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النمط الجيني HLA-DQ والحساسية للاصابة بطفيلي Toxoplasma gondii بين النساء الحوامل والمجهضات في محافظة النجف == HLA-DQ Genotyping and Susceptibility to Infection with Toxoplasma gondii Among Pregnant and Aborted Women in Najaf City

Author name: رنا فاضل عبيد هلال الكلابي

Supervisor name: باقر عبيس سلطان | صباح نعمة الفتلاوي

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

استعمار الجلد من قبل المكورات العنقودية الذهبية المنتجه للذيفانات المعوية لدى المرضى المصابين بالاكزيما الاستشرائيه في مدينة النجف /العراق == Skin Colonization by Staphylococcus aureus Producing Enterotoxins in Patients with Atopic Dermatitis (Eczema) in Al- Najaf City/Iraq

Author name: عماد صادق علي الحلي

Supervisor name: كريم ثامر الكعبي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

معدل انتشار المقاومة البلازميدية لمضادات الكوينولون في العزلات السريرية لبكتيريا Klebsiella pneumoniae في مستشفيات النجف == Prevalence of Plasmid-Mediated Quinolones Resistance Genes in Clinical Isolates of Klebsiella pneumoniae in Najaf Hospitals

Author name: زينب جابر هادي

Supervisor name: علي محسن المحنة

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

تاثير الفالزارتان والرزوفاستاتين على التحوير المناعي بعد الاصابة بنقص التروية الدماغية واعادة الترويه == Effect Of Valsartan And Rosuvastatin On Immune Modulation After Brain Ischemia Reperfusion Injury

Author name: كحلاء كاظم عبد

Supervisor name: هاشم رحيم طارش | نجاح رايش الموسوي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

الكشف عن انزيمات البيتالاكتاميز نوع OXA في بكتريا الزوائف الزنجارية المعزولة من مستشفيات النجف == Detection of OXA-Type ?-Lactamases in Pseudomonas aeruginosa Isolated from Al-Najaf Hospitals

Author name: علاء عبد الحسين رسول

Supervisor name: علي محسن المحنة

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

دراسة مناعية وجزيئية للافات الجلدية في النساء الحوامل المصابات بداء القطط == Immunological and molecular study of cutaneous lesions in pregnant women with Toxoplasmosis

Author name: رنا طالب فاخر النفاخ

Supervisor name: باقر عبيس سلطان | زينب حسن الخفاجي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

دراسة جزيئية ومناعية لترافق وتاثير عصيات السل الرئوية سرطان المثانة == Molecular and Immunological Study for the Association and Effect of Mycobacterium tuberculosis on Urinary Bladder Cancer

Author name: زينا محمد عبد الحسين الشامي

Supervisor name: هاشم رحيم طارش | اسعد عبد الحمزة الجنابي

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

دراسة مناعية وجزيئية بداء المقوسات على بعض مستويات الحركيات الخلوية والهرمونات في النساء في محافظة النجف == Immunological and Molecular study of Toxoplasmosis on some levels of cytokines and hormones in women in Najaf province

Author name: هاشم علي عبد الامير الشريس

Supervisor name: علاء جواد حسن | هاشم رحيم طارش

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

دراسة جزيئية ومناعية لبعض جينات الملوية البوابية == Molecular and Immunological study of some virulent genes of Helicobacter pylori

Author name: اسراء سعيد عباس كاظم

Supervisor name: كريم ثامر مشكور

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

دور الاجسام المضادة الذاتية ، الحركيات الخلوية، اليللات التطابق النسجي في امراضية داء السكري == Role of the Autoantibodies, Cytokines, HLA - DR alleles in the pathogenesis diabetes mellitus

Author name: منظر علي عبد الرضا السعيدي

Supervisor name: حسين سرحان الجنابي | جعفر كاظم الموسوي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

بعض الاختبارات الجزيئية والمصلية للكشف عن مرضى داء المقوسات لدى النساء اللاتي عانين اجهاضا == Some Serological and Molecular tests used to identify Toxoplasmosis among Women with Abortion

Author name: كريم غالي محمد

Supervisor name: سلمان عزيز الجبوري | باقر عبيس سلطان

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine

Language: English

University location: Najaf

First pages:

التشخيص المقارن المصلي والجزئي لداء المقوسات وبعض الاصابات الفيروسية لبعض النساء الحوامل اللوات يعانينن من الاجهاض في محافظة النجف الاشرف == Serological And Molecular Comparative Diagnosis of Toxoplasmosis And Certain Types of Viral Infections (Torch) In Aborted Women In Al - Najaf Province

Author name: ذكرى عبد الله محمود المياحي

Supervisor name: باقر عبيس سلطان | صباح نعمة محمد الفتلاوي

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

Abstract: اجريت هذه الدراسة في محافظه النجف الاشرف / العراق، وتضمنت جمع عينات من دم النساء الحوامل اللواتي يعانين من الاجهاض اول مرة او متكررة وكان عدد الحالات 57 حالة. اعمارهن يتراوح بين 15سنة الى40 سنة واللواتي يراجعن مستشفى الزهراء التعليمي والمختبر المركزي وبع | Toxoplasmosis and viral infections such as Rubella , Cytomegalovirus and Herpes virus (TORCH) are feared risk during pregnancy. The rate of toxoplasmosis and of subclinical infected infants at birth with the risk of late manifestation is still unclear, whereas such data are fairly well - known for Rubella , Cytomegalovirus and Herpes virus. The respective major diagnostic issues in pregnancy, the laboratory diagnosis, and its rational use in combination with clinical information are presented , also the value of passive prophylaxis, therapy, and prenatal diagnosis as well as the possible management for diminishing the infection problems in pregnancy.Aims of study : The study was aimed to evaluate the ELISA result for Toxoplasma, rubella, cytomegalovirus (CMV) and Herpes virus (TORCH) IgM and IgG and compare the results of the ELISA with RT - PCR to arbitrate discordant results.Methodology : The present study was carried out on 57 pregnant women, all of them had with bad obstetric history (BOH) of abortion twice or more. Patients were between 15 - 40 years of age, and they were attending Al - Zahraa Maternity and pediatric Teaching Hospital, Al - Hakeem Hospital and some private clinics, in AL - Najaf province during the period from May 2012 till May 2013. Serological evaluation for TORCH infections was carried out by using IgM and IgG Enzyme Linked Immunosorbent Assay (ELISA ) method and molecular technique ( DNA; RNA - RT - PCR). Each blood sample was divided into two part , one part plane tube for separation of serum that were screened for the presence IgM and IgG antibodies against Toxoplasma gondii ,Cytomegalovirus Rubella virus and Herpes simplex virus by using (ELISA test ).The tests were performed according to manufactures instruction.The other part (blood )EDTA tube was used for DNA 0f detection Toxoplasma , CMV and Herpes simplex virus , while RNA extraction of Rubella virus.The blood and serum sample were stored in refrigerator (2 - 8C?) before the analysis.The extracted DNA and RNA were stored at - 20C? till used within 24 hours. All ELISA Results samples were compared with Real - Time PCR result. Statistical analysis : Statistical program SPSS version (20.0) was used to study the significant difference between tests in each type of antibody (IgM and IgG). (X2 - test) was used after detection of normal distribution to the data and appropriate P<0.001 consider significant. Results : Both IgM / IgG was Seropositive to T. Gondii , Rubella , CMV and HSV - 2 which were 49.12/80.70%, 21.05/49.12% , 45.61/70.18% and 1.21/36.20% respectively. Samples were seropositive to IgM and results were retested by RT - PCR method. Maximum number of IgM /IgG seropositive cases of aborted women was 19(32.75%)/57 that associated with Toxoplasma ,Rubella and CMV infection by RT - PCR ( 10(17.54%), 6( 10.53%) and 3(5.26%) respectively ). The ELISA TORCH IgM and IgG assays provided results comparable to RT - PCR indicated significant differences among Toxoplasma and viral infection. The ELISA TORCH infection IgM and IgG assays showed a lower specificity with for Toxoplasma IgM (p=0.001), poor for CMV IgM and moderate for Rubella virus IgM.Conclusion : The accurate diagnosis of TORCH infection should be done by molecular method.

تعقب الليشمانيا الجلدية بالاختبارات التحليلية الوبائية والطفيلية والجزيئية والكيمياحياتية == Tracking of Cutaneous Leishmaniasis By Epidemiological, Parasitological, Molecular And Biochemical Analysis

Author name: سندس نصیف الحجیمي

Supervisor name: باقر عبیس سلطان | محسن عبد الحسین الظالمي

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

Abstract: The present study was performed to identify the species and strain of Leishmania parasite isolated from different endemic areas. It was carried out on cutaneous leishmaniasis (CL) in five Iraqi provinces as following : Al - Najaf province ( Al - Hakeem and Al - Sadder teaching hospitals), Babylon province ( Marjan hospital ), Al - Qadisya province (AL - Sadder Teaching hospital ), Karbala province (Al - Hussain General Hospital) and Kut province (Al - Zehraa Teaching and Al - Kerama Hospitals). It covered seven hospitals as a field of investigations from October 2010 to December 2012.Cellulose acetate electrophoresis has been performed in Walter Reed Institute of researches in USA. A total of 126 cases comprising 48(38%) females and 78(62%) males were studied. The highest infected age group was 21 - 30 years with a rate of 27.8% and the lowest rate was 11.9% at the age group of 10 and less years.Geographical distribution of CL among hospital patients indicated that rural areas were with highest rate (54%) while in urban areas were with(46%).The number of ulcers per one patient differs for each patient. The highest rate of multiple lesions was 73% in comparison with single lesion (27%).The type of infection showed that disease was in wet type (82.5%) more than in dry type (17.5%). Direct smears were made by lesion aspiration,skin scraping and discharged blood methods , by which 90 (71%) patients gave positive result under light microscope.Modified NNN medium and RPMI - 1640 with fetal bovine serum followed by sub - culture in drosophila Schniders media were performed for each case. Out of 126 cases,83(65%) gave positive growth. Further characterization of the causative parasite species and strain made by molecular and biochemical techniques.Out of 83 postive growth culture,only 52 specimens were studied by nested - PCR, using kinetoplast minicircle fraction amplification to detecte the species of parasite.It was found that 45(86.5%) cases in the generation of a 560 bp DNA and 7(13.4%)patients displayed a fragment of 750 bp, corresponding to L. major and L. tropica, respectively.This test revealed that L. tropica and L. major are the causative agents of infection with dominant shifting to the L. major.Cellulose acetate electrophoresis (CAE) was performed for 20 mass cultivated cultures. Isoenzyme profiles of these isolates were compared with reference strains of Leishmania spp. using cellulose acetate electrophoresis and 4 enzyme systems (glucose phosphateisomerase,leucil phosphate,manose phosphate isomerase and 6phosphogluconate dehydrogenase). The results showed that L.major(LV39) isolate were in 20 mass cultivated culture.Most of the CL patients in Najaf province(2011,2012) were from Haidariya in north district. High prevalence of disease was observed in Autumn and Winter.The highest number of cases was recorded during February and December.

مقارنة بين النوع الثاني من مرض السكري ومرض السكري المناعي الذاتي المتاخر الظهور للبالغين باستخدام مؤشرات الخلايا اللمفاوية وملف الحركيات الخلوية == Comparison Between Type 2 Diabetes Mellitus And Latent Autoimmune Diabetes of Adults Using Lymphocytes Markers And Cytokines Profile

Author name: سوسن محمد جبار الحسناوي

Supervisor name: سلمان عزيز الجبوري

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

Abstract: This study was done to investigate whether Type 2 diabetes and Latent autoimmune diabetes of adult (LADA) patients have similar or different immunological profile.Therefore, measurement of serum levels of adhesion molecules (Serum intracellular cell adhesion molecule(sICAM - 1), Serum vascular cell adhesion molecule (sVCAM - 1) and Serum endothelial Selectin (sESelectin)), chemokines (CCL2, CCL3 and CCL4) and cytokines (IL - 6, IL - 1receptor antagonist and IFN - ?) in these patients was done. In addition to the influence of BMI, gender, age and diabetes duration on these systemic immune mediators were analyzed. Also, the relationships of the in vivo levelsof these mediators with micro - & macro - vascular complications in type 2 diabetes & LADA were studied.Three hundred individual were enrolled in this study and were divided into two subgroups : patients and control groups (280 patients& 20 control). From the 280 patients group who were clinically diagnosed as type 2 D.M., 34 patients were GADA positive and considered as LADA cases. Serum samples were taken from The 34 LADA cases, 36 cases of D.M.2 (selected randomly from the remaining 246 diabetic type 2 patients) & 20 healthy control subjects.The prevalence of LADA among D.M.2 patients was 12.1%. A significant difference in family history, BMI, FBS, HA1C & duration of D.M. was found in D.M.2 cases compared to LADA cases. The mean sVCAM - 1level of control group was significantly lower than D.M.2 and LADA group, P<0.05. The sE - Selectin level was higher in LADA group than control and D.M. type 2 group & significantly different when compared to control group (P<0.05). The sVCAM - 1 level had a significant positive correlation (R=0.25, P=0.017) with BMI in all groups. LADA group had significantly higher values of CCL2 while D.M. type 2 had the least values (P=0.002). The mean IL - 6 of D.M. type 2 group was (11.6±3.1), for LADA group it was (12.4±1.7) and for the controls it was 0.95 ± 0.14. There was a statically significant difference between D.M.2 vs. controls, and LADA vs. controls (P<0.05), regarding the mean IFN - ?, it was significantly higher in LADA group than D.M.2 group (87.5 ± 20.3) vs. (40.3 ± 4.5) respectively, (P=0.032).The mean IL - 1receptor antagonist was significantly higher in D.M. type 2 and LADA cases than controls (P<0.05). A significant positivecorrelation between IL - 6 and sICAM - 1, (R= 0.27, P= 0.013) & a highly significant positive correlation between IL - 6 and IL - 1RA, (R= 0.35, P=0.001) were found. IL - 6 & IL - 1RA serum levels in D.M. type 2 patientswere significantly associated with the complications where p - value?0.05. For conclusions, LADA is clinically underestimated among D.M. type 2 patients and increases with increasing age. Also, Levels of CCL2 and IFN - ? are associated with autoimmunity, Levels of adhesion molecules & chemokines are associated with complications of diabetes.

تقييم مستضد البروستات النوعي في مصل مرضى التهاب المسالك البولية == Evaluation Of Prostate Specific Antigen In Urinary Tract Infection Patients Serum

Author name: علي جبار عبد زيد الكلابي

Supervisor name: وسن سامي حميد

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

مدى حدوث اصابة عنق الرحم بالفيروسات الحليمية البشرية ذات الاختطار العالي والتنميط الجزيئي لتلك الفيروسات == Occurrence And Molecular Detection Of High Risk Cervical Human Papillomaviruses Infection

Author name: منال عبد علي فرحان الحميداوي

Supervisor name: سلمان عزيز الجبوري | ازهار موسى الطريحي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

دراسة مايكروبايولوجية لمرض الداحوس المزمن عند ربات البيوت : عزل، تشخيص والمعالجة ا لفعالة == A Microbiological Study Of Chronic Paronychia In Housewives : Isolation, Identification And Effective Treatment

Author name: كريمة امين حسين الخفاجي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

الدور المناعي للمعلمات السرطانية في تشخيص سرطان القولون والمستقيم == Immunological Role Of Tumor Markers In Diagnosis Of Colorectal Cancer

Author name: ضمياء يوسف محمد سعيد الحارس

Supervisor name: جعفر كاظم نعمة الموسوي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

تقيم طرق تشخيصية مختلفة لبكتريا الكلاميديا تراخوماتس في المجاري البولية التناسلية في الازواج العقيمين في النجف الاشرف == Evaluation Of Different Methods For Diagnosis Of Urogenital Chlamydia Trachomatis In Infertile Couples In Najaf

Author name: انعام عبد الجبار رسول يوسف كشكول

Supervisor name: كريم ثامر مشكور الكعبي | صباح نعوة محمد

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

التوصيف الجزيئي لبكتريا السالمونيلا تايفيميوريوم المعزولة من مصادر مختلفة == Molecular Characterization Of Salmonella Typhimurium Isolated From Different Sources

Author name: علاء عبد الكاظم جواد

Supervisor name: علاء هاني حسن الجراخ

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

انتشار مقاومة المضادات الامينوكلايكوسيديه لعزلات Pseudomonas aeruginosa في مستشفيات مدينة الناصرية == Dissemination Of Aminoglycosides Resistance In Pseudomonas Aeruginosa Isolates In Al - Nasseryia Hospitals

Author name: علي عبد العزيز عبد الواحد

Supervisor name: علي محسن نعمة المحنة

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

التنميط المصلي والوراثي للفايروس الدوار المسبب للاسهال عند الرضع == Serotyping And Genotyping Of Rotavirus Causing Infantile Diarrhea

Author name: وسام صالح عبود

Supervisor name: سلمان عزيز الجبوري | محسن عبد نعمة الروضان

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

التهاب القناة البولية البكترية لدى المرضى المصابين بمرض السكري وغير المصابين به في محافظة النجف الاشرف == Bacterial Urinary Tract Infection In Diabetic And Non - Diabetic Patients In Al - Najaf City

Author name: شيماء عادل عزيز

Supervisor name: كريم ثامر مشكور الكعبي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

دراسة بكتريولوجية المصاحبة للانتان النفاسي في مدينة النجف الاشرف == Bacteriological Study Of Puerperal Sepsis In Al - Najaf City

Author name: محمد عباس فرمان السلامي

Supervisor name: كریم ثامر مشكور الكعبي | ثرى جعفر كاظم

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages:

تقييم الانواع الجينية لمورث فايروس التهاب الكبد الفايروسي النمط ج ودور البعوض في نقل الفيروس == Estimation Of HCV Genome Genotyping And The Role Of Mosquitoes In Its Transmission

Author name: كرار محمد عبد السادة

Supervisor name: غانم عبود جابر المولى | هاشم رحيم طارش

General topic: Medicine

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Najaf

First pages: