تاثير التلميزارتان والاجسام المضادة لمستقبلات الانجيوتنسين نوع 1 في انقاص الارواء الدموي واعادته لعضلة القلب وموت الخلايا المبرمج في ذكور الفئران == Effects of Telmisartan And Angiotensin II Type1 Receptor Antibody In Myocardial Ischemia/Reperfusion Injury And Apoptosis In Male Mice
Author name:
سعاد تريجي زامل العكيلي
Supervisor name:
نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic:
Medicine
Specific topic:
Medicines and Toxins
Degree:
Master
University:
University of Kufa - Faculty Of Medicine - Department Of Medicine And Therapeutics
Language:
English
University location:
Najaf
First pages:
19T1110 - p.pdf
Abstract:
يمثل نقص ارواء عضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. الانجيوتنسين الثاني قد يساهم في الاصابة بسبب اعادة الارواء عن طريق زيادة الاكسدة والعوامل الالتهابية. الانجيوتنسين الثاني يمارس معظم ا | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem following thrombolysis, angioplasty and coronary bypass surgery. Angiotensin II may contribute to reperfusion injury by increasing oxidative stress and inflammatory factors. Ang II exerts most of its effects via AT1Rs. Objective : This study was undertaken to investigate the potential role of Telmisartan and AT1 - AB in amelioration of myocardial I/R injury induced by ligation of coronary artery in mouse model. Materials & method : Adult male Swiss - albino mice were randomized into 6 equal groups. Group (1) sham group : Mice underwent the same anesthetic and surgical procedure as the active control group except ligation of LAD coronary artery.Group( 2) active control group : Mice were subjected to regional ischemia for 30 min by ligation of LAD coronary artery and reperfusion for 2 hours.Group( 3) control vehicle group (1) : Mice in this group injected with DMSO (vehicle for Telmisartan ) via IP route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion for 2 hr. Group( 4) control vehicle group (2) : Mice injected with D.W ( vehicle for AT1 - AB) via IV route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion fore 2 hr. Group (5)Telmisartan treated group : Mice pretreated with Telmisartan 0.5mg/kg i.p 30 minutes before ligation of LAD coronary artery. Group(6) AT 1 - AB treated group : Mice pretreated with AT 1 - AB (1Mcg/gm.) of body weight via IV route 30 minutes before ligation of LAD coronary artery. Results : Compared with the sham group, Levels of TNF - ? & IL - 1?, IL - 6,caspase 3 and plasma level of cardiac troponin I increased in control group (p<0.001).Levels of Bcl2 decreased in control group(p<0.001). Histologically ,All mice in control group showed a significant (p<0.001) cardiac injury. Both Telmisartan and AT1 receptor antibody significantly counteract the increase in myocardium level of TNF - ?, IL - 1B,IL - 6,caspase 3 ,plasma cTnI (P < 0.001). Furthermore, the Telmisartan and AT1 receptor antibody significantly increased in myocardium level of Bcl2. Histological analysis revealed that both Telmisartan and AT1 receptor antibody markedly reduced (P < 0.001) the severity of cardiac injury in the mice underwent LAD ligation procedure. Conclusion : The results of the present study reveal that Telmisartan and AT1 receptor antibody ameliorate myocardial I/R injury in Mice via interfering with inflammatory reactions & apoptosis which induced by I/R injury.
