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تاثير بعض المضادات الفطرية والاعشاب الطبية على الفطريات الجلدية المعزولة من سعفة الراس في مدينة الديوانية == Effect of some antifungals and medicinal herbal against dermatophytes isolated from Tinea capitis in Al-Diwaniya governorate

Author name: وسام جاسم عبد علي
Supervisor name: منى حمودي خماس
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:

دراسة مظهرية وتشريحية مقارنة لانواع في اجناس معينة من العائلــــــــة المركبــــة Compositae (Asteraceae) == A Comparative morphological and anatomical study of selected species in certain genera from the family compositae(Asteraceae

Author name: رشا عودة جعفر الزبيدي
Supervisor name: خليل ابراهيم عباس الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:

دراسة تصنيفية مقارنة لمراتب الجنس Scutellaria L. (Labiatae) في العراق == A COMPARATIVE SYSTEMATIC STUDY TO THE TAXA OF THE GENUS Scutellaria L. (LABIATAE) IN IRAQ

Author name: علي حالوب كاظم معله
Supervisor name: ميسون خضر عباس البياتي
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:

استخلاص بعض المركبات الفعالة في نبات الرازقي Jasminum sambac L. المستزرع ودراسة فعاليتها في الخلايا السرطانية في الزجاج باستخدام الاجسام النانوية == Extraction of some active compounds in cultivated Jasminun sambac L. and study its activities against cancer cells in vitro by using nanoparticals

Author name: هديل مكي حبيب المؤمن
Supervisor name: بشرى محمد جابر علوش | محمد عبد الهادي غالي
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: هدفت الدراسة الى اختبار فاعلية المركبات الفعالة لنبات الرازقي Jasminum sambac ضد الخطوط السرطانية وزيادة كفاءة هذه المركبات باستعمال تقنيات النانوتكنولوجي. استخلصت المركبات الفعالة من جميع اجزاء النبات باستعمال اربعة مذيبات عضوية هي الكلوروفورم 99.4% والميثانول 99.8% و70% الميثانول والايثر البترولي, وقد كشف عن هذه المركبات نوعيا باستعمال الكشوفات الخاصة وتقنية TLC ، وكشف عنها كميا باستعمال تقنية HPLC. عزل مركب Jasminin iridiod glycoside ونقي وحسبت كميته في المستخلصات الاربعة باستعمال تقنية HPLC . اختبرت الفعالية السمية للمستخلص الكلوروفورمي وJasminin iridiod glycoside على الخطين الخلويين السرطانيين HeLa وRD والخط الخلوي الطبيعي REF باستعمال التراكيز 0.0 و7.81 و15.6 و20 و25 و31.25 و62.5 او 125 مايكروغرام/ مل ولمدة تعريض 24 ساعة، اذ حضرت جسيمات اللايبوسوم النانوية (liposome) بطريقة التجفيف - اعادة الترطيب (dehydration - rehydration) وباستعمال DMPC : Cholesterole بنسبة (50 : 45) ملغم/مل، وحملت بالمستخلص الكلوروفورمي او Jasminin بتركيز 50 مايكروغرام/ مل، وتم التحري عن كفاءة تحميل اللايبوسوم باستعمالHPLC وحدد حجم جسيمات اللايبوسوم باستعمال DSL ، وكشف عن وقيست شحنة اللايبوسوم باستعمال Zeta - Potential، وحدد شكل اللايبوسوم باستعمال المجهر اللالكتروني الماسح SEM، كما اختبرت سمية المستخلص الكلوروفورمي او الـ Jasminin المحملة على الاجسام الدهنية النانوية على الخطوط الخلوية السرطانية HeLa وRD والخط الخلوي الطبيعي REF مقارنة بالمستخلص الكلوروفورمي والـ Jasminin فقط ومجموعة السيطرة. اظهرت النتائج ان المستخلصات الاربعة (الكلوروفورمي والميثانولي99.8 و70% ميثانولي الايثر البترولي) تحتوي على القلويدات والفلافينويدات والتانينات والصابونيات والتربينات، وكانت كمية الـ Jasminin في المستخلص الكلوروفورمي والمستخلص الميثانولي وو المستخلص 70% ميثانول والمستخلص الايثر البترولي هي 111.38 ، 62.69 ، 61.50، 25.88 ومايكروغرام/ مل على التوالي من كل 100 ملغم/لتر. وكانت نتائج تحديد السمية ضد الخطوط الخلوية ان التركيز 125 مايكروغرام /مل للمستخلص الكلوروفورمي قد تسبب في اعلى نسبة تثبيط لخلايا الخطين الخلويين السرطانيين (HeLa وRD ) اذ بلغت في خلايا الخط الخلوي HeLa 72.51 وكانت في خلايا الخط الخلوي RD 64.28.في حين ادى استعمال الـ Jasminin الى نسبة تثبيط بلغت اقصاها باستعمال التركيز 125 مايكروغرام/ مل ضد خلايا الخط الخلوي HeLa 72.60 بينما كانت 67.27 ضد خلايا الخط الخلوي RD ، ولم يكن للمستخلص الكلوروفورمي والـ Jasminin تاثيرا تثبيطيا ضد خلايا REF. كانت شحنة اللايبوسوم المحمل بالمستخلص الكلوروفورمي - 44.84 وحجمها 84.7. اما باستعمال الـ Jasminin فقد وصلت الى - 57.00 وبلغ حجمها 70.3 نانوميتر ، وظهرت جسيمات اللايبوسوم عند فحصها بالمجهر الالكتروني الماسح SEM كروية الشكل، اما تركيز المستخلص الكلوروفورمي فقد كان 20 مايكروغرام/مل في اللايبوسوم، وبلغت كفاءة تحميل اللايبوسوم له 40%، اما تركيز الـ Jasminin فقد كان 25.42 مايكروغرام/ مل في اللايبوسوم ووصلت كفاءة تحميله الى 50.84%. اظهرت نتائج سمية اللايبوسوم المحمل بالمستخلص الكلوروفورمي او الـ Jasminin ولثلاث مدد تعريض (24 و48 او 72 ساعة)، ان اعلى نسبة تثبيط كانت لللايبوسوم المحمل بالمستخلص الكلوروفورمي لخلايا الخط الخلوي HeLa وبلغت 84.31 مقارنة بنسبة 80.57 لخلايا الخط الخلوي RD وذلك بعد مرور 48 ساعة من التعريض. اما اللايبوسوم المحمل بالـ Jasminin فسجل اعلى تثبيط في خلايا الخط الخلوي HeLa والذي بلغ 80.90 ثم خلايا الخط الخلوي RD 87.18 وذلك بعد مرور48 ساعة من التعريض. | This study investigated the activity of natural compounds of the Jasminum sambac L. against cancer cell lines, and increased the active compounds efficiency by using nanotechnology. The active compounds were extracted by using four organic solvents (chloroform 99.4, methanol 99.8, 70% methanol and petroleum ether), detected qualitively by using special reagent and TLC technique, and quantitively by using HPLC technique. The Jasminin iridoid glycoside was isolated, purified accounted in four by using the HPLC technique. The cytotoxic activity of chloroform extract and Jasminin was tested on two cancer cell lines HeLa and RD, and the normal cell line REF by using the concentrations 0.0, 7.81, 15.6, 20, 25, 31.25, 62.5, or 125 µg/ml, for each one for 24 hours exposure time. Liposome was prepared by the dehydration - rehydration method by using DMPC : Cholesterol as (50 : 45) mg/ml ratio and loaded with 50 µg/ ml of chloroform extract or Jasminin. The liposomic encapsulated efficiency was detacted by using HPLC technique, liposome size by DSL. Detected the charged type and measure the liposome charged by zeta potential, and liposome shape were measured by using scanning electron microscope SEM, and the cytotoxic activity chloroformic extract or Jasminin on which loaded on liposome nanoparticals was tested on the cancer cell lines (HeLa and RD) and the normal cell line (REF) compared with unloaded extract and control cells. The results showed that the four extracts chloroformic, methanolic, 70% methanol and petroleum ether containing alkaloids, flavonoids, trpinoid, taninins and saponnins. The account of jasminin in chloroformic extract, methanolic extract,70% methanolic extract and petroleum ether extract was 111.38, 62.69, 61.50 and 25.88 µg/ ml respectively from 100mg/l. The results of cytotxicity against cell lines showed that the concentration 125 µg/ ml of chloroformic extract caused the highest inhibition percentage for the two cancer cell lines which reaches in HeLa cells line 72.51 and RD cells line 64.28, whereas the use of jasminin caused the maximum inhibitory percentage at the concentration 125 µg/ ml against HeLa cells line 72.60 and 67.27 for RD cells line, and there was no high inhibitory effect on REF cells line. The zeta potential of liposome loaded with chloroformic extract recorded - 44.84 and its size is 84.7 nm, whereas by useing of Jasminin it reached - 57.00 and its size was 70.3 nm. The scanning electron microscope (SEM) shows the spherical shape of liposome, whereby the concentration of chlroformic extract was 20µg/ ml in liposome, and the liposome encapsulated efficiency was 40%. The concentration of jasminin, on the other hand, was 25.42µg/ml in liposome and the encapsulated efficiency reaches 50.84%. The results of cytotxicity of liposome loaded with chloroformic extract and jasminin for three exposure times (24, 48 and 72) hours showed that the highest inhibition percentage in the liposome loaded with chloroformic extract for HeLa cells line recorded 84.31 and RD cells line was 80.57 after 48 hours from exposure. The liposome loaded with jasminin, by contrast, recorded the highest inhibition percentage in HeLa cells line 80.90 and RD cells line 87.18 after 48 hours from exposure.

تاثير حامض الجبرلين والبراسينولايد في بعض صفات النمو والحاصل لصنفين من نبات الشبنت Anethum graveolens L. == Effect of GA3 and Brassinolide on Some growth and Yield Parameters for two Cultivars of Plant Anethum graveolens L

Author name: وائل شاكر حميد الجبوري
Supervisor name: ماهر زكي فيصل الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت تجربة حقلية في الحديقة النباتية العائدة لقسم علوم الحياة، كلية التربية للعلوم الصرفة - ابن الهيثم - جامعة بغداد، في الموسم النمو 2015 - 2016 لدراسة تاثير تركيز حامض الجبرلين والبراسينولايد وتداخلهما في بعض الصفات المظهرية والكيميائية والمركبات الفعالة طبيا والحاصل ومكوناته في صنفين نبات الشبنت هما الصنف المحلي والصنف الهولندي. نفذت التجربة باستخدام تصميم القطاعات العشوائية الكاملة Randomized Complete Block Design (RCBD) وبثلاثة مكررات اذ تضمنت الدراسة صنفين نبات الشبنت واضافة خمسة تراكيز من البراسينولايد هي (0 و0.5 و1 و2 و3) ملغم.لتر - 1 ورش حامض الجبرلين على المجموع الخضري بتركيز 50ملغم.لتر - 1 وبدون رشه. وبهذا تكون التجربة عاملية (2 × 2 × 5 ×3) اذ تضمنت 60 وحدة تجريبية، وسمدت الوحدات التجريبية وفق معاملاتها. ويمكن ايجاز النتائج التي تم الحصول عليها كما ياتي : 1. اختلاف صنفي نبات الشبنت (المحلي والهولندي) عن بعضهما في الصفات المدروسة، فقد تفوق الصنف المحلي على الصنف الهولندي في صفة ارتفاع النبات ووطول الحامل الزهري وتركيز الفسفور ونسبة الكاربوهيدرات في المجموع الخضري ونسبة الكاربوهيدرات في الثمار ووزن 1000ثمرة. فيما تفوق الصنف الهولندي على الصنف المحلي في صفة طول الجذر وحجم الجذر وقطر الساق والوزن الرطب والوزن الجاف ومعدل النمو المطلق واستدامة الكتلة الحيوية وعدد النورات الزهرية وحاصل الثمار في النبات الواحد وحاصل الثمار في الوحدة التجريبية الواحدة وحاصل الثمار الكلي وتركيز النتروجين في المجموع الخضري وتركيز الكالسيوم وتركيز البوتاسيوم وتركيز المغنيسيوم ونسبة البروتين في المجموع الخضري ومحتوى الكلوروفيل الكلي في الاوراق وتركيز النتروجين في الثمار، نسبة البروتين في الثمار ونسبة الزيت والوزن النوعي.2. اظهرت الدراسة ان المعاملة بحامض الجبرلين والبراسينولايد والتداخل بينهما ادى الى زيادة معنوية في الصفات المدروسة ، عند رش 50 ملغم.لتر - 1 من حامض الجبرلين وتركيز 2 ملغم.لتر - 1 في معظم الصفات والبعض منها عند التركيز 1 ملغم. لتر - 1 من البراسينولايد على المعاملات الاخرى. 3. اظهرت نتائج الفحص والتشخيص الكروماتوغرافي باستعمال تقنية HPLC تشخيص ستة مركبات فعالة في الزيت الطيار لبذور صنفي نبات الشبنت هي : (Anethol وLimonene وCarvone وDillapiole وP - anisaldehyde وLinalool). وقـد تـفوق الصنـف المحــلي على الصنـــف الهـولـندي باعطائــه اعــــلى تركـيــز لكل مــن المركبات الفــــعالـــة هـــما P - anisaldehyde) وLinalool)، عند رش حامض الجبرلين بتركيز 50 ملغم . لتر - 1 والبراسينولايد بتركيز1 ملغم. لتر - 1 ، بينما تفوق الصنف الهولندي على الصنف المحلي باعطائـه اعلى تركيز للمركبات الفعالة وهي : (Anethol وLimonene وCarvone وDillapiole) عند رش 50 ملغم. لتر - 1 الجبرلين و2 ملغم. لتر - 1 البراسينولايد. | A Field study was conducted at the farm of Biology Department, College of Education (Ibn AL - Haitham) for pure science, University of Baghdad during the growth season 2015 - 2016, on study the effect of Gibberellic acid concentration and brassinolide and their intraction on both morphological and chemical characteristics, the components of yield, determining the medically active compoands in two varieties of Dill plant, local and Holand varieties.The experiment was designed as Randomzied Complete Block Design (R C B D) with three replications. which two varieties of Dill plant and spray of Gibberellic acid (50) mg.liter - 1 and non spray and five concentration of brassinolide (0, 0.5 , 1,2 and 3) mg.liter - 1 The experiment was as designed factorial experiment (2x2x5x3) included sixty experimental units The plants were managed fertilized recommaded.Can be summarized the results that were obtained as follows : 1.different the variety of dill plant (local and holand) from each other in the traits, the local variety surpassed the holand variety in the plant height, the length of the pregnant syphilis, Concentration of phosphate, the percentage of carbohydrates in the shoot, the percentage of carbohydrates in the fruits, weight of a thousand fruits. As outweigh the holand variety at the local variety in the character of the root length, root size, stem diameter, wet weight, dry weight, the absolute growth rate, the sustainability of biomass, the number of inflorescences, holds fruits per plant, holds the fruits in a single experimental unit, holds overall fruits, Concentration nitrogen in the shoot, calcium Concentration, potassium Concentration, the percentage of protein in the shoot, the total chlorophyll content in the leaves, the Concentration of nitrogen in the fruits, the percentage Albroonin in fruits, oil content, specific weight.2. The study showed that treatment with acid and Gibberellin and brassinolide and overlap between them led to a significant increase in the traits, at a spray 50 mg.L - 1 Gibberellin acid and concentration of 2 mg.L - 1 in most qualities and some of them at a concentration of 1 mg.L - 1 brassinolide the rest of the transactions.3. Screening and diagnosis chromatographic results showed the use of HPLC technique diagnosed six compounds in the volatile oil fruit plant varieties Dill are : (Anethol , Limonene , Carvone , Dillapiole , P - anisaldehyde and Linalool). Local variety was higher than the holand variety by giving it the highest concentration of the two active compounds are P - anisaldehyde and Linalool), especially when the spray of 50 mg. L - 1 of gibberellic acid and 1 mg. L - 1 of brassinolide, while the superiority of the holand breed Local product by giving it the highest concentration of other active compounds which four : (Anethol , Limonene , Carvone and Dillapiole), especially at a spray of 50 mg. L - 1 of gibberellic acid. and 2 mg. L - 1 of brassinolide.

دراسة كيمياوية وبايولوجية لبعض مركبات الايض الثانوي المستخلص من اوراق نبات البشملة L. Eroibotrya japonica المستزرع في العراق == Chemical and Biological Study of Some Secondary Metabolism Compounds Extracted from Bashmala Eriobotrya Japonica L. leaves Cultivated in Iraq

Author name: نعم حيدر جواد سعيد الجوهري
Supervisor name: بشرى محمد جابر علوش
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: This study containes the extraction and detection some of active compounds in the leaves of Eriobotrya japonica L. and testing the activity of crude extract as an antibacterial and food preservation. Methnol was used in crude extraction and also CC , TLC ,HPLC techniques were used in qultitive and quantative amount of some flavonoid ,terprnoid and phenolic compound .The well in agar to study the effect of crude on Staphylococcus aurus , Escherichia coli , Salmonella typhmurium bacteria in 200 ,100, 50 and 25 mg /ml concentrations , In addition to study of effect of the extraction on meat at 200 mg /ml concentration . The ruselts show that Alcholic crude extract contains flavonoid ,terprnoid and phenolic compounds and the chlororform was the best solvent in separating those compounds . Through the use TLC and HPLC , it was possible to separate terpenoids (Oleanolic acid , ursolic acid , maslinic acid) , flavonoids (Qurcetin, Kaempferol ,Rutine ) and phenolic compounds (Benzoic acid ,caffeic acid ,chlorogenic acid). The results show the high activity of leaves crude extract of Eriobotrya japonica L . in 200 mg /ml concentrationthat is used as a food preservation in minced beef meat. The results also show significant differences at (P<0.05) in Thiobarbutric acid ,Total violate nitrogen measurement ,total bacterial account , and psychrotrophic bacteria account ,AS for the value of PH was governor compared with standard control. The concentrations of cruds extract showed an Antibacterial activity where Staphylococcus aureus where 15 and 26 mm by using 100 and 200 mg/ ml concetrations respectively .furthermore,The crude extractshowed an efficiency as antibacterial agains Escherichia coli with 13and 15mm inhabition zone ; and also Salmonella typhmurium with 14 and 19mm inhabition zone by using 100 and 200 mg / ml concentration , respectively compared with standard control . The ruselts show that minimum inhibitory concentration of the cruds extract agains Staphylococcus aureus , Escherichia coli , Salmonella typhmurium bacteria was 100 mg / ml compared with standard control .

دراسة كيميائية وبايولوجية لمركبي Stigmasterol و? - sitosterol كمكونات فعالة متواجدة في نبات البنفسج العطري (العائلة : البنفسجية) المزروع في العراق == Phytochemical and Biological Study of Stigmasterol and ? - sitosterol as Active Constituents Present in Viola odorata L.Plant (Family : Violaceae) Cultivated in Iraq

Author name: شيماء فخري جاسم السالم
Supervisor name: بشرى محمد جابر علوش
General topic: Biology
Specific topic: Plant
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: تعد هذه الدراسة مسح لبعض المكونات الفعاله لنبات البنفسج العطري Viola odorata L. وعزل هذه المركبات الفعالة (Stigmasterol and β - sitosterol)، كما تم اجراء دراسه بايولوجيه لتحديد فاعليتها كمركبات مضادة للاكسدة وكمضادات بكتيرية حيث تضمنت الدراسه مايلي : - • تم استخلاص هذه المركبات كمستخلص خام من كل جزء من اجزاء النبتة الجذور،الاوراق, الازهار والبذور باستخدام الميثانول والبتروليوم ايثر واجراءالتقدير الكمي والنوعي للمركبين Stigmasterol) و( β - sitosterolفي كل جزء باستخدام تقنيات TLC, HPLC,CC• تم استخلاص وعزل وتشخيص المركبين (Stigmasterol and β - sitosterol) وذلك باستخدام طرق استخلاص وتجزئه وعزل مختلفه• تم استخدام المستخلص الخام لنبات البنفسج العطري Viola odorata L. والمركبات الستيرويدية Stigmasterol) و( β - sitosterol كمضادات للاكسدة على المركب 1 - diphenyl - 2 - picrylhydrazyl (DPPH) الفعال لاحتوائه على الجذور الحرة وتحويله الى مركب 1,1 - diphenyl - 2 - picryl - hydrazine باستخدام التراكيز 25, 50, 100, 200 و400 مايكروغرام/مليلتر والمقارنة مع حامض الاسكوربك كمعاملة قياسية• تم استخدام المستخلص الخام لنبات البنفسج العطري Viola odorata Lو المركبات الستيرويديه( Stigmasterol و( β - sitosterol كمضادات بكتيرية فاعلة ضد بكتيريا staphylococcus aureus وPseudomonas aeruginosa وبتراكيز 2, 5, 10, 20, 30, 40 و50 مايكروغرام/مليلتر والمقارنة مع المضاد الحيوي Gentamicin كمعاملة قياسية• اظهرت النتائج وجود كميات كبيرة من المركبات الستيرويدية(Stigmasterol and β - sitosterol )في كل اجزاء نبتة البنفسج العطري Viola odorata L. اذ كانت تراكيز مركب Stigmasterol في الاوراق 2 ملغم/100مليلتر وللازهار 7.3 ملغم/100مليلتر وللجذر 4.5 ملغم/100مليلتر وللبذور 2.4 ملغم/100مليلتر, فيما كانت تراكيز المركب β - sitosterol في الاوراق 3.2 ملغم/100مليلتر وللازهار 5.3 ملغم/100مليلتر وللجذر 9.2 ملغم/100مليلتر وللبذور 3 ملغم/100مليلتر, اذ كان اعلى تركيز للمركب Stigmasterol في مستخلص الازهار 7.3 ملغم/100مليلتر واعلى تركيز للمركب β - sitosterol في الجذر 9.2 ملغم/100مليلتر.• اظهرت النتائج الفاعلية الكبيرة للمستخلص الخام لنبات Viola odorata L. بالتركيز 400 مايكروغرام/مليلتر كمضاد للاكسدة بنسبة 94% وللتراكيز 25, 50, 100 و200 مايكروغرام/مليلتر بنسبة 72, 80, 84 و92% على التوالي بالمقارنة مع المعاملة القياسية, اذ اظهرت النتائج فروقا معنوية P≤ 0.05 ما بين التراكيز للمستخلص الخام, فيما اظهر التركيز 400 مايكروغرام/مليلتر لخليط المركبات( stigmasterol وβ - sitosterol) كفاءة كبيرة كمضادات للاكسدة وبنسبة 74% وللتراكيز 25, 50, 100 و200 مايكروغرام/مليلتر بنسبة 59, 63, 66 و70% على التوالي مقارنة بالمعاملة القياسية وبفروق معنوية P≤0.05 ما بين التراكيز.• اظهرت تراكيز المستخلص الخام للنبتة فاعلية كمضاد للبكتيريا اذ كان مدى تثبيط بكتيريا Staphylococcus aureus على الوسط المغذي الصلب 0, 5, 15, 18, 20, 22 و25 مليمتر باستخدام التراكيز 2, 5, 10, 20, 30, 40 و50 مايكروغرام/مليلتر على التوالي, واظهر خليط الستيرويدات مدى تثبيط 0, 0, 0, 14, 16, 19 و21 مليمتر لنفس التراكيز على التوالي ومقارنة بالمعاملة القياسية, كذلك اظهر المستخلص الخام كفاءة كمضاد بكتيري ضد بكتيريا Pseudomonas aeruginosa بمدى تثبيط 0, 5, 12, 15, 17, 19 و22 مليمتر ولخليط الستيرويدات بمدى تثبيط 0, 0, 0, 11, 13, 17 و18 مليمتر مقارنة بالمعاملة القياسية, وبينت النتائج ان التركيز الادنى المثبط للمستخلص الخام للنبتة ضد بكتيريا Staphylococcus aureus وPseudomonas aeruginosaهو 5 مايكروغرام/مليلتر ولخليط الستيرويدات هو 20 مايكروغرام/مليلتر. | This study is considered as a survey of the some secondary metabolits present in Viola odorata L., collected from plant nursery in Baghdad between April and July, followed by the fractionation and isolation of Stigmasterol and β - sitosterol.The biological study demonstrated the effect of the Stigmasterol and β - sitosterol found in Viola odorata L. as antioxidant and antibacterial. This study involved the following : - • Extraction of Stigmasterol and β - sitosterol as a crude extract from each part of the plant roots, leaves, flowers and seeds using (Petrolium ether and Methanol) to qualitative and quantitative estimation of Stigmasterol and β - sitosterol in each part of the plant by using TLC, HPLC and CC. • Fractionation and Isolation of sterols compounds (Stigmasterol and β - sitosterol) present in Viola odorata L., by using different methods . • Crude extract and sterols compound (Stigmasterol and β - sitosterol) has been studies in experimental work to determination the antioxidant activity against1,1 - diphenyl - 2 - picrylhydrazyl (DPPH) which is considered as stable free radical and convert it into 1,1 - diphenyl - 2 - picryl - hydrazine ,by using different concentrations (25,50,100,200 and 400µg/ml). Compare ascorbic acid.• Crude extract and sterols compound (Stigmasterol and β - sitosterol) has been used to determination the antibacterial activity using different concentrations (2, 5, 10,20,30,40 and 50µg/ml) against staphylococcus aureus and pseudomonas aeruginosa by using agar well diffusion method compare with Gentamicin as a standard control. • The phytochemical results showed that all the plant parts contain high amount of sterols, the quantitative data revealed that the total content of Stigmasterol in roots(4.5mg/100g) ,in leaves(2mg/100g),in flowers(7.3mg/100g) and in seeds(2.4mg/100g).The content of β - sitosterol in roots(9.2mg/100g),in leaves(3.2mg/100g),in flowers(5.3mg/100g) and in seeds(3mg/100g), highest amount of stigmasterol founded in flowers (7.30mg/100g) ,the maximum amount of β - sitosterol in roots (9.2 mg/100g) .• The results showed that crude extract of Viola odorata in concentration of 400µg/ml have a higher antioxidant effect by 94%, and concentrations of 25, 50, 100 and 200 µg/ml by 72, 80, 84 and 92% respectively which are comparing with standard control Ascorbic acid, the results showed there are significant difference at (P≤ 0.05) between the concentrations in the same crude extract, while the stigmasterol and β - sitosterol of Viola odorata also showed higher antioxidant effect in concentration of 400µg/ml by 74% and concentrations of 25, 50, 100 and 200µg/ml by 59, 63, 66 and 70% respectively comparing with standard control Ascorbic acid with significant difference at (P≤0.05) between them ,and with significant difference at (P≤0.05)between the concentrations in the same fraction.• Antibacterial results of crude extract showed Staphylococcus aureus growth inhibition zone by 0, 5, 15, 18, 20, 22 and 25 mm with 5 µg/ml as MIC, while, sterols compound showed inhibition zone by 0, 0, 0, 14, 16, 19 and 21 mm with 20 µg/ml as MIC comparing with Gentamicine standard control. Furthermore inhibition zone in pseudomonas aeruginosa culture when using crude extract by 0, 5, 12, 15, 17, 19 and 22 mm with 5 µg/ml as minimum inhibitory concentrations (MIC), while, sterols compound showed inhibition zone by 0, 0, 0, 11, 13, 17 and 18 mm with 20 µg/ml as Minimum inhibitory concentration (MIC) compar with Gentamicin standard control .Stigmasterol and β - sitosterol compound showed inhibition zone by 0,0,0,11,13,17 and 18 mm with 20 µg/ml MICcompared with Gentamicine standard control.

استخلاص وتنقية وتشخيص زيت نبات الرازقي المستزرع في العراق L. Jasminum sambac ودراسة تاثيره كعامل مضاد للاكسدة == Extraction, Purification and Identification of Jasmine oil in Jasminum sambac L. Cultivated in Iraq and It's effect as antioxidant activity

Author name: زينب عمران سلمان الربيعي
Supervisor name: بشرى محمد جابر علوش
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: هدفت هذه الدراسة الى استخلاص زيت الياسمين من ازهار واوراق وسيقان وجذور نبات الرازقي المستزرع في العراق Jasminumsambac L., والكشف عن المركبات الموجودة فيه, كما تم اجراء مقارنة بين الزيت الطيار والمستخلص الكحولي الخام للنبات كمركبات مضادة للاكسدة باستعمال الجذرالحر(1 - diphenyl - 2 - picryl - hydrazyle) ((DPPH وذلك باستعمال التراكيز (100,80,60,40,20)l/mlµ والمقارنة مع حامض الاسكوربك كمعاملة قياسية. استخلص الزيت الطيار باستعمال طريقة التقطير بالبخار والاستخلاص بالكحول بواسطة جهاز الكلافنجرClavenger apparatus) ), كما تم التعرف على مكونات الزيت الطيار بتقنية كرموتوغرافيا الغاز السائل - مطياف الكتلة. اجريت عملية الاستخلاص الكحولي (الخام) للمركبات الفعالة من النبات بجهاز(Soxhlet), وتم الكشف النوعي عن المركبات الفعالة بواسطة عدد من الكواشف, وبينت النتائج ان المستخلص الكحولي لكلا الجزئين احتوى على مركبات فعالة (القلويدات, الفلافونيدات, التربينات, الصابونيات, التانينات). اشارت النتائج الى ان استخلاص 100غم من نبات الرازقي لكل من الازهار والاوراق انتجت زيتا طيارا 5%, 1.5 % على التوالي, اما السيقان فكانت كمية الزيت الطيار فيها قليلة جدا بحيث كان من الصعب جمعها. اما بالنسبة لجذور النبات فلم يظهر وجود للزيت الطيار فيها. اثبتت الدراسة ان الزيت الطيار للازهار يحتوي على (30 مركبا ) وكان اعلى تركيز لمركب Benzyl acetate حيث بلغت نسبته المئوية 59.37%, يليه مركب Benzyl alcohol بتركيز 14.96%, ثم مركب Benzyl propanoate بتركيز 5.25%. اما زيت الاوراق فاظهرت النتائج انه يحتوي على 43 مركبا وان مركب Benzyl acetate كان الاعلى تركيزا وبنسبة 34.07% , يليه مركب N - carbobenzooxy arginine بتركيز 15.28% , ثم مركب benzyl propanoate بتركيز7.79%.كما اظهرت النتائج الفعالية العالية للزيت الطيار لكل من الازهار والاوراق بالتركيز 100 l/mlµ كمضادا للاكسدة وبنسبة 96.10%, 82.30% على التوالي بالمقارنة مع المعاملة القياسية (حامض الاسكوربك), اذ اظهرت النتائج فروقا معنوية مابين تراكيز الزيت الطيار لكل من الازهار والاوراق. اما فيما يخص نتائج المقارنة بين الزيت الطيار والمستخلص الخام للنبات فقد تبين ان الفعالية المضادة للاكسدة للزيت الطيار والمستخلص الكحولي لللازهار عند تركيز 100 l/mlµ للزيت الطيار وg/mlµ للمستخلص الخام كانت 96.10% , 33.87% على التوالي, اما بالنسبة للزيت الطيار والمستخلص الكحولي للاوراق عند نفس التركيز فكانت النسبة المئوية المضادة للاكسدة 82.30%, 22.08% على التوالي وبفروق معنوية مابين تراكيز الزيت الطيار والمستخلص الكحولي. | This study aimed was designes to extract Jasmine oil from flowers, leaves, stems, and roots using Jasminum sambac L. which cultivated in Iraq, and detecting the existing compounds. A comparison was conducted between volatile oil and alcoholic extract ( as a crude) of the plant as antioxidant by using the compound 1 - diphenyl - 2 - picryl - hydrazyle (DPPH) as a free radical at the concentrations 20,40,60,80,100 Microliter/ milliliter then compared with ascorbic as a standard treatment. The volatile oil was extracted by using the steam distillation and alcohol extract using Clevenger apparatus. volatile oils were detected by using the chromatography of the liquid gas. In addition, alcoholic crude extract was done by using Soxhlet. The qualitative detection was conducted searching for active compounds through the use of a number of detections reagents.The results of the qualitative detection showed that the alcoholic extract in both plant parts contains the active compounds alkaloids, flavonoids, terpenoids, saponins, tannins. The results showed that 100 g of flowers and leaves has produced a volatile oil estimated by 5% and 1. 5% respectively. While the amount of the volatile oil in the stems was very little to be extracted and thus it was difficult to collect. No volatile oil was detected in plant roots. The study has shown that volatile oil of flowers contains 30compounds and the highest was Benzyl acetate, reaching 59.37%, followed by Benzyl alcohol (14.96%), then Benzyl propanoate which recorded 5.25%. The study showed that volatile oil in leaves contains 43compounds and Benzyl acetateeas represented the highest (34.07%), followed by N - carbobenzooxy arginine (15.28%), then benzyl propanoate (17.79%). The results also showed high volatile oil antioxidant activity in both flowers and leaves at the concentration 100Microliter / ml reached 96.10% and 82.30% respectively compared with Ascorbic Acid. the results also indicate significant differences between the volatile oil concentrations in both flowers and leaves. It has been proved that the maximum antioxidants activity for volatile oils and alcoholic extract of flowers was at a concentration of 100Microliter / ml was reached 96.10%, 33.87% respectively, while for volatile oil and alcoholic extract of the leaves, the percentages were 82.30% and 22.08% respectively, with significant differences between volatile oil and alcoholic extract concentrations.

المعالجة النباتية لعنصري الرصاص والنحاس باستعمال نبات عصا الراعي Polygonum sp.مختبريا == Phytoremediation of lead and copper elements a by polygonum sp. Plant in Laboratory

Author name: رواء حسان محمد عبد الله
Supervisor name: عبد الرحمن عبد الجبار الكبيسي
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت هذه الدراسة للتعرف على دور نبات عصا الراعيPolygonum sp. في ازالة بعض العناصر الثقيلة من المياه ، وقد استعملت تراكيز مختلفة من عنصري النحاس والرصاص بصورة منفردة وهي ( 10 ، 20 ، 30 ) ppm ، ولمدة تسعة ايام لكل تركيز, وقدرت كفاءة النبات في ازالة العناصر الثقيلة من المياه كنسب مئوية للازالة في الساق والاوراق. بينت النتائج ان النبات له القابلية على تراكم عنصري الرصاص والنحاس في الساق والاوراق بكميات كبيرة. وكان للاوراق القابلية على تراكم العناصر الثقيلة اكثر مما هي عليه في الساق حيث ان الورقة هي المكان الاخير الذي تنتقل اليه العناصر . وجدت فروق معنوية لكل من الاوراق والساق لكلا العنصرين عند مستوى احتمالية P≤0.05، فقد وجدت فروق معنوية بين العنصرين في اغلب التراكيز المدروسة خلال مدة التجربة. اظهر النبات نسب ازالة مئوية مرتفعة لعنصر النحاس وكانت بين (32.02 - 87.01) % وهي كالاتي : - 49.35) ،80.97،87.01)% للتراكيز المدروسة بعد مرور تسعة ايام على التجربة على التوالي ، بينما تراوحت نسب ازالة عنصر الرصاص بين 71.41)%97.85 - ) % وهي كالاتي : (97.85 ، 96.71 ، 92.93 ) % للتراكيز المدروسة على التوالي بعد مرور تسعة ايام من التجربة ، وتم قياس نسبة تركيز العناصر بعد (6 - 3 و9) ايام من التجربة . بينت النتائج ان النسبة المئوية لازالة عنصر الرصاص اعلى من النسبة المئوية لازالة عنصر النحاس ،لكن عنصر النحاس تاثيراته المظهرية على النبات اسرع من عنصر الرصاص، اذ بدات الاوراق بالذبول عند المعاملة بعنصر النحاس اسرع مما هو عليه عند المعاملة بالرصاص . | The present study was carried out to determine the ability of Polygonum SP. for removing of heavy element from water. Different concentrations of Copper and Lead (10, 20 and30) ppm were used for nine days to each concentration. The plant efficiency in removing heavy element from water was estimated as percentage removal ratios in stem and leaves. Results were showed that the plant can accumulate copper and lead in stem and leaves in large amounts. leaves ability to accumulate heavy element was more than stem. Significant differences were found between stem and leaves for both elements in probability level P≤0.05, as well as between both elements for almost concentrations during experiments. Polygonum sp. showed high removal percent ratio of Copper which were ranged between 32.02 - 87.01% as follows : 49.35 , 80.97 and 87.01 for the studied concentrations, respectively, after nine days. While the removal ratios of Lead were ranged between 97.85 - 71.41 as follows : 97.85, 96.71, and 92.93 for studied concentrations after (3,6,9) day. Results were showed the percentage removal to Lead more than Copper,but Copper was more affect on morphology for plant than Lead, the leaves start shriveling when they were treated with Copper and faster than those treated with Lead.

دراسة تصنيفية حياتية لانواع معينة من الجنس Astragalus L. من العائلة البقولية Leguminosae في العراق == Biosystematic study of certain species of the genus Astragalus L. from the family Leguminosae in Iraq

Author name: خنساء رشيد مجيد
Supervisor name: خليل ابراهيم الشمري
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: هدفت الدراسة الحالیة الى تصنیف انواع من الجنس Astragalus L. من خلال دراسة الصفات المظهریة والتشریحیة والكیمیائیة والوراثية والتوزيع الجغرافي والتصنيف العددي لستة قطاعات متكاملة والحاوية عشرة انواع جمعت من مقاطعات وسط وشمال وجنوب العراق، والقطاعات والانواع التي درست هي : Sec. Aulacolobus : A. guttatus Banks & Sol. ; Sec. Buceras : A. campylorrhynchus Fisch. & Mey. and A. hamosus L.; Sec. Harpilobus : A. corrugatus Bertol. and A. hauarensis Boiss.; Sec. Hololeuce : A. alyssoides Lam.; Sec. Malacothrix : A. mollis M. Bieb. , A. pulchellus Boiss. and A. sarae Eig.; Sec. Sewerzowia : A. oxyglottis Stev. تضمنت الدراسة التعرف على الصفات المظهریة للجذور والسیقان والاوراق والنورات الزهریة والازهار والثمار والبذو، اما في الجانب التشریحي فتم دراسة التغايرات بین الانواع المدروسة على مستوى السیقان والاوراق والسويق) البشرة والقشرة، عددها وسمكها واعداد الحزم الوعائیة وترتیبها واشكالها ( والمعقدات الثغریة ونمط التعرق اذ كان من نوع Brochidodromous في جميع الانواع المدروسة، كم درست اشكال حبوب اللقاح وبینت نتائج الدراسة الحالیة ان هناك امكانیة للفصل بین الانواع المدروسة باستعمال بعض الصفات المظهریة الدقیقة لحبوب اللقاح اذ كانت حبوب اللقاح من الطراز ثلاثي الاخادید المثقبة Tricolporate في جمیع الانواع المدروسة، تضمنت الدراسة كذلك التعرف ولاول مرة باستعمال تقنیة كروموتوكرافيا الطور السائل عالي الاداء HPLC (High performance liquid chromatography) على محتوى الاوراق من الفلافونویدات لانواع الجنس المدروسة، اذ تم تشخیص ثمانية مركبات فلافونویدیة وهي : myrectin، quercetin، rahmnocitrin، isorhamnetin، kaempherol، formononetin، rutin، apigenin مع تحديد تراكيزها، فضلا عن ذلك شملت الدراسة الجانب البیئي، وقد وزعت الانواع جغرافيا على مناطق انتشارها وفي بیئاتها المختلفة ورسمت خرائط توزیع الانواع على المقاطعات الجغرافية في المناطق الجنوبية والوسطى والشمالیة. | The present study aimed to consider the morphological, anatomical, chemical and genetic characters and geographical distripution of six sections and ten species belong to the genus Astragalus L. In the central, northern and south part of Iraq, the sections and species were : Sec. Aulacolobus : A. guttatus; Sec. Buceras : A. campylorrhynchus and A. hamosus; Sec. Harpilobus : A. corrugatus and A. hauarensis; Sec. Hololeuce : A. alyssoides; Sec. Malacothrix : A. mollis, A. pulchellus and A. sarae; Sec. Sewerzowia : A. oxyglottis. The research includes study of morphological characteristics of the roots, stems, leaves, flowers inflorescences, fruits and seeds, In addition anatomical study that consider variations between the species studied at the stem, leaves and petiole level (epidermis and cortx the thickness, the nature and arrangement of tissues and the number of vascular bundles, their arrangment and forms), epidermis stomatal complexes, venation patterns which they were Brochidodromous in all species in this study and the form of pollen grain which has been Tricolporate in all species in this study. The study also identified for the first time using a technique chromotochraph liquid phase HPLC on the content of the leaves of the flavonoids of the plants studied were diagnosed ten flavonoad compound in the leaves of the plants such as myrectin، quercetin، rahmnocitrin، isorhamnetin، kaempherol، formononetin، rutin، apigenin as well as the study included a study of the environmental side, has distributed species geographically "on areas of in environments different and mapped the distribution of species on geographical districts in the central and northern and south regions. Maps of geographical distribution were prepared for all species studied. The study shown that the species were distributed in the north, east and west area of Iraq.

دراسة تشريحية مقارنة لتراكم الصابونيات وتقديرها في نبات Yucca gloriosa Variegata L. الكامل ومزارعه النسيجية

Author name: انسام غازي عبد الحليم علي
Supervisor name: بشرى محمد جابر علوش | كاظم محمد ابراهيم
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: يعد نبات الخنجر الاسباني Yucca gloriosa Variegata L. نبات زينة وطبي مهم, ينمو في المناطق الجافة وشبه الجافة حول العالم. يعد التكاثر الخضري التقليدي للنبات عن طريق العقل والخلفات وسيلة غير كافية نظرا الى الاعداد المحدودة التي يستطيع انتاجها، وعليه توفر تقنية زراعة الانسجة النباتية طريقة بديلة للانتاج التجاري لهذا النبات. ازداد الاهتمام بنبات Y. gloriosa لان جميع اجزاء النبات غنية بالمركبات الستيرويدية التي لها قيمة علاجية واسعة. اجريت الدراسة بهدف اكثار هذا النبات نسيجيا واختبار انتاج الانسجة من الصابونيات الستيرويدية في انسجة الزروعات ومقارنتها مع محتوى هذه المركبات في النبات الكامل باستعمال التحليلات الكروماتوغرافية والمجهر الفلوريسيني والذي يعتبر طريقة جديدة فعالة لتقدير مستوى تراكم المركبات الثانوية واختبار المنتجات النباتية الطبيعية التي تستعمل كعقاقير طبية. لدراسة الاخلاف خارج الجسم الحي اضيفت تراكيز مختلفة من منظمات النمو البنزل ادنين BA)) وحامض النفثالين NAA)) وتداخلهما الى الوسط الغذائي موراشيج وسكوج(MS) لاستحثاث الكالس من الاوراق والبراعم. سجلت النسبة المئوية لاستحثاث الكالس والوزن الطري ولون وبنية الكالس بعد 1.5 و6.0 اشهر من الزراعة. استحث الكالس من اجزاء الاوراق في وسط MS مجهز ﺑ 6 ملغم/لتر NAA. اظهرالتداخل بين 0.2 ملغم/لتر BA و1.5 ملغم/لتر NAA تاثير ملحوظ في نشوء الكالس من البراعم. تم اختبار تاثير اضافة منظم النمو الثايدايازورون (TDZ) ﺑتراكيز مختلفة (0.0, 0.1, 0.5 او (1.0 ملغم/لتر الى وسط MS مجهز ﺑ BAو NAAفي استحثاث الكالس. سجل اسرع نمو واكثر انتاجية للكالس من البراعم في الوسط المجهز ﺑ 0.1 ملغم/لتر BA مع 1.5 ملغم/لتر NAA و1.0 ملغم/لتر TDZ. اظهر TDZ تاثيرا ايجابيا في ٳستحثاث الكالس من البراعم مقارنة مع BA او NAA, واعطت بعض التداخلات تكوين مباشر للافرع الخضرية. تميز الكالس الناتج من البراعم بلونه الٲبيض المائل الى الكريمي وبكونه متراص, بينما كان كالس الاوراق اخضر اللون ومفصص. تم اختبار تاثير عدة تداخلات من BA 3)، 6، 7، 8 او 9 ملغم / لتر) و0.05) NAA، 0.1 او 0.2 ملغم / لتر) في تكوين النبيتات على وسط MS في غضون 12 اسبوعا، كان اعلى تكوين للنبيتات بوجود 9) ملغم / لتر) BA و0.1) ملغم / لتر) NAA . حدد تاثير اندول حمض البيوتريك (IBA) بتراكيز مختلفة في تكوين الجذور. ان افضل تجذيرللنبيتات كان في وسط MS بقوة ½ المجهز ﺑ 1.0 ملغم / لتر IBA. استخلصت وشخصت الصابونيات الستيرودية التايكوجينين والجيتوجينين من اجزاء النبات الكامل (اوراق، جذور وقمم نامية) ومزارعه النسيجية (الكالس، الاجزاء الخضرية التي تم اخلافها بطريقة مباشرة وغير مباشرة، الجذور) من النبات. جرى الكشف الاولي للصابونيات الستيرويدية باستعمال الطبقة الرقيقة الكروماتوغرافية (TLC). كما قدرت الصابونيات الستيرويدية (التايكوجينين والجيتوجينين) باستعمال الكروماتوغرافي عالي الاداء (HPLC). اظهرت مستخلصات الكحول البيوتانولي وجود التايكوجينين والجيتوجينين في كل جزء من النبات ومزارعه النسيجية بمدى يتراوح من 128.29الى409.75 ومن51.04 الى236.62 مايكروغرام/غرام على التوالي. سجلت اعلى قيمة للسابوجينينات sapogenins في اوراق النبيتات المعاد اخلافها بطريقة غير مباشرة. ان كمية هذه المركبات كانت جديرة بالاعتبار في انسجة الكالس المعامل مع TDZ اعتمادا على فترة نشوء الكالس القصيرة (ثلاثة اسابيع). نفذت دراسة تشريحية لملاحظة تراكم الصابونينات في اجزاء النبات الكامل ومزارعه النسيجية باستعمال المجهر الفلوريسيني وتبين بان اعلى تراكم قد ظهر في انسجة اوراق النبيتات التي تم اخلافها بطريقة غير مباشرة وفي انسجة الكالس المستحث نشوءه بمنظم النمو .TDZ سجل تغير في محتوى الصابونيات خلال فترات نمو الكالس المختلفة. | Yucca gloriosa Variegata L. is an important ornamental and medicinal plant, grows in arid and semi - arid regions around the world. Traditional vegetative propagation of the plant by cuttings and offsets is not adequate due to the limited numbers that can be produced. Plant tissue culture technique provides an alternative method for commercial propagation of this plant. The interest in Y. gloriosa increased because all parts of this plant are rich in steroidal compounds that have vast therapeutic values. This study was performed to propagate this plant in vitro and investigate the yield of steroidal saponins in the plant tissue cultures. Comparison of the saponins yield in intact plant and tissues or organs derived from it using chromatographic analysis and fluorescence microscopy was carried out. To search the potential of in vitro rapid regeneration of this plant, different concentrations of 6 - Benzyladenine (BA), 1 - naphthaleneacetic acid (NAA) and combinations of both were assessed for callus induction on leaf and bud explants using Murashige and Skoog (MS) medium. Callus response initiation percentage, fresh weight, texture and color of the callus were evaluated after 1.5 and 6.0 months in culture. The appropriate medium for callus initiation on leaf explants was MS medium supplemented with 6.0 mg/l NAA. A combination of 0.2 mg/l BA and 1.5 mg/l NAA also exhibited a remarkable callus induction on bud explants. Addition of thidiazuron (TDZ), BA and NAA at various concentrations (0.0, 0.1, 0.5 or 1.0 mg/l) were examined for callus initiation. Faster growth and more proliferation of callus on bud explants were observed on MS medium supplemented with 0.1 mg/l BA + 1.5 mg/l NAA + 1 mg/l TDZ. Results showed that TDZ was more efficient for inducing callus than BA or NAA however some combinations gave direct shoot regeneration. Calli derived from bud explants were whitish to creamy and compact, while calli derived from leaf explants were greenish. The influence of different combinations of BA (3, 6, 7, 8 or 9 mg/l) and NAA (0.05, 0.1 or 0.2 mg/l) on shoot proliferation on MS medium were tested. Within 12 weeks, the highest shoot formation was in the presence of BA (9 mg/l) and NAA (0.1 mg/l). The effect of indole - 3 - butyric acid (IBA) at different concentrations on root proliferation was determined. The best rooting of plant microshoots was found in MS medium provided with 1.0 mg/l IBA. The extraction and identification of steroidal sapogenin tigogenin and gitogenin from both intact plant parts (leaves, rhizomes and shoot apices) and tissue cultures (calli, direct and indirect regenerated shoots, rhizomes) of Y. gloriosa Variegata L. were performed. Preliminary identification of steroidal sapogenin was done using thin layer chromatography (TLC). Qualitative and quantitative assessment of steroidal saponins (tigogenin and gitogenin) was also performed by using high performance liquid chromatography (HPLC). The butanolic extracts showed the existence of tigogenin and gitogenin in all plant parts and their tissue cultures ranging from 128.29 to 409.75 and 51.04 to 236.62 μg/g respectively. Maximum sapogenins value was recorded in indirect regenerated shoots. The amount of these sapogenins in callus treated with TDZ was considerable since callus initiated rapidly within 3 weeks. Anatomical study investigating saponins accumulation in both intact plant parts and their tissue cultures were performed using fluorescence microscopy under ultraviolet (UV) to determine the site of saponin accumulation. Histological analysis using fluorescence microscope showed that saponins primarily accumulated in indirect regenerated leaf tissues and in callus treated with TDZ. Saponins levels changed during different periods of callus growth

دراسة تشريحية مقارنة لانواع مختارة من العائلــــــــة المركبــــة Compositae الناميـــــة ضمن مجمع الجادريــــــة ـ جامعـــة بغداد == Comparative anatomical study of selected compositae species grown in Jadiriyah Campus , Baghdad University

Author name: سكينة عباس عليوي
Supervisor name: علي حسيـن عيسى الموسوي
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: تناول البحث الحالي دراسة تشريحية مقارنة لانواع عشرة مختارة من العائلة المركبة Compositae النامية في مجمع الجادرية - جامعة بغداد ، والانواع هي : Aster subulatus Michx.Calendula officinalis L.Calendula Persica C. A. MayCarduus pycnocephalus L.Carthamus oxycantha M. B.Eclipta alba (L.) HaussknErigeron canadensis L.Silybum marianum (L.) GarthUrospermum picroides (L.) Schmidt .Xanthium brasilicum Vellozo .حيث ركز البحث على بعض الصفات التشريحية ودراستها بصورة مقارنة ولاول مرة لهذه الانواع في العراق. وتضمنت الدراسة مميزات البشرة مثل دراسة خلايا البشرة الاعتيادية والمعقدات الثغرية. واسفرت الدراسة عن ظهور طراز جديد ولاول مرة في هذه الانواع وهو الطراز المتوازيParacytic type للنوع Eclipta alba . كما تمت دراسة نظام التعرق للانواع المذكورة انفا ومقارنتها وكذلك الخصائص التشريحية للورقة واظهرت المقاطع العمودية لنصل الورقة لاسيما شكل الحزم وعددها وسمك الطبقتين العمادية والاسفنجية وبقية الصفات حيث اعطت خصائص تصنيفية مهمة ، وامكن تقسيم الانواع على مجاميع ، وبينت الدراسة للمقاطع المستعرضة للسيقان خاصة صفات تصنيفية مهمة. ومميزة من حيث : سمك الكيوتكل وسمك طبقة البشرة ووجود الكولنكيما او عدم وجودها ، وعدد صفوفها ونوعها . وكان لصفات الحزمة الوعائية اهمية مميزة من حيث معدل طولها واعدادها واشكالها مما امكن تقسيم الانواع على مجاميع ، كذلك درس اللحاء والخشب حيث ابديا اهمية تصنيفية من جوانب عدة ، وامكن الاستفادة من الاسطوانة الوعائية في عزل الانواع سواء اكانت مستمرة ام متقطعة على شكل حزم كذلك درس اللب واعطيت له اهمية من حيث المساحة ووجود او عدم وجود البلورات وانواعها ان وجدت . وتضمن البحث دراسة المقاطع المستعرضة للجذور ، واظهر سمك البشرة والقشرة واللحاء وشكله ومساحة منطقة الخشب اهمية كبيرة في العزل والتشخيص وكان للكساء السطحي للاوراق والسيقان فائدة وقيمة تصنيفية مهمة في عزل الانواع من حيث اشكال الشعيرات واطوالها وانواعها ومن حيث وجود او عدم وجود الحليمات . واسفرت الدراسة عن ظهور نتائج وتغايرات وصفات مهمة تعرض لاول مرة ، لقد اعطت الدراسة التشريحية صفات مهمة اعطت دعما واسنادا للصفات المظهرية وساعدت على عزل وتشخيص الانواع ضمن العائلة نفسها ، هذا واورد البحث صورا للعينات الحقلية التي جمعت من مواقع مختلفة ومتعددة ضمن مجمع الجادرية ( رقعة الدراسة ) ، وتضمن صورا عديدة للمقاطع التي تمت دراستها للاعضاء النباتية المختلفة فضلا عن العديد من الجداول . وقد نوقشت النتائج لهذه الدراسة التشريحية خصوصا ما هو متعلق ببيئة هذه الانواع . | The present study dealt with ten selected species of the family compositae grown in Jadiriyah campus , university of Baghdad , were studied anatomically in a comparative way within this work . These species are : Aster subulatus Michx.Calendula officinalis L.Calendula Persica C. A. MayCarduus pycnocephalus L.Carthamus oxycantha M. B.Eclipta alba (L.) HaussknErigeron Canadensis L.Silybum marianum (L.) GarthUrospermum picroides (L.) Schmidt .Xanthium brasilicum Vellozo .Comparative anatomical characters were used for the first time in these species grown in Iraq , such as characteristics of ordinary epidermal cells and stomatal complexes . Paralytic type was recorded as new founding in these species namely in Eclipta alba . Venation system was investigated and compared , Vertical sections of leaf blades , especially the shape and number of vascular bundles , thickness of palisade of spongy layers and other mesophyll characters . These characters were important taxonomically ,according to these species were divided into groups based on these specific characters . Cross sections of stems show important taxonomic values , like thickness of cuticle , epidermis , presence or absence of collenchyma , types and rows of this collenchyma . Characteristics of the vascular bundle were important too , therefore the species were divided according to these attributes into groups. Xyelem and phloem were also used taxonomically . The vascular cylinder was useful whether it was continuous as a complete cylinder or as a separated strands.Pith of the stem and its size , presence of crystals was of a considerable importance . Other characters of the plant organs , cross sections of root were useful taxonomically , especially the thickness of epidermis , cortex , phloem and xylem .Indumentum of leaves and stems was also of a taxonomic value , therefore the shapes , length , kinds , presence of glands were used for identification of the species . This study concluded the presences of variation in characters ; and these were presented for the first time comparatively , so that the anatomical characters were of a good supporting to the exomorphological characters in the family compositae . Graphes , field photographs of the different plants and sections of plant organs were put in addition to a numerous tables in this work . The above results were discussed scientifically regarding the invironmental factors .

تاثير بعض المحفزات في انتاج بعض المركبات الثانوية لنبات الترنجان L. Melissa officinalis خارج الجسم الحي == Effect of Some Elicitors on Some Secondary Compounds Production of Melissa Officinalis L. In Vitro Culture

Author name: ميساء حامد احمد العبيدي
Supervisor name: لمياء خليفة جواد العامري
General topic: Agricultural sciences
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: تم تنفيذ هذه الدراسة في مختبر زراعة الانسجة النباتية التابع لقسم البستنة وهندسة الحدائق - كلية الزراعة - جامعة بغداد وجرت التحليلات في مختبر الحقول البيضاء، بهدف توظيف تقانة زراعة الانسجة النباتية في امكانية زيادة بعض المركبات الفينولية (Rosmarinic acid، | The plant tissue culture lab, of Horticulture dept. Abo Ghareeb - College of Agriculture - University of Baghdad, have witnessed a research work, which aims to make use of in vitro culture technology to evaluate the ability of increasingsome plant seconda

دراسة تصنيفية حياتية مقارنة لانواع من الجنس Euphorbia L. (Euphorbiaceae) في العراق == A Comparative Biosystematic Study of Some Species of The Genus Euphorbia L. (Euphorbiaceae) In Iraq

Author name: ازهار طاهر صليبي
Supervisor name: عذية ناهي سلمان المشهداني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: تناول البحث الحالي دراسة تصنيفية مقارنة لثلاث وعشرين مرتبة تصنيفية تعود جميعها للجنس Euphorbia L. من العائلةEuphorbiaceae النامية في العراق، تضمنت الجوانب المظهرية العامة والدقيقة ودراسة حبوب اللقاح والمركبات الكيميائية والبيئة والتوزيع الجغرافي فضلا عن ا | The current research deal with twenty - three taxa belong to the genus Euphorbia L., Family Euphorbiaceae grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also studied and the chemical compounds, Ecological and geographical distribution and taxonomic treatment have done.Also botanical key was putted to separate the species. General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens studied, and correct the mistake naming, specimens were identified and reidentified some miss identified specimens in the Iraqi herbaria. A comparative morphological study was undertaken to all plants parts of taxa appeared from habit and duration of taxa to the quantitative and qualitative attributes the members of the vegetative and floral parts included the roots, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying with natural images for different morphological characteristics, as well as the field images. This study showed that all species was herbs, shrubs and succulent and semisucculent annual and perinnal, the major taxonomic importance was for flowering parts includeing the specific Inflorescence, installation of a cupped and its regularity on the branches of the plant, glands forms, numbers, presence or lack of appendages, bractes and bracteoles shapes, dimensions and color fruits shape, indumentum and seeds forms, dimension and her surface sculpturing; and the presence or lack of caruncle, as well as the qualities of roots, stems and leaves, and the morphological characteristics of all plant parts mentioned above have a taxonomic importance in identification and separation of the studied taxa. The study of the micromorphological characters of hairs, leaves epidermal cells and the stomatal complex which included anomocytic, paracytic, anisocytic and tetracytic, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa.The pollen grains have a taxonomic importance because their different in shape, dimensions of polar and equatorial view, the wall thickness and their ornamentation. They are mostly of Tricolporate except two species were Hexacolporate, also the differences in their shape in the polar and equatorial view.They were spheroidal, ovoid and oblate - spheroidal in the polar view and oblate, spheroidal, subprolate and oblate - spheroidal in the equatorial view.The ornomentation they were reticulate, microreticulate and foveoate.The chemical study also importance in separation of the genus taxa four compounds Flavonoids diagnosed in all the studing species but different in concentrations, by using HPLC, they are Myriscetin, Quercetin, Kaempferol and Coumarin esculetin and this confirms that there are a common evolutionary link between the species in terms of their chemical properties, which suggests belonging species to the genus Euphorbia.Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical districts. the species of Euphorbia were distributed in all Iraqi districts especially in LCA, MSU, MRO, and DWD and some species were farmed in gardens and nurseries either to the beauty of their bractes colors or because their acceleration to the environmental changes such as succulent species, As well as wild species were spread in mountainous areas, oak, nuts forests, in orchards, cultivated fields, flat plains and desert areas in loam soils, clay, gravel, and sand. Through this study some new taxa where founded in Iraq and they are E. cyparissias Kunth ; E. maculata L. ; E. serpens L. ; E. tirucalli L., and this study suggests to recorded them for the first time in Iraq.

دراسة تصنيفية لانواع مختارة من نباتات ذوات الفلقتين في محافظة كربلاء المقدسة, العراق == Taxanomical Study For Selected Dicot. Spp. In The Holy Karbala Province, Iraq

Author name: زينة خليل ابراهيم البهادلي
Supervisor name: علي حسين عيسى الموسوي | خليل ابراهيم الشمري
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: تناول البحث الحالي دراسة بيئية لعدة مواقع من محافظة كربلاء المقدسة بين شهري شباط وايار ولسنتي 2013 و2014 من حيث نوع الترب والمجتمعات الاحيائية لهذه المواقع جمعت خلالها انواع مختلفة من النباتات تابعة لعائلات مختلفة ضمن ذوات الفلقة الواحدة وذوات الفلقتين اذ | The present work includes ecological study for different locations of the holy Karbala province between February and May and for the years2013, 2014, investigating soil kinds and communities in these locations. Different plant species of Monocots and Dicots were collected from variable soil kinds, such as sandy saline soil, loamy saline soil and sandy gravely soil. The species of Haloxylon salicornicum, Zygophyllum coccenium were dominant in the communities of sandy saline soil, Nitraria retusa, Juncus sp. and Halocnemum strobilaceum were dominant in loamy saline soil.Comparative anatomical study has been done for (30) Dicots wild species grown in these environments of Karbala province, as follow : a.Family : Aizooaceae1) Aizoon hispanicum L. b.Family : Chenopodiaceae2) Chenopodium murale L.3) Halocnemum strobilaceum (Pall.)M.Bieb.4) Haloxylon salicornicum (Moq.)Bunge ex. Boiss.5) Salicornia herbacea L.c. Family : Compositae (Asteraceae)6) Carthamus oxycantha M.B. 7) Launaea capitata (Spreng.)Dany 8) Launaea mucronata (Forssk.)Muschl. 9) Rechardia tingitana (L.)Roth. d. Family : Convolvulaceae10) Convolvulus oxypyllus Boiss. e. Family : Cruciferae (Brassicaceae)11) Diplotaxis harra (Forssk.)Bioss. 12) Sterigosella africana (L.)Boch. 13) Sterigosella grandiflora (Bnge)Bochantsev 14) Savignya parviflora (Del.)Webb. 15) Neotorularia torulosa (Desf.) Hedge & J. Leonard 16) Zilla spinosa (Turra)Prantl. f. Family : Cucurbitaceae 17) Citrullus colocynthis (L.)Schrad. g. Family : Geraniaceae18) Erodium laciniatum (Cav.) Willd.h. Family : Malvaceae19) Malva parviflora L. i. Family : Neuradaceae 20) Neurada procumbens L. j. Family : Nitrariaceae21) Nitraria retusa (Forssk.)Aschers.22) Peganum harmala L. k. Family : Papillionaceae23) Trigonella stellata Forssk. m. Family : Polygoniaceae 24) Rumex versicarius L. n. Family : Resedaceae25) Oligomeris linifolia (Vahl)Macbr.q. Family : Solaneaceae 26) Lycium barbarum L.r. Family : Tamaricaceae 27) Tamarix florida Bge.28) Tamarix macrocarpa (Ehrenb.)Bge.u. Family : Zygophyllaceae29) Fagonia glutinosa Del. 30) Zygophyllum coccenium L. The present research focused on many anatomical characters and studies comparatively, such as epidermis types, stomatal complexes, leaf venation, cross sections of root, stem and vertical sections of leaf blades. Different cross sections were shows variability cuticle thickness and epidermis, vascular cylinder tissues, collenchyma and sclerenchyma in different species studies. Variations were noticed among these tissues in the variable species.Thickness of cuticle epidermis of leaves were variable, and unifacial and bifacial mesophylls were noticed for the first time in some species studies. Some cross sections were obtained for scale leaves in Tamarix florida, Tamarix macrocarpa, Halocnemum strobilaceum, Haloxylon salicornicum and Salicornia herbacea.The anatomical characters of these plant parts has not been studied before for these species, at least for Iraqi specimens. The following species Launaea capitata, Oligomeris linifolia and Zilla spinosa were glabrous in all vegetative and reproductive organs. In time that the other species, Haloxylon salicornicum , Salicornia herbacea, Launaea mucronata, Reichardia tingitana, Strigosella grandiflora , Peganum harmala, Lycium barbarum, Tamarix florida and Tamarix macrocarpa were glabrous just in the vegetative parts, but hairy (pubescent) in the reproductive organs. The indumentums was variable in thickness within variable plant organs and parts, even on the lower and upper leaf surfaces. Different kinds of crystals were found and verified in the stems and leaves of different species. Size and density of these crystals were variable in variable locations of stem and leaf tissues.. Geographical distribution was investigated for all the studied species using Iraqi satellite maps by GIS program. Neotorularia torulosa was the most distributed species, and Rumex versicarius was the least distributed one. Schedules, schemes, illustrations and micrographs were presented for the different species studied in this work. Lastly results were discussed depending on available references and reasons.

دراسة تصنيفية لانواع الجنس Echinops L. (Compositae) في العراق == Comparative And Systematical Study of The Genus Echinops L. (Compositae) In Iraq

Author name: سكينة عباس عليوي
Supervisor name: علي حسين عيسى الموسوي
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: تناول البحث الحالي دراسة تصنيفية مقارنة لانواع الجنس Echinops L. في العراق حيث شملت الصفات المظهرية والتشريحية وحبوب اللقاح، وكذلك البيئة التوزيع الجغرافي فضلا عن المعاملة التصنيفية. شملت الدراسة المظهرية دراسة صفات الجذور والسيقان والاوراق والرؤوس الزهري | The present investigation dealt with comparative systematic study of the genus Echinops L. (Compositae) in Iraq.The work involved comparative morphological and anatomical characters, in addition to palynology, ecology, geographical distribution and taxonomic treatment for these species. Morphological characters of roots, leaves, involucre bracts, inflorescence, in addition to, indumentum variations were discussed and the study concluded that stems, leaves, involucres bracts, especially the inner most layer characters were useful aid in distributing species of the genus. Comparative anatomical characters were used, such as characters of leaves, epidermis stomatal complexes, variation patterns, cross sectionings of stems, leaves as well as L.S of growing points. The study revealed that the anatomical characters are important taxonomically. The present investigation observed that pollen grains of all species were tricolporate and echinate. The taxonomic important of grains characters were assessed of the species. Maps of geographical distribution were prepared for all species studied. The study shown that most species were distributed in the North area of Iraq and a few species of the genus were distributed in the East and South of Iraq. In conclusions, (11) species of the genus Echinops were recognized in Iraq. Taxonomic treatment was carried out for all Echinops species and key was designed for them

اليات استحثاث المقاومة في نبات الخيار بواسطة Trichoderma harzianum وBacillus subtilis وGlomus mosseae اتجاه الفطر Rhizoctonia solani المسبب لمرض سقوط البادرات == Resistance Mechanisms In Cucumber Plant By Trichoderma Harzianum, Bacillus Subtilis And Glomus Mosseae Against Rhizoctonia Solani Causing Seedling Disease

Author name: رباب مجيد عبد الذهيبي
Supervisor name: علي هاشم الموسوي | هادي مهدي عبود
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: نفذت هذه الدراسة للتحري عن اليات استحثاث المقاومة في نبات الخيار ضد الفطر الممرض Rhizoctonia solani بوساطة المعاملة بالعوامل الاحيائية وهي harzianum Trichodermaو Bacillus subtilis وGlomus mosseae وتاثير ذلك في نمو نبات الخيار وحاصله النامي تحت ظروف البيت | This study was carried out to investigate the mechanisms of induced resistance in cucumber plant against pathogenic fungus Rhizoctonia solani by bioeagent factors Tricoderma harzianum fungus, Basillus sibtilis bacteria and mycorrhizal fungus Glomus mosseae. The effect in parameters growth and yield of cucumber plant which growing under greenhouse and field conditions. The pathogenic fungus showed high Pathogenesis on cucumber plant. Three isolated of B. subtilis and T. harzianum showed high antagonism ability against R. solani under laboratory condition. For mycorrhizal fungus G. mosseae isolated was tested to study their ability in cucumber growth for chosen the best isolated which can used in anther experiment which results were as follows : - A - Pots experiment 1 - The bioeagent factors was showed their ability to induced several mechanisms of resistance in cucumber plant against pathogenic fungus R. solani, Which included increasing the activity of defense enzymes in existed of pathogenic fungus R. solani, The treatment (T. harzianum + G. mosseae) was recorded highest level in enzyme activity of Phenylalanine ammonia layase enzyme which was (25.3541) unit/gm fresh weight, While T. harzianum treatment showed significantly increased the enzyme activity of Polyphenol oxidase and Peroxidase which was (10.6707, 2.6137) unit/gm fresh weight respectively. This study showed no enzyme activity for chitinase enzyme excepted in some treatment and some periods.This study recorded increased in plant content of total phenol, lignin, salicylic acid hormone, total protein, The (G. mosseae + B. subtilis) treatment overtaken on other treatments in increased plant content of total phenol which was (1.989) mg/gm fresh weight, B. subtilis treatment was significantly increased plant content of lignin which was (18.318) mg/gm fresh weight, The treatment (T.harzianum+ B. subtilis) showed significantly increased plant content of salicylic acid hormone which was (1.8658) mg/gm fresh weight. This study recorded no Jasmonic acid hormone activity in treated plant except in some treatments but with low level, The treatment (G. mosseae + T.harzianum + B. subtilis) was recorded highest content in total protein which was (239.3) ?g/mg. 2 - The rate of seedling death and disease index was significantly reduced in bioeagent treatments, The lowest rate for seedling death in the treatment of (G. mosseae + B. subtilis), (G. mosseae + T.harzianum) and (G. mosseae + T.harzianum + B. subtilis) which were 11 % each for them comparison with control treatmentwith pathogen which was 100%.3 - The rate number of spores and percentage of mycorrhiza infection was significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. The treatment of mycorrhiza infection with pathogen recorded highest number of mycorrhizal spores which was (1410) spore. The mycorrhiza infection reach to 100% in (G. mosseae + T.harzianum) and(G. mosseae + T.harzianum + B. subtilis) treatment with pathogen fungus. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest index of mycorrhiza infection which reach to (80)%, But the index of mycorrhiza infection and mycorrhizal root weight were decreased in the existed of pathogenic fungus, Mycorrhizal dependency it was increased in all treatments with pathogenic fungus and the treatment of (G. mosseae + B. subtilis) with pathogen recorded highest percentage in Mycorrhizal dependency which was (58.873)%. 4 - The plant content of chlorophyll (a, b, total) was significantly increased, The tratment of (G. mosseae + B. subtilis) with pathogen recorded highest level in chlorophyll a it was (5.30)mg/gm, the highest level in chlorophyll b found in(G. mosseae + T.harzianum + B. subtilis) without pathogen it was (6.07)mg/gm, (G. mosseae + T.harzianum + B. subtilis) treatment without pathogen recorded highest level in total chlorophyll it was (10.79)mg/gm. The concentration of phosphor concentration increased in shoot part of (G. mosseae + T.harzianum) without pathogen which was (0.538)%, potassium concentration were decreased in all treatments with pathogen excepted (G. mosseae + T.harzianum) treatment, The highest level of potassium in treatment of (B. subtilis) without pathogen which recorded (4.65)%, For nitrogen concentration was increased in all treatments with pathogen and the highest level in (T.harzianum) which was (5.114)%. Carbon concentration significantly decreased in all treatments with pathogen and (G. mosseae + B. subtilis) without pathogen recorded highest concentration in carbon which was (46.203)%. 5 - The result showed that all growth parameters either in the absence or presence of pathogenic fungus was significantly increased in all bioagent treatments.The percentage of germination reach to (100)% in (B. subtilis) with or without pathogen , the treatment (G. mosseae + B. subtilis) with pathogen recorded highest level in shoot length it was (92.40)cm, And the same treatment recorded highest rate in fresh and dry weight for shoot part which was (7.412, 0.990) gm respectively, (T.harzianum) without pathogen recorded highest rate in leaf area it was (453.083)cm2, The highest rate of flower number was in (T.harzianum) with pathogen which was (15.66)flower/plant. The fresh and dry weight for root, root length, root surface area and the rate of root diameter were significantly increased in comparison with control treatment without pathogen. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest level in fresh and dry weight for root which were (0.359, 0.094)gm respectively. Highest root length in (G. mosseae + B. subtilis) without pathogen it was (48.14)cm, root surface area decreased in the treatments with pathogen and the treatment (G. mosseae + T.harzianum + B. subtilis) without pathogen recorded highest root surface area it was (19.16)cm 2, The root diameter was increased in(G. mosseae) with pathogen was reach to (0.363)mm.B - Field experiment 1 - Under field condition the percentage of germination increased in the treatment without pathogen which were (94.44)% in treatments (G. mosseae) , (B. subtilis) and (G. mosseae + T.harzianum) respectively, In the treatments with pathogen the percentage of germination reduced and The bioeagent factors showed no reduction in percentage of disease infection or disease index with pathogenic fungi R. solani under field condition which reach to (100)%. 2 - The rate number of spores, percentage of mycorrhiza infection, index of mycorrhiza infection, mycorrhizal root weight and Mycorrhizal dependency on cucumber plant significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. (G. mosseae + T.harzianum + B. subtilis) significantly overtaken on other treatments in increased rate of mycorrhizal spors it was (3950)spore, The mycorrhiza infection reach to (100)% in all treatments, For index of mycorrhiza infection the treatments of (G. mosseae + B. subtilis) and (G. mosseae + T.harzianum) recorded highest rate which reach to (100)%, (G. mosseae + T.harzianum + B. subtilis) treatment recorded high increased in mycorrhizal root weight and Mycorrhizal dependency which were (545.67 gm, 67.78%) respectively. 3 - The plant content of total chlorophyll was significantly increased, The highest level in(G. mosseae + T.harzianum + B. subtilis) treatment which was 5.19 mg/gm. There was significantly increased in the concentration of nutrition elements P, K, N, C in cucumber plant. The treatment (T.harzianum+ B. subtilis) recorded highest concentration in phosphor and nitrogen which were (489.4, 3.62) % respectively, The treatment of (G. mosseae + T.harzianum + B. subtilis) recorded highest concentration of potassium which was (2.975)%. The highest concentration of carbon recorded in B. subtilis treatment which was (34.823) %. 4 - All shoot, root and yield parameters of cucumber were significantly increased under field conditions. The treatment (T.harzianum+ B. subtilis) recorded significantly increased in plant height, dry shoot weight, leaf area rate number of flowers, rate number of fruit and fruit weight which were (42.87 cm, 10.85 gm, 631.06 cm2, 19, 17, 1275 gm) respectively. The treatment G. mosseae recorded significantly increased in fresh shoot weight which was (32.76) gm. The treatment with (T.harzianum+ B. subtilis) recorded significantly increased in fresh and dry weight of root, root length, root surface area, rate of root diameter, rate number of fruit and fruit weight which were (11.4 gm, 5.5 gm, 151.71 cm, 44.05 cm2, 0.763 mm, 17, 1275 gm) respectively,

استخدام الزراعة النسيجية والفطر Glomusmossaes في الازالة الحيوية لعنصري الرصاص والكادميوم وتحمل الملوحة لنبات السيسبان Sesbaniarostrata L == Application of Plant Tissue Culture And Glomusmossaes In Bioremediation of Lead, Cadimum And Salinity Using Sesbaniarostrata L. Plant

Author name: التفات فاضل شحاذة الطائي
Supervisor name: علي هاشم الموسوي | كاظم محمد ابراهيم
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت تجارب عدة لغرض دراسة تحمل نبات السيسبان Sesbania rostrata L. للكادميوم والرصاص وكلوريد الصوديوم على مستوى النبات الكامل ومزارعة النسيجية. استحث الكالس واديم على وسط موراشيج وسكوج 1962 والمجهز 0.3 ملغم/لتر من الكاينتين و2 ملغم/لتر من حامض 2, 4 داي مث | Several experiments were carried out to study cadmium, lead and sodium chloride tolerance at the tissue culture or whole plant levels of Sesbania rostrata. Callus was induced and maintained on Murashige and Skooge (1962) medium (MS) supplemented with 0.3 mg/l Kinetin and 2 mg/l 2, 4 - dichlorophenoxy acetic acid (2, 4 - D) using cotyledons as the source for callus induction. Different concentrations of cadmium, lead and sodium chloride directly or gradually were added to the culture medium as contaminants. Selected tolerant cell lines were subjected to regeneration. Callus showed better tolerance to gradual exposure than direct addition. The concentrations of Potassium, lead, Cadmium and Sodium recorded to 17, 24, 27, 35 ppm when 1.0, 2.0 mg/L and 0.4% of cadmium, lead and sodium chloride were added respectively. The study included the effect of the cytokinin (benzyl adenine, BA) and the auxin (Naphthalene acetic acid, NAA) on the regeneration of shoots from callus tolerant to pollutants reached 76, 87 and 85% for callus tolerant to Cadmium, Lead and sodium chloride respectively, when the combination of 0.7 mg/l BA and 0.2 mg /l NAA was supplemented to the medium. The effect of indole butyric acid (IBA) on rooting of shoots. The concentration 0.7 mg /l gave highest percentage of rooting amounted to 66, 70 and 85% in a medium supplemented with cadmium, lead and sodium chloride respectively. Acclimatization of plantlets using river sand soil recorded 85% survival. The study was also included effects on plant height and fresh weights, shoot fresh weight was superior at the concentration 1.0 and 2.0 mg/l of cd and pb recording 8.7 and 7.5 g respectively. Treatment with NaCl led to increase the root fresh weight recording 19.1 g. Maximum cd accumulation reached 12.5 ppm in shoots, 10.5 ppm for lead in roots, 39 ppm for NaCl in roots. The experimental work investigated the effect of mycorrhiza (Glomus mosseae) on cd, pb and NaCl removal by Sesbania plant when grown in either soil contaminated with cd, pb and NaCl or non - contaminated soil (control). Inoculation with mycorrhiza led to an increase in seed germination percentage (96%), plant height (28 cm) in non - contaminated soil, while achieved an increase in shoot and root fresh weight recording 10.0 and 6.8 g respectively. Inoculation with mycorrhiza also led to a significant increase in cd and pb accumulation in roots reached 9.0 and 87.4 ppm respectively. Meanwhile, Mycorrhiza caused a significant increase in Na accumulation in root system (42.5 ppm) which already affected K concentration as a result of competition with Na recording 16.5 ppm in shoots. Mycorrhiza inoculation led to a significant increase in number of root nodes in non - contaminated soil recording 86%. Finally, mycorrhiza succeded in infecting Sesbania rostrata roots reached 90% in non - contaminated soil while it was 85% in contaminated soil

الاكثار الخضري لنبات Spilanthes acmella (L.) Murr. خارج الجسم الحي == In Vitro Vegetative Propagation of Spilanthes Acmella (L.) Murr

Author name: انسام زهير جاسم الحسني
Supervisor name: بشرى محمد جابر علوش
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: وظفت تقانة زراعة الانسجة لاكثار نبات Spilanthes acmella (L.) Murr. كونه نباتا غير مستزرع في العراق ولاهميته الطبية والزراعية بوصفه نبات زينة فضلا عن استعمالاته الاخرى. عقمت البذور بمادة هايبوكلورات الصوديوم(NaOCl) وزرعت على وسط Murashige وSkoog (1962) (MS | The technique of plant tissue culture has been manipulated to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized by sodium hypochlorite (NaOCl) and cultuared on full strength Murashige and Skoog medium(1962) (MS). Nodal segments, apical shoots, and leaflets explants were taken from seedlings and cultured on (MS) medium enriched with different concentrations of Indol acetic acid (IAA), Naphthalene acetic acid (NAA) Benzyl adenine (BA), and 6 - furfurylaminopurine (Kin.). The study include the effect of node orientation vertically and horizontally on induction of shoots formation. The effect of the interaction between IAA and BA, IAA and Kin. on shoot multiplication was investigated. Rooting was also studied after inclusion of IBA and IAA to full and half - strength (MS) medium. At acclimatization stage, different ratios of river sand and peat moss as agricultural media were tested and plantlets survival was recorded. DNA isolation was carried out from fresh and dry plantlet leaves as well as from seeds and Agarose Gel Electrophoresis (AGE) was done. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. The nodes cultured vertically exhibited relatively highest response as compared with apical shoots and leaflets culture. Supplying the culture medium with 1 mg/L. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding IAA at 0.1 and Kin. at 0.5, 1.0 or 1.5 mg/L. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 14.20, 13.60 shoots/ explants with a mean length of 3.40, 2.60, 1.40 cm, respectively. Adding 0.1, 0.5, 1.0 mg/L IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots, respectively, with mean length of 3.25, 3.80, 3.80 cm, respectively. Plantlets were acclimatized successfully achieving 100% survival after four weeks when transferred to agricultural medium consisted of river sand and peat moss at 1 : 1 v/v ratio. This study showed the ability of in vitro micropropagation of Spilanthes acmella (L.) Murr. DNA isolation from drying leaves was butter than fresh leaves with the precense of liquid nitrogen.

دراسة تصنيفية لبعض مراتب الجنس Ranunculus L. (Ranunculaceae في العراق == A Taxonomic Study of Some Taxa of The Genus Ranunculus L. (Ranunculaceae) In Iraq

Author name: علي طالب محمد الطائي
Supervisor name: مازن نواف عبود العاني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: “The current research dealed with fourteen taxa belonging to the genus Ranunculus L., Family Ranunculaceae that grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also were studied and the chemical compounds (Flavonoids), Ecological and geographical distribution and taxonomic treatment have been done. Also botanical key was puted to separate the Taxa”.“General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens in the Iraqi herbaria were studied after ensuring that it was correctly identified”. “Comparative morphological study was undertaken to all plants parts of taxa including the roots, leaves, stipules, flowering parts and fruiting parts. Charts, diagrams, tables were drawn and accompanying with natural images for different morphological characteristics, as well as the field images that are important for the study. This study showed that all the taxa of the genus were herbs that are annual or perennial. There was agreat important for the roots, stems and there Indumentum, the presence orabsence of stipules, shape and size and margin of basal leaves and cauline leaves and leafy bract, the shape of sepals and their numbers and their Indumentum, the numbers and shape of Petals and their colors, the number of Stamens and the colors of Anthers, the shape of ovary and their indumentum, presence or absence of style as it was very useful for the taxonomic identification and separation of the studied taxa. As well as the fruiting parts including the length of fruiting peduncle, the fruiting receptacle, the fruiting head and its shapes and their dimensions, the number of Achene Fruitlets and their sizes, colors and Beak shape. Also the surface configuration of the fruitlets Surface has been studied by (SEM) as it wae either hairy or tuberculate - hairy or Transversely rugose or muricate or reticulate or reticulate - minutly rugose or granular or granular - hairy or granular - hairy tuberculate or spinulose”.“The study of the micro - morphological characters of hairs, leaves epidermal cells and the stomatal complex, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa”.“The study show that the pollen grains have a taxonomic importance because of their difference in shape, dimensions, the wall thickness and their ornamentation. It was either apolar which was spheroidal and pantoporate, or isopolar which has different shapes in the equatorial view it was spheroidal, subprolate and oblate - spheroidal, it was either 3 - colpate, 4 - colpate or 5 - colpat.the colpate where either zonocolpate or syncolpate in the form of parasyncolpate. The ornamentation were verucate, echinateand scabrate”. “The chemical study also has an importance in separation of the genus taxa, six flavonoid compounds were diagnosed by using” HPLC wich are Kaempferol, Vitexin, Orientin, Quercetin, Iso - vitexin and Iso - orientin. According to the presence or absence of those structures cluster analysis where done for the results of the flavonoid compounds and the taxa where divided into groups that reveal the chemical relationships between them.dendogram where performed which also show the chemical relationships between the taxa by using UPGMA method and NTSYS program issue 21. the study shows that the presence and distribution of those compounds vary according to the variation in the plant distribution the study shows that some of the taxa have “the same compounds which enhance its belonging to the Ranunculus genus”. “Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical. Most of the taxa were distributed in N. and N.E of Iraq regions districts especially in MAM, MSU, MRO, and FBF districts. We notice that MSU district has a great number of the plants under the study as we found there are all the taxa accept one”. This study helps to detect distribution areas for some taxa that have been not mentioned in the Iraqi flora or other records which are R.kotschyi and R.trichophyllus var.trichophyllus. Also the study records distribution for the new species R.chius in MAM district which hasn't been mentioned in the Iraqi flora in this district before. Finally all the taxa were classified and the botanical key was done to separate them.

دراسة تصنيفية حياتية مقارنة لمراتب من الجنس Onobrychis Mill. (Fabaceae) في العراق

Author name: زينب جواد عامر
Supervisor name: عذية ناهي سلمان المشهداني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: استــهدف البــحث الــحالي دراســة تصــنــيفية مقارنة لاحدى عشر مرتبة تصنيفية تابعة للجنس Onobrychis Mill. من العائلة الفراشية (البقولية) Papilionaceae النامية بريا في العراق وهي O. acaulis وO. caput - galli وO. carduchorum وO. crista - galli بضربيه O. cri | A comparative systematic study was carried out for eleven taxa belong to the genus Onobrychis Mill., Family Papilionaceae grown widely in Iraq, and they are O. acaulis, O. caput - galli, O. carduchorum, O. crista - galli with two varieties O. crista - galli var. crista - galli and O. crista - galli var. trilophocarpa, O. galegifolia, O. haussknechtii, O. kotschyana, O. megataphros, O. ptolemaica, O. schahuensis. The Study included the gross general Morphological characters and Micromorphological characters, also the Pollen grains studied and Anatomy, Chemistry, Enviroment and Geographical distribution and Taxonomic treatment, also botanical key was putted to separate the taxa. General survey for the Phytogeographical districts where the taxa of genus distributed, in order to detect the distribution of the studied taxa of the genus Onobrychis. Therefore many collections obtained and new locations of taxa distribution were recorded, the specimens studied, and correct the mistake naming specimens were identified and reidentified some miss identified specimens. A comparative morphological study was undertaken to all taxa plants parts include the root, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying, with natural images for different morphology characteristics, as well as the field images. This study indicates that the characters of Leaf, Inflorescence, Flowers and Fruit were collectively contributed in the separation and identification of taxa. The pollen grains have a taxonomic importance because their different shape, dimensions of polar and equilateral view and wall thickness. The anatomical study showed the importance of Taxonomic support to the morphological characters in the diagnosis and isolation different taxa, and this study showed different in the anatomical characters of leaf include the characters of Epidermis, Stomata and the characters of a cross sections in stems. The current study showed that the trichomes were very important taxonomic in isolate different taxa because their variation in density, dimensions and the shape of epidermis cells that the trichomes arise from them and dimensions of the epidermal cells and the nature of their walls. The current study identified nineteen protein - amino acids by using High - Performance Liquid Chromatography (HPLC) and the distribution of these compound was variable among various taxa of the genus and that was useful in isolation different taxa. and the aliphatic amino acids, Glutamic acid, Glycine, Serine, Arginine, Alanine, Tyrosine, Valine, Methionine, Isoleucine and Lysine had a wide presence in the studied taxa, which means that they belong to the same genus. The ecology and geographic distribution of the taxa were studied using area survey and information of herbaria specimens, maps have been drawn for taxa distribution in the Iraqi natural geographical provinces. At last, a taxonomic treatment has been carried out for the taxa of the genus Onobrychis, as well as a detailed key to isolate them.

تحديد بعض المعادن الثقيلة والتلوث البكتيري في الحليب الخام والمستورد المجفف == Determination of Some Heavy Metals And Bacterial Contamination of Raw And Imported Powder Milk

Author name: مروة صباح طالب
Supervisor name: محمد نافع علي العزاوي
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: يتعرض الحليب للعديد من الملوثات الفيزيائية والكيميائية والبيولوجية وذلك بسبب طرق الانتاج واسلوب النقل وعمليات التسويق ومن هذه الملوثات المعادن الثقيلة والرقم الهيدروجيني ودرجة الحرارة والبكتيريا. وقد صممت الدراسة الحالية لفحص عينات للحليب الخام التي جمعت | Milk is subjected to various physical, chemical and biological pollutants duo to producing, transporting and marketing processes such as certain heavy metals, pH, temperature and bacteria. The current study was designed to examine fresh milk samples were collected randomelly at early morning from six different locations at a rate of once each month during study period which commenced in October 2013 and ended in march 2014 and powder milk brands which were examined again for six months as in case of fresh milk where these milk brands were collected from local markets each month. The results of heavy metals showed that : • The investigation of Lead content in raw milk revealed that the highest mean value (1.801±0.311 ppm) was scored in Abo - Ghraib sample and the lowest mean value (0.941±0.104 ppm) was observed in Ghazaliya sample. For the Cadmium content in raw milk that the highest mean value (1.532±0.124 ppm) was found in Abo - Ghraib sample and the lowest mean value (0.063±0.044 ppm) was found in Fal'loga. The Copper content in raw milk also investigated and resulted the highest mean value was (0.931±0.092 ppm) in Azizia milk sample and almost similar value (0.931±0.301 ppm) was found in Madain sample while the lowest mean value was (0.308±0.029 ppm) in Fal'loiga sample. The highest mean value of Potassium in raw milk was recorded in two samples in Azizia (9718.8±246.6 ppm) and (9718.3±482.2 ppm) in Abo - Ghraib but the lowest mean value (4156.3±268.3 ppm) was recorded in Essaouira. Regarding Sodium content in raw milk, the results have found that the highest mean value was (2968.8±312.2 ppm) in Essaouira sample while the lowest mean value was (1432.5±156.8 ppm) in Abo - Ghraib. The Chloride content in raw milk samples, the highest mean value was (3053.0±128.6 ppm) recorded in Fal'loga sample and the lowest mean value was (639.0±198.4 ppm) was found in Azizia sample. Analysis of variance of these data showed significant effects (P?0.001) of both sampling sites and collecting months on milk (pb, Cd, K, Na and Cl) contents while analysis of variance of these data reveals no significant impact of sites on milk Cu content (P> 0.05) where no differences were observed between mean value of different collecting sites but shows highly significant (P? 0.001) effects of collecting months.• While powder milk has shown that the highest mean values of Lead (1.225±0.052 ppm) were found in Al - Mudhish brand and the lowest mean value (0.088±0.002 ppm) was detected in Anchor. The highest mean Cd value was (0.184±0.005 ppm) in Nido brand and the lowest mean value was (0.069±0.003 ppm) in Altunsa sample. While the highest mean value of Copper was (1.656±0.254 ppm) in Dielac powder brand and Anchor milk brand had the lowest copper mean value which was (0.767±0.014 ppm). The concentration of Potassium was (9625.0±462.9 ppm) which is the highest recorded in Dielac milk sample and the lowest mean value (3356.8±225.6 ppm) was found in Al - Marai milk sample. While the highest average of Sodium was (2625.0±342.6 ppm) scord in Dielac brand and the lowest average was (1745.0±338.4 ppm) recorded in Al - Marai sample. The highest mean value of Chloride (193.0±41.0 ppm) was recorded in Nido milk brand and the lowest mean value (122.7±22.2 ppm) was found in Altunsa milk sample. However, analysis of variance shows significant differences (P?0.001) between examined milk brands while no such differences were found between these brands (P>0.05) regarding collecting months.From the above results, it seems that the highest content of (Pb, Cd and Cu) are higher than those of standards of the International Dairy Federation (IDF) while contents of (K, Na and Cl) are within the standard requirement according to National Research Council (NRC).Microbial tests of raw milk samples were positive for bacterial growth, as different bacterial species were observed such as Lactobacilli spp. 63.8%, Streptococcus spp. 61.1%, Staphylococcus aureus 44.4%, Escheritia coli 88.8%, Bacillus spp. 72.2%, Salmonella typhi 72.2%, Staphylococcus epidermidis 30.5%, Pseudomonas spp. 47.2%, Micrococcus spp 27.7%, Clostiridia spp. 66.6%, Enterococci spp. 11.1% and K. pneumonia 75% from bacterial contamination of raw milk samples.However, only (11.1%) of the total collected samples of raw milk were suitable for human consumption and (88.9%) unsuitable, indicating that the source of infection or contamination of milk may be due to the animal health, human handler and the environmental factors, e.g., contaminated vessels, polluted water, flies and dust, etc… Obviously, bacterial contamination test of powder milk samples was carried out and negative results were obtained. However, it seems clearly that the opportunity of finding bacterial contamination in powder milk samples may be very rare since milk powder is produced in well designed and modern techniques. Nevertheless, a single bacterial contamination test has indicated to a positive growth of Staphylococcus aureus colonies.

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L == The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

Author name: مروة قيس ابراهيم العبيدي
Supervisor name: ماهر زكي فيصل الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

دراسة تشريحية مقارنة لبعض الانواع البرية من ذوات الفلقتين النامية في محافظة بغداد == Comparative Anatomy of Some Wild Dicots Spp. Grown In Baghdad Province

Author name: زبيدة عبد اللطيف اسماعيل
Supervisor name: علي حسين الموسوي
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: The present investigation dealt with comparative anatomical characters of 29 selected wild species that belongs to different families growing in different parts of Baghdad Province. These species are : 1. Brassica deflexa Boiss.2. Brassica rapa L.3. Cardaria draba (L.) Desv.4. Sinapis arvensis L.5. Sisymbrium irio L.6. Strigosella africana (L.) Boch.7. Stellaria media (L.)Vill.8. Atriplex nitens Schkuhr.9. Chenopodium album L.10. Chrysanthemum coronarium L.11. Lactuca serriola L.12. Sonchus oleraceus L.13. Medicago polymorpha L.14. Melilotus indicus (L.) ALL.15. Vicia sativa L.16. Erodium cicutarium(L.) L'H17. Geranium rotundifolium L.18. Lamium amplexicaule L.19. Malva parviflora L.20. Plantago lanceolata L.21. Polygonum aviculare L.22. Rumex dentatus L.23. Ranunculus muricatus L.II24. Veronica polita Fries.25. Astrodaucus leptocarpus (Hoghst.) H. Riedl26. Urtica urens L.27. Verbena officinalis L.28. Lippia nodiflora (L.) Rich.29. Tribulus terrestris L.Some of these species have been anatomically investigated for the first time.Anatomical characters might be useful for plant biologist for the identification of important wild plants as an additional character at global level. Comparative anatomical characters were used; such as characteristics of ordinary epidermal cells of stems and leaves and stomatal complexes. The epidermis possesses number of important diagnostic character that offer valuable clues for identification, like size, shape, in addition to indumentum of both stems and leaves. Venation system was investigated and compared. Cross sections of root were useful taxonomically especially the thickness of epidermis, cortex, phloem and xylem. The study observed presence of sclerenchyma tissues in the root cortex of some species. Cross sectioning of stems and petioles were also studied. Epidermis, cortex, pericycle, vascular bundle shapes and numbers, pith characters, presence of crystals and tannin filled cells were useful aid in distinguishing species. Vertical sections of leaf blades, shape and number of vascular bundles, thickness of palisade and spongy layers and other mesophyll characters were important taxonomically.ccording to some of these characters, species were divided into groups. This study concluded the presence of variations in characters and these were presented for the first time comparatively, so that, the anatomical characters were a good support to the exomorphological characters in the studied species. Field photographs of the different studied plants and sections of organs were put in addition to numerous tables in this work. The above results were discussed scientifically regarding mainly the environmental factors.

فعالية مستخلصات بعض الطحالب الكبيرة للفطريات الممرضة للنبات == Activity of Some Macro - Algae Extracts Against Phytopathogenic Fungi

Author name: دنيا يوسف محمد يوسف
Supervisor name: عبد اللطيف محمد جواد
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: تضمنت الدراسة الحالية عزل وتشخيص طحالب كبيرة من بيئتين مائيتين محليتين مختلفتين. تم عزل ثلاث طحالب كبيرة هي Cladophora glomerata (N) وEnteromorpha rafsii من منطقه بحر النجف في محافظة النجف, في حين عزل Cladophora glomerata (R) من احد مبازل منطقة الراشدية ش | The present study includes isolation and identification of Macro - algae from two different environmental water bodies. Three Macro - algae were isolated, Cladophora glomerata (N) and Enteromorpha ralfsii from Baher Al - Najaf region in Holy Najaf city. In addition, C. glomerata (R) was isolated from Al - Rashdiya, north of Baghdad.Phytopathogenic fungi from soil and some infected fruits were also isolated. They were identified as Pythium ultimum and Rhizoctonia solani.Water and ethanol were used to extract each algae to evaluate their antifungal activity against isolated phytopathogenic fungi. Different concentrations of these algal extracts had been prepared which are (10, 25 and 50) mg/ml and the antagonistic activity against the isolated phytopathogenic fungi were evaluated, the hot and cold water extracts did not show any antagonistic activity against fungi. However, all these extracts were ignored in the future work for this study. Furthermore, the hot ethanolic extract was more efficient than these of cold ethanolic extracts. Results have been shown that there were significant differences when macro - algal ethanolic extract were used.C. glomerata (R), which isolated from Al - Rashdiya, was more efficient against tested fungi than the same alga what isolated from Baher Al - Najaf region. However, E. ralfsii extract was more efficient against tested fungi than C. glomerata (N). Percentage of inhibition against P. ultimum when hot ethanolic extract of E. ralfsii, C. glomerata(R) and C. glomerata(N) were (88.8, 83.3 and 63.3) respectively.However, results for cold ethanolic extract of the same algae were (63.3, 52.2 and 32.2) respectively when 50 mg/ml of algal extracts were used.Percentage of inhibition against R. solani when hot ethanolic extract of E. ralfsii, C.glomerata (R) and C.glomerata(N) were (94.4, 100 and 78.8) respectively.However, results for cold ethanolic extract of the same algae were (66.6, 64.4 and 48.8) respectively when 50 mg/ml was used. According to the difference in biological activities against those phytopathogenic fungi caused by the same species of macro - algae or different genera from different environments. Due to difference in biological activity of the same macro - algae species Physiochemical parameter for each environments obtained such as air and water temperature, Turbidity (NTU), Electrical Conductivity(EC)and Salinity%, Water pH, CA+2 concentration, Mg+2 concentration, Total Hardness and Total Alkalinity. Results indicated that soaked Cucumis sativus and Capsicum annum seeds in cold and hot ethanolic macro - algae extracts of (E. ralfsii, C. glomerata (R) and C.glomerata (N) for 24 hours were able to protect seed germination that have been grown in a Petri dish contaminated with P. ultimum and R. solani comparing with control. In addition, results indicated that spraying C. sativus and C. annum seedling with these algal extracts within two weeks old again protects these seedlings from the same phytopathogenic fungi either before or after 24 hr. However, results indicated that adding the algal extracts within two months age to the C. sativus and C. annum plants again protects these plants from the same phytopathogenic fungi comparing with control and fungicide treatment. Moreover, results indicated that the active chemical compounds in E. ralfsii was Tannins, Saponins and Flavonoids. While C. glomerata was contained Tannins, Saponins, Alkaloid and Phenols. Results indicated that both hot crud methanolic extract of C. glomerata (R) and E. ralfsii have a lot of active chemical compounds against micro - organisms by using the GC - Mass Spectrometry technology.
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