University location: Qadisiyah

First pages:

Abstract: The present study had been carried out to elucidate the toxic effects of sodium fluoride on local rabbit males, this study involved determination of the histological Changes of some organs include (Testes, Liver and Kidney) , moreover study the changes in body weight of the animals and the percentage of organs weight and some blood parameters and biochemistry which include packed cell blood volume (PCV), Hemoglobin (Hb), Total white blood cells, Testosterone hormone, T3, T4 hormones, Glucose concentration, Cholesterol, Urea, Transaminase enzymes and Bilirubin - level in blood. Twenty local adult male rabbits were included this experiment their ages ranged between 10 - 14 months.The animals were treated with oral swallow for 12 weeks. The animals were divided into four groups : control group is treated orally with (0.9%) normal saline; First group was treated orally with 10 mg/kg of body weight sodium fluoride, second group was treated orally with 20 mg/kg of body weight sodium fluoride and third group was treated orally with 30 mg/kg of body weight sodium fluoride. The results of present study showed a significant decreased (P<0.05) in body weight of treated animals with dose of sodium fluoride 20 and 30 mg/kg of body weight, while there is no significant differences in the body weights of treated animals with sodium fluoride dosage of 10 mg/kg of body weight when to compared with control group. The percentage of organ weight to body weight showed a significant decrease in testis weights and liver weight, while there was no significant differences in kidney weight in treated group with sodium fluoride when to compared with control group. Concerning histological changes showed a reduction or freezed stages in spermatogenesis processes which timed with decrease in number of primary spermatocytes and secondary spermatocytes in addition to infiltration of lymphocytes, also found that NaF cause disorganization, denudation, and reduction in germinal epithelial cells of the seminiferous tubules and associated with absence of sperm in the lumina, also showed a decrease in radius of epididymus ducts and decrease in number of sterocilia and decrease number of mature sperms. Histological sections of kidney showed that there was a clear shrinkage of glumeruli.Histological section of kidney of animals that treated with sodium fluoride showed a mild blood vessels congestion, shrinkage in glumeruli, distributed in renal tubules and erode its epithelium with some apoptotic areas. The liver sections appeared appreciable effect with fluoride include dilation in central vein and erode to its epithelium, congestion in hepatic sinusoids with apoptosis and erode to general structure of liver with some of inflammatory cells and death in some cells which lead to erode of typical ray arrangement. Regarding blood parameters the present study showed a significant decrease (P<0.01) in percentage of (PCV) and (Hb) of the animals treated with sodium fluoride, while showed (WBCs) count significant increased compared with control group. Hormonal analysis (Testosterone, T3 and T4) showed a significant decrease, while significant increase of glucose, cholesterol, urea, transaminases enzymes for (AST, ALT) and bilirubin levels. Results obtained from this study and mentioned above may be attributed essentially to that of Naf administration results in sever lesion of male reproductive system wich may be aggravated with increase administration concentration of Naf.

تقييم استخدام الانترليوكينات في تشخيص تسمم الدم الجرثومي لدى الاطفال حديثي الولادة في تكريت == Evaluation Uses The Interleukines For Diagnosis of Neonatal Sepsis In Tikrit

Author name: هبة حازم صالح

Supervisor name: موسى جاسم محمد الحميش | عاشور رفعت سرحت

General topic: Biology

Specific topic: Zoology

Degree: Master

University: Tikrit University - College Of Science - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: ان الهدف الرئيس لهذه الدراسة هواستخدام الانترلوكيناتInterleukin - 8, Interleukin - 10 (IL - 8, IL - 10) كعلامة تشخيصية للتسمم الدموي الجرثومي لدى حديثي الولادة. تضمنت الدراسة 45 عينة دم من الاطفال حديثي الولادة الراقدين في ردهة الاطفال في مستشفى تكريت الت | The main aim of this study was uses the Interleukines IL - 8 and IL - 10 as diagnosis marker for neonatal sepsis. This study involved 45 blood samples, were taken from neonates admitted to the pediatrics - neonatal wards at Tikrit Teaching Hospital who were clinically diagnosed as sepsis for the period from September 2011 until March 2012. The ages of neonates were 1 - 30day, and this blood samples were taken for the purpose of determining the levels Interleukines IL - 8 and IL - 10 and identify (WBC and C - reactive protein). Seventeen samples were taken from the blood of neonates without of septicemia as a control group samples. The results of the current study showed non significant differences in total white blood cells count for patients with sepsis when compared with a control group. and showed non significant differences in total white blood cells count for patients with sepsis as gestation birth, sex and birth weight compared to the control group. Regarding level of C - reactive protein there was no significant differences between the control group and the patients with sepsis as gestation birth and sex. and showed a significant differences in C - reactive protein level for patients compared to the control group as birth weight. The Interleukine levels showed that a significant increase in the level of interleukin - 8 (IL - 8) for all patients mals and femals, preterm or full - term and who there weight < 2.5 or >2.5kg compared with a control group. this results showed that the relationship between IL - 8 and WBCs was not significant differences at (p<0.05). and for the level of interleukin - 10 the present study showed a significant increase for patients with sepsis as gestation birth, sex and birth weight compared to the control group, the relationship between IL - 10 and WBCs was not significant differences at(p<0.05). The relationship between IL - 1o, IL - 8 and WBCs was not significant differences. when comparison The results as end result for sepsis show is the absence of significant differences (p<0.05) for patients who became good and who had complications compared with the control group. At last, the study concluded that the Interleukines IL - 8 and IL - 10 levels increase in early time for Inflammation ;So its agood diagnosis marker for neonatal sepsis.

تاثير اضافة عناصر الكالسيوم والمغنيسيوم والحديد الى الوسط الغذائي في حياتية خنفساء الطحين الصدئية Tribolium castaneum (Herbst) Tenebrionidaci) Coleoptera : ) == Effect of Calcium, Magnisium And Iron Elements With Various Nutreint Sources On Biology of Tribolium Castaneum (Herbst) Tenebrionidaci) Coleoptera : )

Author name: حوراء ياسين احمد عبد

Supervisor name: برهان مصطفى محمد الدليمي | عواد شعبان داود الناصري

General topic: Biology

Specific topic: Zoology

Degree: Master

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: تناولت الدراسة اختبار تاثير اضافة ثلاثة عناصرمعدنية الكالسيوم (Ca) المغنيسيوم (Mg) الحديد (Fe) في سطين غذائيين (الطحين والجريش) لصنفين من الحنطة (ابو غريب والعز) في حياتية خنفساء الطحين الصدئية الحمراء Tribolium castaneum (Herbst). اذ استخدمت العناصر بترا | This study includes testing the effect of addition of three elements (Ca, Mg, Fe) in flour and crushed partially debranned wheat for two kinds of wheat : Abu - ghareeb and Al - is in the life of Tribolium castaneum (Herbst).using the elements under different concentrations (0.01 - 0.03 - 0.05) g.to know their effects in Tribolium inculuding : (the number of eggs, period of incubation eggs, number of Larvers, number of pupal, number of external insect, new generation) after adding to nutreint base for the two kinds of wheat.This experiement must be in dark incubation at 35±2°C & relative humidity 75±5% during one generation the result show most important role of nutreint element in life of insect (the number eggs, period incubation eggs, number of Larvers, number of pupal, number of external insect, new generation). on side of period incubation eggs period generation there is not any mentioned effect and the most meaningful one is in kind of Al - Is comperad with Abu - ghareeb. It is noticed the maxi effect for Calisum element.It achieved the highest level in : (the number eggs, period incubation eggs, number of Larvae, number of pupal, number of external insect, new generation) to ratio of flour kind of Al - Is and achieved maxi number : (the number eggs, period incubation eggs, number of Larvers, number of pupal, number of external insect, new generation)in concentration (0.05)g.while the highest in ratio of generation agents in (Fluorine)element ratio(76.2) under concentration (0.03)g, it achieved the less ratio in the dead insect kind of Abu - ghareeb7s the highest in average number eggs, number of larvers for calisum element.while the highest value in average number of pupal, number of external insect, new generation to (Magnasium) element it is achieved the highest in number of eggs, number of Larvers, number of pupal, number of external insect, in concentration (0.05)g and the higher of average ratio new generation is (66) and the higher ratio new generation (79.3) is concentarte (0.03)g.It is noticed the relationship between nutreint element and life of insect is parallel, that is, concentrates increased, number of growing also increased except (Fluorine) element the relation is inversion. on side of crushed partially debranned wheat the effect is more meaningful in kind of Abu - ghareeb than in Al - Iz.Calicum element shows acceptable results, it appeared the higher in : (the number eggs, , number of Larvers, number of pupal, number of external insect) in Abu - ghareeb and new generation in Al - Iz. In the test of the effect of nutreint element agents in life of this insect according to the nutril base for both kinds.the result show that nutrient base of flour is more effective than the nutrient base crushed bartially debranned whaet in all steps of insect life for both kindc in flour maxi In flour maxi number in new generation about (62.5)g and the main ratio of new generation in crushed partially debranned wheat (36.3) for Abu - ghareeb kind (6.7)in flour. (61.1) crushed partially debranned wheat For Al - Is which shown the same features of the results of the effect in nutrient base without adding any elements to its life of insect

العلاقة المحتملة بين مرض الاكياس المائية وسرطان الكبد والرئتين == The Probable Relationship Between The Hydatidosis And Liver And Lungs Cancer

Author name: ايمان سلمان خميس محمود

Supervisor name: ندى محمد طه البشير | نغم ياسين البياتي

General topic: Biology

Specific topic: Zoology

Degree: Master

University: University of Diyala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Diyala

First pages:

Abstract: تعد الاصابة ببعض العوامل المرضية مسؤولة عن حوالي خمس حالات السرطان في العالم. وهنالك العديد من الاصابات الطفيلية التي ثبت دورها كمسبب او محفز لسرطانات معينة، الا ان العلاقة بين الاصابة بالاكياس المائية والسرطان مازالت موضع جدال ولذلك فقد هدفت الدراسة الح | Infectious agents are responsible for about one fifth of all cancer cases worldwide. Some parasitic infections are well documented to cause certain cancers. However, the association of hydatid cyst (HC) with cancer is a controversial issue. The current study aimed to investigate the possible association between chronic hydatid cyst infection with liver and lung cancers. The study involved three groups : the first group included 42 patients with HC, the second group included 35 patients with liver (12) and lung (23) cancers, while 25 apparently healthy individuals were recruited to represent the third group (control). Number, diameter, location and the status (calcified or non - calcified) of the HC were recorded from HC group. Blood samples were collected from the studied groups from which serum were separated and kept until being used. Enzyme linked immune - sorbent assay (ELISA) was used to estimate serum levels of anti - HC IgG antibodies, carbohydrate antigen (CA19 - 9), alkaline phosphatase (ALP) and ? - glutaml transferase (GGT), while Absorbance measured by spectrophotometric method was used to determine levels of lactate dehydrogenase (LDH) and adenosine deamimase (ADA).All members of HC group gave positive result for anti - HC IgG antibodies compared to 11 patients from cancer group and oneindividual from control group (Odds ratio =8.3). Both HC and cancer groups showed significantly higher p<0.05 levels of CA19 - 9, ALP, LDH and GGT ((76.16±58.26 U/ml and 110.77±96.47 U/ml), (131.81±76.65 U/L and 141.72±97.23 U/L), (244.24±117.94 U/L and 254.68± 115.56 U/L) and (95.06±66.73 U/L and 105.12± 41.42 U/L) respectively) than control group (10.81± 9.12 U/ml, 65.14± 25.21 U/L, 146.15± 37.59 U/L and 17.21± 6.87 U/L respectively). In cancer group, casp - 8 level (72.20±54.72 picomol/ L) was significantly lower from than that of control group (92.30± 41.20 picomol/L) and insignificantly from that of HC group (60.86±40.84 picomol/L). In HC group, ADA level was 47.84±10.65 U/L and was significantly higher p<0.05 than that of cancer group (10.14± 1.99 U/L) and control (16.09±2.70 U/L).Investigation for the risk factors which may increase the risk of developing liver or lung cancers in HC patients revealed that male, smokers and rural residents were more susceptible for such development (Odds ratios : 3.45, 6.982 and 5.48, respectively). Number and cyst size were significantly correlated with the level of anti - HC IgG antibodies (r= 0.539, P<0.001 and r= 0.618, P< 0.001 respectively), while calcified cysts associated with significantly higher levels p<0.05 of CA19 - 9 and GGT (105.03±88.9 U/ml and 145.46±101.57 U/L, respectively) than that of non - calcified cysts(65.91±39.87 U/ml and 77.17±7.23 U/L, respectively). Average level of anti - HC IgG antibobodies in calcified cysts was 19.75±13.11U/ml and was significantly P<0.05 lower than its counterpart of non - calcified cysts (85.017±76.84 U/ml). All studied parameters showed significantly higher levels in livers cysts than their counterparts in lung cysts. Results of the current study indicate that chronic infection with HC can increase the host susceptibility to develop liver or lung cancer.

دراسة فعالية المشتت الحيوي السطحي المستخلص من بكتريا Bifidobacterium spp في تثبيط الغشاء الحيوي للمسببات المرضية المعزولة من مرضى القسطرة القلبية وتاثيره في عملية البلعمة == Study of The Effectiveness of Biosurfactant Extracted From Bacteria Bifidobacterium Spp. In The Inhibition of Biofilm of Pathogens Isolated From Cardiac Catheterization Patients And Its Effect In Phagocytosis

Author name: بتول شاكر عبد المجلاوي

Supervisor name: هيام عبد الرضا كريم العواد | علي رحيم حنظل الهامل

General topic: Biology

Specific topic: Zoology

Degree: Master

University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

Abstract: This study was conducted in order to assess the effect of Biosurfactant extracted from bacteria Bifidobacterium spp in the inhibition of Biofilm produced by pathogenesis bacterial isolated from patients with during cardiac catheterization, This study getting 89 patients with cardiac catheterization unit in AL - Imam AL - Hussain Teaching Hospital from both genders of different ages ranged between (29 - 75) years, starting from (January 2014 and to the end of July 2014). The samples taken from atherosclerosis patients were cultured for all types of cardiac catheterization by three tests Swabs, catheter tip culture and blood culture sample before and after a catheter for the purpose of isolating pathogens that component of the biofilm of patients with atherosclerosis. The results of the current study showed that patients with diagnostic cardiac catheterization rate of 66 (74.16%) patients were given 15 (60%), 15 (60%), 14 (58.33%) and 15 (62.5%) bacterial growth, respectively. But patients with therapeutic cardiac catheterization of 15 (16.85%) patients were given 6 (24%), 6 (24%), 6 (25%) and 6 (25%) bacterial growth respectively, and diagnostic and therapeutic cardiac catheterization for together 8 (8.99 %) patients were given 4 (16%) 0.4 (16%), 4 (14.67%) and 3 (12.5%), bacterial growth, respectively. The results showed sensitivity and specificity values for swabs test 96%, 100%. while the catheter tip culture was 96% and 100% respectively, and blood culture was 100 and 98%, respectively, it became clear from the results after the sample cultivations on the enrichment and differential media that 89 patients (sample joint) gave 25 (28.08%) positive sample for bacterial cultivation, and two of them showed two types of bacteria to become 27 isolated bacterial, isolates were diagnosed 11 (70.74%) gram positive, 15 (55.56%) gram negative , one isolate only of the yeasts 1 (3.70%), and 64 (71.91%) did not give any growth. Ability of pathological isolates were tested for the production of Biofilm by using three different methods, tube method TM, Congo Red Agar CRA, microtiter plate M TP were given 25 (92.6%), 16 (58.26%) and 26 (96.29%) the isolate of producing biofilm by different quantities respectively. The results of the investigation of formation biofilm test showed that MTP has 100% sensitivity and 96% specificity, the TM sensitivity was 96% and 100% specificity and CRA was sensitivity of 61.5% and 100% specificity. One hundred and fifteen samples were collected from of local and imported dairy products 45 (39.13%), breast milk 32 (27.83%) and cows milk, 38 (33.04%) were obtained at 22 (19.13%) positive sample of Bifidobacterium bacteria after morphological, microscopic and biochemical diagnosis tests and comparing these isolates with standard isolates. Oil Spreding Techingue and blood Hemolysis was performed to investigate the capability Bifidobacterium on the production biosurfactant, the efficiency antithesis test was studied of liquid bacterial farm for Bifidobacterium bacteria against bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, E coli, Klebsiella pneumoniae , and Serratia marcescens was diameters of inhibition zones (24.25, 21, 24, 22, 23) mm respectively. The inhibitory effectiveness was Studied for filtrate against bacteria Staphylococcus epidermidis, S.aureus, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, P.aeruginosa, Enterobacter Cloacae and Serratia mercescens, where the E.coli bacteria was more effective and P.aeruginosa less effective by the bacterial filtrate. The study included the effect of three concentrations of biosurfactant against bacteria test group, and the diameters of inhibition rates of inhibition areas to concentrations 50% as follows (33.34, 30.34, 32.30, 33.32) mm respectively. The diameters rates of zones of inhibition for the concentration of 25% was as follows (30.32, 27.32, 31.28, 31.5, 29) mm respectively, and zones inhibition rates of concentration of 12.5% as follows (28.29, 24.30, 26.26, 28.26) mm respectively, statistically significant differences at the significance level of P <0.01inhibition in rates depending on the type of user concentration and statistically significant differences between the types of bacteria in their affected by biosurfactant. also determine the values of (MIC) Minimum Inhibitory Concentraction, (SubMIC) Sub Minimum Inhibitory Concentraction and (MBC) Minimum Bactericidal Concentraction, the value of MIC 6.25 mg / ml, Sub MIC 3.12 mg / ml and MBC 12.5 mg / ml of the bacteria E.coli, K. pneumoniae, Enterobacter cloacae, and the value of MIC amounted to 12.5 mg / ml, Sub MIC 6.25 mg / ml and MBC 25 mg / ml of bacteria S.aureus, S.epidermidis, P. mirabilis, P. aeruginosa and Serratia mercescens. The susceptibility of biosurfactant was studied to inhibit biofilm bacteria by MTP method The results showed a significant decrease in the formation of biofilm after the addition of biosurfactant compared with the biofilm before adding biosurfactant where the decreased rate reached 96%. Effect of biosurfactant test was performed on the efficiency of phagocytosis in vitro the percentage of phagocytosis reached 40.9% of the control group 64.8% of the experimental group. Concluded that the biosurfactant has a clear inhibitory efficacy against pathogens isolated from patients with cardiac catheterization and inhibition of biofilm for pathogens as well as raising the efficiency of the process of phagocytosis in vitro

Author name: سارة حاتم عجيل خضير

Supervisor name: داخل غاني عمران الوطيفي

General topic: Biology

Specific topic: Zoology

Degree: Master

University: University of Babylon - College Of Science For Girls - Department Of Biology

Language: Arabic

University location: Babylon

First pages:

Abstract: يتميز سن الياس (Menopause) لدى الاناث بتوقف الحيض وتصبح الانثى غير خصبة بسبب فقدان جميع الحويصلات المبيضية (Ovarian follicles) والانخفاض الشديد لهرموني الاستروجين والبروجسترون صممت الدراسة الحالية لفحص الاضطرابات الفسلجية المختلفة والتي تشمل بعض المعايير | Menopause marks the time in women's life when her menstruation stop and she is no longer fertile because of exhaustion of ovarian follicles and sharply decrease in ovarian production of estrogen and progesterone.The present study was designated to determinate different physiological disturbances which occurring in some hematological and biochemical parameters of postmenopausal women. A total number used was 130 women, 100 women were postmenopause and subdivided into four age groups (46 - 50 year, 51 - 55 year, 56 - 60 year, and 61 - 65 year). Thirteen women were premenopause , used as a control group, their ages ranged between 20 - 30 years. In all age groups of postmenopausal women, it have been found that the levels of estradiol hormone were progressioly decreased (p<0.05) when compared with control group. Regarding hematological parameters, RBCs count was insignificantly increase (p>0.05) in all age groups of postmenopausal women. Levels of hemoglobin concentrations and packed cell volume were significantly increased (p<0.05) in all age groups of postmenopausal women compare to control groups. Blood platelets count recorded insignificant fall (p>0.05) in most age groups of postmenopausal women.As for, red blood indices (MCV, MCH, and MCHC) explained insignificant differences (p>0.05) of most menopausal women in a comparison with those control group. Total white blood cells have insignificant elevation (p>0.05) of most groups except 56 - 60 years groups. Regard to differential white blood cells, Levels of lymphocytes and monocyte have been showed insignificant drop (p>0.05) of the level of lymphocytes and significant increase (p<0.05) of first and second age groups in the levels of monocytes of postmenopausal women. Granulocytes white blood cells have been indicated different changes : Nentrophils were significantly higher (p<0.05)in all age groups of postmenopausal women, where as, eosinophils and basophils were insignificantly different (p>0.05) in most age groups. Concerning biochemical changes, Total serum proteins, Albumin, and globulin have been showed significant lowering (p<0.05) in all age groups of postmenopausal women. Statistical analyses showed a significant rise (p<0.05) of serum uric acid and urea levels in most group of postmenopausal women. Measurments of enzyme activities involved in liver (ALT and AST) were significantly hightened (p<0.05) in most age groups of postmenopausal women, Whil, ALP activities recorded insignificant increase (p>0.05) in all age group of postmenopausal women. Determinations of minerals showing a significant increase (p<0.05) in the levels of calcium and insignificant increase (p>0.05) in the levels of phosphorus in all groups of post menopausal women. Analyses of electrolytes have been explained a significant elevation (p<0.05) of the sodium and non significant difference (p>0.05) of the potassium in all groups of postmenopausal women.The present study involved determination specific and essential trace elements, Levels of copper explained a significant decrease (p<0.05) in all age groups associated with a significant increase (p<0.05) of zinc levels in postmenopausal women. Inversly, Values of magnesium were insignificantly different (p>0.05) in all age groups of postmenopausal women. The present study selects specific parameters to explaine oxidative stress in postmenopausal women. Levels of malondeheyds were significantly elevated (p<0.05) in all groups of postmenopausal women, and correlated negatively (r= - 0.102) with level of estrogen. Values of reduced glutathione showed a significant decrease (0.05) in postmenopausal women and correlated positively (r=0.64) with the estrogen hormone. Analyses of antioxidants enzymes have been showed a significant decrease (p<0.05) in the activity of catalase enzymes of all groups of postmenopause. Also, the activity of glutathione peroxidase was significantly decrease (p<0.05) in postmenopausal women and its correlation coefficient was r=0.263. In conclusion, changes summarized above may be attributed to deficiency of estradiol hormone in postmenopausal women which resulting in different physiological disturbances and these changes become aggravated and exacerbated because of oxidative stress occurring in decrease of estradiol hormones.