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التهاب الامعاء التنخري في الدواجن الامراضية, وطرق السيطرة والعلاج == Necrotic Enteritis In Poulry Pathogenesis, Prevention And Curative Measures

اسم المؤلف: سهى نجم الربيعي
اسم المشرف: علاء عبد العزيز عبد
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

الكشف الجزيئي والمصلي لداء المقوسات في الانسان والاغنام في محافظة واسط == Molecular And Serological Detection of Toxoplasma Gondii In Human And Sheep In Wasit Province

اسم المؤلف: عباس حسن خلاطي السراي
اسم المشرف: نعمان ناجي عايز
الموضوع العام: الطب البيطري
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحاليه خلال الفترة من تشرين الاول 2013 الى ايار 2014 في محافظة واسط للكشف عن الاصابة T. gondii في الانسان والاغنام باستخدام الطرق الجزيئية والمصلية. تم جمع خمس مئة وثمانية عينات 500 عينه دم و8عينات من انسجه المشائم و305 عينه 300عينه دم و5ع | The present study was conducted during the period from October 2013 to May 2014 in Wasit province to detect the infection of T. gondii in women and sheep using Molecular and Serological methods.Five hundred and eight samples 500 blood samples and 8placenta and 305samples 300 blood samples and 5placenta were collected from both suspected women and sheep respectively. The sera samples were separated and examined by ELISA for human and Latex agglutination test for sheep to detect the infection with T. gondii serologically, then many blood and placental tissue samples (89 blood samples and 8 placental tissue samples for women, whereas 100 blood samples and 5 placental tissue samples for ewes) were subjected to polymerase chain reaction (PCR) technique to detect the infection molecularly.The Serological results showed that 17.8% of women were positive for the private screening ELISA detects toxoplasmosis (17 % with chronic infection and 0.8% with acute infection), it was noted that the highest rate of infection was in women who ranged in age between 20 - 29 years, reaching 19.9%, but with no significant difference (P>0.05) between the ages studied. On the other hand the rate of sheep infection by latex agglutination test with 2 - mercaptoethanol amounted to 33.33% (31.33% with chronic infection and 2% with acute infection) and most positive cases were among the ages which were equal to or more than 3 years (?3 years) with significant difference (P<0.05) between these ages. The present study indicated a lack of months effect on the distribution of parasite infection rates where these different months recorded relatively close rate ranged between 14.45% - 23.07% in women and 31.42% - 35.97% in sheep with no significant difference (P>0.05).Regarding to polymerase chain reaction test, a fragment of 399bp was amplified from B1 gene, the result showed that 6.74% and 4% of blood samples and 100% and 80% of placental tissue samples which taken from women and sheep respectively were positive to this test.

تحديد القرابة الوراثية لعزلات عراقية ضارية لفيروس مرض النيوكاســــل من تتابع القواعد النتروجينية في موروث الهموكلوتنين نيورامنيديز == Phylogenetic Determination of Newcastle Disease Virus Hn Gene Sequences Iraqi Virulent Isolate

اسم المؤلف: مرتضى عبد المهدي محمد حسن المظفر
اسم المشرف: هدى عبد الهادي علي النصراوي | احمد مجيد حمزة الشمري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض المشتركة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: يعد مرض النيوكاسل من اخطر الامراض الفيروسية المعدية التي تصيب قطعان الدواجن وتسبب خسائر اقتصادية كبيرة في صناعة الدواجن واول تسجيل لمرض النيوكاسل كمرض مشترك بين الانسان والحيوان كان من قبل العالمBurnet في سنة 1943. والهدف من الدراسة هو عزل وتحليل تسل | Newcastle disease is considered to be the most contagious poultry disease and may cause severe economic loss in the poultry industry. The first report in which Newcastle disease virus (NDV) was consider as zoonotic disease and a human pathogen that was published by Burnet, in 1943. The aim of this study was to isolate and determination of nucleotide sequence of the Hemagglutinin - neuraminidase gene (HN) of two NDV Iraqi virulent isolates. These isolates were named as Najaf isolate (Najaf APMV1/ Chicken/ Iraq - Najaf/ ICCMGR/2012) and Baghdad isolate (Baghdad ICCMGR). This work was conducted in Iraq for the first time, the reason behind it was to correlate phylogenetically between these two isolates and the NDV strains in the countries of the region. This will help in determining the source of NDV outbreaks occurred in Iraq. Both Najaf and Baghdad isolates were propagated in embryonated chicken eggs, after inoculation of 11 days embryonated chicken eggs was showed and embryos were killed in different times (more than 40 hours and less than 72 hours). To assess the activity of both virus isolates, the main features of the infected embryos that reflect characteristics of congestion and sever hemorrhage was compared with control one. Hemorrhage was markedly severed in the infected embryos and it was similar in its severity by both isolates. The allantoic fluid was harvested post infection and the agglutination activity reflected titer of 1024 for Najaf isolate and 512 for Baghdad isolate. In order to isolate the Hemagglutinin - neuraminidase gene (HN) from both Najaf and Baghdad NDV isolates, specific primers for this gene were designed. After viral RNA purification, one - step reverse transcriptase - PCR was undertaken to amplify the NH gene and isolated it from the gel. Nucleotide sequence of the isolated NH gene from both virus isolates was determined. The purpose was to focus on molecular and phylogenetic analysis of the hemagglutinin - neuraminidase (HN) gene in both Iraqi isolates. The resulted nucleotide sequence of 647 nucleotides of the HN gene for both isolate were submitted to the GenBank database under the accession numbers KJ632972. To determine the origin of both Iraqi virulent isolates, HN gene sequence of both isolates was aligned with sequences of NDV isolates previously published in GenBank. The resulted alignment was then analyzed in terms of phylogenetic relation and variation. phylogenetic analysis of the isolated virus showed that both Iraqi isolates share significant similarity with 14 other international stains. Two strains of India origin and 12 was China isolates. The identity percentage was 99% - 100% with clustered group. Interestingly, Iraqi isolate was different from the neighbor countries such as Iran, and other Middle East countries. These results may indicate that certain migratory birds might have contributed to the distribution of NDV in Iraq. However imported infected beards from south East Asia may contribute to the current NDV outbreaks caused by foreign strain.

دراسة جزيئية مصلية وكيميائية عن الاصابة بطفيلي انابلازما مارجينالي في الابقار في محافظة الكوت == Some Molecular ,Sero - Prevalence And Biochemical Study of Anaplasma Marginale In Cattle In Al - Kut Province

اسم المؤلف: علا عبد الحسين عكار الكفاري
اسم المشرف: غيداء عباس جاسم الجبوري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: صممت الدراسة للبحث في انتشار مرض الانابلازموسسس بين قطعان الابقار في مناطق مختلفة من محافظة واسط وقد اجري البحث على 184 عينة دم جمعت من ابقارمشكوك في اصابتها تعاني من الحمى (°41c) , فقر دم شديد , شحوب الاغشية المخاطية ,هزال شديد وانخفاض في انتاج الحليب. و| The study was designed to investigate the prevalence of bovine anaplasmosis among cattle from various area in Wassit governorate , the investigation is performed on 184 blood samples collected from suspected cattle suffering from fever (41°C), severe anemia ,pale mucus membrane , progressive emaciation and drop in milk yield , includes (85) males and (99) females , aged < 1year to 2 - 3 years, during October 2012 to April 2013 from AL - Kut , AL - hayy , AL - Bashair , AL - Moufaqia and AL - Noamania areas to investigate antibodies against Anaplasma parasite by using indirect ELISA test and to determine the species Anaplasma marginale by using RFLP - PCR technique, also measuring some biochemical parameters to indicate the effect of the disease on liver function. Results of ELISA test showed that the rate of infection was 13.04 % , and there were different between age groups without significant difference were give 8 % , 11.25 % and 16.45 % in ages < 1year , 1 - 2 year and 2 - 3 years respectively. There was no significant differencerecorded between females that give study revealed that females were give 14.14 % rate of infection and males with 11.7 % rate of infection.The highest rate of infection was recorded in AL - Kut 17.14 % followed by AL - hayy and AL - Bashair were give 14 % ,10 % respectively and the lowest rate was recorded in AL - Noamania and AL - Moufaqia 8.33 % , 5% respectively, the study showed significant differences in incidence of disease between study districts and area in Wassit province under p > 0.05. The most sensitive method used for the diagnosis of anaplasmosis was polymerase chain reaction , DNA extraction was performed only on 24 blood samples which were positive for Anaplasma parasite by ELISA test, the extracted DNA from blood cells were analyzed by PCR and PCR - RFLP technique using primers derived from 16S rRNA gene and restriction endonuclease Bst1107I enzyme which recognized the sequence (GTATAC) in corresponding PCR product of A. marginale and cut it in the position 68, whereas the used restriction enzyme can not cut the corresponding PCR product of other Aanaplasma spp. and the result was 20 from 24 was positive for Aanaplasma spp. by PCR and 18 from 20 was positive for A. marginale. The results of liver enzymes activity showed significant increase in serum AST, ALT, CK and TBIL level in infected cattle (96.8±0.97), (42±0.52) , (406±2.06) and (0.95±0.24) respectively as compared with control (65.5±1.26 ), (21.4±0.45 ) ,( 142±14.17 ) and (0.27±0.05 ) respectively.

دراسة تاثير مستخلص الشاي الاخضر مقارنة بالانسولين على بعض الصفات الفسلجية والتعبير الجيني في الارانب المستحدثة تجريبيا بالسكري == Study The Effect of Green Tea Extract As Compared With Insulin On Some Physiological Parameters And Gene Expression In Experimentally Induced Diabetic Rabbits

اسم المؤلف: ماهر مهدي صالح العارضي
اسم المشرف: حيدر عبد الكاظم نغيش الزاملي
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسه الحاليه لتحديد التاثيرات المحتملة للمستخلص المائي للشاي الاخضر200 ملغم / كغم في اناث الارانب المستحدث فيها السكري تجريبيا. استخدام 35 انثى ارنب بالغة من النوع المحلي تراوحت اعمارها 6 - 10 اشهر معدل اوزانها 1 - 1،5 كغم. قسمت الى خمسة مجاميع مت | The present study aimed to determine the effect of aqueous extract of green tea (200 mg / kg B.W) in experimentally induced diabetic female rabbits.Thirty five female rabbits with arange age of 6 - 10 months and 1 - 1.5 kg weight were used.They are divided into five equal groups.The first group used as negative control (C),The diabetes melltitus (DM) was induced in T1,T2 and T3 by injection of single dose of alloxan 100 mg /kg B.W in marginal ear vein , The T1 assigned as positive control. The T2 treated with 200 mg / kg B.W of aqueous extract of green tea for 8 weeks.The T3 treated with 3 IU/ SC of insulin for 8 weeks while the T4 was given 200 mg / kg B.W of aqueous extract of green tea alone for 8 weeks. After 5 days of diabetes mellitus induction the blood samples were taken from the same vein, the rabbits which have blood glucose more than 200 mg / dl blood consider as diabetic. At the end of the experiment blood samples were taken by cardiac puncture and the blood serum was separated by Centrifuge to measure glucose , albumin, total protein ,liver enzymes and lipid profile and histological sections for pancrease,liver and kidney were done. the Real - time PCR was done to measure the gene expression of (Ins 1) and (Ins 2) which are responsible for insulin hormone production.the results were revealed that there is significant ( p <0.05) decrease in glucose,Low density lipoprotein,very Low density lipoprotein , Triglyceride , Total cholesterol , Alkaline phosphatase , Aspartate Transaminase and Alanine transaminase and significant increase (p <0.05) in Highy density lipoprotein , albumin and total protein in T2 group compared with T1, there is non significant differences in these parameters with T3 group , also there is significant decrease in ( p < 0.05) in Low density lipoprotein,Very Low density lipoprotein , Triglycerid , Total cholesterol and significant increase ( p < 0.05) in Highy density lipoprotein in T4 group compared with all groups. The gene expression results revealed that there is significant ( p < 0.05) increase in Ins 1 and Ins 2 gene expression in T2 compared with T1.The histological study of T1 was explain that there is severe congestion in pancrease with necrosis and absent of islets of langerhans while in the liver there is congestion with large thrombus in central vein with hepatic necrosis. In the kidney there is thrombus and congestion in renal tissue and destruction in renal tubules. While in T2 most damage caused by alloxan was disappeared through regeneration of cells of studied organs

تاثير مستخلص الكركم الخام على التعبير الجيني لمورثة البروتين المنظم للغشاء الحيوي لجرثومة Streptococcus mutans المعزولة من التجويف الفمي == Effect of Curcumine Crude Extract On The Gene Expression of Biofilm Regulatory Protein A (Brp A) In Streptococcus Mutans Isolated From Oral Cavity

اسم المؤلف: نزار احمد شهاب
اسم المشرف: عدنان حمد عبيد الحمداني | عدنان حمد عبيدالحمداني
الموضوع العام: الطب
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: جمعت 100 مسحة فموية من مرضى تسوس الاسنان وامراض ما حول الاسنان الذين تراوحت اعمارهم ما بين 15 - 65 سنة ومن كلا الجنسين والذين راجعوا المركز التخصصي لطب الاسنان في الديوانية للمدة من كانون الاول 2012 الى اذار 2013 لغرض قياس التعبير الجيني لمورثة البروتين ا | A total of 100 oral swabs were collected from patients suffering from dental caries and priodontal infections, ranging from 15 - 65 years old of both genders whom admitted to the Al - Diwaniyah Teaching Special Center of Dentistry within the period from December 2012 to March 2013, in order to quantify the gene expression of biofim regulatory protein (Brp A) in Streptococcus mutans isolates by using the Real Time polymerase chain reaction. According to morphological, cultural characteristics and biochemical tests by using VITEK - 2 system, 34/84(40.47%) isolates of S.mutans were isolated and identified among streptococcus species and the isolation percentages were 26/50(52%) in males and in females were 24/50(48%), while the age group (15 - 25 years old) was the most affected than the other groups. The statistical analysis showed no significant differences among the interaction between gender and age groups. Molecular detection of 16s rRNA gene was amplified to confirm the diagnosis of S.mutans isolates, where all isolates gave an amplicon size (151) bp, in addition to, the DNA sequencing was done in order to identify the nitrogen bases sequences and phylogeny of local isolates in comparison with the global isolates (Spain, Japan, Korea, USA, Canada, India), results revealed that the local isolates of S.mutans had a similarity percent (90%) with Spain isolates.Furthermore, the susceptibility of isolates to a variety of antibiotics have been investigated. It has been found that isolates had multiresistance to erythromycin(88.2%),ampicillin(76.4%),amoxiclave(70.5%),tetracycline(61.7%), nalidixic acid and amoxicillin (58.8%), chloramphenicol (55.8%). The statistical analysis showed a significant differences among tested antibiotics. Biofilm formation was investigated phenotypically by using tissue culture plate with crystal violet staining for resistant S.mutans isolates, the results revealed that all isolates were high biofilm former, furthermore, the effect of ethanolic extraction (50%) of curcumine in growth inhibition and biofilm reduction of S. mutans was assayed. The results showed an effect of curcumine in comparison with chlorhexidine(2%) as a control.The gene expression of biofilm regulatory protein A(Brp A) was quantified in comparison with housekeeping gene 16s rRNA as a reference gene of biofilm S.mutans by using reverse transcriptase polymerase chain reaction (RT - PCR). The results revealed that the expression (relative&absolute) of Brp A gene is decreased in the test treatment (S.mutans with 1% glucose+ 50% curcumine) in comparison with control (S.mutans grown in 1% glucose only).In conclusion, the present study established the role of Brp A gene in biofilm formation

حول بعض الفضاءات التبولوجية باستخدام المجموعة المفتوحة == On Some Topological Spaces By Using b - Open Set

اسم المؤلف: علي سامي ماجد الزاملي
اسم المشرف: رعد عزيز حسين العبد الله
الموضوع العام: علوم الرياضيات
السنة: 2011
الموضوع الدقيق: التبولوجيا
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

On Some Geometrical Aspects In Geometric Function Theory

اسم المؤلف: فائز جواد عبد الكاظم
اسم المشرف: Waggas Galib Atshan
الموضوع العام: علوم الرياضيات
السنة: 2014
الموضوع الدقيق: الرياضيات
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

دراسة نموذج التلوث مع التطبيق حول نهر الديوانية == Study Of A Pollution Model With Application On Al - Diwaniya River

اسم المؤلف: زينب محمد نجم
اسم المشرف: سليم عزارة حسين | وقاص غالب عطشان
الموضوع العام: علوم الرياضيات
السنة: 2012
الموضوع الدقيق: الرياضيات
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

بعض المسائل المتعلقة بالفضاء المتري الضبابي == Some Problems Related To Fuzzy Metric Space

اسم المؤلف: انتصار حربي راضي
اسم المشرف: نوري فرحان عذاب المياحي
الموضوع العام: علوم الرياضيات
السنة: 2012
الموضوع الدقيق: الرياضيات
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

القدرة التمنيعية لمتعدد السكريد الشحمي المستخلص من بعض البكتيريا الممرضة في الجرذان البيض == The Immunogenicity Of Lipopolysaccharide Extracted From A Certain Pathogenic Bacteria In White Rats

اسم المؤلف: ليث ابراهيم عليوي الشباني
اسم المشرف: زياد متعب الخزاعي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

التحري عن 2-Methylisoborneol و Geosmin المنتج من بعض انواع الطحالب الخضر المزرقة تحت ظروف مختلفة == Investigation Of Geosmin And 2 - Methylisoborneol Produced By Some Species Of Cyanophyta Under Different Conditions

اسم المؤلف: حیدر عبد الواحد مالك الغانمي
اسم المشرف: فواد منحر علكم | میسون مھدي الطائي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة المائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

Sub Clinical Lead Toxicity And It'S Correlation With Some Hormones And Blood Parameters Of Adult Men In Al - Diwania Governorate

اسم المؤلف: زينب ابراهيم محمد
الموضوع العام: علوم الحياة
السنة: 2006
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

دراسة بكتريولوجية لجرثومة ايشيريشيا القولون المرضية المعوية في دجاج اللحم في محافظة الديوانية == Bacteriological Study Of Enteropathogenic E.Coli Isolates From Broilers In Aldiwaniya Governorate

اسم المؤلف: جبار عفات علوان الوائلي
اسم المشرف: عدنان مهران اوانيس
الموضوع العام: الطب البيطري
السنة: 2007
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

اصابات الكلاميديا في الطيور والانسان == Avian Chlamydiosis in Birds and Human

اسم المؤلف: عقيل مسلم نعمة
اسم المشرف: علاء عبد العزيز عبد
الموضوع العام: الطب البيطري
السنة: 2012
الموضوع الدقيق: الامراض - الدواجن
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

دراسة وبائية عن داء الجيارديا في الانسان والابقار == An Epidemiological Study About Gairdiasis In Humans And Cows

اسم المؤلف: شيماء عبد جبر الزيادي
اسم المشرف: نعمان ناجي عايز
الموضوع العام: الطب البيطري
السنة: 2012
الموضوع الدقيق: الامراض المشتركة
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

دراسة تاثير الشاي الاخضر في وظيفة عضلة القلب للارانب المعاملة بالنترات == Study Of The Effect Of Green Tea On Cardiac Muscle Function In Nitrate Treated Rabbits

اسم المؤلف: حيدر غازي عبد الشهيد الفتلاوي
اسم المشرف: حيدر عبد الكاظم نغيش الزاملي
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
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