اسم المؤلف: سعاد تريجي زامل العكيلي

اسم المشرف: نجاح رايش هادي الموسوي فاضل غالي يوسف العمران

الموضوع العام: الطب

السنة: 2014

الموضوع الدقيق: الادوية والسموم

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية الطب - قسم الادوية والعلاجيات

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: يمثل نقص ارواء عضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. الانجيوتنسين الثاني قد يساهم في الاصابة بسبب اعادة الارواء عن طريق زيادة الاكسدة والعوامل الالتهابية. الانجيوتنسين الثاني يمارس معظم ا | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem following thrombolysis, angioplasty and coronary bypass surgery. Angiotensin II may contribute to reperfusion injury by increasing oxidative stress and inflammatory factors. Ang II exerts most of its effects via AT1Rs. Objective : This study was undertaken to investigate the potential role of Telmisartan and AT1 - AB in amelioration of myocardial I/R injury induced by ligation of coronary artery in mouse model. Materials & method : Adult male Swiss - albino mice were randomized into 6 equal groups. Group (1) sham group : Mice underwent the same anesthetic and surgical procedure as the active control group except ligation of LAD coronary artery.Group( 2) active control group : Mice were subjected to regional ischemia for 30 min by ligation of LAD coronary artery and reperfusion for 2 hours.Group( 3) control vehicle group (1) : Mice in this group injected with DMSO (vehicle for Telmisartan ) via IP route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion for 2 hr. Group( 4) control vehicle group (2) : Mice injected with D.W ( vehicle for AT1 - AB) via IV route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion fore 2 hr. Group (5)Telmisartan treated group : Mice pretreated with Telmisartan 0.5mg/kg i.p 30 minutes before ligation of LAD coronary artery. Group(6) AT 1 - AB treated group : Mice pretreated with AT 1 - AB (1Mcg/gm.) of body weight via IV route 30 minutes before ligation of LAD coronary artery. Results : Compared with the sham group, Levels of TNF - ? & IL - 1?, IL - 6,caspase 3 and plasma level of cardiac troponin I increased in control group (p<0.001).Levels of Bcl2 decreased in control group(p<0.001). Histologically ,All mice in control group showed a significant (p<0.001) cardiac injury. Both Telmisartan and AT1 receptor antibody significantly counteract the increase in myocardium level of TNF - ?, IL - 1B,IL - 6,caspase 3 ,plasma cTnI (P < 0.001). Furthermore, the Telmisartan and AT1 receptor antibody significantly increased in myocardium level of Bcl2. Histological analysis revealed that both Telmisartan and AT1 receptor antibody markedly reduced (P < 0.001) the severity of cardiac injury in the mice underwent LAD ligation procedure. Conclusion : The results of the present study reveal that Telmisartan and AT1 receptor antibody ameliorate myocardial I/R injury in Mice via interfering with inflammatory reactions & apoptosis which induced by I/R injury.

تاثير الايتانرسبت على السايتوكينات وموت الخلايا المبرمج جراء انقاص الارواء الدموي واعادته لعضلة القلب في ذكور الفاران Effect of Etanercept Against Myocardial Ischemia/Reperfusion Injury In Male Mice

اسم المؤلف: سيف محمد حسن

اسم المشرف: نجاح رايش هادي الموسوي فاضل غالي يوسف العمران

الموضوع العام: الطب

السنة: 2014

الموضوع الدقيق: الادوية والسموم

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية الطب - قسم الادوية والعلاجيات

اللغة: الانكليزية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: يمثل نقص ارواءعضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. تشمل اصابة عضلة القلب بسبب نقص التروية الدمويه واعادتها ضعف مقلص القلب، عدم انتظام ضربات القلب وكذلك تلف الخلايا العضلية التي لا رجعة في | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem associated with thrombolysis, angioplasty and coronary bypass surgery. Injury of myocardium due to ischemia - reperfusion includes cardiac contractile dysfunction, arrhythmias as well as irreversible myocytes damage. These changes are considered to be the consequence of imbalance between the formation of oxidants and the availability of endogenous antioxidants in the heart. Objective : This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model.Material & method : adult male Albino mice were randomized into four equal groups.1. Group (1) : Sham group : mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery.2. Group (2) : Control group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours.3. Group (3) : Control vehicle group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice received vehicle of etanercept (normal saline) 5 minutes before reperfusion via I.P injection and.4. Group( 4) : Etanercept treated group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice treated with etanercept 5mg/kg i.p 5 minutes before reperfusion XVIIIResults : Compared with the sham group, the levels of TNF - ? & IL - 1?, IL - 6, Caspase 3 and plasma level of cardiac troponin I increased in the control group but decreased level of Bcl - 2 (p<0.01).Histologically, all mice in the control group showed significant (p<0.01) cardiac injury and apoptosis.Etanercept significantly decreased in myocardium level of TNF - ?, IL - 1B, IL - 6,Caspase 3 , and plasma cTnI (P < 0.01), while significantly increased level of Bcl - 2 (P < 0.01). Histological analysis revealed that etanercept markedly reduced (P < 0.01) the severity of cardiac injury in the mice underwent LAD ligation procedure. : ionsConcluThe results of the present study reveal that etanercept may ameliorate myocardial I/R injury in mice via interfering with inflammatory reactions and apoptosis. : RecommendationAfter studying the results of the present study, the following recommendation to further1. Further measuring the P - selctine and E - selectine to show the effect of etanercept on rolling of eutrophils and platelets that cause further occlusion of blood vessels.2. Further measure the adiponectine (that have a cardioprotective effect ).