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مقارنة عزلات مختلفة من الفطر Rhizoctonia solani Kühn باستخدام تقنيات الوراثة الجزيئية وبعض المعايير المظهرية والفسلجية

اسم المؤلف: علاء محمد رشا عبد الامير الرديني
اسم المشرف: محمد عامر فياض | طالب احمد جايد
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - الامراض
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This study had been carried out at Plant Protection Department and Genetic Engineering Labs of Agricultural College / Basrah University, while pots experiments were carried out at fields of Agricultural College / Basrah University. The present study included a comparison experiments of different Rhizoctonia solani isolates depending on molecular genetic techniques alongside with morphological and physiological parameters, host range and anastomosis groups identification.Thirty two different isolates of R. solani have been isolated from several host plants from several plant families throughout different geographical areas at Basra governorate. Result of pathogenicity test revealed that the majority of R. solani isolates caused a significant reduction in the germination percent of cabbage seeds, the isolates of Rs5, Rs16 and Rs19 were found to be more pathogenic and led to complete inhibition of germination, hence, the other isolates varied in their effect on seed germination percent and reported the range of 3.3 - 66.7 %, while, the isolate of BRs15 (binucleate isolate) did not show any significant effect on seeds germination compared to control (untreated) treatment (100%). According to pathogenicity test results, the most pathogenic isolates (Rs2, Rs5, R9, Rs16, Rs27 and Rs29) were selected for more followed experiments.Morphological analyses of R. solani isolates elucidated a profound difference among these isolates, the colony color was dark brown with the Rs9 isolate, light brown at the Rs16, while it was cream at the Rs2, Rs5, and Rs27, a white color with light brownish color was observed with the isolates of Rs29. The growth patterns at the medium supplemented with Potassium Chlorat showed that the isolates of Rs5, Rs9, Rs27 and Rs29 were sensitive to chlorate, while, the isolates of Rs2 and Rs16 were resistant to Chlorate. Regaling the effect of temperature on the growth of R. solani, it was found that the optimal growth temperature was at 25 °c for all examined isolates, while no growth was observed for all isolates at the temperature of 10 and 40 °c. The best pH values for growth supporting were 6 and 7 for all examined isolates.Anastomosis group analysis revealed the occurrence of anastomosis among the isolates of Rs5, Rs16, Rs27 and Rs29 with the standard isolate AG2, this confirms that these isolates belong to the group AG2, while the anastomosis between the two isolates Rs2 and Rs9 with standard isolate AG2 did not happen, therefore these isolates belong to a different group. Microscopic observation revealed theBoccurrence of more than two nuclei in each cell of examined isolates the average of nuclei number was 4 - 8 nucleus / fungal cell, while the number of binucleate isolate BRs15 exhibition two nuclei / fungal cell.The DNA extraction experiments for R. solani isolates (Rs2, Rs5, Rs9, Rs16, Rs27, Rs27, Rs30 and AG2) revealed the efficiency of CTAB procedure to get a high DNA yield and that it’s a good quality, as indicated results of measuring the concentration of DNA isolates it was good quantity amount (197.9 - 560.4 ng / μl) was obtained by following this procedure. The random amplified polymorphic DNA (RAPD) results showed that all DNA of examined R. solani isolates were amplified by employing different RAPD primers, also each genetic primer succeeded in differentiation among isolates, as showed the polymorphic bands and monomorphic bands.The results of restriction fragment length polymorphism technique (RFLP) using RS1 and RS4 primers showed that the bands size for R. solani isolates obtained between 500 - 522 bp, restriction technique results using enzymes AvaII, HincII, MseI and MunI showed that Rs2 isolate belong to the group AG1 - IB, while Rs5, Rs16, Rs27, Rs29 and the standard isolate (AG2) belong to the group AG2 - 2, while Rs9 isolate belongs to group AG11 so Rs30 isolate belongs to group AG3 - PT.Result of host range of R. solani isolates showed a significant differences in the host preference, the isolates of Rs2 was able to infect all plant hosts except okra plant, while Rs5, Rs16, Rs27 and Rs29 were able to induce the infection on marshmallow, okra, cowpea, cotton and eggplant, no infections were reported on the host of melon and wheat plants, regarding R. solani isolate Rs9 an infection was observed on marshmallow, wheat and cotton, while no infection was noticed on melon, okra, cowpea and eggplant.Results of binucleate R. solani isolates role in protection of cotton plant from the infection with R. solani, revealed their protective role, the pathogenic effect of R. solani was significantly reduced, the germination percents were increased with BRs15 treatment reached 83.3, 86.7, 93.3, 83.3 and 90% at the treatments of Rs2, Rs5, Rs9, Rs16 and Rs27 respectively, without any significant difference with control treatment (93.3%). The highest germination percent was observed at BRs15 treatment with Rs29 and reported the percent of 96.7 %. Similar results were observed with damping - off percent of cotton plant, which proved the significant efficiency of binucleate isolate in their protection against all examined R. solani pathogenic isolates.

تاثير الرش بالجبرلين والمنغنيز في نمو وحاصل ونسبة البروتين لمحصول الماش Vigna radiata L. == Effect of spraying by Gibberellin and manganese on growth yield and quality of mung bean(Vigna radiata L).

اسم المؤلف: علاء صبري فضالة الازيرجاوي
اسم المشرف: كريم حنون محسن
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: النبات - المحاصيل الحقلية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Field experiment executed during 2016 autumn season in farmers' fields in the AL - Aufia district southwest of Missan province ( about 3 km from city center) in Silty loam soil. The objective of the study was to know the effect of sprayed of gibberellin and manganese on growth, yield and protein percentage of Mung bean (Vigna radiata L.).The experiment executed in split - plot in (R.C.B.D) design with three replicates, main plot were include three gibberellin concentration ( 0 , 100and200 mg GA3wq L - 1) , While sub plot were include three concentration of manganese (0, 50and100mg Mn L - 1) the local variety of Mung bean was used, the experiment include 27 experimental units, area of each units(3×3)m.The stude include following characteristics : Number of days from planting to maturity ,plant height(cm), number of leaves(leaf plant - 1), number of branches, leaf area(cm2), leaf area index, number of pod plant - 1, number of seed pod - 1, weight of 100 seed(gm), total seed yield(kg ha - 1), percentage and protein yield in seeds(%).The result of the study showed the following : 1 - gibberellin sprayed with concentration 200 mg l1 - caused significant increasing in most of growth characteristics of mung bean (plant height, number of leaves, number of branches, leaf area,), and most of yield characteristics (number of pod in plant, number of seed in pod ,total seed yield 865.70 kg ha - 1, percentage and protein yield in seeds ,and sprayed of gibberellin non - significant on weight100seed.2 - Sprayed of manganese with 100 mg L - 1 concentration modify total studied characteristics and gave highest average plant height, number of leaves, number of branches, leaf area, leaf area index, number of pod in plant, number of seed in pod , total seed yield 898.76 kg ha - 1, percentage and protein yield in seeds.3 - Results also showed a significant Interaction between gibberellin and manganese, the combination of (200 mg GA3 L - 1×100mg Mn L - 1) gave the highest seed yield 1002.66 kg ha - 1 and protein Yield 268.86 Kg ha - 1,the increase of total seed Yield due to most of growth characterists and Yield components.

تحضير حليب فول الصويا المتخمر ودراسة صفاته الكيميائية والميكروبية والحسية وتاثيره على بعض المعايير الذموية للجرران == Preparing Fermented Soymilk and Studying the Chemical, Microbiological and Sensory Characteristics and its Effects on Certain Blood Criteria of Rats

اسم المؤلف: علاء سعد ناجي الشريفي
اسم المشرف: علي احمد ساهي | علاء كرين نعيمة
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: الاغذية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The current study includes soymilk extraction from its grains with three different extraction methods and study the chemical composition, physical properties of the milk for each method. the second method was chosen because its produce milk includes 10.44% total solid material, the chemical composition consist of moisture 89.56%, protein 4.188%, fat 2.47%, ash 059%, carbohydrate 3.192%, and phytic acid 0.225% while the rate of mineral ions ferric, magnesium, phosphor, calcium and potassium (0.004, 0.1, 0.03, 0.09 and 0.063) respectively. The starter company which consist of Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium sp. were used in soymilk fermentation and the best incubation temperature 40 °C for 7 hours , inoculation volume 5% the log of the total count of lactic acid bacteria 10.74 cfu / gm and the log of Streptococcus thermophilus 8.56 cfu / gm and the log of L. acidophilus 7.86 cfu / gm, the log of Bifidobacterium 7.65 cfu / gm, the pH was 4.42 and the rate of total acid was 0.8%. The cold storage effect for 28 days were studied for probiotic bacteria survival, the log of total bacterial account 9.78 cfu / gm, the log of St.thermophilus 8.07 cfu / gm and the log of Bifidobacterium sp 6.5 cfu / gm, while the log of L. acidophilus account was the most effect 4.1 cfu / gm the pH value decreased for 4.6 while the total acidity percentage was 0.81%.The chemical composition of yoghurt was studied The percentage of each moisture, protein, fat, ash and Carbohydrate were (88.8, 4.2, 2.3, 0.82 and 4.6) respectively, the phytic acid rate decreased for 0.075% The experimental animals were fed with milk soybean yogurt with 3 ml and 2 ml for 40 days , the result showed decreasing the rate of cholesterol and triglyceride in T1 treatment which were( 114.24 , 74.5 mg / deciliter) respectively compared with control (97.78, 124.67 mg / deciliter) the hematocrit, hemoglobin, White blood cells, thrombocytes, red blood cells, and the average quantity of hemoglobin, the size of blood cells and the hemoglobin concentration in T1 treatment were (49.66,14.76, 18.96, 246.3, 6.4, 16.4, 62.33, 30.53) respectively compared with control (42.0, 12.0, 12.6, 206.3, 6.0, 13.53, 45.33, 24.6) respectively. The standard of immunity were investigated for the experimental animals the rate of neutrophil degreased in T1 treatment which was 7.83% while in control 14.72% the rate of lymphocyte increased in T1 treatment 90.62% while degreased in control 82.0%.The sensory evaluation showed that the yogurt produced from soybean milk include garlic taste had the highest degree 86.6% while the yogurt without addition of taste had the lowest value which was 53.7%.

تاثير موعد الزراعة ومسافتها والرش بالهيومس السائل في النمو وحاصل البذور والزيت لنبات الخلة البلدي Ammi visnaga (L.) Lam. وفعاليته التثبيطية لبعض الاحياء الدقيقة == Effect of Sowing Date, Plant Spacing and Spraing with Liquid Humes on Growth and Yield of Khellah Plant Ammi visnaga (L.) Lam. and its Inhibition Activitie on Some Microorganisms

اسم المؤلف: علا عدنان فائز حبيب الجابري
اسم المشرف: عصام حسين علي الدوغجي
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - البستنة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The present study was conducted during the growing season of 2013/2014 in the Medical and Aromatic Plants Unit Field, Agricultural Collage, Basrah University, to study the effect of sowing date, plant spacing and spraying with liquid hummus and their interaction on growth and seed and fixed yield of Khella plant Ammi visnaga (L.) Lam. " Syrian Local " and its biochemical effects.The study Included 12 factorial treatments, which were the combinations of two sowing dates 1/10 or 20/10, three plant spacing 20, 30 or 40 cm and spraying with liquid humus in concentration of 0.75 ml. l. - 1 or without ( spraying with distill water) and their interactions. A Complete Randomized Block Design in a factorial experiment was used with three replicates. The results analyses with SPSS program and Least Significant Differences Test (L.S.D) in level 0.05 was used to compared The most important results may be summarized as follows : - 1. Effect of sowing dates : Plants sown on 1/10 gave a significantly increases in plant height, leaf number. Plant - 1, number of main lateral branches. plant - 1 , earliest in inflorescences appearance, number of inflorescences. plant - 1, total seed yield. plant - 1 (54.3 gm), weight of 1000 seed, seeds productivity. hectare - 1 (4754kg), total fixed oil. plant - 1 (2.9gm), fixed oil productivity. hectare - 1 (309.5 kg), leaf total soluble carbohydrates content, seed total soluble carbohydrates content. Whereas plants grown on 20/10 had a significantincreases in dry matter percentage, fixed oil percentage ( 8.3%) and seed total protein percentage.2. Effect of plant spacing : Plants cultivated at 40 cm a part had a significant increases in leaf number. plant - 1, earliest in inflorescences appearance and number of inflorescences. plant - 1. Whereas plants cultivated at 30 cm a part had a significant increases in weight of 1000 seed and leaf total soluble carbohydrates content, Plants cultivated on 20 cm a part gave a significant increases in seeds and fixed oil productivity. hectare - 1 (3543kg and 311.2 kg), respectively.3. Effect of spraying with liquid humus : - Plants sprayed with liquid humus had a significant increases in leaf number. Plant - 1, dry matter percentage, number of inflorescences. plant - 1, total seed yield. plant - 1 (34.0 gm), seeds productivity. hectare - 1 (3543kg), fixed oil percentage (8.1%), total fixed oil. plant - 1 (2.6gm), fixed oil productivity. hectare - 1 (267.2 kg) and leaf total soluble carbohydrates content.4. Effect of the interactionsThe Interactions between the study factors, showed a significant effect in all studded parameters except leaf total chlorophyll content.5. The activity of the Khella seed and leaf extracts against certain types of pathogenic bacteria to human.The activities of aqueous, methanolic and ethanolic extracts of either leaves or seed were tested against some types of bacteria Escherichia coli, Pseudomonas aerugnesa and Staphylococcus aureus at fiveconcentrations 50, 100, 200, 300 or 400 mg. L. - 1. The aqueous extract of the seeds showed inhibition effect in all studied concentrations, the effect increased with the concentration increases.6. The activity of the Khella seed and leaf extracts against two pathogenic fungi Aspergillus nigerCandida albicanThe activities of aqueous, methanolic and ethanolic extracts of either leaves or seed were tested against two fungi Aspergillus nigerCandida albican at five concentrations 50, 100, 200, 300 or 400 mg. L. - 1. All the extracts showed inhibition effect in growth diameters of both fungi, the methanolic extract of leaves was the more effected

مسح وتشخيص بعض الملقحات الحشرية ومصادر حبوب اللقاح في محافظة ميسان == Survey and Identification Some Pollinators and Pollen Resources in Missan Province

اسم المؤلف: عقيل عبد الرزاق كريدي
اسم المشرف: طه ياسين مهودر العيداني | خالد عبد الرزاق فهيد
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - الوقاية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: survey and identification of the most important pollinators were conducted in three locations in Missan province (Amarah, Maymonnh , Almajar alkabir) , for the purpose to survey and identify the most important pollinators in the province,In view of the diversity of plants and insects pollinators the study select three plants , ber tree Ziziphus spina - christi and two crops are alfalfa Medicago sativa and Egyptian clover Trifolium alexenderianum to confine the pollinating insects. Honey bee Apis mellifera be one of the most important pollinator . So pollen sources in the sediment of honey and propolis is studied to identify botanical origin that visited by insects to keep them and provide fingerprint about plant diversity in the province.The study showed the following : 1. there are many insects visiting flowers of ber , alfalfa and clover were some of them are classified to the species level, while others were classified to the generic level such as wild bees Andrena sp. , Megachle sp., Tetralonia sp.And some species belong to family syrphidae such as Eristalis sp. , Syrtta sp. and Syrphus sp..2. Honey bee Apis mellifera is the most common and attracting to the two experiment crops flowers . where on average of 0.978 insects per 3m² during five minutes in alfalfa and 1.2 insects in clover were recorded. the second important is the wild bee Andrena sp. with an average of 0.662 insects in alfalfa and 0.8 in clover. 3. Honey bee were holding maximum quantity of pollen from alfalfa and clover . The average load was 67874 Pollen Grains (PG) was recorded . This quantity may be greater when it's estimated in the bee when they returns to hive .Wild bee Andrena sp. loud of pollen grain counted 54233 PG and ,IIthe wild bee long horn Tetralonia sp. carry about 51664 PG , while the leaf cutter wild bee Megachile sp. and white - banded digger Amegilla quadrifasciata carry 44256 and 38154 PG respectively , The least loud was carried by syrphid fly Eristalis aeneus was 27657 PG in it's gut .4. Honey bee Apis mellifera was the most insect spending time of foraging pollen on clover in average 9.76 second and 8.85 second on clover and alfalfa flowers respectively in the daylight , the next were the miner bee Andrena sp. spending 5.53 sec and 4.32 sec on clover flowers and alfalfa respectively . The white - banded digger wild bee A. quadrifasciata and the leaf cutter wild bee Megachile sp. have not seen visiting clover while they visit alfalfa flowers .5. pollen spectrum in spring and autumn honey sediment was studied , wich showed an important and new sources the tamarisk Tamarix brachystachys that is record the first time as a predominant pollen source , this source rated over than 45% in three sample of spring honey while as known between researchers and bee keepers that spring honey called a Eucalyptus or clover honey. Some weed were also important as pollen sourcessuch as Capparis spinosa , Sonchus oleraceus in addition to Prosopis juliflora and some species of the Families Cruciferae , Compositae and Umbelliferae.6. the light microscope showed that The most important resource of propolis Eucalyptus camaldulensis , Tamarix spp. and Prosopis juliflora

مقارنة القيمة الغذائية لاسماك الكارب الشائع Cyprinus carpio L. من مصادر مختلفة == Comparison of nutritional value of the common carp Cyprinus carpio L. from different sources

اسم المؤلف: عطور عبد الكريم طاهر العطبي
اسم المشرف: امجد كاظم رسن | صلاح مهدي نجم
الموضوع العام: علوم الزراعة
السنة: 2017
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The current study was carried out during the period from December 2015 and June 2016 to assess the chemical composition, fatty and amino acids and the total bacterial count of the muscular tissue from the common carp Cyprinus carpio L. in Basrah city which were cached from Shatt Al - Arab river at Abul - Khasib, obtained from earthen ponds at the University of Basrah, fish cages in Shatt Al - Arab river at Al - Hartha district and those imported from Iran Republic which were available in the domestic markets of Basrah city.The results of the study were statistically analyzed using CRD design applying SPSS statistical package ver. 17 on 50 fish from each source with 3 replicates per sample and the studied factors were tested using RLSD test at 0.05 probability level.The results of the statistical analysis indicated no significant differences (p>0.05) in moisture content between Shatt Al - Arab samples, imported samples, iced pond fish and iced cage fish. No significant differences (p>0.05) were detected also between fish from ponds and cages. Significant differences (p<0.05) were detected between fresh fish from ponds and cages with fresh and iced fish from Shatt Al - Arab or imported fish. The highest moisture content of fresh fish was 78.2% in Shatt Al - Arab samples while the lowest 70.8% was in fresh cage fish. The highest moisture content in iced fish 79.4% was in imported samples while the lowest 74.1% was in cage fish. As for ash content, the statistical analysis revealed significant differences (p<0.05) between imported fish and other fish samples. Significant differences (p<0.05) were observed also between fresh pond fish and other samples.However, no more significant differences (p>0.05) were detected between other fish samples. The highest ash content in fresh fish 2.29% was in pond fish and the lowest 0.98% in imported fish. Similarly, the highest ash content in iced samples 1.45% was in pond fish and the lowest 0.87% in imported samples. The statistical analysis of fat contents demonstrated significant differences (p<0.05) between Shatt Al - Arab fresh and iced samples and other samples. Fat content in pond fish was in line with those in iced cage and iced imported fish but significantly (p<0.05) differ from those in fresh pond and imported fish while no such differences were shown between pond and imported fish. The highest fat content in fresh fish 11.9% was in imported fish and the lowest 3.43% in Shatt Al - Arab fish. Similarly, the highest fat content of iced fish 9.99% was in imported fish and the lowest 3.17% in Shatt Al - Arab fish. The statistical analysis showed that protein contents in fresh fish from Shatt Al - Arab, ponds and cages were significantly (p<0.05) higher than those in imported fish while protein contents in fresh and iced imported fish were significantly (p<0.05) lower than it in other fish. The highest protein content in fresh fish 18.71% was in pond fish and the lowest 10.05% in imported fish. Similarly, the highest protein content in iced fish 16.96% was in pond fish and the lowest 9.98% in imported fish. The statistical analysis showed that the caloric values of fresh and iced fish from Shatt Al - Arab were significantly (p<0.05) lower than other fish except for iced pond and imported fish. Additionally, no significant differences (p>0.05) were observed in caloric values between pond, iced cage and imported fish while pond and Shatt Al - Arab fishes only showed significant differences (p<0.05) in comparison with other samples. The highest caloric value for fresh fish 165.62 Kcal/100 g in cage fish and the lowest 98.5 Kcal/100 g in Shatt Al - Arab fish. On the other hand, the highest caloric value for iced fish 148.4 Kcal/100 g was in cage fish and the lowest 93.9 Kcal/100 g in Shatt Al - Arab fish.The fatty acids was assessed in the laboratories of The College of Agriculture, University of Basrah by using GC - MS QP210 Ultra , SHIMADZU, JAPAN equipped with capillary column DB - MS 5 with static phase (5% phenyl, 95% methyl polysiloxane) and ultra - high purity Helium 99.9%. The fatty acid analysis for the muscular tissue of fish indicated high levels of oleic ،palmitic ،stearic ،stearic ،docosahexaenoic acid in comparison with lower levels of myristoleic ،eicosadienoic ،dihomo - gamma - linolenic and pentadecyclic acids. The statistical analysis revealed no significant differences between both fresh and iced samples from the different fish sources. However, significant differences (p<0.05) were detected between imported fish and fresh and iced fish from ponds and cages. Additionally, no significant differences (p>0.05) were observed between fresh and iced imported samples with both fresh and iced pond fish while fresh and iced cage fish differed significantly (p<0.05) from fresh pond samples.Amino acids were assessed by using HPLC system with ion exchange and Ninhydrin post column derivation in Shimadzu Spd - 6 Av uv - Visible detector. The analysis of amino acids in fish muscular tissue demonstrated high levels of Aspartic ،Serine ،Glutamic،Alanine and Glycine and low levels of Cystine ،Tryptophan ،Isoleucine ،Methionine،Histidine ،Threonine and Tyrosine. The statistical analysis showed no significant differences between fresh and iced Shatt Al - Arab samples with fresh and iced imported fish. No significant differences (p>0.05) were observed also between fresh and iced pond fish with fresh and iced cage fish. On the other hand, significant differences (p<0.05) were found between fresh and iced Shatt Al - Arab fish with fresh and iced imported samples. Additionally, another significant differences were detected between fresh and iced pond fish with fresh and cage fish.As for the total bacterial count, the statistical analysis revealed significant differences (p<0.05) between all fresh and iced fish samples. The highest level in fresh fish 7.6 x 103 cfu/g was observed in Shatt Al - Arab fish while the lowest level 2.0 x 103 cfu/g in imported fish. In comparison, the highest level in iced fish 7.44 x 107 cfu/g was also in Shatt Al - Arab fish but the lowest level 5.72 x 107 cfu/g in cage fish.

عزل وتشخيص بكتيريا . Vibrio spp من بعض الاسماك المتداولة في الاسواق المحلية لمدينتي البصرة والناصرية ودراسة بعض خواصها == Isolation and Identification of Vibrio spp. from some fish proffered in local markets of Basrah and Nasiriyah city and study some properties

اسم المؤلف: عذارء عودة حسين الجبوري
اسم المشرف: خديجة صادق جعفر الحسيني | منال بادي صالح التميمي
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الاغذية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The study included isolation and identification Vibrio spp. bacteria that taken from fresh and frozen fish (Tenualosa ilisha , Hypophthalmichthys molitrix, Barbus sharpey and Spondyliosoma cantharus). 210 fishes were collected from Basrah market (Big market in Basrah, Al - Ashar, Al - Tanoma and 5 mile) while 150 fishes were collected of Hypophthalmichthys molitrix, Barbus sharpeyand Spondyliosoma cantharus) from Nasiriyah market (Big market and Harj market) this collecting process of samples took a period from 02.June till 30.November - 2013.Fishes were brought to laboratory and samples were taken from it and planted directly on biological media (Nutrient agar, Blood agar, MacConkey agar and selective medium (Thiosulphate Citrate Bile salt Sucrose Agar TCBS) then the petri dishes were incubated aerobically at temperature 37cº for 18 - 24 hours. 30 positive samples were identified of Vibrios depending on colony shape, cell under microscope, catalase test, oxidase test and motility test, afer that the types were identified depending on the biochemical tests and by using API20E kit, which led to identifying two kinds of Vibrios bacteria : Vibrio cholera and Vibrio fluvialis. These two bacteria were isolated from fresh fishes more than frozen fishes.Study observed that the Vibrios isolation percentage of these two bacteria in Basrah market was 26% (15 isolates) while the isolation percentage was 21% in Nasiriyah market (63 isolates).Sensitivity and antibiotics tests were done, results showed that all Vibrios cholera bacteria had 100% resistant against Aztreonem, while some of it had low resistant which was about 45% against Amoxicillin - clavulanic Acid and Nalidixic Acid, all Vibrio fluvialis bacteria isolates had resistant against Aztreonem, Amoxicillin - clavulanic Acid and Nalidixic Acid which was 100%, 75% and 25% respectively, while all VibrioAcholera and Vibrio fluvialis isolates were sensitive 100% against Amikacin, Ampicillin, Chloram - phenicol, Ciprofloxacin, Tetarcycline, Trimethoprim /Sulpha - methoxazole.Vibrio cholera and Vibrio fluvialis bacteria isolates were 100% tolerant for acidity at pH 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0, while Vibrio cholera bacteria was 60% tolerant to the acidity at pH 4.5, and Vibrio fluvialis bacteria was more resistant to the acidity of 20% and 50% tolerant at pH 4.0 and 4.5 respectively.The study showed to the ability of Vibrio cholera bacteria to resist the salinity reached 60% and 100% at concentrations 6% and 7% respectively, but it didn't grow on concentrations 8% and 9%.Vibrio fluvialis bacteria was resistant for the salinity in all the concentrations with different percentages reached to 100% at concentrations 6%, 7% and 40% at concentration 8%, while at concentration 9% there was only one sample had resistant of 20% and it was from frozn sample of Beni fish which taken from Big market in Basrah.All isolates from different sources could grow in 25 and 37 cº and in room temperature 15 cº during doing this experiment, while some isolates of Vibrios bacteria could grow with low density in 5 cº, while it couldn't grow in - 20 cº.By using Real Time (PCR) technology for detecting hemolysim enzyme gene in Vibrio spp., that 27 samples from Vibrio cholera bacteria and 3 samples from Vibrio fluvialis bacteria had this enzyme which analyzes red blood cell (RBC) and considered one of toxins that belongs to Vibrio spp. which has a role in occurrence of disease.

التنوع السمكي وبعض الخصائص الحياتية في الجزء الشمالي لشط العرب وبعض مقترباته == Fish biodiversity and some biological characteristics in the Northern part of Shatt Al - Arab River and some of its reaches

اسم المؤلف: عبد المحسن جعفر عبد الله
اسم المشرف: ساجد سعد حسن النور
الموضوع العام: علوم الزراعة
السنة: 2015
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The aimed of the present study to find out the nature of the qualitative compositionand some biological aspects in the North part of Shatt Al - Arab River and some of its reaches after emergence of many changes in the region and habitats segmenting by Dam construction on Euphrates River in the area between Chibyish and Mudina town and establishment of Qlat - Salih Regulator, reduction of water discharge to minimum limits, entry of alien species on an ongoing basis, so it is necessary to conduct a study to find out the nature of the diversity of fish in the region, and it has shown that the composition of community differs from previous studies revealed that introduced alien small size species and some of uneconomic species were dominate. The samples were caught monthly from the river from December 2013 to May 2015 North of Basrah Province. Several fishing tools were used to collect fishes seine, fixed, draft, cast net and electrofishing. Three stations were selected; the station 1 was in the North of Qurna town about five kilometers North the point which the Tigris and Euphrates River confluence, station 2 at the place of Seweeb River is meeting the Shatt Al - Arab Rive and station 3 at the point that Al - Shafii River which meet Shatt Al - Arab river. Some environmental parameters were measured, namely air temperature ranged from 15ºC in December 2014 in station 3 to 43ºC in the August 2014 at station 2 and 3, while the water temperature from 11ºC in January 2015 for station 1 to 36 ºC in July at station 2. Light penetration revealed 33 cm in April and May 2014 and 132 cm in January 2015 for station 3. Current speed ranged between 0.03 m/s in January 2015 in station 3 and 0.29 m/s in October 2014 for station 1. Salinity ranged from 0.66 g/L in January 2014 for station 1 to 1.46 g/L in February and March 2015 in station 1 and October 2014 for station 2. The pH values tend toward the alkaline, the lowest 7.30 in May 2014 for station 2 and 8.90 measured in February 2014 in station 1. Monthly discharges, average incoming from the Tigris River recorded 35.20 ± 8.47 m3/s in November 2014 to 73.85 ± 17.55 m3/s on February 2014. Lower values of reactive nitrate were recorded 0.69 μ g at. N/L in March 2015 at station 3 and highest 24.32 μ g at. N/L in May for station 2, however the values of reactive phosphate recorded, the lowest 0.0037 μ g at. N/L in March 2015 at station 2, the highest was 0.589 μ g at. N/L in November 2014 for station 1. A total of 29405 individuals was collected during the study period represented 916521g included 33 species eight of them were marine species contain 29 genera belong to 14 families of Osteichthyes, Cyprinids family occupied first degree in the number of species (13) and genera (12), the numerical relative abundance of marine species in the study area was 3.38% and the relative abundance gravimetric was 8.45%. A significant positive correlation was found between number of species and water temperature (r=0. 506). Liza abu, Carassius auratus and Coptodon zillii were the most total numerical relative abundance 30.89, 29.30 and 15.64 % respectively, C. auratus, S. triostegus and C. zillii were the most total weight relative abundance and composing 27.58, 14.27 and 11.64% respectively in the study area. Discharges rates flowing from the Tigris River revealed negative effect with the number of species and the insignificant negative correlation was recorded (r= - 0.109) with discharge monthly rates, and fluctuation relationship was noticed between discharges and ecological indices. However, native species included 15 seasonal species 4 and 14 for rare species. Diversity index (H) ranged between 0.91 in January 2015 at station 3 to 2.05 in March 2015 for station 2, richness index (D) 0.83 in February 2014 at station 3 and 2.79 in March 2015 at station 1, evenness index (J) recorded 0.38 in January 2015 and the highest 0.78 in September 2014 for station 1. The percentage of similarity among stations by Jaccard similarity index (Ss%) showed variation uneven during study months. Cluster analysis of species similarity pointed to clumping in groups controlled by temperature. The Multivariate analysis was (CCA) adopted to clarify the impact of a biotic factors on the distribution of species. Total catches were ranged between 16380 g in December 2013 to 80309g in December 2014. The total average catch per unit of effort was 24.953 kg/hr for seine nets ranged from 0.928 kg/hr in December 2014 at station 2 to 30.271 Kg/hr during January 2015 for station 3. The number of fishing boats in the study area 143 their length between 5 - 7m most of them without an engine. The impact of alien species direct effects during the destruction of nests and reproductive ground, especially eggs which lie on a plant for native species and hybridization between alien and native species, and indirect effects of competition on food, ecological space, environment destruction and aggressive behavior. The length groups of all fish species were recorded, the lowest values were for Alburnus mossulensis, Acanthobrama marmid, Hemiculter leucisculus and Garra rufa, they were 7.3 - 18, 6.3 - 17, 7 - 17 and 7.2 - 17.8 cm respectively, whereas the highest were for Silurus triostegus, Leuciscus vorax and Mastacembelus mastacembelus, they were 10.8 - 72.4, 9.5 - 57.3 and 14 - 55 cm respectively. Faben method used for estimating growth parameters (L∞,K) by applying FiSAT program for C. auratus, Carasobarbus luteus and L. abu, the values of L∞ for three species 38.97, 35.69 and 22.19 cm respectively, and for K 0.29 to the C. auratus and C. luteus and 0.41 for L. abu, the results revealed five years age for C. auratus and C. luteus whereas four years age for L. abu. The present study showed high values of diet overlap between L. abu and Cheon subviridis 09% also high values 90% Oreochromis aureus and Oreochromis niloticus S. triostegus and L. vorax 89%, but it did not reach the competition limit, also high diet overlap 86% and competition was found between C. auratus and C. luteus Absolute fecundity for C. auratus females ranged from 1303 for 8.8 cm total length and 10g weight to51245 egg for 30.1 cm total length and 557g weight, and for C. luteus from 2098 for 13.1 cm total length to 14147 egg for 20.9 cm total length and 131g weight, while in L. abu from 6247 for 9.7 cm total length and 11g weight to 41219 egg for 17.5 cm total length and 63g weight.

تاثير التقليم الورقي والرش بالاثيفون في صفات الثمار الفيزيوكيميائية والفسلجية والتشريحية والحاصل في نخيل التمر Phoenix dactylifera L. صنف الحلاوي117 == Effect of leaf pruning and ethephon spray on fruit physico - chemical , physiological , anatomical and yield of Phoenix dactylifera L., cv. Hil

اسم المؤلف: ظافر ناصر عبود خلف القطراني
اسم المشرف: علي حسين محمد الطه
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - البستنة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This study was conducted in a private orchard at Abi El - Khassib district , Basrah Governorate, during the growing season of 2014 to investigate the effect of leaf pruning at levels of 6 , 9 and 12(leaf . bunch⁻¹)during the onset of pollination and spraying ethephon on bunches at concentrations of Zero , 250 and 500 (mg.L⁻¹) , once, at Kimri stage on fruit physico - chemical , physiological , anatomical characters and yield of date palm ,cv. Hillawi . Results showed that leaf pruning treatmeats of 9 and 12 leaf . bunch⁻¹ recorded significant increases in fruit , pulp fresh weight , fruit size , length and diameter and water content of fruit at Rutab stage, carotene pigment concentration of fruit skin and respiration rate of fruit at Kimri and Khalal stages, ripening percentage at Khalal and Rutab stages, bunch weight of tree at Tamir stage . Leaf pruning treatments of 6 and 12 leaf.bunch⁻¹ increased total chlorophyll pigment concentration of fruit skin at Kimri stage significantly. Leaf pruning treatment at 12 leaf.bunch⁻¹ recoreded significant increases in cell length and width of inner mesocarp of fruit at Khalal stage, and total yield of tree at Tamir stage. Treatments of 6 and 9 leaf.bunch⁻¹ increases significantly total chlorophyll pigment concentration of fruit skin and number of cells in mm² of inner mesocarp of fruit at Khalal stage, whereas treatment at 6 leaf.bunch⁻¹ increased fruit dry matter percentage and total acidity and respiration rate of fruit at Rutab stage. Ethephon treatments at concentrations of 250 and 500 mg.L⁻¹ had significant increases in fresh weight of fruit at Rutab stage, and respiration rate of fruit at both Kimri and Khalal stages. Treatment of 500 mg.L⁻¹ ethephon recorded significant increases in pulp weight of fruit , fruit volume , length , diameter , total soluble solides , phosphorus concentration of fruit at Rutab stage, and carotene pigment concentration of fruit skin at Kimri , Khalal stages, and ripening percentage of fruit at Khalal and Rutab stages, and cell length and width in inner mesocarp of fruit at Khalal stage, and bunch weight and total yield of tree at Tamir stage. Both treatments of Zero and 250 mg.L⁻¹ ethephon recorded significant increases in total chlorophyll pigment concentration of fruit skin at Kimri and Khalal stages, and number of cells in mm² of inner mesocarp of fruit at Khalal stage, whereas control treatment of ethephon increased fruit total acidity and respiration rate significantly at Rutab stage. Bi - combination of leaf pruning at 12 leaf .bunch⁻¹ and spraying ethephon at concentration of 500 mg.L⁻¹ recorded significant increases in most of the studied characters as compared to other bi - combination treatments

دراسة بعض الجوانب الحياتية والبيئية للروبيان الشرقي Macrobrachium nipponense (De Haan, 1849) وطرائق تربيته واكثاره في البصرة، العراق == Study of Some Biological and Ecological Aspects, Aquaculture and Breeding Methods of Oriental Prawn Macrobrachium nipponense (De Haan, 1849) In Basrah, Iraq

اسم المؤلف: طارق حطاب ياسين المالكي
اسم المشرف: مالك حسن علي | امنة علي هاشم
الموضوع العام: علوم الزراعة
السنة: 2015
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية انشاء مفقس للروبيان الشرقي Macrobrachium nipponense وتجربته عمليا على الاناث الحاملة للبيض والمصطادة من منفذ المسحب خلال شهري حزيران وتموز لسنة 2013 واجراء عملية التكاثر وتسجيل اربع مراحل تطور جنينية في البيض، وتراوحت مدة التطور الجنيني للبيض بين (20 - (30 يوما.بينت الدراسة تسجيل افضل معدل وزن لليافعات في اثناء تربيتها في الاحواض المختبرية والمغذاة على عليقة ذات محتوى بروتيني 42 % مع نسب البقاء مرتفعة بين 90) - (95%، وهذا ما انعكس على التركيب الكيميائي لجسم الروبيان بعد التجربة. بينت نتائج السلوك بان الروبيان المدروس يفضل التعلق ضمن عمود الماء، اذ بلغ افضل معدل وزن عند استعمال عليقة الرقائق (Aquafin) ونسب بقاء 95 % خلال مدة التجربة، واظهرت نتائج تجارب الاقلمة الملحية واستهلاك الاوكسجين بان التركيز الملحي نصف القاتل (LC50) لليافعات خلال 96 ساعة كان 15.5 psu مع نسب بقاء مرتفعة في الملوحتين (0.9 و(5.0 psu، ولوحظ ارتفاع تدريجي باستهلاك الاوكسجين مع ارتفاع كل من درجة حرارة الماء والملوحة. بلغت نسبة الجنس 1 : 3.8 ذكور : اناث على التوالي. تراوح عدد البيض للروبيان المصطاد من منفذ المسحب بين 3,600) - (10,200 بيضة /انثى. في حين بلغ معدل التفقيس لليرقات (1632 ± (861 يرقة زؤيا، وكان معدل عدد اليرقات نسبة الى وزن الجسم 225 ± 110 /غم من وزن الجسم، كما لوحظ وجود ارتباط بين وزن الامهات مع كل من وزن البيض وعدد اليرقات الفاقسة، واظهرت نتائج رعاية اليرقات حديثة الفقس في انظمة الاستزراع المختلفة بان اغلب اوزانهواصل الى 3 ملغم خلال المدة الممتدة بين 12) - (22 يوما ، وكانت اعلى معدلات نسب بقاء لليرقات 63.75 % سجلت في الحوض الطيني فيما تراوحت بين 27.50) - (47.50% في الانظمة الاخرى.بلغت معدلات الاوزان النهائية (813 و978) ملغم لليافعات الفاقسة المرباة في الاقفاص الطافية والغاطسة على التوالي، في حين بلغت معدلات الاوزان 805) و950) ملغم لليافعات الطبيعية المرباة في الاقفاص الطافية والغاطسة على التوالي خلال 28 يوما، وبنسب بقاء بين (86 - 93)%، في حين تراوحت معدلات اوزان اليافعات المستزرعة في النظام المغلق من (938 والى 4010) ملغم خلال 90 يوما، وبنسب بقاء بين 86) - (92 %، وسجلت اعلى نسب البقاء ليرقات الروبيان ويافعاتها المرباة خلال 42 يوموابلغت 78.13% في الحوض الطيني فيما تراوحت بين (47.50 - (58.13% في الانظمة الاخرى. تحقق افضل معدل اوزان لليرقات المغذاة على عليقة (46% بروتين) ولمدة ستة اسابيع في الحوض الطيني وكان697 ملغم واقلها في الحاضنات وبلغ 312 ملغم، تتحول اليرقات حديثة الفقس (طور الزؤيا) الى طور ما بعد اليرقي بعد عمر 12) - (22 يوما، في حين طور اليرقة المتقدمة بعد عمر 23) - (44 يوما يتحول الى الطور اليافع الذي بدوره يتحول بعد عمر (45 - 129) يوما الى الطور قبل البالغ الذي بعد عمر 130 - 179 يوما يصل الى الطور البالغ، اي بعد عمر 180 يوما الروبيان يصبح في الطور البالغ.حددت انتاجية المفقس من الاحواض الداخلية بين (53,504 - (190,000 يرقة زؤيا، في حين ينتج بين (32,355 - (99,560 يرقة متقدمة، وتراوح الانتاج من الاحواض الخارجية ما بين (4,347 - (24,098 يرقة زؤيا، وبين 2,527) - (18,828 يرقة متقدمة، كذلك على ضوء نتائج المساحة الكلية للحوض الطيني وحوض الالياف الزجاجية فان عدد يرقات الزؤيا التي يمكن ان تستوعبها تتراوح ما بين 36,559) - (15,300,000 يرقة زؤيا، وبين (21,248 - 11,954,400) يرقة متقدمة، اما مجموع الانتاج الكلي في جميع انظمة الاستزراع الداخلية والخارجية فكان ( 12,600,188 - 15,760,462) يرقة زؤيا، وبين 9,753,349) - (12,197,630 يرقة متقدمة.بلغت النسب المئوية للتركيب الكيميائي لبعض مكونات العليقة المصنعة في الدراسة الحالية والمستعملة في تغذية اليرقات 44.84) و51.27 و2.57) % للبروتين والدهن والرماد على التوالي، في حين بلغت لعليقة مسحوق الاسماك المستعمل في تغذية الامهات واليافعات (40.37 و25.29 و15.13) % للبروتين والدهن والرماد على التوالي. | This study included hatchery construction for oriental prawn Macrobrachium nipponense, a gravid females were collected from Al - Masshab Outlet, Basrah, during the peak of occurrence in June - July / 2013, is for application of breeding process, with recorded four developmental of eggs, with range time of embryonic development stage of eggs between 20 - 30 day. The present study revealed that the best weight for juvenile in laboratory which were feeding on diet contain 42% protein with high survival rates between 90 - 95%, this reverse on the chemical composition of prawn body after the experiments. Showed result behaviour that this prawn preferred to feeing hold on the ponds columns, it reach a best weight by using flake feed (Aquafin), with survival rate 95% during the experimental period. The salinity acclimation and oxygen consumption showed that LC50 for juvenile was 15.5 psu after 96 hours, with high survival rate in tow salinities 0.9 and 5.0 psu. A gradual increasing in oxygen consumption with increasing temperature and salinity clearly proved in this experiment. Sexual ratio was recorded to be 1 : 3.8 for males : females respectively. The number of eggs were collected from Al - Masshab Outlet between 3,600 - 10,200 egg for number of females, while the ranged of all hatching larvae were 1,632 ± 861 zoae. The range of ratio of larvae to the ratio of body weight was 225 ± 110 g from body weight of prawn, and the present study showed that there is a correlation between female weight with both egg weight and number of hatching larvae, by using different nursing systems of newly hatched larvae for 12 to 22 day, and the larvae reached weight to 3 mg., while higher range of survival larvae were 63.75% in muddy pond, in others systems were 27.50 - 47.50 %.The final weight range were reached 813 and 978 (mg) for hatched juveniles that were reared in floating and emerged cages respectively, while the naturally reared Juvenile have weight range between 805, 950 (mg) for nature floating and emerged cages during 28 day, with a survival rate between 86 - 93%, and weight range of juvenile reared in closed system from 938 to 4,010 (mg) during 90 day, with survival rates 86 - 92 (%). High levels of survival rate recorded to be 78.13% for larvae and juvenile of prawn cultured earth in ground pond during 42 day, comparison with 47.50 - 58.13% in others system. The best weight for larvae that feeder on protein diet (46%) for six weeks in ground pond reached to 697 mg while the lowest weight in nursing system reached to 312 mg. The age which zoea needs Metamorphosis zoae between 12 - 22 days to reach post - larvae, were 23 - 44 day for Metamorphosis post - larvae to Juvenile, and this needs between 45 - 129 day for reached to sub - adult with in 130 - 179 day. After 180 day more prawn reached to adult stage.The hatchery production of zoea larvae was fluctuated between 53,504 - 190,000, it and between 32,355 - 99,560 post - larvae. The total production from earth pond (mud pond and fiberglass tank), the number of zoae larvae between 36,559 - 15,300,000 larvae, and between 21,248 - 11,954,400 post - larvae. While the total production in all culture systems were 12,600,188 - 15,760,462 zoea larvae, and between 9,753,349 - 12,197,630 post - larvae.The chemical composition for prepared diet the present study which used for nutritional diet experiment were 44.84 % protein, 51.27 % fat and 2.57 % ash. Fish meal constituents was 40.37 % protein, 25.29 % fat and15.13 % ash

دراسة لمرض لفحة سعف النخيل المتسبب عن الفطر Diplodia phoenicum وبعض الفطريات المرافقة له وامكانية مكافحة المرض كيميائيا واحيائيا == A study of date palm leaflets blight caused by Diplodia phoenicum and some fungi associated with it and the ability of it?s chemical and biological control

اسم المؤلف: ضرغام صباح لفتة باقر العوفي
اسم المشرف: محمد عامر فياض | يحيى عاشور صالح
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - الوقاية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This study was carried out at the laboratories of plant protection Department - College of Agriculture - Basrah university for a.peroid during 1/9/2014 - 27/7/2015 . The study aimed to detect the leaflet bligh disease for different datepalm cultivars such as Chipchap ,Sayer , Zehdi and Khodrawi at three regoins, Medainah , Abu Al - khaseeb and Nashwa.The result showed that the infection percent was 86.51, 83.61 and 76.03% at Medainah , Abu Al - khaseeb and Nashwa respectively . The results also showed that the infection intensity was 25.96 , 23.56 and 21.1 % for the three above regoins respectively .The higher infection intensity was noticed on Chipchap culture which was 30% while the lowest one found on Sayer culture which was 23.3% .The infection intensity also differs according to surfaces , it was 76.37 % on the lower surface , while it was 55.55 , 37.91 and 24.34% on the middle ,foot and terminal of leaflet .Leaflet dried and oval pustules which contain the spores of serenomyces phoenicis were recorded as symptoms represented the scorch disease while the other fungi caused yellowish and spotting symptoms sixteen species belonging to 13 genera were isolated from the leaflets , these species were as follows : Chaetomium atrobrunneum , C.globosum , Chalaropsis thielavioides , C.radicicola ,Stemphylium.sp.,Bipolaris australis ,Cladosporium herbarum , Nigrospora sphaerica , Diplodia phoenicis , Phoma glomerata , Fusarium verticillioides , Alternaria alternate ,bA. longipes ,Coniochaete sacchareo , Melanospora sp., andS.phoenicis.Most of of them caused blight disease .Among of them D. phoenicis and S. phoenicis gave scorch symptoms pathogenicity testing .The study epplained that Extract agare was the best medium for A. phoenicis growth ;it was 7 cm , while PCA gave the lowest growth, the fungus growth , the best growth 6.83 cm was noticed on 25 c while the growth stopped on 35 c . PH also effected on the fungus radial growth , it was 4.35 cm at PH4 , while it was 6.57 cm at PH 8 .Tested fungicides revealed significant differences . Dazim and Revus Top inhibited the radial growth in a percent of 100% ,while it reduced to 83.34% with the fungicides Hunter , so Dithane M4S inhibited the radial growth to 54.22% . The biological agent Trichoderma harzianum antagonists with D. phoenicis in the alevel 1,P. fluorescence also inhibited D. phoenicis in a percent OF 100%.The field experiment revealed that pa fluorescence reduced infection intensity to 50% , while Dazim biological agent T. harzianum treatment were the lowest treatment in reducing the infection intensity which reduced to 4.1 and 6.3 respectively .

استجابة زهرة الشمس للحامض الاميني البولين تحت مدد ري مختلفة == The Response of Sunflower (Helianthus annuus.L) to Proline under different Irrigation intervals

اسم المؤلف: شاكر اسماعيل عبد الرضا
اسم المشرف: هاشم رشيد مجيد
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - المحاصيل الحقلية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Field experiment was conducted during the fall season of year 2014 done in farmer's fields in the Auffia area southwest of Missan province (about 14 km .1 - m the soil was clay loam with 4.2 ds . city center)fromThe objectives were to determent the response of Sunflower (Helianthus annuus .L) hybrid Flame to amino acid - Proline in order to reduce water stress. The experiment executed in split - plot (R.C.B.D) design with three replicates. The experiment were three levels of proline (P1 spray Proline concentration in addition to the control 1 - ; P2 spray Proline concentration 100 mg L 1 - 50 mg LP0 as distilled water were located in sub - plot) while the other factor was irrigation period (W1= irrigation every 10 days; W2= irrigation every 15 days andW3= irrigation every 20 days) as the main - plot.The crop sprayed with Proline acid after mixed with water in two phases of growth is V6 (the phase of six real leaves) and R1 (flowering stage) with application of irrigation system. After the plants reaches to physiologic maturity and growth characteristics measured; the contains of yield and quality characteristics and the results were analyses statistically and compared with averages LSD test. The results showed the following.e uhigher valgave to the control compared 1 - 100 mg Lwith praying ProlineS - 1for growth measurement in mean of plant height; number of leaves; stalk diameter ; head diameter and LAI and they were (126. 19 cm ; 28. 60 leave ; 23. 27 mm; 17. 48 cm and 2. 28) respectively. Also increased the mean of yield and seed yield; weight of 100 seed the; 1 - head er of seedsnumb components) 1 - 16 ton h .and 1 1 - 48 tons h .0 4 g; 3 .40 seeds; 6 .(1003 oil yield ofrespectively.2 - Applying irrigation every 10 days improve all growth characteristics; yield's components and quality compared to applying irrigation every 20 days, which gave the lowest mean in above characters.3 - The results also showed that the interaction of applying Proline and irrigation periods was significant for the most of the growth characteristics; yield and quality characteristics. It was concluded from to reduced period of irrigation not differ from hich W .1 - 100 mg L Proline once applying daysevery 15 applying irrigation every 10 days for most of the studied characteristics. Is the most important yield; oil production and this can saves 25% of irrigation water under conditions - Missan Auffia area. That reduces the wastae of water resources or expansion of the agricultural area and increase the yield.

اســتـخـلاص السـكريات المـتــعددة مـــــن الطــحــلــب الاخضر Cladophora sp. وتوصيفها واســــتعمالها في اقراص اللحم البقري == Extraction and Characterization of Green Algae Cladophora sp. Polysaccharide and use it in Beef Patties

اسم المؤلف: سـهام ولـيـد عـلك الامارة
اسم المشرف: ام البشر حميد جابر الموسوي | روضة محمود العلي
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: الاغذية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: تضمنت الدراسة استخلاص السكريات المتعددة من الطحالب، اذ تــــــــــــــم جــــــــــــمع عـــــــــــــينات مـــــــــــــن الطحالب الخضراء من مياه شط العرب ضمن منطقة كرمة علي في البصرة وبعد التنظيف والتنقية والتجفيف تم تشخيصهواتبين انها تعود الى جنسCladophora sp. ، درس تركيبها الكيميائي على اساس الوزن الجاف من الرطوبة والرماد والبروتين والدهن والكاربوهيدرات اذ بلغت نسبة الرطوبة 4.16% والرماد 29.78% والبروتين 16.10% والدهن %1.25 والكاربوهيدرات %48.71. وتم استخلاص السكريات المتعددة من الطحالب بواسطة كاربونات الصوديوم Na2CO3، وقدر محتواها من السكريات الكلية اذ بلغت 78.4%. كذلك درس تاثير ظروف الاستخلاص على حاصل السكريات المتعددة شملت نسبة خلط المادة الاولية : المذيب (غم/ مل) اذ استعملت اربع نسب 7 : 1و9 : 1 و1 : 12 و15 : 1 حيث اعطت نسبة 9 : 1 اعلى حاصل واقل نسبة حاصل كانت %2.3بنسبة خلط 15 : 1. استعملت درجات حرارة (60 و80 و100) م حيث اعطت درجة حرارة 80 م اعلى نسبة حاصل %7.53، اما اقل نسبة حاصل فكان عند درجـــــــــــــــــة حرارة 100 م %2.09. درس تاثير مدة الاستخلاص واستعملت مدد زمنية (2 و4 و6) ساعة اذ اعطت مدة 2و4 ساعة نسبة حاصل 6.95 و%7.60، بينما اعطت مدة استخلاص 6 ساعات نسبة حاصل%3.40 وهي الاقل، ودرس تاثير الرقم الهيدروجيني حيث اعطى الرقــــــــم الـــــــــــــهيدروجيني 2 اعلى حاصل وهو 7.49مقارنة مع 4 و6 حيث كانت نسبتي الحاصل 2.33 و1.92% على التوالي. من خلال دراسة الظروف اعلاه تبين النتائج ان افضل نسبة حاصل تم الحصول عليها عند الاستخلاص بكاربونات الصوديوم بنسبة خلط 9 : 1 وبدرجة حرارة 80 م لمدة 4 ساعات وعند رقم هيدروجيني2. درست الخصائص الفيزيوكيميائية للسكريات المتعددة المستخلصة من الطحالب وكانت النتائج كالاتي : - 1 - اظهرت نتائج اللزوجة النسبية للسكريات المتعددة المستخلصة من الطحالب ان اللزوجة تزداد بزيادة التركيز وتقل بزيادة درجة الحرارة حيث كانت اعلى قيمة للزوجة بدرجة حرارة 30 م (11.0020) واقل قيمة للزوجة النسبية (8.0576) عند درجة حرارة 50 م وكانت لزوجة السكريات المتعددة المستخلصة اقل من لزوجة الجينات الصوديوم القياسية.2 - لوحظ ان قابلية السكريات المتعددة المستخلصة لامتصاص الماء وربط الدهن تزداد بزيادة التركيز وكانت مقاربة لقابلية الجينات الصوديوم القياسية. - كانت النسبة المئوية لذوبان السكريات المتعددة المستخلصة 69.72% وهي اعلى من ذوبان الجينات الصوديوم القياسية والبالغة 65.68%. 4 - اظهرت النتائج انخفاض قابلية السكريات المتعددة على تكوين الرغوة.5 - قدر الوزن الجزيئي للسكريات المتعددة المستخلصة بقياس لزوجة السكريات المتعددة المستخلصة من الطحالب اذ تم تقدير اللزوجة الحقيقية لاستخراج الوزن الجزيئي الذي بلغ 875.26 كيلو دالتون. تم دراسة التحلل الوزني الحراري للسكريات المتعددة المستخلصة وكان الفقدان بحدود7% والمتبقي93% عند درجة حرارة 515.27 م.6 - اختبرت السمية الخلوية للسكريات المتعددة المستخلصة من الطحالب اتجاه كريات الدم الحمر للانسان واظهرت النتائج عدم سميتها لكافة التراكيز المستعملة ولمدد الحضن 10 و30 و60 دقيقة.7 - تم ادخال السكريات المتعددة المستخلصة والجينات الصوديوم القياسية في اقراص اللحم البقري وبنسب 0.2 و0.4 و0.6% وخزن بالتبريد بدرجة حرارة 4 م ولمدد زمنية1 و4 و7 يوم.8 - درست الصفات الفيزيائية لاقراص اللحم البقري اظهرت النتائج ارتفاعا معنويا p<0.05)) في قـــــــيم قابلية حمل الماء لاقراص اللــحم المعاملة بالــــــــــــــسكريات الـــــــــــــــــمتعددة (المعاملة المحضرة) والجينات الصوديوم (المعاملة القياسية) مقارنة بالعينة الضابطة. بينت النتائج ان الرقم الهيدروجيني في اقراص اللحم يزداد معنويا بتقدم مدة الحفظ بالتبريد ولوحظ انخفاض نسبة الفقد في الوزن اثناء الطبخ وارتفاع نسبة حاصل الطبخ في اقراص اللحم البقري المعاملة بالسكريات المتعددة والجينات الصوديوم القياسية مقارنة بالعينة الضابطة. 9 - درست الصفات الحسية لاقراص اللحم والتي شملت اللون والنكهة والطراوة والعصيرية والقبول العام وقد اظهرت بعضها اختلافا معنويا((p<0.05 خلال مدة الحفظ بالتبريد ولم يظهر البعض الاخر اختلافا معنويا فالنسبة لدرجات تقييم اللون لاقراص اللحم المفروم لم تتاثر معنويا عند معاملة اقراص اللحم بالسكريات المتعددة المحضرة والجينات الصوديوم القياسية، اما درجات تـقييم صفـــــــــــــــــة النـــــــــــكهة لاقراص اللحــــــــــم فقد تاثرت معنويا p<0.05)) عند المعاملة بالسكريات المتعددة المحضرة والجينات الصوديوم القياسية مقارنة بالعينة الضابطة. | The study included extraction of polysaccharides from algae. The green algae was collected from Shatt Alarab water in Karmat Ali in Basrah, the green algae was purified and it is Cladophora sp. Polysaccharides was extracted by sodium carbonate Na2CO3. It̓s chemical composition including moisture, ash, protein, fat , carbohydrate and total saccharides was studied and it was moisture 4.16%, ash 29.78%, protein 16.10% at 1.25%, carbohydrate 48.71% and total sharrides 78.4%. The effect of extract conditions on polysaccharides yield were also studied included mixing percentage 1 : 7, 1 : 9, 1 : 12 and 1 : 15 and 1 : 9 exhibited the highest yield (7.48%) and the lowest yield (3.2%) when we use 1 : 15. The extraction was carried on different temperature 60°C, 80°C and100°C the highest yield was 7.53% when extraction on 80°C .The effect of the time of extraction in 2 hrs, 4hrs so the yield was 6.95% and 7.60% for 2 and 4 hrs respectively, the highest yield was 7.49% when extraction on pH 2. The results showed that the best yield was when the extraction on 1 : 9 and 80 °C for 4hrs and pH 2. The physiochemical properties for polysaccharides wer studied and the result showed the relative viscosity was increased with the increase of concentration and decrease with the increase of temperature the highest viscosity was 11.0020 in 30°C and the lowest was 8.0576 in 50 °C. The ability of polysaccharides for water absorbtion and fat binding was noticed that it was increased with the increased of concentration, the percentage of polysaccharides solubility was 69.72%, and it was higher than sodium alginate (65.68%). The results also showed decrease of foaming of polysaccharides because of it̕s high viscosity. The molecular weight of polysaccharides was 875.26 Kdal, the Thermo Gravimetric Analysis (TGA) was 7% for loss and the residue was 93%. The test of cytotoxicity was appaired that the polysaccharides was no toxic in all concentration and for all incubation periods 10, 30 and 60 min. The extracted polysaccharides and sodium alginate were used in beef patties for 0.2%, 0.4% and 0.6% and storage for 1, 4 and 7 days, the results showed that there were significant increased (p<0.05) in water holding capacity in all treatments comparing with control and pH increased during storage periode and also noticed that cooking loss was decreased and increased in cooking yield for beef patties treated with polysaccharides and sodium alginate comparing with control. The results showed that addition of extracted polysaccharides and sodium alginate to beef patties improved sensory properties (color, falvor, tenderness, juicness and overal acceptability) during storage periode compared with control.

تاثير مستويات مختلفة من السماد الفوسفاتي في نمو وحاصل ثلاثة اصناف من محصول الحنطة Triticum aestivum L == Effect of different levels of Phosphate fertilizaer on growth and yield of three cultivars of Wheat (Triticum aestivum L.)

اسم المؤلف: سندس كامل جبار الحلفي
اسم المشرف: هاشم رشيد مجيد
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - المحاصيل الحقلية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: نفذت تجربة حقلية خلال الموسم الشتوي 2013 / 2014 في حقول احد المزارعين في منطقة الشنانة التابعة لقضاء القرنة ( 65 كم شمال مركز محافظة البصرة ) في تربة مزيجية طينية بهدف دراسة استجابة ثلاثة اصناف من الحنطة الناعمة . Triticum aestivum L (اللطيفية وابوغريب - 3 وتموز - 2) في النمو والحاصل والنوعية لتاثير اضافة اربعة مستويات من السماد الفوسفاتي ( 0 و40 و80 و120 كغم P2O5 ه - 1 ) طبقت التجربة باسلوب التجارب العاملية ووزعت المعاملات وفقا لتصميم القطاعات العشوائية الكاملة بثلاثة مكررات . اظهرت نتائج الدراسة ان الاصناف اختلفت معنويا فيما بينها في معظم الصفات المدروسة اذ تفوق الصنف اللطيفية في صفة ارتفاع النبات ( 96,44 سم) ومساحة ورقة العلم (32,97 ســم2) وطول السنبلة (10,41سم) وعدد الحبوب في السنبلة (61,50 حبة سنبلة - ١) السنبلة ونسبة البروتين (11,84 ٪) في حين تفوق الصنف ابوغريب - 3 في صفة عدد الاشطاء(600,35 شطا م - 1) وعدد السنابل (572,07 سنبلة م - 1) ووزن 1000 حبة ( 31,09 غم ) وحاصــل الحبوب ( 6,31طن هــ - ١ ) والحاصل الحيوي( 14,64 طن هــ - ١ ). واشارت النتائج الى تفوق المستوى السمادي 120كغم P2O5 هـ - ١ معنويا في صفات ارتفاع النبات (99,11ســم ) ومساحة ورقة العلم ( 35,74 ســم2 ) وطول السنبلة (10,68 ســم) وعـــدد الاشطاء الكلي(621,82 شطا م - 1) وفي جميع صفات الحاصل وحاصل الحبوب( 6,68 طن هــ - ١ ) والحاصل الحيوي (14,98 طن ه - 1) ودليل الحصاد(44,58 ٪) ونسبة البروتين في الحبوب (12,62 ٪) . كان التداخل معنويا بين الاصناف ومستويات الفسفور في حاصل الحبوب وحققت التوليفة للصنف ابو غريب - 3 اعلى متوسط لحاصل الحبوب (6,98 طن هــ - ١) ويلاحظ من النتائج ان زيادة حاصل ابو غريب - 3 عند المستوى 120 كغم P2O5 هـ - ١ هو نتيجة لزيادة عدد الاشطاء (669,33 شطا م - 1 ) وزيادة مكونات الحاصل وهي عدد السنابل(636,81 سنبلة م - 1) ووزن 1000 حبة(33,03 غم) . | A field experiment was conducted during winter season of 2013 - 2014 at AL - shinana that belong to AL - Qurna about ( 65) Km north Basrha in silty loam soil in order to determine the best production of the three cultivars of wheat (Triticum aestivum L.) (Abu Graib - 3 , Tammoz - 2 and Latifia ) to determine the best level of phosphate fertilizer (0 , 40 , 80 , 120 kg P2O5 hˉ1 ) .The experiment design was R C B D with treatments arranged in factorial in three replicates . Results showed that cultivars are different in it's characteristics as the Latifia superior in plant height (96.44 cm ) flag leaf area ( 32.97 cm2 ), spike length ( 10.41 cm ) number of grain per spike( 61.50 ) and protein content ( 11.84% ). Abu Graib - 3 superior in number of tillers .mˉ2(600.35) number of spikes .mˉ2(572.07) weight of 1000 grain ( 31.09 gm) , grain yield (6.31 ton.h - 1 ) biological yield (14.64 ton.h - 1 ) Results showed a variance among fertilizer level where 120 kg P2O5 hˉ1 caused highest number of , plant height (99.11 cm ) flag leaf area (35.74 cm2) , spike length ( 10.68 cm), number of tillers (621.82 ) and all yield component and grain yield ( 6.68 ton .hˉ ¹), biological yield ( 14.58 ton .hˉ ¹) , protein (12.628% ) and harvesting index(44,58 %) . Results also showed a significant interaction between cultivars and P levels, grain yield which increased in all cultivars with increase in P levels grain yield obtained at treatment Abu - Graib - 3 ×120 kg P2O5 hˉ1 (6.98 ton.h - 1) the increase in grain yield Abu - Graib due to higher yield components number of spike m - 2 (636. 81) and 1000 - grain weight (33.03 gm

معالجة مياه الصرف الصناعي لمعمل الاسمدة في المنطقة الجنوبية واعادة استعماها لري نبات الطماطة Lycopersicon esculentum Mill == Industrial wastewater treatment of fertilizer factory south region and reuse for tomato irrigation( Lycopersicon esculentum Mill.)

اسم المؤلف: سماح رسول جويد العكيلي
اسم المشرف: محمد عبد الله عبد الكريم | نجلة جبر محمد الاميري
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: التربة والمياه
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The study was conducted to investigate the possibility of using the effluent originated from fertilizers factory/ south region, Basrah province for tomato irrigation. Effluent samples were collected from urea unite line, ammonia unite lion, and collection basin at nine periods during 3\9\2015 to 5\8\2016. Tap water was used as control water. Three types of filters were used to enhance the characteristics of water collected from the fertilizers factory which are Rice Husk Ash filter, sand filter and Rice Husk Ash + sand filter(75 : 25). chemical characteristics (EC, PH, Ca++, Mg++, Na+, K+, Cl - , SO4 - - , total hardness, NO3 - , NH4+, urea) were obtained before and after filtration then Removal efficiency (%) was calculated. Basin on above experiment, a pot culture experiment was conducted to study the effect of fertilizers industry effluents on chemical parameters of soil and its impact on growth parameters of the tomato plant. Each pot was prepared by filling loamy sand soil 10 kg collected from a farm nearby the factory. All the pots were fertilized by manure, phosphorus and potassium. Nitrogen fertilizer (urea) was applied an equivalent of 0, 50% and 100% of recommended level (300 kg N ha - 1). Tomato seedlings (hybride Newton F1) were transplanted in the pots. The pots were irrigated with above fertilizer industry effluents or tap water after the seedlings standing in the pots. After 90 days, soil and plant samples were collected to measure EC, pH, NO3 - in soil, NH4+ in soil, plant height, shoot dry weight, N concentration,number of flowers plant - 1 and number of fruits plant - 1.The results showed that there was a significant changes among sample periods in all water characteristics. The highest removal efficiency of urea, NH4+, NO3 - , cations and anions were recorded by using Rice Husk Ash filter, while the lowest efficiency were recorded by using sand filter. Data alsoBshowed that chemical parameters (EC, NH4+ and NO3 - ) of soil were differed by different filter used and follow the order : control (without filtration) < sand filter < Rice Husk Ash + sand filter < Rice Husk Ash filter. This result was reflected on plant growth parameters (plant high, shoot dry weight, N concentration ,number of flowers plant - 1 and number of fruits plant - 1) and follow the order : Rice Husk Ash + sand filter < Rice Husk Ash filter < sand filter < control. However soil pH was not affected at different filters used. Increasing the level of nitrogen significantly increased EC, NH4+ and NO3 - in soil and decreased soil PH resulted in increased plant parameters. Soil chemical parameters and plant growth parameters were differed by different source of fertilizers industry effluents and follow : ammonia line < urea line < collection basin < tap water. Data also revealed that tomato plant irrigated with water of ammonia line and filtered through Rice Husk Ash + sand filter showed best growth parameters as compared with other plants with higher shoot dry weight of 11.69 g plant - 1. The pot experiment suggested the possibility to using the water of ammonia line which filtered through Rice Husk Ash + sand filter with 50% of nitrogen recommended level to enhance tomato growth and it would save costs on fertilizer and reduction in pollution load of soil and water.

المكافحة الاحيائية لمرض الذبول الفيوزارمي في الطماطا المتسبب عن الفطر Fusarium oxysporum f.sp.lycopersici باستخدام الفطر Trichoderma harzianum وفطر المايكورايزا Glomus mosseae == Biological control of tomato Fusarium wilt caused by Fusarium oxysporum fsp.lycopersici by using Trichoderma harzianum and mycorrhiza Glomus mosseae

اسم المؤلف: سلام نجم عبود الاسدي
اسم المشرف: يحيى عاشور صالح
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - الوقاية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في مختبرات قسم وقاية النبات /كلية الزراعة / جامعة البصرة خلال الفترة من 1/11/ 2015 الى 1/6/2016 لتقييم كفاءة فطر المايكورايزا G. mosseae والعامل الاحيائي T.harzianum في مكافحة مرض الذبول الفيوزارمي لنبات الطماطا المتسبب عن الفطر Fusarium oxysporum fsp.lycopersici . اظهرت النتائج دور العامل الاحيائي في تثبيط نمو الفطر الممرض بطريقة الزراعة المزدوجة حيث كان التضاد من الدرجة 1 حسب مقياس Bell . كما اظهر راشح الفطر الاحيائي T.harzianum تفوقوااضحا في تثبيط نمو الفطر الممرض . اوضحت نتائج تاثير فطر المايكورايزا G.mosseae والعامل الاحيائي T.harzianum والتداخل بينهما في انبات البذور ونمو نبات الطماطا ( تجربة الاصص) وجود فرق معنوي في النسبة المئوية لا نبات البذور ، اما في ما يخص اطوال النبات بعد ستة اسابيع من الانبات فقد اشارت النتائج الى وجود فروق معنوية بين المعاملات حيث بلغت اطوال النباتات 4.55 و18.22 و14.44 و18.00 و15.33 و15.44 و15.55 سم على التوالي للمعاملات Fusarium oxysporum f.sp.lycopersici(f) وTrichoderma harzianum(T.h) وGlomus mosseae(G.m) ومعاملة التداخل بين العامل الاحيائي وفطر المايكورايزا GT ومعاملة التداخل بين فطر الممرض وفطر المايكورايزا GF ومعاملة التداخل بين العامل الاحيائي وفطر الممرض TF ومعاملة التداخل بين العامل الاحيائي وفطر المايكورايزواالفطر الممرضر GTFقياسا بمعاملة المقارنة التي بلغت 12.33 سم ، وكان اكثر الفطريات تاثيرا في زيادة اطوال النباتات العامل الاحيائي T.harzianum كما حقق التداخل بين العامل الاحيائي T.harzianum وفطر المايكورايزا G.mosseae زيادة معنوية في الوزن الطري للمجموعين الخضري والجذري حيث بلغ الوزن الطري للمجموعين الخضري والجذري 6.27 و0.72 غم على التوالي قياسا بمعاملة المقارنة التي بلغ الوزن الطري للمجموعين الخضري والجذري فيها 2.83 و0.51 غم على التوالي . اما التجربة الحقلية فقد اظهرت نتائجهواجود فروق معنوية في شدة الاصابة بالفطر الممرض حيث كانت اقل شدة اصابة في المعاملة التداخل بين فطر المايكورايزواالفطر الممرض GF اذ بلغت 22.21% تلتها المعاملات TF وGTF حيث بلغت شدة الاصابة فيها 27.77 و35.01% على التوالي قياسا بمعاملة المقارنة البالغة 48.02% ، اما اطوال النبات فقد ازداد بشكل معنوي في المعاملة GTF اذ بلغت 72.1 سم والتي تفوقت بفروقات معنوية عالية تلتها المعاملات GT وG وGF وT اذ بلغت 69.9 و68.9 و65.4 و63.8 سم على التوالي اذ اختلفت معنويا عن معاملة المقارنة التي بلغت 55.3 سم ومعاملة الفطر الممرض البالغة 47.2 سم ، وكان اعلى وزن طري للمجموع الخضري في المعاملة TF اذ بلغت 40.0 غم تلتها المعاملات GF وG وGTF وGT حيث بلغ الوزن الطري لها 35.0 و34.5 و32.3 و30.0 و29.7 غم / نبات والتي اختلفت معنويا عن معاملتي المقارنة والفطر الممرض اذ بلغت 17.33 و13.7 غم . وان اعلى زيادة في الوزن الطري للمجموع الجذري بلغت 29.53غم لمعاملة المايكورايزا G.mosseae والتي اختلفت معنويا عن معاملة المقارنة البالغة 17.7 غم . اشارت النتائج الى دور فطر المايكورايزا G.mosseae في زيادة وزن حاصل الثمار اذ بلغ الوزن 3.067 كغم / نبات قياسا بمعاملة المقارنة التي بلغت 1.630 كغم / نبات وبفارق معنوي ، وبينت النتائج دور فطر المايكورايزواالعامل الاحيائي في زيادة الفعالية الانزيمية لا نزيم البيروكسديز فقد اعطت معاملة فطر المايكورايزا G.mosseae اعلى نسبة اذ بلغت 1.710 وحدة / غم وزن رطب وقد اختلفت معنويا عن معاملة المقارنة البالغة 0.510 وحدة / غم وزن رطب .اوضحت نتائج التجربة الحقلية ايضا كفاءة العامل الاحيائي وفطر المايكورايزا في زيادة كمية الفسفور في نباتات الطماطا حيث بلغت اعلى نسبة للفسفور في معاملة التداخل GF حيث بلغت 38.93 ملغم / كغم تلتها المعاملات G وT وGT وGTF وTF وF اذ بلغت نسبة الفسفور فيها 38.44 و28.93 و28.93 و26.58 و23.91 و13.25 ملغم / كغم على التوالي قياسا بمعاملة المقارنة اذ بلغت 21.23 ملغم / كغم. وبينت النتائج دور فطر المايكورايزا G.mosseae والعامل الاحيائي T.harzianum في زيادة نسبة وشدة اصابة الجذور حيث بلغت اعلى نسبة وشدة اصابة لمعاملة التداخل GT 66 و58% على التوالي وقد تفوقت بفروقات معنوية عالية تلتها المعاملات G وGTF وGF حيث بلغت نسبة وشدة الاصابة فيها 38.00 و36.66% و37.66 و34 % و31.33 و32.00% على التوالي . وبينت النتائج ايضواجود فروقات معنوية في عدد ابواغ فطر المايكورايزا G.mosseae حيث بلغ اعلى عدد للابواغ 45.83 بوغ / نبات في معاملة التداخل GT تلتها المعاملات GF وG وGTF حيث بلغ عدد الابواغ فيها 29.16 و22.50 و16.66 بوغ / نبات . | This study was conducted in the laboratories of the Department of Plant Protection , College of Agriculture , University of Basra .during the period from 1/11/2015 - 1/6/2016 ,to evaluate the interaction efficiency between Glomus.mosseae and bio agent Trichoderma.harzianum against tomato fusarium wilt disease caused by Fusarium oxysporum f.sp lycopersici . .The results showed the role of T.harzianum in inhibiting the growth of pathogenic fungus where the antagonism degree is of class 1 according to Bell scale. Also it showed that T.harzianum exaudate inhibited the growth of pathogen. The results also explained a significant effect of mycorrhiza G.mosseae and T.harzianum and their interaction on seed germination percent and growth of tomato plant (pots experience) , As regards to the lengths of the plants after six weeks from germination the results indicated a significant difference among the treatments , the lengths of plants reached 4.55 ,18.22 , 14.44, 18.00, 15.33, 15.44, 15.55, cm for the treatments , F, T ,M, MT , MF , TF and MTF, respectively compared to control which amounted to 12.33 cm ,T.harzianum led to increase the lengths of Plant significantly .The interaction between T harzianum and G.mosseae (TM ) also led to increase the fresh weight in comparison with control which reached respectively 2.83 and 0.51. The field experiment results showed significant differences in the severity of the infection with pathogen where the less severe of the infection was found in the treatment MF which was 22.21 % followed by TF and MTF treatments which were 27.77 and 35.01% respectively. The plant lengths also significantly increased , it reached 72.1 cm in the treatment MTF followed by the treatment MT , M , MF and T which reached 69.9, 68.9 , 65.4 and 63.8 respectively , which they significantly differed with control and pathogen treatments which were 55.3 and 47.2 cm respectively . The treatment TF led to increase the shoot fresh weight up to 40.0 gm. , followed by MF , M,T, MTF and MT which were 35.0, 34.5 , 32.3 , 30.0 and 29.7 which significantly differed with control and pathogen treatment which reached 17.33 and 13.7 respectively . The root fresh weight also increased when G.mosseae was used , it reached 29.53 gm. compared with control (17.7 gm.).The results indicated the role of G.mosseae in increasing fruit weight which reached 3.067 kg Plant compared with control which was 1.630 kg/plant .The results also showed the role of G.mosseae in increasing the enzymatic activity of peroxidase which reached 1.710 unit/gm. Compared to control which reached 0.510 unit/ gm wet weight . The results of field experiment also revealed that T.harzianum and G.mossese increased the amount of phosphor in tomato Plants in a percent of 38.93 , 38.44 , 28.93 , 28.93 , 26.58 , and 23.91 mg / kg for the treatment MF , M , T , MT , MTF and TF respectively in with comparison with control which was 21.23 mg / kg. From other hand , the interaction between G.mosseae and T.harzianum led to increase the root infection percent and infection up to 66 and 58% respectively the treatment M, MTF , MF as they were 38.00 , 36.66% 37.66 , 34% 31.33 , 32.00% . Finaly the results elucidate that the interaction between G.mosseae and T.harzianum led to increase the number of spores of G.mosseae reaching to 45.83 spore/ plant followed by the treatment M , MTF , MF , which gave 29.16 , 22.50 , and 16.66 , spores / plant respectively

الكشف عن بكتريا E.coli O157 : H7 في لحوم الابقار الطازجة المفرومة ودراسة تاثير بعض مواد الحفظ الحيوية على عيوشيتها بظروف الخزن بالتبريد == Detection of E.coli O157 : H7 in ground beef meat and study the antimicrobial effect of some bio Protection materials on bacterial survival during cooling storage

اسم المؤلف: سحر صبيح جورج
اسم المشرف: نوفل عبد الامير حسين الحلفي | صباح مالك حبيب الشطي
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الاغذية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: اجري الكشف عن تواجد بكتريا E. coli O157 : H7 في عينات لحم الابقار الطازجة المفرومة لخمس مناطق مختلفة في محافظة البصرة شملت (الجزائر وابي الخصيب والزبير والبصرة القديمة وكرمة علي) خلال الفصول الاربعة على مدار اشهر سنة 2013، وقد وجد ان هناك تفاوتا كبيرا في تواجد هذه البكتريا المرضية المصلية، اذ بلغ اعلى مستوى تلوث للحم الابقار الطازجة المفرومة لمنطقة البصرة القديمة تليها مناطق الزبير وكرمة علي والجزائر بينما لم يلاحظ اي تلوث لعينات لحم الابقار الطازجة المفرومة لمنطقة ابي الخصيب، وقد لوحظ تواجد بكتريا E. coli O157 : H7 خلال اشهر (شباط، اذار، نيسان، تموز، تشرين الاول وتشرين الثاني) التي امتازت باعتدال درجات الحرارة والرطوبة النسبية .تم الحصول على 27 عزلة موجبة لبكتريا E.coli O157 : H7 من اصل 540 عينة لحم بقر طازج مفروم وبنسبة 5% شهريا على مدار سنة كاملة وشخصت اعتمادا على استخدام الاوساط الانتقائية الخاصة Sorbitol MacConkey Agar المدعم بالسفكسيم تولريت واجراء الفحوصات المظهرية والمجهرية والكيموحيوية باستخدام نظام API20E واجراء فحص التلازن باستخدام اشرطة اللاتكس للتشخيص الدقيق، كما استخدمت التقنية الحديثة وهي شرائح البتري فلم petrifilm لعد بكتريا القولون الكلية وE. coli شهريا على مدار السنة، اذ بلغت اعلى متوسطات لوغاريتم اعداد بكتريا القولون الكلية (6.15 ، 6.22 و6.43)cfu/g وللاشهر (ايلول، تشرين الاول وتشرين الثاني)على التوالي لمنطقة البصرة القديمة والتي كانت الاكثر تلوثا مقارنة مع بقية المناطق المشمولة بالدراسة وكذلك الحال بالنسبة لبكتريا E. coli بينما سجلت منطقة ابي الخصيب اقل مستويات لتلوث اللحوم الطازجة المفرومة اذ بلغ المتوسط اللوغارتمي لاعداد بكتريا القولون الكلية وE. coli ( 1.52 و1.53) cfu/g خلال شهري (شباط وايار) على التوالي.استعمل الكيتوسان المحضر بطرق الاستخلاص الكيميائية التقليدية من قشور الروبيان والحوامض العضوية(الخليك واللاكتيك) ونواتج ايض بكتريا حامض اللاكتيك كمواد حافظة طبيعية ضد النشاط البكتيري لاطالة العمر الخزني للحوم المفرومة والبيركر المصنع من اللحم البقري المفروم ومستخلص ماء اللحم المعقم وغير المعقم لمدة 12 يوم من الخزن بالتبريد بالثلاجة عند درجة حرارة 4 م وباستخدام ثلاث تراكيز مختلفة .اظهرت نتائج الدراسة تاثير اضافة الكيتوسان بانواعه (Kp الكيتوسا القياسي وK3 الكيتوسان المؤستل بالاوتوكليف لمدة ساعة وK2 الكيتوسان المؤستل بالاوتوكليف لمدة نصف ساعة وk1 الكيتوسان المؤستل بالظروف الجوية لمدة ساعة) وبتراكيز (0.75 و1 و1.5 )% خلال الخزن بالتبريد على متوسطات لوغارتيم اعداد بكتريا (E. coli O157 : H7 وcoliform bacteria وE. coli وTotal count bacteria ) اذ لوحظ اعلى تثبيط عند تركيز 1.5% لمعاملة Kp لجميع انواع البكتريا اعلاه تواليا لعينات لحم البقر الطازج المفروم والبيركر ومستخلص ماء اللحم المعقم وغير المعقم والتداخل بينهم وقد بينت نتائج التحليل الاحصائي وجود فروق معنوية (p<0.05) لتاثير التركيز ومدة الخزن بالتبريد ونوع البكتريا للمعاملات اعلاه، وقد وجد هناك انخفاض معنوي (p<0.05) للوغاريتم اعداد بكتريا E.colio157 : H7) وcoliform bacteria وE. coli وTotal count bacteria ) عند درجة حرارة 4 م لمعاملات الحوامض العضوية (الخليك، اللاكتيك والخليط الحامضي ) بتقدم مدة الخزن بالتبريد مقارنة مع السيطرة الموجبة وللتراكيز (0.75 و1و1.5)% وقد حددت المعاملة الافضل في التثبيط البكتيري وهي حامض الخليك اذ بلغت ادنى متوسطات لاعداد البكتريا عند تركيز 1.5% في نهاية مدة الخزن بالتبريد، كما لوحظ انخفاض معنوي (p<0.05) لمتوسطات لوغارتيم اعداد بكتريا E. coli O157 : H7) وcoliform bacteria وE. coli وTotal count bacteria ) عند درجة حرارة 4 م اذ بدات بالانخفاض التدريجي بتقدم مدة الخزن بالتبريد مقارنة مع معاملات السيطرة للتراكيز (0.75 و1 و1.5) %اذ بلغ ادنى متوسط لوغارتمي لاعداد البكتريا اعلاه عند تركيز 1.5 % عند نهاية مدة الخزن بالتبريد .وقد اشارت نتائج التحليل الاحصائي الى وجود فروق معنوية (p<0.05) لكل من التركيز ونوع المعاملة ومدة الخزن بالتبريد، وقد بينت نتائج التحليل الاحصائي وجود فروق معنوية (p<0.05) لتاثير اضافة نواتج ايض بكتريا حامض اللاكتيك (L.p, L.c وL.p+L.c)على متوسطات لوغاريتم اعداد بكتريا E. coli O157 : H7)و coliform bacteria وE. coli وTotal count bacteria) اذ اظهرت معاملة LP افضل نتائـــج تثبيط بالمقارنة مع معاملتي ( LCو LP+ LC )عند تركير 1.5 % وعند نهاية مدة الخزن بالتبريد ولجميع انواع البكتريا اعلاه على التوالي. | The presence of E. coli O157 : H7 bacteria I fresh ground beef samples about five different areas in Basra included (Al - jazaaer - Abu alkhaseab - Zubair - Basra al kadema and Karmat Ali) durig the foure seasons over all months In (2013), a large contamination was found in the presence of these pathogenic bacteria serological E. coli O157 : H7 ,the highes serologically of fresh ground beef from Basra al kadema then ALzubair, Karmat Ali and Aljazaaer, while no any contamination notice fresh ground beef samples from Abu alkhaseab. The presence of E.colio157 : H7 bacteria during (February - March - April - Juli - October and Novenber) in this year. Which was characterized by moderate temperatures.27 E.colio157 : H7 straing were isolated from 540 samples of fresh ground beef by 5% per at all months in the year.Then characterize by using the selective culture media Sorbitol MacCokey Agar (SMA) compatible with Cefixime tellurite and phenotypic test and microscopic - biochemical test using API20E system and latex agglutination from exact diagnosis .Technology which is petrifilm to enrcumation coliform and E.coli bacteria through this study. The highest logarithm number of preparation of total coliform bacteria (6.15, 6.22 and 6.43) cfu/g in (September - October and Novenber) from Basra al kadema, which was the most contaminated compared with other areas included in this study, as well as from E.coli bacteria while Abu al khaseab area recorded the lowest levels of coliform and E.coli bacteria (1.52 and 1.53 ) cfu/g durig (February and May) respectively.Chitosan was used record by chemicals extraction methods, organic acid and free cell supernatant of metabolism of lactic acid as material natural preservative agents to that decrease the life storage time of fresh ground beef - burger - extract sterile and non serial water meat for 12 day by storage at 4C refrigerator and using three different concentrations.Studied the effect of adding the chitosan (KP, K3, K2 and K1) in (0.75, 1 and 1.5)% concentrations during the cooling storage ,the bacteria prepared are (E.colio157 : H7, coliform bacteria , E.coli and total count bacteria ) showed greater inhibitions at concentration 1.5% from KP treatment for all specie of bacteria are above.At these results of the statistical analysis show significant differences at (p<0.05) for effects of period of storage, type of bacteria among fresh ground beef - burger - extract serial and non serial water meat . There is no significant differences (p<0.05) in a (E.colio157 : H7, coliform bacteria, E.coli and total count bacteria ) at 4c then it declines gradually in progress from cooling storage compared with control coefficients of concentration (0.75, 1 and of 1.5)% as the lowest log number these bacteria at 1.5% concentration in the end of period cooling storage. This results indicated statically analysis and significant differences (p<0.05) for each type of bacteria, concentration, treatment and period cooling storage. The present studied lowest significant (p<0.05) for log number in (E.colio157 : H7, coliform bacteria , E. coli and total count bacteria ) at 4c organic acid (acidic acid, lactic acid and the mixed of there tow organic acid )in cooling period storage compares with positive control of (0.75, 1 and of 1.5)% concentration. The best treatment in these results was acidic acid then the lowest log number of bacteria recorded at 1.5% concentration in the end period cooling storage . statically analysis showed significant differences(p<0.05) for effect of adding the free cell supernatant of metabolism of lactic acid bacteria (LP, LC and LP+LC) then the best treatment in these results were LP that inhibitions compeers with (LC and LP+LC) at 1.5%concentration in the end of the cooling storage period for (E.coliO157 : H7, coliform bacteria, E. coli and total count bacteria )

دراسة بيئية مقارنة لتاثير الحاجز المائي في قضاء المدينة عند اسفل نهر الفرات == A comparative ecological study to the impact of Mudayna district barrier at the lower reaches of Euphrates River

اسم المؤلف: سجاد عبد الغني عبد الله
اسم المشرف: صادق علي حسين | عبد العزيز محمود عبد الله
الموضوع العام: علوم الزراعة
السنة: 2015
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: An Ecological study was conducted, on monthly basis, for the period from November 2013 to October 2014, on the Lower reaches of the Euphrates River covering a location at northwest of Basrah province within the area between Al - Chebaysh district of Thi Qar province, and Mudayna district of the city of Basrah. The study area covered a distance of 16 kms lies between latitudes 47˚00 ' 18.81 " and 47˚09 '55.46" E and latitudes 30˚57' 41.53" and 30˚57'7.38" N. On 2010 the Ministry of Water Resources constructed an obstruction on the Euphrates River, 11 km west of Mudayna of a width 760 m. It’s aims is to raise water levels in the western side of the river for water to flow to Hor Al - Chebaych. Three stations were selected to execute the study. The former was situated in front of the Chebaish district and with a distance of nine kilometers east where station 2 was located. However, to the east of the constructed embankment and just three kms apart station 3 was chosen. The study revealed that air temperature ranged from 11 - 45°C and water from 10.5 - 37°C, light penetration from 15 to 210 cm. Turbidity from 0.66 NTU in October at station3 and 40.8 NTU in September at station1. Current speed ranged between 0.01 m/s to 0.41 m/s.The lowest value of Electric conductivity (2.4μS/cm) recorded in November at station 3 and the highest (5.74μS/cm) in March at station 1. Total dissolved solids between 1788 mg/ L to 3832 mg/L. the lowest value of pH (7.4) measured in August at station 2 and the highest (8.7) in February at Station 3. Dissolved oxygen ranged from 5.5 to 10.1 mg/L. BOD5 ranged from 0.19 to 3 mg/L. The lowest value of salinity (1.3 g/L) recorded in June and July from Station 2 and the highest (2.9 g/L) was encountered in February at station 3. Total hardness the lowest (880 mg/L) encountered in July at station2 and the highest (1350 mg/L) in February at station3. Total alkalinity ranged from 84 to 182 mg/L. Reactive nitrate ranged from 0.6 to 37.7μg at. N/L. Reactive phosphate ranged from 0.023 to 1.857μg at. P/L. Concentrations of some heavy metals were also studied. Copper was impalpable in November and December in stations2 and 3, but the highest (1.90 μg/L) was in August at station2. Intangible value was also recorded for Lead in November at station3 and the highest (0.1 μg/L) in July of the same station. Non - tangible value encountered for cadmium in November and December at stations 2 and 3 but the highest (12.73 μg/L) in July for station1. The study took in consideration application of the general index to the quality of water (CCME) as seasonal readings were calculated depending on river maintenance system for the year 1967 and amended in 1980. Throughout the study period values ranged between marginal (56.3) and FairB(69). Values were obtained depending on the previous studies, as were between marginal (47) and Fair( 65). Fish community structure was described. A total of 11851 specimens were collected included 28 species and 25 genera belonging to 12 families all belong to class bony fishes Osteichthyes. Cyprinidae came first concerning the number of species (13) and genera (11) and composing 37.44 %, these are Hamri (Barbus luteus), Prussian carp (Carassius auratus), grass carp (Ctenopharyngodon idella), (Acanthobrama marmid), (Alburnus mossulensis), Shillug (Aspius vorax), bunni (Barbus sharpeyi), gattan (Barbus xanthopterus), Hemicultuer leucisculus), small mouth benine (Cyprinon kais), big mouth benine (Cyprinon macrostomum), (Garra rufa) the mullet family Mugilidae formed 35.50 % and consist two species , namely, Liza abu green and Liza subviridis. Tilapia Cichlid family was represented by 21.84 % and two species, namely Tilapia zilli and Oreochromis aureus. Gumbusia (Pociliidae) formed 2.70% and represented by two species , namely (Gambusia holbrooki) and Poecillia latipinna, Clupeids family accounted for 0.025% and represented by two species, namely Tenualosa ilsha) and Nematalsoa nasus. However one species of seven families included Siluridae (Silurus triostegus) 0.58%, Sparidae Acanthopagrus latus (arabicus) 0.57%, Mastacembelidae (Mastacembelus mastacembelus) 0.28% , Hemiramphidae Hyporhampus limbatus)) 0.23% and minnows Engraulidae (Thryssa whiteheadi) rate of 0.345 % and Gobiidae (Bathygobius fuscus) was collected for the first time in the study area and in station 3 at 0.050 % and Heteropneustidae (Heteropneustes foosilis) formed the lowest contribution. The study also was taken in consideration the diversity indices in the Euphrates. The lowest value (1.04) of Shannon diversity index (H) was recorded in May from station1 and the highest (2.70) in the same month from station3, and was the lowest value for the evenness index (J) (0.476) in May was recorded for the station1 and the highest (0.933) in January. The lowest value of richness index (D) (0.868) was obtained in December from station1 and the highest (3.285) in May from station3. The lowest value for Jaccared similarity index (13.25 %) was in October between stations1 and 2 and the highest (100 %) in November and January between stations 1 and 2. Multivariate analysis (CCA) was applied to clarify the relationship between abiotic environmental characteristics and fish distribution in the aquatic environment.

تاثير توزيع النباتات بالحقل في نمو وحاصل الباقلاء Vicia faba L

اسم المؤلف: سارة علي طالب
اسم المشرف: هيثم عبد السلام علي
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - المحاصيل الحقلية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Field experiment was conducted during the winter season 2014 - 2015 in one of the agricultural fields in Sarraji area south of Basra province, just 2 km from the center of the province (soil Texture loamy clay), to determine the best combination of distribution of plants among between Rows and hills, to get the best seed yield, Carried out the experiment design (R.C.B.D) with four replication, two factors the first factor four distances between the Rows (30, 40, 50 and 60 cm) and the second factor three spaces between the hills(10, 15 and 20 cm), the factorial experiment is used with 48 experimental units. Results show moral superiority in most of the traits excelled as distance 30 cm between the Rows at all the distances used to achieve the highest seed yield amounted to 3463.94 kg ha - 1 as this distance hasworked to increase the plant significantly increased since given rise the highest rate of highest plants 122.50 cm and also excelled in the 100 - seed weight at a rate 140.20g, while surpassed the distance of 60 cm in leaf area was at a rate of 0.29 m2 and the highest rate of number pods at plant reached 15.48 pod/ plant and the number of seeds pod - 1 average of 4.37 seeds pod - 1 and high Protein content average of 28.51%. As shown by the results moral superiority of 10 cm seed yield3817.85 kg ha - 1 in 93.73 plant height cm and outperformed the distanceof 20 cm in the number of pods plant - 1 13.43 pod plant and the number of seeds/ pod 3.80 seed pod - 1 in 0.22 m2 leaf area and in protein content in seeds 29.36%, while surpassed the distance of 15 cm in 100seed weight was 98.02g The results showed that there is an interaction between the distance of Rows and the hills are significant in most of the traits and the effect of the interaction is similar to the effect of the main factors as excelled combination 30*10 cm in the sum total of seeds yield to 4067.22 kg ha - 1 as well as excelled in plant height was 131.45 cm and also surpassed 100 seed weight with a means 156.64 g, while the combination 60*20 cm in a number pods of 18.8 pod plant - 1 and the number of seeds/ pod of 5.18 seed pod - 1 and leaf area of the plant 0.35 m2 and in Protein content average of 33.11%.

استخلاص وتوصيف صبغة Astaxanthin من مخلفات الروبيان واختبار فعاليتها المضادة للاكسدة واستعمالها في بعض الانظمة الغذائية == Extraction and Characterization of Astaxanthin Pigment from Shrimp Waste and Tested it as Antioxidant Activity and Using in Some Food System

اسم المؤلف: زينة طارق نعمة الكنعان
اسم المشرف: منير عبود جاسم الطائي | روضة محمود علي العلي
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: الاغذية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The current study aimed to exploit the peels of shrimp in extracting dye, the following results were obtained : shrimp peels contained 1.29% moisture, 1.83% protein, 18.56% fat and 77.82 ash. The astaxanthin dye was extracted and its amount and activity were determined. Five solvents were used to choose the best one in extraction the dye from fresh, dried, boiled and freezed peel. Among all solvents hexane gave less content and activity. Fresh peels gave high content and activity 60.71μg/g and 45.92 % respectively. Mixtures of isopropanol : ether and hexane : acetone also gave high extraction. 90 % acetone showed high extraction of astaxanthin, 79.61μg/g and 98.26% respectively for the activity and content, while results were converged for acetone 90%. Antioxidant activity was measured after 24 hours of incubation, it was increased proportionally with dye concentration and ranged between 83.47 - 98.7% and 2 - 20 mg/ml, respectively. Astaxanthin activity was the best in comparison with standard astaxanthin, BHT, and α - tocopherol, which were 93.76%, 96.75% and 94.50%, respectively at higher concentration. Astaxanthin showed significant antioxidant activity in comparison with standard astaxanthin, BHT, and α - tocopherol, during three months of storage. During the storage period, from the first day to thirteenth antioxidant activity was fixed and ranged between 80.83 - 96.57% at 20 mg/g, while BHT and α - tocopherol activity was between 73.44% - 95.31% and 49.44% - 93.42% respectively at same conditions. Astaxanthin showed high reduction strenth 275%, which was closed to ascorbic acid 278%, while each of BHT, α - tocopherol and citric acid gave reduction force less than the extracted dye, 200 and 190 and 225%, respectively. The pigment gave good ability to chelate Fe (II) at concentration of 89% which was higher than BHT and α - tocopherol, 44.6% and 45.1% respectively, while EDTA, ascorbic acid and citric acid were excellent in chelating Fe (II), 97.7%, 96.9% and 94%, respectively. The capacity of astaxanthin to scavenge hydroxyl radicle was 84.79% which was close to the ascorbic acid and citric acid, 85.45% and 87.39%, respectively, while it was higher than that of BHT and α - tocopherol which have values of 57.45% and 58.13 respectively. The dye showed a clear ability to scavenge the hydrogen peroxide, 83.03% - 89.67%, from a concentration of 12 - 20 mg/ml, which was higher than BHT, α - tocopherol and ascorbic acid at concentration 74.05%, 86.41% and 86.92%, respectively, while it was less than the ability of citric acid to scavenge the radical of hydrogen peroxide that was 92.29%. Astaxanthin was the best in ability to scavenge the radical of the active oxygen in comparison with standard BHT, α - tocopherolSummary الخلاصة - bandascorbic acid, which recorded values of 87%, 71% and 85%, respectively, while it was slightly less than that of citric acid which was 88%. The dye was characterized by thin layer chromatography (TLC), which compared with β - carotene and standard astaxanthin dye and showed three spots closed in their rate of flowing with the standard astaxanthin, and the fourth spot of β - carotene. The values of flowing were 0.35 and 0.55 and 0.73 and 0.97 respectively. The dye was characterized by the infrared spectrum (FTIR), which showed the most important peeks and bands of active functional groups for each of extracted and standard astaxanthin, and it was concluded that they were the same for two dyes. The thermal gravimetric analysis curve (TGA) showed that the extracted dye passed through two stages, the first stage began from evaporate of the water and weight loss at a temperature of 128.17Co, while at the second stage the dye disintegrated at a temperature of 399.29 Co and 85% loss in comparision with standard astaxanthin that needed one stage, 454.37Co and the loss of 84% to reach the disintegration and stabilization phase. The extracted dye was diagnosed by using UV and visible spectrum and compared with the standard astaxanthin, and both of them gave a higher absorbence at wavelength of 565 nm. The determinnation of the cellular toxicity of dye on human solution blood detected that there are no changes in the shape and appearance of blood, this confirmed safety of astaxanthin against decomposition and precipitation of blood. Astaxanthin was used to preserve crude sesame oil that put in two types of containers, dark and transparent, for three months. The peroxide value, value of alansidin, thiobarbituric acid, the value of total oxidation (Totox), conjugated dienes (CD), conjugated trines (CT), and antioxidant activity were estimated. All measures showed significant results for the oil in dark containers in comparesion with oil in transparent. Beef burger was prepared and submitted to sensory evaluation after adding antioxidant to it, stored for 15 days at 4 - 8°C, during this period, peroxide value and TBA value were measured. Cake was prepared and the sensory evaluation was done, then stored for 35 days, also, peroxide value, TBA and acid value were estimated. The concentration of 300 ppm recorded significant results for all treatments and samples that treated with BHT and the blank. So, it was concluded that astaxanthin dye could be used as a natural antioxidants in food preservation to avoid lipid oxidation, and to improve the quality and safety as food supplement with a healthy and beneficial effects.

تاثير فترة الخزن بالتجميد في بعض الصفات النوعية لقطعتي الصدر والفخذ لطائر السمان الياباني == Impact of Freezing Storage Period on some Characteristics Quality of Breact and Thigh Meat of Japanese Quail

اسم المؤلف: زينب علي كاظم شاهر السعيدي
اسم المشرف: رياض كاظم موسى | ماجد حسن عبد الرضا
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The present study was conducted at the Department of Animal Production / College of Agriculture / University of Basrah. The aim was to determine the effect of freezing storage period at ( - 18)ºc for the periods of (0,30,60,90) days on physical , chemical and sensory changes of breact and thigh meat of Japanese quails of both sexes at the age of (42) days . Chemical tests ware moisture % , protein % and ash % as well as the concentrations of free fatty acid and cholesterol concentration . Physical tests included pH, total dissolved nitrogen % , protein and non - protein tryptophan , thyrosin / tryptophan coefficient and loss percent at cooking . Sensory tests were color , flaver , tenderness , juiciness and total acceptance . The results can be summarized as follow : 1 - A significant (p≤ 0.05) decrease in moisture % and protein %, and increase (p≤ 0.05) in fat % and ash % of breact and thigh of both sexes as storage period advanced .2 - There was a higher loss in moisture % of breact cut of male carcasses as storage period advanced in comparison with breact cut of female carcass . However percentage of losing in female thigh was higher than that of male thigh .3 - Decreasing percent of protein in preceding storage period of breact and thigh cuts of male carcasses were higher than that of female abreact and thigh cuts .4 - An increase in fat % of female breact with preceding storage period was higher than that of males in contrast with thigh of male and female .5 - Breact and thigh cut of male carcasses recorded higher ash% in storage advances .6 - The was a significant (p≤ 0.05) decrease in essential and non - essential amino acids concentration of breact and thigh as in flounced by storage period in comparison with fresh carcasses .7 - There was a significant (p≤ 0.05) increase in percentage of saturated fatty acid ( palmatic and stearic ) and a significant (p≤ 0.05) decrease in non - saturated fatty acid ( palmatic , oleic and lionelic ) of breact and thigh cuts of males and females in proceeding of storage period .8 - Essential amino acids ( methonin , isolucein , phynel alinane and valin ) and non - essential ( alnine , argnin , ceren , prolin , thyrosin and glysin ) losing rate of breact and thigh cuts of male carcasses were higher in storage advance in comparison with those of females.9 - Peroxide and free fatty acid and cholesterol concentration of abreact and thigh cuts of both sexes showed significant (p≤ 0.05) increase with preceding period of storage .10 - There were significant (p≤ 0.05) decrease in pH and increase in total dissolved nitrogen , protein and non - protein tryptophan ,tyrosine / tryptophan coefficient and loss% during cooking of abreact and thigh cuts of carcasses of both sexes in advanced storage period .11 - There was a significant (p≤ 0.05) decrease in sensory characteristics ( color , flavor , juice mess , tenderness and total acceptance of abreact and thigh cuts of both sexes in advance storage period .

تقييم كفاءة مستحضر احيائي للفطر Trichoderma viride والاسمدة العضوية في مكافحة مرضي الذبول الفيوزارمي وتبقع الاوراق في الطماطا

اسم المؤلف: زهراء عبد اللطيف جاسم العقبي
اسم المشرف: ضياء سالم علي الوائلي
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: النبات - الامراض
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: اجريت الدراسة في قسم وقاية النبات - كلية الزراعة - جامعة البصرة وقد هدفت الدراسة الى تقييم كفاءة المستحضر الاحيائي للفطر Trichoderma viride والاسمدة العضوية في مكافحة مرض الذبول الفيوزارمي المتسبب عن الفطر Fusarium oxysporum f.sp. lycopersici ومرض تبقع الاوراق المتسبب عن الفطر Alternaria alternata اللذين يصيبان نبات الطماطا ، والكشف عن بعض المركبات الفعالة التي ينتجها المعاملات المختلفة باستخدام جهاز GC - mass . اوضحت نتائج اختبار كفاءة المستحضر الاحيائي للفطر T.viride ضد الفطرين الممرضين F.o.f.sp. lycopersici وA.alternata في المختبر ان نسبة التثبيط 53.01 قد بلغت 62.65 و65.47 % على التوالي . بينت نتائج تطبيق المستحضر الاحيائيT.viride والاسمدة العضوية في مكافحة الفطر F.o. f.sp. lycopersici في الاصص ان اقل شدة اصابة كانت في المعاملة المستحضر الاحيائي وسماد الابقار المخمر M1Tv اذ بلغت 0% مقارنة بمعاملة السيطرة البالغة 80.33 % , وكان اعلى ارتفاع للنباتات في المعاملة M1Tv اذ بلغ 77 سم مقارنة بمعاملة السيطرة البالغة 47.33 سم وكذلك سجلت المعاملة ذاتها اعلى وزن طري للمجموع الخضري اذ بلغت 67 غم مقارنة بمعاملة السيطرة البالغة 28.50غم ، وسجلت اعلى انتاجية للنبات اذ بلغ معدل الانتاج فيها 160.25غم مقارنة بمعاملة السيطرة البالغة 34.14غم . اظهرت نتائج التجربة الحقلية ان اقل شدة اصابة بالفطرين F.o.fsp lycopersici وA.alternata كانت في المعاملة M1Tv اذ بلغت 6.25 و9.33 % مقارنة بمعاملة السيطرة البالغة 85.71 و51.67 % على التوالي . وكان اعلى ارتفاع للنبات في المعاملة M1Tv واذ بلغ 89.84 سم مقارنة بمعاملة السيطرة البالغة 58.67 سم . سجلت المعاملة M1Tv اعلى وزن طري للمجموع الخضري 212.17 غم مقارنة بمعاملة السيطرة 53.67 غم وكذلك سجلت اعلى معدل انتاجية اذ بلغ 592.63 غم مقارنة بمعاملة السيطرة 211.37 غم . تم استخدام تقنية GC - mass للكشف عن المركبات في المعاملات المستخدمة في التجربة وقد وجد ان المعاملة M1Tv انفردت باحتواءها على المادة 4 - Cloro - 3 - hexyltrahydro - 2H - pyran في حين وجدت المادتين 1,5,9 - Cyclododecanetriol وpropanol 1 2 - Cyclohexyl - في كل من المعاملة M1Tv ومعاملة المستحضر الاحيائي فقط Tv . ويعتقد ان لهذه المواد دور فعال في تقليل نسبة الاصابة وتحسين مؤشرات النمو والانتاجية لنبات الطماطا

استخدام جين الميتوكوندريا COI في تمييز انواع لحوم مختلفة من الحيوانات اعتمادا على تقانة PCR - RFLP == The use of mitochondrial gene COI in Identifying different animal types of meat based on PCR - RFLP technique

اسم المؤلف: زهراء رياض محمود الموسوي
اسم المشرف: اسعد يحيى عايد
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في مختبر د. طالب احمد جايد للوراثة الجزيئية في قسم الانتاج الحيواني التابع لكلية الزراعة في جامعة البصرة. اذ جمعت عينات اللحوم الطازجة والمعلبة من الابقار والجاموس والاغنام والماعز والدجاج والديك الرومي، بشكل عشوائي، من مناطق مختلفة في محافظة البصرة بالاضافة الى عينات من دم الجمال. واجريت هذه الدارسة للتعرف على لحوم الانواع الحيوانية المختلفة (عدا الجمال) ومن هنا تاتي اهمية الاعتماد على كشف الانواع من خلال استهداف الحامض النووي الرايبوزي منقوص الاوكسجين DNA عبر تطبيق تقانة PCR - RFLP. تم استخلاص الحامض النووي منقوص الاوكسجين DNA من نسيج اللحوم 0.2) غم) والدم باستعمال عدة استخلاص الدنا (Invitrogen). اما فيما يتعلق بنقاوة عينات DNA المستعملة في الدراسة فقد قيست اعتمادا على الطول الموجي 260 - 280، حيث تركيز DNA 1.8 - 2.0 نانوغرام.استخدم تفاعل البلمرة التسلسلي PCRلتضخيم جزء من المورثة الميتوكونديرية mt COI DNA باستعمال بادئ عام، ولتحديد هوية الانواع استخدمت تقانة التمييز على اساس تعدد طرز اطوال قطع التقييد PCR - RFLP من خلال استخدام اربعة انزيمات قطع محددة( Hpa II وAva II وRsa Iو Taq I ) والتي تقطع وفقا لتتالي تسلسل DNA الخاص بكل نوع.  ويمكن تلخيص نتائج هذه الدراسة بما ياتي : 1 - اعطى منتج التضخيم لجين COI الحزمة 710 زوجا قاعديا ولجميع الانواع كونه بادئ عام.2 - ميز انزيم TaqIالماعز حيث انتج الحزمة 650زوجا قاعديا كذلك الجمال اذ انتج حزمتين بحجم 303و 403ازواج قاعدي بينما لم يميز بين لحوم الجاموس والابقار والدجاج والديك الرومي بسبب حصولهما على الحزمة 637 زوجا قاعديا عند الهضم في حين لم يتم الحصول على حزم من لحوم الاغنام. 3 - تمكن انزيم HpaII من توليد ملف تعريفي خاص لتمييز انواع اللحوم جميعا على حدة اذ انتج الحزمة 480في الابقار و520 في الجاموس و372 - 336في الاغنام و300 - 410 في الماعز و333 - 234 في الدجاج و533 - 177 في الديك الرومي و130 246 - 213 - زوجا قاعديا في الجمال.4 - انتج انزيم Rsa I انماطا مميزة لجميع الانواع عدا الماعز والدجاج، اذ انتج منتج هضم كل من الاغنام 300 - 350والجمال130 - 580والابقار153 - 193 - 337 والجاموس600 والديك الرومي 214 - 481زوجا قاعديا.5 - انتج انزيم Ava II حزمتين للجمال 224 - 485وحزمة واحدة للاغنام بحجم650 وحزمة بحجم 600 زوجا قاعديا للجاموس. | This study was conducted in the Laboratory of Dr.Talib Ahmed Jaayid Molecular Genetics, Department of Animal Production, College of Agriculture, University of Basra. Cow, buffalo, sheep, goat ,chicken, turkey meat (fresh or canned) & camels blood samples are randomly collected from different areas of Basra province. The aim of this study is to identify the animal species from their meat except camels by targeting the deoxyribonucleic acid (DNA) through PCR - RFLP technique. DNA is extracted from the tissue of meat (0.2)g and blood using kit (invitrogen). With regard to the purity of DNA sample used in this study it has been measured depending on wave length of 260 - 280 and 1.8 - 2.0 ng DNA concentration.Polymerase chain reaction was used to amplify the part of the mitochondrial gene (mt COI DNA) by universal primers and to identify the species origin PCR - RFLP technique applied through the use of four restriction enzymes (Ava II, Rsa I, Taq I, Hpa II) that cut according to the following bases of each type of DNA. The results of the current study can be summarize as follows : 1 - PCR product gives the fragment 710 bp in all species for being a universal primers.2 - TaqI enzyme distinguish goats with a fragment of (650) bp, (303 - 403) bp in camels. While it doesn’t distinguish between buffaloes , cow, chicken , turkey meat. As they obtain the same fragment (637) bp when digestion. Whereas, no fragments are obtained from Sheep meat.3 - The enzyme HpaII enables to generate a special file definitions to distinguish all types of meat separately if they yield the fragment of (480) bp in cow, (520) bp in buffaloes, (300 - 410) bp in sheep, (336 - 372) bp in goats, (234 - 333)bp in chicken, (533 - 177) bp in Turkey and (130 - 213 - 246) bp in camels. 4 - Rsa I enzyme generates patterns characteristic for all species except goats and chickens. Whereas, fragments are obtained in sheep (300 - 350) bp, cow (135 - 193 - 337) bp, buffaloes (600) bp, turkey (214 - 481) bp & (130 - 580) bp in camels.5 - AvaII enzyme generates two fragments in buffaloes (600) bp, (224 - 485) bp in camels and (650) bp in Sheep meat

تاثير الرش بالحديد والتسميد الارضي بالبوتاسيوم في نمو وحاصل حنطة الخبز Triticum aestivum L == Effect of Iron foliar applications and Potassium ground Fertilizer on Growth and Yields of bread Wheat Triticum aestivum L.)

اسم المؤلف: رونق منصور جازع العلوي
اسم المشرف: كريم حنون محسن | بهاء الدين محمد محسن
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: النبات - المحاصيل الحقلية
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: A field experiment was conducted during The winter season of 2015 at farmer in Al - Dear district, Alshenana (65 km north of Basra) in a loamy clay soil in order to study the effect of foliar nutrition by iron and potassium fertilization on growth and yield quality of wheat (Triticum aestivum L.) The treatment were the spray of iron in three concentrations (0,40,80 mg Fe L - 1) which were (Fe0, Fe1, Fe2) and four levels of potassium fertilizer (0, 20, 40,60 kg K ha - 1) which were (K0, K1, K2, K3). The statistical system was split plot design in randomized complete block design (R.C.B.D) in three replications the main plot was Fe and the sub plot was potassium. The cultivar that was used Bohooth 22 and the total experimental units were 36.The result showed the Increase Fe concentrations caused increased period from culture to 75% flowering, plant height, number of tillers, flag leaf area and length of spike. Also, crop content increased and the increase of bio yield and biological yield where the high concentration of Fe produced grain yield 5.86 tons/ha.Potassium fertilizer increased plant height, number of the tillers, flag leaf area and spike length. The high levels of potassium increased yield content which intern increased bio and biological yield. The high level of potassium increased grain yields to 7.01 tons /ha. The interaction between the high Fe and potassium level gave significant effect on most characteristic, the (Fe2k3) caused high grain yield, biological yield content which were 7.17 tons /ha, 13.70 tons /ha.

تاثير اضافة الموننسين في معامل الهضم ومعايير الكرش والصفات الانتاجية للحملان العرابية == Effect of Adding Monensin on Digestion coefficient , Rumen Parameters and Productive Traits and of Arabi Lambs

اسم المؤلف: رشيد حميد عبد الله العايدي
اسم المشرف: عماد فلاح حسن الجاسم | هناء علي جبار الغالبي
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This study was undertaken in the Field of Animal of the Faculty of Agriculture in Basra University for the period from 1/11/2015 to 10/2/2016. The study included the growth and digestion experiments. The digestion trail included 12 Arabi lambs ranging in age between 5 - 6 months with a mean weight 21 kg were randomly divided into three groups of four animals for each treatment. Ration submitted on the basis of the 3% of body weight, their quantities to be modified on the basis of the new weight of each group every two weeks. .Ration was provided twice daily, in the morning at 8 a.m. and in the evening at 4 pm. Amount of feed and the remaining was calculated daily for the purpose of calculating the amount of feed consumption. At the beginning of the experiment lambs were introduced for adaptation period of 10 days and then weighed, the registration of primary weight as initial weight. The first studied ration was considered as control (without any addition). The second ration was control added to monensin by 100 mg/kg. The third ration was control added to monensin by 200 mg/kg. The results obtained can be summarized as follows : 1 - Final weight was significantly affected by adding monensin with the superiority of the second treatment when adding monensin at a level of 100 mg/kg dry material which were recorded a 33.36 kg, while those of the first and the third groups, which registered 28.61 and 29.20 kg respectively. As for the overall growth rate second treatment exceeded (11.33 kg) significantly (P<0.05) first and third groups (4.75 and 6.01 Kg respectively).2 - The largest amount of feed consumption on the basis of dry matter was shown by second group (65.24 kg), followed by the control,Bwhich reached 60.37 kg. Feed consumption declined (P<0.05) with anincrease in the proportion of monensin until it reached a value of 56.25 kg showed by third treatment, which differed significantly (P<0.05) from the first treatment (control) and second treatment.3 - The best feed conversion efficiency of the diet recorded by the lambs of second treatment (6.97 kg dry matter consumed/ kg weight gain) which was mathematically less than that of control group (12.71 kg dry matter consumed/ kg weight gain), while it differed significantly from that of the third treatment (9.36 kg dry matter consumed/ kg weight gain).4 - The superiority (P<0.05) of the digestion rate of dry matter of the third treatment lambs (66.25 %) in comparison with those of second and first groups (58.52 and 57.02% respectively). While there was a significant fall (P<0.05) in protein digestibility (32.59%) of the third treatment in comparison with 51.27 and 56.15% of the first and second group respectively. There were no significant differences in the average digestibility of fat fibers of the three treatments.5 - A significant fall (P<0.05) in the value of the pH of rumen fluid of lambs received 200 mg monensin/kg dry matter which stood at 6.15 compared with the control group and the second group which reached 6.46 and 6.50 respectively.6 - The lack of significant differences in the number of total bacteria and cellulolytic bacteria of lambs in all treatments regardless of the level of the monensin.7 - Significant decrease in methane and ammonia concentrations in rumen fluid due to adding 100 and 200 mg of monensin (15.19, 14.86 and 12.19 l methane/day and 103.30, 92.75 and 89.45 mg ammonia/l for control, 100 and 200 mg monensin respectively).8 - The lack of significant differences in the mean value of blood glucose, total protein, cholesterol and triglycerides of different treatments.9 - The existence of significant differences (P<0.05) between the second treatment and the others in the average hot carcass weight (17.26 kg) in comparison with 13.12 and 15.29 kg of the first and third treatment respectively.10 - The superiority of thigh and loin cuts weight (P<0.05) of the 2nd treatment (5800, 2136.6 gm) over the mean of control and 3rd treatment (4550.0 and 4630 gm of thigh and 1476.6 and 1735.0 gm of loin respectively).11 - There were no significant differences in the mean of internal organs weight and heart, kidney, pelvic and abdominal fat weights among all treatments, 12 - Significant differences were exist in the mean of carcass length and chest depth of lambs of 3rd treatment (73.0 cm and 26.33 cm) in comparison with those of second and control group (66.33, 61.33 cm carcass length and 25.75, 21.00 cm chest depth).
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