البحث — المستودع الرقمي العراقي

المستخلص: شملت الدراسة جمع 120 عينة اخذت من مصدرين مختلفين, 60 عينة منها جمعت من بيئة مائية هي نهر الديوانية, بينما كانت 60 عينة سريرية جمعت من الاشخاص الراقدين والمراجعين لمستشفى الديوانية ومن الاصابات الجلدية المختلفة التي شملت الدمامل, الحصف, التقشر الجلدي, الخر | The study included assemble 120 samples which had been taken from two different sources, 60 samples collected from An aqueous environment which is Al diwaniya river, where the rest 60 samples have been collected from those people who were admitted to the Al diwaniya hospital and from of different ages from male and female. Where the environment examples collected from different places of riverbed Al diwaniya. The duration of collection samples has lasted for 3 months, started One handrad isolated had been diagnosed that proved its belong to a bacteria Staphylococcus aureus, 50 isolated where taken from environment isolate and the other 50 taken from clinical isolate depending on the diagnostic methods which included tests of farm, microscopic and biochemical, It was also confirm the diagnosis of bacterial using accurate diagnosis that uses device VITEK System tested sensitivity of bacteria isolate of S. aureus, against 12 selected antibiotic, environment and clinical cases has shown resistance and sensitivity of different varied by source isolate, the nature of the antibiotic and the resistance and sensitivity have been determined by measuring diameters of areas inhibition around tablets of antibiotics it used.All the clinical isolates showed a complete resistance for the antibiotic Lincomycin with 100% percentage, where it's resisted Pnicillin G with 92%, Nalidixic acid with 42%, while the highest resistance of environment isolates was 97% for Lincomycin. The clinical isolates showed a sensitively against the two antibiotics which are Cefamandol and Cephalothin, where their percentages reached 98% and 89%, respectively. Where the percentage of environment isolates reached for the same antibiotics 100% and 97% respectively. In general, the clinical isolates were more resisted for the antibiotics than environment isolates in overall rate.differed values of minimum inhibitory concentrationsmic MIC and the minimum bactericidal concentration MBC among each other with variation of clinical isolates from side and differed with environment isolates from another side while the values differed with environment and clinical isolates from third side, where the highest value reached of MIC against Polymyxin B is (20 - 120) and (100 - 240) mg/ml for environment and clinical isolates respectively, while the highest value of MIC that also was against Polymyxin B reached (40 - 140) and (130 - 250) mg/ml for environment and clinical isolates respectively. Where the value of MIC against Cephalothin reached (0.01 - 0.6) and (0.8 - 2.0) mg/ml for environment and clinical isolates respectively. Finally, the highest value of MBC also was against Cephalothin reached (0.08 - 0.9) and (0.9 - 3.0) mg/ml respectively. In general, the bactericidal and inhibitory concentrations of the clinical isolates are higher concentrations from those bactericidal and inhibitory concentrations for environment isolates.I studied the virulence factors that come from strains of S. aureus bacteria environment and clinical which included Capsula, enzymes of Coagulase that are associated and free, , Lipase, Hyaloronidase and B - Lactamase in addition to Haemolysin clinical isolates and Leucocidin and all the environment isolates are the highest percenage in the ability to form and possess virulence factors compared with environment isolates, where the highest percentage to produce virulence factors is belongs to the Haemolysin which reached 94% percentage in the clinical isolates where in the environment isolates reached 64% percentage followed by Leucocidin that reached 84% percentage in the clinical samples and 40% percentage in the In environmental samples.Also the percentage of blood Coagulation enzyme the associated and free is the highest percentage in the clinical isolates which reached percentage 42% and 38% for the associated and free respectively, where the percentage in environment reached 20% and 36% respectively and the virulence factors was the least presence in the environmental and clinical isolates is the portfolio that reached percentage of presence 22% and 12% respectively.

تاثير اشعة الفا على التصاقية بعض انواع البكتريا == Study The Effect of The Alpha Ray On The Adhesion Some Type of Bacteria

اسم المؤلف: فردوس غضبان كاظم

اسم المشرف: زياد متعب الخزاعي

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية - البكتريا

الدرجة: دبلوم عالي

الجامعة: جامعة القادسية - كلية التربية - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: تضمنت الدراسة الحالية جمع 37 مسحة من المرضى المصابين باخماج العيون في مدينة الديوانية ومن كلا الجنسين حيث تضمنت الدراسة سيادة نوعين من المسببات البكتيرية لاصابات العيون ودراسة قابلية التصاق هذه البكتريا على العدسات اللاصقة وهي المكورات العنقوديـــــــــــ | The current study included (37) samples were collected from sicks of Eyes infection in Al - Diwaniya city from two kinds that the study included two type dominant of Bacteria causes for Eyes infection and study this Bacteria adhesion ability on the Lenses, that the Staphylococcus aureus and Pseudomonas aeruginosa as well as the impact of exposure to (Radiology Alpha and Gamma rays) on adhesion ability, As well as the search included the application of a mathematical model to adhesion formula. The current study results are showed the Staphylococcus aureus for percent (45.94)% and then the Pseudomonas aeruginosa for percent (32.43)%. The results were obtained being clear impact of irradiation on Bacteria adhesion ability to Lenses. The results were obtained the higher level adhesion reaching its, the Staphylococcus aureus in the natural case to (cfu/mm2 10 - 6×292) that in three hours whereas the Alpha ray exposure more than adhesion level that range six hours to (cfu/mm2 10 - 6×302) and when the Gamma ray exposure the higher adhesion level reaching that range (cfu/mm2 10 - 6×233) during exposure period that reach to four and half hour. About the Pseudomonas aeruginosa the adhesion higher level in the natural case to (cfu/mm2 10 - 6×204) during two hours and half hour whereas when exposure its, to Alpha ray that reaching to higher level adhesion range (cfu/mm2 10 - 6×298) during six hours as well as when exposure to Gamma ray the higher level adhesion reaching range (cfu/mm2 10 - 6×250) during four and half hour. Application the mathematical adhesion sample, the result obtained that the disengagement coefficient, the valuable impact its, so that the valuable increased as well as temporal to be late in approach stage of zero who mathematically certain the lenses importance that the important dangerous effective for Eye infection distribution. As well as the results were certain the Alpha and Gamma rays were irradiation, that have impact of where decreasing the adhesion bacteria numbers for lenses, that decreasing from Eyes infection