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الاصابة ببكتريا Helicobacter pylori وعلاقتها بمرض تصلب الشرايين القلبية == Correlation Between Helicobacter Pylori Infection And Atherosclerotic Heart Disease

Author name: رواء سعدي سلمان
Supervisor name: مي خليل اسماعيل
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: مرض قصور الشرايين التاجية هو النتيجة النهائية لتراكم اللويحات العصيدية ضمن جدران الشرايين التاجية مسببة نقص الاوكسجين وبالتالي حدوث مرض القلب الاقفاري. ويعتبر من الامراض الشائعة واحد الاسباب الرئيسية لحالات الوفاة في العالم. اضافة الى عوامل الخطورة التقلي | Coronary artery disease (CAD) is the end result of the accumulation of atheromatous plaque within the walls of the coronary arteries resulting in shortage of oxygen supply and ischemic heart disease (IHD). It was consider as one of the most common diseases and major causes of worldwide morbidity and mortality. In addition to traditional risk factors for cardiovascular disease, nowadays, accumulating evidence indicates that a variety of infectious agents may contribute to pathogenesis of CAD. The present study evaluated the anti - Helicobacter pylori IgG and IgA and the role of virulence factor of H. pylori cytotoxin associated gene (Cag A) and vacuolating associated cytotoxin (Vac A) as a risk factors for CAD.Eighty patients were divided into 2 groups : first group was 70 patients with CAD, the other group contained 10 patients with another coronary artery disease; they were admitted to Ibn Al - Bitar Specialist Center for Cardiac Surgery in Baghdad between October 2013 and January 2014. Ten individuals used as a healthy control group. All blood samples were tested biochemically such as glucose test, urea test, creatinine test and lipid profile test(cholesterol, triglyceride, LDL and HDL) and all of them showed normal results. The present study revealed that males were affected more than females (3 : 1); with no relation between their ages and bacterial infection.Serum IgG and IgA was Estimated by indirect immunofluorescent (IIF) whereas Cag A, Vac A and high sensitive C - reactive protein (hs - CRP) measured by enzyme linked immunosorbent assay (ELISA) and compared to control group results.The incidence of the anti - H.pylori IgG was highly significantly prevalent in CAD patients 78.57% (55/70), than in control group 0% (0/10), also a high significant difference in another CAD patient group 100%(10/10) as compared to control group 0% (0/10) (P? 0.01).Similarly anti - H. pylori IgA in CAD patients and another CAD patient groups showed highly significant increase when compared to control group 37.14% (26/70), 30% (3/10) and 0% (0/10) respectively (P? 0.01).The incidence of the CagA - positivity was significantly prevalent in mean value (2.74±0.19) in patients with CAD and another CAD patient group(2.72±0.31) than in control group (1.64± 0.16) (P? 0.05). Same result was observed with Vac A antigen; mean value of patient group showed significant increase (1.299 ± 0.04) when compared to controls group (1.41±0.13) (P? 0.05). Also significant increase found between another CAD patient group(1.79±0.17) and control group (1.41±0.13) (P? 0.05). Furthermore; the present study revealed significant differences of concentration levels between hs - CRP in CAD patient group (4.95 ± 0.38 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml), as well as a significant differences found between another CAD patient group (3.96 ± 0.96 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml) (P? 0.05).

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

Author name: محمد خضير عباس النعيمي
Supervisor name: عباس عبود فرحان الدليمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.

تحديد الصفات المظهرية والوراثية لانواع بكتريا المكورات المعوية المعزولة من المرضى في محافظة النجف الاشرف == Phenotypic And Genotypic Detection of Enterococcus Sp. Isolated From Patients In Al - Najaf Al - Ashraf Governorate

Author name: زهراء حميد عودة القريشي
Supervisor name: مهدي حسين محيل العمار
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: The study aimed to isolate and identify the Enterococcus spp. from different clinical specimens and study the virulence factors of predominant species, as well as detected the virulence factors encoding genes such as efaA (endocarditis - associated antigen), esp (enterococcal surface protein), eep (stimulating of pheromones expression) and enlA (enterolysin A) genes by PCR techniques.There were three hundred clinical specimens collected from patients suffering from different clinical infections during the period from September 2013 to January 2014 in AL - Sadder Medical City and AL - Hakem General Hospital. The identification of the Enterococcus spp. isolates were depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was performed with the automated VITEK - 2 compact system using Gram positive - Identification (GP - ID) cards.According to the results obtained by the VITEK tests forty two clinical isolates of Enterococcus were detected, which distributed into : (22) isolates from urine, (8) vaginal swabs, (6) seminal fluid, (4) throat swabs and two isolates from wound swabs with no isolates from cerebral spinal fluid, stool and blood specimens.This study revealed that the E. faecalis is more distributed with 25(59.52%), followed by E. faecium with 10 (23.80%), E.avium with 5 (11.90 %), E. durans and E. raffinosus with 1(2.39%) for each.The study investigated the virulence factors of E. faecalis, E.faecium and E.avium, which play a major role in enterococcus pathogenicity. E. faecalis and E.faecium had the ability to producecapsule, gelatinase, biofilm, adhesion, protease, bacteriocin, haemolysin and cytolysin except ? - lactamase produced only by E. faecalis while E.avium produced all these virulence factors except gelatinase, bacteriocin and cytolysin.The results revealed variation in the resistance of bacteria to antibiotics, E.faecalis express absolute resistance (100%) to Erythromycin, high resistance against Gentamycin, Tetracyclin and Vancomycin but high susceptibility to Ciproflaxacin and Penicillin and moderate susceptibility to Chloramphenicol. E.faecium exhibited absolute resistance (100%) to Erythromycin, Gentamycin and Tetracyclin, high resistance against Chloramphenicol, Penicillin and Vancomycin but high susceptibility to Ciproflaxacin while E.avium exhibited susceptibility (100%) to all these antibiotics except Tetracyclin showed absolute resistance.Then detected the virulence factors encoding genes : efaA, esp, eep and enlA genes by using PCR techniques and Electrophoresis Systems.Finally, The genotypic method. Regard to genotypic study the outcome showed that 21(84%) isolates of E.faecalis, 4(40%) of E.faecium and 5(100%) of E.avium were carrying efaA gene and 17(68%) of E.faecalis, 5(50%) of E.faecium, 5(100%) of E.avium were carrying esp gene while eep gene was carrying only by E.faecalis and E.faecium ; 12(48%) and 3(30%) respectively. Also the results revealed that only 1(4%) isolates of E.faecalis have enlA gene.

العلاقة بين مرض السكري المناعي الذاتي الكامن النمط الثاني للبالغين مع الخمج بالملوية البوابية == Association Between Latent Autoimmune Diabetes Of Adults In Type Ii Dm With Helicobacter Pylori Infection

Author name: سيف علي مكلف
Supervisor name: عروبة خالد عباس | مدين علي حسين
General topic: Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: Latent Autoimmune Diabetes in Adults (LADA) is a form of diabetes which is less recognized and underdiagnosed type of diabetes which appears to have characteristics of both type 1 (autoimmune in nature) and type 2 diabetes (adult age at onset).Helicobacter pylori (H. pylori) infection has a worldwide distribution. The bacteria colonize more than half of the world population and consider the main cause of gastritis, peptic ulceration, gastric adenocarcinoma and gastric lymphoma. The host immune response is unable to clear the infection and may actually contribute to the associated pathogenesis, also the bacterial virulent factors had certain role in the pathogenicity of infection as it was stated by limited studies that the Cytotoxin associated gene A (CagA) protein and Vacuolating cytotoxin gene A (VacA) protein positive strains are possibly associated with severe infections.The present study was designed to find out the percent of LADA patients among group of type II Iraqi diabetic patients and the relation between LADA patients and H. pylori infection.The study was carried out on 350 type II patients attending the National Diabetes Center/ Al - Mustansirrhea University/ Baghdad Al - Yarmouk during the period May to October 2014 were recruited for this study. Diagnostic markers for LADA, namely, C - peptide levels by Radioimmunoassay and GAD autoantibodies by ELISA test were determined and other Various parameters such as, HbA1c levels, Anti - H.pylori IgG, Anti - CagA protein and Anti - VacA protein by ELISA test were evaluated and correlated with type 2 diabetes and 40 control, matched by age and gender were also included.57 patients of 350 of type II have low C - peptide level, from which, 40 patients have Anti - GAD65 positive and 17 patients have Anti - GAD65 negative.The percentage of LADA patients among type II diabetes was 11.4%.The mean serum levels of C - peptide of LADA patients, controls and type II diabetes (0.30 vs. 1.94 and 3.15ng/ml) were significantly lower in LADA patients as compared to controls and type II patients (p?0.05).Also the mean blood concentrations of HbA1c of LADA patients, type II and controls (8.5 vs. 7.5 and 5.2 %) were significantly higher in LADA patients as compared to type II patients and controls (p?0.05).The Higher frequency of anti - H. pylori IgG was in LADA patients when compared with patient's controls and healthy controls (59.6%, 21.3% and 19.1%) and the p - value (?0.05).In conclusion, Cytotoxic associated gene A protein (Cag A) protein is more frequency with H. pylori positive patients 36/47 (76.6%) indicate an important role of this virulence factor in the pathogenesis of H. pylori, so they could be considered as a good biomarker for discern the risk of developing serious gastroduodenal disease in the host, than Vacuolating cytotoxin A Protein (Vac A) which is less frequency than Cag A in H. pylori positive patients 16/47 (34%).

التحري الجزيئي للجزر الوراثية المسؤولة عن المقاومة للمضادات الحياتية في العزلة baumannii A92 Acinetobacter == Molecular Detection Of Genomic Islands Responsible For Antimicrobial Resistance In Acinetobacter Baumannii Strain A92

Author name: سهاد سعد محمود العجيلي
Supervisor name: اليس كريكور ملكونيان | علي حسين ادحيه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

مقارنة الطرائق التشخيصية الوسط اللوني Chromogenic mediumوالانتشار بالاقراص DisK Diffusion المتسلسل PCR للكشف عن المكورات العنقودية الذهبية المقاومة للمثيسيلين في الردهات الجراحية في مدينة الناصرية == Comparison Of Chromogenic Medium, Disk Diffusion Test And Pcr Methods Forthe Detection Of Methicillin Resistant Staphylococcus Aureus (MRSA) In The Surgical Wards Of Al - Nasiriyah City

Author name: جواد رشید هویدي الزیدي
Supervisor name: امین عبد الجبار السلمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: English
University location: Basrah
First pages:

دراسة فسلجية لبعض انواع من جنس Bacillus المقاومة للمعادن الثقيلة وتحسين قابليتها الاصحاحية == Physiological Study Of Some Bacillus Spp. Resistant To Heavy Metals And Improving Their Ability Of Bioremediation

Author name: ایمان عبعوب مخیفي
Supervisor name: امین عبد الجبار السلمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: English
University location: Basrah
First pages:

عزل وتشخيص جرثومة Helicobacter pylori من مياه الصرف الصحي في محافظة البصرة باستعمال الطريقة الزرعية وتقنية ال CR ( 16S rRNA ) == Isolation And Identification Of Helicobacter Pylori From Sewage In Basrah Province By Culture Method And PCR (16S Rrna)

Author name: الاء يعقوب الربيعي
Supervisor name: امين عبد الجبار السلمي | اسعد محمد رضا الطائي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: English
University location: Basrah
First pages:

القرابة الوراثية لجراثيم Acinetobacter baumannii المعزولة من مدينة الطب في بغداد == Genotyping Relatedness Of Acinetobacter Baumannii Isolated From Medical City - Baghdad

Author name: نظيمة حمود حسين السليم
Supervisor name: حارث جبار فهد المذخوري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

دراسة تشخيصية لبكتريا Helicobacter pylori المسببة لمرض التقرحات المعدية في محافظة صلاح الدين == Diagnostic Study for Helicobacter pylori Bacteria That Cause Gastric Ulcers in Salah Aldeen Governorate

Author name: مصعب علي جواد المهداوي
Supervisor name: امين سليمان بدوي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

تاثير بعض مبيدات الادغال والحشرات في نمو ونشاط بكتريا العقد الجذرية في نبات الباقلاء Vicia faba == EFFECT OF SOME HERBICIDES AND INSECTICIDES ON GROWTH AND ACTIVITY OF ROOT NODULES BACTERIA ON Vicia faba PLANT

Author name: انمار سعدي عبود العكيدي
Supervisor name: نضال نعمة حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

عزل وتشخيص الجراثيم من عينات سريرية وبيئية والكشف عن بعض العناصر الوراثية المسؤولة عن المقاومة للمضادات الحيوية والعناصر الثقيلة في محافظة البصرة == Isolationand Identification of Bacteria from clinical and Environmental Samples and Detection of Some Genetic elements Responsible for Antibiotic and Heavy Metals Resistance in Basrah Government

Author name: اسماعيل جمعة عباس
Supervisor name: ميثم ايوب الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages:

التلوث بالبكتريا الهوائية المرضية المعزولة من الجروح ما بعد العمليات والحروق والتعرف على بعض مصادرها وخاصة الكلبسيلا الرئوية في محافظة ذي قار == The Contamination by Aerobic Pathogenic Bacteria Isolated from Postoperative Wounds and Burns and Study some of Their Sources Especially Klebsiella pneumoniae in Thi-Qar province

Author name: خوام ريسان حسين الحسيني
Supervisor name: Myad F. Nakkash | Ilham s. Abdul Karim
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Baghdad
First pages:

انتشار صفة المقاومة لمضادات البنسلينات والسيفالوسبورينات لبعض انواع البكتريا السالبة لصبغة غرام المنتجة لانزيمات البيتالاكتاميز عند النساء اللواتي يعانين من التهاب المهبل == Dissemination of Penicillins and Cephalosporins Resistance among Some Gram-Negative Bacteria, Producers of -Lactamases, in Women of vaginal Inflammation

Author name: وليد شمسي حسين المشهداني
Supervisor name: حسين حسن عمر خانقاه | فاروق خالد حسن العكيدي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

دراسة بعض الجوانب البكتيرية والمناعية لخمج المجري البولي المزمن == Study of Some Bacteriological and Immunological Parameters in Chronic Urinary Tract Infection

Author name: محمود ابراهيم اسماعيل
Supervisor name: خليل مصطفى خماس | علي حسين ادحيه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

التاثيــر البايولوجي لســموم بكتريا ذات الرئــة على الفئران البيضاء (داخل الجســم وخارجـــه ) == Biological Effects Of Klebsiella Pneumoniae Toxin In Albino Mice (In Vivo & In Vitro)

Author name: محمد سلمان محمد صريح
Supervisor name: عصام فاضـل الجميلــي | ناهــي يوســف ياســين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

دراسة بكتيريولوجية وكيموحيوية ونسيجية لتاثير حامض اللاجيك المستخلص من قشور الرمان على بعض عزلات بكتريا الكوليرا Vibrio cholerae وانزيم البروتييز == Bacteriological, Biochemical, and Histological study for the effect of Ellagic acid extracted from Punica granatum peals on some Vibrio cholerae isolates and protease

Author name: لقاء حميد مهدي الموسوي
Supervisor name: مهدي ضمد محيسن | سوسن حسن عثمان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

دراسة بكتريولوجية وكيموحيوية لبعض عوامل الضراوة المنتجة من بكتريا Vibrio cholerae المعزولة محليا من المرضى المصابين بالهيضة

Author name: كفاح احمد جاسم الكرخي
Supervisor name: غازي منعم عزيز | حسين حسن خانقاه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

دراسة بكتريولوجية، مرضية ونسيجية لحالات التهابات الزائدة الدودية == Bacteriological and Pathohistological Study on Appendicitis

Author name: غادة حسن برهان
Supervisor name: رعد خليل الحسيني | علاء غني حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Language: Arabic
University location: Baghdad
First pages:

دراسة جزيئية ووراثية لعوامل الضراوة لبكتريا Acinetobacter المعزولة من خمجات مرضية مختلفة

Author name: عباس شاكر جواد المحنة
Supervisor name: سوسن ساجد الجبوري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:

التحري عن وجود جرثومة Staphylococcus Saprophyticus في حالات اصابات الجهاز البولي في الانسان وبعض الحيوانات == Investigation about STAPHYLOCOCCUS SAPROPHYTICCUS in urinary tract infection in human and some animals

Author name: عبد الكريم سلمان صكبان اليساري
Supervisor name: حسن علي عبد الرضا | محمد صبري عبد الرزاق
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

عزل وتشخيص جرثومة Salmonella Typhimurium والكشف عن الجين المشفرلتخليق النوع الهدبي الاول باستعمال تقنية تفاعل سلسلة البوليمريز == Isolation and Identification of Salmonella typhimurium and detection of gene encoded Type - 1 - fimbriae by using Polymerase Chain Reaction

Author name: حيدر علي محمد الكرعاوي
Supervisor name: عدنان موسى الروضان | عدنان حمد الحمداني
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages:

عزل وتشخيص جرثومة Burkholderia pseudomallei من حليب الابقار والمعز والبيئة المحيطة بها في محافظة البصرة == Isolation and Identification of Burkholderia pseudomallei from Cow and Goat’s Milk and Their Surrounding Environment in Basrah Province

Author name: هناء خليل ابراهيم
Supervisor name: عدنان موسى الروضان
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة جرثومية مصلية عن اهم مسببات الاجهاض في النساء مع التركيز على داء البروسيللوسيز == Bacteriological and Serological Study of Important Causes of Abortion in Women with Concentrated on Brucellosis

Author name: لمى حكمت كريم البياتي
Supervisor name: غازي موسى الخطيب
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

Extraction And Purification Of Salmonella Spp. Enterotoxin Isolated From Bovine In Basrah Province

Author name: مؤيد حنون صيهود
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Basrah
First pages:
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