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التوصيف المظهري والجزيئي والوظيفي لبعض الاحياء المجهرية المعزولة من اللحوم الحمراء والبيضاء في محافظة النجف == Morphological, Molecular and Functional Characterization of some Microorganisms Isolated from Red and White Meats in Najaf Province

Author name: بـسـعاد عبد زيد عبود الفتلاوي

Supervisor name: سامي عبد الرضا علي

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

دراسة بكتريولوجية و وراثية لعزلات Klebsiella pneumoniae المقاومة لمضادات Quinolones

Author name: حسين علي حسين

Supervisor name: ماجدة مالك متعب

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls - Department Of Biology

Language: English

University location: Najaf

First pages:

التحديد الجزيئي لمورثات البيتلاكتاميز في العزلات السريرية لبكتريا ال Morganella morganii == Molecular Detection of Beta - Lactamase Genes in Clinical Isolates of Morganella morganii

Author name: صديق غني جودة المحنا

Supervisor name: عباس شاكر المحنة

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

دراسة بعض عوامل الضراوة لبكتريا E.cloacae وتاثيرها في تحفيز الاستجابة المناعية للحيوانات المختبرية == Study of some E.cloacae virulence factors and their role in stimulation of immune response in Lab animals

Author name: بشائر احمد العميدي

Supervisor name: مهدي حسين العمار

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

دراسة بكتيريولوجية وجزيئية لبكتريا Enterobacter spp. المعزولة من عينات مرضية مختلفة == Bacteriological and molecular study of Enterobacter spp Isolated from different clinical specimens

Author name: انتظار نعيم عبد

Supervisor name: مهدي حسين محيل العمار

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

الكشف المظهري والجزيئي لبكتريا الزائفة الزنجارية المقاومة لمضاد الكاربابنيم في مستشفيات النجف الاشرف == Phenotypic and Molecular Detecting of Carbapenem Resistant Pseudomonas aeruginosa in Najaf Hosp

Author name: جمال محمد رضا علي الشرع

Supervisor name: علي محسن نعمة المحنة

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - College Of Science

Language: Arabic

University location: Najaf

First pages:

الكشف عن دور الزوائف الزنجارية في اصابة القناة التنفسية عند الاطفال باستخدام تقنية تفاعل البلمرة المتسلسل المتعدد في محافظة النجف == Detection the Role of Pseudomonas aeruginosa in Respiratory Tract Infection in Children by Using Multiplex - PCR in Najaf Province

Author name: علي عبد الحسين الفتلاوي

Supervisor name: حوراء عبد الامير الدهان

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science

Language: English

University location: Najaf

First pages:

تاثير فوسفات الصوديوم في بكتريا الزوائف الزنجارية المعزولة من اصابات القناة البولية خارج الجسم الحي وداخله == Effect of sodium phosphate in Pseudomonas aeruginosa isolated from Urinary tract infections In vitro and In vivo

Author name: مروة عبد حسين المحمدي

Supervisor name: عبد المجيد عبد الله السعدي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science

Language: Arabic

University location: Najaf

First pages:

توصيف مظهري ووراثي لبكتيريا Moraxella catarrhalis المعزولة من اصابات القناة التنفسية == Phenotypic and Genotypic Characteristics of Moraxella catarrhalis Isolated From Respiratory Tract Infections

Author name: زهراء عبد الحسين المؤمن

Supervisor name: عبد المجيد عبد الله السعدي | حوراء عبد الامير علي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

دراسة جزيئية لعوامل الضراوة لبكتريا Klebsiella spp. المعزولة من خمجات الجروح والحروق == Molecular Study For Virulence Factors Of Klebsiella spp. Isolated From Wound And Burn Infections

Author name: رؤى شلتاغ نايل الرديني

Supervisor name: عباس شاكر المحنة

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

دراسة امكانية دور الابشتاين - بار فايروس في التهاب اللوزتين المزمن == Study the possible role of Epstein - Barr virus in chronic tonsillitis

Author name: موسى نعمة مزهر الجعيفري

Supervisor name: محمد عبود محسن | اسعد عبد الحمزه الجنابي

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

توصيف عزلات الفطر Aspergillus spp. الحاملة لجين aflR الملوثة لبعض الاغذية في اسواق النجف الاشرف وامكانية مقاومتها احيائيا == Characterization of Aspergillus spp. Isolates that are carrying the aflR Gene That pollute some Foods in Al - Najaf Al - Ashraf Markets and the capability of resisting them biologically

Author name: ابتسام بشير كاظم الساعدي

Supervisor name: سامي عبد الرضا علي الجميلي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

توصيف الانزيمات الحالة للبروتينات والدهون والمنتجة من البكتريا العصوية Bacillus subtilis == Characterization of Proteases and Lipases Produced by Bacillus subtilis

Author name: ليلى صالح عبد الحسن المعموري

Supervisor name: عبد المجيد عبد الله السعدي | زهرة محسن علي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

دراسة تاثير المضاد الحيوي ومحددات عوامل الضراوة في بكتريا المكورات المعوية المعزولة من المرضى المصابين بالبيلا البيكتيرية في النجف الاشرف == Study of Antibiotic Susceptibility and Virulence Determinants among Enterococcus faecalis Isolated from Patients with Significant Bacteriuria in Najaf

Author name: كوثر عدنان حسن الياسري

Supervisor name: علي محسن المحنة

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

دراسة الفعالية التضادية لبكتريا Lactobacillus acidophilus في نمو بعض انواع البكتريا المعوية وبعض الجوانب المناعية داخل الجسم الحي وخارجه == A Study of the Antibacterial Activity of Lactobacillus acidophilus on Some Enteric Bacterial Growth and Some Immunological Aspects in Vivo and Vito

Author name: فاديه مهدي مسلم العميدي

Supervisor name: مهدي حسين محيل العمار

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls

Language: Arabic

University location: Najaf

First pages:

عزل وتشخيص الفطريات المسببة لمرض تبقع الاوراق البني على الرز واستخدام المكافحه المتكامله على المرض في محافظة النجف == Isolation and Identification of Fungi causing of Brown Leaf spot Disease on Rice and use the complete

Author name: موسى نعمه مزهر

Supervisor name: سامي عبد الرضا الجميلي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science

Language: Arabic

University location: Najaf

First pages:

انتاج سماد حيوي من لقاح سلالة البكتريا Azospirillum irakense

Author name: سعاد وحيد كاظم

Supervisor name: سامي عبد الرضا الجميلي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science

Language: English

University location: Najaf

First pages:

دراســــة الفعالية التضادية لجرثومة Bifidobacterium spp تجاه جرثومة Staphylococcus aureus المقاومة للمثيسلين == Studying The Antagonistic Activity of Bacteria Bifidobacterium Spp Against Bacteria Methicillin Resistance Staphylococcus Aureus

Author name: انغام نجاح هادي الخفاجي

Supervisor name: سهام جاسم الكعبي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

Abstract: تضمنت هذه الدراسة التحري عن الفعالية التضادية لجرثومة Bifidobacterium spp تجاه عزلات سريرية لجرثومة Staphylococcus aureus المقاومة للمثيسلين، ودراسة تاثير تلك المواد على عوامل الضراوة المنتجة من قبل S.aureus المقاومة للمثيسلين مثل الغشاء الحيوي Biofilm وا | This study included the investigation of Bifidobacterium spp antagonistic activity against Staphylococcus aureus isolates, and the effect of these antimicrobial agent on virulence factors produce by MRSA (Methicillin Resistance Staphylococcus aureus) such as Biofilm and Slime layer. One handered different samples were collected to isolate Bifidobacterium spp and 50 clinical isolates of Staphylococcus aureus from clinical specimens in AL - Najaf AL - Asharaf governorate during the period of (10/10/2013 - 20/1/2014) by the cultural characteristic colonies, microscopic for cells, biochemical tests and polymerase chain reaction PCR technique to detect 16SrDNA, the results showed that 13 isolates belong to Bifidobacterium spp and there are divergent gene content between these isolates and they have been bearing lm26/ lm3 gene. Carbohydrate fermentation test was used to distinguish the species of Bifidobacterium, the results revealed that the B.bifidum is more predominant than the other species; B.thermoacidophilum, B.adolescentis, B.breve, B.longum. Antibiotics susceptibility test to Staphylococcus aureus using antibiotic disc diffusion assay showed that Methicillin Resistant Staphylococcus aureus formed 70% and 30% of isolates were sensitive to the same antibiotics, also the ability of MRSA isolates to produce Biofilm and Slime layer was investigated by tube method and Congo red agar, then 10 isolates of Staphylococcus aureus were selected as efficient isolates according to the resistance of Methicillin and the high ability to produce biofilm and Slime layer. The antagonistic activity of Bifidobacterium spp was extracted and tested against these MRSA isolates, the results showed that the most isolates of Bifidobacterium spp were effective against Methicillin Resistant Staphylococcus aureus (MRSA), as well as the inhibition of growth and formation of Slime layer by using the wells and disc diffusion agar assay. The inhibition zone was between (10 - 30)mm, and the ability of bacteriocin production of Bifidobacterium spp against MRSA have been conducted by well diffusion broth method, B.bifidum3 was greater than other species in producing bacteriocin with asignificant increase (P<0.05). Furthermore the impact of bacteriocin production to inhibit the development and formation of biofilm was investigated, the results showed that the bacteriocin has ahigh activity against MRSA. The bacteriocidal and bacteriostatic effect of bacteriocin was conducted by using spectrophotometer, the results showed that these effects were bacteriostatic in the beginning of the growth MRSA while it was lethal during other time and 40 minutes is the period needed for killing MRSA bacteria.

عزل وتشخيص المبيضات من المرضى المصابين بداء السلاق الفموي في محافظة النجف الاشرف مع دراسة جزيئية لبعض عوامل الضراوة == Isolation And Identification of Candida Spp. From Patients With Oral Thrush In Al - Najaf Province And Molecular Study of Some Virulence Factors

Author name: ايهاب يحيى جابرالعلياوي

Supervisor name: فاطمة عبد الحسين التميمي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

Abstract: تشمل هذه الدراسة بعض المعايير السريرية لمرضى نقص المناعة (مرض السكري والسرطان وزرع الكلى)، والمصابين بداء المبيضات الفموي والتي تضمنت العمر والجنس. الهدف من هذه الدراسة هو عزل وتشخيص انواع المبيضات. بطرق مختلفة بما في ذلك التشخيص المباشر، والتحضين في الم | The present study was conducted to isolation and identification of Candida spp. Isolated from immunocompromised patients with (Diabetes, Cancer & Kidney transplant) by different identification methods including direct examination, laboratory culture, biochemical tests and molecular method using polymerase chain reaction (PCR) technique and determine the virulence factors phenotypic to Candida spp. and genetic polymorphism in Candida albicans in secreted aspartyl proteinase (SAP2). During the period from October 2013 to February 2014, a total of 140 oral swab were collected from immunocompromised patients with attending to the three center in AL - Sadder Medical City (Oncology center and kidney center and Center for Diabetes and Endocrinology).In AL - Najaf Governorate, the samples were collected as following : - 110 mouth swabs from patients suffering from leukemia, prostate, stomach and bowel cancer, also 10 mouth swabs from kidney transplant patients and 20 mouth swabs from diabetes. The percentage of female to male was as following, female (86) 64(72.7%) infected, 22(42.3%) uninfected and male (54) 30(57.7%) uninfected, 24(27.3%) infected, with age range between (5 - 64) years. In this study, the results show that the incidence of candidiasis in women was higher than the male also the most cases of candidiasis in patients age range between (45 - 54) years. The results of the present study reveal that the prevalence of oral candidiasis from patient with cancer was more than diabetes & kidney transplant patients which was 72(81.18%), 12(13.65%) & 4(4.54%) respectively. When culturing oral samples on the primary isolation media Sabouroud dextrose agar (SDA) and the secondary media on CHROM agar, The results reveal the presence of 88 isolates belong to Candida spp.which include : - 42 (47.68%) belong to Candida albicans, 20 (22.72%) belong to Candida. dubliniensis, 18 (20.5%)belong to Candida krusei, 6 (6.8%) belong to Candida glabrata & 2 (2.3%) belong to Candida parapsilosis. According to this result, Candida albicans was found the most frequently isolated species and the occurrence of the other non - albicans isolates such as Candida dubliniensis and which followed Candida albicans in frequency of the isolates in oral candidiasis. In this study, the ability of C.albicans & non - albicans was tested to produce some virulence factors such as production of enzymes phospholipase, lipase, germ tube, chlamydospore & biofilms when culturing Candida spp. on different media. The results of the culture show that Candida albicans produces all virulence factor included germ tube, chlamydospore, biofilms & phospholipase. Some species share several phenotypic characteristics ability to produce enzymes of phospholipase such as C.krusei while C.dubliniensis produce chlamydospore, when culturing in the Casein agar & produce biofilms, In addition the molecular tests to was used for Candida albicans confirm the ability to secreted aspartyl proteinase (ASP2). This study showed the amplified from the Candida spp. as following : - C.albicans 306 bp, C.glabrata 594 bp, C.parapsliosis 387 bp, C.krusei 651 bp & C.dubliniensis 451 bp.

عزل وتشخيص بعض فطريات الاذن الخارجيه واختبار قدرة الفطر Aspergillusعلى افراز بعض انزيمات عوامل الضراوة == Isolation And Identification of Some Fungi of Otitis Externa And Testing The Ability of Aspergillus Spp. To Produce Some of Virulence Factors Enzymes

Author name: وفاق احمد محمود

Supervisor name: سميرة كاظم حميد | ياسر لفتة حسون

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

Abstract: جمعت (113) عينه سريريه (مسحة اذن Ear swab) لمرضى التهاب قناة الاذن الخارجيه الفطري Otomycosis من كلا الجنسين والذين راجعوا استشارية الانف والاذن والحنجره في مستشفى الصدر التعليمي والعيادات الخاصه للاطباء الاختصاص / محافظة النجف الاشرف للمده من تشرين الثان | This study included 113 ear swabs from patients with otomycosis, males and females, visited the otolaryngology consultation room at Al - Sader Medical City in An - Najaf / Iraq in addition to the private otolaryngology clinics of some of ear, nose and throat specialists, during the period from October 2012 to March 2013, the study aimed to isolate and diagnose the causative opportunistic mycotic infections of the ear and to test sensitivity of these organisms towards antimycotic agents in addition to identifying some enzymes of the most fungal reluctant type phenotypically and molecularly. (103) positive swabs were obtained from total samples including "Cephalosporium, Candida spp, Aspergillus spp, Neurospora and Trichosporium beigelii.The Precentage of isolated fungi was (85.43)% for Aspergillus, (10.67)% for Candida, while other species constituted totally (3.88)%. The study show that the highest percentage of isolated fungi was from males as it was(51.4)% while in females was (48.6)%. Patients' ages were found to be between (11 - 80) years and the age group (21 - 30) years was the most frequent Category in (30.3)% and the Category (71 - 80) years was affected only in (0.8)%. The highest percentage of insulation fungi during the period of sample collection was in February which constitute (30.9)% and the lowest percentage was in March reaching only (14.1)%.The highest ratios to isolate the fungus was recorded among housewives and unemployed males and it was (44.2%), while the lowest rates of insulation when the students reached (15.9%).The cases of otitis media excelled on the rest of the pathological cases accompaniments of Otomycosis as the proportion of insulation was (19.4%), followed by disease of blood pressure which their percentage was (5.3%), while the rest of the pathological cases did not have significant influence in insulation, for more ear side affected the left ear was affected in(51.8)% and the right was affected in (48.2) %. The study showed that there is inhibitory effect of (flucanazole, Nystatin, Clotrimazole, Miconazole, Itraconazole and Ketoconozole) against Aspergillus spp (A.niger, A.flavus and A.terreus). All antifungals were effective and their inhibitory ratio was (100) %, except for the antifungal (Fluconazole ) which found to have a lower inhibitory effect (10) % against the species A.flavus only. Testing the phospholipase activity for aspergillus species, A.flavus and A.niger showed phospholipase activity in two degrees (++, +++), but A.terreus shoed only (++). Searching the gene alp " which is encoded for the production of alkaline protease " showed that all the Aspegillus species produces the gene with amplification results of (100)% for the band supposed to appear at the molecular weight of (747) pb

تقييم بعض جينات المقاومة للمضادات الحيوية في عزلات الكلبسيلا == Evaluation of Some Antibiotic Resistance Genes In Klebsiella Isolates

Author name: زاهد سعدون عزيز

Supervisor name: عباس شاكر جواد المحنة | سلمان عزيز عدوس

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

Abstract: هدفت الدراسة الحالية الى تقييم انتشار جينات البيتا لاكتاميز ? - lactamases ومنها جينات البيتا لاكتاميز الواسعة الطيف lactamases - Extended Spectrum ? وجينات اخرى مثل جينات المقاومة للكوينولونات Quinolones المرتبطة بالبلازميدات plasmid mediated quinolones | The study aimed to evaluate the prevalence of plasmid mediated ? - lactamases including Extended Spectrum ? - lactamases and non - lactamases and study the horizontal gene transfer. A total of 800 of urine samples were taken from patient suffering from urinary tract infections had been collected during a period from February, to September of 2012, from the hospitals of AL - Najaf province. All the samples were cultured on MacConkey agar. From those 300 samples gave positive bacterial growth , 250 were lactose fermentative isolates, which were submitted to conventional tests including IMVIC and motility tests ultimately lactose fermentative, non motile isolates were candidate to Vitek 2 system to confirm the identification. the results revealed that there were 42 (16.8%) of isolates identified as Klebsiellae represented by Klebsiella pnumoniae ssp pneumoniae, 9(3.6%) of isolates diagnosed as Raoultella ornithinolytica, and 1(0.4%) for both K. pnumoniae ssp.ozeanae and Raoultella planticola. Screening tests were performed , disk diffusion test revealed different pattern of resistance, screening of ESBLS by MacConkey agar medium supplemented with 2 mg /l of Ceftazidime showed that 31 (58.5%) of isolates were initially ESBLS producers.Phenotypes confirmatory tests were conducted to different classes of antimicrobial agents, Disk synergism test revealed that 20(37.74%) of isolates were ESBLS positive, while Disk combination test by Ceftazidime + clav and Cefepime + clav revealed that 28(52.83%) and 43(81.13%) were ESBLS producers respectively, disk replacement test pointed that positive isolates were 26(49%), vitek2 system revealed 33(62%) of isolates were ESBLS producers.Imipenem - Ceftazidime antagonism test revealed that there was no isolate produce induced AmpC beta - lactamase, AmpC disc test revealed that no one of isolates were AmpC producer.The result of MHT(Modified Hodge test) revealed that all isolates were not Carbapenemases producers. Molecular study of different antimicrobial resistance genes were performed, the results reveald high percent of occurence as follow : blaTEM genes (90.6%), blaSHV gene(81.13%), blaCTX - M genes (88.6%), sul genes (88.6%), aac(6')Ib - cr genes (84.9%) and qnr - B genes (41.5%).the study also highlighted an association between studied genes. Finally many attempts to study of gene transfer by conjugation were conducted but all of them were failed, except one isolate (No. 5) was succeeded with frequency of conjugation (0.5×10 - 7). This study concluded that there were high prevalence of some plasmid mediated genes of isolates with clear multi gene resistance patterns as compared with some other genes which propose the high selective pressure of these genes and isolates might acquired resistance by mobile elements such as plasmids and integrons.

انتشار المقاومة للمضادات الحيوية وبعض عوامل الضراوة في بكتريا Pseudomonas aeruginosa المعزولة من مستشفيات في النجف المعزولة من مستشفيات في النجف == Molecular Study To Some of Pseudomonas Aeruginosa Virulence Factors Isolated From Clinical And Environmental Isolates

Author name: ذو الفقار حيدر حسن

Supervisor name: ماجدة مالك متعب

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

Abstract: اجريت الدراسة الحالية لغرض التحري عن وجود الجينات الكروموسومية المسؤولة عن بعض عوامل الضراوة المهمة لجنس Pseudomonas aeruginosa كانتاجها للسم الخارجي A والالجنيت اضافة الى الجينات الكروموسومية المسببة لمقاومة الكوينولونات في العزلات السريرية والبيئية لبكت | This study include investigate the presence of chromosomal quinolone resistance genes and virulence genes(toxA and algD) in clinical and environmental isolates from Al - Sadr Medical city.A total of 110 samples were collected from different clinical sources(burns, wounds, and urine), and 50 from the hospital environment.the samples were collected during 10, Oct, 2012 to 2, Feb, 2013. A total number of Pseudomonas aeruginosa isolates accounted for 89 of 160 samples.The distribution of these isolates were 28 from Burns, 14 wounds, 15 urine, and 32 from hospital environments. Identification of isolates has been accomplished depending on phenotypic diagnostic methods and PCR technique.our study proved that PCR technique depending on oprI and oprL genes was a rapid method for identification of non - pigmented isolates. A total of 20 antibiotics resistant isolates were chosen for Molecular studies. The result reveling that 100% of isolates had a chromosomal mutation in the QRDRs of genes (gyrA, gyrB, parC, and parE). High percentage of isolates(45%) carried mutated genes gyrA and parC, followed by 15% of isolates were carried either mutated gene gyrA alone or three mutated genes gyrA, parC, and parE and and 10%of isolates were carried three muted genes gyrA, gyrB, and parC, while gene that encoding to parC mutation were detected in 5% of isolates. Our study is the first study about detection of Quinolone Resistance Determining Regions in P.aeruginosa in Iraq. The results showed the the presence of mexAB - oprM multidrugs efflux system expression in 100%(20) isolates. while the phenotypic test depending on MIC values for ciprofloxacin alone in the presence of efflux inhibitor showed that only 15%(3) isolates had the active efflux pump.

تقييم مستوى بعض الحركيات الخلوية لدى المرضــى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1 == Evaluation of The Level of Some Cytokines In Patients With Herpes Simplex Virus Type I (HSV - 1)

Author name: حسين علي كاظم

Supervisor name: سهام جاسم الكعبي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

Abstract: هدفت الدراسة الحالية الى تسليط الضوء على مجموعة من المرضى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1(HSV - 1) من خلال تقييم مستوى نوعين من الحركيات الخلوية الموجودة في مصولهم. شملت هذه الحركيات الخلوية الانترلوكين Interleukin - 6(I | The present study aimed to highlight a group of patients infected with herpes simplex virus type I (HSV - 1) by evaluating levels of two types cytokines in sera. These included cytokines interleukin - 6 (IL - 6) and tumor necrosis factor - alpha (TNF - ?) has been evaluated using the technique of Enzyme - linked immunosorbent Assay (ELISA), has also been investigating the relationship between these cytokines and the stages of infection compared to the control group. Before that rapid cytologic method has been used to diagnose the virus by detecting the presence of multinucleated giant cells, and then was diagnosed serologically by specific IgM and IgG monoclonal antibodies against Herpes simplex virus - 1. It was also used Radial immunodiffusion method to evaluate levels of total IgM and IgG in patients sera.Sixty four sera samples were collected from patients who are attending to the consultants clinics of Al - Hakeem general hospital and AL - Sader teaching hospital in AL - Najaf AL - Ashraf governorate for the period from October 21/2012 until March 13/2013. These Samples were divided into two groups : (32) sample during primary and recurrent infection, (32) after recovery from infection (latent stage), as well as (16) sample were collected from healthy individuals, who were regarded as acontrol groups.The study reached to the following results : • A significant increment (p<0.05) in sera concentrations of IL - 6 in patients during infection as compared with control groups, but the increment was not significant in sera of patients during latent stage.• TNF - ? levels increased in sera of patient, but this increment was not significant as compared with controls group.• There was no significant increase of Total IgM levels in sera of patients.• Total IgG levels increased significantly in sera of patients during infection and latent stage as compared with control group.• A highly significant positive correlation (r= 0.7, p= 0.00) between TNF - ? and IL - 6 concentration in sera of patients.• There was no significant correlation (p>0.05) between Total IgM levels and (IL - 6 and TNF - ?) in sera of patients with correlation coefficient (r= 0.1, r=0.2) respectively.• Positive significant correlation (r= 0.4, p= 0.02) was found between total IgG levels and IL - 6 in patients during infection, but was no correlation between them in latent stage.• There was no association between age and IL - 6 and TNF - ? levels in sera of patients with HSV - 1.Finally, the elevation of TNF - ? and IL - 6 levels in sera of infected patients with HSV - 1 may be play an important role in pathogenicity of virus, as well as it may limits the viral infection.Furthermore, there are positive correlation between levels of TNF - ? and IL - 6 in sera of patients

دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius == Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius

Author name: ايمان حمزة محمد

Supervisor name: احلام نعيم كاظم الياسين

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

Abstract: هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.

دراسة بعض الجينات المشفرة لعوامل الضراوة لبكتريا Acinetobacter baumannii المعرزلة من العينات السريرية == Study of Some Virulence Factors Encoded Genes of Acinetobacter Baumannii Isolated From Clinical Samples

Author name: عصام محمد طاهر الخويلدي

Supervisor name: مهدي حسين محيل العمار

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Science - Department Of Biology

Language: English

University location: Najaf

First pages:

Abstract: اجريت الدراسة على300عينة سريريه مختلفة حصل عليها من الجروح والادرار والقشع والدم خلال الفترة من ايلول 2013 الى شباط 2014.منها 102(34%) لا يوجد نمو بينما 198(66%) نمت على وسط زرعي متخصص. شخصت عشرة عزلات من Acinetobacter baumannii من مائة وثمانية وتسعون من | Out of 300 various clinical samples obtained from wounds, urine, sputum, blood through the period from September, 2013 to February, 2014 of which 102 samples(34%) yielded no growth while 198 samples (66%) were positive growth on selective media. Ten isolates of Acinetobacter baumannii were identified among one hundred ninety eight of Gram - negative bacteria grown on MacConkey agar that was isolated from different clinical specimens in Al - Najaf Al - Ashraf province hospitals. A.baumannii identification depends on morphological, microscopic examination and biochemical tests as the initial identification. Also it was identified by both blood agar containing D - glucose and growth at 44?C. The final identification was performed by Api20E to confirm from all A.baumannii isolates..Distribution of A.baumannii among various clinical specimens where 4 isolates (40%) of wounds specimens and 3(30%) of urine specimens, followed by 2 isolates(20%) and one isolate(10%) of sputum and blood specimens respectively.The virulence factors of A.baumannii were studied, including bioflim formation, adhesion, capsule, hemolysin and protease, beta _lactamases production. The results indicate that 60% of A.baumannii formed the biofilm, 90% of isolates have ability to adhesion to epithelial cell and 70% of isolates was the capsule production, 80% of A.baumannii isolates able to produces beta _lactamases while all A.baumannii isolates cannot produce of both hemolysin and protease.The results showed variation in the resistance of these bacteria to antibiotics where all A.baumannii isolates 100% resistance to each of cefotaxime, Ceftazidime, ceftriaxon, piperacillin while 90% resistance toticarcillin - clavulanic acid, 50% resistance to tobramycin and 30% to ciprofloxacin. 20% of all isolates showed resistance to each of amikacin, Doxicycline, imipenem, meropenem.By using Combing Disc Test(CDT) method to detect Extended Spectrum ? - lactamases(ESBLs) and metallo ? - lactamases (MBLs) production.Where 90% of A.baumannii showed ability to produce of ESBLs while only 20% showed ability to MBLs production.The genotypic method was used to detect csuE, ompA genes and also some of ? - lactamases genes such as blaTEM, blaSHV, blaNDM - 1 by using Polymerase Chain Reaction technique.The results showed that 60% of all A.baumannii isolates produce phenotypic biofilm and 30% carry csuE gene of all isolates while 50% when compared with isolates that show phenotypic formation of biofilm and the distribution of gene among all clinical specimens where 50 %, 33.3% and 25% in sputum, urine and wounds specimens respectively while not present in blood specimens. The results showed that 40% of all A.baumannii isolates carry ompA gene while 44.4% when compared with isolates that show phenotypic adhesion and the distribution of gene among all clinical specimens where 100%, 50 %, 33.3% and 25% in blood sputum, urine and wounds specimens respectively.The outcomes showed 70 % of A.baumannii isolates carry blaTEM gene and 40% of A.baumannii isolates carry blaSHV gene while none carry of blaNDM - 1gene. When compared with phenotypic results of ESBLs, MBLs.The results of blaTEM, blaSHV genes become 77.7% and 44.4% respectively.