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تعقب الليشمانيا الجلدية بالاختبارات التحليلية الوبائية والطفيلية والجزيئية والكيمياحياتية == Tracking of Cutaneous Leishmaniasis By Epidemiological, Parasitological, Molecular And Biochemical Analysis

Author name: سندس نصیف الحجیمي
Supervisor name: باقر عبیس سلطان | محسن عبد الحسین الظالمي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: The present study was performed to identify the species and strain of Leishmania parasite isolated from different endemic areas. It was carried out on cutaneous leishmaniasis (CL) in five Iraqi provinces as following : Al - Najaf province ( Al - Hakeem and Al - Sadder teaching hospitals), Babylon province ( Marjan hospital ), Al - Qadisya province (AL - Sadder Teaching hospital ), Karbala province (Al - Hussain General Hospital) and Kut province (Al - Zehraa Teaching and Al - Kerama Hospitals). It covered seven hospitals as a field of investigations from October 2010 to December 2012.Cellulose acetate electrophoresis has been performed in Walter Reed Institute of researches in USA. A total of 126 cases comprising 48(38%) females and 78(62%) males were studied. The highest infected age group was 21 - 30 years with a rate of 27.8% and the lowest rate was 11.9% at the age group of 10 and less years.Geographical distribution of CL among hospital patients indicated that rural areas were with highest rate (54%) while in urban areas were with(46%).The number of ulcers per one patient differs for each patient. The highest rate of multiple lesions was 73% in comparison with single lesion (27%).The type of infection showed that disease was in wet type (82.5%) more than in dry type (17.5%). Direct smears were made by lesion aspiration,skin scraping and discharged blood methods , by which 90 (71%) patients gave positive result under light microscope.Modified NNN medium and RPMI - 1640 with fetal bovine serum followed by sub - culture in drosophila Schniders media were performed for each case. Out of 126 cases,83(65%) gave positive growth. Further characterization of the causative parasite species and strain made by molecular and biochemical techniques.Out of 83 postive growth culture,only 52 specimens were studied by nested - PCR, using kinetoplast minicircle fraction amplification to detecte the species of parasite.It was found that 45(86.5%) cases in the generation of a 560 bp DNA and 7(13.4%)patients displayed a fragment of 750 bp, corresponding to L. major and L. tropica, respectively.This test revealed that L. tropica and L. major are the causative agents of infection with dominant shifting to the L. major.Cellulose acetate electrophoresis (CAE) was performed for 20 mass cultivated cultures. Isoenzyme profiles of these isolates were compared with reference strains of Leishmania spp. using cellulose acetate electrophoresis and 4 enzyme systems (glucose phosphateisomerase,leucil phosphate,manose phosphate isomerase and 6phosphogluconate dehydrogenase). The results showed that L.major(LV39) isolate were in 20 mass cultivated culture.Most of the CL patients in Najaf province(2011,2012) were from Haidariya in north district. High prevalence of disease was observed in Autumn and Winter.The highest number of cases was recorded during February and December.

مقارنة بين النوع الثاني من مرض السكري ومرض السكري المناعي الذاتي المتاخر الظهور للبالغين باستخدام مؤشرات الخلايا اللمفاوية وملف الحركيات الخلوية == Comparison Between Type 2 Diabetes Mellitus And Latent Autoimmune Diabetes of Adults Using Lymphocytes Markers And Cytokines Profile

Author name: سوسن محمد جبار الحسناوي
Supervisor name: سلمان عزيز الجبوري
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: This study was done to investigate whether Type 2 diabetes and Latent autoimmune diabetes of adult (LADA) patients have similar or different immunological profile.Therefore, measurement of serum levels of adhesion molecules (Serum intracellular cell adhesion molecule(sICAM - 1), Serum vascular cell adhesion molecule (sVCAM - 1) and Serum endothelial Selectin (sESelectin)), chemokines (CCL2, CCL3 and CCL4) and cytokines (IL - 6, IL - 1receptor antagonist and IFN - ?) in these patients was done. In addition to the influence of BMI, gender, age and diabetes duration on these systemic immune mediators were analyzed. Also, the relationships of the in vivo levelsof these mediators with micro - & macro - vascular complications in type 2 diabetes & LADA were studied.Three hundred individual were enrolled in this study and were divided into two subgroups : patients and control groups (280 patients& 20 control). From the 280 patients group who were clinically diagnosed as type 2 D.M., 34 patients were GADA positive and considered as LADA cases. Serum samples were taken from The 34 LADA cases, 36 cases of D.M.2 (selected randomly from the remaining 246 diabetic type 2 patients) & 20 healthy control subjects.The prevalence of LADA among D.M.2 patients was 12.1%. A significant difference in family history, BMI, FBS, HA1C & duration of D.M. was found in D.M.2 cases compared to LADA cases. The mean sVCAM - 1level of control group was significantly lower than D.M.2 and LADA group, P<0.05. The sE - Selectin level was higher in LADA group than control and D.M. type 2 group & significantly different when compared to control group (P<0.05). The sVCAM - 1 level had a significant positive correlation (R=0.25, P=0.017) with BMI in all groups. LADA group had significantly higher values of CCL2 while D.M. type 2 had the least values (P=0.002). The mean IL - 6 of D.M. type 2 group was (11.6±3.1), for LADA group it was (12.4±1.7) and for the controls it was 0.95 ± 0.14. There was a statically significant difference between D.M.2 vs. controls, and LADA vs. controls (P<0.05), regarding the mean IFN - ?, it was significantly higher in LADA group than D.M.2 group (87.5 ± 20.3) vs. (40.3 ± 4.5) respectively, (P=0.032).The mean IL - 1receptor antagonist was significantly higher in D.M. type 2 and LADA cases than controls (P<0.05). A significant positivecorrelation between IL - 6 and sICAM - 1, (R= 0.27, P= 0.013) & a highly significant positive correlation between IL - 6 and IL - 1RA, (R= 0.35, P=0.001) were found. IL - 6 & IL - 1RA serum levels in D.M. type 2 patientswere significantly associated with the complications where p - value?0.05. For conclusions, LADA is clinically underestimated among D.M. type 2 patients and increases with increasing age. Also, Levels of CCL2 and IFN - ? are associated with autoimmunity, Levels of adhesion molecules & chemokines are associated with complications of diabetes.

تاثير الايتانرسبت على السايتوكينات وموت الخلايا المبرمج جراء انقاص الارواء الدموي واعادته لعضلة القلب في ذكور الفاران == Effect of Etanercept Against Myocardial Ischemia/Reperfusion Injury In Male Mice

Author name: سيف محمد حسن
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: يمثل نقص ارواءعضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. تشمل اصابة عضلة القلب بسبب نقص التروية الدمويه واعادتها ضعف مقلص القلب، عدم انتظام ضربات القلب وكذلك تلف الخلايا العضلية التي لا رجعة في | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem associated with thrombolysis, angioplasty and coronary bypass surgery. Injury of myocardium due to ischemia - reperfusion includes cardiac contractile dysfunction, arrhythmias as well as irreversible myocytes damage. These changes are considered to be the consequence of imbalance between the formation of oxidants and the availability of endogenous antioxidants in the heart. Objective : This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model.Material & method : adult male Albino mice were randomized into four equal groups.1. Group (1) : Sham group : mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery.2. Group (2) : Control group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours.3. Group (3) : Control vehicle group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice received vehicle of etanercept (normal saline) 5 minutes before reperfusion via I.P injection and.4. Group( 4) : Etanercept treated group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice treated with etanercept 5mg/kg i.p 5 minutes before reperfusion XVIIIResults : Compared with the sham group, the levels of TNF - ? & IL - 1?, IL - 6, Caspase 3 and plasma level of cardiac troponin I increased in the control group but decreased level of Bcl - 2 (p<0.01).Histologically, all mice in the control group showed significant (p<0.01) cardiac injury and apoptosis.Etanercept significantly decreased in myocardium level of TNF - ?, IL - 1B, IL - 6,Caspase 3 , and plasma cTnI (P < 0.01), while significantly increased level of Bcl - 2 (P < 0.01). Histological analysis revealed that etanercept markedly reduced (P < 0.01) the severity of cardiac injury in the mice underwent LAD ligation procedure. : ionsConcluThe results of the present study reveal that etanercept may ameliorate myocardial I/R injury in mice via interfering with inflammatory reactions and apoptosis. : RecommendationAfter studying the results of the present study, the following recommendation to further1. Further measuring the P - selctine and E - selectine to show the effect of etanercept on rolling of eutrophils and platelets that cause further occlusion of blood vessels.2. Further measure the adiponectine (that have a cardioprotective effect ).

العلاقة بين اعتلال الجهاز العصبي اللاارادي الناتج عن السكري مع مستوى اللبتين وامراض القلب حسب فحص ايكو القلب وفحص اجهاد القلب == Relation of Diabetic Autonomic Neuropathy With Leptin And Cardiac Disease Assessed By Echo And Treadmill Test

Author name: زينب فلاح حسن
Supervisor name: احسان محمد عبود عجينة | علاء حسين عباس
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: الخلفية : الاعتلال العصبي اللاارادي القلبي له تاثير سلبي على الحياة ونوعيتها للاشخاص المصابين بالسكري. الاعتلال العصبي اللاارادي القلبي يعتبر نوع فرعي من الاعتلال العصبي السكري. هذا النوع من الاعتلال هو الاكثر بروزا لتهديده للحياة وبسبب توفر الفحص القلبي | Background : Cardiac autonomic neuropathy (CAN) has a negative effect on survival and quality of life in people with diabetes mellitus (DM) and is regarded as a subtype of the diabetic autonomic neuropathy. This type of neuropathy is the most prominent focusing because of its life threatening and the availability of the cardiovascular tests that can diagnose it. It has been thought that many factors in DM like leptin causing increase the risk of cardiovascular disease. Researches about the relation of leptin with CAN and cardiovascular disease are yet limited.Aim of study : This study aims to determine the prevalence of CAN in patients with type 2 diabetes mellitus, evaluate the association between CAN the presence of left ventricular dysfunction in these patients, test the association between CAN and ischemic heart disease and assess the relationship between the presence and severity of CAN with fasting serum leptin in these patients.Patients, materials and methods : This cross sectional study included 103 (56 males and 47 females) diabetic patients without hypertension or pre - diagnosis of ischemia. They had attended to the diabetic center in Marjan Medical City in Hilla from March 2013 to February 2014. The patients had undergone thorough assessments that included clinical (history and full examination) and biochemical assessments (measurement of fasting plasma sugar, glycated haemoglobin, lipid profile, blood urea, serum creatinine and serum leptin level). Cardiac autonomic reflex tests were also assessed along with echocardiography and treadmill test. Cardiac autonomic reflex tests included the five tests of Ewing protocol. Patients who had two or more abnormal tests were considered as having CAN (75 patients) and those with less than two abnormal tests were considered as patients without CAN (28 patients). Results : The study found that most diabetic patients (84%) were obese (BMI?25 kg/m2) and most of the diabetic patients presented with CAN (72.8%). Fasting serum leptin level was higher in female than in male in both study groups (with and without CAN). Patients with CAN were older when compared to patients without CAN (p?0.01) and had longer duration of DM (p?0.01) with significantly higher fasting serum leptin levels. The leptin level showed a tendency to increase as the score of CAN increased. However, these changes were not significant (P>0.05). The most abnormal sympathetic response was diastolic blood pressure response to hand grip while the most abnormal parasympathetic response was heart rate response to breathing. On contrary, the lowest abnormal response was found in postural blood pressure test. Echocardiography showed that most of the diabetic patients (89.8%) had a left ventricular hypertrophy by measuring the left ventricular mass index. However, there were no significant changes in echocardiographic results between patients with CAN and those without.Conclusions : This study concludes that CAN is a common complication of type 2 diabetes that affected a large percentage of diabetic patients. The duration of the diabetes and the age of patients are important non modifiable risk factors for the development of CAN. Additionally, heart rate variability is considered as an important test for early detection of CAN. On the other hand, CAN has no impact on the occurrence of left ventricular diastolic dysfunction. Leptin level was high in diabetic patients and even higher in those with CAN and its levels related positively with the severity of CAN. Also, there were high leptin levels in diabetic patients with positive treadmill test results, although these changes were not significant (P>0.05).

تقييم مستضد البروستات النوعي في مصل مرضى التهاب المسالك البولية == Evaluation Of Prostate Specific Antigen In Urinary Tract Infection Patients Serum

Author name: علي جبار عبد زيد الكلابي
Supervisor name: وسن سامي حميد
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Najaf
First pages:

مؤشرات الاورام (المستضد السرطاني الجنيني والبروتين الجنيني الالفي) في سرطان الثدي والجهاز الهضمي == Tumor Markers (CEA,and AFP) In Breast And Gastrointestinal Tract Cancers

Author name: حيدر عبد القادر الجوادي
Supervisor name: اكرم جرجيس الباجةجي
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
Language: English
University location: Najaf
First pages:

تقييم حالة علامات الالتهاب لدى مرضى السكري من النوع الثاني مقارنة بمرضى اعتلال الكلى السكري == Evaluation Of Inflammatory Status In Patients With Type 2 Diabetes Mellitus Versus Diabetic Nephropathy

Author name: رائد عبد علي ميران شريف
Supervisor name: رائد عبد علي ميران شريف
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
Language: English
University location: Najaf
First pages:

ترافق كالسيوم الشريان الابهر البطني مع كالسيوم الشرايين التاجية ومرض الانسداد التاجي == Association Of Abdominal Aortic Calcium With Coronary Artery Calcium And Obstructive Coronary Artery Disease

Author name: حنان محمد علي الرماح
Supervisor name: حيدر قاسم الموسوي | فاضل غالي العمران
General topic: Medicine
Specific topic: Diagnostic Radiology
Degree: Higher Diploma
Language: English
University location: Najaf
First pages:

التناظر الجيني لموروثة الاديبونكتين وارتباطه مع الداء السكري ومقاومة الانسولين == Adiponectin Gene Polymorphisms (SNPs - 11391G>A and - 11377C>G) Association With The Occurrence Of Type 2 Diabetes Mellitus and Insulin Resistance

Author name: انتظار صاحب محمد
Supervisor name: ماجد كاظم حسين
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
Language: English
University location: Najaf
First pages:

تاثير السمفاستاتينوالاربيزارتان على الكيموكينات والسايتوكينات وموت الخلايا المبرمج جراء انقاص الارواء الدموي واعادته لعضلة القلب في ذكور الجرذان == Effect Of Simvastatin & Irbesartan On Chemokines, Cytokines And Apoptosis In Myocardial Ischemia/Reperfusion Injury In Male Rats

Author name: سهاد تريجي زامل العكيلي
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:

تاثير الـ - ميثيونين والـ - كارنيتين في الضرر الموضعي الناتج من نقص التروية / اعادة التروية وموت الخلايا المبرمج في ذكور الجرذان == The Effect Of L - Methionine And L - Carnitine In Regional Ischemia - Reperfusion Injury And Apoptosis In Male Rats

Author name: فرقان هاشم حسين
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:

تاثير الاملوديبين ? كانديسارتان ومزيجهما في الاصابة بنقص التروية الدماغية واعادة الارواء في الجرذان == Effect Of Amlodipine, Candesartan And Their Combination In Rat Cerebral Ischemia Reperfusion I/R Injury

Author name: زهراء كاظم الحسني
Supervisor name: نجاح رايش هادي الموسوي | وضاح محبوبة
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:

العلاقة بين الفسفاتين والرسستين معد عدم الاستجابة للانسولين اثناء الحمل == Relevance Of Visfatin And Resistin Level Changes With Insulin Resistance In Pregnancy

Author name: اسيا علي حمزة
General topic: Medicine
Specific topic: Clinical Biochemistry
Degree: Master
Language: English
University location: Najaf
First pages:

ظهور ZAP 70 في خزعة نخاع العظم لمرضى ابيضاض الدم اللمفاوي المزمن في العراق : دراسة مناعية نسيجية كيميائية == ZAP 70 Expression In Bone Marrow Biopsy Of Chronic Lymphocytic Leukemia (CLL) Among Iraqi People Immunohistochemical Approach

Author name: محمد صادق مهدي الموسوي
Supervisor name: قسور موسى الطريحي | رحيم مهدي
General topic: Medicine
Specific topic: Diseases - Blood
Degree: Master
Language: English
University location: Najaf
First pages:

تاثيرات فيتامين ج على بعض معايير الدم وايض الحديد خلال الدورة الشهرية لدى نساء مقاطعة النجف الاشرف == The Effect Of Vitamin C On Some Blood Parameters And Iron Status During Menstrual Cycle In Women Of Holly Al - Nagaf Al - Ashraf Province

Author name: نغم يحيى غافل سعيد
Supervisor name: سامي رحيم الكاتب
General topic: Medicine
Specific topic: Physiology
Degree: Master
Language: English
University location: Najaf
First pages:

مدى حدوث اصابة عنق الرحم بالفيروسات الحليمية البشرية ذات الاختطار العالي والتنميط الجزيئي لتلك الفيروسات == Occurrence And Molecular Detection Of High Risk Cervical Human Papillomaviruses Infection

Author name: منال عبد علي فرحان الحميداوي
Supervisor name: سلمان عزيز الجبوري | ازهار موسى الطريحي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Najaf
First pages:

دراسة مايكروبايولوجية لمرض الداحوس المزمن عند ربات البيوت : عزل، تشخيص والمعالجة ا لفعالة == A Microbiological Study Of Chronic Paronychia In Housewives : Isolation, Identification And Effective Treatment

Author name: كريمة امين حسين الخفاجي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Najaf
First pages:

دراسة مناعية نسيجية لسرطان الثدي لمرضى الاعمار المبكرة بالمقارنة مع الاعمار المتوسطة بتطبيق عاملي BRCA1 وp53 في النجف == Immunohistochemical Study Of Breast Carcinoma In Early Age Patients As Compared With Middle Age By Application Of BRCA1 And P53 In Al - Najaf

Author name: فاطمة مطشر البديري
Supervisor name: صباح احمد عبد ابو صيبع | عقيل عبد ياسين
General topic: Medicine
Specific topic: Tissue Diseases
Degree: Master
Language: English
University location: Najaf
First pages:

دراسة تاثير الثوم، دهن السمك، الادرينوكورتكوتروفين والدكساميثازون على مؤشرات التخثر ومستوى الدهون في مصل الدم لدى ذكور الجرذان المصابة ارتفاع مستوى الدهون

Author name: علاء غازي حميد العبودي
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:

دراسة التركيب الوراثي الجزيئي والمناعي على مرضى الذين يعانون من فيسروس التهاب الكبد نمط B في الفرات الاوسط من العراق == Molecular Genotypes And Immunological Study On Patients With Hepatitis B Virus In Middle Euphrates of Iraq

Author name: عباس عطية شعلان سراق
Supervisor name: غانم عبود جابر المولى | سلمان عزيز عدوس
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Najaf
First pages:

الدور المناعي للمعلمات السرطانية في تشخيص سرطان القولون والمستقيم == Immunological Role Of Tumor Markers In Diagnosis Of Colorectal Cancer

Author name: ضمياء يوسف محمد سعيد الحارس
Supervisor name: جعفر كاظم نعمة الموسوي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Najaf
First pages:

التحري عن الفايروس البشري المضخم للخلايا في حالات الاجهاض التلقائي والعقم == Detection Of Human Cytomegalovirus Infections In Spontaneous Miscarriage And Infertility

Author name: سيف جبار ياسر
Supervisor name: كريم ثامر مشكور الكعبي | غانم عبود المولى
General topic: Medicine
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Najaf
First pages:

دراسة تاثير الالسكايرن والافبرادين وكليهما معا على تقدم اعتلال الكلى السكري == Effect Of Aliskiren And Ivabradine And Their Low Dose Combination On The Progression Of Diabetic Nephropathy

Author name: زهراء عبد الكريم
Supervisor name: باسم ارحيم محمد الشيباني
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:

مفاهيم تشخيصية لتكوين الاجسام المضادة الذاتية جراء الاصابة بمرض الاكياس المائية في الانسان == Diagnostic Aspects Of Autoantibodies Production In Human Hydatid Disease

Author name: خالد رؤوف كریم
Supervisor name: باقر عبیس سلطان | صباح نعمة مح
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Master
Language: English
University location: Najaf
First pages:

الخصائص الجزيئية للمبيضات البيضاء المعزولة من المرضى المثبطين مناعيا في النجف == Molecular Characterization Of Candida Albicans Isolated From Immunocompromised Patients In Najaf

Author name: حيدر محمود علي سماكة
Supervisor name: عدنان حمد عبيد الحمداني | علي محسن المحنة
General topic: Medicine
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: English
University location: Najaf
First pages:
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