تاثير الايتانرسبت على السايتوكينات وموت الخلايا المبرمج جراء انقاص الارواء الدموي واعادته لعضلة القلب في ذكور الفاران == Effect of Etanercept Against Myocardial Ischemia/Reperfusion Injury In Male Mice
Author name:
سيف محمد حسن
Supervisor name:
نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic:
Medicine
Specific topic:
Medicines and Toxins
Degree:
Master
University:
University of Kufa - Faculty Of Medicine - Department Of Medicine And Therapeutics
Language:
English
University location:
Najaf
First pages:
19T1100 - p.pdf
Abstract:
يمثل نقص ارواءعضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. تشمل اصابة عضلة القلب بسبب نقص التروية الدمويه واعادتها ضعف مقلص القلب، عدم انتظام ضربات القلب وكذلك تلف الخلايا العضلية التي لا رجعة في | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem associated with thrombolysis, angioplasty and coronary bypass surgery. Injury of myocardium due to ischemia - reperfusion includes cardiac contractile dysfunction, arrhythmias as well as irreversible myocytes damage. These changes are considered to be the consequence of imbalance between the formation of oxidants and the availability of endogenous antioxidants in the heart. Objective : This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model.Material & method : adult male Albino mice were randomized into four equal groups.1. Group (1) : Sham group : mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery.2. Group (2) : Control group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours.3. Group (3) : Control vehicle group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice received vehicle of etanercept (normal saline) 5 minutes before reperfusion via I.P injection and.4. Group( 4) : Etanercept treated group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice treated with etanercept 5mg/kg i.p 5 minutes before reperfusion XVIIIResults : Compared with the sham group, the levels of TNF - ? & IL - 1?, IL - 6, Caspase 3 and plasma level of cardiac troponin I increased in the control group but decreased level of Bcl - 2 (p<0.01).Histologically, all mice in the control group showed significant (p<0.01) cardiac injury and apoptosis.Etanercept significantly decreased in myocardium level of TNF - ?, IL - 1B, IL - 6,Caspase 3 , and plasma cTnI (P < 0.01), while significantly increased level of Bcl - 2 (P < 0.01). Histological analysis revealed that etanercept markedly reduced (P < 0.01) the severity of cardiac injury in the mice underwent LAD ligation procedure. : ionsConcluThe results of the present study reveal that etanercept may ameliorate myocardial I/R injury in mice via interfering with inflammatory reactions and apoptosis. : RecommendationAfter studying the results of the present study, the following recommendation to further1. Further measuring the P - selctine and E - selectine to show the effect of etanercept on rolling of eutrophils and platelets that cause further occlusion of blood vessels.2. Further measure the adiponectine (that have a cardioprotective effect ).
