عرض: 25 50 75 100 النتائج

نتائج البحث: 25 من أصل 24,037

تشخيص سلالات فايروس تقزم واصفرار الشعير Barley yellow dwarf virus (BYDV) في العراق وتحديد جينات المقاومة في تراكيب وراثية للشعير ومقاومة المرض Diagnosis The Strains of Barley Yellow Dwarf Virus (BYDV) In Iraq And Determine The Resistance Genes In The Genotypes of Barley And Disease Control

اسم المؤلف: عبد القادر خضير عباس
اسم المشرف: ميسر مجيد جرجيس
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - الامراض
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: شخص فايروس تقزم واصفرار الشعير (BYDV) Barley yellow dwarf virus وسلالاته PAV، MAV وRPV باعتماد اختبار اليزا المصلي ELISA وباستخدام مصول مضادة متعددة النسيلة ووحيدة النسيلة. اشارت نتائج الكشف عن فايروس BYDV وسلالاته الى انتشار الفايروس في اكثر حقول زراعة ا | Barley yellow dwarf virus (BYDV) and it's strain PAV, MAV and RPV have been diagnosed using polyclonal and monoclonal antibodies. The results showed the spread of BYDV in most of barley fields in Iraq. Nineveh governorate recorded the highest infection ra

التزامن ورنين التشاكه والعشوائي في الاتصالات البصرية العشوائية Synchronization, Coherence And Stochastic Resonance In Chaotic Optical Communications

اسم المؤلف: عيدان عاصي عبد الله الزبيدي
اسم المشرف: قيس عبد الستار النعيمي
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: في هذا العمل، درسنا ثلاث ظواهر ناجمة عن الضوضاء، تم تطبيقها على نظام شواش، ليزر شبه والتزامن )CR) رنين التشاكه، (SR) موصل بتغذية الكتروبصرية عكسية هي : الرنين العشوائي التام، بالرغم من ان تطبيقاتها لازالت واعدة نسبيا الا انه ليس هناك شك في ان تصبح مستقبلا | In this work, we studied three noise - induced phenomena, applied to a chaotic semiconductor laser system with optoelectronics feedback regime : the stochastic resonance (SR), coherence resonance (CR) and noise - induced complete synchronization, while th

تحضير ودراسة بعض الخصائص الفيزياوية والميكانيكية لمتراكبات الايبوكسي الدقائقية الهجينة The Preparation And Studying of Some Physical And Mechanical Properties For Hybrid Particular Composites Epoxy

اسم المؤلف: سعاد حامد العيبي الربيعي
اسم المشرف: عبد الحميد رحيم الصراف بلقيس محمد ضياء الدباغ
الموضوع العام: علوم الفيزياء
السنة: 2008
الموضوع الدقيق: الفيزياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة باستعمال راتنج الايبوكسي (Epoxy) نوع Thortex بوصفها مادة اساس للمواد المتراكبة وتم استعمال راتنج الفينول - فورمالديهايد نوع نوفولاك كمادة مطعمة ومسحــوق الزجاج (Glass - Powder) ومسحوق الالمنيوم (Al - Powder) بوصفها مواد مدعمة وبكسر حجم | This study was performed by using the Epoxy resin type Thortex as matrix to the composite materials with the phenol - formaldehyde type Novolac and with glass powder and the Alum. Powder were used as reinforced material of volume fraction amounting to 40%

تحضير غشاء نانوي من مادة اوكسيد الخارصين بطريقتين واستخدامه في تطبيقات الخلايا الشمسية Preparing of ZnO Nano Thin Film By Two Methods And Its Use in Solar Cells Applications

اسم المؤلف: رافع عبد الله منيف حلاب الجبوري
اسم المشرف: صبري جاسم محمد الحر رائد عبد الوهاب اسماعيل
الموضوع العام: علوم الفيزياء
السنة: 2012
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تم تحضير اغشية اوكسيد الخارصين النانوية النقية (ZnO)والمشوبة بالمغنسيوم (ZnO : Mg) باستخدام تقنية الرش الكيميائي الحراري وتقنية ترسيب الحمام الكيميائي على ارضيات زجاجية وسليكونيه احادية البلورة نوع (n - type) وعند حرارة قاعدة K)648) للرش الحراري و(443K) ل | In this Research nanometer size ZnO films was prepared using pegrolesis chemical spray (SCP) and chemical bath deposition (CBD) techniques. Two types of substrates were used, glass and n - type silicon. The temperature of the substrate during deposition w

انموذج رياضي لحساب خصائص الانتشار في الالياف البلورية الفوتونية Mathematical Model for Calculate Propagation Properties in Photonic Crystal Fibers

اسم المؤلف: مشعان عطا الله محمود الهيتي
اسم المشرف: لطفي علي محمود عبد الغفور ابراهيم عبد الله
الموضوع العام: علوم الفيزياء
السنة: 2012
الموضوع الدقيق: علوم الفيزياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: على الرغم من الدور المهم الذي لعبته الالياف الضوئية التقليدية في مجال الاتصالات، نهاية القرن العشرين، الا ان نوافذ الاتصالات المحدودة دفعت الباحثين للبحث عن الياف ذات تصاميم يمكن التحكم في مواصفاتها والحصول على نوافذ اتصالات جديدة تتلائم ومتطلبات العص | In spite of the important role of the photonic crystal fibers played in the field of communication at the end of 20th century, the limited ability of these fibers pushed the investigators to design new ones that fiber specifications can be pre - controlle

قياس معاملات توهين الاشعة السينية للعناصر ذات الاعداد الذرية (Z ? 30) واكاسيدها X - Ray Attenuation Coefficients Measurement of Elements With (Z ? 30) Atomic Numbers And Their Oxides

اسم المؤلف: محسن حسن علي الخفاجي
اسم المشرف: رائد نجيب رزوقي فريد مجيد محمد
الموضوع العام: علوم الفيزياء
السنة: 2012
الموضوع الدقيق: علوم الفيزياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تم في هذه الدراسة تحضير نماذج من الالمنيوم والتيتانيوم والنحاس والخارصين بسماك مختلفة، اكسدت جميع النماذج عند درجة الحرارة (500, 400, 300, 200, 100) ?م في فترات (5 - 0.5) ساعة. واجريت قياسات التوهين للاشعة السينية باستخدام وحدة الاشعة السينية بانبوبة تش | In this study we prepared samples from aluminum, titanium, copper and zinc with different thickness. All samples were oxidized at temperatures (100, 200, 300, 400, 500)°C at oxidation times of (0.5 - 5) hours. Characteristic X - ray was used to determine

تحضير ودراسة خصائص كاشف المفرق الهجين نوع Nano CdO / Si نانوية التركيب بطريقة ترسيب الحمام الكيميائي Preparation And Characterization of Nanostructured Cdo / Si Heterogunction Detector By Using Cbd Method

اسم المؤلف: هاني هادي احمد الحديثي
اسم المشرف: عبد المجيد عيادة ابراهيم السامرائي صبري جاسم محمد رائد عبد الوهاب اسماعيل
الموضوع العام: علوم الفيزياء
السنة: 2012
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: في هذا البحث، تم ولاول مرة تصنيع كاشف المفرق الهجين نوع CdO/Si وذلك بترسيب تراكيب نانوية(Nanostrcture) على هيئة اغشية رقيقة من مادة اوكسيد الكادميوم CdO بطريقة الترسيب بالحمام الكيميائي (CBD)على شرائح زجاجية وسليكونية احادية البلورة نوع (p - type) والتي ت | This work is an attempt to fabricate for the first time of CdO/Si heterojunction detector were by depositing nanostructure of CdO thin film on p - type single crystal silicon wafers and glass substrates by chemical bath deposition technique, which is a si

تصنيع ودارسة خصائص كاشف ضوئي من المفرق الهجيني cdo : Al/si Fabrication And Study of Photodetector Properties From Cdo : Al/Si Heterogunction

اسم المؤلف: اياد احمد صالح
اسم المشرف: سمير عطا مكي علية عبد المحسن شهاب
الموضوع العام: علوم الفيزياء
السنة: 2015
الموضوع الدقيق: الفيزياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: في هذا البحث, تم تصنيع كاشف ضوئي من المفرق الهجين (n - CdO : Al/p - Si) ثم دراسة خصائصه, والمحضر من ترسيب اغشية (CdO) النقية والمطعمة بالالمنيوم (CdO : Al) بطريقة التبخير الحراري في الفراغ على قواعد سليكونية احادية البلورة باتجاهية (111). اذ تم تحضير هذه | In this work, photovoltaic detector of (n - CdO : Al/p - Si) heterojunction has been fabricated and its properties were studied, which prepared by deposition CdO and CdO : Al via thermal evaporation under vacuum method on an (111) oriented single crystal

تصنيع مفرق هجين للمركب (CuIn1 - XGaxSe2) واستخدامه كخلية شمسية Fabrication Heterojunction of Compound (Cuin1 - Xgaxse2) And Used As Solar Cell

اسم المؤلف: علي حسين عبد الرزاق
اسم المشرف: ايمان حميد خضير حسون الميالي بشرى كاظم
الموضوع العام: علوم الفيزياء
السنة: 2015
الموضوع الدقيق: الفيزياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: حضرت في هذا البحث سبائك للمركب الرباعي (CIGS) على وفق الصيغة الكيميائية (CuIn1 - xGaxSe2) لقيم مختلفة لـ (x)، (x= 0.3, 0.6, 0.9) باذابة عناصر السبيكة في انبوب مفرغ من الكوارتز تحت ضغط (2×10 - 3mbar)، وباجراء فحوصات حيود الاشعة السينية لمساحيق السبائك الث | In this research (CIGS) quarter compound alloys prepared for chemical formula (CuIn1 - xGaxSe2) for different x (x=0.3,0.6,0.9) by melting them in a evacuated quartz tube (2×10 - 3 mbar).XRD analysis show that all three alloy prepared (powder) are polycr

دراسة النشاط الاشعاعي لنماذج من تربة مدينة الحيدرية في محافظة النجف الاشرف The Study of Radioactivity In Soil Samples From Al - Haidariya City In Al - Najaf Al - Ashraf Governorate

اسم المؤلف: سجاد علي امين
اسم المشرف: علي خلف حسن السنيد
الموضوع العام: علوم الفيزياء
السنة: 2014
الموضوع الدقيق: الفيزياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: لدراسة النشاط الاشعاعي لتربة مدينة الحيدرية في محافظة النجف الاشرف اختير (30) موقعا لاخذ العينات من المدينة وجمع من كل موقع اربع عينات الاولى من التربة السطحية (0 - 10)cm والثانية بعمق(10 - 20)cm والثالثة بعمق (20 - 30)cm والرابعة بعمق (30 - 40)cm ليكون | To study the radioactivity of soil of AL - Haidariya city in AL - Najaf AL - Ashraf, (30) locations had been selected to take samples from this city, four samples were collected from each site, the first from surface soil (0 - 10) cm, the second depth (10

تحضير وتشخيص بعض مشتقات الملئيمات الجديدة الحاوية على حلقات 2 - بايرازولين وبايرمدين - 2 - اون / ثايون ودراسة فعاليتها البايولوجية ثم بلمرتها ذايا او مع بعض معوضات الفاينيل Preparation And Identification Some New Maleimide Derivatives Containing 2 - Pyrazoline And Pyrimidin - 2 - One / Thione Rings And Study of Biological Activity Then Homopolymerization Or With Some Substituted Vinyl

اسم المؤلف: غزوان حسن عبد الوهاب الصميدعي
اسم المشرف: احمد شهاب حمد الجنابي خالد مطني محمد الجنابي
الموضوع العام: علوم الكيمياء
السنة: 2008
الموضوع الدقيق: علوم الكيمياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: ترتبط بعض الامراض النفسية مثل مرض الاكتئاب الشديد والفصام بتغيرات مناعية حتى ولو كان الشخص سليم من الناحية الجسمية. على الرغم من ان هذين المرضين لايعتبران حاليا من الامراض المناعية الاساسية لكن توجد ادلة تقترح كون الالتهاب يلعب دورا في عملية الاصابة بالاك | Psychiatric illnesses, particularly major depressive disorder (MDD) and schizophrenia, are associated with inflammatory processes even in individuals who are otherwise physically healthy. While it is unlikely that MDD and schizophrenia are a primary inflammatory disorder, evidence suggesting that inflammation plays a subtle role in the pathophysiology of MDD and schizophrenia has been obtained. Several studies have shown that proinflammatory markers have increased in these disorders. However, these studies have included patients with obvious inflammation, i.e., patients with C - reactive protein >6 mg/L. A comprehensive study concluding that these conditions are inflammatory disorders and inflammation is due only to these two disorders have not been performed. In the present study, the inflammatory natures of MDD and schizophrenia were investigated. To achieve this goal, serum levels of the interleukins - 6 (IL - 6) and interleukins - 18 (IL - 18), tumor necrosis factor alpha (TNF?), and soluble interleukin - 2 receptor (sIL - 2R) in MDD and schizophrenia were obtained and compared with those of the control group. The total serum and ionized levels of calcium (Ca) and magnesium (Mg) in the studied groups were then measured.Significant increases (p < 0.05) in the serum levels of IL - 6, IL - 18, TNF?, and sIL - 2R in the MDD and schizophrenic groups were observed in comparison with those of the control group. In general, the schizophrenic group showed higher levels of inflammatory markers than the MDD and control groups. These results indicate a clear inflammatory state in both disorders. The schizophrenic patients exhibited significant changes in the ratios of T. Ca/Mg and I. Ca/Mg in comparison with those of the healthy and MDD patients; this finding reveals that T. Ca/Mg and I. Ca/Mg ratios are better indicators of cation state than serum cation levels alone.Basing on the results of the present study, we concluded that the immunological responses of the MDD and schizophrenia are significantly stimulated. These conditions may be considered inflammatory disorders because of increased pro - and inflammatory factors in both disorders.Anti - inflammatory drugs may be potentially used as an adjuvant aid in treating schizophrenia and MDD. Future studies may involve investigating other proinflammatory markers and following up results of treated patients that utilized and did not utilize anti - inflammatory drugs.

تحضير ودراسة طيفية ومعالجة نظرية لمعقدات مزيج ليكاندي مشتقات البنزاميدازول والفينانثرولين

اسم المؤلف: دعاء حبيب محل
اسم المشرف: محاسن فيصل الياس
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء العضوية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الكلمات الدلالية:
  • تحضیر
  • حلقیة غیر متجانسة
  • جالكون، بایرازولین
  • ثنائي هایدروبایرمدین - - 2 اون
  • ثنائي هایدروبایرمدین - 2 - ثایون، بولي ایماید
  • فعالیة بایولوجیة
  • موجات فوق صوتیة Synthesis
  • Heterocyclic
  • Chalcone
  • derivatives
  • Pyrazoline
  • Dihydropyrimidin - 2 - one
الصفحات الاولى:
المستخلص: In this study, two new aromatic chalcones have been synthesized. The first one contain one amino phenyl group while the second contain two amino phenyl groups , from the reaction of 2 - acetothiophene or pamino acetophenone with p - or m - amino benzaldehyde respectively by Claisen - Schmidt condensation in alcoholic base medium (10%NaOH ).Also, some of heterocyclic compounds have been derived from these prepared chalcones as a derivatives for 2 - pyrazoline ring and for dihydro pyrimidine - 2 - one/thione as follows : 1 - Preparation of 3,5 - di (amino phenyl ) - 2 - pyrazoline (3,9) derivatives from condensation of (1,2) chalcone with hydrazine hydrate in absolute ethanol.2 - Preparation of 3,5 - di (amino phenyl) - 1 - phenyl - 2 - pyrazoline (4,10) derivatives by the reaction of chalcone (1,2)with phenyl hydrazine in presence of acetic acid.3 - Preparation of 3,5 - di (amino phenyl) - 1 - substituted - 2 - pyrazoline (5 - 7,11 - 13) from the reaction of chalcones (1,2) with semicarbazide, thiosemicarbazide and isoniazide in strong basic medium (45% NaOH) in absolute ethanol.4 - Preparation of 4,6 - di(amino phenyl ) - 3,4 - dihydro pyrimidine - 2 - one / thion (14,15) : from the reaction of chalcone(2)with urea and thiourea in presence of sodium ethoxide in absolute ethanol in two ways : thermal catalyse and ultrasonic waves with high yield for the last one.The phenyl amines derivatives (3 - 7,9 - 15)included benzidine (8) were converted into N - substituted maleimic acid (16 - 28) , by reacted it with maleic acid anhydride in ether or dry acetone.These derivatives (16 - 28) were undergo dehydration by using anhydrous acetic and sodium acetate to give N - substituted malimide products ( 29 - 41) in good yield.Also, the homopolymers (42 - 54) for these malimides were prepared by free radical polymerization with benzoyl peroxide as initiator and dimethyl formamide (DMF) as a solvent at 130 0C. PDF created with pdfFactory Pro trial version www.pdffactory.com The copolymers (55 - 104)have been prepared by the samemethod and initiator with vinyl acetate for polymers (55 - 67)and with acryl amide for polymers (68 - 72) and with allyl benzene for polymers (73 - 80) and with maleimide (31) for the polymers (81 - 88) , also with maleimide (32) for the polymers (89 - 96) and finally with maleimide (33) for the polymers (97 - 104).The structures of these new compounds were characterized by UV spectra (as a comparable study for unpolymered molecules ),IR (infra - red ) spectra and 1H - NMR (nuclear magnetic resonance ) spectra for some of the prepared compounds and followed by TLC (thin layer chromatography ).The stability of these compounds (9 - 12) were studied spectrally from the changing in its high absorbance values (peaks) ?max for their solutions at concentrations 2×10 - 5M in light and dark with time for comparability. These compounds showed good stability whichcan be suggested to be used as an organic reagents for analytical study especially with drugs and phenolic compounds.More ever, the identification study , here we have studied the heoretical study of formation heat for intermediates and final compounds bodies length of the bond which was agreement with the particularly results.This study ,also includes the biological activity for some of the prepared compounds against four kinds of germs which known by its resistance against antibiotics ,these are Staphylococcus aureus (positive for Gram stain )and Escherichia coli ,Pseudomonas aeruginosa and Salmonalla typhi (negative for Gram stain ) and studiesy the inhibition values for these compounds comparable with three of standard antibiotics as controls (ampicilline : C1, Cloxacilin : C2, erythromycin : C3) , and the results show the ability of these prepared compounds to inhibit all the used germs except Pseudomonas which shows clear resistant for less polarity comparable with control samples.

تحضير، تشخيص وتقييم بايولوجي لبعض الادوية المصاحبة المشتقة من بعض مضادات الالتهابات غير الستيرويدية Synthesis , Characterization And Biological Evaluation of Some Prodrugs Derived From Non - Steroidal Anti - Inflammatory Drugs (NSAIDs)

اسم المؤلف: مصطفى راجي عايد حمد
اسم المشرف: خالد مطني محمد الجنابي اياد سعدي حميد الدليمي
الموضوع العام: علوم الكيمياء
السنة: 2012
الموضوع الدقيق: الكيمياء الحياتية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: اجري هذا البحث لايجاد العلاقة بين العجز الكلوي المزمن ومستويات بعض المتغيرات الكيموحيوية عن طريق قياس مستوى انزيم الرينيين والفيوكوز الكلي (TF), الفيوكوز المرتبط بالبروتين (PBF) , البروتين المرتبطة بالسكريات السداسية (PBHex) وعدد من المتغيرات الكيموحيوية | This study has been done to determine the relationship between chronic renal failure and the level of some biochemical parameters (Rennin enzyme, Total Fucose(TF), Protein bound Fucose(PBF), protein bound Hexose (PBHex), and some other biochemical parameters including glucose , Urea, createnine, Uric acid, High density lipoproteins Cholestrol(HDL - c.), low density lipoproteins Cholestrol (LDL - c.) and electrolytes (Na+, K+ and Ca+2). Case study included (80) patients with chronic renal failure,Who admitted to the Kirkuk General Hospital Department of Dialysis , there ages were (31 - 60 years) of both sexes , patients has been divided into subgroups according to age {(31 - 35),(36 - 40),(41 - 45),(46 - 50),(51 - 550),(56 - 60)}year and severity compared with (45) normal persons as control with same ages, the results was as follows : Results showed a significant increase (P?0.01) in the level of rennin enzyme (5.013±0.052 ng/mL.h) of patients before dialysis compared with control group (2.58±0.32 ng/mL.h) for different age groups, and there was a significant increase slightly for different age groups.There was a significant increase (P?0.01) in the level of TF and PBF (12.14±0.89 mg/dL),(7.450±0.18 mg/dL) respectively compared with control group (28.76±0.03 mg/dL), (2.26±0.27 mg/dL) respectively for different age groups, and there was no significant correlation between age groups or sex.There was significant decrease (P?0.01) in the level of protein bound hexose PBHex, (123.35±0.9 mg/dL) compared with control group (89.76±0.9 mg/dl) for different age groups, and there was no significant correlation between age groups or sex.There was a significant increase (P?0.01) in the level of glucose (5.072±0.13 mmol/L) compared with control group (7.03±0.46 mmol/L) for different age groups, and there was a significant increase for females (7.40±0.7 mmol/L) compared with males (6.84±0.4 mmol/L).There was a significant increase (P?0.01) in the level of urea, uric acid and createnine, (5.66±0.24 mmol/L) , (336.5±1.4 mmol/L) , (90.8±1.3 mmol/L) respectively when compared with control group (34.27±0.9mmol/L) ,(516±1.8 mmol/L), (866±1.9 mmol/L) respectively for different age groups, and there was significant increase for males compared with females.There was a significant increase (P?0.01) in K+ level (5.896±0.05mmol/L) compared with control group (4.253±0.12mmol/L) and there was no significant correlation for males compared with females.There was a significant decrease (P?0.01) in Na+, Ca+2 level, (2.317±0.044 mmol/L), (144.35±1.6mmol/L) respectively compared with control group (1.89±0.046 mmol/L) , (121.00±1.1mmol/L) respectively and there was no significant correlation for different age groups or males and females, while there was a a significant correlation for male (1.979±1.1mmol/L) compared with female (1.768±0.073mmol/L).Result showed a significant decrease in HDL_c in the serum of patients before dialysis (1.263±0.027 mmol/L) compared with control (0.701±0.014 mmol/L) and there was a significant increase in HDL - c for female group compared with male group.There was significant increase in LDL_c in the serum of patients before dialysis (2.946±0.019mmol/L) compared with control (5.1±0.68mmol/L) and there was a significant increase in LDL - c for female (6.30±1.3 mmol/L) compared with male group(5.076±0.023mmol/L).

تحضير وتشخيص بعض مشتقات السكارين العضوية ومعقدات السكارين ومشتقاته الفوسفينية مع بعض الفلزات Synthesis And Characterization Some of Organic Saccharin Derivatives And Metal Complexes of Saccharin And Its Phosphine Derrivative

اسم المؤلف: عفراء صابر شهاب الزوبعي
اسم المشرف: احمد عبد الحسن احمد الكاظمي صبحي عطية محمود الجبوري
الموضوع العام: علوم الكيمياء
السنة: 2012
الموضوع الدقيق: الكيمياء العضوية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمن البحث تحضير عدد من الادوية المصاحبة المشتقة من مضادات التهاب غير ستيرويدية تم الحصول عليها من معمل ادوية سامراء هي الايبوبروفين، الاندوميثاسين، النابروكسين، الاسبرين، الكابتوبريل والايزونوزايد. عملية تحوير ال | In this study, a number of prodrugs have been synthesized starting from Non - steroidal anti inflammatory drugs such as Ibuprofen, Indomethacin, Naproxen, Aspirin, Captopril and Isoniazid. The modification process started from the conversion of carboxyl group which is existing in these drugs (except in Isoniazid) into esters, chlorides and acid hydrazide.The drugs have been linked to each other and to itself via amide linkage through the reaction of their acid chlorides and acid hydrazides to afford 1,2 - diacyl hydrazine derivatives(M17 - M27). Some acid hydrazides have been treated with isophthaloyl chloride to Benzene - 1,3 - dicarbo hydrazide - 1,3 - Bis substituent(M28 - M30). The same hydrazides have been treated with Benzen - 1,3,5 - tri carbonyl tri chloride to afford Benzen - 1,3,5 - tricarbhydrazide - 1,3 - Tris substituent (M31 - M33). Compounds M28 and M29 have been treated with POCl3 to afford Bis - substituent - 5 - phenyl - 1,3,4 - Oxadizole(M34 - M35). Another modification onto drugs has been done through the linking of these drugs with quinazoline nucleus through amide linkage to afford N - (4 - oxo - 2 - phenylquinazolin - 3(4H) - yl)amide - substit derivatives (M38 - M42). Another modification has been carried out through the reaction of hydrazides with Aromatic aldehydes to afford the mono hydrazide - hydrazones derivatives or Schiff's bases (M43 - M65) and di hydrazide - hydrazones derivatives in which two moles from hydrazide have been treated with one mole of dialdehyde(M66 - M73). In addition, new hydrazones have been synthesized through the reaction of hydrazides with Isatin nucleus which is well known as biological active center to afford the compounds (M74 - M77) with good biological activity. Benzodiazepines have been obtained through the reaction of Isoniazid hydrazones with glycene phthalimide and p - aminobenzoic acid(M82 - M92). Indomethacin hydrazones have been treated with acetic anhydride to afford 1 - [2 - (substituent) - 5 - (5 - methoxy - 2 - methyl - 1H - indol - 3 - ylmethyl) - [1,3,4]oxadiazol - 3 - yl] - ethanone derivatives (93 - M97). The Derivatives of 5 - substit - 2 - Mercapto - 1,3,4 - Oxadiazole(M98 - M103) have been obtained through the reaction of the drug hydrazides with CS2 in the presence of KOH. The oxadizoles have been treated with hydrazine hydrate to afford 4 - amino - 5 - sub - 4H - 1,2,4 - triazole - 3 - thiol derivatives for the Non - steroidal anti - inflammatory drugs(M104 - M107). In addition, 5 - sub - 1,3,4 - Oxadiazole - 2 - amine derivatives have been obtained through the reaction of drug hydrazides with BrCN(M108 - M111). The compound 5 - {1 - [4 - (2 - methylpropyl)phenyl]ethyl} - N3 - phenyl - 4H - 1,2,4 - triazole - 3,4 - diamine (M113) has been synthesized through the reaction of the compound 2 - {2 - [4 - (2 - methylpropyl)phenyl]propanoyl} - N - phenylhydrazinecarbothioamide (M112) with hydrazine hydrate. The ester compound 2 - hydroxyphenyl 2 - [4 - (2 - methylpropyl)phenyl]propanoate (M114) have been obtained through the reaction of Ibuprofen chloride with salicylic acid. The two compounds, 2 - (4 - Isobutyl - phenyl) - propionic acid [1 - (4 - methyl - piperazin - 1 - ylmethyl) - 2 - oxo - 1,2 - dihydro - indol - 3 - ylidene] - hydrazide (M115) and 2 - (4 - Isobutyl - phenyl) - propionic acid [5 - fluoro - 1 - (4 - methyl - piperazin - 1 - ylmethyl) - 2 - oxo - 1,2 - dihydro - indol - 3 - ylidene] - hydrazide (M116) have been obtained through the reaction of M74, M77 with excess of formaldehyde and equimolar of N - methyl piperazine.The synthesized compounds have been identified using Infra - red spectroscopy FTIR, Nuclear Magnetic resonance (1H,13C), Mass spectroscopy and X - Ray. In addition , the changes in physical properties such as melting points, boiling points and color have been considered. Finally, the biological activity of some synthesized compounds has been evaluated and some of them have shown good biological activity especially those containing Isatin nucleus (M74 - M77) and (M115 - M116).

تحضيروتشخيص بعض المشتقات الجديدة لثنائي هيدروبريميدينون باستخدام تفاعل بكينيلي ودراسة فعاليتها البايلوجية Synthesis And Characterization And Biological Activity Study of Some New Dihydropyrimidinones Derivatives Via Application Biginelli Reaction

اسم المؤلف: احمد ماهر فهيم
اسم المشرف: نبيل عبد عبد الرضا
الموضوع العام: علوم الكيمياء
السنة: 2014
الموضوع الدقيق: الكيمياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The present work involved synthesis of new indole derivatives containing other heterocyclic ring. These derivatives could be divided into the following parts : 1. The first part involved synthesis and characterization of novel 1,3 - diazidine[XIII]a - d and [XIV]a - d by the following steps : Scheme(1).a. Synthesis of 3 - [(5 - hydrazinyl - 4 - phenyl - 1,2,4 - triazol - 3 - yl)methyl] - 1H - indole [VI], this compound is synthesized by neucleophilic substitution reaction of 1,2,4 - triazole - 5 - thiol compound [V]a with excess of hydrazine hydrate in absolute ethanol as a solvent.b. Synthesis of 2 - {5 - [(1H - indol - 3 - yl) - propyl] - 4 - phenyl - 1,2,4 - triazol - 3 - yl} - thio - acetohydrazide[VIII]b, by the reaction of ester compounds[VII]b with excess of 80% hydrazine hydrate in ethanol under reflux.c. Synthesis of new Schiff bases[IX]a - d and [X]a - d by condensation reaction of primary amino compounds[VI] or [VIII]b with different aromatic aldehyde in absolute ethanol as a solvent.d. Synthesis of new N - acyl derivatives[XI]a - d and [XII]a - d by reaction of acetyl chloride with the synthesized Schiff bases[IX]a - d, [X] a - d in dry benzene.e. Synthesis of 1, 3 - diazetine derivatives[XIII]a - d and [XIV]a - d by addition reaction of compound[XI]a - d or [XII]a - d with sodium azide in dimethylformamid as a solvent at (55 - 60)0C temperature.2 - While the second part involved synthesis and characterization of novel thiazolidine - 4 - one compounds[XXI]a - d and [XXII]a - d by the following steps : Scheme(2).a. Synthesis of 2 - [5 - (2 - methyl - 1H - indol - 3 - yl) - 4H - 1,2,4 - triazol - yl] - thio - ethyl hydrazide[XVIII] by the reaction of ester compound[XVII] with excess of 80% hydrazine hydrate in ethanol under reflux.b. Synthesis of 2 - {5 - [(1H - indol - 3 - yl) - methylene] - 4 - phenyl - 1,2,4 - triazol - 3 - yl} - IXthio - ethylhydrazide[XVIII], by the reaction of ester compounds[XVII] with excess of hydrazine hydrate in ethanol under reflux. Also synthesize new Schiff bases [XIX]a - d and [XX]a - d by condensation reaction of acid hydrazide compounds [VIII]a or [XVIII] with different aromatic aldehyde in absolute ethanol as a solvent.c. Synthesis of new thiazolidine - 4 - one derivatives, [XXI]a - d and [XXII]a - d by refluxing equimolar amounts from Schiff bases[XIX]a - d and [XX]a - d with excess of thioglycolic acid in dry benzene.3. The third part involved synthesis and characterization of new series of thiazolidine - 4 - one [XXVII]a - c and [XXVIII]a - c by many steps reaction as follows : Scheme(3).a. Synthesis of new Schiff [XXIII]a,b, [XXIV]a,b by condensation reaction of acid hydrazide [III]a,b with two types of aromatic aldehyde in absolute ethanol.b. Synthesis of another type from thiazolidine - 4 - one derivatives [XXV]a, b and [XXVI]a, b from the reaction of Schiff bases [XXIII]a,b, [XXIV] a,b with excess of thioglycolic acid in dry benzene. The alkoxy derivatives of compounds [XXVII] and [XXVIII]; 2 - (4 - N - alkoxyphenyl) - 3 - (2 - methyl - 1H - indol - 3 - yl)amido - 1,3 - thiazolidine - 4 - one[XXVII]a - c and 2 - (4 - N - alkoxyphenyl) - 3 - (1H - indol - 3 - yl) acetamido - 1,3 - thiazolidine - 4 - one [XXVIII]a - c were synthesized by the reaction of terminal OH group of compound[XXV]a or [XXVI]a with different n - alkyl bromide in alkaline media.4 - The fourth part involved synthesis and characterization of amide derivatives of 1,3,4 - thiadiazole[XXXI]a - d, [XXXII]a - d and [XXXIII]a - d by the following steps : Scheme(4)a. Preparation of 2 - amino - 5 - (2 - methyl - 1H - indol - 3 - yl) - 1,3,4 - thiadiazole[XXIX] by cyclization reaction of 2 - (2 - methyl - 1H - indole - 3 - carbonyl) hydrazine carbo - thio amide [XV] in conc. H2SO4 under reflux followed by neutralized with liquid ammonia. While 2 - amino - 5 - [(1H - indol - 3 - yl)methyl] - 1,3,4 - thiadiazole[XXX]aand 2 - amino - 5 - [(1H - indol - 3 - yl)propyl] - 1,3,4 - thiadiazole[XXX]b synthesized by cyclization reaction under reflux of indole - 3 - acetic acid or indole - 3 - butric acid with thiosemicarbazide in the presence of phosphorous oxychloride.b. Synthesis of new amide derived from 2 - amino - 1,3,4 - thiadiazole [XXXI]a - d, [XXXII]a - d and [XXXIII]a - d from the reaction equimolar of suitable 2 - amino - 1,3,4 - thiadiazoles[XXIX] and [XXX]a,b with different acid chlorides in DMF and pyridine as accepter.5. The fifth part involved synthesis and characterization of new hydrazones [XXXVII]a - d and [XXXVIII]a - d. These compounds were synthesized according to the follows steps : Scheme(5)a. Synthesis and characterization of 1 - (2 - methyl - 1H - indole - 3 - yl) carbonyl - 3 - methyl - pyrazol - 5(4H) - one[XXXIV] and 1 - [(1H - indol - 3 - yl) ethanoyl or butanoyl] - 3 - methyl - pyrazol - 5(4H) - one [XXXV]a, b by heating under reflux a solution of equimolar from acid hydrazides[III]a - c with ethylacetoacetate in absolute ethanol.b. Synthesis and characterization of 1 - [4 - acetyl - (1H - indol - 3 - yl)ethanoyl or butanoyl] - 3 - methyl - pyrazol - 5 - one [XXXVI]a, b through reaction of pyrazolone derivatives[XXXV]a,b with acetyl chloride in 1,4 - dioxane in presence of calcium hydroxide.c. Synthesis and characterization of novel hydrazones [XXXVII]a - d and [XXXVIII]a - d by the reaction of 4 - acetyl - pyrazolone compounds[XXXVI]a, b with phenyl hydrazine or substituted phenyl hydrazine in ethanol.The synthesized compounds have been characterized by their melting points, elemental analysis (C.H.N) and by their spectral data, FTIR, 1HNMR and Mass spectroscopy (of some of theme).Study of the biological activity of some of the synthesized compounds against Gram - positive bacteria (Staphylococcus aureus) and Gram - negative bacteria (Escherichia coli). The results showed that most of the tested

تخليق وتشخيص ودراسة الخصائص التفلورية والمضادة لفايروس نقص المناعة المكتسبة لبعض مشتقات البركنينولون الجديدة Synthesis, Characterization, Fluorescence Properties, Anti - Hiv Activity And Molecular Modeling Study of Some New Pregenolone Derivatives

اسم المؤلف: رواء علاوي كاظم القريشي
اسم المشرف: نجم عبود لعيبي المسعودي نبيل عبد عبد الرضا
الموضوع العام: علوم الكيمياء
السنة: 2015
الموضوع الدقيق: الكيمياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: نظرا لما تتمتع به مركبات الازو من تطبيقات مهمة في كل من المجالين الاكاديمي والتطبيقي فقد تمحورت دراستنا على تحضير ستة ليكاندات ازو جديدة مشتقة من المركب 5,4 - ثنائي فنيل اميدازول ودراسة السلوك التناسقي لكل منها مع ستة ايونات فلزية هي Co(II) , Ni(II) , C | Azo compounds have important applications in both of academic and applied fields, Accordingly, our study based on the synthesis of azo compounds derived from 4,5 - diphenyl imidazole and characterization of these azo compounds with their metal complexese containing Co(II) , Ni(II) , Cu(II) , Zn(II), Cd(II) and Hg(II) ions. Mass Spectra of ligands and their complexes were supported their molecular formula, while H1NMR spectra of ligands agreed well with the suggested structures , The C.H.N. and Atomic absorption analysis showed a good agreement with the calculated values , The purity of ligands and their complexes were examined by TLC and the values of Rf were values measured.Electronic spectra of complexes showed a bathochromic shift comparing to ligands spectra as a result of the coordination between the metal ions and ligands. The concentrations that obeyed Beer - Lambert Law of the complexes were determined from calibration curves , Mole Ratio Method indicate a ratio (1 : 2) (Metal : ligand) for the complexes of (DPEPI) and (DPDED) while other complexes with other ligands show a ratio (1 : 1) (Metal : ligand).Satiability Constants of the complexes were calculated and found that the Stability constants of complexes of the first ligand (DPEPI) were ( 2.955×10 12) >(2.294× 1011 ) <(1.121×10 12 ) < (1.108 ×1011 ) varied in the arrangement as Co(II)< Ni(II) < Cu(II)> Zn(II) which agree with Irving - Williams Series. This arrangement was applied on the complexes of the other ligands. The Conductivity measurements of complexes showed non ionic character. Apparently the magnetic susceptibility values agree with their values of the octahedral high spin complexes for Co(II)) while the complexes of Zn(II), Cd(II), and Hg(II) were diamagnetic. IR Spectra of complexes showed obvious changes of the frequencies of ? (N=N) of azo group and ?(C=N) of heterocyclic imidazole ring as result of coordination with metal ions, While the frequencies of ?(C=N) of azomethine group did not show any significant change for complexes comparing to the ligands spectra ,New bands were appeared in the region (400 - 500) cm - 1 in the spectra of complexes that due to frequencies of ?(M - N) because of coordination process. From the previous results the purposed structures of the complexes can be summarized as shown in the following : Proposal structure of (DPEPI) Complexes Proposal structure of (DIDHP), (DIDMP),

تحضير وتشخيص عدد من معقدات البلاديوم (??) والبلاتين (II) مع مزيج من ليكاندات الثايويوريا او الحلقات غير المتجانسة الحاوية على الامين مع السكارين او الثايوسكارين Synthesis And Characterization of Some Palladium (??) And Platinum(II) Mixed Ligand Complexes of Thiourea Or Heterocyclic Amine And Saccharin Or Thiosaccharine

اسم المؤلف: نور عبد المجيد ضياف الدوري
اسم المشرف: صبحي عطية محمود الجبوري مظهر يونس محمد المهيدي
الموضوع العام: علوم الكيمياء
السنة: 2012
الموضوع الدقيق: الكيمياء العضوية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تتناول هذه الاطروحة تحضير المركبات الحلقية غير المتجانسة ذات الحلقة الخماسية مثل معوضات الثايادايازول و3,2,1 - ترايازولين والثايازولدين - 4 - اون والحلقة الرباعية مثل الازتدين - 2 - اون والحلقة الثلاثية الاوكسازردين, فضلا عن تحضير الحلقة السداسية البرمدين | The thesis deals with hetero cyclic compounds with five member rings like thiadiazole substituted 1,2,3 - triazoline and thiazoldine - 4 - one, four member ring like azetidine - 2 - one and three member rings oxazirdine. Synthesis including six member ring compounds like pyrimidin - 2,4,6 - trione in addition to synthesis seven member ring compound like oxazepine are synthesized. In the present work 4 - bromo aniline is used as precursor for the synthesis of the required compound; the 2 - aminobenzothiazole - 6 - bromo (K1) is synthesized from the reaction of 4 - bromo aniline with potassium thiocyanate in presence of bromine, the later (K1) was reacted with ethyl chloroformate to give ethylcarbamate (K2),which is used for the synthesis of urea derivatives (K3 - 8).through (K2) reaction with substituted aniline. Pyrmidin - 2,4,6 - trione (K9 - 14) is synthesized from the reaction of malonic acid with urea derivatives in acetyl chloride.Schiff bases (K15 - 20) are prepared through the condensation of 2 - aminobenzothiazole with substituted benzyldehid. Seven member ring are synthesized by cyclaziation of Schiff bases (K15 - 20) by using malic anhydride in absolute ethanol to give 2 - aryl - 3 - N - 6 - bromobenzothiazole - 5 - 7 - dione oxazepene (K21 - 26). four member ring is prepared from the reaction of Schiff bases(K15 - 20) with chloro acetyl chloride in 1,4 - Dioxane in the presence of tri ethyl amine to give azetidine - 2 - one(K27 - 32). Thiazoldine - 4 - one(K33 - 38) is synthesized from the reaction of Schiff bases(K15 - 20) with thioglycolic acid in the presence of Zinc chloride. The Oxazirdine(K74 - 79) is also prepared from the reaction of Schiff bases(K15 - 20) with 3 - chloro per benzoic acid in pyridine. 2 - (2 - chloroacetamide) - 6 - bromobenzothiazole(K39) is prepared from the reaction of 2 - aminobenzothiazole - 6 - bromo (K1) with chloro acetyl chloride. The compound (K39) is used in the preparation of substituted aryl amino acetyl - 2 - amino - 6 - bromobenzothiazole (K40 - 46) through its reaction with substituted amine. The thiazoldine - 4 - one - 2 - imine (K47) is also synthesized from the reaction of compound (K39) with potassium thiocyanate. The thiazoldine - 4 - one - 2 - imine (K47) is reacted with substituted benzeldehyd to give 2 - (5 - (aryldin) - 2 - imino - 4 - oxo - thiazoldin - 3 - yl) 6 - bromobenzothiazole (K48 - 53).The 2 - (6 - bromobenzothiazole - 2 - ylimino) thiozoldine - 4 - one(K95) is prepared from the reaction of 2 - acetylamino - 6 - bromobenzothiazole(K39) with ammonium isothiocyanate in ethanol 96%. The thiazoldine - 4 - one(K95) is used in the preparation of 2 - (6 - bromobenzothiazole - 2 - ylimino) - 5 - (sub.Methyl) thiozoldine - 4 - one(K96 - 100) through its reaction with various amine and formaldehyde.The aminobenzothiazole (K1) is also used for the preparation of thiosemicarbazide (K54) by its reaction with carbon disulphide. The substituted 1,3,4 - thiadiazole (K55 - 60) is synthesized from the reaction of thiosemicarbazide (K54) with various of carboxylic acids in the presence of phosphorous oxychloride. The aminobenzothiazole (K1) is used in the preparation of aryl ( benzothiazole - 2 - ayl ) dithiocarbamate (K61 - 65) through its reaction with carbon disulphide and arylhalid or alkylhalid in ethanol.The 2 - azido - 6 - bromobenzothiazole (K66) is prepared from the reaction of sodium azide with diazonuim salt ( prepared from the reaction of benzothiazole (K1) with sodium nitrate , hydrochloric acid ). 1,2,3 - triazoline (K67 - 71) are synthesized through the reaction of 2 - azidobenzothiazole (K66) with various olefin in ethanol. The treatment of aminobenzothiazole (K1) with phenyl isothiocyanate gives (6 - bromobenzothiazole - 2 - yl) - 3 - phenylthiourea (K72) which is converted to (6 - bromobenzothiazole - 2 - yl) - 3 - phenyl - 2 - thioxydihydropermidine - 4 - 6 - one (K73) through its reaction with malonic acid in dry. Benzene. The 2 - hydrazinobenzothiazole (K80) is prepared from the reaction of 2 - aminobenzothiazole (K1) with a mixture from hydrazine hydrate and hydrochloric acid in ethylene glycol. Pyrazoline compounds (87 - 92) are synthesized from the reaction of chalcons (K81 - 86) ( which are prepared from the condensation of substituted benzaldehyde with acetophenone in a base medium ) with hydrazino (K80). The hydrazino (K80) is used in the preparation of 2 - amino( - 6 - bromobenzothiazole)isoandole - 1,3 - diaone (K93) and 1 - (2 - amino - 6 - bromobenzothiazole)pyrroline - 2,5 - di - one (K94) through its reaction with phathalic anhydride and malic anhydride in ethanol. For furthur work, the preparation of Schiff bases (K101 - 110) carried out. from the condensation of substituted benzaldehyde with hydrazino (K80) in the presence of glacial acetic acid in ethanol. The 3 - ( - 6 - bromobenzothiazole - 2 - ylamino) - 2 - (sub.aryl) - 5,7 - di - one oxazipene (K111 - 120) are synthesized by cyclazition of Schiff bases (K101 - 110) by using malic anhydride in methanol. Schiff bases (K101 - 110) are also used in preparation of 1 - ( - 6 - bromobenzothiazole - 2 - ylamino) - 4 - (sub.aryl) - 3 - chloro - 2 - oxo - zetidine - 2 - one (K121 - 130) through its rection with chloro acetyl chloride in presence of tri ethyl amine in 1,4 - Dioxan. The 3 - (6 - bromobenzothioazol - 2 - ylamino) - 2 - (substituted aryl)thiozoldin - 4 - one (K130 - 140) is prepared from the reaction of thioglycolic acid with Schiff bases (K101 - 110) in presence of zinc chloride in dry. Benzene. In order to show the antibacterial activity of prepared compounds (k11,k23,k41,k56,k61,k62,k89,k97,k112,k118,k126,k137,k139) are evaluated against four types of common bacteria (Bacillus subtillis, Staphylococcus aureus, Eschershia coli, Klebsiella peneuomina). The result of biological study are compared with standered antibiotic (Ciprofloxacine & Gentamycin). The structure of the synthesized compound are confirmed by I.R, 1H - NMR & 13C - NMR spectra and Some chemical physical data.

دراسة بعض المتغيرات الكيموحيوية على مرضى ضغط الدم

اسم المؤلف: منال عدنان ابراهيم
اسم المشرف: نزار احمد ناجي
الموضوع العام: علوم الكيمياء
السنة: 2011
الموضوع الدقيق: علوم الكيمياء
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: يتضمن البحث تحضير مشتقات جديدة للقاعدة النتروجينية البيورينية (ثيوفلين) باضافة مجموعة ازو (Azo group) الى هذه القاعدة حيث تتصل بالطرف الاخر لمجموعة الازو الحلقية حلقة بنزين معوضة بمجاميع مختلفة مثل (SO3H , Br , NO2) وقد حضرت اربعة مشتقات للثيوفيلين1 - SA | This study include synthesis of new derivatives of nitrogen bases purin (theophylline) by adding azo group to these Bases where connecting in the other terminal with Banzen ring substituted by different group like (NO3, Br,...) Four derivatives of the theophylline where these are SAT , BAT , 4NAT , 3NAT these derivatives were used as ligand because of is abitity to from helating complex , these derivatives were reacted with eight metal ions these are (Fe2+, Co2+, Ni2+, Cu2+, Zn2+, La3+, Ce3+) these complex were characterized by many methods as molarratio, measuring of electrical conductivity electronic spectra , which helped us to find the chemical structure of those complex this study also found that ratio of ligand metal was 2 : 1 this study also include the study of biological effect for these ligand and complexes on four deferent pathogenic species (staphylococcus aureus, streptococcus viridans ) ,(protens vulgaris, pseudomonas aeruginosa ) the two first species are gram positive while the other are gram negative , finally (by using disk diffusion method) it was found that these compounds show different activity of inhibition on other growth of the Becteria.

تطبيقات طريقة استخلاص سائل - سائل للفصل والتقدير الطيفي لبعض العناصر الانتقالية بواسطة معقدات عضوية جديدة Applcations of Liquid - Liqid Extraction Method For Separation And Spectrophotometric Determiination To Some Transition Metal Elements By New Organic Reagents

اسم المؤلف: احمد ابراهيم حمزة
اسم المشرف: شوكت كاظم جواد
الموضوع العام: علوم الكيمياء
السنة: 2012
الموضوع الدقيق: الكيمياء الحياتية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لمعرفة تاثير مرض ارتفاع ضغط الدم على بعض المتغيرات الكيموحيوية في الدم, ومقارنتها مع القيم الطبيعية (مجاميع السيطرة) والبالغ عددهم (125) (47 اناث و72 ذكور) والذين تراوحت اعمارهم بين (21 - 70) سنة حيث تضمن البحث : دراسة 115 حالة مرضية للاش | The present study was carried out to determine the effect of hypertension on some biochemical parameters in the blood compared to that of normal values of healthy subjects in healthy control group(125) (47 females and 72 males) Their ages ranged between(21 - 70) yearsThe study included : - 1 - seventy hypertensive patients(30 females and 40 males)Their ages ranged between (30 - 80) years the following biochemical parameters were included : (Cho, TG, HDL, LDL, VLDL, GOT, GPT, SOD MDA, HbA1C ,LDH, Metall oendopeptidase , leptin )for both hypertensive patients and health subjects as a control. Results of this study have shown a significant increase(p?0.05) in the concentration of cholesterol, triglyceride ,LDL,VLDL compared with control group while high density lipoprotein showed a significant decrease (p?0.05) compared with control groupThe estimation of LDL/HDL ratio showed a significant decrease (p?0.05) compared to that of healthy subjects The study also included the effect of hypertension duration on BMI and age in relation to the concentration of (Cho, TG ,LDL,VLDL,HDL)The study showed increase in the concentrations of (Cho, TG ,LDL,VLDL) while a decrease in the concentrations of high density lipoprotein for all patients compared with that of healthy subjects (control group).A clinical study of Metallo endopeptidase on the serum of healthy and hypertensive patients was carried which has been shown that the activity of this enzyme in healthy subjects does not affect by sex, age, and smoking while there is significant increase in the activity of Metallo endopeptidase in the serum of the hypertensive patients for both sexes at different ages compared with that of control group while there was an increase in the activity of Metallo endopeptidase in relation to the duration of disease the result also demonstrated a significant increase in the activity of lactate dehydrogenase in hypertensive patients compared with that of control group.ALT and AST enzyme levels have been estimation in this study which showed a significant increase in the level of AST in hypertensive patients for both normal weight and overweight compared with that of control group, while ALT level showed no significant difference in hypertensive patients for both normal weight and overweight compared with that of control groupAs for Leptin the study has showed a significant increase in the concentrations of leptin hormone in control group of healthy subject (over weight BMI?25) compared with normal weight(BMI?25) for the same group. the concentration of leptin in all hypertensive patients showed an increase level in relation to age &BMIThis study also included the estimation of SOD and MDA in both hypertensive patients and healthy subjects (control group).It showed a significant decrease in the activity of SOD as the duration increases and there was a significant increase in the level of MDA(P?0.05)in hypertensive patients compared healthy group.Finally the level of (HbA1C) was also studied which no significant difference in hypertensive patients compared with that of control group, while there was a signi

دراسـة بيئيــة للافقريات القاعيـة في هور الدلمج - وسط العراق An Ecological Study of The Benthic Invertebrates In Al Dalmage Marsh - Middle of Iraq

اسم المؤلف: ميسون حسن مشجل السراي
اسم المشرف: هيفاء جواد جوير
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية التنوع الاحيائي لمجتمع لافقريات القاع في هور الدلمج / وسط العراق فضلا عن بعض الخصائص الفيزياوية والكيمياوية لمياه الهور، اختيرت ست محطات لجمع العينات وذلك للمدة من بداية كانون الثاني وحتى كانون الاول 2013. استخدم تحليل التوافق CCA | Benthic invertebrate diversity on the Al - Dalmage marsh in the middle of Iraq, in addition to the some physical and chemical properties was the goal of the present study, for a period from January to the end of December 2013. Six stations along the eastern side of the marsh were chosen for monthly samples collection.The results showed water temperature ranged from(9 - 34.1)?C. The pH values were neutral but tends to near alkaline and ranged from (7.2 - 9).The Electrical conductivity values varied from (22735 - 2330) ?s/cm, according to the values of salinity (1.5 - 14.6) ‰ the marsh was classified as mesohaline (TDS) which showed high values ranged from (1820 - 17762) mg/L.This study showed that the marsh were well oxygenated due to the dissolved oxygen values which ranged from 7 - 12 mg/L, the study recorded values to BOD5 were ranging from (1.5 - 3)mg/L, and the marsh classified as very hard according to the total hardness values from (6980 - 825) CaCo3 mg/L. The high perecentage of organic matter in the sediment was 2.7 %. The results showed the station were characterized by sediment texture varied from silt clay sand sediment in the stations 1, 4, 5 , silt clay in St(2) and sand silt in St(3), sand clay silt in St(6).A total of 21286 individual benthic invertebrates was sorted, which represents which 78 taxonomic units, including 9 taxa. The Crustacea was the dominate group representing 29% of the total number of benthic invertebrates. It was noted that there were obvious differences in the total number of sorted individuals in which varied in each location and each month. The highest total number recorded in was st (2) was 4360 including 73 taxonomic units, while the highest total number recorded in April was 2624.The results of The relative abundance index showed that the species insects : Polypedilum sp Were more abundant in St(2 - 6), and dominant in St(6), while the Seinura sp were dominant in St(6), while the other taxonomy units varied from less abundant to rare abundant. Also the results of constancy index showed The which were considered "constant " However the other taxonomy units varied from "accessory " and" accidental" taxonomy units in study stations.The Shanon - Weiner index of totall benthic invertebrates varied from (1.2 - 3.8) bit/Ind, The uniformity index of benthic invertebrates varied from(0.7 - 1), these high values indicate that there is no ecological stress on benthic invertebrates in the study area.The Results of Jaccard Presence - community of similarity showed the highest degree of similarity to be 96% which recorded between St(1) and St(2).The values of the index of spcies richness varied from 1.3 - 15 with the greatest values recorded in April while lower values were in October.Six species of Turbellarian Platyhelminthes that were recorded, from which Stenostomum leucops, Stenostomum bryophilum, Stenostomum unicolor, Gyratrix hermaphrodites, Macrostomum sp, Dalyelliidae, which were considered as new records for Iraq, with Gyratrix hermaphrodites as the most abundant species and representing 25% of the total number of Turbellarian. It was noted that there were obvious differences in the total number of sorted individuals which varied in each location and each month, the highest total number recorded in St 3 was 400, while the highest total number recorded in November was (198).The present study result showed that three species of Nematoda were recorded incuding Seinura sp, Alaimus sp, Dorylaimus sp, Seinura sp as the most abundent species representing 84% of the total number of Nematoda. Spatially the highest total number of 590 individuals was recorded in St (4) , while temporally, the highest total number of 366 was recorded in October.One unidentified species of Gastrotricha was recorded for the first time in Iraq, 102 Ind were sorted these were considered "constant" recording to constancy index 63.8% which were sorted from all stations.A total of 2319 individuals of Rotifera was sorted, these were identified as 15 species that included Philodina sp, Rotaria citrinus, Dipleuchlanis sp, Euchlanis sp, Notholca sp, Ttriploeuchlainis sp, Colurella sp, Lepadella sp, Lecane sp, Proales sp, Cephalodela sp, Synchaeta sp, Hexarthra mira, and finally Brachionus sp which was recorded the highest number of individuals 540 individuals.A total of 1888 individual Annelida were sorted representing 12 species including nine species belonging to the family Naididae (three belong to the sub family Naidinae and six belonging to Tubificoid worms), two species belonged to Aleosomatidae including Aeolosoma hemprichi, Aeolosoma variegatum, and finaly one specie belong to Hirudinea which was Glossiphonia heteroclite, with three 3 ind in all study.The family Aleosomatidae recorded the highest percentage of 58% of the total number of Annelida.The sub family Naidinae including Paranais litoralis, Dero (Aulophorus) furcata, Stylaria lactustris.The highest frequency of 23.6% was recorded by P. litoralis.The Tubificoid worms represent six species which were Limnodrlius hoffmeisteri, Limnodrlius profundicola, L. claparedeianus, L udekemianus, Tubifex tubifex, Branchiura sowerbyi. This study also isolated 6238 individual Crustacea, these were identified as 15 species that included five species belonged to Cladocera, four order belong to copepod, which were recorded the highest percentage 30% of the total number of Crustacea, in addition to Macrobrachium nipponense from Decapoda, Sphaeroma annadalei anndalei from Isopoda, Ostracoda, Amphipoda, Conchostracha. It was noted that there was obvious differences in the total number of sorted individuals which varied in each location and each month, The highest total number recorded in st (2) was 1480, while the highest total number of 730 was recorded in April. The study finds 4297 individual insects groups, were sorted represent seven order includes larvae of Ephemeroptera, Trichoptera, Odonata, (Zygoptera, Anisoptera) Diptera (Culicidae, Chironomus sp, Polypedilum sp) Lepidoptera larvae Hemiptera (Corixidae, Nepa cinerea), and adult of Coleoptera (Dysitidae, Hydrophilidae Elmidae, Curculionidae, Coleoptera larvae), the Diptera larvae were recorded the highest percentage 55 % of the total number of insects groups, and Nepa cinerea was considered as new records for Iraq.This study showed two class from Mollusca, 1956 Ind were sorted, including Gastropoda and Pelecyopoda.The Gastropoda which were recorded the highest percentage 94% of the total number of Mollusca, these were identified to physa acuta, Lymnaea sp, Melonoides tuberculata, Melnopsis nodosa, Theodoxus sp, physa acuta which was the greatest percentage 41% of the total number of gastropoda. pelecyopoda which were including Pseudodontopsis euphraticus, Tigridis Unio, Corbicula fluminea, Corbicula fluminali

دراسة تشخيصية جزيئية ودمية وكيموحيوية لانواع الاكريات Eimeria spp. في الدجاج المصاب طبيعيا وتجريبيا في محافظة الديوانية Molecular Diagnosis Study And Haematological And Biochemical of Coccidiosis Eimeria Spp. In Natural And Experimental Infection Chicken In Al - Diwanyia City

اسم المؤلف: خالد ثامر مطر الشيباني
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في البيت الحيواني التابع لقسم علوم الحياة / كلية التربية / جامعة القادسية للمدة من 1/9/2013 ولغاية 1/9/2014، للتحري عن انواع طفيلي الاكريات المتطفلة على الدجاج المصاب طبيعيا وعزل الطفيلي من الحالات المصابة الواردة الى المسستشفى البيطري | The study conducted in The animal house of the Biology department, College of Education, University of Al - Qadisiyah during the period from 1/9/2013 to 1/9/2014. The study diagnosed the species of Eimeria parasites in naturally infected chickens in poultry farms. Eimeria oocysts was isolated from the infected chickens feaces in Veterinary hospital and Veterinary clinics.Then, the cases diagnosed initially in the laboratory by direct examining stool smears and the positive cases were recorded.Through this study, oocysts were isolated, and two species of Eimeria parasites were diagnosed (E.tenella and E.maxima) by using the technique of Conventional Polymerase Chain Reaction technique.There are 186 cases of infected chicken which examined, and the results showed that (186 out of 315)stool Samples(59%), include infected with two species of Eimeria parasites E.tenella was in (135 out of 186, 72.5%) while the species E.maxima was in(51 out of 186, 27.4%). The experimental study was conducting two experiments were done.The first experiment, depend on the experimental infection of E.tenella species which was used for two types of chicken, (Ross type which is foreign, and local breed).The second experiment, includes experimental infection with the species E.maxima also in two types of chickens that mention above. Symptoms and clinical signs of the first experiment on foreign chickens included were recorded it is noticed that infected the chickens with E.tenella have was dull, unorganized feather and loss of appetite in addition to bloody diahrreha, loss weight, emaciated and isolated. The Gross Lesions include congestion of cecum and an increase in their sizes while inside the cecum, many ulcerated lesions were noticed, filling cecum with mucus and blood, liver was congested and enlarged. Microscopicale examination recorded congestion was full with blood cells and necrotic tissues cecum and falling of cells in the cecum lumen. Many different stages of the parasites were noticed as schizonts, merozoites, gametes and oocysts Hematological parameters showed high significant decrease (p?0.05) in Hb (0.38±5.94 mg / 100ml) in the fourth week of infection, PCV (0.68±18.47%) in the fifth week and RBC was (1.58±0.02x106) in the fourth week. Also, the infection led to significant increase difference of WBC (30.19±0.95x103) in the seventh week of infection in comparison of uninfected chickens of the control sample. The biochemical parameters of blood serum showed that significant decrease (p?0.05) in glucose concentration (156.98±1.75mg / 100ml in the fourth week of infection), cholesterol concentration and total protein concentration (62.11±4.40, 1.92±0.92 mg / 100ml respectively in the fifth week of infection).Also, Uric acid increased significantly (p?0.05) to reach(10.19±0.52mg / 100ml) in the fourth week of infection in comparison of uninfected chickens of the control group. Average weight was showed the significant decrease (p?0.05) of the chickens quantity (368.88±3.01gr in the fourth week of the infection), and an increase the percentage of mortality(23.33±0.23% in the fifth week of infection), addition to the decrease of consuming food by bird (282.8±2.19gr in the fifth week of the infection) in comparison of uninfected chickens of the control group. While the results of local breed chickens in the first experiment were less severity than that with the foreign type (Ross) but the colour of the stool was brown mixed with blood. The Gross Lesions showed pathological changes in the cecum as congestion and bleeding. Moreover, ulcers areas were smaller and less in number in comparison with the infected foreign chickens by the same parasite. The microscopic pathological changes of E.tenella in the cecum showed hyperplasia in goblet cells and congestion of blood capillaries with less number of stages of the parasite in comparison with the foreign chickens. The hematological parameters showed significant decrease (p?0.05) in Hb concentration and percentage of PCV (7.13±0.31mg/100ml, 21.26±1.82% respectively in the fifth week of infection), while the RBC count was (2.05±0.12 x 106 in the fourth week of infection and significant increased (p?0.05) in WBC count was (30.63±0.38 x 106 the sixth week of infection) in comparison with the chickens of the control group. The biochemical parameters of blood serum showed the significant decrease (p?0.05) in glucose concentration (169.60±2.16mg/100ml in the fifth week of infection), cholesterol concentration and total protein concentration (70.89±2.01, 2.25±0.39mg/100ml respectively in the fourth week of infection). Uric acid increased significantly (p?0.05) to reach (7.62±0.90mg/100ml) in the fifth week of infection in comparison with the control group. Average of the weight was significant decrease (p?0.05) (190.28±3.81gm), high increase in the average of mortality (10±0.20%), and high decrease in the consuming food average (413.4±3.91gr) in the fourth week of infection in comparison with the uninfected control group. The second experiment of the foreign chicken which infected experimentally by E.maxima showed asymptoms and clinical signs were nearly similar in the foreign chickens that infected with E.tenella, but mild in severity is average. The symptoms include fatigue, fallen wings, loss of appetite, mucus diarhea mixed with blood. The Gross Lesions showed severe changes in the infected part of intestine as congestion and filling of intestine with mucous secretion of bad odour. While the microscopic pathological changes showed the tissues section of intestine were damaged, necrosis of intestine villi due to the large number of different stages of the parasite with hyperplasia of goblet cells. The hematological parameters showed significanty decrease (p?0.05) in Hb concentration, percentage of PCV and RBC count (7.55±0.61mg/100ml, 23.62±0.81% and 2.08±0.10x106 in the fourth week of infection) and significant increased (p?0.05)in WBC count (31.26±0.63x106 in the seventh week of infection) in comparison with the uninfection control group. The biochemical parameters of blood serum showed that there was the significant decrease (p?0.05) in glucose concentration (164.86±2.11 mg /100ml in the fifth week of infection), cholestrol concentration and total protein (77.14± 3.38, 2.24±0.36 mg/100ml) and significant increased (p?0.05) in Uric acid concentration (8.20±0.79mg/100ml) in the fourth week of infection in comparison to the control group. The results of productive characteristics refered to significant decrease(p?0.05) in the weight of chickens (479.28±0.23gr) and significant increase (p?0.05) in mortality (10±0.11%) during the fifth week of infection. Also, there was significant decrease (p?0.05) in the consumed food by birds (368.5±2.10gr) during the fourth week of infection in comparison to the chicken of the control group. While the results of local breed chicken in the second experiment, the symptoms and clinical signs showed no sign of disease, and the chickens were active in spite of presence of infection and the releasing of Oocysts. The Gross Lesions showed the inflammation and congestion, onlargment of the infected part of small intestine, little mucous and liquid inside the lumen.As far as microscopic changes are concerned, the tissue examination showed some necrosis and damage of the villi ends and an increase in the size of the goblet cells. Hematological parameters showed to the significant decrease (p?0.05) in Hb concentration and percentage of PCV (7.86±0.45 mg/100ml, 23.60±0.53% respectively during the fifth week of infection), RBC count (2.10±0.13 x 106 in the fourth week of infection) and significant increased (p?0.05) in WBC count (32.98±0.58 x 106 during the sixth week of infection) in comparison with the control group. The biochemical parameters of blood serum showed that there was the significant decrease (p?0.05)in glucose concentration and cholestrol concentration (170.60 ±2.01, 79.70±2.69 mg/100ml respectively in the fourth week of infection), total protein concentration (2.42±0.31mg/100ml in the fifth week of infection) and significant increased (p?0.05) in Uric acid concentration (6.52±0.82mg/100ml in the fourth week of infection) in comparison to the uninfection control group. Average of the weight referred to significant decrease (p?0.05) of chickens (213.16±3.12 gr) and consuming food (481±3.21 gr) during the fourth week of infection.While there were no differnces in the percentage of mortality comparison with the uninfected chicken of the control group. Also, the results of the number of released oocysts showed significant increase (p?0.05)in number of released oocysts with stool by infected foreign ckicken(Ross) in comparison with the local breed chicken which infected by the same parasite.Also, the number of the dropped oocysts of E.tenella were higher than that of E.maxima.

دراسة بعض جوانب الاداء الحياتي لبعض الحشرات ذات الاهمية الجنائية في مدينة الديوانية A Study of Some Biological Aspects of Forensically Important Insects In Al - Diwaniya City

اسم المؤلف: عباس كاظم حمزة الشيباني
اسم المشرف: سعدي محمد هلال
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الحشرات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The present study was aimed to identify the most forensically important insects species in addition to their distribution and abundance in the Al - Diwaniya province, also the succession pattern on carrion in urban and rural areas in different seasons was studied. The present study included some experiments on the effect of the quality and quantity of food on the development of some blow flies, the effect of rearing on food spiked with different concentration of Paracetamol and Amitriptyline drugs on some biologicalaspects of the blow flies, The result showed the following : ? When using fly sex - attractant poisoned bait used in the field, six dipterans flies were identified : 1 - Calliphora vicina 2 - Lucilia sericata 3 - Chrysomya albiceps 4 - Ch. Megacephala 5 - Sarcophaga africa 6 - Musca domestica the last species was the most abundant during all seasons of the year. However, when natural food bait was used, the same species of flies were recorded in addition to three species of beetles : 1 - Dermestes maculates 2 - Saprinus semistriatus 3 - Necrobia rufipes. the results also showed that the daily activity of different flies species was the varied according to ambient temperature during the day andthere was no activity recording for the flies during the night. ? The succession of insects on rabbit carrion in the urban and rural area in winter season showed that the most abundant fly species attacking the carrion during the active stage, these flies were : : 1 - C. vicina 2 - L. sericata 3 - Ch. albiceps 4 - Ch. megacephala in addition to S. africa, M. domestica and three beetles namely : 1 - D. maculates 2 - S. semistriatus 3 - N. rufipes especially during the late stage of decaying, and recoded fly : Eristalis aeneus (Diptera) accidentally appeared on the carrion. However, less number of fly species were recorded on rabbit carcass during summer season.? In general the present study showed fly species : C. vicina and L. sericata Their numbers in urban area were more than the rural area (urban species) while fly species S. africa was rural species. ? 4) skeletonization time of the rabbit carcass during summer season was three times faster than it was in winter season in both locations, urban and rural.? When C. vicina larvae reared on different animal tissues, the result showed that there were a significant differences between means of larvae weight and development time and in the emergence of adults from pupal stage, thus, the average weight of larvae was 2337 mg 2101 mg when reared on lung and liver tissues as a compared with 998.6, 1194 and 1278 mg when reared on kidney, brain and muscles after eight days of exp - Time. However the longest development period of larvae were : 10.6, 10.3 and 9.3 days and pupae 7.3, 7 and 6.6 days when reared on kidney, brain and muscles respectively, as a compared with : 7.3, 7.6 and 8 days(larvae) 5.3, 5.6 and 6 days (pupae) reared on lung, liver and heart respectively (P? 0.05).The highest emergence percentage of adult were 46.6% and 40% in the lung and liver tissues. ? Larvae of C. vicina reared on 25 and 50gm beef liver /10 larvae were 83.2 and 75.6 mg respectively compared with 67.1 reared on 100gm/10 larvae during the first four days of the experiment. However, the duration period of larvae and pupae were 10.3 and 8 days when reared on 100gm respectively, the highest emergence percentage of adults were 46% and 26.6% for larvae reared on 25 and 50gm of liver. ? Larvae of C. vicina reared on spiked liver with different concentration of Amitriptyline were significantly different to those in the control treatment, the highest means weight of larvae was 1615.3 and1474 mg when reared on liver spiked with 1.25 and o.125mg ofamitriptyline/25gmliver/5larvae respectively the lowest weight mean of larva was 1112 and 1264.6mg when reared on liver spiked with 0.025 and 0.0mg(control) respectively. The development period of larvae and pupa of C. vicina were 14.6 and 10.3 days respectively when reared on liver spiked with 1.25mg of Amitriptyline compared with 9.6 and 6.3 days in the control treatment(p>0.05).The highest percentage of adults emergence 40% was in the control treatment compared with only 13.3 when larvae reared on liver spiked with 1.25mg.amitriptyline. ? Larvae of C. vicina reared on beef liver spiked with different concentrations of Paracetamol showed different results. The highest mean weight of larva was 1735.3mg when reared on beef liver spiked with 25mg/5 larvae compared with 867.3mg in the control treatment, the development period of larvae and pupae were 12.6 and 8.6 days respectively as a compared with 9.3 and 6.3 days in the control treatment. The percentage of adults emergence was 46.6% in the control treatment compared with 13.3% in the highest concentration of drug ? The results also showed that there is a significant increase in the mean weight of larvae reared on different tissues of rabbits oral dosage with lethal dose of Amitriptyline, in the contrary weight of larvae reared on tissues of rabbits orally dosage with lethal dose of Paracetamol were decreased(p>0.05) as a compared with untreated rabbit tissues (rabbit was not dosage - control).

دراسة وبائية للانماط الجينية لطفيلي الاميبا الحالة للنسيج والجيارديا الامبيلية في منطقة الفرات الاوسط العراق

اسم المؤلف: صبا فاضل علي مهدي معلة
اسم المشرف: علي حسين مكي الكبيسي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: This study was conducted on the 1350 stool samples from diarrhea - suffering patients in three provinces of middle Euphrates region : Karbala, Babel and Al - Najaf (450 samples in each province). Patients ranged in age from 1 to more than 60 years and from both sexes and different regions who were attended to some hospitals, dispensaries and private laboratories in these provinces during the period from the first of May 2013 till the end of April 2014. All collected fresh samples were examined microscopically by wet - mounts method for G. l a mbl i a and E n ta m oeba histolytica detection One third of positive samples in microscopic examination (which was selected to be representative for all positive samples) from each province and ten of negative one as control group were submitted to the nucleic acid (DNA) extraction. Molecular diagnosis of G. l ambl i a and E. h i s to l yti ca were performed using PCR reaction with amplification of the 532 - bp and 355 - bp fragments from the internal transcribed spacer region1 ITS1 for each parasite respectively. The eluted DNA of each positive sample in ITS1 test with its specific primer was also submitted to a traditional PCR and RT - PCR techniques for detection two genes in G. l a m b l ia { Glycoprotein A Repetitions Predominant (GARP) and Adenosine Triphosphate Guanine Tri Phosphate (ATP/GTP) } and two genes in E. histolytica {Acetatekinase (ACK) and Methionine GammaLyase (MGL)} using PCR and RT - PCR techniques. Five positive PCR products in ITS1 test of G. la m blia were used for sequencing. Based on the results of this study, microscopic examination showed that the total percentages of infection with G. l am bl ia were (14.7, 17.3, 16) % and with E. histolytica were (15.1, 13.3, 16.7) % in Karbala, Babel PDF created with pdfFactory Pro trial version www.pdffactory.comand Al - Najaf respectively. The infection with G. l am bli a and E. histolytica was more frequent in males than in females and the prevalence of both parasites in rural areas was higher than the urban areas P < 0.05 in all studied provinces. The highest incidence of infection with G. lamblia occurred in age group 11 - 20 years (P < 0.01) and that with E. histolytica occurred in age group (1 - 10) years (P < 0.05) in all studied provinces. This study was revealed uneven percentages of infection with both parasites during different months of a year (P < 0.01), the highest rate of infection with G. lamb l ia and E. h i s t o l y ti ca were in summer. For the molecular diagnosis of G. l a mbl ia and E. hi s t ol y t i ca, using PCR technique, the prevalence of G. l amb l ia and E. his t o l y ti c a in middle Euphrates region were found to be 14.9 % and 12.8 % respectively. The infection with G. l a m b lia and E. hi stol y t i c a was more frequent in male (62.7, 63.8) % than in female (37.3, 36.2)%, the prevalence of the parasites in rural area (67.2, 69.0)% was higher than the urban area (32.8, 31.0) %, the highest incidence of infection occurred in age groups 1120 and 1 - 10 years with the percentages of (28.4, 29.3) %, the highest rates of infection were in August (20.9%) and July (19.0%) respectively. Significant differences were found between the rate of infection with both G. l a m bl i a and E. hi stol y t i ca and the different parameters (gender, residence, age gropes and months of a year). The results of detection the gens (GARP, ATP/GTP, ACK, MGL) by using PCR technique showed that the total percentages of positive samples were (55.0, 70.0, 45.0, 75.0) %, (62.5, 79.2, 47.0, 70.6) % and (65.2, 73.9, 61.9, 71.4) % in samples of Karbala, Babel and Al - Najaf respectively while those percentages by using RT - PCR were (60.0, 80.0, 50.0, 80.0) %, (66.7, 83.3, 58.8, 82.4) % and (65.2, 78.3, 66.7, 71.4) % respectively. For the detection of genes in G. l am bl ia, sensitivities of 91.8 % and 97.8 % and specificities of 94.6 % and PDF created with pdfFactory Pro trial version www.pdffactory.com81.4% for PCR and RT - PCR respectively were observed when compared to each others, While Sensitivities of 89.9 % and 98.6% and specificities of 97.3 % and 81.8 % for PCR and RT - PCR respectively for the detection of genes in E. hi sto l y t i c a were observed when compared to each others. Multiple sequence alignment analysis and phylogenetic analysis results of this study demonstrated that the G. l ambl ia in Middle Euphrates region - Iraq isolates had nucleotide homology sequence identity percent of 99% with Giardia intestin a l i s (AF199448.1) Australia isolate and with USA isolate Giardia microti

التشخيص الجزيئي وخصوصية المضيف لداء الابواغ الخبيئة Cryptosporidiosis في بعض انواع الطيور البرية والداجنة ود راسة الاصابة التجريبية في الفئران البيض Molecular Diagnosis And Host Specificity For Cryptosporidiosis In Some Species of Wild And Domestic Birds And Study of Experimental Infection In White Mice

اسم المؤلف: اخلاص عباس مرهون الزبيدي
اسم المشرف: غيداء عباس جاسم الجبوري
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The present study aimed to investigate of Cryptosporidiosis in some wild and domestic birds, and study some concerned aspect of it such as : Host specificity for Cryptosporidium spp., histo - pathological changes which parasite produced it and molecular analysis for this species. To attainment this purpose we applied our study in three major parts : The First part : Investigation of Cryptosporidium in birdsA total of 236 birds were collected from different regions of Al - Diwaniya province between May 2013 to June 2014, Birds included six species which are : 22 sample from Turkey (Meleagris gallopova), 60 from Quail (Coturnix coturnix) , 40 from Domestic chicken (Gallus gallus domesticus), 52 from Caged chicken(Gallus gallus), 32 from Common duck (Anas platyrhynchos) and 30 from Feral pigeon (Columba livia). Fecal and bile samples which took from birds were examined by used hot modified Zeihl - Nelseen stain.Results revealed that the birds were infected with two types of Cryptosporidiosis : Intestinal and Biliary Cryptosporidiosis (58.1% and 12.7% respectively).Our study recorded four species belong to Cryptosporidium be responsible for intestinal type which are : C.parvum, C.meleagridis, C.baileyi and C.galli, While just the first three species of them were be responsible for biliary type.Also results showed that no significant difference according to gender of birds in infection with two types of Cryptosporidiosis, while showed a significant difference to study's months in infection, the highest ratio of Intestinal type was in March and April (87.5% and 89.3% respectively) and the lower ratio was in July, it reached 27.8% ; While the highest ratio of biliary type was in April, it reached 39.3%.Also results revealed that all cases of Biliary type were light infection, while the severity of infection with Intestinal type was between heavy and light infection. Quail birds showed a significant difference in severity infection compared with other birds, it was higher in quail which reached 60.9% and lower in common duck(30.0%) and there was no heavy infection in feral pigeon. when reading of preparation histological sections from : intestinal, fabricious bursa and bile sac, the results showed a histological changes that parasite induced it which included : hyperplasia in infection's regions, necrosis and destroyed in infected cells with infiltration of inflammation cells.The second part : Experimental studyTo test a host specificity and to know how incidence of cross transmission of Cryptosporidium species which isolated from birds to human, therefore the laboratory mice were dosage with the four species of parasite and then we determinate of : susceptible of age group to infection, prepatent period and histological changes in experimentally infected mice.Result showed that cross transmission could happened for three species of Cryptosporidium that infected birds to lab. mice which are : C.parvum, C.meleagridis and C.baileyi with total ratio 30% and showed a significant difference compared with control group, and the results recorded a mortality ratio in infected mice (7.5%).The probability of mammalian infection by Cryptosporidium spp. which isolated from birds in our study represent the first record in Iraq. C.parvum and C.meleagridis showed loss of host specificity whereas C.baileyi was opportunist in his ability to infect another host beside their ordinary host (birds), while C.galli showed a high specificity for its host.The third part : Molecular studyThis part in our study studied for the first time in Iraq, which contains the diagnosis of Cryptosporidium species that infected birds and their genetic analysis through : DNA sequence analysis of species by used small subunit ribosomal RNA gene (18SrRNA), read of phylogenetic tree of these species and do comparison among genetic trees according to : original host (birds), experimental host (infected mice) and to their parasitic site in their ordinary host.The phylogenic tree of parasite species was drown by used MEGA6 program, the results of neighboring tree revealed two major branches : First branch include C.galli while the second branch include the other three species. Also genetic tree showed presence of two difference strains of C.parvum can infected the birds with neighboring ratio between them reached to 82% and the same thing was found for C.meleagridis but with neighboring ratio reached to 70%.The reading of phylogenetic tree showed that all species of studied birds can infection with isolated Cryptosporidium species excepting C.galli which just isolate from domestic chicken and C.meleagridis which found parasite only in turkey and quail.Results of genetic analysis of neighboring tree among species according to parasitic sites in infected birds showed similarity between species which isolated from fecal and bile, which are : C.parvum, C.meleagridis and C.baileyi, this result corroborate the ability of these species to transfer their infection from intestine to bile sac.The results of comparative concerned with isolated species from infected mice's fecal(experimental host) and from birds' fecal (ordinary host), results showed similarity among three species which isolated from mice's fecal with their analogous species isolated from birds' fecal which are : C.parvum, C.meleagridis and C.baileyi, while C.galli doesn't have ability to infect mice which dosage it.Finally the result of this study give evidence to ability of cross transmission for these three species from the class : Aves to the class : Mammalian, So this fact give to us a hygienic indication for probability of human infection with these species.

دراسات وراثية خلوية ومظهرية لثلاثة انواع من شبوطيات المياه العذبة العراقية (Barbus luteus, Cyprinion macrostomus, Chondrostoma regius) Three Species of Iraqi Freshwater Cyprinidaes (Barbus Luteus, Cyprinion Macrostomus, Chondrostoma Regius)

اسم المؤلف: اسماء سامي ابراهيم
اسم المشرف: ندى عبد المجيد الانصاري طه ياسين الدوري
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الحيوان - الوراثة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية التعرف على الطراز الكروموسومي ونظام تحديد الجنس والتمييز بين الجنسين مظهريا في ثلاثة انواع من اسماك المياه العذبة العراقية ممثلة بسمكة الحمري Barbus luteus والبنيني كبير الفم macrostomus Cyprinionوالبلعوط الملوكي Chondrostoma regius | These present studies were carried out to determine the karyotypes & the system of sex determination between the two sexes as well as the sexual morphological characteristics of three species of Iraqi fresh water fish Barbus luteus, Cyprinion macrostomus, & Chondrostoma regius that were fished from Tigris river at Baghdad during 2005. The cytogenetic studies revealed that the chromosome number of Barbus luteus is 2n = 148 & the karyotype of males is (80m + 52sm + 16st) while the females has (80m + 51sm + 17st). The chromosome number of Cyprinion macrostomus is 2n = 50 the males has (6m + 24sm + 12st + 8t) while the females has (6m + 23sm + 13st + 8t). The chromosome number of Chondrostoma regius is 2n = 48 and the karyotype is (14m + 30sm + 4st). Sex chromosomes were identified in Barbus luteus & in Cypinion macrostomus for the first time , the sex is determined by ZZ / ZW system, the female was considered as heterogametic (ZW) while the male was considered as homogametic (ZZ) , & the chromosome Z was represented as submetacentric chromosome & chromosome W as small subtelocentric chromosome. In spite of the difference in their chromosome number , the ratio between red blood cell diameter & it's nucleus was approximated in these species. The differences among these species in both of the diameter of cell and the nucleus didn't match with the differences at the polyploidy levels or with the difference in chromosome number within the same level. The liver & intestine were recommended as new sources for chromosomal studies , because of the good ratio of dividing cells (71.4 % & 58.2 %) respectively from the whole dividing cells in kidney that was considered as a control. The crude aqueous extract of fresh convolvulus arvensis leaves showed good effect in arresting the cell division of fish at metaphase , the concentration (0.4, 0.6, 0.8, ) mg / gm arrested kidney cells of carp fish at the metaphase in ratios 71.1 % , 88.1 % , 94 % respectively from the colchicines treated kidney cells (control). Positive significance correlation was found (r = 0.9518) between the percentages of arrested cells at metaphase & the concentrations of crude extract. Comparison between the females and males of the three species revealed that in Barbus luteus the distance from head edge to the anal orifice & from head edge to the dorsal fin were larger in males than females. The females have dark colored fins with reddish ventral aspect of head & body in comparison with the males during the whole months of study. So , they were considered as permanently sexual dimorphism characteristics. But , during the spawning seasons (May & June ) a more brighting color of male with reddish coloration of both sides of body were noticed in comparison with female with a golden orange corneal coloration in both sexes. Thus, they were considered as temporary sexual dimorphism characteristics. In Cyprinion macrostomus the distance from the head edge to the anal orifice was larger in females than males. The colors of anal & caudal fins were darker in females than in males during the whole monthes of study.So , they were considered as perminantly sexual dimorphism characteristics. While, during the spawning seasons ( May , June , July & August) , the gill operculum was of orange color only in male that extend to the end of pectoral fin. So , this was considered as a temporary sexual dimorphism characteristic. In Chondrostoma regius the distance from the head edge to the dorsal fin & from the dorsal to caudal fin as well as the pectoral fin base length were larger in females than in males during the whole months of study it was considered that they are permanently sexual dimorphism characteristics. Differential characteristics were not noticed during the spawning season (February & March). The permanently sexual dimorphism & the general characteristics were not affected by the differences of standard length , weight , age & season , because they were observed in fishes of different lengths , weights & ages & during the months of study 2005. But , the seasons affect the development of temporary characteristics in Barbus luteus & Cyprinion macrostomus , because these characteristics were observed during the spawning season only. Aging of Barbus luteus was accompanied by growth increase as indicated by the mean of standard length during May , July, August, September & December & by the mean of weight during May , August, September, October & December. A positive correlation was found between standard length & weight during the months of study , with the exception of January. While aging of Cyprinion macrostomus & Chondrostoma regius was not accompanied by growth increase as indicated by the means of standard length & weight , but a positive correlation between standard length & weight was found during the studied months. Same growth type were observed in the three species of fish , it was allometric in two sexes.
1 ... 695 696 697 698 699 ... 962