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تحضير وتشخيص بعض المشتقات الجديدة للبنزايميدازول المعوضة Synthesis And Characterization of Some New Substituted Benzimidazole Derivatives

المستخلص: The project conducted in this research work was carried out from December 2013 to June 2014 which leads to this thesis, which are in five chapters. All these chapters cover up the survey, the practical part of preparing, manufacturing and using Atomic force Microscopy that have been adopted during this research work, and the results were subjected into various internationally used mathematical and data treatments.Chapter OneDiscusses the acids, ion exchange, precipitation reaction, crystal growth theories, light scattering, turbidity and describe FIA in general, it is classification and fundamentally involved ideas without forgetting the use and applications of FIA. Chapter one also discusses derivative spectroscopy, definition, classification, the applications. This chapter ends with the aim of the project.Chapter Two : In this chapter a complete description of the chemicals and ion exchange column, their preparations and their use throughout this project. This chapter describes the use of flow system with all it is components (i.e. peristaltic pump, connection tubes, junction(Y - junction), six ports injection valve, ion exchange column and measuring readout system). Chapter Three : Consists of four parts The first part describes the design of Ayah 5SX1 - T - 1D Solar cell CFIA and deals with calculation of dispersion to evaluate the change in concentration ateach location of the designed manifold system via the chasse of an air bubble. The second part deals with the analysis carried out in this research work. Determination of oxonium ion (HClO4, HNO3, H2SO4 and HCl) via turbidity of solution of zinc (II) ion in cation exchange column - K3[Fe(CN)6] system. A comprehensive detailed study was carried out using Ayah 5SX1 - T - 1D Solar cell CFIA microphotometer. The linear range (10 - 100 mMol.L - 1), limit of detection (43.20, 57.14, 30.28, 30.97 ng/sample) for above mentioned acids and percentage of relative standard deviation < 2% for five successive measurement of 20 and 80 mMol.L - 1 for used acids. The method was applied successfully for determination of the mentioned oxonium ion in pure and commercial samples. A comparison was made between the newly developed method and the classical method pH meter using of paired t - test. It was noticed that there was no significant difference between two methods.The third part deals with the analysis carried out in this research work. Determination of oxonium ion (HClO4, HNO3, H2SO4, HCl) via turbidity of solution of copper (II) ion in cation exchange column - K3[Fe(CN)6] system. A comprehensive detailed study was carried out using Ayah 5SX1 - T - 1D Solar cell CFIA microphotometer. The linear range (10 - 100) mMol.L - 1, limit of detection (42.94, 39.14, 20.61, 26.11 ng/sample) for above mentioned acids and percentage of relative standard deviation < 2.5% for five successive measurement of 20 and 100 mMol.L - 1 for used. The method was applied successfully for determination of the mentioned oxonium ion in pure and commercial samples. A comparison was made between the newly developed method and the classical method pH meter using paired t - test. It was noticed that there was no significant difference between two methods.The fourth part deals with the approache that was used through linear array Ayah 5SX1 - T - 1D continuous flow injection analyzer to distinguish between the responses that were obtained i.e. discrimination between the kind signals that were completely different.Chapter FourDeals with studying topography and morphography of the Zn3[Fe(CN)6]2 and Cu3[Fe(CN)6]2 complexes using atomic force microscopy. Chapter Five : This chapter concludes few interesting points based on the obtained results throughout this research work, such as conclusions and future work, Published work, and references. -
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الكيمياء التحليلية
السنة: 2014
الموضوع العام: علوم الكيمياء
اسم المشرف: عبد الامير مطلك فنجان
اسم المؤلف: سحر ثامر عداي

تقدير السبروفلوكساسين, النورفلوكساسين والحديد الثلاثي باستعمال الطرق الطيفية مع الاستخلاص بنقطة الغيمة Determination of Ciprofloxacin, Norfloxacin And Iron (III) Using Spectroscopic Methods With Cloud Point Extraction

المستخلص: يتضمن البحث تحضير ستة وثلاثون مشتق جديد من مشتقات]2 - (5 - كلورو - 1H - بنزو]د[ايميدازول - 2 - يل انيلين[، وقد تمت دراستها وتشخيصها بواسطة اجراء تحاليل اطياف الاشعه تحت الحمراء FT - IR)) واطياف الرنين النووي المغناطيسي للبروتونH - NMR)1) لها.تم تقسيم تح | This research includes synthesis of thirty six new derivatives of [2 - (5 - chloro - 1H - benzo[d]imidazol - 2 - yl)aniline] and these derivatives were characterized by their FT - IR and 1H - NMR spectra.The synthesized compounds were divided into three parts : - The first part involved : - 1 - Synthesis of 2 - (5 - chloro - 1H - benzo[d]imidazol - 2 - yl) aniline [1] by the reaction of 4 - chloro - 1,2 - diaminobenzene with anthranilic acid and hydrochloric acid as a reagent.2 - Acetylation of compound [1] by using acetic anhydride in order to obtaine the Acetamide compound [2]. 3 - Synthesis of N - [2 - (5 - chloro - 1H - benzo[d]imidazol - 2 - yl) phenyl) - hydroxyl N' - methyl] acetimidamide [3] by the reaction of compound [2]with hydroxyl amine hydrochloride and sodium carbonate.4 - Synthesis of Benzimidazolyl Chalcone derivatives [4 - 11] by the reaction of the compound [2] with various aromatic aldehydes such as benzaldehyde ,4 - nitro benzaldehyde,4 - chloro benzaldehyde and 4 - methyl benzaldehyde.The second part included : Mannich reaction was carried out based on the compound [1] using formaldehyde and different secondary amines such as piperdine , indole, diphenyl amine , morpholine , indole - 3 - acetic acid , and imidazole to offered [12 - 24]. The third part was synthesized as following : - 1 - Synthesis of Schiff bases [25 - 32] from the reaction of compound [1] with various aldehydes such as benzaldehyde, 4 - bromobenzaldehyde, 4 - hydroxy benzaldehyde, and glutar aldehyde.2 - Reaction of Schiff bases [26 - 29] with mercaptoacetic acid to give new compounds containing thiazolidin ring [33 - 36].
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الدرجة: ماجستير
الموضوع الدقيق: الكيمياء العضوية
السنة: 2014
الموضوع العام: علوم الكيمياء
اسم المشرف: زهير عبد الامير خماس
اسم المؤلف: نورا سعد مبدر

دراسة التاكل والحماية من التاكل لسبائك من حديد الفولاذ في ماء البحر باستخدام دقائق اوكسيد الزركونيوم وكربيد السليكون واوكسيد الالمنيوم النانوية Corrosion And Corrosion Protection Studies of Carbon Steel Alloy In Seawater Using; Zirconia, Silicon Carbide And Alumina Nanoparticles

المستخلص: يعتبر اوكسيد النحاسوز واحدا من المواد ذات الاستخدامات العلمية المتعددة الاغراض وذات الاهمية الصناعية الكبيرة ,ومنها استخدامه كمتحسس للغازات وفي العوامل المساعدة وكذلك في انتاج الواح الطاقة الشمسية ويدخل وبصورة كبيرة كمحطم للصبغات العضوية في مجال الكيمياء | Cuprous oxide (Cu2O) has been extensively studied because of its potential use in several electronic applications, which include solar cells and gas sensors. This Thesis is devoted to the synthesis, characterization of nano - structured cuprous oxide by using a simple electrochemical cell with a potassium chloride solution as an electrolyte, the electro cell contains a two high purity copper electrodes as a sheet form in different areas at varying sizes the area of the cathode was twice of the area of anode , the two electrodes zone were separated using a fine diameter silica diaphragm,Within this broad scope , the Thesis focuses on : • Production of different shape nano - crystals (Cu2O) and investigation of the correlation between experimental parameters and resulting microstructure.• Production of highly purity nano - crystalline Cu2O powder, with the estimation it’s by the available investigation instruments.• Study the different parameters, current density, bath temperature, KCl concentration , types of additives , time of precipitation, and there’s effects on the shape ,quantity ,surface roughness, powder color, on the Cu2O powder. Models have been proposed for the nano - crystal formation validated by several techniques uses such as X - ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), and Fourier Transform Infra - Red spectroscopy (FTIR). The produced nanocrystals show good crystallinity with Cu2O purity and Copper oxide semiconductor nanoparticles were prepared by electrochemistry using galvanostatic and potentiostatic approaches. The optimum deposition conditions ( electrolyte composition , medium pH , type of additives, electrodes area ,current density , temperature of the electrolyte and applied potential of the cell) , to prepare the Cu2O nano particles have been identified; in particular the conditions that allow getting this product by using KCl ,(PVA ,SDS ,and glycerin as emulsifying agent) have been well identified for the first time. The configuration of deposited Cu2O , purity and amounts. These values depend on the electro deposition conditions such as the pH of the solution, the deposition potential and temperature. Where the study results showed that there is direct effect of these factors, it has been observed that the sizes of the precipitate crystalline decreased with increasing current density and especially at high bath temperatures its ranged about 10 - 20 nm , in addition to the effect of the percentage of additives emulsifier agents the best results at poly vinyl alcohol.. The X - ray diffraction pattern and 2? values reflect that the high purity of the deposit where obtained, without any of the other undesirable products like CuO, CuCl2 and CuCl. Also the FTIR analysis was considered. the effect of additives of the type of emulsifier on the crystalline form of the deposit, the PVA produced of the Star (Branches) crystals shape, the addition of the SDS compound leads to the form of the cubic , and the glycerin product the binary pyramid crystals shape.. Finally the conditions effect on the surface roughness of the precipitated powder also discussed. Depending of the electro - deposition conditions, different surface morphologies with various preferential crystal orientations were obtained for the temperatures of the electro - deposition of 80 °C , pH = 8 - 9 , The influence of several deposition parameters of the Cu2O, such as applied potential, pH, the temperature of the bath , the chemical composition, grain size and orientation parameters of the sample was systematically studied using X - Ray Diffraction , Atomic Force Microscopy, Transmission Electron Microscopy ,Fourier Transmission Infra - Red, and scanning electron microscopy.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الكيمياء الفيزيائية
السنة: 2014
الموضوع العام: علوم الكيمياء
اسم المشرف: خلود عبد صالح السعدي
اسم المؤلف: حيدر عبد الكريم يوسف

دراسات ثرموداينميكية وحركية لفعالية مركبات متعدد الفينولات الطبيعية للوقاية من التسمم بالرصاص Thermodynamic And Kinetic Studies On The Effectiveness of Nutritional Origin Poly Phenols In Lead Poisoning Protection

المستخلص: يتضمن البحث تحضير مركبات حلقية غير متجانسة جديدة ( خماسية , سداسية وسباعية )ودراسة الفعالية الحيوية لها.المرحلة الاولى : تتضمن مفاعلة بنزين ثنائي الامين مع ثنائي كبريتيد الكاربون في الايثانول المطلق للحصول على المركب [1] ليتفاعل مع ( حامض الماليك , حام | This research involves synthesis of new heterocyclic compounds(five , six , and seven - membered rings) and study their biological activity First Part : The o - phenylenediamine was condensed with carbon disulfide in abs. ethanol to yield compound [1] which reacted with(maleic acid and oxalic acid ) and presence of phosphorus oxychlorid to yield compounds [2,3].Second Part : The phthalic anhydride was condensed with glycine in aceton to give compound [4] which reacted with o - phenylenediamine in presence of phosphorus oxychlorid to give compounds [5] and with o - phenylenediamine in abs. ethanol to give compound [6] which reacted with maleic acid in presence of phosphorus oxychlorid to give compound [7]. Third Part : Thiosemicarbizide was condensed with carbon disulfide in abs. ethanol and presence of sodium carbonate to result compound [8] which reacted with different sugars (ribose , xylose and arabinose) in abs. ethanol and presence of glacial acetic acid to give new Schiff bases derivatives [9 - 11] respectively. The resulting imine derivatives were reacted with ( salicylic acid , 2 - mercaptobenzoic acid and glycine) to give compounds [12 - 14] respectively.Fourth Part : Study of biological activity of some synthesized compounds
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الكيمياء
السنة: 2014
الموضوع العام: علوم الكيمياء
اسم المشرف: الهام مجيد الرفيعي
اسم المؤلف: نجدت رضا حميد علي الخفاجي

تاثير الصفائح النانوية على الخواص الفيزيائية والكهربائية لبعض المركبات البلورية السائلة Effect of Nanosheets On The Physical And Electrical Properties of Some Liquid Crystalline Compounds

المستخلص: هذه الاطروحة تمثل سلسلة من دراسات الكم من خلال التركيب الالكتروني للانظمة العضوية الفلزية. تتضمن نتائج الفصول خلاصة عمل تعود لكيمياء الروثينيوم (الفصل الثاني) واستخدام النظريات لتساعدنا على التشخيص التركيبي من خلال اجهزة الطيف الحاسوبية (الفصل الثالث والر | This thesis presents a series of quantum chemical studies into the electronic structure of organometallic systems. All of the results chapters are self - contained, summarising work related to Ruthenium chemistry (Chapter 2) and the use of theory to aid structural identification through computation of spectroscopic parameters (Chapters 3 and 4). Chapter 5 relates to distinct work done concerning to the Topological Analysis of the Electron Density in the dihydried Triruthenium Cluster, but the focus on the question of dihydried triruthenium motif represents a close link to the material covered in Chapters 3 and 4. In Chapter 2, Density Functional Theory (DFT) is used to probe the structures of triruthenium clusters Ru3(CO)12. The researcher shed light on the equilibrium geometries and the best function (PBE1PBE) and basis set (SDD Ru/ SVP on (C, O)) can be used. On the basis of that, the global minimum found for Ru3(CO)12 is an unbridged (1D3) structure. In Chapter 3, DFT with PBE1PBE functional and (SDD Ru/ IGLOIII, TZVP P, H/ SVP on (C, O)) basis sets have been used to identify structures of species observed in hydrogenation reactions of alkynes using catalysts based on Ru3 clusters. The identification of the hydride intermediates observed by NMR spectroscopy is ambiguous, and is generally based only on 1H NMR and JP - H coupling constants for hydrides attached to the ruthenium metal centres. Our calculations propose that the experimentally observed species A and B do not contain the Ru3(? - H)(H) motif, as proposed by Duckett group but have Ru3(? - H)2. In Chapter 4, these studies were extended to the analogous dihydride triruthenium monodentate and bidentate phosphines. By computing energies, 1H chemical shifts, we propose that the experimentally observed species A1 and B1 do not contain the Ru(µ - H)(H) motif, as proposed by Duckett and co - workers, but rather the dibridging Ru(µ - H)2 structures common to A2 and B2. In addition, the results confirm that our methodology is able to model the environment of a terminal hydride correctly.Finally, Chapter 5 describes the Quantum Aim (QTAIM) topological analysis of the electron density in the Picolyl N - Heterocyclic Carbene Triruthenium cluster [Ru3(? - H)2(?3 - ?3C2,NHCpyCH2ImMe)(CO)8] (3 - methylimidazol - 2 - ylidene). However, this cluster has been chosen since, firstly, has Ru3(? - H)2 motif similar to triruthenium core proposed in chapters 3 and 4 , secondly, this particular cluster contains one hydride - unbridged and two hydride bridged Ru - Ru edges and a face - capping ligands with different types of Ru - C and Ru - N bonds , this allowing interesting comparisons between the topological properties of related but different atom - atom interactions, within the same molecule.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الكيمياء
السنة: 2014
الموضوع العام: علوم الكيمياء
اسم المشرف: عصام عبد الكريم عبد اللطيف عمار هاني الدجيلي
اسم المؤلف: الاء فاضل سليمان داود

العلاقة بين المشعرات المهبلية والعوامل المرضية التناسلية الاخرى بين النساء في مدينتي كركوك وتكريت Relationship Betwee Trichomonas Vaginalis And Other Genital Infectious Agents Among Women In Kirkuk & Tikrit Cities

المستخلص: تم خلال الفترة من الخامس عشر من شهر تشرين الاول 2006 ولغاية الثلاثين من شهر حزيران 2007 اجراء دراسة عن وبائية طفيلي المشعرات المهبلية وبعض مسببات الالتهابات المهبلية الافرازية المرافقة وغير المرافقة لها بين النساء المراجعات لقسم الاستشارية النسائية والتول | An epidemiological study was carried out from 15th, October 2006 to 30th, June 2007 for prevalence of Trichomonas vaginalis parasite and some causative agents of secretary vaginal inflammations associated or non - associated with the parasite among women attending the department of Obstetrics and Gynecology in two General hospitals in Kirkuk city and Tikrit teaching hospital in Tikrit city. A total of 300 samples (200 in Kirkuk, 100 in Tikrit) were colle - cted from (15 - 47) years old women. Two swabs were taken from the vaginal (high vaginal) and the lining of uterus cervix (endocervical) for each woman by Gynecologic physician. A gross examination of the swabs was done to determine the color of vaginal secretions and measurement of pH. A wet preparation was done from the (high vaginal swab) and examined microscopically for diagnosis of Trichomonas vaginalis and Candida spp. In addition a Gram stain smear was prepared from a portion of the same swab and examined by oil - immersion for diagnosis of Neisseria gonorrhoeae and Trichomonas vaginalis. A chemical test was done for the detection of Gardnerella vaginalis bacteria & Candida spores. The swabs prepared from endocervical were treated by ACON - Chlamydia Kit specific for diagnosis of Chlamydia trachomatis in vaginal swabs samples. The study concluded the following results : 1 - The infection with various sexually transmitted diseases agents were 68% , 78% in Kirkuk & Tikrit cities respectively (P>0.05).2 - The maximum infection rate for T. vaginalis was 19.5% in Kirkuk city & 16% in Tikrit city among co - infection with other agents and single infections.3 - The percentages of single infections with T. vaginalis alone for various age groups were 16.5%, 8% in Kirkuk and Tikrit cities respectively (P>0.05).4 - The rate of infections with various vaginal micro - organisms were in the following sequence : T. vaginalis (19.5%), G. vaginalis (18.5%), C. trachomatis (14%), Candida spp. (11.5%) & N. gonorrhoeae (4.5%) in Kirkuk city, whereasin Tikrit city the sequence of infection rates were as follows : G. vaginalis (43%), T. vaginalis (16%), C. trachomatis (12%), Candida spp. (6%) & N. gonorrhoeae (1%).(P>0.05).5 - The highest rates of infections with T. vaginalis only as a single infecting agents were 21.27%, 10% among a group age range (36 - 45) years in both Kirkuk & Tikrit cities respectively (P<0.05). 6 - The maximum rate of infection with the T. vaginalis were recorded among women with yellowish vaginal secretions without itching (37%), (31.25%), whereas in those with symptoms of copious secretions onlythe corresponding figures (30.17%), (18.51%) were recorded in bothcities respectively (P<0.05). 7 - The maximum infection rate with C. trachomatis in bloody swabs were (40%), (50%) in Kirkuk & Tikrit cities respectively correlated withclinical signs & symptoms. However, maximum infection rate with G. vaginalis was recorded in women with whitish vaginal discharge (60.86%) or yellowish (53.12%) then greenish (52.94%) in Tikrit city in comparison to Kirkuk city. The maximum infection with G. vaginalis were among women with bloody secretion (40%) followed by whitish secretion (30.35%). (P<0.05). 8 - The maximum infection rate with Candida spp. were recorded in women discharge milky secretions (33.33%), (25%) in Kirkuk & Tikrit cities respectively. Infection with N. gonorrhoeae showed different color vaginal discharge (P>0.05).9 - The maximum rate of infections with T. vaginalis (27.43%), (19.69%) were recorded in women whose pH of vaginal secretion range was between (5 - 6). (P> 0.05). The high vaginal swabs have been revealed to be more efficient in diagnosis of vaginal infection with T. vaginalis than endocervical swabs. The maximum rate of infections recorded by these swabs were (22.22%), (19.73%) respectively. The infection rate of pregnant women were (48%), (22.22%) from positive cases in both cities.10 - The maximum rate of infections with T. vaginalis were recorded in women using various contraceptive, women using intrauterine contraceptive device were the highly infected (33.33%), (31.57%) in both cities. P<0.05
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2008
الموضوع العام: علوم الحياة
اسم المشرف: يحيى جرجيس سلمان ابراهيم شعبان داود
اسم المؤلف: برهان احمد محمد علي بيباني

تقييم دور الجين في دم نساء عراقيات مصابات بسرطان الثدي Evaluation The Rule of Mammoglobin A Gene In Blood of Iraqi Women With Breast Cancer

المستخلص: Mammaglobin A is a member of the superfamily of secretoglobins. Its expression is highly specific for mammary tissue and has been shown to be overexpressed in breast tumor tissue, indicating that mammaglobin A might confer a growth advantage to mammaglobin A - expressing tumor cells. The present study was undertaken to develop the mammaglobin A as a serum biomarker for the early detection of breast cancer, and breast cancer patients follow up after treatments. This study had taken ten months, it involved 84 patients with age ranged 15 - 74 years who had diagnosed with either stage I to III breast cancer or benign lesions of breast at certain Iraqi hospitals and breast cancer centers, and a total number of 10 apparently healthy individuals, with comparable age range of patients as control group.Ten ml of venous blood was collected from studied groups in order to be used for measuring estrogen and progesterone levels, Reverse transcription polymerase chain reaction (RT - PCR) test for detection of their serum m - RNA mammaglobin A existence, and serum mammaglobin A levels using Enzyme - Linked ImmunoSorbent Assay (ELISA) technique. Demographic study revealed that most cases enrolled in this study were women within their reproductive age 25 - 44 years old. Distribution of patients according to age group, marital status, and lactation status showed no statistically significant association (P>0.05) with any of these variables, in all comparison.The hormonal study states that elevated levels of estrogen were observed in 52 (62%) patients, also decrease in progesterone levels were observed in 50 (59%) patients out of the 84 cases studied. This indicate a highly significant SUMMARYassociation (p < 0.01) between breast cancer risk, elevated estrogen levels, and decrease in progesterone levels. Mammaglobin A protein was detectable by ELISA in 100% of breast cancer patients (65 case), and not in any benign tumor patients (19 case) nor healthycontrol women (10 females) which indicate the highly specificity of ELISA test (P<0.01) for measuring mammaglobin A levels in patients serum. With high concentration of protein in sera of women with late stage of breastcancer compared with a low protein concentration in sera of women with early stage of breast cancer. By applying a statistical analysis on the result it was found that serum mammaglobin A ELISA test is highly significant (P<0.01) for, differentiation between patients with metastatic breast cancer and patients with early diagnosed breast cancer. In this work, SCGB2A2 gene which coded for mammaglobin A was detected in 64 out of 65 breast cancer cases, but not in the benign or healthy individuals indicating its high specificity as a marker gene (SCGB2A2) for cells derived from mammary glands. A statistical analysis was made for comparison between the three groups, E - Malignant Tumors \ Early Stage, L - Malignant Tumors \ Late Stage, and B - benign, (E, L, and B) which show that the results of molecular study of mammaglobin A detected by RT - PCR is statistically high significance (P<0.001) when used for the differentiation between E group and B group, also between L group and B group. The results showed that mammaglobin A can become an important tool for detecting primary and metastatic breast cancer, and predicting disease outcome.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: امنة نعمة الثويني
اسم المؤلف: نانسي فيصل رشيد

دراسـة بيئيــة للافقريات القاعيـة في هور الدلمج - وسط العراق An Ecological Study of The Benthic Invertebrates In Al Dalmage Marsh - Middle of Iraq

المستخلص: استهدفت الدراسة الحالية التنوع الاحيائي لمجتمع لافقريات القاع في هور الدلمج / وسط العراق فضلا عن بعض الخصائص الفيزياوية والكيمياوية لمياه الهور، اختيرت ست محطات لجمع العينات وذلك للمدة من بداية كانون الثاني وحتى كانون الاول 2013. استخدم تحليل التوافق CCA | Benthic invertebrate diversity on the Al - Dalmage marsh in the middle of Iraq, in addition to the some physical and chemical properties was the goal of the present study, for a period from January to the end of December 2013. Six stations along the eastern side of the marsh were chosen for monthly samples collection.The results showed water temperature ranged from(9 - 34.1)?C. The pH values were neutral but tends to near alkaline and ranged from (7.2 - 9).The Electrical conductivity values varied from (22735 - 2330) ?s/cm, according to the values of salinity (1.5 - 14.6) ‰ the marsh was classified as mesohaline (TDS) which showed high values ranged from (1820 - 17762) mg/L.This study showed that the marsh were well oxygenated due to the dissolved oxygen values which ranged from 7 - 12 mg/L, the study recorded values to BOD5 were ranging from (1.5 - 3)mg/L, and the marsh classified as very hard according to the total hardness values from (6980 - 825) CaCo3 mg/L. The high perecentage of organic matter in the sediment was 2.7 %. The results showed the station were characterized by sediment texture varied from silt clay sand sediment in the stations 1, 4, 5 , silt clay in St(2) and sand silt in St(3), sand clay silt in St(6).A total of 21286 individual benthic invertebrates was sorted, which represents which 78 taxonomic units, including 9 taxa. The Crustacea was the dominate group representing 29% of the total number of benthic invertebrates. It was noted that there were obvious differences in the total number of sorted individuals in which varied in each location and each month. The highest total number recorded in was st (2) was 4360 including 73 taxonomic units, while the highest total number recorded in April was 2624.The results of The relative abundance index showed that the species insects : Polypedilum sp Were more abundant in St(2 - 6), and dominant in St(6), while the Seinura sp were dominant in St(6), while the other taxonomy units varied from less abundant to rare abundant. Also the results of constancy index showed The which were considered "constant " However the other taxonomy units varied from "accessory " and" accidental" taxonomy units in study stations.The Shanon - Weiner index of totall benthic invertebrates varied from (1.2 - 3.8) bit/Ind, The uniformity index of benthic invertebrates varied from(0.7 - 1), these high values indicate that there is no ecological stress on benthic invertebrates in the study area.The Results of Jaccard Presence - community of similarity showed the highest degree of similarity to be 96% which recorded between St(1) and St(2).The values of the index of spcies richness varied from 1.3 - 15 with the greatest values recorded in April while lower values were in October.Six species of Turbellarian Platyhelminthes that were recorded, from which Stenostomum leucops, Stenostomum bryophilum, Stenostomum unicolor, Gyratrix hermaphrodites, Macrostomum sp, Dalyelliidae, which were considered as new records for Iraq, with Gyratrix hermaphrodites as the most abundant species and representing 25% of the total number of Turbellarian. It was noted that there were obvious differences in the total number of sorted individuals which varied in each location and each month, the highest total number recorded in St 3 was 400, while the highest total number recorded in November was (198).The present study result showed that three species of Nematoda were recorded incuding Seinura sp, Alaimus sp, Dorylaimus sp, Seinura sp as the most abundent species representing 84% of the total number of Nematoda. Spatially the highest total number of 590 individuals was recorded in St (4) , while temporally, the highest total number of 366 was recorded in October.One unidentified species of Gastrotricha was recorded for the first time in Iraq, 102 Ind were sorted these were considered "constant" recording to constancy index 63.8% which were sorted from all stations.A total of 2319 individuals of Rotifera was sorted, these were identified as 15 species that included Philodina sp, Rotaria citrinus, Dipleuchlanis sp, Euchlanis sp, Notholca sp, Ttriploeuchlainis sp, Colurella sp, Lepadella sp, Lecane sp, Proales sp, Cephalodela sp, Synchaeta sp, Hexarthra mira, and finally Brachionus sp which was recorded the highest number of individuals 540 individuals.A total of 1888 individual Annelida were sorted representing 12 species including nine species belonging to the family Naididae (three belong to the sub family Naidinae and six belonging to Tubificoid worms), two species belonged to Aleosomatidae including Aeolosoma hemprichi, Aeolosoma variegatum, and finaly one specie belong to Hirudinea which was Glossiphonia heteroclite, with three 3 ind in all study.The family Aleosomatidae recorded the highest percentage of 58% of the total number of Annelida.The sub family Naidinae including Paranais litoralis, Dero (Aulophorus) furcata, Stylaria lactustris.The highest frequency of 23.6% was recorded by P. litoralis.The Tubificoid worms represent six species which were Limnodrlius hoffmeisteri, Limnodrlius profundicola, L. claparedeianus, L udekemianus, Tubifex tubifex, Branchiura sowerbyi. This study also isolated 6238 individual Crustacea, these were identified as 15 species that included five species belonged to Cladocera, four order belong to copepod, which were recorded the highest percentage 30% of the total number of Crustacea, in addition to Macrobrachium nipponense from Decapoda, Sphaeroma annadalei anndalei from Isopoda, Ostracoda, Amphipoda, Conchostracha. It was noted that there was obvious differences in the total number of sorted individuals which varied in each location and each month, The highest total number recorded in st (2) was 1480, while the highest total number of 730 was recorded in April. The study finds 4297 individual insects groups, were sorted represent seven order includes larvae of Ephemeroptera, Trichoptera, Odonata, (Zygoptera, Anisoptera) Diptera (Culicidae, Chironomus sp, Polypedilum sp) Lepidoptera larvae Hemiptera (Corixidae, Nepa cinerea), and adult of Coleoptera (Dysitidae, Hydrophilidae Elmidae, Curculionidae, Coleoptera larvae), the Diptera larvae were recorded the highest percentage 55 % of the total number of insects groups, and Nepa cinerea was considered as new records for Iraq.This study showed two class from Mollusca, 1956 Ind were sorted, including Gastropoda and Pelecyopoda.The Gastropoda which were recorded the highest percentage 94% of the total number of Mollusca, these were identified to physa acuta, Lymnaea sp, Melonoides tuberculata, Melnopsis nodosa, Theodoxus sp, physa acuta which was the greatest percentage 41% of the total number of gastropoda. pelecyopoda which were including Pseudodontopsis euphraticus, Tigridis Unio, Corbicula fluminea, Corbicula fluminali
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: دكتوراه
الموضوع الدقيق: الحيوان - اللافقريات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: هيفاء جواد جوير
اسم المؤلف: ميسون حسن مشجل السراي

دراسة تصنيفية حياتية مقارنة لانواع من الجنس Euphorbia L. (Euphorbiaceae) في العراق A Comparative Biosystematic Study of Some Species of The Genus Euphorbia L. (Euphorbiaceae) In Iraq

المستخلص: تناول البحث الحالي دراسة تصنيفية مقارنة لثلاث وعشرين مرتبة تصنيفية تعود جميعها للجنس Euphorbia L. من العائلةEuphorbiaceae النامية في العراق، تضمنت الجوانب المظهرية العامة والدقيقة ودراسة حبوب اللقاح والمركبات الكيميائية والبيئة والتوزيع الجغرافي فضلا عن ا | The current research deal with twenty - three taxa belong to the genus Euphorbia L., Family Euphorbiaceae grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also studied and the chemical compounds, Ecological and geographical distribution and taxonomic treatment have done.Also botanical key was putted to separate the species. General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens studied, and correct the mistake naming, specimens were identified and reidentified some miss identified specimens in the Iraqi herbaria. A comparative morphological study was undertaken to all plants parts of taxa appeared from habit and duration of taxa to the quantitative and qualitative attributes the members of the vegetative and floral parts included the roots, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying with natural images for different morphological characteristics, as well as the field images. This study showed that all species was herbs, shrubs and succulent and semisucculent annual and perinnal, the major taxonomic importance was for flowering parts includeing the specific Inflorescence, installation of a cupped and its regularity on the branches of the plant, glands forms, numbers, presence or lack of appendages, bractes and bracteoles shapes, dimensions and color fruits shape, indumentum and seeds forms, dimension and her surface sculpturing; and the presence or lack of caruncle, as well as the qualities of roots, stems and leaves, and the morphological characteristics of all plant parts mentioned above have a taxonomic importance in identification and separation of the studied taxa. The study of the micromorphological characters of hairs, leaves epidermal cells and the stomatal complex which included anomocytic, paracytic, anisocytic and tetracytic, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa.The pollen grains have a taxonomic importance because their different in shape, dimensions of polar and equatorial view, the wall thickness and their ornamentation. They are mostly of Tricolporate except two species were Hexacolporate, also the differences in their shape in the polar and equatorial view.They were spheroidal, ovoid and oblate - spheroidal in the polar view and oblate, spheroidal, subprolate and oblate - spheroidal in the equatorial view.The ornomentation they were reticulate, microreticulate and foveoate.The chemical study also importance in separation of the genus taxa four compounds Flavonoids diagnosed in all the studing species but different in concentrations, by using HPLC, they are Myriscetin, Quercetin, Kaempferol and Coumarin esculetin and this confirms that there are a common evolutionary link between the species in terms of their chemical properties, which suggests belonging species to the genus Euphorbia.Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical districts. the species of Euphorbia were distributed in all Iraqi districts especially in LCA, MSU, MRO, and DWD and some species were farmed in gardens and nurseries either to the beauty of their bractes colors or because their acceleration to the environmental changes such as succulent species, As well as wild species were spread in mountainous areas, oak, nuts forests, in orchards, cultivated fields, flat plains and desert areas in loam soils, clay, gravel, and sand. Through this study some new taxa where founded in Iraq and they are E. cyparissias Kunth ; E. maculata L. ; E. serpens L. ; E. tirucalli L., and this study suggests to recorded them for the first time in Iraq.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عذية ناهي سلمان المشهداني
اسم المؤلف: ازهار طاهر صليبي

دراسة بيئية وسكانية للقوقع الارضي Candidula gigaxii (L.Pfeiffer, 1850) (Pulmonata : Hygromiidae) في مواقع مختارة من محافظة بغداد Ecological And Population Study of The Land Snail Candidula Gigaxii (L.Pfeiffer, 1850) (Pulmonata : Hygromiidae) In Selected Locations From Baghdad Province

المستخلص: Few studies on terrestrial snails in general, and garden snails in particular, existin Iraq. The group being important apparently for its wide - spread distribution in various habitats. One species of this group which has been previously recorded in Baghdad, is the garden snail Candidula gigaxii, class : Pulmonata, family : Hygromiidae.Available samples of the land snail C.gigaxii were collected from four locations within Baghdad province; a Taji, the Army Canal area, the Zafaraniyah, and Al - Jadiriya district, the study period of June 2013 to May of 2014.Environmental measurements were recordedat each site. Results showed that air temperature range was 10 - 34? C, soil temperature range was 5 - 26? C, pH range of the soil was between 4 - 8.9 and the content of the soil organic matter was between 0.3 - 2.0%.Snail samples were divided into seven size - classes according to the dimension of the shell and these were <2, 2 - 4, 4 - 6, 6 - 8, 8 - 10, 10 - 12, >12 mmPercentages of each size class were calculated. Having measured shell dimensions, shell growth index (Ia) was also calculated. The range of growth index value for this snail was 1.9 - 3.86 where the highest value was recorded at the Taji site in January 2014 which was probably attributed to low temperature range and high soil moisture and organic matter content.Results also showed that C. gigaxii favored soils with moderately high pH values and rich in organic content. Soil moisture appeared to be a limiting factor for growth; and a loamy soil texture for existence of the studied species. Average highest population density of living snails and empty shells were 44 and 32 individuals / m2 respectively. The overall population density was also calculated for the purpose of obtaining realistic population density of the species.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عماد الدين عبد الهادي المختار
اسم المؤلف: بشرى محمد كاظم ديوان البيضاني

دراسة نوعية مياه خزان الحبانية موقعيا والاستشعار عن بعد Study of The Water Quality In Alhabbaniyah Reservoir In Situ And Remote Sensing

الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: البيئة المائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: عادل مشعان ربيع
اسم المؤلف: احمد عطا الله حسن الفهداوي

العلاقة بين مؤشر الموت الخلـوي المبرمج (ربيطة فاس الذائبة) وقلة النطاف عند الرجال Correlation Between Apoptotic Marker (Sfas Ligand) And Oligozoospermia In Men

المستخلص: The Fibroblast associated (Fas) system in the testes has been identified as a key regulator of apoptosis, a process that greatly influences the germ cell population of the testes. and the soluble Fas Ligand (sFasL) may be a competitive inhibitor to induce apopto¬sis of the Fas baring cells. Therefore, this study measures the level of sFasL in the semen of oligozoospermic men evaluating the association between seminal plasma sFasL and spermatogenesis.A total 58 oligozoospermic men and 29 normozoospermic volunteers were included in this study. They were attending the Infertility Clinic of the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, AL - Nahrain University\ Baghdad. Their age mean was 31.77± 5.48 years and a median of 30 years. Semen samples were evaluated according to World Health Organization 2010 standard parameters. The Seminal plasma was separated from the cellular elements after centrifugation and the supernatant was transferred to plane tubes and frozen at - 20 ?C till using for measurement of sFasL. These patients were classified into patients with severe oligozoospermia (sperm concentration ?5 million / ml) which include 29 patients, and patients with mild and moderate oligozoospermia (sperm concentration ranging between 5 - 15 million / ml) which includes 29 patients as well. The control group comprised of 29 men with normospermic parameters according to WHO 2010 standard (Normal volunteers). The sFasL was measured using ELISA enzyme immunoassay for quantitative determination of sFasL Kit. Data were analyzed statistically using Analysis of variance (ANOVA), Least Significant Difference (LSD) and Correlation Coefficient (r).The sFasL level was found significantly higher (P< 0.01) in seminal plasma of oligozoospermic men, with weak correlation of the level of sFasL with the degree of severity of oligozoospermia. Based on the results of this study, it was concluded that sFasL is a novel marker found in the seminal plasma of oligozoospermic men. It's level was higher in cases of oligozoospermia regardless of its severity.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: صباح ناصر العلوجي انعم رشيد الصالحي
اسم المؤلف: احمد حسين علي الزاملي

تكامل جرعة منخفضة من مبيد الترفلان مع مخلفات زهرة الشمس في مكافحة الادغال وتحسين نمو محصول الماش لتقليل الاعتماد على المبيدات والحد من مخاطرها Integration of Reduced Dose of Trifluralin Herbicide With Sunflower Residues For Weed Control In Mungbean Field

المستخلص: Two field experiments, several greenhouse and laboratory experiments were conducted during the course of study to evaluate the allelopathic potential of two sunflower (Helianthus annuus L.) cultivars on companion weeds and weeds grown in mungbean (Vigna radiata L. R. Wilczek) field alone and in combination with reduced rate herbicide, and to determine the chemical and genetical bases of allelopathic traits in the test cultivars. The aim of the first experiment was to test whether the variation in weed growth between the sunflower cultivars (Shumoose and Sin Altheeb), which was observed in the field, was due to differences in their allelopathic potential. Results showed the ability of both cultivars to reduce weed population and biomass with the superiority of Shumoose cultivar in suppression of weed density at 60 and 120 days after sowing compared to Sin Altheeb. Weed biomass was significantly reduced up to 90 and 71% by Shumoose and Sin Altheeb cultivars, respectively. Stair case experiment indicated that root exudates of Shumoose cultivar showed more suppression to weeds than Sin Altheeb giving additional evidence for the superiority of Shumoose cultivar in its allelopathic weed suppression. Chemical analysis by High performance liquid chromatography indicated the presence of 9 compounds in root exudates of Sin Altheeb and 8 compounds in Shumoose, and all are Phenolic in nature. However, total of the isolated phenolics was doubled in Shumoose than in Sin Altheeb. Most of the isolated chemicals are reported to have inhibiting ability for germination and growth of plants, including weeds. Second field experiment was undertaken to explore the response of weeds and mungbean crop to soil incorporated with sunflower residues in combination with lower rate of a pre - plant herbicide (trifluralin). Sunflower residues of the two cultivars Shumoose and Sin Altheeb at 3.2 and 6.4 t ha - 1 were used either alone or in combination with trifluralin at 1.2 L ha - 1 (50% of label rate). Weedy check (control), trifluralin at full label rate (2.4 L ha - 1), and weed free treatments were also included for comparison. Plots treated with 50% of label rate of herbicide and amended with sunflower residues of cultivar Sin Altheeb recorded least weed density and dry biomass and this suppression was much greater than the residue treatments alone and more than cultivar Shumoose. Application of herbicide at 50% rate in plots amended with Sin Altheeb residue resulted yield 64 % more than with the label rate of herbicide treatment. Chromatographic analysis of sunflower amended field soil revealed the presence of several potent allelopathic compounds in the residues of both cultivars with greater quantity (355.5 µg/ml) in Sin Altheeb than in Shumoose (250.9 µg/ml). Dynamics of release, decomposition and degradation of allelochemicals into the soil showed that different compounds exhibited differential behavior for these processes. Overall quantity of allelochemicals started to increase after 2 weeks of decomposition and peaked at 4 week of decomposition (180.1 ppm) then declined sharply in their quantities thereafter. Periods indicating higher quantities of total phenolics as shown by chromatographic analysis was coincided with the periods in which higher suppressive activity against weeds grown under field conditions. Bioassay of sunflower residues decomposed in soil at different times on Echinochloa colonum L, one of the weeds dominated the mungbean field, revealed that residues of both cultivars suppressed growth of E. colonum weed. The phytotoxicity started after 2 weeks and persisted for 8 weeks. However, Sin Altheeb residues showed greater inhibition than Shumoose cultivar at the last three decomposition periods. Shumoose residues recorded appreciable reduction to the growth of test weed compared to Sin Altheeb. The effect of residues of both cultivars on chlorophyll content and ions uptake in Amaranthus retroflexus L. weed revealed that residues of both species reduced chlorophyll content of leaves and the reduction increased with the increased residue rate. Sin Altheeb cultivar residues were more inhibitory than Shumoose at the lower residue rate. In the same weed, ions uptake was significantly averted by the residues of both cultivars. P and K was significantly increased over control, while Ca, Mg an Fe were found to be decreased over control due to application of sunflower residues in soil at 8 g per kg soil. N was the only test element that did not significantly influence by the residue treatments. No significant differences were found in the test ion uptake between the test sunflower cultivars. Results of using RAPD technique on the sunflower genomic DNA revealed that all the 19 primers used in this study scored different amplification monomorphic and polymorphic bands in the tested genotypes with 12 primers generated polymorphic bands. These bands present in one cultivar and absent in another. They could be responsible for allelopathic trait or secondary compounds associated with this phenomenon. Further work is recommended to analyze the sequence of this band to find out whether it is related to allelopathic trait or not.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: دكتوراه
الموضوع الدقيق: النبات - الفسلجة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: اﺑﺮﺍﻫﻴﻢ ﺷﻌﺒﺎﻥ ﺍﻟﺴﻌﺪﺍﻭﻱ
اسم المؤلف: اﺭﻭﻯ ﻋﺒﺪ ﺍﻟﻜﺮﻳﻢ ﺗﻮﻓﻴﻖ

التنميط الجيني لفايروس التهاب الكبد نوع اي لمرضى الاصابة الحادة في محافظة المثنى - العراق Genotyping of Hepatitis E Virus Among Patients With Acute Infection In Al - Muthanna Province / Iraq

المستخلص: Hepatitis E Virus (HEV) infection is found worldwide. It is responsible for large outbreaks in East and South Asia. This study was carried out to identify the distribution of acute HEV infection in AlMuthanna province and then determine the genotypes responsible for the disease spreading. This is the first investigation about the occurrence of hepatitis E genotypes in Al - Muthanna province patients. The study included 270 jaundiced patients of both sexes From AlMuthanna province which included out patients in public health laboratory in Samawah city, for the period from October 2013 to June 2014. All these patients were tested for anti - HEV IgM antibodies by using enzyme linked immunosorbent assay (ELISA). In Al - Muthanna province, 72 patients (26.66 %) out of 270 patients showed positive results for anti - HEV IgM antibodies, and all those patients were tested for confirmatory test at central public health laboratories (CPHL) in Baghdad province. Those patients consist of 45 females and 27 males with age ranged between (4 - 74) years old, were all negative for routinely screened markers of Hepatitis A, Hepatitis B and Hepatitis C, Ten normal healthy individuals, used as normal control in this study. This study showed that the HEV IgM is more common among younger age group (15 - 24), with a percentage of) 41.67%(, and it was higher in females (63%) than in males (37%). Highly significant differences (p ? 0.01) appeared among age groups. The levels of liver function enzymes demonstrated significant differences (p ? 0.05) in HEV patients as compared with healthy controls. The levels of Total Serum Bilirubin (TSB) were (23.41 ± 12.38) mg/dl, Serum Aspartate transaminase (SAST) were (887.17 ± 9.87) U/l, Serum Alanine transaminase (SALT) were (859.91 ± 13.28) U/l, and the levels of Serum Alkaline phosphatase (SALP) were (206.56 ± 5.04) U/l higher than healthy controls (0.884 ± 0.05) mg/dl, (37.27 ± 4.58) U/l, (34.14 ± 7.63) U/l, (48.09 ± 15.42) U/l respectively. RNA was extracted from sera of positive anti - HEV IgM antibodies by using a QIAamp® Viral RNA Mini Kit. Nanodrop and Quantus™ Fluorometer instruments were used to determine the RNA yield and purity according to the standard kit protocol. High levels of RNA concentration were measured by Nanodrop and Quantus™ Fluorometer. Forty - six samples have high RNA concentration out of 72 samples were detected for genotypes I, III & IV by using Applied Biosystems Real time PCR 7500 machine. In this study, HEV type I and III were detected in 23 samples out of 46 samples by using Real Time PCR systems. Ten samples were positive for this test. Thirteen samples (56.52%) were negative for this test. The distribution percentage of genotypes I and III was (43.48%). The results appeared significant difference (P ? 0.05). Also by using the same technique (Real time PCR systems) the HEV type IV was detected in 23 samples out of 46 samples. Which included, 13 samples that recorded negative results by I & III genotypes kit and only 2 samples from it recorded positive results and 11 samples recorded negative result for HEV genotype IV. From other 10 samples, only 3 samples recorded positive result and 7 samples recorded negative result for HEV genotype IV. In a total, 5 samples were positive for HEV genotype IV and 18 samples (78.26%) were negative. So that the distribution percentage of genotype IV was (21.74%). In this study, the samples that showed negative results in both kits have not been tested for other genotypes. This study indicated that the acute HEV infection is highly endemic in Al - Muthanna province in Iraq. Genotypes I & III were the most distribution than genotype IV in patients from Al - Muthanna province in Iraq. These results suggest that the genotypes I & III are the main causative agents of sporadic HEV infection in Al - Muthanna province
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: رغد حربي مهدي العزاوي
اسم المؤلف: ازهار صبري مسلم الذهبي

تاثير بعض العوامل البيئية في مجتمع الهائمات القشرية لنهر دجلة وجزيرة بغداد السياحية - العراق The Effect of Some Environmental Factors In Crustaceans Plankton Community of The Tigris River And The Island of Baghdad Tourist - Iraq

المستخلص: The present study was conducted to investigate the effect of the water body type on the density of crustaceans zooplankton of the cortical Cladocera and two orders of copepod (Cyclopoida, Calanoida), addition to the physical and chemical effect of lake of Baghdad Tourist Island and Tigris river within the water of the lake near the site and electric and know the effect these characterties on density of crustaceans Zooplankton and for doing this study. We choose five places, tow on Tigris river and three places on the Island in AI - Fahhama in north of Baghdad The samples of water were collected every 15 days all these stations from beginning of December 2013 to May 2014. This study had corrected the temperature of air and water and the pH, electrical conductivity. and Dissolved oxygen, BOD5, NO?¯, PO?¯ and the density of Crastaceans Zooplankton. This study showed that the temperature of the air was nearly in the river and the lake and that because of their being in the same geographs area.The temperature was between (16°c - 34°c)in Tigris while it was (11.9°c - 33°c)in the lake Hydrogen Ions, it was (7.3 - 7.9) in the river and (8.18.8)in the lake because that were a lot of water plants and phytoplankton. This study shows that water of the river and the lake in the areas of study have a good of air and that was shown by recording the heigh values of oxygen. The values of dissolved oxygen (DO) was between (8.11 - 11.5) mg\L in river between (7.9111.37)mg\L in lake and showed from the study that the values were few. It was less than 5mg\L it was (0.33 - 2.6) mg\Lin the river and (0.64 - 3.55) mg\L in the lake. The values electrical conductivity of water in all the placed the study in the permissible range the water, it isn't over than 1500?s\cm. The electrical conductively records were between (851 - 1184) ?s\cm in the river, and it was (755 1270) ?s\cm in the lake. From the other hand nutrients, the phosphor records between (0.025 - 0.0563)mg\Lin the river and in the lake between(0.00310.0429)mg\L The Nitrate records its values highs in the month of winter because of the rain and it was increased in the river, it was (0.345 - 0.9135)mg\L compare with the lake, it was (0.028 - 0.626)mg\L in the places of the study that were appearance of two clear increases in the density of Zooplankton one was in winter and the other was spring.The density of zooplankton in the lake much more than it was in the river. The density of the zooplankton belong to Cladocera between (0.019 - 0.307) ind.\L in the lake and the density of the Calnoida was between (0.003 - 0.065)ind. \L in the river and (0.0078 - 3.37) ind. \L in the lake. The results shows differences if there are changes in places and month on most of physical and chemical biological characteristic the measured during the period of this study. The kind of water body effects on most characteristics and soon the density of crustaceans zooplankton.the results of the study mention the binding morale relation between the density of zooplankton the values of electrical conductivity and value of BOD(Biological Oxygen Demand) while there isn't any morale relation between the density of zooplankton and the value of Hydrogen Ion and Nitrate and dissolved oxygen that because the value of these changes was near to be equal in all the places that eayses the reduce of its effective on the average of density of these organism. The study shows that Baghdad lake have the environmental ingredients makes the lake natural protected to forming and breeding a fish in the lakes in addition that it is a place for Tourism.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الحيوان - اللافقريات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: صباح فرج عبد الاحد
اسم المؤلف: اعتدال فليح حسن العامري

تاثير عصارة نبات البروكلي في متلازمة تعدد الاكياس المبيضية المستحدثة في الجرذ الابيض Effect of Broccoli Plant Juice On Induced Polycystic Ovarian Syndrome In Albino Rats

المستخلص: The goal of present study was to investigate the benefits of broccoli as anti androgenic plant in protection and treatment for reproductive hormonal and metabolic disturbance, which combined with polycystic ovarian syndrome (PCOS). Three experiments for 3 PCOS models were done. First Experiment : The prepubertal female rats at 21 days old, were injected with Testosterone (T) subcutaneously for 39 days (first model). The females of this experiment were divided into 6 groups (8 rats each) as follows : Group 1 was injected with sesame oil. Group 2 was injected with T hormone. Group 3 was injected with T hormone with orally gavaged of broccoli juice. Group 4 was injected with sesame oil, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 5 was injected with T hormone, and at the end of the last injection was orally gavaged with distilled water for 30 days. Group 6 was injected with T hormone, and at the end of the last injection was orally gavaged with broccoli juice for 30 days. Second Experiment : The prepubertal female rats at 21 days old, were injected with Letrozole (L) subcutaneously for 39 days (second model).The females of this experiment were divided into 6 groups with the same style of the first experiment. Third Experiment : it was achieved by the injection of pregnant rats prenatally (days 16 to 19 of gestation) with 1 mg of T hormone subcutaneously (third model), and then the female offspring were divided into groups (8 rats each) as follow : The first offspring group : The female rats offspring at 21 days old (their mothers were injected with sesame oil from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with distilled water : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with distilled water until 60 days of age. The second offspring group\ gavaged with broccoli juice : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation) were orally gavaged with broccoli juice until 60 days of age. The third offspring group : The female rats offspring at 21 days old (their mothers were injected with T hormone from 16th to 19th day of gestation and orally gavaged with broccoli juice) were orally gavaged with distilled water until 60 days of age. Females of all groups were sacrificed after 24 hours from the last treatment to undertake weight, histological, hormonal and metabolic study for each group. the results of present study showed the symptoms that reported PCOS of the three female experiments such as ovarian cyst, hyperandrogenemia, hormonal and metabolic disturbance, etc. Also a well improvement has been noticed in all syndrome symptoms on females gavaged with broccoli juice for all three rats PCOS experiments, these results indicate that broccoli juice has got a protective and curative role against this syndrome.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الحيوان - الفسلجة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: نهلة جاسم محمد الشاهري
اسم المؤلف: رقية احمد صالح

التحري الجزيئي عن النمط الثالث لداء السكري البادئ عند النضج (MODY 3) في مرضى السكري العراقيين Molecular Investigation of Maturity Onset Diabetes of The Young Type 3 (MODY 3) In Iraqi Diabetic Patients

المستخلص: شملت الدراسة 63 مريضا مصابا بداء السكري غير المناعي المنشا في اعمار مبكرة من ذوي التاريخ العائلي بالاصابة بمرض السكري ممن لايعانون البدانة الذين تم اختيارهم من مرضى السكري من مراجعي المركز التخصصي لامراض الغدد الصم والسكري في الرصافة/ بغداد للفترة من ايار | This study included 63 non obese patients having early onset of non immunogenic diabetes with strong family history of diabetes selected from diabetic patients attended the specialized center of endocrinology and diabetes at Alrusafa/ Baghdad during period from the begging of May 2011 till the end of February 2013. The demographic study revealed that there is no association between the disease incidence with neither the gender nor the blood group. But there is a significant difference in the distribution of the patients according to age of the first diagnosis and gender, as the study showed that majority of male patients (53.846%) were first diagnosed with hyperglycemia below the age of 20 while the majority of females (72.972%) were first diagnosed with hyperglycemia after the age of 20.The distribution of patients according to the type of treatment also reveled association between the gender and both age of diagnosis and type of treatment of the hyperglycemia as the study showed that there is a higher percent of male patients (80.768%) using insulin at the beginning of their diagnosis with hyperglycemia or transferred later to insulin than those of females (67.565%).The present study reported for the first time in Iraq the presence of monogenic diabetes (maturity onset diabetes of the young type 3) as major cause of diabetes within non obese diabetic patients' early onset of non immunogenic diabetes with strong family history of diabetes. The sequence analysis of the hepatocyte nuclear factor 1? gene showed that Iraqis have mutational hot spot at exons 3 and 4 of this gene responsible for maturity onset diabetes of the young type 3, and that mutations in the promoter region or exons 5, 6, 8 and 9 are a rare cause of diabetes.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الدرجة: دكتوراه
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: نورية عبد الحسين علي
اسم المؤلف: اسراء عدنان ابراهيم البغدادي

دراسات وراثية خلوية ومظهرية لثلاثة انواع من شبوطيات المياه العذبة العراقية (Barbus luteus, Cyprinion macrostomus, Chondrostoma regius) Three Species of Iraqi Freshwater Cyprinidaes (Barbus Luteus, Cyprinion Macrostomus, Chondrostoma Regius)

المستخلص: هدفت الدراسة الحالية التعرف على الطراز الكروموسومي ونظام تحديد الجنس والتمييز بين الجنسين مظهريا في ثلاثة انواع من اسماك المياه العذبة العراقية ممثلة بسمكة الحمري Barbus luteus والبنيني كبير الفم macrostomus Cyprinionوالبلعوط الملوكي Chondrostoma regius | These present studies were carried out to determine the karyotypes & the system of sex determination between the two sexes as well as the sexual morphological characteristics of three species of Iraqi fresh water fish Barbus luteus, Cyprinion macrostomus, & Chondrostoma regius that were fished from Tigris river at Baghdad during 2005. The cytogenetic studies revealed that the chromosome number of Barbus luteus is 2n = 148 & the karyotype of males is (80m + 52sm + 16st) while the females has (80m + 51sm + 17st). The chromosome number of Cyprinion macrostomus is 2n = 50 the males has (6m + 24sm + 12st + 8t) while the females has (6m + 23sm + 13st + 8t). The chromosome number of Chondrostoma regius is 2n = 48 and the karyotype is (14m + 30sm + 4st). Sex chromosomes were identified in Barbus luteus & in Cypinion macrostomus for the first time , the sex is determined by ZZ / ZW system, the female was considered as heterogametic (ZW) while the male was considered as homogametic (ZZ) , & the chromosome Z was represented as submetacentric chromosome & chromosome W as small subtelocentric chromosome. In spite of the difference in their chromosome number , the ratio between red blood cell diameter & it's nucleus was approximated in these species. The differences among these species in both of the diameter of cell and the nucleus didn't match with the differences at the polyploidy levels or with the difference in chromosome number within the same level. The liver & intestine were recommended as new sources for chromosomal studies , because of the good ratio of dividing cells (71.4 % & 58.2 %) respectively from the whole dividing cells in kidney that was considered as a control. The crude aqueous extract of fresh convolvulus arvensis leaves showed good effect in arresting the cell division of fish at metaphase , the concentration (0.4, 0.6, 0.8, ) mg / gm arrested kidney cells of carp fish at the metaphase in ratios 71.1 % , 88.1 % , 94 % respectively from the colchicines treated kidney cells (control). Positive significance correlation was found (r = 0.9518) between the percentages of arrested cells at metaphase & the concentrations of crude extract. Comparison between the females and males of the three species revealed that in Barbus luteus the distance from head edge to the anal orifice & from head edge to the dorsal fin were larger in males than females. The females have dark colored fins with reddish ventral aspect of head & body in comparison with the males during the whole months of study. So , they were considered as permanently sexual dimorphism characteristics. But , during the spawning seasons (May & June ) a more brighting color of male with reddish coloration of both sides of body were noticed in comparison with female with a golden orange corneal coloration in both sexes. Thus, they were considered as temporary sexual dimorphism characteristics. In Cyprinion macrostomus the distance from the head edge to the anal orifice was larger in females than males. The colors of anal & caudal fins were darker in females than in males during the whole monthes of study.So , they were considered as perminantly sexual dimorphism characteristics. While, during the spawning seasons ( May , June , July & August) , the gill operculum was of orange color only in male that extend to the end of pectoral fin. So , this was considered as a temporary sexual dimorphism characteristic. In Chondrostoma regius the distance from the head edge to the dorsal fin & from the dorsal to caudal fin as well as the pectoral fin base length were larger in females than in males during the whole months of study it was considered that they are permanently sexual dimorphism characteristics. Differential characteristics were not noticed during the spawning season (February & March). The permanently sexual dimorphism & the general characteristics were not affected by the differences of standard length , weight , age & season , because they were observed in fishes of different lengths , weights & ages & during the months of study 2005. But , the seasons affect the development of temporary characteristics in Barbus luteus & Cyprinion macrostomus , because these characteristics were observed during the spawning season only. Aging of Barbus luteus was accompanied by growth increase as indicated by the mean of standard length during May , July, August, September & December & by the mean of weight during May , August, September, October & December. A positive correlation was found between standard length & weight during the months of study , with the exception of January. While aging of Cyprinion macrostomus & Chondrostoma regius was not accompanied by growth increase as indicated by the means of standard length & weight , but a positive correlation between standard length & weight was found during the studied months. Same growth type were observed in the three species of fish , it was allometric in two sexes.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الحيوان - الوراثة
السنة: 2008
الموضوع العام: علوم الحياة
اسم المشرف: ندى عبد المجيد الانصاري طه ياسين الدوري
اسم المؤلف: اسماء سامي ابراهيم

دراسة تصنيفية مظهرية لبعض انواع عائلة قفازات الضفادع من رتبة متشابهة الاجنحة Homoptera : Cercopidae من بعض محافظات العراق Taxonomic Morphological Study of Some Species of Froghoppers, (Homoptera : Cercopidae) From Some Iraqi Governorates

المستخلص: تضمن البحث دراسة تصنيفية مظهرية لخمسة عشر نوعا تابعة لاربعة اجناس لعويلتيCercopinae، Aphrophorinae لعائلةCercopidaeمن رتبة متشابهة الاجنحة Homoptera جمعت من مناطق مختلفة في العراق تم الاعتماد على النوعCercopis vulnerata(Rossi, 1807) نموذجا للعائلة.وهذه ا | The present study comprisers taxonomic morphological of 15 species belong to four genera of the two subfamilies Cercopinae and Aphrophorinae, belong to the family Cercopidae of the order Homoptera, it has been regarded the speciesCercopis vulnerata(Rossi, 1807) as typical sample of the families.The species studied in were : Family : Cercopidae West wood, 1838Sub family : Cercopinae West wood, 1838.Genus : Cercopis Fabricius, 1775.**Cercopis vulnerata (Rossi, 1807)Sub family : Aphrophorinae Amyot&Serville, 1843.Genus : Aphrophora Germar, 1821.**Aphrophora alni(Falle'n, 1805).**Aphrophora major (Uhler, 1896).**Aphrophora oblique(Uhler, 1896).**Aphrophora salicina(Goeze, 1778)**Aphrophora straminae(Kato, 1932).**Aphrophora forneri(Haupt, 1919).**Aphrophora intermedia (Uhler, 1896).*Aphrophora triticasp.nov.*Aphrophora asadisp.nov.Genus : Phileanus Stal, 1864.Phileanus spumarius (Linnaeus) 1758.Genus : Neophileanus Haupt, 1935.** Neophileanus lineatus(Linnaeus, 1758).**Neophileanus campestris(Falle'n, 1805).**Neophileanus longiceps(Puton, 1895).**Neophileanus exclamationis(Thunberg, 1874). *New species for the science.**New recorded to the Iraq insect fauna of Homoptera.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: دكتوراه
الموضوع الدقيق: الحيوان - الحشرات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: حسن سعيد جاسم الاسدي
اسم المؤلف: حلا تايه عمران

التحري عن التشوهات الكروموسومية وطفرات المورث FLT3/ITD في مرضى ابيضاض الدم النخاعي الحاد Screening For Chromosomal Aberrations And Gene Mutations FLT3/ITD In Patients With Acute Myeloid Leukemia

المستخلص: صممت الدراسة الحالية من اجل تسليط الضوء على تاثيرات مرض ابيضاض الدم ((AMLفي بعض معايير الوراثة الخلوية والجزيئية الحاصلة في كريات الدم البيضاء في ثلاثة حالات للمرضى اشتملت على مرحلة ما قبل العلاج, في اثناء العلاج وبعد العلاج الكيميائي. كما شملت الدراسة ا | The present study was designed to shed light on the cytogenetic and molecular effects caused by acute myeloid leukemia (AML) pathogenesis in three stages before, during and after treatment with chemotherapy (in vitro) in lymphocytes. It was also aimed to investigate chromosomal aberrations, micronucleus formation and FLT3/ITD point mutations in CN - AML region 100 - 300 bp compared with healthy control. The study was comprised of forty - seven AML Iraqi patients their ages ranged between 2.5 - 81 years. It included twenty females and twenty seven males compared with twenty - six healthy control. The following results were obtained : AML is most common leukemia in adults and uncommon in children. It was found that 46.8% patients suffer from AML, who were under 15 years old, while 19.15% patients their age ranged between 16 - 30 years; 19.15% of patients their age were more than 45 years and 14.9% of patients their age ranged between 31 - 45 years of the total AML patients. AML is more common in males than females. The percentages of females and males were 42.5% and 57.5% respectively representing 1.35 : 1.00 male : female ratio. Two AML patients 5.3% have diploid cells when examined under light microscope. The highest mitotic index was 7.498±1.7 occurred in patients before taking treatment while recorded 6.784±4.5 during treatment and 7.000±2.5 after treatment. Nuclear anomalies such as nucleoplasmic bridges and nuclear buds were observed in AML patients, Micronucleus mean values recorded 0.033±0.018, 0.020±0.00015 and 0.036±0.01for AML patients before, during and after treatment respectively when compared with the control which recorded 0.002±0.00002. Nuclear division index (NDI) means before, during and after treatment of AML patients were 1.658±0.2, 1.000±0.022 and 1.424±0.19 respectively. Significant differences occurred among the three groups when compared with the control group (1.282±0.09). Extracted DNA from 30 AML patients was amplified by PCR to obtain FLT3/ITD gene from exon 11 to intron 12 and ten of them sent for sequencing. Two patients showed larger bands (470 and 460) bp when compared with wild type (330) bp. Among six patients, three displayed point mutations of deletion and substitution while others were normal since no mutations were detected. The percentages of mutation types were substitution 57.78% and deletion 22.2%. The effect of mutations was missense mutation (55.54%), deletion (22.22%) and nonsense (22.22%). It has been concluded from the current work that AML is more common in adult males, patients suffer from AML exhibited high MI, NDI, MN and point mutations are present in such patients including deletion and substitution causing missense, nonsense and deletion.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عبد الحسين مويت الفيصل
اسم المؤلف: سمارة كاظم محمد

تقييم الواقع البيئي لمعمل بابل /2 للبطاريات Assessment Study For The Environmental Situation of Babylon\2 Batteries Plant

المستخلص: In this study was investigated the quantity and quality of industrial water from (Babil/2 batteries plant in Baghdad) and estimate the amount of air pollutants for some factory sections and estimate the amount of solid waste resulting from the industry and study the impact of these pollutants on the environment and public health.The first target of this study is investigated the physical and chemical characteristics of the discharged water from sections shipping and ficus where the sample water collocated was two samples per month for 6 month was taking during period from Novembers 2013 to May 2014. Some measurements were conducted laboratory and others on site, the result showing the following values of the variables rates for the shipping and ficus sections before treatment and respectivelyResults of the study showed that there is a significant increase inThe concentrations in sulfate and TDS, heavy metal When reached after treatment for the same variables and respectivelypH 9.9, EC 1524.9 ?s/cm, TSS 166.4mg/l, TDS 611.7mg/l, Cl - 64.46mg/l, (SO4)+2 486.8mg/l, BOD 49.2mg/l, COD 220.4mg/l, Pb+2 0.55mg/l, Zn+2 0.09mg/l, Cd+2 0.01mg/lpH 8.2, EC 1191.6?s/cm, TSS 68.1mg/l, TDS 927.6mg/l, Cl - 76.95mg/l (SO4)+2, 722.6mg/l, BOD 4.4mg/l, COD 29.2mg/l, Pb+2 0.04mg/l, Zn+2 0.03mg/l, Cd+2 0.01mg/lFrom the results of the study showed that there is a no significant increase in the concentrations in sulfate and TDS, but increases slowly. In the water of charging department reached the concentration of same variables and respectivelyThe second aims of this study a steamed the concentration and rate of air pollution (Total Suspended Particles, heavy metal, Oxides and gases) collected the sample of outstanding minutes analysis made to know and identify the concentration of heavy metal for same factory section and compared those with local and global determinants also the concentration of air emitted was measured.Results of the study showed that there is a significant increase in the concentrations of the outstanding minutes compared to the allowable limits as it reached the highest concentration in the Department of Shipping 3313.46?g/m3. The lowest concentration was in the section of the Aggregation 0.8155?g/m3. Where the concentration of heavy metals, especially lead element exceeded the permissible limits as it reached the highest concentration in Ficus section 3.53?g/m3 the lowest concentration in the assembly department which is 0.36 ?g/m3 concentration of CO gas in the department of Ficus, Shipping, assembly, queries reachedpH 1.6, EC 12058?s/cm, TSS 148.3mg/l, TDS 10122mg/l, Cl - 76mg/l(SO4) +2 3838mg/l, BOD 23.55mg/l, COD 203.5mg/l, Pb+20.55mg/l Zn+20.46mg/l, Cd+2 0.022mg/lrespectively.(21.7, 3.9, 2.44, 2.84?g/m3), the concentration of CO2 was 231.8, 187.6, 219.4, 225.4?g/m3.In the department of Ficus, Shipping, assembly, respectively queries either NO2 was 0.12?g/m3 in sections of Ficus, Shipping and queries, 0.16?g/m3 in the assembly department. The H2S appeared only in sections Ficus, Shipping with concentration 0.02?g/m3, 0.18?g/m3 respectively, either gas SO2 only appeared in the shipping department and was only 0.01 ?g/m3.The last part of these study was to calculate the amount of solid waste for a period of study in terms of total solid waste generated by the plant was 37.004 tons for eight months and included this waste pastes lead and lead plates and scrap processing units either the amount of recycling of batteries that are broken in the foundry of lead in Khan Dhari amounted to 354.832 tons for eight months, and the output of 181.1 tons. In this study we foxed on the problems in these factory that effected the process of industry and we found liquid wastes in the factory is not efficient in reducing the concentrations of all the variables treatment and gaseous emissions generated in the factory, there are no have any significant treatment of solid waste recycled and are utilized as it emerged that the Babil / 2 battery plant on a large degree of pollution sulfate and TDS with regard to liquid waste. The minutes and the outstanding lead with respect to gaseous pollutants to the current study that recommended a set of recommendations that would work to eliminate or minimize the causes of pollution and thus access to good results.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: البيئة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: سهير ازهر الكاظمي
اسم المؤلف: منال مالك سعدون الموسوي

الكشف عن بعض عوامل البيئة الداخلية للخلايا السرطانية للنساء العراقيات المصابات بسرطان الثدي Detection of Some Microenvironment Factors In Tissue Samples of Iraqi Women With Breast Cancer

المستخلص: Breast cancer (BC) is the most common cause of cancer - related deaths in women. It continues to rank as one of the top killer of women. This cancer increased in frequency in the last years in Iraq. The aim of this study was to shed light on the immunohistochemical for some factors that could be affect on development of microenvironment in breast cancer of Iraqi patients. And these factors include CD133 as a marker for breast cancer stem cells (BCSs), and also studying tumor necrosis factor alpha (TNF - ?) and transforming growth factor beta (TGF - ?). 53 samples Formalin - fixed, paraffin - embedded (FFPE) wax block appeared their ages were range from 29 - 70 year with a mean age of 48.45 years. 32 patients with breast carcinoma and 21 patients with benign breast lesions were included in this study for comparison. The results of this study show that the CD133 positive expression was found in (56.2%) of Iraqi breast cancer cases. Also the result of this study show that (62.5%) positive expression from both (TNF - ? &TGF - ?) of breast cancer cases, compared with sample of benign breast lesion. The results show (52.3%) positive expression of TNF - ? and (28.57%) positive expression of TGF - ? of samples with benign breast lesion, there is a significant different between studied samples, compared with (19.05%) samples positive expression of CD133 of sample with benign breast lesion so there is a significant different between the samples with breast cancer and benign breast lesion. Also the results show there is a positive relationship correlation between (TGF - ?) expression and (TNF - ?) expression, while there were no relationship correlation between (TGF - ?) and CD133 and no relationship correlation between TNF - ? and CD133. The results show there is a positive correlation between the grade and breast cancer with the three different expressions of marker but in different strongest correlations between (TNF - ?) and (TGF - ?) with the graded but this correlation becomes weak with CD133 marker the value of significant. According to the relationship in breast cancer case in this study between the studied markers and stage of case it shows (TNF - ?) has a strong positive correlation while the correlation appear week between the stage of this studies case and end each of TGF - ? and CD133. For this we concluded from the results there high expression of CD133 and TNF - ? indicators and TGF - ?. CD133 could use in diagnosis of the cancer cell and the high expression of TNF - ? & TGF - ? indicate that these factor play important roles in tumor microenvironment metastasis. And the strong correlation between the expression of these markers with grade and stage of breast cancer
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: اخلاص مشرف عيدان
اسم المؤلف: فاطمة سمير عبد الرزاق الزبيدي

دراسة بعض الجوانب المناعية والبكتريولوجية لمرضى ذات الرئة Pneumonia Study Some Immunological And Bacteriological Aspects of Pneumonia Patients

المستخلص: جمعت 120 عينة قشع من اشخاص مصابين بمرض ذات الرئه للمدة من ايار 2013 الى شباط 2014 من ثلاثة مستشفيات في مدينة بغداد وشملت مستشفى اليرموك التعليمي, مستشفى دار التمريض الخاص, مستشفى بغداد التعليمي. شخصت العينات باستخدام الفحوص البايوكيميائية, نظامApi 20 E و| From May 2013 to Feburey 2014, 120 sputum and Aspiration samples of patients with pneumonia disease were collected from different hospitals in Baghdad included : Al Yarmouk Teaching Hospital, Hospital Nursing Home sector and Baghdad Teaching Hospital. All samples were diagnosed by biochemical tests, Api 20 E and Api20 strep. Systems, The results was found to be 28 isolates (23.3%) belong to K.pneumoniae, 26 isolates (21.7 %) belong to S.pneumonia while 66 isolates(55%) belong to causes : E.coli, Pseudomonas sp., Moraxella catrrhalis, S.pyogens, Monilia and S.aureus. From (55) blood samples of pneumonia patients and 30 blood samples from healthy individuals, number of white blood cell (Neutrophil) counts were measured, percentage of Neutrophil cell count in female (53%) higher than the cell count in male(47%). Some markers of pneumonia disease were studied such as ratio of infection between male and female, chronic and acute cases and smoker and non smoker, the results were showed the ratio of infection in female more than in male, (60%, 40%), respectively. And number of acute cases of pneumonia patients 41(74.55%) more than chronic cases 14(25.45%). While infection ratio in smoker patients more than nonsmoker patients at ratio 24(57.14%)18(42.86%), respectively. The result of interleukin - 8 level through acute and chronic phases in pneumonia patients sera was showed high level in patients compared with healthy persons (86.4403 ± 25.50919 vs. 58.7636 ± 4.73152 pg / ml, respectively) with a non significant difference statistically (P?0.05). Also in the age group (age 15 - 60years), The results showed the results interleukin 8 levels higher than the age groups of (2month - 3years) and (age 60 - 85) (93.63 ± 51.65, 68.88 ± 17.17, 65.68 ± 11.73 pg / ml, respectively) with non significant difference (P?0.05). The result of Leukotriene B4 level through acute and chronic phases in pneumonia patients sera showed high level in patients compared with healthy persons (36.00 ± 3, 82 vs. 25.96 ± 4.44 pg / ml, respectively) with a significant difference (P?0.05). Also in the age group (two months - 3 years) were showed the results interleukin 8 levels higher than the age groups (age 15 - 60) (age (60 - 85)(33.61 ± 11.06, 19.29 ± 4.67, 16.86 ± 4.51 pg / ml respectively) with no siginificant difference (P?0.05), the correlation coeifficient between interleukin - 8 and leukotriene B4 was (0.12) with no siginificant difference. The phagocytic activity was determined in pneumonia patients sera according to measurement C3 protein level through acute and chronic phases, the result were showed the ratio of C3 protein levels in healthy persons more than in patients (135.93 ± 12.74, 133.19 ± 12.01 pg / ml, respectively) with a difference was not significant statistically (P?0.05).
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: رسمية عبد ابو ريشة
اسم المؤلف: كرم رياض حسن الجراح

التنميط الوراثي لمستضدات التطابق النسيجي في عينة عراقية من مرضى السكري النوع الثاني Hla Genotyping In A Sample of Iraqi Type 2 Diabetes Mellitus Patients

المستخلص: داء السكري مرض واسع الانتشارعالميا تختلف نسبة الاصابة بين البلدان المتطورة والنامية ويعتبر المسبب الرئيسي للاعاقة والموت في العالم.داء السكري النوع الثاني هو الاكثر حدوثا حيث تبلغ نسبة الاصابة (90 - 95%) من مجموع المصابين بالسكري للانواع الثلاثة الرئيسية. | Sixty of non insulin dependent diabetes mellitus (NIDDM) patients who were diagnosed according to American Diabetes Association criteria (ADA) 2007 were selected from the specialized center of endocrinology and diabetes (Baghdad AL - Russafa Health Directorate) during the period between first of May 2013 to last of October 2013.Their age ranged 35 - 70 years. Fourty apparently healthy individuals their age ranged (35 - 70) years were considered as control. Enzymatic colorimetric methods used for measured FBS (fasting blood sugar) and HbA1c (glycohemoglobin) and enzyme linked immunosorbent assay (ELISA) for hormones and enzyme markers. Fasting blood sugar revealed high significant in patients with median (11.6 mmol/L vs. 5.9 mmol/L) and (P<0.001) in comparison to control groups.Elevation of glycated hemoglobin (HbA1c) with mean (9% vs. 5.5%) in comparison to control groups. Another reliable marker are the concentration Adpionectine hormone, Insulin hormone and adenosine deaminase activity the results of those estimated significantly difference between levels mean (20.7 vs. 34 ng /ml) in patients compared to healthy subjects (P<0.001) for adiponectin ; mean (106.6 vs. 59.3 ng/ml) for ADA (adenosine deaminase) with (P<0.001) and the median (12.1 vs. 16 uIU/ml) for insulin hormone with (P 0.001). In order to investigate the accuracy of previously mentioned tests, a statistical analysis [Receiver - Operator Characteristics (ROC)] has been applied to show the accuracy, specificity and sensitivity of the tests under test.This analysis revealed that serum ADA activity is the best marker with highly specificity 100%, sensitivity 100%, and accuracy 100% while; FBS was the best test with highly specificity 100%, sensitivity 100% and 100% accuracy in comparison with other tests. It was denoted that type2 DM was associated with certain HLA class II alleles were analyzed for their genotyping by Polymerase Chain Reaction - Sequences Specific Olegionucleotide (PCR - SSO) technique. The present study revealed that diabetic patients were positively associated with HLA - DQB1*0201 (83% vs. 5.0%) which is the most prevalent in patients followed by DRB1*1137 (46.7% vs. 0.0%); DRB1*0401 (41.7% vs. 2.5%), and DRB1*1306(15% vs.0.0%) while HLA, A*0201;B*3559;Cw*0410 and DQB1*0501 is negatively associated in type 2DM in comparison with healthy control groups.This study has shown that there is no significant association between FBS, HbA1c, serum insulin, HOMA2(Homeostatic Model Assessment2) ? - function, HOMA2 - IR, serum adiponectin, serum ADA and HLA alleles(DQB1*0201, DRB1*1137, DRB1*0401, DQB1*0501, DRB1*1306) in spite the significant associated between FBS and serum ADA and HLA - DRB1*0701 allele with (P 0.021, P 0.008) respectively.The current result concluded that there may be an important role for HLA genotyping in arising the chance for enhancing the susceptibility for either disease development or protection against its initiation.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الدرجة: دكتوراه
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: محمد ابراهيم نادر بتول حسن الغرابي
اسم المؤلف: احمد كاظم محمد

انشاء وتوصيف لخط سرطان الثدي الخلوي العراقي Establishment And Characterization of Iraqi Breast Cancer Cell Line

المستخلص: Several primary cultures were initiated from different samples obtained from Iraqi female patients of breast tumor, one sample was successful, and this sample was histological diagnosed as breast cancer infiltrating ductal carcinoma.The cell suspension was cultured in tissue culture flask and confluent monolayer achieved after 16 days from primary culture. The continuous subculture was done in grown cells in tissue culture flask each 48 - 96 hrs. Between subculture to other until across 50 passages through11months.In our current study different experiments were done to characterize the cultured continuous cells, which are studying the growth curve of the new established cell line and calculating the population doubling time that have been 22 hours.Furthermore, a morphological study was carried out by staining the cells with hematoxilin and eosin dyes. The cells were elongated multi - polar epithelial like cells with nuclear polymorphism and multi - nuclei, in addition to high nuclear to cytoplasm ratio, all these characters of the malignant tumor cells.The Cytogenetic study showed chromosomal aberrations with many numerical changes among the tumor cells and abnormal structure gives chromosomes with unknown origin called marker chromosome. In furthermore the G - band stained of normal 46XX chromosome was done to facilities the comparisons between chromosomes of the new established breast cancer cell line and normal chromosomes aberrationsImmunocytochemistry examination was done for the tumor cells grown in multi well tissue culture slide chamber to detect the presence of some hormonal receptors (ER and PR) gives negative result, and to test Her - neu2 gene that gives week positive result.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: الانكليزية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: محفوظة عباس عمران احمد مجيد الشمري
اسم المؤلف: مرتضى عادل الشامي