their high homology. That the molecular examination is the only method that is able to determine the kind of infection with parasite Entamoeba

انتاج انزيم السيليلز من عزلة محلية لبكتريا B167 Streptomyces sp. واستخدامه في انتاج الوقود الحيوي == Cellulase Production From Local Isolate of Streptomyces Sp.B167 And Its Application In Biofuel Production

اسم المؤلف: بنان محمود سليمان
اسم المشرف: ناظم حسن حيدر
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية الى انتاج انزيم السليليز من بكتريا الستربتومايسس ودراسة تاثير بعض الظروف الزرعية على انتاج الانزيم; انتاج الوقود الحيوي من المخلفات السليلوزية من خلال التحلل المائي للمخلفات بالاحماض والانزيمات المايكروبية. تم غربلة 74 عزلة لبكتريا St | The current study was aimed to produce biofuel from cellulosic waste material degraded by local isolate Streptomyces. Seventy four isolates of Streptomyces were screened for cellulase production in solid and liquid media. The results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose. Therefore, it was selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the isolate Streptomyces sp. B 167 reached to 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) by supplementation of cellulose liquid medium with 1 % of yeast extract as nitrogen source. Additives of carbon sources like (manitol, glucose, maltose, sucrose and starch) to the process of saccharification did not improve the cellulase productivity. The bioconversion of cellulosic waste to reducing sugar was maximum with Banana peels (77.78 %) followed by the rice husk (75.56 %), orange peels (71.11 %), corn steep peels (60.0 %) and lowest bioconversions (53.33 %) were recorded with sawdust. The degradation of cellulosic waste increased with increasing substrate concentration. Maximum cellulase productivity (3.18 U/ml) and bioconversion (86.1 %) was obtained at 3 % (w/v) of cellulosic waste (Banana peels). Saccharification of cellulosic waste with different treatment methods was studied. The pretreatment of cellulosic waste with 1 % HCl and H2SO4 produces 21 and 15.8 g of reducing sugar / 100 g of cellulosic waste. In comparison, hydrolysis with Streptomyces sp. B 167 enzymes resulted a significantly higher amount of reducing sugar yield (25 g / 100 g cellulosic waste). Further fermentation of cellulosic hydrolysates was performed using Saccharomyces cerevisiae using stationary fermentation condition. Maximum yield of ethanol were (0.30, 0.19 and 0.10 g ethanol / g glucose) observed with Streptomyces sp. B 167 enzymes, HCl and H2SO4 hydrolysates respectively after 48 h of fermentation

التحري عن عدد من المعادن الثقيلة والتلوث البكتيري في بعض الخضروات المعلبة والطازجة المستوردة في مدينة بغداد == Determination of Several Heavy Metals And Bacterial Contamination In Some Imported Canned And Fresh Vegetables In Baghdad

اسم المؤلف: حسين خالد نعمان
اسم المشرف: ايثار كامل الميالي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم اخذ ستة وثلاثين عينة من الخضروات المعلبة وخمس عينات من الخضروات الطازجة, وتجميعها من مناطق واسواق مختلفة من مدينة بغداد بشكل عشوائي خلال الفترة من شهر تشرين الاول 2013 الى شهر نيسان, 2014 حيث تضمنت الدراسة التحري عن تراكير بعض العناصر الثقيلة وهي (الرص | In this study, thirty six samples of canned vegetables and five samples of fresh vegetables were collected randomly from different markets of Baghdad city from October 2013 till April 2014. The study included determining of the concentration of some heavy metals (lead, nickel, zinc and iron) by atomic absorption spectrophotometery and the bacterial contamination in samples, also comparing the canned vegetables with fresh vegetables. It was found that the higher concentrations of heavy metals in canned vegetables as the following : lead 1.179 µg/g in olive, nickel 0.908 µg/g in olive, zinc 10.143 µg/g green pea and iron 90.601µg/g in white asparagus. The lowest concentrations were as the following : lead 0.002 µg/g in green asparagus, nickel 0.019 µg/g in mushroom, zinc 0.528 µg/g in white asparagus and iron 4.061 µg/g in green pea. In fresh vegetables, it was found that the highest concentrations of heavy metals were as follows : lead 0.919 µg/g in green pea, nickel 1.108 µg/g in green pea, zinc 4.304 µg/g in mushroom and iron 43.618 µg/g in tomato. The lowest concentrations were as the following : lead recorded 0.011 µg/g in tomato, nickel recorded 0.022 µg/g in tomato, zinc 0.876 µg/g in green pea and iron recorded 11.081 µg/g in green pea. The identification of the bacteria done by some identification tests for gram negative bacteria, the testes include growth on MacConkey agar, growth on EMB agar, IMViC testes and motility test. The identification tests for Gram positive bacteria included growth on manitol salt agar, growth on staph 110 agar and motility test. The bacterial tests ensured that there is no bacterial growth was detected in the canned vegetables, except some samples (white asparagus, green asparagus and mushroom) of December 2013, while it was detected in fresh vegetables. The bacterial species that isolated in these samples were E.coli, Klebsiela pneumoniae, Bacillus cereus and Staphylococcus aureus.

دراسة شكليائية ونسجية للقناة الهضمية في سمكة الخشني Liza abu Heckel.1843 == Morphological And Histological Study of The Digestive Tract In Liza Abu (Heckel, 1843)

اسم المؤلف: حنان رعد ضياء حسين
اسم المشرف: وجدان بشير عبد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The morphological and histological structure of digestive tract in one species of Iraqi fish that resides in Iraqi inland fresh water (Liza abu) was studied. In this study used (43) fish were in different lengths, the gut of these fish have removed to study the relationship between the length of gastrointestinal tract and the standard body length as well as for morphological and histological study. The value of relative digestive tract length (RGL) was 2.9. Intestine was the longest part of the digestive tract, the average length of intestine was (32 cm). The shape of the folds of the mucous membrane lining the walls of digestive tract showed some differences in different parts of digestive tract, in esophagus the folds were longitudinal running along the wall and it was straight, unbranched and the mean of their number was (9 - 8) folds. In the stomach the folds were longitudinal and (7 - 6) in their number, they were branched into fingerlings structures. In pyloric caeca, anterior and posterior part of intestine and rectum the folds were fingerlings in shape, unbranched and their number were (20 - 19), (27 - 26), (25 - 23), (19 - 18) respectively. Histological study was shown that the wall of the digestive tract is consist of four major coats, from the inside to the outside they were as follows : Tunica Mucosa which characterized by its clear three parts, epithelial tissue, lamina propria and muscularis mucosa. Epithelial tissue in esophagus was thick stratified squamous epithelium, in stomach was thin simple columnar epithelium and gradually increase in length in each of pyloric caeca, intestine and rectum and it was simple columnar epithelial tissue. Mucous which is secreted in esophagus is made of a large proportion of neutral and acidic mucopolysaccharide, while the mucous secreted in the cardiac stomach consist of a large proportion of neutral mucopolysaccharide and less proportion of acidic mucopolysaccharide, either, in the pyloric stomach is consist of very small proportion of both neutral and acidic mucopolysaccharide, in pyloric caeca mucous consist of a small proportion of neutral mucopolysaccharide and less proportion of acidic mucopolysaccharide, and the mucous secreted in an anterior, posterior parts of intestine and rectum consist of small proportion of acidic mucopolysaccharide and large proportion of neutral mucopolysaccharide. Lamina propria consists of connective tissue containing capillaries and lymphoid cells and it represent the basis of the folds, in the cardiac stomach it contains simple tubular glands. Muscularis mucosa characterized by the presence of muscle fibers that are not continuous, the higher thickness of this layer was in pyloric stomach. Tunica Submucosa consists of connective tissue that contains capillaries, the higher thickness of this coat was in the stomach and less thickness of this coat was in the rectum. Tunica Muscularis consists of two layers of smooth muscle fibers, the internal layer has a circular arrangement, the external layer has a longitudinal arrangement in stomach, pyloric caeca, intestine and rectum. either in esophagus tunica muscularis was arranged in inverse manner. The circular and longitudinal muscle fibers in esophagus were skeletal muscle fibers, and they were smooth and non - striated in stomach, pyloric caeca, intestine and rectum. the higher thickness of this coat was in the stomach and less thickness was in the rectum Tunica Serosa is present in all regions of digestive tract.

دراسة بعض متغيرات الخطوط الجلدية للبنان ومجاميع الدم لذكور اطفال التوحد

اسم المؤلف: اسراء جعفر صادق السعدي
اسم المشرف: نصر فرحان عبد الله
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الوراثة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:

التاثير التثبيطي لبعض المستخلصات على فعالية انزيم Angiotensin converting enzyme وبعض المؤشرات الحيوية المساهمة في ارتفاع ضغط الدم == Inhibitory Effect of Some Plant Extracts On Angiotensin Converting Enzyme Activity And Some Biochemical Marker That Associated With Hypertension

اسم المؤلف: رؤى اياد يوسف
اسم المشرف: غازي منعم عزيز | حسن فياض
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study was sought to investigate the inhibitory effect of four crude aqueous plant extracts : Zingiber officinale Roscoe (ginger), Camellia sinensis (Green tea), Olea europaea (Olive) and Hibiscus sabdariffa (Rosella) on key enzyme linked to hypertension, Angiontensin - I Converting enzyme (ACE), and on the oxidant/antioxidants status, lipid profile in vitro and in vivo studies. Study of some biochemical biomarker demonstrated that antioxidant enzyme, oxidant enzyme, liped profile and ACE level for 75 hypertension patients. Antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) were shown to have cross relationship with ACE level in hypertension groups, while lipid profile have positive relationship with ACE level in hypertension groups. ACE activity for the four groups G1, G2, G3, G4 were 19.61±3.97, 21.3 ± 1.95, 28.06 ± 5.34 and 32.74±8.19 ng/ml respectively. From these results we concluded that ACE was associated with hypertension and its modulated by drug or herbal extracts. Angiotensin - I Converting enzyme was extracted from sheep lung with specific activity 0.08 U/mg, then the crude ACE extract was concentrated with sucrose by dialysis with specific activity 0.1 U/mg, purification fold was 1.25.The enzyme was purified partly by ion - exchange chromatography using DEAE - cellulose with specific activity 0.5U/mg, yield 30% and purification fold 6.25. ACE activity was determined using N - [3 - (2 - furyl) acryloyl]L - phenylalanyl glycyl glycine (FA - PGG) as a substrate. Results for in vitro ACE inhibitory activity using plant extracts (ginger, green tea, roselle and olive) showed that the all four crude aqueous plant extracts had inhibitory activity in different values when used in the same concentrations about (1 mg/ml), and ginger extract possessed higher inhibitory activity than other three extracts. The ACE inhibitory potency of the ginger extract was found to be significant (P<0.001) when compared with the standard anti - ACE inhibitor drug (Captopril) at the same concentration. The inhibitory activity of ginger extract with different concentration (25, 50, 100 mg/kg) in L - N - ? - nitro - L - arginine methyl ester (L - NAME) induced hypertensive mice was evaluated. Acute oral administration with L - NAME 50mg/kg.b.w causes a rise in blood pressure in normal mice. Administration of aqueous ginger extract (25, 50 and 100 mg/kg) for 4 weeks in L - NAME treated mice significantly (P<0.05) reduced the mean arterial blood pressure compared with L - NAME animals without treatment, with decreasing the serum levels of ACE; while the activity of superoxide dismutase (SOD) and glutathione peroxidase(GPx) showed a significant elevation in ginger treated L - NAME induced hypertensive mice. The results suggest that ginger extract could prevent the development of high blood pressure induced by L - NAME probably can be attributed to prevent or reduce the oxidation process and the inhibition of physiological processes of a substance L - NAME and so as it contains ginger compounds of polyphenols, which inhibits the activity of the ACE and prevent oxidation of fats and repair System Antioxident. Our study concluded that ginger might act as a natural alternative to better and safer in the prevention of negative impacts and risk factors such as high blood pressure and lipids.

تاثير انزيم SNase المنقى من العنقودي في الغشاء الحياتي للايشيركية القولونية والكليبسيلا الرئوية == Impact of Snase Purified From Staphylococcus Aureus On Biofilm of Klebsiella Pneumoniae And Escherichia Coli

اسم المؤلف: هند تحسين ابراهيم
اسم المشرف: حارث جبار فهد المذخوري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: SDS - PAGE showed that a single sharp band with an approximately 16.8 KDa molecular weight has been seen. A matter indicates that the enzyme is consistently pure. PCR technique was applied to approve the existence of nuc gene in S.aureus Nevertheless, only those which depicted positive results on DNase agar harboured nuc gene, as it is specified by single band appearance of nuc at assumed molecular weight (ca. 255 pb) of marker that being used. The current study findings highlighted the participation of SNase purified from S. aureus in significant (P< 0.05) preventing biofilm formation by E. coli and K. pneumoniae compared with untreated controls. Evidently, the inhibitory effect of SNase on biofilm formation is undoubtedly perceived, indicating the degradation of the structural major component of biofilm formation (i.e. extracellular DNA). Results revealed that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; E. coli and K. pneumoniae attached to the uroepithelial cells. 1 Chapter one : Introduction and literature Review 1. Introduction and Literature Review 1.1. Introduction Staphylococcus aureus is a persistent human pathogen that is responsible for a range of diseases that vary widely in clinical presentation and severity. The pathogenesis of S. aureus infection is a complex process involving a diverse array of secreted and surface - associated virulence determinants that are coordinately expressed at different stages of infection (Loughman et al., 2009). Escherichia coli is a genetically diverse species that causes a variety of infections which fulfill many or all of the proposed criteria for biofilmassociated infections (Kaper et al., 2004). Klebsiella pneumoniae is an opportunistic pathogen responsible for a wide range of nosocomial infections. One important factor associated with virulence in K. pneumoniae is its capacity to adhere to surfaces and form biofilms (Cruz et al., 2012). Bacteria often exist as sessile communities called biofilms which are exquisite structures caused by a genetically programmed developmental process. It is estimated that biofilms are involved in 65% of human bacterial infections, since cells in biofilms are 1000 times more resistant than cells in the planktonic state, making medical treatments fail (Shal? et al., 2011). Extracellular DNA (eDNA) plays a significant role in biofilm formation, as revealed by studies in several bacteria including E. coli (Nakao, 2012) and K. pneumoniae (Whitchurch et al., 2002); however, there is no definite proof on a cause - and - effect relationship between DNA release and biofilm formation (Beenken et al., 2012) or becomes a key component of the macromolecular scaffold in many different biofilms (Jakubovics et al., 2013). In rare cases eDNA has been shown to inhibit bacterial settlement. Therefore, it is possible that nuclease mediated eDNA degradation would therefore promote adhesion. Consequently, it is not clear whether microbial nucleases contribute to the gross biofilm structure in clinically relevant situations (Sheilds et al., 2013). 2 Chapter one : Introduction and literature Review All previous studies used both commercial bovine and recombinant human DNaseI in the disruption of medically important biofilms; whilst, extracellular nuclease of S. aureus (SNase) was used against biofilm of S. aureus themselves (Benenken et al., 2012b ; Kiedrowski et al., 2014). Thus, to date, the role of S. aureus exonucleases in biofilm of other bacteria remains unclear. However, in the present study SNase is used against biofilm of other bacterial species (viz. K. pneumoniae and E. coli). To address this, the following steps were undertaken : 1 - Investigating the negative impact of SNase extracted from S. aureus on K. pneumoniae and E.coli biofilm formation. 2 - Studying the preventive activity of SNase on the adherence of K. pneumoniae and E. coli on uroepithelial cell.

دراسة بيئية لبعض انواع صنف بطنية القدم الارضية في ثلاث محافظات وسط العراق == An Ecological Study For Some Terrestrial Gastropoda Species In Three Provinces Middle Iraq

اسم المؤلف: ايمان حسين عبد
اسم المشرف: عماد الدين عبد الهادي المختار
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية للتعرف على انواع صنف بطنية القدم الارضية المتواجدة في بعض محافظات وسط العراق (بغداد, بابل, كربلاء). اذ تم تسجيل الملاحظات الحياتية والتعرف على بيئاتها المفضلة وقياس العوامل البيئية ذات الاهمية. حيث تم تشخيص ثلاثة انواع وهي : Cornu as | The present study was conducted to identify the terresterial gastropoda species located in some provinces of middle Iraq (Baghdad, Babylon and Karbala). They were record Biological observations on their environment and learn about their prefer environments and to measure the important ecological factors. Three species were diagnosed : Cornu aspersum specie belong to Helicidae family, Monacha cantiana and Candidula gigaxii species belong to Hygromiidae family. Monthly sampling was conducted from (October 2013 - July 2014) of the three provincs on two sites per each provinc, Where the samples were collected randomly by five repeaters of one square meter once a month. The study also included soil ecological properties measuring : soil temperature, soil pH, Electrical conductivity, percentage moisture, organic matter content and soil texture.The results showed soil temperature values ranged between (8 - 30) C?, soil pH (7.33 - 7.96), Electrical conductivity (0.7 - 4.1) ds/m, percentage moisture (9 - 33) % , organic matter content (0.81 - 2.88) %. The results showed percentages rate for clay, silt and sand for Jadiriyah (38.39, 49.18, 12.43)%, Zafaraniya (36.66, 51.53, 11.81)%, Alexandria (37.93, 52.54, 9.53)%, Hindyia dam (40.54, 53.05, 6.41)%, Hosseinie (36.28, 45.81, 17.91)% and Twerij (37.79, 50.20, 12.01)%. Generally soil texture clay silt sandy soil for all study sites. The relative abundance of the studied species was recorded, The lowest value for Candidula gigaxii specie was 7.3 % in March in Hindyia dam, while the highest value was recorded for Cornu aspersum specie 75.0 % in December 2013 in Jadiriyah. Rates of population density (individuals / m 2) were calculated for the studied specimens and the results showed the presence of significant differences when comparing the study sites during research period. The population density rates for species the results showed the lowest value for Candidula gigaxii specie 0.4 (individual/m²) in October 2013 in Jadiriyah, and the highest value for Cornu aspersum specie 19.4 (individual/m²) in Janeuary 2014 in Jadiriyah. The results showed that Candidula gigaxii specie is found in sites (Jadiriyah, Zafaraniya, Hindyia dam(along research period, While the results did not record the presence of it in sites (Alexandria, Hosseinie, Twerij).The study showed presence of positive and negative significant relationships between species and soil ecological properties, as it were for Cornu aspersum specie negative significant relationship with air and soil temperature (0.827, 0.763) respectively, positive significant relationship with moisture and organic matter content (0.703, 0.880) respectively, while the results did not record significant differences with pH and Electrical conductivity (0.096, 0.168) respectively. Monacha cantiana specie has recorded negative significant relationship with air and soil temperature (0.431, 0.553) respectively, positive significant relationship with percentage moisture and organic matter content (0.368, 0.550) respectively, while did not record significant differences for pH and Electrical conductivity (0.214, 0.096) respectively. The results showed for Candidula gigaxii specie negative significant relationship of air and soil temperature (0.504, 0.322) respectively, positive significant relationship with moisture and organic matter content (0.380, 0.569) respectively, While did not record significant differences with pH and Electrical conductivity (0.088, 0.170) respectively.

تحديد تعبير الجين المتحمل للملوحة TaGSK1 في عدد من اصناف الحنـطـة == Determination of Gene Expression of Salt Tolerant Gene Tagsk1 In Wheat Cultivars

اسم المؤلف: ايمان نعمان اسماعيل
اسم المشرف: نورية عبد الحسين علي | مجيد ارشيد سباح
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نفذت الدراسة الحالية في مركز بحوث التقنيات الاحيائية/ جامعة النهرين للمدة 2011 - 2012 لغرض الكشف عن جين الملوحة TaGSK1 ودراسة التعبير الجيني له في صنفين من الحنطة فرات ودجلة والتركيب الوراثي 2H المنتخبة لصفة تحمل الملوحة مقارنة مع الصنف تموز2 الحساس للملو | This study was conducted in biotechnology research center, Al - Nahrain university in 2011 - 2012 to detect the gene responsible for salt tolerant (TaGSKI) and study its expression in two wheat (Tigris and Euphrates) cultivars and the genotype (2H) were selected for salt tolerance through improvement and breeding programs as compared with to the sensitive local wheat cultivar (Tamooze 2). Seeds of the these cultivars were sown in flasks that contained Ms media in three salt levels (0, 15, 25) ds/m with three replication for each. Five seeds from each cultivars were sown in each replicate. After 10 - 15 days from the sowing, the percentage of germination was estimated, and samples of leaves were collected for RNA extraction and then changed to the cDNA. B - actin gene that consider endogenous control and salt tolerant gene TaGSK1 were amplificated by using PCR technique to discover the gene and by QPCR to estimate gene expression by determine the CT (Cycle Threshold) value for B - actin and TaGSK1 genes in wheat plants of the studying cultivars. Number of DNA dilutions of 189bp molecular weight that extracted from agarose gel were used to determing CT value for each dilution. Standard curves were drawn to find out value of PCR Efficiency which was used for gene expression for the salt tolerant gene TaGSK1.The results of germination percentage (%) showed that there were high differences between the two salt tolerant cultivars (Tigris and Euphrates) and 2H genotype and local cultivar (Tamooze 2). Euphrates cultivar gave 100% germination percentage, and the Tigris cultivar and 2H genotype gave 89, 86% germination percentage respectively at 25ds/m. whilst the local cultivar gave zero germination percentage at the same level of salinity. At 15ds/m level, also the Euphrates cultivar gave 100% germination percentage while the Tigris cultivar and 2H genotype gave 96, 94% germination percentage respectively as compared to Tamooze cultivar that gave 13% germination percentage. All the cultivars have 100% germination percentage at 0.0ds/m level. The conclusion of this result is the two cultivars (Tigris and Euphrates) and 2H genotype more salt tolerance than local cultivar at this growth stage which more salt sensitive than others growth stages. The results of PCR reaction were also indicated that Tigris and Euphrates cultivars and 2H genotype have salt tolerant gene TaGSK1, while this gene did not exist in the local cultivar. There were two bands of TaGSK1 gene that have 189bp and 404bp molecular weight in the Tigris and Euphrates cultivar and the genotype 2H, while the local cultivars have only one band that have 404bp molecular weight. These results were indicated that 189bp molecular weight of this gene is responsible for salt tolerance character in these cultivars.The results of QPCR reaction also were revealed that there is difference between the cultivars in their gene expression. Tigris and Euphrates cultivars and 2H genotype gave the highest gene expression at 15ds/m and increased at 25ds/m as compared to 0.0ds/m. At the second level 15ds/m the gene expression of the two cultivars and genotype was 0.8682, 0.8190 and 0.8688 respectively, and at the third level 25ds/m was 1.656, 1.3176 and 1.2665 respectively, while at the first level 0.0ds/m was less than the other 15, 25 ds/m as compared to local cultivar that the gene salt tolerant (TaGSK1) have no gene expression at the same salt levels. This result indicated that the local cultivar does not have salt tolerant gene TaGSK1. From these results we can revealed that TaGSK1 gene was found in the Tigris and Euphrates cultivars and 2H genotype, and this gene can be considered from high salt tolerant gene, because it gave the highest gene expression at the highest salt level 25ds/m. Therefore this gene help the plant to tolerate salt stress and grow very well. The results also showed that the two salt tolerant cultivars (Tigris and Euphrates) and the salt tolerant genotype (2H) proximately have the same salt tolerance degree, so they have proximately the same gene expression as compared to salt sensitive local cultivar which have no salt tolerant gene TaGSK1. This gene is good indicator for salt tolerance at high salinity levels 15, 25 ds/m in these cultivars and genotype.

التاثيرات المرضية النسجية لعقار ليفيتيراسيتام في التكوين الجنيني لكلى اجنة ومواليد وحوامل الجرذ الابيضAlbino rats (Rattus rattus) == Histopathological Effect of Levetiracetam Drug On The Kidney Development In Rats Embryo, Newborns And Pregnant Albino Rat (Rattus Rattus)

اسم المؤلف: لمياء هادي محمد الابراهيمي
اسم المشرف: نهلة عبد الرضا البكري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الاجنة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الصرع هو حالة مزمنة تتميز بنوبات متكررة دون سابق انذار، سببها خلل في نقل الاشارات الكهربائية داخل الدماغ، وعقار Levetiracetam (LEV) من العقاقير المضادة للصرع، ذو خصائص دوائية ملائمة وتظهر فعاليته في تحسين السيطرة على النوبات، ويكون امتصاصه في البلازما سري | Epilepsy is a condition characterized by recurrent abrupt seizures episodes caused by the defect in the transmission of electrical signals to the brain. LEV is one of antiepileptic drugs which has appropriate pharmacological properties and show its effectiveness in improving the control of the epileptic seizers, its rapid absorbance, and its excretion through the kidneys by glomerular filtration and it is re - absorption into the tubular portion.Kidney arise from intermediate mesoderm and it consists of three execratory organs in all vertebrates : pronephros which is transient and present in all vertebrates, mesonephros which also present in all vertebrates embryos and their activity depends upon the type of embryo, and metanephros which is the functional kidney during life after the embryogenesis in reptiles, birds and mammals. The first signals for kidney initiation in albino rat embryo take place in the 10th days of pregnancy. The current study is designed to demonstrate the effect of LEV (350 mg/kg/day) on the kidney development of Albino rat fetus from the age of 8 - 18 days of pregnancy and its effect on the kidneys of the 3 and 7 days aged newborns. In addition to its effect on pregnant rat kidneys treated with the drug for 17 days. Hundred pregnant Albino rat (Rattus rattus) are enrolled in this study, classified blindly to 50 control and 50 pregnant rats are orally treated with LEV (350 mg/kg/day). The histological results of the current study revealed that there is no effect on embryonic kidney in fetuses aged 9 days of pregnancy, whereas, the pronephros of the fetuses aged 10 days shows death of the cells in comparison to control group. Moreover, the kidney of the 11th aged fetuses showed degeneration of the mesonephric duct represented by decrease cellular mass, death and hypertrophy of some cells comparing to the cells of control group. Concerning the kidney of the 12th aged fetuses and subsequent days, there is degeneration of the epithelial cells of the metanephros duct, their detachment from the basement membrane and their congestion in comparison to the cells of the control group. The kidney of the 13th aged fetuses showed swelling, damage for some of the epithelial cells of the glomerulus and collecting tubules, accumulation and shrinkage of glomerular cells, damage, death, and detachment of some collecting tubules cells demonstrated in the 14th aged fetuses. There enlargement of capsular space in addition to the most previous defects was seen in the 15th aged fetuses in comparison to the control group. Most of the previous defects are seen in the 16th, 17th and 18th age fetuses. Scanning electron microscope revealed nuclear enlargement of the podocytes found in the visceral layer of the renal corpuscle of the 16th aged fetuses (mean diameter 64.6 µm compare to 39.2 µm of the control group). Regarding the kidney of 3rd and 7th aged newborns, the histological defects seen in the latest aged fetuses was also seen and there was statistically significant differences (p?0.05) between the diameters mean of the glomerulus of the 3rd and 7th aged newborn. Whereas, there was no significant difference in the diameter of the kidney tubules of 3rd but there was significant difference of the 7th aged newborn. Regarding the kidney of the 17th day treated pregnant rat, there was enlargement of the capsular space caused by accumulation, and degeneration of glomerular cells characterized by loss of the nucleus, shrinkage of the glomeruli, and atrophy of most of them. Loss of the nucleus and detachment of the cells, and swelling of most of the collecting tubules. There was a statistically significant difference between the diameter of the kidney tubules of the pregnant rat and the control group. In conclusion, LEV had histological effect on the kidney of the 10 - 18 aged fetuses and on 3, 7 days aged newborns in addition to its effect on the kidney of the pregnant female treated with the drug for 17 days.
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