عزل وتشخيص بكتيريا . Vibrio spp من بعض الاسماك المتداولة في الاسواق المحلية لمدينتي البصرة والناصرية ودراسة بعض خواصها == Isolation and Identification of Vibrio spp. from some fish proffered in local markets of Basrah and Nasiriyah city and study some properties
Author name:
عذارء عودة حسين الجبوري
Supervisor name:
خديجة صادق جعفر الحسيني | منال بادي صالح التميمي
General topic:
Agricultural sciences
Specific topic:
Food
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1826 - p.pdf
Abstract:
The study included isolation and identification Vibrio spp. bacteria that taken from fresh and frozen fish (Tenualosa ilisha , Hypophthalmichthys molitrix, Barbus sharpey and Spondyliosoma cantharus). 210 fishes were collected from Basrah market (Big market in Basrah, Al - Ashar, Al - Tanoma and 5 mile) while 150 fishes were collected of Hypophthalmichthys molitrix, Barbus sharpeyand Spondyliosoma cantharus) from Nasiriyah market (Big market and Harj market) this collecting process of samples took a period from 02.June till 30.November - 2013.Fishes were brought to laboratory and samples were taken from it and planted directly on biological media (Nutrient agar, Blood agar, MacConkey agar and selective medium (Thiosulphate Citrate Bile salt Sucrose Agar TCBS) then the petri dishes were incubated aerobically at temperature 37cº for 18 - 24 hours. 30 positive samples were identified of Vibrios depending on colony shape, cell under microscope, catalase test, oxidase test and motility test, afer that the types were identified depending on the biochemical tests and by using API20E kit, which led to identifying two kinds of Vibrios bacteria : Vibrio cholera and Vibrio fluvialis. These two bacteria were isolated from fresh fishes more than frozen fishes.Study observed that the Vibrios isolation percentage of these two bacteria in Basrah market was 26% (15 isolates) while the isolation percentage was 21% in Nasiriyah market (63 isolates).Sensitivity and antibiotics tests were done, results showed that all Vibrios cholera bacteria had 100% resistant against Aztreonem, while some of it had low resistant which was about 45% against Amoxicillin - clavulanic Acid and Nalidixic Acid, all Vibrio fluvialis bacteria isolates had resistant against Aztreonem, Amoxicillin - clavulanic Acid and Nalidixic Acid which was 100%, 75% and 25% respectively, while all VibrioAcholera and Vibrio fluvialis isolates were sensitive 100% against Amikacin, Ampicillin, Chloram - phenicol, Ciprofloxacin, Tetarcycline, Trimethoprim /Sulpha - methoxazole.Vibrio cholera and Vibrio fluvialis bacteria isolates were 100% tolerant for acidity at pH 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0, while Vibrio cholera bacteria was 60% tolerant to the acidity at pH 4.5, and Vibrio fluvialis bacteria was more resistant to the acidity of 20% and 50% tolerant at pH 4.0 and 4.5 respectively.The study showed to the ability of Vibrio cholera bacteria to resist the salinity reached 60% and 100% at concentrations 6% and 7% respectively, but it didn't grow on concentrations 8% and 9%.Vibrio fluvialis bacteria was resistant for the salinity in all the concentrations with different percentages reached to 100% at concentrations 6%, 7% and 40% at concentration 8%, while at concentration 9% there was only one sample had resistant of 20% and it was from frozn sample of Beni fish which taken from Big market in Basrah.All isolates from different sources could grow in 25 and 37 cº and in room temperature 15 cº during doing this experiment, while some isolates of Vibrios bacteria could grow with low density in 5 cº, while it couldn't grow in - 20 cº.By using Real Time (PCR) technology for detecting hemolysim enzyme gene in Vibrio spp., that 27 samples from Vibrio cholera bacteria and 3 samples from Vibrio fluvialis bacteria had this enzyme which analyzes red blood cell (RBC) and considered one of toxins that belongs to Vibrio spp. which has a role in occurrence of disease.
