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تشخيص بعض النواتج الحيوية لانواع من السيانوبكتريا المعزولة من مياه وترب مدينة تكريت ودراسة فعاليتها على بعض انواع البكتريا المرضية والحيوانات المختبرية == Identification of Some Bioproducts From Some Species of Cyanobacteria Isolated From Water And Soil From Tikrit City And Study Their Biological Effects on Some Pathogenic Bacteria And Laboratory Animals

اسم المؤلف: ايمن عوني سليم جاسم
اسم المشرف: حميد سلمان خميس المهداوي | احسان محمود عبد الله
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - البكتريا
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية عزل وتشخيص (19) نوع من السيانوبكتريا من عينات مياه وتربة طينية ومن الصخور من (5) خمسة مواقع في مدينة تكريت ضمن محافظة صلاح الدين، وقد تم اختيار ثلاثة انواع من هذه السيانوبكتريا وهي : Anabaena oryzae, Nostoc linckia, Anabaena laxa اعتم | The current study included Isolation and Identification of (19) species of cyanobacteria from water, clay soil and rocks samples, collected from (5) sites in Tikrit, salah Al - den province. Three species were chosen each species represent the environment which collected from it. These species were Anabaena oryzae, Nostoc linckia, Anabaena laxa. The effects of some environment factors on growth and biological products quantity has been studied in the three species above. The effects of the temperature showed that 26°C was the best for growth and biological products quantity than 16°C and 36°C. While the pH effects showed that pH 7.6 was the best for growth and biological products quantity than pH 6.6, 8.6. Three light intensities were used 1250, 2500, 5000 lux were used. The results showed that the light intensity of 2500 lux was the best for daily growth and quantity of extracted biological products. While the study of the effects of addition of macronutrient and micronutrient to Asm - 1 media which used for culturing of the three species of cyanobacteria by replacement of NaCl by NaNO3 in the medium, showed that there is an increase in daily growth and quantity of extracts of biological extracts, also the addition of FeSO4.7H2O and NaNO3 together showed further increase of daily growth and biological extracts quantity. The biological products has been identificated by HPLC - MS, IR and NMR. The results showed the presence of the neurotoxin Anatoxin - a and the hepatic toxin Microcystin in its various forms MCYST - LAba, MCYST - YM(O), [D - Asp3, Dha7]MCYST - LR and MCYST - YA in the biological products of Anabaena oryzae. The results also showed the presence of the hepatic toxin MCYST - YM(O) and [D - Asp3, Dha7]MCYST - LR in the extracts of the two spices of cyanobacteria Nostoc linckia and Anabaena laxa.The results also showed that the Muller Hinton Agar media is better than the Blood Agar media in testing the activity of these extracts in inhibition of the pathogenic bacteria, the method of holes is better than saturated discs, and the solvent DMSO (Di - Methyl Sulpho Oxide) is the better in testing of biological activities, while the filtrate of cyanobacteria did not shown any activity for inhibition of the pathogenic bacteria which include : Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus Vulgaris, Staphylococcus aureus. The results showed the high capability of inhibition in concentrations of 1000, 2000, 3000 µg/ml of the extracts of the cyanobacteria Anabaena oryzae, Nostoc linckia and Anabaena laxa on the above pathogenic bacteria and some times more efficient than antibiotics used for comparison, and the concentration 3000 µg/ml was the most effective.The effects of biological extracts of the three species in concentrations of (1000, 2000, 3000) µg/kg of body weight on tested albino mice, using injection and feeding methods, was studied. The results showed poisoning nervous symptom for the first few hours, while the results showed hepatic poisoning symptom on the dissecting animals. The anatomic symptoms included changes the colour of the liver to darkening and appearance of necrosis, in addition to becoming lobbed and swollen some times with bleeding.While the poisoning effects on dissecting tissues of livers of the animals showed a simple swollen and some times assured in all of the liver accompanying with increase in growth and cell division, as well as increases in lymphatic cells inside the tissue and in general in liver tissue. The lymphocyte seemed bigger than for normal cell with multi - nuclei in white blood and increase in cell size and destruction of liver tissue. Nostoc linckia was the greater urging for cell division consequently probably more induce of cancer in the liver of the animals, while the biological product of Anabaena laxa showed more poisonous for liver cell than the biological products of Anabaena oryzae, the least effective in cytotoxic and urging cancer cell in liver of the animals.

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات == Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

اسم المؤلف: شيماء عبد محمد علي
اسم المشرف: وعد محمود رؤوف | خالد عمر محمد علي
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children in Sharqat City

اسم المؤلف: علي حمد طلال موسى الجبوري
اسم المشرف: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study for Al - Karkh Drinking Water Plane At Al - Tarmia

اسم المؤلف: احمد توفيق احمد المشهداني
اسم المشرف: رياض عباس عبد الجبار
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

تقييم فعالية مستخلصات مستنبت القمح Triticum aestivum في بعض الانواع البكتيرية المسببة لاخماج مختلفة == Evaluation The Activity of Germinated Wheat Extractions Triticum Aestivum on Some Bacterial Species That Cause Different Infections

اسم المؤلف: اسماء عيسى محمود
اسم المشرف: رشيد حميد حسن
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: The study had included isolation and diagnosis of three hundred samples for both sexes their ages were between (1 - 60) years of the inpatients and outpatients in Tikrit Teaching Hospital and General Samarraa Hospital during the period from 1/9 / 2011 to 1/3/2012. The infections were distributed among the burns infections, the wounds, urinary tract infections (UTI) and female genital tract infections (High vagina). This study clarified that (192) sample from the total samples showed bacterial growth in percentage 64%. The results of this study indicated to the proportion 58% with (UTI) by (87) bacterial isolation were produced from (150) isolation. The percentage of burns infections were 81.81% from (55) sample which distributed among the first, second and third class burns. The burns of second and third class gave bacterial growth in a proportion 100% while the first class burns gave 64.28%. Concerning the wounds infections, bacterial growth was 62.9% from (62) sample. The infections proportion of (H.V) was 63.63% from (33) sample. The results of the current study showed that the infections in females were higher that in males with respect to (UTI), while there was not considered difference between the males and females about the burns and wounds infections. Differently, the (H.V) infections were just for the females in the ages between (18 - 45). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 25.52% from the total isolates followed by Staphylococcus aureus 18.23% Pseudomonas aeruginosa 15.63%, both Klebsiella pneumoniae and Proteus miribilis 10.42% Staphylococcus epidermidis 4.69% Citrobacter freundii 4.17%, Staphylococcus saprophyticus 3.64% both Serratia marcescens and Enterococcus faecalis 2.6%, Streptococcus pyogenes 1.56% and Staphylococcus intermedius had the least percentage 0.5% by one from (192) isolate. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, beta - lactams and tetracyclines antibiotics. Among these antibiotics imipenem and ciprofloxacin were more effective, while the other antibiotics were different either active or not active at all.The wheat germinating was used in this study with the ages (36 - 48) hour and its activity was tested by using some bacterial kinds. After completing several quantitative and qualitative tests on this germinating, the carbohydrates proportion was between 67 - 70%, the protein proportion by folin method was between 10 - 8%, the lipids proportion was 1.5%.Some mineral elements in germinated wheat were estimated which include Calcium, Iron, Magnesium, Potassium, Copper, Manganese, Zinc, Selenium, and Sodium element, active materials were also estimated in the wheat germinating extracts which had inhibitory effect on the microbiology. It was clear by the tests that the germinating contains a lot of Phenolic compounds, Terpeniods, glycosides and steroids. These materials were extracted by using some extracts included the cold water extracts and hot water extracts, alcoholic extracts (ethanol and methanol) and phenolic extract. All the extracts prepared in concentrations (25, 50, 100, 200) mg/ ml. This study showed that the phenolic extract had high effect on the bacterial kinds in comparison with the methanolic and ethanolic extracts at (200) mg/ ml concentration while the cold water extract had less effect on these bacterial isolates in comparison with the above extracts, finally the hot water extract had no inhibitory effect against any isolated bacterial type with all the concentrations. The inhibitory action of these extracts was compared with some antibiotics as a control sample. These antibiotics represents different groups of antibiotics and which contained Imipenem, Ciprofloxacin, Gentamicin, Ceftazidime in addition to Augmentin.In this study the minimum inhibitory concentration (MIC) of the fenolic, alcoholic and watery extracts was determined and the concentration was different according to the extract type, the bacterial type and the isolation site.

دراسة تاثير مستخلصات نباتي القرنفل والخروع على نمو بعض الفطريات المنتجة لانزيم البروتييز والمسببة لالتهاب الاذن الخارجية == Study Effect of Syzigium Aromaticum And Ricinus Communis Extracts on Growth of Some Fungi That Produce Protease And Cause Otitis Externa

اسم المؤلف: بتول عمران ذيب
اسم المشرف: خليل ابراهيم بندر النعيمي | ذكرى احمد حمادة
الموضوع العام: علوم الحياة
السنة: 2009
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الفطريات
الصفحات الاولى:
المستخلص: ضمنت الدراسة عزل وتشخيص الفطريات من الاذن الخارجية في مستشفى تكريت التعليمي - وحدة الاذن والانف والحنجرة والعيادات الخاصة في مدينة تكريت, خلال الاشهر تموز, اب وايلول 2008. جمعت 100 مسحة اذنية من المرضى المشخصة اصابتهم بالتهاب الاذن الخارجية الفطري من قبل | The study was included isolation and identification of fungi from the external ear in Tikrit teaching hospital, the unit of Ear, Nose, and throat (E.N.T.) and Privete clinics in the city of Tikrit during the months July, august, and September2008. 100 ear swabs were collected from the patiants whose diagnosed by Otologists with Otomycosis, and 50 ear swaps were collected from healthy persons in the same city and period time, These samples were examined by dierect microscopic examination treated with KOH 10% with hot flame to ensure the fungal infections, and Culturing on Sabouraud, s Dextrose Agar was done for isolation and identification of types of fungi which responseple for infection, The result of direct microscopic examination and culture showed that 76(76%) were positive for fungi, 64 were positive by direct microscopic examination and culture, and 12 posative in culture only. The most common fungi isolated from patient were Aspergillus Spp.36). (%22 followed by Candida Spp. (%67. 10), Trichophyton Spp(%6.5) , and Penicillium spp. (%3.94%), Aspergills niger appeared as the predominant fungi (%84. 36) followed by Aspergillus flavus (%36. 22), Candida albicans (42. 18%), Aspergills terrous (7.89%) , Candida kruzi, Penicillium spp (3.94%), Trichophyton mentagrophyte, Trichophyton verrucosum (63. 2%) and Trichophyton scheonlieni (%1.31), and no fungi were isolate from the healthy person. Higher percentage of fungi infection were recorded in female 46(60.52%) than male 30(39.47%). and the higher percentage for fungal infection was recorded in the age group (31 - 40) with percentage (23.68%). fungal ear infection was recorded with highest percentage in right ear 47 (61.84%), and for the left ear 29 (38.15%). and high rate of infection was recorded in august (73.69%), Higher percentage of infection was recorded in patient house wives (52.36%), the itching was the most commen symptom , The most habits used to clean the ear by dearty body (51.31%), Ahigher percentage (76.4%) recorded in non educated persons. The results of active ingredients test for Syzigium aromaticum and Ricinus communis revealed that clove dry flower buds contain Flavonoides, Glycosides, Tannins, Volatile oils, Terpenes, Resins, and Phenols, while castor leaves contain Flavonoids, Tannins, Volatile oils, Terpenes, Resins, Saponins and Phenols, pH of clove bud 4.3 and pH of castor leaves 5.8. Aqueous and alcoholic extracts were Prepeared, which belongs to Syzigium aromaticum and Ricinus communis under the study, commercial oil for clove and seed castor were used in the study, the effects of inhibition of Aqueous and Alcoholic extracts and commercial oil had been studied on the growth and protease production on the three fungi which were showed the high ratio appeared among the isolation sample under the study with 5 concentration 10, 20, 30, 40, 50 (mg /ml), and the affected rate of these extracts and oils under the study on fungi were varied. The relation between Aqueous extract of clove bud and fungi growing was increasing relation, While Alcoholic Extract and commercial oil showed preference in the inhibition of the isolated fungi grown while its effect on Candida albicans was increase with increase the concentration, The effect of aqueous and Alcoholic Extract of castor leaves on Moulds showed reverse effect. on the other hand the commercial oil effect was slight effect, and both extracts did not showed effect on the Candida albicans as well as the Commercial oil.When adding Clove Extracts and Commercial oil and in all concentrations dose not gave any production of the Protease enzyme in the solid media, whereas the extracts of castor leaves and commercial oil were showed effects on the production of Protease enzyme, and Aspergills niger was more effected and these extracts did not showed an effect on the Candida albicans.Aqueous and Alcoholic extract and commercial oil to the Clove revealed higher influence rate than the effect of the Castor leaves extract , and in a different rate

تشخيص المسببات البكتيرية والفايروسية لخمج الاغشية السحائية في مدينة الموصل == Detection of Bacterial And Viral Causes of Meningitis in Mosul

اسم المؤلف: قاسم مصطفى خالد
اسم المشرف: علي صالح حسين الجبوري | اميرة محمود محمد الراوي
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمنت الدراسة اجراء عدد من الاختبارات العيانية والكيميائية على عينات سائل النخاع الشوكي (CSF) cerebrospinal fluid البالغة 152 عينة والتي جمعت من الاطفال الراقدين في مستشفى الخنساء التعليمي ومستشفى ابن الاثير التعليمي في مدينة الموصل لحديثي الولادة لغاية 1 | This study includes performing of macroscopical and chemical and Microbial tests on 152 samples of cerebrospinal fluid (CSF) collected from hospitalized children in Al - khansaa and Ibn - Al - Atheer teaching hospitals in Mosul city with ages ranged from newbornn to 12 years old. Suspected to be infected with meningitis during the interval from August 2009 till April 2010.The results showed that 46.1% of samples were normal with respect to colour and appearance as well as variations in the levels of the three variables including glucose, protein and total cell count with differential cells in the studied samples.The study also included isolation and identification of gram positive and negative bacteria, morphological, cultural and biochemical tests and API were done depending on identification of the isolated bacteria.Many types of isolated gram positive bacteria belonged to the two genera staphylococcus and streptococcus; the results revealed that S.aureus was the more predominant within gram positive causes. Well as many types of gram negative bacteria were isolated represented with Klebsiella pneumoniae, Haemophilus influenzae, Acinetobacter spp. E.coli and Neisseria meningitidis with different percentages with dominance of K.pneumonia and H.influenzae.The study also included screening of some viral causes of meningitis from (35) CSF samples which gave negative culture results by using qualitative Enzyme immunoassays technique using Minividas apparatus.The results showed positive results with 14.2% for two types of virus including Herpes simplex virus (HSV) and Cytomegalo virus (CMV).The study indicated that males were more compared with Females with 55.9% and 44.1% to males and females respectively and highest infection rate occurred in 1 day - 2 years old.Statistical analysis showed the presence of significant correlation between sex, age and the three varieties glucose, protein and total cell count the results showed there is no significant differences between the studied factors.The study also includes sensitivity for some antibiotics, the results showed that gram positive bacteria were the more sensitive to Vancomycin and Cefatriaxone as well as Clindamycin while gram negative bacteria showed sensitivity and resistance percentages of bacteria in general were varied to other antibiotics under study.

دراسة تاثير مستخلصات القرفة (Cinnamomum zeylanicum) والسماق (Rhus coriaria) في التثبيط المايكروبي وبعض المعايير الحيوية في ذكور الجرذان == Study The Effects of Cinnamon (Cinnamomum Zeylanicum) And Sumac (Rhus Coriaria) Extracts on Microbial Inhibition And Some Biological Parameters in Rats Male

اسم المؤلف: فراس عدنان حسين الزيدي
اسم المشرف: كركز محمد ثلج الجبوري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: صممت الدراسة في مختبرات قسم علوم الحياة / كلية التربية وقسم علوم الاغذية / كلية الزراعة في جامعة تكريت, اذ تضمنت الدراسة التعرف على المواد الفعالة في المستخلصات المائية والكحولية في كل من قلف القرفة وبذور السماق واختبار فعاليتها التثبيطية عند تركيز 10, 25 | This study was conducted in Biology department laboratory / Education college and Food science department in Agriculture college in Tikrit University. the study was conducted to investigated to the aquaies and alcoholic activities extracts from each Cinnamon bark and sumac seeds and assay of inhibition activity concentration at 10, 25, 50 and 100 mg/wells against gram positive and negative bacteria and phagocytosis Immunity level after injection by E.coli type, further more to study the effect it in some physiological parameters in male rats after oral administration for 8 days by 50 and 100 mg/kg from rats body weight from each aquais and alcoholic extracts. The results was illustrated the aquaies and alcoholic extracts from each Cinnamon bark and sumac seeds were contains the active groups from compound such as Tannins, Resins, Phenols, Flavonoids, Alkaloids, Glycosides, Coumarins. while the effects was found the aquaies extracts a high significantly effects (p < 0.05) from alcoholic by inhibition ability when assay. The Minimum inhibition concentration (MIC) from each ones which mass 50 and 125 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen cinnamon bark aquaies extract. it was 75 and 100 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen from alcohol extracting its inhibiting efficiency throw the measure of the diameter of the inhibiting area that has shown that the gratest effect was E.coli bacteria which its diameter of inhibiting area 17 mm and when the concentration is 100 mg/wells from the alcoholic and aquaies extracting and the less effect is S.pyogen and S.aeruginosa in case of the extraction from cinnamon bark it was 17 mm the diameter of inhibiting area of E.coli bacteria. when the concentration 100 mg/wells from aquaies extracting from sumac. whether with alcohol extraction, the bacterial type were similar in their sensitivity in diameter of inhibiting from 18 to 20 mm but the oral administration of the extract on the level of phagocytosis process through the injection of rats in affixed number from E.coli bacteria. The result was illustrated the type of concentration from each cinnamon bark and sumac seeds such as aquaies and alcoholic was caused an increase in the phagocytosis process through the significantly decrease in the number of bacteria with increase of the concentration from the groups that oral administration from extraction that there number wear (zero) on the concentration 100 mg/kg from rats body weight when the time is 120 minute which was equal with effincy of the ciprofloxacin antibiotic on the concentration 5 mg/kg from rats body weight. The result also showed the effect of oral administration from the extraction of cinnamon bark and sumac seeds such as aquaies and alcoholic, that significantly caused the decreased in the concentration of the hemoglobin and Total count red blood cells and the packed cell volume on the concentration 100 mg/kg from the weight but the measures have not significantly changed the parameters MCV, MCH, MCHC with increase of concentration from extraction compared with the group of controlling. The white blood cells also decreased in the significantly aquaeis and alcoholic extracting from the cinnamon bark it was 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the aquaeis extracting, and 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the alcoholic extracting, That numbers have not affected during the oral administration from the extractions of sumac only on the concentration 100 mg/kg from the body weight of the alcohol extracting which was 5.6 × 103/mm3 the increase was in the ratio of the number of the neutrophils in the whole extraction alcoholic and aquaies for the both of plants. While the effect of oral administration from the extraction in the lipids profiles was clear in the decrease significantly in the concentration of the glucose and albumin and Urea and uric acid and creatinine and triglyceride and, cholesterol, and the low density lipoproteins (LDL) and very low density lipoproteins (VLDL), significant increasing in globulin and high density lipoproteins (HDL) in aquaies and alcoholic extraction of sumac seeds while the value of Total protein and HDL is not significantly differtited in aquaies and alcoholic extraction moreover oral administration of the both of plants. The enzyme activity value was included significantly decreased of the Alkaline phosphtase activity value with the increased orally administration from aquaies and alcoholic extracts from both of plants, while increased significantly Aspartames amenotransferase (AST) activity value in all cinnamon extracts while was significantly decreased when administrate from sumac seeds extract, But don’t significantly differ Alanine amenotransferase (ALT) activity value in all concentration from aquaies and alcoholic extracts from both of plants.

علاقة مستوى الزنك والنحاس ببعض المعايير المناعية في الاصابة بداء المقوسات في النساء في محافظة كركوك مع محاولة علاجية في الفئران المختبرية == Relation of Zinc And Copper Levels With Some Immunological Markers in Women With Toxoplamosis in Kirkuk Governorate With Trial To Treatment in Mice

اسم المؤلف: عبير عباس علي
اسم المشرف: حسين ساهر اسود العبيدي | شهاب احمد محمد
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: تم دراسة 100امراة مصابة بداء المقوسات Toxoplasmosis الذي يسببه الطفيلي المقوسة الكوندية Toxoplasma gondii للفترة من اذار 2007 ولغاية نيسان 2008 راجعن العيادة الاستشارية التابعة لمستشفى كركوك العام في مدينة كركوك. اظهرت نتائج الدراسة الحالية ان 56 % من الن | A total of 100 patients suffering from Toxoplasmosis which is infected by the parasite Toxoplasma gondii have been dealt with as subject of the sample during the period from March 2007 to April 2008 at the Consultant Clinic of Kirkuk General Hospital. The results of the present study showed that 56% of the subjects were pregnant and 44%were not. The highest percentage of the infected subjects was of 26 - 30 years old as 30.37% among the pregnant women and 36.35% among the non - pregnant women of the age mentioned above. The highest percentage 37.5% of abortion of the first period of pregnancy was in pregnant women, and 45.53% in non - pregnant women. The results of the study also showed more cases of infected women among those who bred animals in their living places as 69%. It is also found that the highest percentage of the infected women 64% were of those who use the running water for drinking. Moreover, it is noticed that there is an increase in the percentage of the infected women among the uneducated women 38%. The study showed that the percentage of the infection is proportional to residence 63%, the infection increase among women living in rural areas. The percentage of T - lymphocytes in peripheral blood of patients were also measured through measuring the percentage of the CD4+ and CD8+cells by using fluorescent microscope.The results showed a significant decrease (p<0.05) in this type of cells.The rate of percentage of CD4+ of the patient's were 31.704±0.116 while the rate of CD4+ in the peripheral blood of the control group was 62.260±0.237.The rate of percentages of CD8+ 19.382±0.080, whereas it was 31.680±0.307 of the control group.The rates of the percentages of CD4+ to CD8+ were also measured, the results showed a significant decrease (p<0.05) in the rate of the percentage in the patient's group 1.642 while it was 1.968 in the control group. The statistic analysis showed significant differences between these tow groups (p<0.05). The intensity of Zinc and Copper has been measured in the serum of the infected women via use of Atomic absorption instrument, a significant decrease (p <0.05) appeared in the rate of Zinc intensity in the serum on the infected women as compared with the control group.The rate of the intensity in patient's group was 4.65% ± 2.628µmol /l while in the control group it was 15.353± 1.640 µmol /l.A significant increase (p<0.05)was also noticed in the intensity of Copper in the patient's group (28.589±4.335 µmol /l)whereas it was 12.854±1.501 µmol /l in the control group. The index of the specific antibodies IgM and IgG related to Toxoplasma gondii was measured by ELISA method, a significant increase (p<0.05) was observed in the value of IgM and IgG in the patient's group as compared with the control group. The rate of index for IgM in the patient's group was 1.354±0.05 while it was 0.163±0.05 in the control group. The rate of value of IgG in the patient's group was 1.163±0.074 whereas it was 0.358±0.033 in the control group. In the present study, Toxoplasma gondii was isolated and diagnosed from human as first attempt, and then the infection was created in laboratory mice as an essential step for growing the parasite. The second step was studying the effect of some selected drugs on the experimental treatment for disease of toxoplasmosis created in the mice. The drugs are : Pyrimethamin, Clarithromycin, Spiramycin, Ivermectin.The results of the study showed the effects of these drugs on the tissue cyst formed in the brain and lungs. The number of tissue cysts in the brain of the mouse treated with pyrimethamin was 14.8 ±1.067 after 28 days. Significant differences appeared when this result compared with the control group in which it was 27.4±1.029. The number of tissue cysts formed in lung, tissue in the mouse treated with the same drug after the same period of the time was 6.2±0.583.This showed a significant differences as compared with the control group in which it was 10.2±0.583. No significant differences appeared between both groups as for the treatment by Clarithromycin antibiotic as far as the number of the tissue cysts formed in the brain after 28 days is concerned. The number was 23.8 ±0.86 as compared with the control group 38.6±1.029.The same compared is true of the lungs, tissue. The number of the tissue cysts was 23.8±0.86 as compared with the control group 28.4±1.805. Concerning the antibiotic Spiramycin, the results showed the effectiveness of it in decreasing the tissue cysts formed in the brain.The significant differences were (p<0.05), the rate of the cysts was 9.6±0.6 as compared with the control group in which it was 22.8±1.067.The same effectiveness appeared on the lungs, tissue as the rate of tissue cysts after 28 days of treatment was 4.6±0.509 as compared with the control group in which was 17±1.14 and the significant differences were (p=0.000).The Ivermectin drug showed a great effectiveness in minimizing the number of the tissue cysts formed in the brain and the lungs. The numbers of the tissue cysts formed after 28 days were 3.2±0.374 and 1±0.316 respectively; significant differences are observed when these numbers are compared with the numbers of the control group which were 22±1.843 and 19.4±1.435. Thus, the use of Ivermectin drug is considered as a pioneer and fruitful study since this drug has not been used for the treatment of Toxoplasmosis.

دراسة بعض مظاهر امراضية الخمج بالمتحولة النسيجية بين الاشخاص في مدينة سامراء == A Stud Y of Some Aspects of Entamoeba Histolytica Pathogenicity Among Peoples in Samarra

اسم المؤلف: صلاح عبد حسن السامرائي
اسم المشرف: ابراهيم شعبان داوود | حسين ساهر اسود
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: شملت هذه الدراسة فحص (540) عينة براز من المصابين بالاسهال والمراجعين لمستشفى سامراء العام وذلك خلال الفترة الممتدة بين 1/10/2007الى 1/6/2008 فحصت العينات بطريقة المسحة المباشرة المزدوجة للكشف عن الاطوار الخضرية والمتكيسة لطفيلي اميبا الزحار. واظهرت النتائ | The current study dealt with examination of (540) stool samples for patients with diarrhea who visited the General Hospital of Samarra during 1/10/2007 - 1/6/2008. The samples were examined by double wet direct smear method to identify the trophozoite and cyst stages of Entamoeba histolytica. The results showed that (112) were infected with amoebic dysentery. The total rate was (20.74 %). (262) are males with a rate of infection (19.84 %), whereas, the number of females was (278) with an infection rate (21.58 %). The study showed an increase rate of infection among the rural population, it was (25.71 %), whereas the urban population rate was (16.6 %). The study covered all ages beginning from less than a year to 75 years old. A highest rate of infection was recorded by (10 - 19) years old, it was (29.4%). The study also, found out that a highest rate of infection was during school months, particularly during November (27.5 %). Examinations of complete blood picture which included Hemoglobin concentration (Hb), packed cells volume (PCV), white blood cells count (WBCc), and erythrocyte sedimentation rate (ESR) were done on all patients infected with amoebic dysentery. The results were compared with those of healthy ones (Control Group). The results showed significant differences between the two groups. According to the significant one (P < 0.05), it recorded a decrease (16.9%) in the (PCV) and (25.1%) in the (Hb) and an increase (241.8%) in the (ESR) and (51.5%) in the (WBCc) for those ones infected with amoebic dysentery if compared with the control group. Clinical biochemistry examinations were performed, on blood sugar (Bs), electrolytes such as Sodium (Na+), Potassium (K+), Calcium (Ca++), Magnesium (Mg++), liver functions tests : total serum bilirubine(TSB), tranceaminase enzyme(GOT, GPT), alkaline phosphetase (AlP), total protein (TP) and albumin (Alb). The results showed that there are significant differences between persons infected with amoebic dysentery and the healthy ones according to the significant level (P < 0.05) in these examinations. The study showed a decrease(21.6%) in the (Bs) mean concentration, (16.8%) in the(K+) mean concentration, (5.8%) in the(Ca++) mean concentration, (4.5%)in the (Mg++) mean concentration, (10.5 %) in the (Na+) mean concentration , (17.2%) in the (TP) mean concentration and(12.2%) in the (Alb) mean concentration and increase (83.7%) in the (Alp) mean concentration, (52.1%) in the(TSB) mean concentration, (81.1%)in the (GPT) mean concentration, (59.9%)in the (GOT) mean concentration and(22.8%) in the (globulin) mean concentration in the persons infected with amoebic dysentery compared with the mean concentrations in healthy ones control group.

تحديد تجمعات Giardia lamblia ومعقد Entamoeba histolytica / Entamoeba dispar بالتشخيص الجزيئي (PCR) للمصابين في تكريت == Determination of Giardia Lamblia Assemblages And Entamoeba Histolytica / Entamoeba Dispar Complex By Molecular Diagnosis (Pcr) in Patient in Tikrit

اسم المؤلف: انتصار غانم عبد الوهاب الصميدعي
اسم المشرف: شهاب احمد محمد الجبوري | عقيل حسين العاصي
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للمدة من كانون الثاني 2011 ولغاية كانون الاول 2011 للمرضى الراقدين والمراجعين لمستشفى تكريت التعليمي وبعض المختبرات الخاصة في مركز المدينة وضواحيها والذين يعانون اعراض مرضية مختلفه ولفئات عمرية مختلفة ترواحت بين اقل من سنة الى 61 سنة، ح | This study was conducted for the period started from January 2011 until December 2011 on inpatients and outpatients of Tikrit educational hospital and some private laboratories at Tikrit city and surrounded suburbs. Symptoms of different diseases among various age groups varies between less than one year to 61 years. The tests used the (ELISA & PCR) and the analysis of the PCR - RFLP to discriminate the infection by assemblage of Giardia lamblia and differentiate between pathogenic amoeba Entamoeba histolytica and nonpathogenic Entamoeba dispar compared with microscopical examination. The result of present study showed that (75) person who represent (37.5%) were infected by Giardia lamblia and (75) person who represent (37.5%) were infected by E.histolytica/E.dispare complex, out of (200) stool samples which have been tested by microscopical examination. Epidemiological study showed no significant difference among the sex for the two species parasites whereas a high percentage for infection recorded in group at age (1? - 10) year for Giardia lamblia and E.histolytica / E.dispar complex reach to (60%, 36%) respectively. Regarding the place of living Giardia lamblia in rural area showed infection rate of (19.5 %), while infection rate by E.histolytica/E.dispar complex reaches (22.5%) in urban area. Statistical analysis shows a high difference of significance according the source of water supply. Tap water showed high incidence for initiating infection which reaches to (93.3%) by G.lamblia and (90%) for E.histolytica/E.dispar complex. For the infection rates and their relationship to the educational level, the results show that the highest rates of infection recorded in non - educated (52%) and (73.3%) of Giardia lamblia and E.histolytica / E.dispar complex, respectively. Depending on the sample texture, the result show that oily diarrhea is more correlate with infection reaches to (33.3%) of Giardia lamblia and the watery diarrhea form 40% by E.histolytica / E.dispar complex, while simultaneous occurrence of both symptom (colic and loss of appetite) are the most symptom synchronized with infection records (44%) for Giardia lamblia and (36%) for E.histolytica / E.dispar complex. The percentage of infection by Giardia Lamblia in this study depends on the test results of the ELISA was (20%), while the percentage of infection E.histolytica/E.dispar complex was (6.7%). Testing has shown sensitivity compared to microscopic examination reaches to (22.6%) and (8%) for both parasite respectively, while the test showed a high specificity which reaches the rate (93.3%) and (100%) for both parasites respectively. Result of PCR showed a percentage of infection by E.dispar about (32%) (64 sample) from the total samples, whereas it does register any cases infection by E.histolytica. By counting the results of the PCR for the three genes (ssu rRNA + TPIA + GDH) they show a total infection by Giardia lamblia which was (10.5%) (21 samples). This study recorded percentage of infection Giardia lamblia depending on the result of the three Genes separately amounted to (4%), (5%), (3%) for (ssu rRNA, TPIA, GDH) respectively. According to the result of the analysis PCR - RFLP for GDH gene by using the enzyme NIaIV, it did not record any infection by Giardia lamblia (assemblage A), whereas (6 samples) recorded (100) by Giardia lamblia (assemblage B), by using the enzyme Rsal to determine the secondary assemblage shows that the total (6 samples) infected there are 5 samples carrying infection by two type of secondary assemblage returning to the assemblage B (assemblage BIII +BIV) and only one sample infected with (assemblage BIV). To determine the assemblage and secondary assemblage for the parasite result compared with amplification of TPIA gene with lysis by enzyme for the resulting amplification for GDH gene, resulting a ratio of (14.3%) for a sample infection by both assemblage (A+B) and secondary assemblage (BIII+BIV) together, and a ratio of (57.14%) infection by assemblage A only and (21.4%) infection by secondary assemblage (BIII+BIV) together and (7.14%) infection by BIV only.

دراسة التاثير المثبط لبعض انواع بكتريا حامض اللاكتيك ضد بعض انواع الفطريات الممرضة والمنتجة للسموم == Study The Inhibition Effect of Some Species of Lactic Acid Bacteria Against Some Pathogenic And Toxins Producing Fungi Types

اسم المؤلف: لؤي برهان مصطفى محمد
اسم المشرف: كركز محمد ثلج الجبوري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: اجريت الدراسة في مختبرات قسم علوم الحياة في كلية التربية بهدف عزل وتشخيص بعض انواع بكتريا حامض اللاكتيك ومحاولة اختبار قابلية خلاياها او النواتج الايضية منها وكذلك دراسة تاثير المستويات المختلفة من درجة الحرارة والاس الهيدروجيني في القابليـة التثبيطية لب | This work aimed to investigate the effects of some lactic acid bacteria (LAB) species (cells or their metabolic products) at different temperatures and pH levels on some soil pathogenic or toxin produced fungi species. Six LAB isolates and the identification was depended on the morphological and cultural characterized and biochemical testes, the isolates were determined as Lactobacillus delubricii subsp. delubricii, Lactobacillus delubricii subsp. bulgaricus, Lactobcillus casei, Lactobacillus acidophilus, Leuconostoc mesentroides and Streptococcus thermophilus, and four fungal species Microsporum canis, Trichophyton mentagrophytes, Aspergillus fumigatus and Rhizopus stolinfer were identified. In this study the highest inhibition effect against the above mentioned fungi was shown by L. acidophilus as a significant (P< 0.05) efficacy where the diameter of inhibition zone in case of M. canis, T. mentagrophytes, A. fumigtus and R. stolonifer was 26, 32, 30 and 28 mm respectively. Less inhibition activity was exhibited by Leu. mesentroides and Str. thermophilus while the lowest inhibition was shown by L. delubricii. Results showed also that the temperature optimal for the growth of bacterial species (except L. casei and Str. thermophilus) tested and their inhibition activity was 30 °C. However, temperature of 35 °C was the optimal for the highest inhibitory effect of both L. casei and Str. thermophilus. At 25 °C of studied bacterial species showed decreased inhibitory effect against the tested fungi. Concerning the pH, the present study showed that the highest inhibitory of all tested bacteria species (except L. acidophilus) was at pH 6.5, L. acidophilus showed such activity at pH 5.5. This study revealed that the inhibitory effect of metabolites produced mixture of the tested bacterial species was directly proportional with its concentrate. Where this mixture was used at media concentration of 10, 20 and 40 mg/ml cultural. However, metabolic products from L. acidophilus, Leu. mesentroides and Str. thermophilus was the most effective in inhibition activity compared with the rest of the bacteria species used particularly at 40 mg/ml cultural media

دراسة بكتريولوجية لبيئة بعض كهوف شمال العراق والتحري عن الدور الجرثومي فيها == A Bacteriological Study on Some North Iraqi Caves Environment And Detection on The Bacterial Role in It

اسم المؤلف: امنة غانم عمر العاني
اسم المشرف: جهاد ذياب محل الجنابي | اميرة محمود محمد الراوي
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمنت الدراسة اجراء عدد من الفحوصات البيئية والبكتريولوجية على عينات الصخور ومياه النضح البالغ عددها 100 عينة والماخوذة من بعض كهوف المنطقة الشمالية في العراق التي شملت بهيري, شرانش, خنس, بجيل وانشكي للفترة ما بين تشرين الثاني - 2010 لغاية نيسان - 2011.ت | This study was included some of ecological and bacteriological tests on rocks and dripping water samples which were included 100 samples taken from some of north region caves in Iraq including Beherii, Sharanish, Khanas, Bejeel and Inishky from the period between November - 2010 to April - 2011.The results of ecological tests which represented with cave environment temperature were ranged from (13 - 24)?C and dripping water temperature were ranged from (11 - 20)?C while the results of pH values for the same sample shows simple variation between (7 - 8) for dripping water and (6 - 9) for the rocks samples. The results of geological screening and mineralization by using X - Ray Fluorescence and X - Ray - Diffraction showed that Beherii cave was mainly composed of manganese with 102 ppm comparing with other caves. While the concentration of iron and sulfur oxides were very low for all caves. The rocks of Beherii and Khanas caves were mainly composed of Dolomite (CaMg (CO3)2), Sharanish cave was composed of Calcite (CaCO3) and Dolomite with the closed ratio, while the rocks of Bejeel and Inishky were composed mainly of Calcite. The isolated bacteria in this study were identified according to cultural and morphological characteristics and biochemical tests, while the bacillus species were identified by using Microgen Bacillus Id System. Twenty two bacterial isolates were identified from total samples. There were nine (9)identified isolates from Beherii cave with 40.9% including Arthrobacter spp., Lysobacter spp., Caulobacter spp., Bacillus freudenreichii and Bacillus subtilis in with 4.5% for each isolate, while there was 18.1% of Methanogenic bacteria with its four genuses including Methanobacterium spp., Methanobrevibacter spp. Methanospirillium spp. and Methanosata spp. In Sharanish cave there was one isolate of sulfur oxidizing bacteria with 4.5 % from the total isolates. Three isolates were identified from Khanas cave included Pseudomonas spp., Xanthobacter spp., Clostridium spp. with 4.5% for each isolate. In addition, it was obtained from a cave Bejeel on six isolates included Bacillus lichiniformis, Bacillus lentus and 2 isolates of Bacillus cereus group, Streptomyces spp. and Gallionella spp. with 4.5% for each isolate and Merismopedia spp., Myxococcus Xanthus, Clostridium spp. were Isolated from Inishky cave with 4.5%for each isolate. The relation between some of bacterial species were studied by detection of the lytic ability of Lysobacter spp. and Myxococcus xanthus for Arthrobacter spp.The microbial role in caves were studied by detection of the ability of some isolated bacteria in Calcium Carbonate (calcite) formation and estimatation of calcite production, Xanthobacter spp. was the highest productive bacteria which gives0.2073 gram during 14 days comparing with other bacterial species.In addition, the effect of temperature and pH on calcite formation by Bacillus freudenreichii were studied, the results showed variation on calcite shape in 20?C and 30?C while the highest value of calcite production in pH.8 was 0.1336 gram comparing with pH.9 with 0.0190 calcite gram. The study also detected the ability of some isolated bacteria to oxidize of iron, manganese and sulfur, where Streptomyces spp. Gallionella spp. and one isolate of bacillus cereus group have shown the ability of oxidize iron on leptothrix medium while Bacillus freudenreichii, Bacillus lichinformis, Bacillus lentus, one isolate of Bacillus cereus group and Streptomyces spp. have shown the ability to the oxidize of manganese on Krumbein and Altmann Agar Medium. In addition, sulfur oxidizing bacteria has shown ability to oxidize sulfur on M.S. Agar and produce sulfuric acid as a product of the oxidation process.

عزل وتشخيص بكتريا Streptomyces المنتجة للمضادات الميكروبية من ترب بعض اقضية محافظة صلاح الدين == Isolation And Identification of Antimicrobial Producing “Streptomyces” From Soils of Some Districts in Salahaddin Province

اسم المؤلف: هيام عزيز عباس البدري
اسم المشرف: عبد الكريم فتاح عمر
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - البكتريا
الصفحات الاولى:
المستخلص: تضمنت الدراسة عزل وتشخيص انواع تابعة للجنس Streptomyces، اذ جمعت 50 عينة من ترب اقضية تابعة لمحافظة صلاح الدين. شخصت?11 نوع من بكتريا Streptomyces باستخدام الاختبارات الشكلية والمزرعية والكيموحيوية حيث ظهر كل من الانواع (Streptomyces antibioticus, Strep | The Study involved ; isolation and characterization of species related to Streptomyces. Fifty samples of soil has been taken from different districts of Salah aldden province. The isolates were identified by the morphological, biochemical tests and classified to nine species, The following species were found : (Streptomyces antibioticus, Streptomyces coelicolor , Streptomyces distalicus and Streptomyces fluvissimus ) in Tikrit district, ( Streptomyces cyaneus ) in Ballad and beiji districs, ( Streptomyces exofolicus) in Ballad and Aldor districts (Streptomyces lavendulea ) in Samarra and Aldor districts , (Streptomyces purpureus ) in Samarra and Beiji districts and (Streptomyces violaceus ) in Samarra and Tikrit districts. The sensitivity of these bacterial isolates were tested toward the following : (Azithromycin, Cefotaxime, Cephatexin, Doxycycline, Erythromycin , Gentamicin, Neomycin, Penicillin, Tobramycin and Vancomycin).All species showed high resistance to cefotaxime. But all species were sensitive to neomycin and doxycycline. The isolated species of Streptomyces were tested for their ability of antibiotic production. It was found that (Klebsiella) was sensitive to all the species except species Streptomyces coelicolor. Streptomyces coelicolor showed an effect against Proteus. While Staphylococcus aureus was sensitive to the following species (Streptomyces antibioticus, Streptomyces cyaneus and Streptomyces purpureus). The Scanning Electron Microscopy was used to study the surface of spores. The species of (Streptomyces antibioticus) was found to has warty surface while spores of ( Streptomyces coelicolor) has smooth surface.

عزل وتشخيص بعض انواع بكتريا السالمونيلا من بعض الاغذية المحلية في مدينة تكريت == Isolation And Identification of Some Species of Salmonella From Some Local Foods in Tikrit City

اسم المؤلف: شيماء جابر حميد البياتي
اسم المشرف: عبد الكريم فتاح عمر
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تم التحري عن بكتريا السالمونيلا في 520 عينة من الاغذية المحلية في مدينة تكريت, وجدت هذه البكتريا في 26 عينة (5%), كانت اعلى نسبة لتواجدها في عينات اللحوم المثرومة (2.5%) والتي تمثل 16.25% من العينات الكلية للحوم المثرومة المدروسة.عزلت السالمونيلا من عي | The presence of Salmonella spp. was investigated in 520 local food samples in Tikrit city, Salmonella was found in 26 samples (5%), with the highest presence in minced meat (2.5%) which was represented 16.25% of all minced meat samples. Salmonella was isolated from chicken's meat at a percentage of 3.75%, and same percentages for meat slices samples, egg cortex and chicken's blood represented 5% for each one, and isolated too from vegetables and cheese at a percentage of 2.5% for each one. No Salmonella has been detected from sweet, yoghurt and egg content. Six Salmonella species were isolated, S. typhimurium was to be the most frequent and represented 42.30% of positive samples, then S. enteritidis and S. anatum in 34.61% and 11.53% respectively, but S. agona, S. typhi and S. arizona existed in a percentage about 3.84% for each one. Also, all the isolated species showed their own serological formula. The selective medium Tetrathionate broth (TTB) was found to be superior for isolation of Salmonella than Selenite cystine broth (SCB), the former positivity was 96.2% and 88.5% respectively. For test of better selective medium for isolation, TTB was found to be more suitable for isolation of S. anatum, while SCB was better for isolation of S. arizona.All the six isolated Salmonella species showed high sensitivity to Ciprofloxacin (5?g) and moderately sensitive to Chloramphenicol (30?g), while they showed high resistance to three antibiotics Amoxicillin (30?g), Nalidixic acid (30?g) and Pipracillin (100?g). They showed resistance with different percentages to the other tested antibiotics : Ampicillin (10?g), Gentamicin (10?g), Streptomycin (10?g), Tetracycline (30?g) and Trimethoprim (25?g).

مقارنة لتشخيص الخمج بالمقوسات الكوندية بين النساء المجهضات والولودات طبيعيا مع دراسة للتغيرات النسجية المتسببة عن الخمج == Comparative Diagnosis of Toxoplasma Gondii Infection Among Aborted & Normally Delivered Women With Study of Histological Changes As A Result of Infection

اسم المؤلف: فراس محمد بشير عبد الكريم الخشاب
اسم المشرف: ابراهيم شعبان داوود | صباح سعيد محمود
الموضوع العام: علوم الحياة
السنة: 2009
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة التي امتدت من بداية شهر ايار (2007) لغاية نهاية شهر اب (2008) للتحري عن نسبة الخمج بالطفيلي المسبب لداء المقوسات بين النساء المجهضات والولودات طبيعا في بعض مناطق مدينة الموصل والمراجعات لمستشفى الخنساء التعليمي للولادة والاطفال.شملت عي | This study was conducted during the period from May 2007 to August 2008 to investigate the rate of infection with toxoplasmosis among aborted and normally delivered women in Mosul City territories who attending AL - Khansa'a educating hospital.Out of 150 women under study, 50 were aborted while 100 were normally delivered. The employed tests were included latex agglutination test LATEX and Enzyme Linked Immunosorbent Assay (ELISA) with its two types (ELISA - IgM), (ELISA - IgG) which used as a high specific and sensitive test to differentiate between types of infection acute, chronic and congenital. Toxoplasmosis was diagnosed in 28(56%) out of 50 abortive placenta and in 57(57%) out of 100 non abortive placenta (normally delivered women), these samples of placenta were microscopically examined after homogenizing and staining by Gimsa and Leishman's stain The parasite development stages were also noticed including developing from the tachyzoites into the oocysts stage and the histopathological effects resulting from infection in the placenta in both aborted and normally delivered women which were represented by necrosis, vaculation and cells degeneration , inflammatory cells infiltration and the focal inflammatory reaction in the villi in addition to hemorrhage and accumulation of eosinophilic substances, the results also showed that 41 (82%) of aborted placenta and 18 (18%) of non abortive placenta (normal delivered women) were infected with Toxoplasmosis by using the laboratory Balb/c mice inoculation method while, some cases of inflammation were diagnosed in the brain, of the experimental infected mice, which were represented by the inflammatory cells infiltration and the prevailing of gliosis, tissue necrosis vaculation and cells degeneration.This study revealed that laboratory mice inoculation in the peritoneal cavity is a useful and efficient method in the detection of the parasite in infected placenta as compared with the microscopic examination of direct smears and examination of tissue sections from placenta.The present study showed an overall percentage of infection among aborted women was (92%) while (58%) showed an acute infection and (34%) chronic infection, in another hand the infection rate among normally delivered women was (70%), and it has been found the infection rate among acute and chronic cases were (41%), (20%) respectively, Its to be noted this is the first time in Mosul City for such a study conducted in this area concerning the congenital Toxoplasmosis in newborns with an infection rate (9%).In this study, sera were tested by Latex (LAT), and Enzyme Linked Immunosorbent Assay (ELISA). It was shown that IgG - ELISA is the most sensitive to diagnose the infection as compared to other tests.These study also concerned with the isolation and diagnosis of Toxoplasma gondii parasite from blood by inoculating the blood samples in laboratory Balb/C mice the rate of infection was (16%) in laboratory mice inoculated with blood collected from aborted women while the infection rate in laboratory mice inoculated by blood samples (mother and her neonatal cord blood) collected from normally delivered women appear in (12%) & (16%) respectively. The rate of occurrence of the specific antibodies against Toxoplasma gondii was identified, The highest rate was found for the type (IgG+ - IgM+), in both aborted and normally delivered women with percentage reach (40%) & (26%). The titer (100 IU/ml) of the specific IgM & IgG antibodies was found in high rate reached (75%), (43.5%) in mother blood, also found in a high percentage in the neonatal cord blood estimated as (50%), (64.4%) for the specific IgM & IgG antibodies respectively, in another hand the titer (100 IU/ml) was also found to be the more predominate titer in blood samples of aborted women with percentage reached to (50%) & (60.5%) for the specific IgM & IgG antibodies respectively.

دراسة بيئية ومايكروبايولوجية في شركة مصافي الشمال

اسم المؤلف: سعاد حمود محمد حماد البشار
اسم المشرف: رشيد حميد حسن السامرائي
الموضوع العام: علوم الحياة
السنة: 2001
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:

التهاب الكبد الفيروسي نوع بي وسي : دراسة وبائية مصلية وتحديد النمط الجيني في مدينة سامراء == Hepatitis B And C Virus Seroepidemiology And Genotypes in Samarra City

اسم المؤلف: كيلان عبد الرزاق شاكر ياسين الدوش
اسم المشرف: عبد الغني محمد السامرائي | عبد الكريم فتاح عمر
الموضوع العام: علوم الحياة
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الفيروسات
الصفحات الاولى:

الاصابات الطفيلية في بعض اسماك عائلة الشبوطيات وعائلة البياح في نهر دجلة المار في مدينة تكريت == Parasitic infections of some cyprinid and mugilid fishes from Tigris river passing through Tikrit city

اسم المؤلف: ميساء ابراهيم علي الجبوري
اسم المشرف: فاطمة شهاب الناصري
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:

دراسة وبائية المقوسات الكوندية Toxoplasma gondii بين الازواج في مدينة تكريت ومحاولة تجريبية عن امكانية انتقال الخمج جنسيا بين الفئران من نوع Mus musculus == Epidemiological study of Toxoplasma gondii between couples in Tikrit city , and experimental trial about possibility of sexual transmission of infection in mice type Mus musculus

اسم المؤلف: مقصود عادل محمود الدوري
اسم المشرف: توفيق ابراهيم الالوسي | عبد الله حسين الجبوري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:

دراسة تشخيصية لبكتريا Helicobacter pylori المسببة لمرض التقرحات المعدية في محافظة صلاح الدين == Diagnostic Study for Helicobacter pylori Bacteria That Cause Gastric Ulcers in Salah Aldeen Governorate

اسم المؤلف: مصعب علي جواد المهداوي
اسم المشرف: امين سليمان بدوي
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - البكتريا
الصفحات الاولى:
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