اسم المؤلف:
اسيل كريم جبار السلطاني
اسم المشرف:
هادي مزعل خضير الربيعي
المستخلص:
اجريت سلسلة من التجارب المختبرية في مختبر بيئة الحشرات/كلية العلوم للبنات/جامعة بابل خلال عام 2014 - 2015 تضمنت اختبار فعالية مستخلصات المركبات التربينية والقلوانية والفينولية الخام لنبات الزريج Chrozophora tinctoria في بعض معايير الاداء الحياتي لحشرة الذ | A series of laboratory experiment were conducted in the laboratory of insect /college of Science for women /University of Babylon during the year 2014 - 2015 The present study conducted to evaluate the activity of crude extract of Terpenoid, Alkaliod and Phenolic compounds of Chrozophora tinctoria on some biological aspect of Musca domestica and histological study for effect crude phenolic compound on ovaries as well as isolation and identifaction of active compounds in these extracts.The extract of crude terpenoid compound was more effective than crude alkaloid and phenolic compound in different critirra of biological perphormance. The mortality rates of eggs in the extract of terpenoid alkaloid and phenolic compound at concentration 1mg/ml were (90, 63.7, 39.6)? respectively compared with (0.4, 0.8, 0.8)% in the control tretmentsThe mortality rate of first larval instar was (57.64, 57.00, 26.85)% respectively when treated with crude terpenoid, alkaloid and phenolic compounds at concentration 1mg/ml compared with (0.8, 0.8, 0.8)% in the control treatment.The mortality rate of pupae was 18.06% when treated with crude terpenoid extract at concentration 1mg/ml compared with 0.4% in the control treatment. The cumulative mortality rates of immature stage when treated with crude terpenoid, alkaloid and phenolic compound were (100, 100, 42) % at concentration 1mg/ml compared with (15, 14, 12)% at the control treatment.Developmental period of immature stage was 36.6 days when treated with crude alkaloid extract at 0.25 mg/ml compared with 10.2 days in the control treatment.The pupal weight and female productivity were reduced from 0.1gm, 122.6 egg/female in control treatment to 0.556 gm, 0 egg/female respectively when treated with crude alkaloid extract at 0.25 mg/mlThe different concentration of crude terpenoid, alkaloid and phenolic compound were effective in the performance of eggs deposition, the nummber of eggs decreases in crude extract terpenoid, alkaloid and phenolic compounds at the concentration in same space was (0) egg/femal for all extracts at concentrion 1mg/ml comparted with (117, 118, 106) egg/femal in the control treatment, while was (0) egg/femel when treated in different concentration of terpenoid, alkaloid alone of consentration 1mg/ml compared with (120, 122) egg/femel in the control treatment.The number of eggs was (37) egg/femel when treated with phenolic compounds at same concentration compared with (120) egg/femel in the control treatment.The study included the effect extracts activity for crude phenolic at concentration 1mg/ml in histological structure for insect overies, The length and diameter of ovary and ovares follicles at (24, 96) houres after emergency while the length and diameter of ovary was (625, 468.75)µm respectively at 24 houre at concentration 1mg/ml compared with (652.173, 565.217)µm in the overy of femel in the control treatment. The length and diameter of ovaries follicles were (116.66, 72.22)µm respectively in the femel overy for control treatment, while was not distingwish for follicles female which were produced from treatment. while in (96) hour after emergency the length and dimeter of ovary (1114.285, 971.428)µm respectively when treated with extract compard with (1688.88, 1481.48)µm for overy of female in control treatment.Isolation and identification of active compounds in the crude extract of terpenoid, alkaloid and phenolic compounds, of Chrozophora tinctoria by using high performance liquid chromatography (H.P.L.C), Five compounds isolated from crude terpenoid extract was Teter methyl hydrazine compound which had retention time(1.333) mintue which was the first compound, while seven compounds isolated from phenolic compounds The gallic acid compound had retention time 1.34 mintue, which was the first compound isolation.Two compounds were isolated from crude alkaloid compounds which were berberine and isoquinoline, berberine compound had retention time 2.51 mintue which was more concentration than other compound.
